TY  - JOUR
AU  - Jakovljević, Violeta D.
AU  - Vrvić, Miroslav M.
AU  - Vrbničanin, Sava
AU  - Sarić-Krsmanović, Marija
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5766
AB  - This work was conceptualized with the goal to investigate the phytochemical, free radical scavenging and antifungal profile of Cuscuta campestrisYunck. seeds. Total phenolics, amino acid and carbohydrate contents were evaluated in ethanolic, acetone and chloroform extract. Effective antioxidant activity was evaluated throughout seven antioxidant methods. The antifungal activity was assessed against eight fungal strains and Candida albicans. The results showed total phenol, flavonoid, flavonols and phenolic acids contents in amount of 1.51-6.35mg GAE/mL, 78-425g RU/mL, 1.04-2.98mg QU/g and 12.01-30.58g CAE/mL, respectively. The total amino acids and carbohydrates content ranged from 8.29 to 185.45g Gly/mL and from 0.05 to 0.12g Glu/mL. The ethanolic extract showed the best antioxidant activity in phosphomolybdenum, DPPH free radical scavenging, ferric reducing power and lipid peroxidation assays. The best activity in ferrous ion chelating and H2O2 assays had the acetone extract, whereas the best hydroxyl radical scavenging activity was observed with the chloroform extract. The ethanolic extract at a concentration of 6mg/mL proved to be the most effective antimycotic, since it inhibited the growth of all tested fungi except Penicillium verrucosum. The obtained results indicate that C.campestris seeds could be attributed to a potential source of natural antioxidants in food and pharmaceutical products.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds
IS  - 8
VL  - 15
DO  - 10.1002/cbdv.201800174
ER  - 

@article{
author = "Jakovljević, Violeta D. and Vrvić, Miroslav M. and Vrbničanin, Sava and Sarić-Krsmanović, Marija",
year = "2018",
abstract = "This work was conceptualized with the goal to investigate the phytochemical, free radical scavenging and antifungal profile of Cuscuta campestrisYunck. seeds. Total phenolics, amino acid and carbohydrate contents were evaluated in ethanolic, acetone and chloroform extract. Effective antioxidant activity was evaluated throughout seven antioxidant methods. The antifungal activity was assessed against eight fungal strains and Candida albicans. The results showed total phenol, flavonoid, flavonols and phenolic acids contents in amount of 1.51-6.35mg GAE/mL, 78-425g RU/mL, 1.04-2.98mg QU/g and 12.01-30.58g CAE/mL, respectively. The total amino acids and carbohydrates content ranged from 8.29 to 185.45g Gly/mL and from 0.05 to 0.12g Glu/mL. The ethanolic extract showed the best antioxidant activity in phosphomolybdenum, DPPH free radical scavenging, ferric reducing power and lipid peroxidation assays. The best activity in ferrous ion chelating and H2O2 assays had the acetone extract, whereas the best hydroxyl radical scavenging activity was observed with the chloroform extract. The ethanolic extract at a concentration of 6mg/mL proved to be the most effective antimycotic, since it inhibited the growth of all tested fungi except Penicillium verrucosum. The obtained results indicate that C.campestris seeds could be attributed to a potential source of natural antioxidants in food and pharmaceutical products.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds",
number = "8",
volume = "15",
doi = "10.1002/cbdv.201800174"
}

Jakovljević, V. D., Vrvić, M. M., Vrbničanin, S.,& Sarić-Krsmanović, M.. (2018). Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 15(8).
https://doi.org/10.1002/cbdv.201800174

Jakovljević VD, Vrvić MM, Vrbničanin S, Sarić-Krsmanović M. Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds. in Chemistry & Biodiversity. 2018;15(8).
doi:10.1002/cbdv.201800174 .

Jakovljević, Violeta D., Vrvić, Miroslav M., Vrbničanin, Sava, Sarić-Krsmanović, Marija, "Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds" in Chemistry & Biodiversity, 15, no. 8 (2018),
https://doi.org/10.1002/cbdv.201800174 . .

TY  - JOUR
AU  - Jakovljević, Violeta D.
AU  - Vrvić, Miroslav M.
AU  - Vrbničanin, Sava
AU  - Sarić-Krsmanović, Marija
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4699
AB  - This work was conceptualized with the goal to investigate the phytochemical, free radical scavenging and antifungal profile of Cuscuta campestrisYunck. seeds. Total phenolics, amino acid and carbohydrate contents were evaluated in ethanolic, acetone and chloroform extract. Effective antioxidant activity was evaluated throughout seven antioxidant methods. The antifungal activity was assessed against eight fungal strains and Candida albicans. The results showed total phenol, flavonoid, flavonols and phenolic acids contents in amount of 1.51-6.35mg GAE/mL, 78-425g RU/mL, 1.04-2.98mg QU/g and 12.01-30.58g CAE/mL, respectively. The total amino acids and carbohydrates content ranged from 8.29 to 185.45g Gly/mL and from 0.05 to 0.12g Glu/mL. The ethanolic extract showed the best antioxidant activity in phosphomolybdenum, DPPH free radical scavenging, ferric reducing power and lipid peroxidation assays. The best activity in ferrous ion chelating and H2O2 assays had the acetone extract, whereas the best hydroxyl radical scavenging activity was observed with the chloroform extract. The ethanolic extract at a concentration of 6mg/mL proved to be the most effective antimycotic, since it inhibited the growth of all tested fungi except Penicillium verrucosum. The obtained results indicate that C.campestris seeds could be attributed to a potential source of natural antioxidants in food and pharmaceutical products.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds
IS  - 8
VL  - 15
DO  - 10.1002/cbdv.201800174
ER  - 

@article{
author = "Jakovljević, Violeta D. and Vrvić, Miroslav M. and Vrbničanin, Sava and Sarić-Krsmanović, Marija",
year = "2018",
abstract = "This work was conceptualized with the goal to investigate the phytochemical, free radical scavenging and antifungal profile of Cuscuta campestrisYunck. seeds. Total phenolics, amino acid and carbohydrate contents were evaluated in ethanolic, acetone and chloroform extract. Effective antioxidant activity was evaluated throughout seven antioxidant methods. The antifungal activity was assessed against eight fungal strains and Candida albicans. The results showed total phenol, flavonoid, flavonols and phenolic acids contents in amount of 1.51-6.35mg GAE/mL, 78-425g RU/mL, 1.04-2.98mg QU/g and 12.01-30.58g CAE/mL, respectively. The total amino acids and carbohydrates content ranged from 8.29 to 185.45g Gly/mL and from 0.05 to 0.12g Glu/mL. The ethanolic extract showed the best antioxidant activity in phosphomolybdenum, DPPH free radical scavenging, ferric reducing power and lipid peroxidation assays. The best activity in ferrous ion chelating and H2O2 assays had the acetone extract, whereas the best hydroxyl radical scavenging activity was observed with the chloroform extract. The ethanolic extract at a concentration of 6mg/mL proved to be the most effective antimycotic, since it inhibited the growth of all tested fungi except Penicillium verrucosum. The obtained results indicate that C.campestris seeds could be attributed to a potential source of natural antioxidants in food and pharmaceutical products.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds",
number = "8",
volume = "15",
doi = "10.1002/cbdv.201800174"
}

Jakovljević, V. D., Vrvić, M. M., Vrbničanin, S.,& Sarić-Krsmanović, M.. (2018). Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 15(8).
https://doi.org/10.1002/cbdv.201800174

Jakovljević VD, Vrvić MM, Vrbničanin S, Sarić-Krsmanović M. Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds. in Chemistry & Biodiversity. 2018;15(8).
doi:10.1002/cbdv.201800174 .

Jakovljević, Violeta D., Vrvić, Miroslav M., Vrbničanin, Sava, Sarić-Krsmanović, Marija, "Phytochemical, Free Radical Scavenging and Antifungal Profile of Cuscuta campestris Yunck. Seeds" in Chemistry & Biodiversity, 15, no. 8 (2018),
https://doi.org/10.1002/cbdv.201800174 . .

TY  - JOUR
AU  - Racić, Gordana
AU  - Kormoczi, Peter
AU  - Kredics, Laszlo
AU  - Raičević, Vera
AU  - Mutavdzić, Beba
AU  - Vrvić, Miroslav M.
AU  - Panković, Dejana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4384
AB  - Influence of edaphic factors and metal content on diversity of Trichoderma species at 14 different soil sampling locations, on two depths, was examined. Forty-one Trichoderma isolates from 14 sampling sites were determined as nine species based on their internal transcribed spacer (ITS) sequences. Our results indicate that weakly alkaline soils are rich sources of Trichoderma strains. Also, higher contents of available K and P are connected with higher Trichoderma diversity. Increased metal content in soil was not inhibiting factor for Trichoderma species occurrence. Relationship between these factors was confirmed by locally weighted sequential smoothing (LOESS) nonparametric smoothing analysis. Trichoderma strain (Szeged Microbiology Collection (SZMC) 22669) from soil with concentrations of Cr and Ni above remediation values should be tested for its potential for bioremediation of these metals in polluted soils.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.
EP  - 3386
IS  - 4
SP  - 3375
VL  - 24
DO  - 10.1007/s11356-016-8067-1
ER  - 

@article{
author = "Racić, Gordana and Kormoczi, Peter and Kredics, Laszlo and Raičević, Vera and Mutavdzić, Beba and Vrvić, Miroslav M. and Panković, Dejana",
year = "2017",
abstract = "Influence of edaphic factors and metal content on diversity of Trichoderma species at 14 different soil sampling locations, on two depths, was examined. Forty-one Trichoderma isolates from 14 sampling sites were determined as nine species based on their internal transcribed spacer (ITS) sequences. Our results indicate that weakly alkaline soils are rich sources of Trichoderma strains. Also, higher contents of available K and P are connected with higher Trichoderma diversity. Increased metal content in soil was not inhibiting factor for Trichoderma species occurrence. Relationship between these factors was confirmed by locally weighted sequential smoothing (LOESS) nonparametric smoothing analysis. Trichoderma strain (Szeged Microbiology Collection (SZMC) 22669) from soil with concentrations of Cr and Ni above remediation values should be tested for its potential for bioremediation of these metals in polluted soils.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.",
pages = "3386-3375",
number = "4",
volume = "24",
doi = "10.1007/s11356-016-8067-1"
}

Racić, G., Kormoczi, P., Kredics, L., Raičević, V., Mutavdzić, B., Vrvić, M. M.,& Panković, D.. (2017). Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(4), 3375-3386.
https://doi.org/10.1007/s11356-016-8067-1

Racić G, Kormoczi P, Kredics L, Raičević V, Mutavdzić B, Vrvić MM, Panković D. Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp.. in Environmental Science and Pollution Research. 2017;24(4):3375-3386.
doi:10.1007/s11356-016-8067-1 .

Racić, Gordana, Kormoczi, Peter, Kredics, Laszlo, Raičević, Vera, Mutavdzić, Beba, Vrvić, Miroslav M., Panković, Dejana, "Effect of the edaphic factors and metal content in soil on the diversity of Trichoderma spp." in Environmental Science and Pollution Research, 24, no. 4 (2017):3375-3386,
https://doi.org/10.1007/s11356-016-8067-1 . .

TY  - JOUR
AU  - Kekez, Branka
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Pavlović, Vladimir
AU  - Beskoski, Vladimir
AU  - Popović, Aleksandar
AU  - Vrvić, Miroslav M.
AU  - Nikolić, Vladimir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4034
AB  - Novel macromolecular graft copolymers were synthesized by reaction of the hydroxyl groups of the microbial polysaccharide levan, produced using Bacillus licheniformis, with polystyrene (Lev-g-PS). Synthesis was performed by the free radical reaction using potassium persulfate (PPS) as initiator. The prepared copolymer was characterized by FTIR, SEM, TG/DTA, XRD and C-13 NMR. The influence of the different conditions (reaction temperature, air or nitrogen atmosphere, reaction time, type of amines and ascorbic acid (AA) concentration) on the grafting reaction was investigated. Results showed that maximum percentage of grafting (58.1%) was achieved at a reaction temperature 70 degrees C, in a nitrogen atmosphere and using dimethylethanolamine (DMEA) as the amine activator. On the basis of the obtained results, the likely reaction mechanism was proposed. Synthesized copolymers have better thermal stability in comparison with their initial components. Copolymers such as Lev-g-PS could potentially have many applications, such as compatibilizers and material for membranes.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Synthesis and characterization of a new type of levan-graft-polystyrene copolymer
EP  - 29
SP  - 20
VL  - 154
DO  - 10.1016/j.carbpol.2016.08.001
ER  - 

@article{
author = "Kekez, Branka and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Pavlović, Vladimir and Beskoski, Vladimir and Popović, Aleksandar and Vrvić, Miroslav M. and Nikolić, Vladimir",
year = "2016",
abstract = "Novel macromolecular graft copolymers were synthesized by reaction of the hydroxyl groups of the microbial polysaccharide levan, produced using Bacillus licheniformis, with polystyrene (Lev-g-PS). Synthesis was performed by the free radical reaction using potassium persulfate (PPS) as initiator. The prepared copolymer was characterized by FTIR, SEM, TG/DTA, XRD and C-13 NMR. The influence of the different conditions (reaction temperature, air or nitrogen atmosphere, reaction time, type of amines and ascorbic acid (AA) concentration) on the grafting reaction was investigated. Results showed that maximum percentage of grafting (58.1%) was achieved at a reaction temperature 70 degrees C, in a nitrogen atmosphere and using dimethylethanolamine (DMEA) as the amine activator. On the basis of the obtained results, the likely reaction mechanism was proposed. Synthesized copolymers have better thermal stability in comparison with their initial components. Copolymers such as Lev-g-PS could potentially have many applications, such as compatibilizers and material for membranes.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Synthesis and characterization of a new type of levan-graft-polystyrene copolymer",
pages = "29-20",
volume = "154",
doi = "10.1016/j.carbpol.2016.08.001"
}

Kekez, B., Gojgić-Cvijović, G., Jakovljević, D., Pavlović, V., Beskoski, V., Popović, A., Vrvić, M. M.,& Nikolić, V.. (2016). Synthesis and characterization of a new type of levan-graft-polystyrene copolymer. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 154, 20-29.
https://doi.org/10.1016/j.carbpol.2016.08.001

Kekez B, Gojgić-Cvijović G, Jakovljević D, Pavlović V, Beskoski V, Popović A, Vrvić MM, Nikolić V. Synthesis and characterization of a new type of levan-graft-polystyrene copolymer. in Carbohydrate Polymers. 2016;154:20-29.
doi:10.1016/j.carbpol.2016.08.001 .

Kekez, Branka, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Pavlović, Vladimir, Beskoski, Vladimir, Popović, Aleksandar, Vrvić, Miroslav M., Nikolić, Vladimir, "Synthesis and characterization of a new type of levan-graft-polystyrene copolymer" in Carbohydrate Polymers, 154 (2016):20-29,
https://doi.org/10.1016/j.carbpol.2016.08.001 . .

TY  - CONF
AU  - Pešić, Mirjana
AU  - Barać, Miroljub
AU  - Stanojević, Sladjana
AU  - Vrvić, Miroslav M.
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4115
PB  - Springer, New York
C3  - Emerging and Traditional Technologies for Safe, Healthy and Quality Food
T1  - Heat-Induced Casein-Whey Protein Interactions in Caprine Milk: Whether Are Similar to Bovine Milk?
EP  - 175
SP  - 163
DO  - 10.1007/978-3-319-24040-4_9
ER  - 

@conference{
author = "Pešić, Mirjana and Barać, Miroljub and Stanojević, Sladjana and Vrvić, Miroslav M.",
year = "2016",
publisher = "Springer, New York",
journal = "Emerging and Traditional Technologies for Safe, Healthy and Quality Food",
title = "Heat-Induced Casein-Whey Protein Interactions in Caprine Milk: Whether Are Similar to Bovine Milk?",
pages = "175-163",
doi = "10.1007/978-3-319-24040-4_9"
}

Pešić, M., Barać, M., Stanojević, S.,& Vrvić, M. M.. (2016). Heat-Induced Casein-Whey Protein Interactions in Caprine Milk: Whether Are Similar to Bovine Milk?. in Emerging and Traditional Technologies for Safe, Healthy and Quality Food
Springer, New York., 163-175.
https://doi.org/10.1007/978-3-319-24040-4_9

Pešić M, Barać M, Stanojević S, Vrvić MM. Heat-Induced Casein-Whey Protein Interactions in Caprine Milk: Whether Are Similar to Bovine Milk?. in Emerging and Traditional Technologies for Safe, Healthy and Quality Food. 2016;:163-175.
doi:10.1007/978-3-319-24040-4_9 .

Pešić, Mirjana, Barać, Miroljub, Stanojević, Sladjana, Vrvić, Miroslav M., "Heat-Induced Casein-Whey Protein Interactions in Caprine Milk: Whether Are Similar to Bovine Milk?" in Emerging and Traditional Technologies for Safe, Healthy and Quality Food (2016):163-175,
https://doi.org/10.1007/978-3-319-24040-4_9 . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav M.
AU  - van Griensven, Leo
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3744
AB  - Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.
PB  - MDPI, BASEL
T2  - Molecules
T1  - Antioxidants of Edible Mushrooms
EP  - 19525
IS  - 10
SP  - 19489
VL  - 20
DO  - 10.3390/molecules201019489
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Vunduk, Jovana and Petrović, Predrag and Nikšić, Miomir and Vrvić, Miroslav M. and van Griensven, Leo",
year = "2015",
abstract = "Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.",
publisher = "MDPI, BASEL",
journal = "Molecules",
title = "Antioxidants of Edible Mushrooms",
pages = "19525-19489",
number = "10",
volume = "20",
doi = "10.3390/molecules201019489"
}

Kozarski, M., Klaus, A., Jakovljević, D., Todorović, N., Vunduk, J., Petrović, P., Nikšić, M., Vrvić, M. M.,& van Griensven, L.. (2015). Antioxidants of Edible Mushrooms. in Molecules
MDPI, BASEL., 20(10), 19489-19525.
https://doi.org/10.3390/molecules201019489

Kozarski M, Klaus A, Jakovljević D, Todorović N, Vunduk J, Petrović P, Nikšić M, Vrvić MM, van Griensven L. Antioxidants of Edible Mushrooms. in Molecules. 2015;20(10):19489-19525.
doi:10.3390/molecules201019489 .

Kozarski, Maja, Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Vunduk, Jovana, Petrović, Predrag, Nikšić, Miomir, Vrvić, Miroslav M., van Griensven, Leo, "Antioxidants of Edible Mushrooms" in Molecules, 20, no. 10 (2015):19489-19525,
https://doi.org/10.3390/molecules201019489 . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Zizak, Zeljko
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav M.
AU  - van Griensven, Leo
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3834
AB  - The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
EP  - 1886
IS  - 6
SP  - 1875
VL  - 6
DO  - 10.1039/c5fo00312a
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Vunduk, Jovana and Zizak, Zeljko and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav M. and van Griensven, Leo",
year = "2015",
abstract = "The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms",
pages = "1886-1875",
number = "6",
volume = "6",
doi = "10.1039/c5fo00312a"
}

Kozarski, M., Klaus, A., Vunduk, J., Zizak, Z., Nikšić, M., Jakovljević, D., Vrvić, M. M.,& van Griensven, L.. (2015). Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function
Royal Soc Chemistry, Cambridge., 6(6), 1875-1886.
https://doi.org/10.1039/c5fo00312a

Kozarski M, Klaus A, Vunduk J, Zizak Z, Nikšić M, Jakovljević D, Vrvić MM, van Griensven L. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function. 2015;6(6):1875-1886.
doi:10.1039/c5fo00312a .

Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Zizak, Zeljko, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav M., van Griensven, Leo, "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms" in Food & Function, 6, no. 6 (2015):1875-1886,
https://doi.org/10.1039/c5fo00312a . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav M.
AU  - van Griensven, Leo
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3451
AB  - Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification
EP  - 64
SP  - 53
VL  - 64
DO  - 10.1016/j.foodres.2014.05.075
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Nikšić, Miomir and Vrvić, Miroslav M. and van Griensven, Leo",
year = "2014",
abstract = "Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification",
pages = "64-53",
volume = "64",
doi = "10.1016/j.foodres.2014.05.075"
}

Kozarski, M., Klaus, A., Jakovljević, D., Todorović, N., Nikšić, M., Vrvić, M. M.,& van Griensven, L.. (2014). Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International
Elsevier Science Bv, Amsterdam., 64, 53-64.
https://doi.org/10.1016/j.foodres.2014.05.075

Kozarski M, Klaus A, Jakovljević D, Todorović N, Nikšić M, Vrvić MM, van Griensven L. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International. 2014;64:53-64.
doi:10.1016/j.foodres.2014.05.075 .

Kozarski, Maja, Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Nikšić, Miomir, Vrvić, Miroslav M., van Griensven, Leo, "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification" in Food Research International, 64 (2014):53-64,
https://doi.org/10.1016/j.foodres.2014.05.075 . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - van Griensven, Leo
AU  - Vrvić, Miroslav M.
AU  - Jakovljević, Dragica
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3626
AB  - The fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic, immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties.
AB  - Polisaharidi gljiva privlače veliku pažnju zbog svojih interesantnih bioloskih svojstava kao sto su: antitumorno, antivirusno, antikomplementarno, antikoagulaciono, hipolipidemijsko dejstvo, kao i imunomodulatorska i imunostimulativna aktivnost, sto ih sve čini pogodnim za primenu u mnogim oblastima, među kojima su i prehrambena industrija, kozmetika, biomedicina, poljoprivreda, zastita životne sredine i otpadnih voda. Oksidativna ostecenja prirodnih i industrijski pripremljenih namirnica predstavljaju veliki ekonomski problem na globalnom nivou, s obzirom da direktno utiču na promenu organoleptičkih osobina proizvoda, pri čemu dolazi i do stvaranja potencijalno toksičnih jedinjenja. Zbog toga se danas sve veća pažnja posvećuje istraživanjima novih prirodnih izvora antioksidanasa, među kojima su veoma značajni i polisaharidni ekstrakti različitih bazidiomiceta. U ovom radu su prikazana bioloska svojstva, struktura, kao i postupci izolovanja i aktivacije polisaharida visih gljiva. Dat je i pregled antioksidativne aktivnosti polisaharidnih ekstrakata visih gljiva i razmatran je uticaj strukturnih karakteristika ovih ekstrakata na antioksidativna svojstva.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Polysaccharides of higher fungi: Biological role, structure and antioxidative activity
T1  - Polisaharidi viših gljiva - bioloska uloga, struktura i antioksidativna aktivnost
EP  - 320
IS  - 3
SP  - 305
VL  - 68
DO  - 10.2298/HEMIND121114056K
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Nikšić, Miomir and van Griensven, Leo and Vrvić, Miroslav M. and Jakovljević, Dragica",
year = "2014",
abstract = "The fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic, immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties., Polisaharidi gljiva privlače veliku pažnju zbog svojih interesantnih bioloskih svojstava kao sto su: antitumorno, antivirusno, antikomplementarno, antikoagulaciono, hipolipidemijsko dejstvo, kao i imunomodulatorska i imunostimulativna aktivnost, sto ih sve čini pogodnim za primenu u mnogim oblastima, među kojima su i prehrambena industrija, kozmetika, biomedicina, poljoprivreda, zastita životne sredine i otpadnih voda. Oksidativna ostecenja prirodnih i industrijski pripremljenih namirnica predstavljaju veliki ekonomski problem na globalnom nivou, s obzirom da direktno utiču na promenu organoleptičkih osobina proizvoda, pri čemu dolazi i do stvaranja potencijalno toksičnih jedinjenja. Zbog toga se danas sve veća pažnja posvećuje istraživanjima novih prirodnih izvora antioksidanasa, među kojima su veoma značajni i polisaharidni ekstrakti različitih bazidiomiceta. U ovom radu su prikazana bioloska svojstva, struktura, kao i postupci izolovanja i aktivacije polisaharida visih gljiva. Dat je i pregled antioksidativne aktivnosti polisaharidnih ekstrakata visih gljiva i razmatran je uticaj strukturnih karakteristika ovih ekstrakata na antioksidativna svojstva.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Polysaccharides of higher fungi: Biological role, structure and antioxidative activity, Polisaharidi viših gljiva - bioloska uloga, struktura i antioksidativna aktivnost",
pages = "320-305",
number = "3",
volume = "68",
doi = "10.2298/HEMIND121114056K"
}

Kozarski, M., Klaus, A., Nikšić, M., van Griensven, L., Vrvić, M. M.,& Jakovljević, D.. (2014). Polysaccharides of higher fungi: Biological role, structure and antioxidative activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 68(3), 305-320.
https://doi.org/10.2298/HEMIND121114056K

Kozarski M, Klaus A, Nikšić M, van Griensven L, Vrvić MM, Jakovljević D. Polysaccharides of higher fungi: Biological role, structure and antioxidative activity. in Hemijska industrija. 2014;68(3):305-320.
doi:10.2298/HEMIND121114056K .

Kozarski, Maja, Klaus, Anita, Nikšić, Miomir, van Griensven, Leo, Vrvić, Miroslav M., Jakovljević, Dragica, "Polysaccharides of higher fungi: Biological role, structure and antioxidative activity" in Hemijska industrija, 68, no. 3 (2014):305-320,
https://doi.org/10.2298/HEMIND121114056K . .

TY  - JOUR
AU  - Pešić, Mirjana
AU  - Barać, Miroljub
AU  - Stanojević, Sladjana
AU  - Vrvić, Miroslav M.
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3396
AB  - Skim caprine and bovine milk, adjusted to pH 6.5-7.1 were heated at 90 degrees C for 10 min. The distribution of caseins, alpha-lactalbumin (alpha-LA) and beta-lactoglobulin (beta-LG) between micellar and serum phases of both types of milk were determined. Depending on milk pH, the involvement of kappa-casein (kappa-CN) ranged from similar to 8 to 52% and from similar to 65 to 70% of total kappa-CN in bovine and caprine milk, respectively. alpha(S2)-Casein and beta-casein were only a part of micelle-bound complexes at all pH values in caprine milk. Denatured bovine beta-LG and alpha-LA were involved in both soluble and micelle-bound heat-induced protein complexes. Conversely, denatured caprine beta-LG was mainly associated with casein micelles (98-86% at pH 6.5-7.1) and was found in soluble complexes at higher pH (6-12% at pH 6.9-7.1). All denatured caprine alpha-LA was micelle-bound at all pH values. This knowledge could be very useful for understanding, controlling and modification of technological-functional properties of caprine milk.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Dairy Journal
T1  - Effect of pH on heat-induced casein-whey protein interactions: A comparison between caprine milk and bovine milk
EP  - 183
IS  - 1
SP  - 178
VL  - 39
DO  - 10.1016/j.idairyj.2014.06.006
ER  - 

@article{
author = "Pešić, Mirjana and Barać, Miroljub and Stanojević, Sladjana and Vrvić, Miroslav M.",
year = "2014",
abstract = "Skim caprine and bovine milk, adjusted to pH 6.5-7.1 were heated at 90 degrees C for 10 min. The distribution of caseins, alpha-lactalbumin (alpha-LA) and beta-lactoglobulin (beta-LG) between micellar and serum phases of both types of milk were determined. Depending on milk pH, the involvement of kappa-casein (kappa-CN) ranged from similar to 8 to 52% and from similar to 65 to 70% of total kappa-CN in bovine and caprine milk, respectively. alpha(S2)-Casein and beta-casein were only a part of micelle-bound complexes at all pH values in caprine milk. Denatured bovine beta-LG and alpha-LA were involved in both soluble and micelle-bound heat-induced protein complexes. Conversely, denatured caprine beta-LG was mainly associated with casein micelles (98-86% at pH 6.5-7.1) and was found in soluble complexes at higher pH (6-12% at pH 6.9-7.1). All denatured caprine alpha-LA was micelle-bound at all pH values. This knowledge could be very useful for understanding, controlling and modification of technological-functional properties of caprine milk.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Dairy Journal",
title = "Effect of pH on heat-induced casein-whey protein interactions: A comparison between caprine milk and bovine milk",
pages = "183-178",
number = "1",
volume = "39",
doi = "10.1016/j.idairyj.2014.06.006"
}

Pešić, M., Barać, M., Stanojević, S.,& Vrvić, M. M.. (2014). Effect of pH on heat-induced casein-whey protein interactions: A comparison between caprine milk and bovine milk. in International Dairy Journal
Elsevier Sci Ltd, Oxford., 39(1), 178-183.
https://doi.org/10.1016/j.idairyj.2014.06.006

Pešić M, Barać M, Stanojević S, Vrvić MM. Effect of pH on heat-induced casein-whey protein interactions: A comparison between caprine milk and bovine milk. in International Dairy Journal. 2014;39(1):178-183.
doi:10.1016/j.idairyj.2014.06.006 .

Pešić, Mirjana, Barać, Miroljub, Stanojević, Sladjana, Vrvić, Miroslav M., "Effect of pH on heat-induced casein-whey protein interactions: A comparison between caprine milk and bovine milk" in International Dairy Journal, 39, no. 1 (2014):178-183,
https://doi.org/10.1016/j.idairyj.2014.06.006 . .

TY  - JOUR
AU  - Ramadan, Ali Mohamed Muftah
AU  - Knudsen-Šolević, Tatjana
AU  - Antić, Mališa
AU  - Beskoski, Vladimir
AU  - Vrvić, Miroslav M.
AU  - Schwarzbauer, Jan
AU  - Jovančićević, Branimir
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3329
AB  - It is well known that during biodegradation of oil under natural geological conditions, or oil pollutants in the environment, degradation of hydrocarbons occurs according to a well-defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation (non-stimulated bioremediation, without addition of biomass, nutrient substances and biosurfactant) of soil contaminated with heavy residual fuel oil (mazut) was conducted during a period of 6 months. Low abundance of n-alkanes in the fraction of total saturated hydrocarbons in the initial sample (identification was possible only after concentration by the urea adduction technique) showed that the investigated oil pollutant was at the boundary between the third and the fourth biodegradation level. During the experiment, an intense degradation of phenanthrene and its methyl-, dimethyl- and trimethyl-isomers was not accompanied by the removal of the remaining n-alkanes. The abundance of n-alkanes remained at the initial low level, even at end of the experiment when the pollutant reached one of the highest biodegradation levels. These results showed that the non-stimulated biodegradation of some hydrocarbons, despite their high biodegradability, had not proceeded completely to the end, even at final degradation stages. Under the condition of reduced availability of some hydrocarbons, microorganisms tend to opt for the less biodegradable but more accessible hydrocarbons.
AB  - Dobro je poznato da se u toku biodegradacije nafte u prirodnim geološkim uslovima, ili naftnog zagađivača u životnoj sredini, degradacija ugljovodonika odigrava prema definisanom rasporedu. Na primer, glavne promene u procesu razgradnje normalnih alkana dešavaju se u toku drugog, "blagog", i trećeg, "umerenog", stupnja (na skali biodegradacije od 1 do 10). Prema dosadašnjim istraživanjima, u četvrtom stupnju, kada počinju intenzivne promene fenantrena i njegovih metil izomera, normalni alkani su već u potpunosti uklonjeni. U ovom radu u toku perioda od 6 meseci izvođena je ex situ prirodna bioremedijacija (nestimulisana bioremedijacija bez dodatka biomase, hranljivih sastojaka i biosurfaktanata) zemljišta zagađenog mazutom. Niska obilnost normalnih alkana u frakciji ukupnih zasićenih alkana u početnom uzorku (identifikacija je bila mogućna tek nakon koncentrovanja pomoću karbamida) pokazala je da je ispitivani naftni zagađivač na granici između trećeg i četvrtog stupnja biodegradacije. Tokom eksperimenta, intenzivnu razgradnju fenantrena i njegovih metil, dimetil i trimetil izomera nije pratilo uklanjanje ostatka normalnih alkana. Njihova obilnost je ostala na početnom, niskom nivou i na kraju eksperimenta kada je zagađivač dostigao jedan od najviših stepena biodegradacije. Dobijeni rezultati su pokazali da se razgradnja pojedinih ugljovodonika uprkos njihovoj visokoj biodegradabilnosti ne odigrava do potpunog kraja ni u završnim fazama degradacije. U uslovima njihove smanjene dostupnosti, mikroorganizmi se opredeljuju za teže degradabilne, ali dostupnije ugljovodonike.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)
T1  - Degradabilnost normalnih alkana za vreme ex situ prirodne bioremedijacije zemljišta zagađenog mazutom
EP  - 1043
IS  - 7
SP  - 1035
VL  - 78
DO  - 10.2298/JSC120829106A
ER  - 

@article{
author = "Ramadan, Ali Mohamed Muftah and Knudsen-Šolević, Tatjana and Antić, Mališa and Beskoski, Vladimir and Vrvić, Miroslav M. and Schwarzbauer, Jan and Jovančićević, Branimir",
year = "2013",
abstract = "It is well known that during biodegradation of oil under natural geological conditions, or oil pollutants in the environment, degradation of hydrocarbons occurs according to a well-defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation (non-stimulated bioremediation, without addition of biomass, nutrient substances and biosurfactant) of soil contaminated with heavy residual fuel oil (mazut) was conducted during a period of 6 months. Low abundance of n-alkanes in the fraction of total saturated hydrocarbons in the initial sample (identification was possible only after concentration by the urea adduction technique) showed that the investigated oil pollutant was at the boundary between the third and the fourth biodegradation level. During the experiment, an intense degradation of phenanthrene and its methyl-, dimethyl- and trimethyl-isomers was not accompanied by the removal of the remaining n-alkanes. The abundance of n-alkanes remained at the initial low level, even at end of the experiment when the pollutant reached one of the highest biodegradation levels. These results showed that the non-stimulated biodegradation of some hydrocarbons, despite their high biodegradability, had not proceeded completely to the end, even at final degradation stages. Under the condition of reduced availability of some hydrocarbons, microorganisms tend to opt for the less biodegradable but more accessible hydrocarbons., Dobro je poznato da se u toku biodegradacije nafte u prirodnim geološkim uslovima, ili naftnog zagađivača u životnoj sredini, degradacija ugljovodonika odigrava prema definisanom rasporedu. Na primer, glavne promene u procesu razgradnje normalnih alkana dešavaju se u toku drugog, "blagog", i trećeg, "umerenog", stupnja (na skali biodegradacije od 1 do 10). Prema dosadašnjim istraživanjima, u četvrtom stupnju, kada počinju intenzivne promene fenantrena i njegovih metil izomera, normalni alkani su već u potpunosti uklonjeni. U ovom radu u toku perioda od 6 meseci izvođena je ex situ prirodna bioremedijacija (nestimulisana bioremedijacija bez dodatka biomase, hranljivih sastojaka i biosurfaktanata) zemljišta zagađenog mazutom. Niska obilnost normalnih alkana u frakciji ukupnih zasićenih alkana u početnom uzorku (identifikacija je bila mogućna tek nakon koncentrovanja pomoću karbamida) pokazala je da je ispitivani naftni zagađivač na granici između trećeg i četvrtog stupnja biodegradacije. Tokom eksperimenta, intenzivnu razgradnju fenantrena i njegovih metil, dimetil i trimetil izomera nije pratilo uklanjanje ostatka normalnih alkana. Njihova obilnost je ostala na početnom, niskom nivou i na kraju eksperimenta kada je zagađivač dostigao jedan od najviših stepena biodegradacije. Dobijeni rezultati su pokazali da se razgradnja pojedinih ugljovodonika uprkos njihovoj visokoj biodegradabilnosti ne odigrava do potpunog kraja ni u završnim fazama degradacije. U uslovima njihove smanjene dostupnosti, mikroorganizmi se opredeljuju za teže degradabilne, ali dostupnije ugljovodonike.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut), Degradabilnost normalnih alkana za vreme ex situ prirodne bioremedijacije zemljišta zagađenog mazutom",
pages = "1043-1035",
number = "7",
volume = "78",
doi = "10.2298/JSC120829106A"
}

Ramadan, A. M. M., Knudsen-Šolević, T., Antić, M., Beskoski, V., Vrvić, M. M., Schwarzbauer, J.,& Jovančićević, B.. (2013). Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut). in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 78(7), 1035-1043.
https://doi.org/10.2298/JSC120829106A

Ramadan AMM, Knudsen-Šolević T, Antić M, Beskoski V, Vrvić MM, Schwarzbauer J, Jovančićević B. Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut). in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2013;78(7):1035-1043.
doi:10.2298/JSC120829106A .

Ramadan, Ali Mohamed Muftah, Knudsen-Šolević, Tatjana, Antić, Mališa, Beskoski, Vladimir, Vrvić, Miroslav M., Schwarzbauer, Jan, Jovančićević, Branimir, "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 78, no. 7 (2013):1035-1043,
https://doi.org/10.2298/JSC120829106A . .

TY  - CONF
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav M.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2740
AB  - Fruiting bodies of some wild and cultivatable mushrooms contain medicinal compounds which are being used in traditional medicines and cosmetics. Champignon mushroom (Agaricus bisporus) is the most widely cultivated species of edible mushroom worldwide. This paper focuses on antioxidant and antimicrobial importance of A bisporus. Water-soluble polysaccharide-enriched fraction was isolated from the dry carpophores of Agaricus bisporus. Antioxidant activities were investigated using in vitro assay systems: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and chelating ability on ferrous ions. Antimicrobial activity was tested against Gram positive and Gram negative bacteria in vitro by disk diffusion method in order to determine the zones of inhibition. At concentrations of 0.1-10 mg/ml, the scavenging abilities of A bisporus ranged between 12.3-75.5 %. The radical scavenging ability of the positive controls-tocopherol and ascorbic acid, at the concentrations of 0.1-20 mg/ml, were between 79.9-80.8 and 80.6-91.1 %, respectively. Polysaccharide extract from A bisporus showed steadily increasing chelating ability as concentrations increased to 88.2 % at 20 mg/ml. The chelating ability of the citric acid was between 7.2-10.7 %, at the concentrations of 0.1-20 mg/ml. The study of antimicrobial potential of polysaccharide extract showed more potent activity against Gram-positive Enterococcus faecalis ATCC 49532 (26.7 ± 0.2 mm), Bacillus cereus 10876 (27.5 ± 0.4 mm), Geobacillus stearothermophylus ATCC 7953 (22.8 ± 0.3 mm) than Gram-negative bacteria Pseudomonas aeruginosa ATCC 35032 (10.4 ± 0.6 mm), Proteus hauseri ATCC 13315 (12.1 ± 0.1 mm) Escherichia coli (0157:H7) 35150 (12.7 ± 0.4 mm) with exception of Klebsiella pneumoniae ATCC 27736 (22.3 ± 0.2 mm).
PB  - 6th Central European Congress on Food, CEFood 2012
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Antioxidant and antimicrobial potentials of champignon mushroom
EP  - 149
SP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2740
ER  - 

@conference{
author = "Kozarski, Maja and Klaus, Anita and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav M.",
year = "2012",
abstract = "Fruiting bodies of some wild and cultivatable mushrooms contain medicinal compounds which are being used in traditional medicines and cosmetics. Champignon mushroom (Agaricus bisporus) is the most widely cultivated species of edible mushroom worldwide. This paper focuses on antioxidant and antimicrobial importance of A bisporus. Water-soluble polysaccharide-enriched fraction was isolated from the dry carpophores of Agaricus bisporus. Antioxidant activities were investigated using in vitro assay systems: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and chelating ability on ferrous ions. Antimicrobial activity was tested against Gram positive and Gram negative bacteria in vitro by disk diffusion method in order to determine the zones of inhibition. At concentrations of 0.1-10 mg/ml, the scavenging abilities of A bisporus ranged between 12.3-75.5 %. The radical scavenging ability of the positive controls-tocopherol and ascorbic acid, at the concentrations of 0.1-20 mg/ml, were between 79.9-80.8 and 80.6-91.1 %, respectively. Polysaccharide extract from A bisporus showed steadily increasing chelating ability as concentrations increased to 88.2 % at 20 mg/ml. The chelating ability of the citric acid was between 7.2-10.7 %, at the concentrations of 0.1-20 mg/ml. The study of antimicrobial potential of polysaccharide extract showed more potent activity against Gram-positive Enterococcus faecalis ATCC 49532 (26.7 ± 0.2 mm), Bacillus cereus 10876 (27.5 ± 0.4 mm), Geobacillus stearothermophylus ATCC 7953 (22.8 ± 0.3 mm) than Gram-negative bacteria Pseudomonas aeruginosa ATCC 35032 (10.4 ± 0.6 mm), Proteus hauseri ATCC 13315 (12.1 ± 0.1 mm) Escherichia coli (0157:H7) 35150 (12.7 ± 0.4 mm) with exception of Klebsiella pneumoniae ATCC 27736 (22.3 ± 0.2 mm).",
publisher = "6th Central European Congress on Food, CEFood 2012",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Antioxidant and antimicrobial potentials of champignon mushroom",
pages = "149-144",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2740"
}

Kozarski, M., Klaus, A., Nikšić, M., Jakovljević, D.,& Vrvić, M. M.. (2012). Antioxidant and antimicrobial potentials of champignon mushroom. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
6th Central European Congress on Food, CEFood 2012., 144-149.
https://hdl.handle.net/21.15107/rcub_agrospace_2740

Kozarski M, Klaus A, Nikšić M, Jakovljević D, Vrvić MM. Antioxidant and antimicrobial potentials of champignon mushroom. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:144-149.
https://hdl.handle.net/21.15107/rcub_agrospace_2740 .

Kozarski, Maja, Klaus, Anita, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav M., "Antioxidant and antimicrobial potentials of champignon mushroom" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):144-149,
https://hdl.handle.net/21.15107/rcub_agrospace_2740 .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav M.
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - van Griensven, Leo
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2956
AB  - Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50  lt  0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor
EP  - 153
IS  - 1-2
SP  - 144
VL  - 26
DO  - 10.1016/j.jfca.2012.02.004
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Nikšić, Miomir and Vrvić, Miroslav M. and Todorović, Nina and Jakovljević, Dragica and van Griensven, Leo",
year = "2012",
abstract = "Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50  lt  0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor",
pages = "153-144",
number = "1-2",
volume = "26",
doi = "10.1016/j.jfca.2012.02.004"
}

Kozarski, M., Klaus, A., Nikšić, M., Vrvić, M. M., Todorović, N., Jakovljević, D.,& van Griensven, L.. (2012). Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 26(1-2), 144-153.
https://doi.org/10.1016/j.jfca.2012.02.004

Kozarski M, Klaus A, Nikšić M, Vrvić MM, Todorović N, Jakovljević D, van Griensven L. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. in Journal of Food Composition and Analysis. 2012;26(1-2):144-153.
doi:10.1016/j.jfca.2012.02.004 .

Kozarski, Maja, Klaus, Anita, Nikšić, Miomir, Vrvić, Miroslav M., Todorović, Nina, Jakovljević, Dragica, van Griensven, Leo, "Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor" in Journal of Food Composition and Analysis, 26, no. 1-2 (2012):144-153,
https://doi.org/10.1016/j.jfca.2012.02.004 . .

TY  - JOUR
AU  - Pešić, Mirjana
AU  - Barać, Miroljub
AU  - Stanojević, Sladjana
AU  - Ristić, Nikola
AU  - Maćej, Ognjen
AU  - Vrvić, Miroslav M.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2972
AB  - This paper is a study on the distribution of the denatured whey proteins and kappa-casein in soluble and micelle-bound complexes in heat treated caprine and bovine milk (90 degrees C, 10 min) at natural pH (6.71). Proteins were fractionated using fractionation technique based on renneting and were analysed by three electrophoretic techniques: native PAGE, SDS-PAGE under reducing and non-reducing conditions. Lower than 3% of the total beta-LGs remained stable after heat treatment of both milk species, but bovine alpha-LA was more heat stable than its counterpart in caprine milk (29.6% against 3.82%). Denatured caprine whey proteins (>95%) were part of micelle-bound complexes whereas soluble complexes were not observed. Conversely, about 30% of denatured bovine whey proteins were involved in soluble complexes. About 24.2% of total kappa-CN was included into complexes formed in heat-treated bovine milk whereas in heat-treated caprine milk this percentage is about three times higher. Caprine micelle-bound complexes, apart from whey proteins and kappa-casein included also beta-casein and alpha(s2)-casein, which were not found in their bovine counterparts. This knowledge could be very useful in understanding the differences in technological-functional properties of caprine and bovine milk and to enable better control of dairy processes.
PB  - Elsevier Science Bv, Amsterdam
T2  - Small Ruminant Research
T1  - Heat induced casein-whey protein interactions at natural pH of milk: A comparison between caprine and bovine milk
EP  - 86
IS  - 1-3
SP  - 77
VL  - 108
DO  - 10.1016/j.smallrumres.2012.06.013
ER  - 

@article{
author = "Pešić, Mirjana and Barać, Miroljub and Stanojević, Sladjana and Ristić, Nikola and Maćej, Ognjen and Vrvić, Miroslav M.",
year = "2012",
abstract = "This paper is a study on the distribution of the denatured whey proteins and kappa-casein in soluble and micelle-bound complexes in heat treated caprine and bovine milk (90 degrees C, 10 min) at natural pH (6.71). Proteins were fractionated using fractionation technique based on renneting and were analysed by three electrophoretic techniques: native PAGE, SDS-PAGE under reducing and non-reducing conditions. Lower than 3% of the total beta-LGs remained stable after heat treatment of both milk species, but bovine alpha-LA was more heat stable than its counterpart in caprine milk (29.6% against 3.82%). Denatured caprine whey proteins (>95%) were part of micelle-bound complexes whereas soluble complexes were not observed. Conversely, about 30% of denatured bovine whey proteins were involved in soluble complexes. About 24.2% of total kappa-CN was included into complexes formed in heat-treated bovine milk whereas in heat-treated caprine milk this percentage is about three times higher. Caprine micelle-bound complexes, apart from whey proteins and kappa-casein included also beta-casein and alpha(s2)-casein, which were not found in their bovine counterparts. This knowledge could be very useful in understanding the differences in technological-functional properties of caprine and bovine milk and to enable better control of dairy processes.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Small Ruminant Research",
title = "Heat induced casein-whey protein interactions at natural pH of milk: A comparison between caprine and bovine milk",
pages = "86-77",
number = "1-3",
volume = "108",
doi = "10.1016/j.smallrumres.2012.06.013"
}

Pešić, M., Barać, M., Stanojević, S., Ristić, N., Maćej, O.,& Vrvić, M. M.. (2012). Heat induced casein-whey protein interactions at natural pH of milk: A comparison between caprine and bovine milk. in Small Ruminant Research
Elsevier Science Bv, Amsterdam., 108(1-3), 77-86.
https://doi.org/10.1016/j.smallrumres.2012.06.013

Pešić M, Barać M, Stanojević S, Ristić N, Maćej O, Vrvić MM. Heat induced casein-whey protein interactions at natural pH of milk: A comparison between caprine and bovine milk. in Small Ruminant Research. 2012;108(1-3):77-86.
doi:10.1016/j.smallrumres.2012.06.013 .

Pešić, Mirjana, Barać, Miroljub, Stanojević, Sladjana, Ristić, Nikola, Maćej, Ognjen, Vrvić, Miroslav M., "Heat induced casein-whey protein interactions at natural pH of milk: A comparison between caprine and bovine milk" in Small Ruminant Research, 108, no. 1-3 (2012):77-86,
https://doi.org/10.1016/j.smallrumres.2012.06.013 . .

TY  - JOUR
AU  - Novaković, Milan
AU  - Ramadan, Muftah Mohamed Ali
AU  - Šolević-Knudsen, Tatjana
AU  - Antić, Mališa
AU  - Beskoski, Vladimir
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav M.
AU  - Jovančićević, Branimir
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2977
AB  - Phenanthrene and methyl-phenanthrenes are major aromatic pollutants originating in particular from fuel oil. Phenanthrene is usually degraded faster than methyl-phenanthrenes under geological and environmental conditions. Here, we report a preferential and accelerated biodegradation of methyl-phenanthrenes versus phenanthrene in soil contaminated by fuel oil. The polluted soil was mixed with sawdust and sand to form a homogenized biopile. The biopile was continuously sprayed with microbial consortia isolated from crude oil-contaminated soil and treated by biosurfactants and nutritive substances for biostimulation. During a 6-month bioremediation experiment, a steady increase in the relative abundance of phenanthrene compared to methyl-phenathrenes was observed by gas chromatography-mass spectrometry. The increase was the highest for trimethyl-phenanthrenes, with a phenanthrene/trimethyl-phenanthrenes ratio increasing from 0.42 to 2.45. By contrast, the control, non-stimulated samples showed a ratio decrease from 0.85 to 0.11. Moreover, the results showed that the level of degradability depends on the number of methyl groups.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Chemistry Letters
T1  - Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil
EP  - 294
IS  - 3
SP  - 287
VL  - 10
DO  - 10.1007/s10311-012-0354-6
ER  - 

@article{
author = "Novaković, Milan and Ramadan, Muftah Mohamed Ali and Šolević-Knudsen, Tatjana and Antić, Mališa and Beskoski, Vladimir and Gojgić-Cvijović, Gordana and Vrvić, Miroslav M. and Jovančićević, Branimir",
year = "2012",
abstract = "Phenanthrene and methyl-phenanthrenes are major aromatic pollutants originating in particular from fuel oil. Phenanthrene is usually degraded faster than methyl-phenanthrenes under geological and environmental conditions. Here, we report a preferential and accelerated biodegradation of methyl-phenanthrenes versus phenanthrene in soil contaminated by fuel oil. The polluted soil was mixed with sawdust and sand to form a homogenized biopile. The biopile was continuously sprayed with microbial consortia isolated from crude oil-contaminated soil and treated by biosurfactants and nutritive substances for biostimulation. During a 6-month bioremediation experiment, a steady increase in the relative abundance of phenanthrene compared to methyl-phenathrenes was observed by gas chromatography-mass spectrometry. The increase was the highest for trimethyl-phenanthrenes, with a phenanthrene/trimethyl-phenanthrenes ratio increasing from 0.42 to 2.45. By contrast, the control, non-stimulated samples showed a ratio decrease from 0.85 to 0.11. Moreover, the results showed that the level of degradability depends on the number of methyl groups.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Chemistry Letters",
title = "Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil",
pages = "294-287",
number = "3",
volume = "10",
doi = "10.1007/s10311-012-0354-6"
}

Novaković, M., Ramadan, M. M. A., Šolević-Knudsen, T., Antić, M., Beskoski, V., Gojgić-Cvijović, G., Vrvić, M. M.,& Jovančićević, B.. (2012). Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil. in Environmental Chemistry Letters
Springer Heidelberg, Heidelberg., 10(3), 287-294.
https://doi.org/10.1007/s10311-012-0354-6

Novaković M, Ramadan MMA, Šolević-Knudsen T, Antić M, Beskoski V, Gojgić-Cvijović G, Vrvić MM, Jovančićević B. Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil. in Environmental Chemistry Letters. 2012;10(3):287-294.
doi:10.1007/s10311-012-0354-6 .

Novaković, Milan, Ramadan, Muftah Mohamed Ali, Šolević-Knudsen, Tatjana, Antić, Mališa, Beskoski, Vladimir, Gojgić-Cvijović, Gordana, Vrvić, Miroslav M., Jovančićević, Branimir, "Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil" in Environmental Chemistry Letters, 10, no. 3 (2012):287-294,
https://doi.org/10.1007/s10311-012-0354-6 . .

TY  - JOUR
AU  - Ilić, Mila
AU  - Antić, Mališa
AU  - Antić, Vesna
AU  - Schwarzbauer, Jan
AU  - Vrvić, Miroslav M.
AU  - Jovančićević, Branimir
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2691
AB  - Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Panevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Chemistry Letters
T1  - Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation
EP  - 140
IS  - 1
SP  - 133
VL  - 9
DO  - 10.1007/s10311-009-0257-3
ER  - 

@article{
author = "Ilić, Mila and Antić, Mališa and Antić, Vesna and Schwarzbauer, Jan and Vrvić, Miroslav M. and Jovančićević, Branimir",
year = "2011",
abstract = "Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Panevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Chemistry Letters",
title = "Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation",
pages = "140-133",
number = "1",
volume = "9",
doi = "10.1007/s10311-009-0257-3"
}

Ilić, M., Antić, M., Antić, V., Schwarzbauer, J., Vrvić, M. M.,& Jovančićević, B.. (2011). Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. in Environmental Chemistry Letters
Springer Heidelberg, Heidelberg., 9(1), 133-140.
https://doi.org/10.1007/s10311-009-0257-3

Ilić M, Antić M, Antić V, Schwarzbauer J, Vrvić MM, Jovančićević B. Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. in Environmental Chemistry Letters. 2011;9(1):133-140.
doi:10.1007/s10311-009-0257-3 .

Ilić, Mila, Antić, Mališa, Antić, Vesna, Schwarzbauer, Jan, Vrvić, Miroslav M., Jovančićević, Branimir, "Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation" in Environmental Chemistry Letters, 9, no. 1 (2011):133-140,
https://doi.org/10.1007/s10311-009-0257-3 . .

TY  - JOUR
AU  - Pešić, Mirjana
AU  - Barać, Miroljub
AU  - Vrvić, Miroslav M.
AU  - Ristić, Nikola
AU  - Maćej, Ognjen
AU  - Stanojević, Sladjana
AU  - Kostić, Aleksandar
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2677
AB  - The distributions of major whey proteins in acid wheys from different caprine/bovine and ovine/bovine milk mixtures were investigated using native-polyacrylamide gel electrophoresis (PAGE). Significantly different distributions of major whey proteins as individual proteins or as the sum of the same protein from different species were established. The caprine major whey proteins were dominant in mixtures with 10%, 20% and 30% bovine milk. The total beta-lactoglobulin to alpha-lactalbumin (beta-LGs/alpha-LAs) ratios ranged from 1.37 to 2.12 for caprine/bovine acid wheys. The corresponding ratios for ovine/bovine acid wheys were in the range 2.59-2.12. Linear relationships were estimated among the amounts of added bovine milk and the percentages of individual major whey proteins in all milk analysed, with square correlation coefficients from 0.990 to 0.997. These correlations enabled the use of native-PAGE as simple, reliable and low cost analytical method for determination the distributions of major whey proteins in caprine/bovine acid wheys.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Dairy Journal
T1  - The distributions of major whey proteins in acid wheys obtained from caprine/bovine and ovine/bovine milk mixtures
EP  - 838
IS  - 10
SP  - 831
VL  - 21
DO  - 10.1016/j.idairyj.2011.04.002
ER  - 

@article{
author = "Pešić, Mirjana and Barać, Miroljub and Vrvić, Miroslav M. and Ristić, Nikola and Maćej, Ognjen and Stanojević, Sladjana and Kostić, Aleksandar",
year = "2011",
abstract = "The distributions of major whey proteins in acid wheys from different caprine/bovine and ovine/bovine milk mixtures were investigated using native-polyacrylamide gel electrophoresis (PAGE). Significantly different distributions of major whey proteins as individual proteins or as the sum of the same protein from different species were established. The caprine major whey proteins were dominant in mixtures with 10%, 20% and 30% bovine milk. The total beta-lactoglobulin to alpha-lactalbumin (beta-LGs/alpha-LAs) ratios ranged from 1.37 to 2.12 for caprine/bovine acid wheys. The corresponding ratios for ovine/bovine acid wheys were in the range 2.59-2.12. Linear relationships were estimated among the amounts of added bovine milk and the percentages of individual major whey proteins in all milk analysed, with square correlation coefficients from 0.990 to 0.997. These correlations enabled the use of native-PAGE as simple, reliable and low cost analytical method for determination the distributions of major whey proteins in caprine/bovine acid wheys.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Dairy Journal",
title = "The distributions of major whey proteins in acid wheys obtained from caprine/bovine and ovine/bovine milk mixtures",
pages = "838-831",
number = "10",
volume = "21",
doi = "10.1016/j.idairyj.2011.04.002"
}

Pešić, M., Barać, M., Vrvić, M. M., Ristić, N., Maćej, O., Stanojević, S.,& Kostić, A.. (2011). The distributions of major whey proteins in acid wheys obtained from caprine/bovine and ovine/bovine milk mixtures. in International Dairy Journal
Elsevier Sci Ltd, Oxford., 21(10), 831-838.
https://doi.org/10.1016/j.idairyj.2011.04.002

Pešić M, Barać M, Vrvić MM, Ristić N, Maćej O, Stanojević S, Kostić A. The distributions of major whey proteins in acid wheys obtained from caprine/bovine and ovine/bovine milk mixtures. in International Dairy Journal. 2011;21(10):831-838.
doi:10.1016/j.idairyj.2011.04.002 .

Pešić, Mirjana, Barać, Miroljub, Vrvić, Miroslav M., Ristić, Nikola, Maćej, Ognjen, Stanojević, Sladjana, Kostić, Aleksandar, "The distributions of major whey proteins in acid wheys obtained from caprine/bovine and ovine/bovine milk mixtures" in International Dairy Journal, 21, no. 10 (2011):831-838,
https://doi.org/10.1016/j.idairyj.2011.04.002 . .

TY  - JOUR
AU  - Pešić, Mirjana
AU  - Barać, Miroljub
AU  - Vrvić, Miroslav M.
AU  - Ristić, Nikola
AU  - Maćej, Ognjen
AU  - Stanojević, Sladjana
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2582
AB  - Native-PAGE (polyacrylamide gel electrophoresis) was used for the simultaneous qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milk using whole milk samples as well as their whey protein fraction Quantification was based on measuring band intensity of bovine beta-lactoglobulins in all milk mixtures and bovine alpha-lactalbumin in caprine/bovine milk blends Linear relationships were established between the band intensity of bovine beta-lactoglobulins and alpha-lactalbumin vs volume percentage of added bovine milk in all milk analysed with the correlation coefficient from 0 9950 to 0 9998 These correlations enabling the quantification of bovine milk percentage within the wide range from 3% or 5% to 90% in caprine/bovine and ovine/bovine milk blends respectively The differences between the actual percentages of bovine milk present in the adulterated milk samples and those calculated using the regression lines were less than or equal to 5% for all samples This method offers a rapid determination combined with unequivocal identification of the bovine whey proteins in almost every caprine/bovine or ovine/bovine milk mixtures
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milks using native-PAGE
EP  - 1449
IS  - 4
SP  - 1443
VL  - 125
DO  - 10.1016/j.foodchem.2010.10.045
ER  - 

@article{
author = "Pešić, Mirjana and Barać, Miroljub and Vrvić, Miroslav M. and Ristić, Nikola and Maćej, Ognjen and Stanojević, Sladjana",
year = "2011",
abstract = "Native-PAGE (polyacrylamide gel electrophoresis) was used for the simultaneous qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milk using whole milk samples as well as their whey protein fraction Quantification was based on measuring band intensity of bovine beta-lactoglobulins in all milk mixtures and bovine alpha-lactalbumin in caprine/bovine milk blends Linear relationships were established between the band intensity of bovine beta-lactoglobulins and alpha-lactalbumin vs volume percentage of added bovine milk in all milk analysed with the correlation coefficient from 0 9950 to 0 9998 These correlations enabling the quantification of bovine milk percentage within the wide range from 3% or 5% to 90% in caprine/bovine and ovine/bovine milk blends respectively The differences between the actual percentages of bovine milk present in the adulterated milk samples and those calculated using the regression lines were less than or equal to 5% for all samples This method offers a rapid determination combined with unequivocal identification of the bovine whey proteins in almost every caprine/bovine or ovine/bovine milk mixtures",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milks using native-PAGE",
pages = "1449-1443",
number = "4",
volume = "125",
doi = "10.1016/j.foodchem.2010.10.045"
}

Pešić, M., Barać, M., Vrvić, M. M., Ristić, N., Maćej, O.,& Stanojević, S.. (2011). Qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milks using native-PAGE. in Food Chemistry
Elsevier Sci Ltd, Oxford., 125(4), 1443-1449.
https://doi.org/10.1016/j.foodchem.2010.10.045

Pešić M, Barać M, Vrvić MM, Ristić N, Maćej O, Stanojević S. Qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milks using native-PAGE. in Food Chemistry. 2011;125(4):1443-1449.
doi:10.1016/j.foodchem.2010.10.045 .

Pešić, Mirjana, Barać, Miroljub, Vrvić, Miroslav M., Ristić, Nikola, Maćej, Ognjen, Stanojević, Sladjana, "Qualitative and quantitative analysis of bovine milk adulteration in caprine and ovine milks using native-PAGE" in Food Chemistry, 125, no. 4 (2011):1443-1449,
https://doi.org/10.1016/j.foodchem.2010.10.045 . .

TY  - JOUR
AU  - Solević, Tatjana
AU  - Novaković, Milan
AU  - Ilić, Mila
AU  - Antić, Mališa
AU  - Vrvić, Miroslav M.
AU  - Jovančićević, Branimir
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2720
AB  - The bioremediation potential of the aerobic zymogenous microorganisms in soil (Danube alluvium, Pancevo, Serbia) for crude oil biodegradation was investigated. A mixture of paraffinic types of oils was used as the substrate. The laboratory experiment of the simulated oil biodegradation lasted 15, 30, 45, 60 and 75 days. In parallel, an experiment with a control sample was conducted. Extracts were isolated from the samples with chloroform in a separation funnel. From these extracts, the hydrocarbons were isolated by column chromatography and analyzed by gas chromatography mass spectrometry (GC MS). n-Alkanes, isoprenoids, phenanthrene and its derivatives with one and two methyl groups were quantitatively analyzed. The ability and efficiency of zymogenous microorganisms in soil for crude oil bioremediation was assessed by comparison between the composition of samples which were exposed to the microorganisms and the control sample. The investigated microorganisms showed the highest bioremediation potential in the biodegradation of n-alkanes and isoprenoids. A considerably high bioremediation potential was confirmed in the biodegradation of phenanthrene and methyl phenanthrenes. Low bioremediation potential of these microorganisms was proven in the case of polycyclic alkanes of the sterane and triterpane types and dimethyl phenanthrenes.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation
EP  - 438
IS  - 3
SP  - 425
VL  - 76
DO  - 10.2298/JSC100531033S
ER  - 

@article{
author = "Solević, Tatjana and Novaković, Milan and Ilić, Mila and Antić, Mališa and Vrvić, Miroslav M. and Jovančićević, Branimir",
year = "2011",
abstract = "The bioremediation potential of the aerobic zymogenous microorganisms in soil (Danube alluvium, Pancevo, Serbia) for crude oil biodegradation was investigated. A mixture of paraffinic types of oils was used as the substrate. The laboratory experiment of the simulated oil biodegradation lasted 15, 30, 45, 60 and 75 days. In parallel, an experiment with a control sample was conducted. Extracts were isolated from the samples with chloroform in a separation funnel. From these extracts, the hydrocarbons were isolated by column chromatography and analyzed by gas chromatography mass spectrometry (GC MS). n-Alkanes, isoprenoids, phenanthrene and its derivatives with one and two methyl groups were quantitatively analyzed. The ability and efficiency of zymogenous microorganisms in soil for crude oil bioremediation was assessed by comparison between the composition of samples which were exposed to the microorganisms and the control sample. The investigated microorganisms showed the highest bioremediation potential in the biodegradation of n-alkanes and isoprenoids. A considerably high bioremediation potential was confirmed in the biodegradation of phenanthrene and methyl phenanthrenes. Low bioremediation potential of these microorganisms was proven in the case of polycyclic alkanes of the sterane and triterpane types and dimethyl phenanthrenes.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation",
pages = "438-425",
number = "3",
volume = "76",
doi = "10.2298/JSC100531033S"
}

Solević, T., Novaković, M., Ilić, M., Antić, M., Vrvić, M. M.,& Jovančićević, B.. (2011). Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 76(3), 425-438.
https://doi.org/10.2298/JSC100531033S

Solević T, Novaković M, Ilić M, Antić M, Vrvić MM, Jovančićević B. Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2011;76(3):425-438.
doi:10.2298/JSC100531033S .

Solević, Tatjana, Novaković, Milan, Ilić, Mila, Antić, Mališa, Vrvić, Miroslav M., Jovančićević, Branimir, "Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 76, no. 3 (2011):425-438,
https://doi.org/10.2298/JSC100531033S . .

TY  - CONF
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
AU  - Antić, Mališa
AU  - Solević, Tatjana
AU  - Ilić, Mila
AU  - Novaković, Milan
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2035
PB  - Amer Chemical Soc, Washington
C3  - Abstracts of Papers of the American Chemical Society
T1  - ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil
VL  - 238
UR  - https://hdl.handle.net/21.15107/rcub_cherry_524
ER  - 

@conference{
author = "Jovančićević, Branimir and Vrvić, Miroslav M. and Antić, Mališa and Solević, Tatjana and Ilić, Mila and Novaković, Milan",
year = "2009",
publisher = "Amer Chemical Soc, Washington",
journal = "Abstracts of Papers of the American Chemical Society",
title = "ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil",
volume = "238",
url = "https://hdl.handle.net/21.15107/rcub_cherry_524"
}

Jovančićević, B., Vrvić, M. M., Antić, M., Solević, T., Ilić, M.,& Novaković, M.. (2009). ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in Abstracts of Papers of the American Chemical Society
Amer Chemical Soc, Washington., 238.
https://hdl.handle.net/21.15107/rcub_cherry_524

Jovančićević B, Vrvić MM, Antić M, Solević T, Ilić M, Novaković M. ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in Abstracts of Papers of the American Chemical Society. 2009;238.
https://hdl.handle.net/21.15107/rcub_cherry_524 .

Jovančićević, Branimir, Vrvić, Miroslav M., Antić, Mališa, Solević, Tatjana, Ilić, Mila, Novaković, Milan, "ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil" in Abstracts of Papers of the American Chemical Society, 238 (2009),
https://hdl.handle.net/21.15107/rcub_cherry_524 .

TY  - CONF
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
AU  - Antić, Mališa
AU  - Solević, T.
AU  - Ilić, M.
AU  - Novaković, M.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1844
AB  - Bioremediation potential of bacteria and fungi isolated from sediment samples from an alluvial formation of the Danube (wastewater channel) of the Pancevo Industrial Zone (Serbia) was investigated earlier. (Ilic M., Antic M., Antic V., Schwarzbauer J., Vrvic M. and Jovancicevic B., Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. Environmental Chemistry Letters (submitted)) In this paper, results concerning bioremediation potential of bacteria and fungi isolated from soil samples will be presented. Total isolated microorganisms were divided into three parts and sown in an appropriate medium. Actidione fungicide was added to one innoculum so that only bacteria propagated in it. Streptomycin antibiotic was added to a second innoculum, wherein fungi propagated. The third portion (with no additives) contained consortium of fungi and bacteria. Paraffinic petroleum, in inorganic medium in phosphate puffer, was a substrate for assessment of bioremediation potential of the mentioned microorganisms. The experiments of the simulated oil biodegradation lasted 30, 60 and 90 days. Extracts were isolated from the samples with chloroform liquid-liquid extraction. Extracts were fractionated by column chromatography. Alkane fraction was analyzed by gas chromatography-mass spectrometry (GC-MS) techniques.
PB  - 238th National Meeting and Exposition of the American Chemical Society, ACS 2009
C3  - ACS National Meeting Book of Abstracts
T1  - Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil
UR  - https://hdl.handle.net/21.15107/rcub_cherry_111
ER  - 

@conference{
author = "Jovančićević, Branimir and Vrvić, Miroslav M. and Antić, Mališa and Solević, T. and Ilić, M. and Novaković, M.",
year = "2009",
abstract = "Bioremediation potential of bacteria and fungi isolated from sediment samples from an alluvial formation of the Danube (wastewater channel) of the Pancevo Industrial Zone (Serbia) was investigated earlier. (Ilic M., Antic M., Antic V., Schwarzbauer J., Vrvic M. and Jovancicevic B., Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. Environmental Chemistry Letters (submitted)) In this paper, results concerning bioremediation potential of bacteria and fungi isolated from soil samples will be presented. Total isolated microorganisms were divided into three parts and sown in an appropriate medium. Actidione fungicide was added to one innoculum so that only bacteria propagated in it. Streptomycin antibiotic was added to a second innoculum, wherein fungi propagated. The third portion (with no additives) contained consortium of fungi and bacteria. Paraffinic petroleum, in inorganic medium in phosphate puffer, was a substrate for assessment of bioremediation potential of the mentioned microorganisms. The experiments of the simulated oil biodegradation lasted 30, 60 and 90 days. Extracts were isolated from the samples with chloroform liquid-liquid extraction. Extracts were fractionated by column chromatography. Alkane fraction was analyzed by gas chromatography-mass spectrometry (GC-MS) techniques.",
publisher = "238th National Meeting and Exposition of the American Chemical Society, ACS 2009",
journal = "ACS National Meeting Book of Abstracts",
title = "Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil",
url = "https://hdl.handle.net/21.15107/rcub_cherry_111"
}

Jovančićević, B., Vrvić, M. M., Antić, M., Solević, T., Ilić, M.,& Novaković, M.. (2009). Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in ACS National Meeting Book of Abstracts
238th National Meeting and Exposition of the American Chemical Society, ACS 2009..
https://hdl.handle.net/21.15107/rcub_cherry_111

Jovančićević B, Vrvić MM, Antić M, Solević T, Ilić M, Novaković M. Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in ACS National Meeting Book of Abstracts. 2009;.
https://hdl.handle.net/21.15107/rcub_cherry_111 .

Jovančićević, Branimir, Vrvić, Miroslav M., Antić, Mališa, Solević, T., Ilić, M., Novaković, M., "Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil" in ACS National Meeting Book of Abstracts (2009),
https://hdl.handle.net/21.15107/rcub_cherry_111 .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Vrvić, Miroslav M.
AU  - Ilić, M.
AU  - Novaković, M.
AU  - Saheed, R.M.
AU  - Schwarzbauer, Jan
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1808
AB  - The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P-1-P-12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P-8-P-12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Transformation of a petroleum pollutant during soil bioremediation experiments
EP  - 583
IS  - 5
SP  - 577
VL  - 73
DO  - 10.2298/JSC0805577J
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Vrvić, Miroslav M. and Ilić, M. and Novaković, M. and Saheed, R.M. and Schwarzbauer, Jan",
year = "2008",
abstract = "The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P-1-P-12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P-8-P-12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Transformation of a petroleum pollutant during soil bioremediation experiments",
pages = "583-577",
number = "5",
volume = "73",
doi = "10.2298/JSC0805577J"
}

Jovančićević, B., Antić, M., Vrvić, M. M., Ilić, M., Novaković, M., Saheed, R.M.,& Schwarzbauer, J.. (2008). Transformation of a petroleum pollutant during soil bioremediation experiments. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 73(5), 577-583.
https://doi.org/10.2298/JSC0805577J

Jovančićević B, Antić M, Vrvić MM, Ilić M, Novaković M, Saheed R, Schwarzbauer J. Transformation of a petroleum pollutant during soil bioremediation experiments. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2008;73(5):577-583.
doi:10.2298/JSC0805577J .

Jovančićević, Branimir, Antić, Mališa, Vrvić, Miroslav M., Ilić, M., Novaković, M., Saheed, R.M., Schwarzbauer, Jan, "Transformation of a petroleum pollutant during soil bioremediation experiments" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 73, no. 5 (2008):577-583,
https://doi.org/10.2298/JSC0805577J . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Pavlović, I.
AU  - Vrvić, Miroslav M.
AU  - Beskoski, Vladimir
AU  - Kronimus, Alexander
AU  - Schwarzbauer, Jan
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1632
AB  - This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pančevo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P1-P12 samples). Organic substance was extracted by Soxhlet's method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P1. Pristane and phytane occurred in sample P8, and in even higher quantities in the final sample P12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P1 the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P8 and P12 because of the reduced concentration. The observed changes in the alkane fractions' compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.
T2  - Water, Air, and Soil Pollution
T1  - Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments
EP  - 307
IS  - 1-4
SP  - 299
VL  - 190
DO  - 10.1007/s11270-007-9601-z
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Pavlović, I. and Vrvić, Miroslav M. and Beskoski, Vladimir and Kronimus, Alexander and Schwarzbauer, Jan",
year = "2008",
abstract = "This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pančevo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P1-P12 samples). Organic substance was extracted by Soxhlet's method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P1. Pristane and phytane occurred in sample P8, and in even higher quantities in the final sample P12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P1 the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P8 and P12 because of the reduced concentration. The observed changes in the alkane fractions' compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.",
journal = "Water, Air, and Soil Pollution",
title = "Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments",
pages = "307-299",
number = "1-4",
volume = "190",
doi = "10.1007/s11270-007-9601-z"
}

Jovančićević, B., Antić, M., Pavlović, I., Vrvić, M. M., Beskoski, V., Kronimus, A.,& Schwarzbauer, J.. (2008). Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments. in Water, Air, and Soil Pollution, 190(1-4), 299-307.
https://doi.org/10.1007/s11270-007-9601-z

Jovančićević B, Antić M, Pavlović I, Vrvić MM, Beskoski V, Kronimus A, Schwarzbauer J. Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments. in Water, Air, and Soil Pollution. 2008;190(1-4):299-307.
doi:10.1007/s11270-007-9601-z .

Jovančićević, Branimir, Antić, Mališa, Pavlović, I., Vrvić, Miroslav M., Beskoski, Vladimir, Kronimus, Alexander, Schwarzbauer, Jan, "Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments" in Water, Air, and Soil Pollution, 190, no. 1-4 (2008):299-307,
https://doi.org/10.1007/s11270-007-9601-z . .

TY  - CONF
AU  - Ilić, Mila
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Ljaljević-Grbić, Milica
AU  - Cvijan, Mirko
AU  - Vrvić, Miroslav M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1597
PB  - Elsevier Science Bv, Amsterdam
C3  - Journal of Biotechnology
T1  - Surface water microorganisms degrade dominantly petroleum hydrocarbons
EP  - S150
IS  - 2
SP  - S150
VL  - 131
DO  - 10.1016/j.jbiotec.2007.07.865
ER  - 

@conference{
author = "Ilić, Mila and Jovančićević, Branimir and Antić, Mališa and Ljaljević-Grbić, Milica and Cvijan, Mirko and Vrvić, Miroslav M.",
year = "2007",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Surface water microorganisms degrade dominantly petroleum hydrocarbons",
pages = "S150-S150",
number = "2",
volume = "131",
doi = "10.1016/j.jbiotec.2007.07.865"
}

Ilić, M., Jovančićević, B., Antić, M., Ljaljević-Grbić, M., Cvijan, M.,& Vrvić, M. M.. (2007). Surface water microorganisms degrade dominantly petroleum hydrocarbons. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 131(2), S150-S150.
https://doi.org/10.1016/j.jbiotec.2007.07.865

Ilić M, Jovančićević B, Antić M, Ljaljević-Grbić M, Cvijan M, Vrvić MM. Surface water microorganisms degrade dominantly petroleum hydrocarbons. in Journal of Biotechnology. 2007;131(2):S150-S150.
doi:10.1016/j.jbiotec.2007.07.865 .

Ilić, Mila, Jovančićević, Branimir, Antić, Mališa, Ljaljević-Grbić, Milica, Cvijan, Mirko, Vrvić, Miroslav M., "Surface water microorganisms degrade dominantly petroleum hydrocarbons" in Journal of Biotechnology, 131, no. 2 (2007):S150-S150,
https://doi.org/10.1016/j.jbiotec.2007.07.865 . .

TY  - JOUR
AU  - Antić, Mališa
AU  - Jovančićević, Branimir
AU  - Ilić, Mila
AU  - Vrvić, Miroslav M.
AU  - Schwarzbauer, Jan
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1351
AB  - BACKGROUND: , Aims and Scope. It is well known that the composition of petroleum or some of its processing products changes in the environment mostly under the influence of microorganisms. A series of experiments was conducted in order to define the optimum conditions for an efficient biodegradation of petroleum pollutant, or bioremediation of different segments of the environment. The aim of these investigations was to show to what extent the hydrocarbons of a petroleum pollutant are degraded by microbial cultures which were isolated as dominant microorganisms from a surface water of a wastewater canal of an oil refinery and a nitrogen plant. Biodegradation experiments were conducted on one paraffinic, and one naphthenic type of petroleum during a three month period under aerobic conditions, varying the following parameters: Inorganic (Kp) or an organic medium (Bh) with or without exposition to light. Methods. Microorganisms were analyzed in a surface water sample from a canal (Pancevo, Serbia), into which wastewater from an oil refinery and a nitrogen plant is released. The consortia of microorganisms were isolated from the water sample (most abundant species: Phormidium foveolarum - filamentous Cyanobacteria, blue-green algae and Achanthes minutissima, diatoms, algae). The simulation experiments of biodegradation were conducted with the biomass suspension and crude oils Sirakovo (Sir, paraffinic type) and Velebit (Ve, naphthenic type). After a three month period, organic substance was extracted by means of chloroform. In the extracts, the content of saturated hydrocarbons, aromatic hydrocarbons, alcohols and fatty acids was determined (the group composition). n-Alkanes and isoprenoid aliphatic alkanes, pristane and phytane, in the aliphatic fractions, were analyzed using gas chromatography (GC). Total isoprenoid aliphatic alkanes and polycyclic alkanes of sterane and triterpane types were analyzed by GC-MS. Results and Discussion. Paraffinic type petroleums have a significant loss of saturated hydrocarbons. For naphthenic type petroleum, such a trend has not been observed. The most intensive degradation of n-alkanes and isoprenoid aliphatic alkanes (in paraffinic oil) and isoprenoids (in naphthenic oil) was observed using the inorganic medium Kp in the light; the microbial conversion is somewhat lower with Kp in the dark; with organic medium Bh in the light the degradation is of low intensity; with the same medium in the dark the degradation is hardly to be seen. Steranes and triterpanes were not affected by microbial degradation under the conditions used in our experiments. Obviously, the petroleum biodegradation was restricted to the acyclic aliphatics (n-alkanes and isoprenoids). Conclusion. Phormidium foveolarum (filamentous Cyanobacteria - blue-green algae) and Achanthes minutissima (diatoms, algae), microbial cultures isolated as dominant algae from a surface water in a wastewater canal of an oil refinery and a nitrogen plant, have degradable effects dominantly involving petroleum hydocarbons. Petroleum microbiological degradation is more intensive when inorganic medium (in the light) is applied. Having in mind that the inorganic pollutants have been released into the canal as well, this medium reflects more the natural environmental conditions. Polycyclic alkanes of sterane and triterpane type, in spite of the fact that these compounds could be degraded, have remained unchanged regarding abundance and distribution. Since this is the case even for naphthenic type petroleum (which is depleted in n-alkanes), it can be concluded that the biodegradation of petroleum type pollutants, under natural conditions, will be restrained to the n-alkane and isoprenoid degradation. Recommendation and Outlook. Performed experiments and simulations of petroleum microbiological degradation may serve for the prediction of the fate of petroleum type pollutants, as well as for definition of conditions for bioremediation of some environmental segments.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Petroleum pollutant degradation by surface water microorganisms
EP  - 327
IS  - 5
SP  - 320
VL  - 13
DO  - 10.1065/espr2006.03.296
ER  - 

@article{
author = "Antić, Mališa and Jovančićević, Branimir and Ilić, Mila and Vrvić, Miroslav M. and Schwarzbauer, Jan",
year = "2006",
abstract = "BACKGROUND: , Aims and Scope. It is well known that the composition of petroleum or some of its processing products changes in the environment mostly under the influence of microorganisms. A series of experiments was conducted in order to define the optimum conditions for an efficient biodegradation of petroleum pollutant, or bioremediation of different segments of the environment. The aim of these investigations was to show to what extent the hydrocarbons of a petroleum pollutant are degraded by microbial cultures which were isolated as dominant microorganisms from a surface water of a wastewater canal of an oil refinery and a nitrogen plant. Biodegradation experiments were conducted on one paraffinic, and one naphthenic type of petroleum during a three month period under aerobic conditions, varying the following parameters: Inorganic (Kp) or an organic medium (Bh) with or without exposition to light. Methods. Microorganisms were analyzed in a surface water sample from a canal (Pancevo, Serbia), into which wastewater from an oil refinery and a nitrogen plant is released. The consortia of microorganisms were isolated from the water sample (most abundant species: Phormidium foveolarum - filamentous Cyanobacteria, blue-green algae and Achanthes minutissima, diatoms, algae). The simulation experiments of biodegradation were conducted with the biomass suspension and crude oils Sirakovo (Sir, paraffinic type) and Velebit (Ve, naphthenic type). After a three month period, organic substance was extracted by means of chloroform. In the extracts, the content of saturated hydrocarbons, aromatic hydrocarbons, alcohols and fatty acids was determined (the group composition). n-Alkanes and isoprenoid aliphatic alkanes, pristane and phytane, in the aliphatic fractions, were analyzed using gas chromatography (GC). Total isoprenoid aliphatic alkanes and polycyclic alkanes of sterane and triterpane types were analyzed by GC-MS. Results and Discussion. Paraffinic type petroleums have a significant loss of saturated hydrocarbons. For naphthenic type petroleum, such a trend has not been observed. The most intensive degradation of n-alkanes and isoprenoid aliphatic alkanes (in paraffinic oil) and isoprenoids (in naphthenic oil) was observed using the inorganic medium Kp in the light; the microbial conversion is somewhat lower with Kp in the dark; with organic medium Bh in the light the degradation is of low intensity; with the same medium in the dark the degradation is hardly to be seen. Steranes and triterpanes were not affected by microbial degradation under the conditions used in our experiments. Obviously, the petroleum biodegradation was restricted to the acyclic aliphatics (n-alkanes and isoprenoids). Conclusion. Phormidium foveolarum (filamentous Cyanobacteria - blue-green algae) and Achanthes minutissima (diatoms, algae), microbial cultures isolated as dominant algae from a surface water in a wastewater canal of an oil refinery and a nitrogen plant, have degradable effects dominantly involving petroleum hydocarbons. Petroleum microbiological degradation is more intensive when inorganic medium (in the light) is applied. Having in mind that the inorganic pollutants have been released into the canal as well, this medium reflects more the natural environmental conditions. Polycyclic alkanes of sterane and triterpane type, in spite of the fact that these compounds could be degraded, have remained unchanged regarding abundance and distribution. Since this is the case even for naphthenic type petroleum (which is depleted in n-alkanes), it can be concluded that the biodegradation of petroleum type pollutants, under natural conditions, will be restrained to the n-alkane and isoprenoid degradation. Recommendation and Outlook. Performed experiments and simulations of petroleum microbiological degradation may serve for the prediction of the fate of petroleum type pollutants, as well as for definition of conditions for bioremediation of some environmental segments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Petroleum pollutant degradation by surface water microorganisms",
pages = "327-320",
number = "5",
volume = "13",
doi = "10.1065/espr2006.03.296"
}

Antić, M., Jovančićević, B., Ilić, M., Vrvić, M. M.,& Schwarzbauer, J.. (2006). Petroleum pollutant degradation by surface water microorganisms. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 13(5), 320-327.
https://doi.org/10.1065/espr2006.03.296

Antić M, Jovančićević B, Ilić M, Vrvić MM, Schwarzbauer J. Petroleum pollutant degradation by surface water microorganisms. in Environmental Science and Pollution Research. 2006;13(5):320-327.
doi:10.1065/espr2006.03.296 .

Antić, Mališa, Jovančićević, Branimir, Ilić, Mila, Vrvić, Miroslav M., Schwarzbauer, Jan, "Petroleum pollutant degradation by surface water microorganisms" in Environmental Science and Pollution Research, 13, no. 5 (2006):320-327,
https://doi.org/10.1065/espr2006.03.296 . .