TY  - JOUR
AU  - Petrović, Slobodan
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Aleksić, Nevenka
AU  - Maletić, Jelena
AU  - Ristanić, Marko
AU  - Stanimirović, Zoran
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5770
AB  - The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals.
PB  - Faculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria
T1  - The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions
EP  - 470
IS  - 4
SP  - 453
VL  - 70
DO  - 10.2478/acve-2020-0034
ER  - 

@article{
author = "Petrović, Slobodan and Maletić, Milan and Lakić, Nada and Aleksić, Nevenka and Maletić, Jelena and Ristanić, Marko and Stanimirović, Zoran",
year = "2021",
abstract = "The aim of the current research was to assess the effects of the feed additive made of lyophilised melon juice (source of superoxide dismutase, SOD) and inactivated live Saccharomyces cerevisiae (strain R397) cells added to the feed via the product containing high levels of organically bound selenium (source of selenium-dependant glutathione peroxidase, Se-GPx) on the semen quality of bulls in heat stress conditions. The 15 bulls chosen for the experiment were assigned to three equal groups (control-group C; treated group M, given the source of SOD; and group A, treated with the source of Se-GPx). The research was conducted in summer. The activities of SOD and Se-GPx in seminal plasma were determined spectrophotometrically. Computer-assisted semen analysis was done to determine the sperm counts, motility and velocity. The temperature and humidity were recorded with a digital data logger. The average SOD activity in the control bulls was significantly lower than in M (p<0.001) and A (p<0.001), whilst the average activities in the treated groups did not differ significantly (p=0.784). Higher average SOD activity compared to the control in the treated groups showed that both feed additives increased the antioxidative capacity of the seminal fluid. The average GPx activity in the control was significantly lower than in groups A (p=0.001) and M (p=0.005), whilst the two treatments did not lead to significantly different results (p=0.701). The analysis of relations between the activity of each enzyme and sperm motility and progressive motility in each of the bulls failed to detect a significant correlation. The analysis of the relation between THI (temperature-humidity index) and the activity of the antioxidative enzymes revealed that the increase in THI coincided with the decrease in the SOD activity in the control group, but with its increase in the treated groups (p>0.05). In all of the three groups with the increase in THI there was an increase in GPx activity (p>0.05). It can be concluded that in all of the three groups of bulls there was an increase in the activity of both enzymes in the seminal plasma, but the increase was significantly lower in the control. Thus, the antioxidative capacity of the seminal plasma of untreated bulls was proven to be lower in comparison with those of the treated animals.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria",
title = "The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions",
pages = "470-453",
number = "4",
volume = "70",
doi = "10.2478/acve-2020-0034"
}

Petrović, S., Maletić, M., Lakić, N., Aleksić, N., Maletić, J., Ristanić, M.,& Stanimirović, Z.. (2021). The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions. in Acta Veterinaria
Faculty of Veterinary Medicine, University of Belgrade., 70(4), 453-470.
https://doi.org/10.2478/acve-2020-0034

Petrović S, Maletić M, Lakić N, Aleksić N, Maletić J, Ristanić M, Stanimirović Z. The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions. in Acta Veterinaria. 2021;70(4):453-470.
doi:10.2478/acve-2020-0034 .

Petrović, Slobodan, Maletić, Milan, Lakić, Nada, Aleksić, Nevenka, Maletić, Jelena, Ristanić, Marko, Stanimirović, Zoran, "The Effects of Antioxidants Provided with Feed on Certain Quality Parameters of Bull Semen under Heat Stress Conditions" in Acta Veterinaria, 70, no. 4 (2021):453-470,
https://doi.org/10.2478/acve-2020-0034 . .

TY  - JOUR
AU  - Davidović, Vesna
AU  - Jovetić, Branko
AU  - Joksimović-Todorović, Mirjana
AU  - Stojanović, Bojan
AU  - Lazarević, Miodrag
AU  - Perišić, Predrag
AU  - Radivojević, Mihailo
AU  - Maletić, Milan
AU  - Miletić, Aleksandar
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4970
AB  - The aim of this study was to examine the effect of using tannin supplement in ration for mid-lactation dairy cows (90 +/- 17 days in milk) and the number of lactation on metabolic profile parameters values. Additionally, the effect of tannin supplementation on cows' production characteristics in the 2nd lactation was evaluated. Research was conducted on 80 Holstein cows divided into two groups of 40 cows (control and experimental group) that included 16 cows in 2nd and 12 in 3rd and 4th lactation. The tannin supplement Tanimil SCC (40 g/cow/day; 40% of tannins) was added in the total mixed ration (TMR) of experimental group, while the control group of cows was fed ration without the tannin supplement. The trial lasted 60 days. Dairy cows, fed diet supplemented with tannin, had markedly lower values (P lt 0.05) of B-hydroxybutyrate concentration (0.79 vs. 0.96 mmol/L) and lower blood urea concentration (5.89 vs. 6.93 mmol/L). Difference in the blood glucose concentrations (3.92 vs. 3.89 mmol/L) was not significant. The lactation number had no significant effect on values of metabolic profile parameters (concentration of glucose, B-hydroxybutyrate and urea). The effect of interaction of lactation number and tannin supplement on the values of examined parameters of metabolic profile was not significant. Supplementation of tannin in the diet for cows in the second lactation, had a positive effect (P lt 0.05) on the milk yield (4.42%) and yield of 4% fat corrected milk (FCM, 5.24%). The use of tannin, improved concentration (3.20 vs. 3.12%) and yield (from 1.16 to 1.24 kg/day) of milk protein (P lt 0.05). Addition of tannin also improved concentration and yield of milk fat, as well as the concentration of lactose. The supplementation of Tanimil SCC to mid-lactation dairy cows had a positive impacts on reduction of blood content of B-hydroxybutyrate and urea, and improved productive performances of cows in the second lactation.
PB  - Univ Ljubljana, Veterinary Faculty, Ljubljana
T2  - Slovenian Veterinary Research
T1  - The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics
EP  - 151
IS  - 4
SP  - 143
VL  - 56
DO  - 10.26873/SVR-552-2019
ER  - 

@article{
author = "Davidović, Vesna and Jovetić, Branko and Joksimović-Todorović, Mirjana and Stojanović, Bojan and Lazarević, Miodrag and Perišić, Predrag and Radivojević, Mihailo and Maletić, Milan and Miletić, Aleksandar",
year = "2019",
abstract = "The aim of this study was to examine the effect of using tannin supplement in ration for mid-lactation dairy cows (90 +/- 17 days in milk) and the number of lactation on metabolic profile parameters values. Additionally, the effect of tannin supplementation on cows' production characteristics in the 2nd lactation was evaluated. Research was conducted on 80 Holstein cows divided into two groups of 40 cows (control and experimental group) that included 16 cows in 2nd and 12 in 3rd and 4th lactation. The tannin supplement Tanimil SCC (40 g/cow/day; 40% of tannins) was added in the total mixed ration (TMR) of experimental group, while the control group of cows was fed ration without the tannin supplement. The trial lasted 60 days. Dairy cows, fed diet supplemented with tannin, had markedly lower values (P lt 0.05) of B-hydroxybutyrate concentration (0.79 vs. 0.96 mmol/L) and lower blood urea concentration (5.89 vs. 6.93 mmol/L). Difference in the blood glucose concentrations (3.92 vs. 3.89 mmol/L) was not significant. The lactation number had no significant effect on values of metabolic profile parameters (concentration of glucose, B-hydroxybutyrate and urea). The effect of interaction of lactation number and tannin supplement on the values of examined parameters of metabolic profile was not significant. Supplementation of tannin in the diet for cows in the second lactation, had a positive effect (P lt 0.05) on the milk yield (4.42%) and yield of 4% fat corrected milk (FCM, 5.24%). The use of tannin, improved concentration (3.20 vs. 3.12%) and yield (from 1.16 to 1.24 kg/day) of milk protein (P lt 0.05). Addition of tannin also improved concentration and yield of milk fat, as well as the concentration of lactose. The supplementation of Tanimil SCC to mid-lactation dairy cows had a positive impacts on reduction of blood content of B-hydroxybutyrate and urea, and improved productive performances of cows in the second lactation.",
publisher = "Univ Ljubljana, Veterinary Faculty, Ljubljana",
journal = "Slovenian Veterinary Research",
title = "The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics",
pages = "151-143",
number = "4",
volume = "56",
doi = "10.26873/SVR-552-2019"
}

Davidović, V., Jovetić, B., Joksimović-Todorović, M., Stojanović, B., Lazarević, M., Perišić, P., Radivojević, M., Maletić, M.,& Miletić, A.. (2019). The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics. in Slovenian Veterinary Research
Univ Ljubljana, Veterinary Faculty, Ljubljana., 56(4), 143-151.
https://doi.org/10.26873/SVR-552-2019

Davidović V, Jovetić B, Joksimović-Todorović M, Stojanović B, Lazarević M, Perišić P, Radivojević M, Maletić M, Miletić A. The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics. in Slovenian Veterinary Research. 2019;56(4):143-151.
doi:10.26873/SVR-552-2019 .

Davidović, Vesna, Jovetić, Branko, Joksimović-Todorović, Mirjana, Stojanović, Bojan, Lazarević, Miodrag, Perišić, Predrag, Radivojević, Mihailo, Maletić, Milan, Miletić, Aleksandar, "The effect of tannin supplementation of mid-lactation dairy cows diets on metabolic profile parameters and production characteristics" in Slovenian Veterinary Research, 56, no. 4 (2019):143-151,
https://doi.org/10.26873/SVR-552-2019 . .

TY  - JOUR
AU  - Maletić, Milan
AU  - Paprikić, Nevres
AU  - Lazarević, Miodrag
AU  - Hodzić, Aida
AU  - Davidović, Vesna
AU  - Stanisić, Ljubodrag
AU  - Stanimirović, Zoran
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5107
AB  - Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle
EP  - 163
IS  - 2
SP  - 153
VL  - 69
DO  - 10.2478/acve-2019-0012
ER  - 

@article{
author = "Maletić, Milan and Paprikić, Nevres and Lazarević, Miodrag and Hodzić, Aida and Davidović, Vesna and Stanisić, Ljubodrag and Stanimirović, Zoran",
year = "2019",
abstract = "Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p = 0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (-0.54 degrees C) had significantly higher (p = 0.004) average FPD values than those with AB genotype (-0.58 degrees C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle",
pages = "163-153",
number = "2",
volume = "69",
doi = "10.2478/acve-2019-0012"
}

Maletić, M., Paprikić, N., Lazarević, M., Hodzić, A., Davidović, V., Stanisić, L.,& Stanimirović, Z.. (2019). Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(2), 153-163.
https://doi.org/10.2478/acve-2019-0012

Maletić M, Paprikić N, Lazarević M, Hodzić A, Davidović V, Stanisić L, Stanimirović Z. Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle. in Acta Veterinaria-Beograd. 2019;69(2):153-163.
doi:10.2478/acve-2019-0012 .

Maletić, Milan, Paprikić, Nevres, Lazarević, Miodrag, Hodzić, Aida, Davidović, Vesna, Stanisić, Ljubodrag, Stanimirović, Zoran, "Insight in leptin gene polymorphism and impact on milk traits in autochtonous busha cattle" in Acta Veterinaria-Beograd, 69, no. 2 (2019):153-163,
https://doi.org/10.2478/acve-2019-0012 . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Zivkov-Balos, Milica
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Stanković, Branislav
AU  - Jajić, Igor
AU  - Maletić, Milan
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3762
AB  - Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.
PB  - Slovak Univ Agriculture Nitra, Nitra
T2  - Journal of Microbiology Biotechnology and Food Sciences
T1  - Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days
EP  - 3
SP  - 1
VL  - 4
DO  - 10.15414/jmbfs.2015.4.special2.1-3
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, Ivan and Jotanović, Stoja and Zivkov-Balos, Milica and Milovanović, Aleksandar and Barna, Tomislav and Stanković, Branislav and Jajić, Igor and Maletić, Milan",
year = "2015",
abstract = "Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.",
publisher = "Slovak Univ Agriculture Nitra, Nitra",
journal = "Journal of Microbiology Biotechnology and Food Sciences",
title = "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days",
pages = "3-1",
volume = "4",
doi = "10.15414/jmbfs.2015.4.special2.1-3"
}

Apić, J., Vakanjac, S., Radović, I., Jotanović, S., Zivkov-Balos, M., Milovanović, A., Barna, T., Stanković, B., Jajić, I.,& Maletić, M.. (2015). Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences
Slovak Univ Agriculture Nitra, Nitra., 4, 1-3.
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3

Apić J, Vakanjac S, Radović I, Jotanović S, Zivkov-Balos M, Milovanović A, Barna T, Stanković B, Jajić I, Maletić M. Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences. 2015;4:1-3.
doi:10.15414/jmbfs.2015.4.special2.1-3 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, Ivan, Jotanović, Stoja, Zivkov-Balos, Milica, Milovanović, Aleksandar, Barna, Tomislav, Stanković, Branislav, Jajić, Igor, Maletić, Milan, "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days" in Journal of Microbiology Biotechnology and Food Sciences, 4 (2015):1-3,
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3 . .