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Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)

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2012
3020.pdf (977.9Kb)
Authors
Savić, Jelena
Dragićević, Ivana
Pantelić, D.
Oljača, Jasmina
Momčilović, Ivana
Article (Published version)
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Abstract
We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to he...at-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.

Keywords:
heat stress / heat tolerance / potato / small heat shock proteins / sHSP electrolyte leakage assay
Source:
Archives of Biological Sciences, 2012, 64, 1, 135-144
Publisher:
  • Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
  • Development and application of protein markers for heat tolerance screening in potato (RS-31049)

DOI: 10.2298/ABS1201135S

ISSN: 0354-4664

WoS: 000303697400016

Scopus: 2-s2.0-84863745819
[ Google Scholar ]
11
11
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/3023
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Poljoprivredni fakultet
TY  - JOUR
AU  - Savić, Jelena
AU  - Dragićević, Ivana
AU  - Pantelić, D.
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3023
AB  - We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)
EP  - 144
IS  - 1
SP  - 135
VL  - 64
DO  - 10.2298/ABS1201135S
UR  - conv_4901
ER  - 
@article{
author = "Savić, Jelena and Dragićević, Ivana and Pantelić, D. and Oljača, Jasmina and Momčilović, Ivana",
year = "2012",
abstract = "We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)",
pages = "144-135",
number = "1",
volume = "64",
doi = "10.2298/ABS1201135S",
url = "conv_4901"
}
Savić, J., Dragićević, I., Pantelić, D., Oljača, J.,& Momčilović, I.. (2012). Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(1), 135-144.
https://doi.org/10.2298/ABS1201135S
conv_4901
Savić J, Dragićević I, Pantelić D, Oljača J, Momčilović I. Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences. 2012;64(1):135-144.
doi:10.2298/ABS1201135S
conv_4901 .
Savić, Jelena, Dragićević, Ivana, Pantelić, D., Oljača, Jasmina, Momčilović, Ivana, "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)" in Archives of Biological Sciences, 64, no. 1 (2012):135-144,
https://doi.org/10.2298/ABS1201135S .,
conv_4901 .

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