TY  - CONF
AU  - Vujović, Bojana
AU  - Jovičić-Petrović, Jelena
AU  - Rašković, Nikola
AU  - Vukmanović, Tamara
AU  - Kljujev, Igor
AU  - Raičević, Vera
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6844
AB  - Podzemne vode predstavljaju najočuvaniji deo hidrosfere, zaštićen od globalne
degradacije kvaliteta vode, zbog čega je široko rasprostranjen i često korišćen resurs
vodosnabdevanja. Iako se podzemne vode smatraju mikrobiološki slabo naseljenim
delom biosfere, transformacija organskog ugljenika, transformacija azota, gvožđa i
sumpora između oksidovanog i redukovanog stanja, produkcija metana i drugi
fundamentalni procesi se i u podzemnim vodama odigravaju mikrobiološkom
aktivnošću.1 Iako bakterije koje pripadaju različitim fiziološkim grupama nisu od
primarnog značaja pri proceni pogodnosti vode za upotrebu, populacija "normalno" ili
"prirodno" prisutnih bakterija utiče na organoleptička svojstva, pH, redoks potencijal,
količinu kiseonika i druge karakteristike vode.1,2 U radu su prikazani rezultati
kvalitativne i kvantitativne zastupljenosti gvožđevitih, sulfat redukujućih, nitrifikujućih,
denitrifikujućih, fluorescirajućih i sluz produkujućih bakterija u vodi poreklom iz tri
bunara primenom BART testova (Biological Activity Reaction Tests). Takođe, u radu je
prikazan i broj ukupnih i fekalnih koliformnih bakterija određen metodom IDEXX
Colilert-18/Quanty-Tray 2000 i fekalnih enterokoka metodom Enterolert-E/Quanty-Tray
2000. Dobijeni rezultati ukazuju da postoji pozitivna korelacija između biodiverziteta
različitih grupa testiranih bakterija, što ukazuje na složene ekološke abiotičke i biotičke
odnose u podzemenim vodama i bunarima. Iako je autohtona bakterijska zajednica
uslovljena geološkim svojstvima vode, ove bakterije mogu uticati i limitirati upotrebu
podzemne vode u vodosnabdevanju stupanjem u odnose sa patogenim ili potencijalno
patogenim organizmima
C3  - knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo
T1  - Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6844
ER  - 

@conference{
author = "Vujović, Bojana and Jovičić-Petrović, Jelena and Rašković, Nikola and Vukmanović, Tamara and Kljujev, Igor and Raičević, Vera",
year = "2018",
abstract = "Podzemne vode predstavljaju najočuvaniji deo hidrosfere, zaštićen od globalne
degradacije kvaliteta vode, zbog čega je široko rasprostranjen i često korišćen resurs
vodosnabdevanja. Iako se podzemne vode smatraju mikrobiološki slabo naseljenim
delom biosfere, transformacija organskog ugljenika, transformacija azota, gvožđa i
sumpora između oksidovanog i redukovanog stanja, produkcija metana i drugi
fundamentalni procesi se i u podzemnim vodama odigravaju mikrobiološkom
aktivnošću.1 Iako bakterije koje pripadaju različitim fiziološkim grupama nisu od
primarnog značaja pri proceni pogodnosti vode za upotrebu, populacija "normalno" ili
"prirodno" prisutnih bakterija utiče na organoleptička svojstva, pH, redoks potencijal,
količinu kiseonika i druge karakteristike vode.1,2 U radu su prikazani rezultati
kvalitativne i kvantitativne zastupljenosti gvožđevitih, sulfat redukujućih, nitrifikujućih,
denitrifikujućih, fluorescirajućih i sluz produkujućih bakterija u vodi poreklom iz tri
bunara primenom BART testova (Biological Activity Reaction Tests). Takođe, u radu je
prikazan i broj ukupnih i fekalnih koliformnih bakterija određen metodom IDEXX
Colilert-18/Quanty-Tray 2000 i fekalnih enterokoka metodom Enterolert-E/Quanty-Tray
2000. Dobijeni rezultati ukazuju da postoji pozitivna korelacija između biodiverziteta
različitih grupa testiranih bakterija, što ukazuje na složene ekološke abiotičke i biotičke
odnose u podzemenim vodama i bunarima. Iako je autohtona bakterijska zajednica
uslovljena geološkim svojstvima vode, ove bakterije mogu uticati i limitirati upotrebu
podzemne vode u vodosnabdevanju stupanjem u odnose sa patogenim ili potencijalno
patogenim organizmima",
journal = "knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo",
title = "Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6844"
}

Vujović, B., Jovičić-Petrović, J., Rašković, N., Vukmanović, T., Kljujev, I.,& Raičević, V.. (2018). Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi. in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo.
https://hdl.handle.net/21.15107/rcub_agrospace_6844

Vujović B, Jovičić-Petrović J, Rašković N, Vukmanović T, Kljujev I, Raičević V. Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi. in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_6844 .

Vujović, Bojana, Jovičić-Petrović, Jelena, Rašković, Nikola, Vukmanović, Tamara, Kljujev, Igor, Raičević, Vera, "Primena BART testova u ispitivanju fiziološkog diverziteta bakterija u podzemnoj vodi" in knjiga apstrakata, Drugi kongres biologa Srbije. (p. 242). Kladovo (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_6844 .

TY  - JOUR
AU  - Rudić, Željka
AU  - Vujović, Bojana
AU  - Božić, Mile
AU  - Arizanović, Tanja
AU  - Raičević, Vera
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4621
AB  - The objective of this paper was to emphasise how faecal bacteria persist in canal sediment and relate abiotic factors in a pen-urban canal. Mann-Whitney tests confirmed that there is a considerable difference in water quality of upstream and downstream ends of the canal, which supports the claim that water quality is affected by wastewater discharge. Strong positive correlations between enterococci and organic matter, TOC and all nitrogen forms in sediment show that the large amount of organic matter in sediment is linked to high enterococcal counts. High counts of E. coli in sediment, as well as weak correlations between E. coli and sediment quality parameters, together with water quality changes, indicate a 'fresh' pollution of aquatic system. In conclusion, our study suggests that wastewater discharge makes a long-term contribution to the increased content of E. cob and enterococci in the Palic-Ludas canal.
PB  - Inderscience Enterprises Ltd, Geneva
T2  - International Journal of Environment and Pollution
T1  - Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia
EP  - 309
IS  - 4
SP  - 292
VL  - 64
DO  - 10.1504/IJEP.2018.099463
ER  - 

@article{
author = "Rudić, Željka and Vujović, Bojana and Božić, Mile and Arizanović, Tanja and Raičević, Vera",
year = "2018",
abstract = "The objective of this paper was to emphasise how faecal bacteria persist in canal sediment and relate abiotic factors in a pen-urban canal. Mann-Whitney tests confirmed that there is a considerable difference in water quality of upstream and downstream ends of the canal, which supports the claim that water quality is affected by wastewater discharge. Strong positive correlations between enterococci and organic matter, TOC and all nitrogen forms in sediment show that the large amount of organic matter in sediment is linked to high enterococcal counts. High counts of E. coli in sediment, as well as weak correlations between E. coli and sediment quality parameters, together with water quality changes, indicate a 'fresh' pollution of aquatic system. In conclusion, our study suggests that wastewater discharge makes a long-term contribution to the increased content of E. cob and enterococci in the Palic-Ludas canal.",
publisher = "Inderscience Enterprises Ltd, Geneva",
journal = "International Journal of Environment and Pollution",
title = "Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia",
pages = "309-292",
number = "4",
volume = "64",
doi = "10.1504/IJEP.2018.099463"
}

Rudić, Ž., Vujović, B., Božić, M., Arizanović, T.,& Raičević, V.. (2018). Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia. in International Journal of Environment and Pollution
Inderscience Enterprises Ltd, Geneva., 64(4), 292-309.
https://doi.org/10.1504/IJEP.2018.099463

Rudić Ž, Vujović B, Božić M, Arizanović T, Raičević V. Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia. in International Journal of Environment and Pollution. 2018;64(4):292-309.
doi:10.1504/IJEP.2018.099463 .

Rudić, Željka, Vujović, Bojana, Božić, Mile, Arizanović, Tanja, Raičević, Vera, "Relationships between water and sediment quality parameters and faecal bacteria content in the Palic-Ludas canal, Serbia" in International Journal of Environment and Pollution, 64, no. 4 (2018):292-309,
https://doi.org/10.1504/IJEP.2018.099463 . .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
AU  - Vujović, Bojana
AU  - Mirković, Milica
AU  - Rothballer, Michael
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4692
AB  - The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Listeria monocytogenes - Danger for health safety vegetable production
EP  - 31
SP  - 23
VL  - 120
DO  - 10.1016/j.micpath.2018.04.034
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera and Jovičić-Petrović, Jelena and Vujović, Bojana and Mirković, Milica and Rothballer, Michael",
year = "2018",
abstract = "The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 x 10(6) cells/mm(3) absolutely dry root) and spinach (1.39 x 10(6) cells/mm(3) absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the larval vessels of root. Our results suggest that L. monocytogenes is very good endophytic colonizer of vegetable plant roots.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Listeria monocytogenes - Danger for health safety vegetable production",
pages = "31-23",
volume = "120",
doi = "10.1016/j.micpath.2018.04.034"
}

Kljujev, I., Raičević, V., Jovičić-Petrović, J., Vujović, B., Mirković, M.,& Rothballer, M.. (2018). Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 120, 23-31.
https://doi.org/10.1016/j.micpath.2018.04.034

Kljujev I, Raičević V, Jovičić-Petrović J, Vujović B, Mirković M, Rothballer M. Listeria monocytogenes - Danger for health safety vegetable production. in Microbial Pathogenesis. 2018;120:23-31.
doi:10.1016/j.micpath.2018.04.034 .

Kljujev, Igor, Raičević, Vera, Jovičić-Petrović, Jelena, Vujović, Bojana, Mirković, Milica, Rothballer, Michael, "Listeria monocytogenes - Danger for health safety vegetable production" in Microbial Pathogenesis, 120 (2018):23-31,
https://doi.org/10.1016/j.micpath.2018.04.034 . .

TY  - JOUR
AU  - Kljujev, Igor
AU  - Raičević, Vera
AU  - Vujović, Bojana
AU  - Rothballer, Michael
AU  - Schmid, Michael
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4720
AB  - Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production
EP  - 207
SP  - 199
VL  - 115
DO  - 10.1016/j.micpath.2017.12.020
ER  - 

@article{
author = "Kljujev, Igor and Raičević, Vera and Vujović, Bojana and Rothballer, Michael and Schmid, Michael",
year = "2018",
abstract = "Contamination of vegetables and fruits is the result of presence of human pathogen bacteria which can contaminate products in any part of production chain. There is an evidence of presence of: Salmonella spp. on the fresh vegetables and Salmonellosis is connected with tomato, sprouts, cantaloupe etc. The goal of this research is transmission of pathogen bacteria from irrigation water to plants and studying/monitoring the ability of the Salmonella spp. to colonize the surface and interior (endophytic colonization) of root at different vegetable species. Transmission of three Salmonella spp. strains from irrigation water to plants, as well as colonization of plants by these bacteria was investigated by using Fluorescence In Situ Hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM). All tested Salmonella spp. strains showed ability to more or less colonize the surface and interior niches of the root, stem and leaf of the investigated plant species. These bacteria also were found in plant cells cytoplasm, although the mechanism of their entrance has not been clarified yet.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production",
pages = "207-199",
volume = "115",
doi = "10.1016/j.micpath.2017.12.020"
}

Kljujev, I., Raičević, V., Vujović, B., Rothballer, M.,& Schmid, M.. (2018). Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 115, 199-207.
https://doi.org/10.1016/j.micpath.2017.12.020

Kljujev I, Raičević V, Vujović B, Rothballer M, Schmid M. Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production. in Microbial Pathogenesis. 2018;115:199-207.
doi:10.1016/j.micpath.2017.12.020 .

Kljujev, Igor, Raičević, Vera, Vujović, Bojana, Rothballer, Michael, Schmid, Michael, "Salmonella as an endophytic colonizer of plants - A risk for health safety vegetable production" in Microbial Pathogenesis, 115 (2018):199-207,
https://doi.org/10.1016/j.micpath.2017.12.020 . .

TY  - JOUR
AU  - Vujović, Bojana
AU  - Rudić, Željka
AU  - Kljujev, Igor
AU  - Rajković, Dobrica
AU  - Božić, Mile
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4228
AB  - Biofilm formation is one of the main characteristic of Pseudomonas aeruginosa strains. This ability provide bacteria to survive in different, usually restrictivesss environmental conditions. In the same time, biofilm forming is a risk in a many field of industry, mainly in food industry. It is known that diverse P. aeruginosa strains from various environmental sources have different potention to form biofilms. In this paper it is examinated the potention to form biofilm four isolates of P. aeruginosa and based of this results, tested isolates have classificated into four groups. Biofilm formation tested in microtitar plates during the 24 hours at changed conditions of temperature (37°C and 22°C), pH (6 and 7) and concentration of NaCl (1% and 2%). Obtained results have shown that isolated P. aeruginosa have unsimilar ability to form biofilms. Biomass of formed biofilm was greater at 22°C than at 37°C. Also, acid conditions and higher concentration of salt had no inhibitory effect to biofilm forming process.
AB  - Stvaranje biofilmova predstavlja jednu od glavnih osobina Pseudomonas aeruginosa koja omogućava ovoj bakteriji preživljavanje u različitim, često nepovoljnim uslovima životne sredine. Međutim, formiranje biofilmova predstavlja i faktor rizika u mnogim oblastima industrije, posebno prehrambene, pošto prisustvo čvrstih površina pruža dobre uslove za formiranje biofilma. Kako je poznato da izolati P. aeruginosa koji potiču iz različitih uzoraka poreklom iz životne sredine imaju različit potencijal da formiraju biofilmove, u ovom radu je ispitana sposobnost formiranja biofilmova četiri izolata ove bakterije i izvršena njihova klasifikacija u odnosu na ovu osobinu. Primenjen je statički test u mikrotitar pločama u trajanju od 24 sata u različitim uslovima temperature (37°C i 22°C), pH (6 i 7) i koncentracije NaCl (1 i 2%). Dobijeni rezultati su pokazali da izolati P. aeruginosa imaju različitu sposobnost da formiraju biofilmove, da je masa formiranih biofilmova bila veća na 22°C, kao i da promenjeni uslovi sredine nisu inhibirali stvaranje biofilmova.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Potential of pseudomonas aeruginosa environmental isolates for biofilm formation
T1  - Potencijal formiranja biofilmova pseudomonas aeruginosa iz životne sredine
EP  - 454
IS  - 3
SP  - 449
VL  - 57
DO  - 10.5937/ZasMat1603449V
ER  - 

@article{
author = "Vujović, Bojana and Rudić, Željka and Kljujev, Igor and Rajković, Dobrica and Božić, Mile and Raičević, Vera",
year = "2016",
abstract = "Biofilm formation is one of the main characteristic of Pseudomonas aeruginosa strains. This ability provide bacteria to survive in different, usually restrictivesss environmental conditions. In the same time, biofilm forming is a risk in a many field of industry, mainly in food industry. It is known that diverse P. aeruginosa strains from various environmental sources have different potention to form biofilms. In this paper it is examinated the potention to form biofilm four isolates of P. aeruginosa and based of this results, tested isolates have classificated into four groups. Biofilm formation tested in microtitar plates during the 24 hours at changed conditions of temperature (37°C and 22°C), pH (6 and 7) and concentration of NaCl (1% and 2%). Obtained results have shown that isolated P. aeruginosa have unsimilar ability to form biofilms. Biomass of formed biofilm was greater at 22°C than at 37°C. Also, acid conditions and higher concentration of salt had no inhibitory effect to biofilm forming process., Stvaranje biofilmova predstavlja jednu od glavnih osobina Pseudomonas aeruginosa koja omogućava ovoj bakteriji preživljavanje u različitim, često nepovoljnim uslovima životne sredine. Međutim, formiranje biofilmova predstavlja i faktor rizika u mnogim oblastima industrije, posebno prehrambene, pošto prisustvo čvrstih površina pruža dobre uslove za formiranje biofilma. Kako je poznato da izolati P. aeruginosa koji potiču iz različitih uzoraka poreklom iz životne sredine imaju različit potencijal da formiraju biofilmove, u ovom radu je ispitana sposobnost formiranja biofilmova četiri izolata ove bakterije i izvršena njihova klasifikacija u odnosu na ovu osobinu. Primenjen je statički test u mikrotitar pločama u trajanju od 24 sata u različitim uslovima temperature (37°C i 22°C), pH (6 i 7) i koncentracije NaCl (1 i 2%). Dobijeni rezultati su pokazali da izolati P. aeruginosa imaju različitu sposobnost da formiraju biofilmove, da je masa formiranih biofilmova bila veća na 22°C, kao i da promenjeni uslovi sredine nisu inhibirali stvaranje biofilmova.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Potential of pseudomonas aeruginosa environmental isolates for biofilm formation, Potencijal formiranja biofilmova pseudomonas aeruginosa iz životne sredine",
pages = "454-449",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603449V"
}

Vujović, B., Rudić, Ž., Kljujev, I., Rajković, D., Božić, M.,& Raičević, V.. (2016). Potential of pseudomonas aeruginosa environmental isolates for biofilm formation. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 449-454.
https://doi.org/10.5937/ZasMat1603449V

Vujović B, Rudić Ž, Kljujev I, Rajković D, Božić M, Raičević V. Potential of pseudomonas aeruginosa environmental isolates for biofilm formation. in Zaštita materijala. 2016;57(3):449-454.
doi:10.5937/ZasMat1603449V .

Vujović, Bojana, Rudić, Željka, Kljujev, Igor, Rajković, Dobrica, Božić, Mile, Raičević, Vera, "Potential of pseudomonas aeruginosa environmental isolates for biofilm formation" in Zaštita materijala, 57, no. 3 (2016):449-454,
https://doi.org/10.5937/ZasMat1603449V . .

TY  - JOUR
AU  - Vujović, Bojana
AU  - Teodorović, Smilja
AU  - Rudić, Željka
AU  - Božić, Mile
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4052
AB  - Pseudomonas aeruginosa is a globally distributed environmental bacterium, which is also a significant opportunistic pathogen of humans, animals and plants. It is considered that wide distribution of this bacterium is connected with its most significant constitutive property to form biofilms, and that this multicellular mode of growth, predominant in nature, serves as a protective mechanism against unfavourable environmental conditions. The work presented here examines the phenotypic diversity of Pseudomonas aeruginosa environmental isolates with respect to biofilm production capacity under different environmental conditions (temperature, pH, NaCl), production of virulence factors, and motility. The purpose of this work is to present the production of two quorum sensing-regulated virulence factors (rhamnolipids and pyocyanin), explore different motility tests (swimming, swarming and twitching) and discover potential relationship between assessed phenotypic features. Obtained results delineate environmental conditions coinciding with biofilm production and suggest a high correlation between rhamnolipid production levels and biofilm formation. Rhamnolipids affect motility competence, yet only the flagellum-mediated swimming motility has significant impact on the biofilm formation potential. Although it is challenging to demarcate a definitive, clear correlation between parameters tested, rhamnolipid content appears to serve as a link between the tested phenotypic factors.
PB  - IWA Publishing, London
T2  - Water Science and Technology-Water Supply
T1  - Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia)
EP  - 1377
IS  - 5
SP  - 1370
VL  - 16
DO  - 10.2166/ws.2016.061
ER  - 

@article{
author = "Vujović, Bojana and Teodorović, Smilja and Rudić, Željka and Božić, Mile and Raičević, Vera",
year = "2016",
abstract = "Pseudomonas aeruginosa is a globally distributed environmental bacterium, which is also a significant opportunistic pathogen of humans, animals and plants. It is considered that wide distribution of this bacterium is connected with its most significant constitutive property to form biofilms, and that this multicellular mode of growth, predominant in nature, serves as a protective mechanism against unfavourable environmental conditions. The work presented here examines the phenotypic diversity of Pseudomonas aeruginosa environmental isolates with respect to biofilm production capacity under different environmental conditions (temperature, pH, NaCl), production of virulence factors, and motility. The purpose of this work is to present the production of two quorum sensing-regulated virulence factors (rhamnolipids and pyocyanin), explore different motility tests (swimming, swarming and twitching) and discover potential relationship between assessed phenotypic features. Obtained results delineate environmental conditions coinciding with biofilm production and suggest a high correlation between rhamnolipid production levels and biofilm formation. Rhamnolipids affect motility competence, yet only the flagellum-mediated swimming motility has significant impact on the biofilm formation potential. Although it is challenging to demarcate a definitive, clear correlation between parameters tested, rhamnolipid content appears to serve as a link between the tested phenotypic factors.",
publisher = "IWA Publishing, London",
journal = "Water Science and Technology-Water Supply",
title = "Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia)",
pages = "1377-1370",
number = "5",
volume = "16",
doi = "10.2166/ws.2016.061"
}

Vujović, B., Teodorović, S., Rudić, Ž., Božić, M.,& Raičević, V.. (2016). Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia). in Water Science and Technology-Water Supply
IWA Publishing, London., 16(5), 1370-1377.
https://doi.org/10.2166/ws.2016.061

Vujović B, Teodorović S, Rudić Ž, Božić M, Raičević V. Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia). in Water Science and Technology-Water Supply. 2016;16(5):1370-1377.
doi:10.2166/ws.2016.061 .

Vujović, Bojana, Teodorović, Smilja, Rudić, Željka, Božić, Mile, Raičević, Vera, "Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia)" in Water Science and Technology-Water Supply, 16, no. 5 (2016):1370-1377,
https://doi.org/10.2166/ws.2016.061 . .

TY  - CONF
AU  - Rudić, Zeljka
AU  - Vujović, Bojana
AU  - Bozić, Mile
AU  - Raičević, Vera
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5605
AB  - U ovom radu su prikazani rezultati bakteriološkog ispitivanja vode i sedimenta severnog i južnog dela jezera Ludaš. Jezero Ludaš je deo parka prirode Palić-Ludaš, a zbog velikog značaja u očuvanju ornitološkog diverziteta ovog područja od 1977. godine je zaštićeno i Ramsarskom konvencijom. Uzorci su sakupljani od novembra 2013. do marta 2015. godine i ukupno je analizirano 10 uzoraka vode i sedimenta. Kvalitet vode je ocenjen na osnovu ukupnog broja heterotrofnih bakterija (mezofila i psihrofila), fakultativnih oligotrofa, ukupnog broja koliformnih i fekalnih koliformnih bakterija, broja E.coli i fekalnih enterokoka. Ocenjivanje je izvršeno prema ekološkom i sanitarnom kriterijumu koristeći kriterijume Uredbe o kvalitetu površinskih voda i sedimenta Republike Srbije. Rezultati ukazuju na veliko variranje mikrobnog diverziteta tokom ispitivanog perioda. Na osnovu ukupnog broja bakterija, voda se može svrstati u II - IV klasu voda na severnoj strani, dok je na južnom delu voda u kategoriji III - V klase kvaliteta. Međutim, na osnovu indikatora fekalnog zagađenja, voda jezera na južnoj strani je značajno boljeg kvaliteta i u većini merenja odgovara kvalitetu I i II klase vodotoka. Razlike u sastavu saprofitne i potencijalno patogene grupe mikroorganizama zastupljene u različitim delovima jezera se mogu objasniti geografskim karakteristikama i vodi koja se kanalom Palić-Ludaš uliva u severni deo jezera, što utiče na broj bakterija indikatora fekalnog zagađenja. Veliko organsko opterećenje u južnom delu jezera se ogleda u značajno većem broju saprofitnih heterotrofa, a manjem autohtonih oligotrofa čime se smanjuje sposobnost autopurifikacije vode i ukazuje na ekološku ugroženost i ubrzan process eutrofikacije jezera Ludaš.
C3  - 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10
T1  - Lake Ludas Special Nature Reserve – Bacteriological Point Of View
T1  - Stanje specijalnog rezervata prirode “Ludaško jezero” sa bakteriološkog aspekta
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5605
ER  - 

@conference{
author = "Rudić, Zeljka and Vujović, Bojana and Bozić, Mile and Raičević, Vera",
year = "2015",
abstract = "U ovom radu su prikazani rezultati bakteriološkog ispitivanja vode i sedimenta severnog i južnog dela jezera Ludaš. Jezero Ludaš je deo parka prirode Palić-Ludaš, a zbog velikog značaja u očuvanju ornitološkog diverziteta ovog područja od 1977. godine je zaštićeno i Ramsarskom konvencijom. Uzorci su sakupljani od novembra 2013. do marta 2015. godine i ukupno je analizirano 10 uzoraka vode i sedimenta. Kvalitet vode je ocenjen na osnovu ukupnog broja heterotrofnih bakterija (mezofila i psihrofila), fakultativnih oligotrofa, ukupnog broja koliformnih i fekalnih koliformnih bakterija, broja E.coli i fekalnih enterokoka. Ocenjivanje je izvršeno prema ekološkom i sanitarnom kriterijumu koristeći kriterijume Uredbe o kvalitetu površinskih voda i sedimenta Republike Srbije. Rezultati ukazuju na veliko variranje mikrobnog diverziteta tokom ispitivanog perioda. Na osnovu ukupnog broja bakterija, voda se može svrstati u II - IV klasu voda na severnoj strani, dok je na južnom delu voda u kategoriji III - V klase kvaliteta. Međutim, na osnovu indikatora fekalnog zagađenja, voda jezera na južnoj strani je značajno boljeg kvaliteta i u većini merenja odgovara kvalitetu I i II klase vodotoka. Razlike u sastavu saprofitne i potencijalno patogene grupe mikroorganizama zastupljene u različitim delovima jezera se mogu objasniti geografskim karakteristikama i vodi koja se kanalom Palić-Ludaš uliva u severni deo jezera, što utiče na broj bakterija indikatora fekalnog zagađenja. Veliko organsko opterećenje u južnom delu jezera se ogleda u značajno većem broju saprofitnih heterotrofa, a manjem autohtonih oligotrofa čime se smanjuje sposobnost autopurifikacije vode i ukazuje na ekološku ugroženost i ubrzan process eutrofikacije jezera Ludaš.",
journal = "7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10",
title = "Lake Ludas Special Nature Reserve – Bacteriological Point Of View, Stanje specijalnog rezervata prirode “Ludaško jezero” sa bakteriološkog aspekta",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5605"
}

Rudić, Z., Vujović, B., Bozić, M.,& Raičević, V.. (2015). Lake Ludas Special Nature Reserve – Bacteriological Point Of View. in 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10.
https://hdl.handle.net/21.15107/rcub_agrospace_5605

Rudić Z, Vujović B, Bozić M, Raičević V. Lake Ludas Special Nature Reserve – Bacteriological Point Of View. in 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10. 2015;.
https://hdl.handle.net/21.15107/rcub_agrospace_5605 .

Rudić, Zeljka, Vujović, Bojana, Bozić, Mile, Raičević, Vera, "Lake Ludas Special Nature Reserve – Bacteriological Point Of View" in 7. International Conference “Water & Fish” Faculty of Agriculture, Belgrade-Zemun, Serbia, June, 10 (2015),
https://hdl.handle.net/21.15107/rcub_agrospace_5605 .