TY  - CONF
AU  - Obradović, B
AU  - Bugarski, Branko
AU  - Todosijević, Z
AU  - Nedović, Viktor
AU  - Bugarski, D
AU  - Vunjak-Novaković, G
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/874
AB  - Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.
PB  - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
C3  - Materials Science Forum
T1  - Mathematical modeling of cell distribution in alginate microbeads
EP  - 536
SP  - 531
VL  - 494
DO  - 10.4028/0-87849-971-7.531
ER  - 

@conference{
author = "Obradović, B and Bugarski, Branko and Todosijević, Z and Nedović, Viktor and Bugarski, D and Vunjak-Novaković, G",
year = "2005",
abstract = "Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.",
publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes",
journal = "Materials Science Forum",
title = "Mathematical modeling of cell distribution in alginate microbeads",
pages = "536-531",
volume = "494",
doi = "10.4028/0-87849-971-7.531"
}

Obradović, B., Bugarski, B., Todosijević, Z., Nedović, V., Bugarski, D.,& Vunjak-Novaković, G.. (2005). Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 531-536.
https://doi.org/10.4028/0-87849-971-7.531

Obradović B, Bugarski B, Todosijević Z, Nedović V, Bugarski D, Vunjak-Novaković G. Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum. 2005;494:531-536.
doi:10.4028/0-87849-971-7.531 .

Obradović, B, Bugarski, Branko, Todosijević, Z, Nedović, Viktor, Bugarski, D, Vunjak-Novaković, G, "Mathematical modeling of cell distribution in alginate microbeads" in Materials Science Forum, 494 (2005):531-536,
https://doi.org/10.4028/0-87849-971-7.531 . .

TY  - JOUR
AU  - Boltić, Z.
AU  - Petkovska, Menka
AU  - Obradović, B.
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2003
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/523
AB  - Results of this study have shown significantly prolonged release of selected antibiotic from liposome dispersion as compared to free antibiotic solution of the same initial concentration. Two models of non-steady one-dimensional diffusion were successfully applied to the experimental data and the antibiotic diffusion coefficients were estimated. In addition, the mass transfer resistance of the membrane was shown to be insignificant confirming the suitability of the applied experimental system. Since liposomes are known as systems with slower drug release, then, when it comes to their incorporation in the final form of a preparation for further experiments in vivo, the system of choice would be liposomes with an encapsulated antibiotic drug. Tfie established experimental system could be extended to other liposome formulations with respect to the release rate of the active components.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - In vitro evaluation of the controlled release of antibiotics from liposomes
T1  - In vitro ispitivanje kontrolisanog otpuštanja antibiotika iz liposoma
EP  - 595
IS  - 4
SP  - 589
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_523
ER  - 

@article{
author = "Boltić, Z. and Petkovska, Menka and Obradović, B. and Nedović, Viktor and Bugarski, Branko",
year = "2003",
abstract = "Results of this study have shown significantly prolonged release of selected antibiotic from liposome dispersion as compared to free antibiotic solution of the same initial concentration. Two models of non-steady one-dimensional diffusion were successfully applied to the experimental data and the antibiotic diffusion coefficients were estimated. In addition, the mass transfer resistance of the membrane was shown to be insignificant confirming the suitability of the applied experimental system. Since liposomes are known as systems with slower drug release, then, when it comes to their incorporation in the final form of a preparation for further experiments in vivo, the system of choice would be liposomes with an encapsulated antibiotic drug. Tfie established experimental system could be extended to other liposome formulations with respect to the release rate of the active components.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "In vitro evaluation of the controlled release of antibiotics from liposomes, In vitro ispitivanje kontrolisanog otpuštanja antibiotika iz liposoma",
pages = "595-589",
number = "4",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_523"
}

Boltić, Z., Petkovska, M., Obradović, B., Nedović, V.,& Bugarski, B.. (2003). In vitro evaluation of the controlled release of antibiotics from liposomes. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 9(4), 589-595.
https://hdl.handle.net/21.15107/rcub_agrospace_523

Boltić Z, Petkovska M, Obradović B, Nedović V, Bugarski B. In vitro evaluation of the controlled release of antibiotics from liposomes. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2003;9(4):589-595.
https://hdl.handle.net/21.15107/rcub_agrospace_523 .

Boltić, Z., Petkovska, Menka, Obradović, B., Nedović, Viktor, Bugarski, Branko, "In vitro evaluation of the controlled release of antibiotics from liposomes" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 9, no. 4 (2003):589-595,
https://hdl.handle.net/21.15107/rcub_agrospace_523 .

TY  - CONF
AU  - Nedović, Viktor
AU  - Obradović, B
AU  - Poncelet, Denis
AU  - Goosen, MFA
AU  - Leskošek-Čukalović, Ida
AU  - Bugarski, Branko
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/506
AB  - This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.
PB  - Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig
C3  - Practical Aspects of Encapsulation Technologies
T1  - Cell immobilisation by electrostatic droplet generation
EP  - 17
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_506
ER  - 

@conference{
author = "Nedović, Viktor and Obradović, B and Poncelet, Denis and Goosen, MFA and Leskošek-Čukalović, Ida and Bugarski, Branko",
year = "2002",
abstract = "This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.",
publisher = "Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig",
journal = "Practical Aspects of Encapsulation Technologies",
title = "Cell immobilisation by electrostatic droplet generation",
pages = "17-11",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_506"
}

Nedović, V., Obradović, B., Poncelet, D., Goosen, M., Leskošek-Čukalović, I.,& Bugarski, B.. (2002). Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies
Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig., 11-17.
https://hdl.handle.net/21.15107/rcub_agrospace_506

Nedović V, Obradović B, Poncelet D, Goosen M, Leskošek-Čukalović I, Bugarski B. Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies. 2002;:11-17.
https://hdl.handle.net/21.15107/rcub_agrospace_506 .

Nedović, Viktor, Obradović, B, Poncelet, Denis, Goosen, MFA, Leskošek-Čukalović, Ida, Bugarski, Branko, "Cell immobilisation by electrostatic droplet generation" in Practical Aspects of Encapsulation Technologies (2002):11-17,
https://hdl.handle.net/21.15107/rcub_agrospace_506 .

TY  - CONF
AU  - Nedović, Viktor
AU  - Obradović, B
AU  - Leskošek-Čukalović, Ida
AU  - Vunjak-Novaković, G
PY  - 2001
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/328
AB  - During recent years, immobilised yeast technology has gained increasing attention in the brewing industry. As a result of extensive research immobilised yeast technology is nowadays a well established technology for beer maturation and alcohol-free and low-alcohol beer production. However, in primary fermentation the situation is more complex and this process is still under scrutiny on the lab or pilot level. This paper describes several favourable immobilised cell - bioreactor systems for primary beer fermentation with particular emphasis on alginate-yeast microbeads - internal loop gas-lift bioreactor system, introduced into this field by our research group.
PB  - Springer, Dordrecht
C3  - Engineering and Manufacturing for Biotechnology, Vol 4
T1  - Immobilized yeast bioreactor systems for brewing - Recent achievements
EP  - 292
SP  - 277
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_328
ER  - 

@conference{
author = "Nedović, Viktor and Obradović, B and Leskošek-Čukalović, Ida and Vunjak-Novaković, G",
year = "2001",
abstract = "During recent years, immobilised yeast technology has gained increasing attention in the brewing industry. As a result of extensive research immobilised yeast technology is nowadays a well established technology for beer maturation and alcohol-free and low-alcohol beer production. However, in primary fermentation the situation is more complex and this process is still under scrutiny on the lab or pilot level. This paper describes several favourable immobilised cell - bioreactor systems for primary beer fermentation with particular emphasis on alginate-yeast microbeads - internal loop gas-lift bioreactor system, introduced into this field by our research group.",
publisher = "Springer, Dordrecht",
journal = "Engineering and Manufacturing for Biotechnology, Vol 4",
title = "Immobilized yeast bioreactor systems for brewing - Recent achievements",
pages = "292-277",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_328"
}

Nedović, V., Obradović, B., Leskošek-Čukalović, I.,& Vunjak-Novaković, G.. (2001). Immobilized yeast bioreactor systems for brewing - Recent achievements. in Engineering and Manufacturing for Biotechnology, Vol 4
Springer, Dordrecht., 4, 277-292.
https://hdl.handle.net/21.15107/rcub_agrospace_328

Nedović V, Obradović B, Leskošek-Čukalović I, Vunjak-Novaković G. Immobilized yeast bioreactor systems for brewing - Recent achievements. in Engineering and Manufacturing for Biotechnology, Vol 4. 2001;4:277-292.
https://hdl.handle.net/21.15107/rcub_agrospace_328 .

Nedović, Viktor, Obradović, B, Leskošek-Čukalović, Ida, Vunjak-Novaković, G, "Immobilized yeast bioreactor systems for brewing - Recent achievements" in Engineering and Manufacturing for Biotechnology, Vol 4, 4 (2001):277-292,
https://hdl.handle.net/21.15107/rcub_agrospace_328 .