TY  - JOUR
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Menković, Jelena
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6326
AB  - During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.
PB  - APS Publications
T2  - Plant Disease
T1  - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
EP  - 833
IS  - 107
SP  - 826
DO  - 10.1094/PDIS-06-22-1425-RE
ER  - 

@article{
author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2023",
abstract = "During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.",
publisher = "APS Publications",
journal = "Plant Disease",
title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia",
pages = "833-826",
number = "107",
doi = "10.1094/PDIS-06-22-1425-RE"
}

Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease
APS Publications.(107), 826-833.
https://doi.org/10.1094/PDIS-06-22-1425-RE

Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease. 2023;(107):826-833.
doi:10.1094/PDIS-06-22-1425-RE .

Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in Plant Disease, no. 107 (2023):826-833,
https://doi.org/10.1094/PDIS-06-22-1425-RE . .

TY  - JOUR
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Weldon, William A.
AU  - Stockwell, Virginia O.
AU  - Pothier, Joël F.
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6461
AB  - Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.
T2  - Frontiers in Plant Science
T2  - Frontiers in Plant Science
T1  - Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6461
ER  - 

@article{
author = "Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Weldon, William A. and Stockwell, Virginia O. and Pothier, Joël F.",
year = "2023",
abstract = "Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.",
journal = "Frontiers in Plant Science, Frontiers in Plant Science",
title = "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6461"
}

Kałużna, M., Prokić, A., Obradović, A., Weldon, W. A., Stockwell, V. O.,& Pothier, J. F.. (2023). Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science, 14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461

Kałużna M, Prokić A, Obradović A, Weldon WA, Stockwell VO, Pothier JF. Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science. 2023;14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Weldon, William A., Stockwell, Virginia O., Pothier, Joël F., "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics" in Frontiers in Plant Science, 14 (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

TY  - JOUR
AU  - Pothier, Joël F
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Rezzonico, Fabio
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6069
AB  - Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate closed genome and plasmid sequences in order to better understand the biology of this pathogen.
PB  - NLM (Medline)
T2  - Phytopathology
T1  - Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut
EP  - 960
IS  - 4
SP  - 956
VL  - 112
DO  - 10.1094/PHYTO-08-21-0356-A
ER  - 

@article{
author = "Pothier, Joël F and Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Rezzonico, Fabio",
year = "2022",
abstract = "Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate closed genome and plasmid sequences in order to better understand the biology of this pathogen.",
publisher = "NLM (Medline)",
journal = "Phytopathology",
title = "Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut",
pages = "960-956",
number = "4",
volume = "112",
doi = "10.1094/PHYTO-08-21-0356-A"
}

Pothier, J. F., Kałużna, M., Prokić, A., Obradović, A.,& Rezzonico, F.. (2022). Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut. in Phytopathology
NLM (Medline)., 112(4), 956-960.
https://doi.org/10.1094/PHYTO-08-21-0356-A

Pothier JF, Kałużna M, Prokić A, Obradović A, Rezzonico F. Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut. in Phytopathology. 2022;112(4):956-960.
doi:10.1094/PHYTO-08-21-0356-A .

Pothier, Joël F, Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Rezzonico, Fabio, "Complete Genome and Plasmid Sequence Data of Three Strains of Xanthomonas arboricola pv. corylina, the Bacterium Responsible for Bacterial Blight of Hazelnut" in Phytopathology, 112, no. 4 (2022):956-960,
https://doi.org/10.1094/PHYTO-08-21-0356-A . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Živković, Svetlana
AU  - Obradović, Aleksa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6024
AB  - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.
PB  - MDPI
T2  - Agronomy
T1  - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
IS  - 2
SP  - 235
VL  - 12
DO  - 10.3390/agronomy12020235
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Živković, Svetlana and Obradović, Aleksa",
year = "2022",
abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.",
publisher = "MDPI",
journal = "Agronomy",
title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia",
number = "2",
pages = "235",
volume = "12",
doi = "10.3390/agronomy12020235"
}

Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Živković, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy
MDPI., 12(2), 235.
https://doi.org/10.3390/agronomy12020235

Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Živković S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12(2):235.
doi:10.3390/agronomy12020235 .

Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Živković, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12, no. 2 (2022):235,
https://doi.org/10.3390/agronomy12020235 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6013
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
SP  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Danijela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
pages = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, D.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12, 803789.
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić D, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12:803789.
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Danijela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12 (2022):803789,
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - RPRT
AU  - Popovic, T.
AU  - Menković, J.
AU  - Pantelić, M.
AU  - Obradović, A.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6089
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Xanthomonas euvesicatoria Causing Bacterial Leaf Spot of Pepper (Capsicum annuum) in Montenegro
IS  - 5
SP  - 1514
VL  - 106
DO  - 10.1094/PDIS-08-21-1655-PDN
ER  - 

@techreport{
author = "Popovic, T. and Menković, J. and Pantelić, M. and Obradović, A.",
year = "2022",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Xanthomonas euvesicatoria Causing Bacterial Leaf Spot of Pepper (Capsicum annuum) in Montenegro",
number = "5",
pages = "1514",
volume = "106",
doi = "10.1094/PDIS-08-21-1655-PDN"
}

Popovic, T., Menković, J., Pantelić, M.,& Obradović, A.. (2022). First Report of Xanthomonas euvesicatoria Causing Bacterial Leaf Spot of Pepper (Capsicum annuum) in Montenegro. in Plant Disease
American Phytopathological Society., 106(5), 1514.
https://doi.org/10.1094/PDIS-08-21-1655-PDN

Popovic T, Menković J, Pantelić M, Obradović A. First Report of Xanthomonas euvesicatoria Causing Bacterial Leaf Spot of Pepper (Capsicum annuum) in Montenegro. in Plant Disease. 2022;106(5):1514.
doi:10.1094/PDIS-08-21-1655-PDN .

Popovic, T., Menković, J., Pantelić, M., Obradović, A., "First Report of Xanthomonas euvesicatoria Causing Bacterial Leaf Spot of Pepper (Capsicum annuum) in Montenegro" in Plant Disease, 106, no. 5 (2022):1514,
https://doi.org/10.1094/PDIS-08-21-1655-PDN . .

TY  - RPRT
AU  - Prokić, A.
AU  - Marković, T.
AU  - Menković, J.
AU  - Ivanović, M.
AU  - Obradović, A.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6055
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Xanthomonas campestris pv. campestris Causing Marginal Leaf Necrosis of Arugula (Eruca vesicaria subsp. sativa) in Serbia
IS  - 3
SP  - 1056
VL  - 106
DO  - 10.1094/PDIS-04-21-0740-PDN
ER  - 

@techreport{
author = "Prokić, A. and Marković, T. and Menković, J. and Ivanović, M. and Obradović, A.",
year = "2022",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Xanthomonas campestris pv. campestris Causing Marginal Leaf Necrosis of Arugula (Eruca vesicaria subsp. sativa) in Serbia",
number = "3",
pages = "1056",
volume = "106",
doi = "10.1094/PDIS-04-21-0740-PDN"
}

Prokić, A., Marković, T., Menković, J., Ivanović, M.,& Obradović, A.. (2022). First Report of Xanthomonas campestris pv. campestris Causing Marginal Leaf Necrosis of Arugula (Eruca vesicaria subsp. sativa) in Serbia. in Plant Disease
American Phytopathological Society., 106(3), 1056.
https://doi.org/10.1094/PDIS-04-21-0740-PDN

Prokić A, Marković T, Menković J, Ivanović M, Obradović A. First Report of Xanthomonas campestris pv. campestris Causing Marginal Leaf Necrosis of Arugula (Eruca vesicaria subsp. sativa) in Serbia. in Plant Disease. 2022;106(3):1056.
doi:10.1094/PDIS-04-21-0740-PDN .

Prokić, A., Marković, T., Menković, J., Ivanović, M., Obradović, A., "First Report of Xanthomonas campestris pv. campestris Causing Marginal Leaf Necrosis of Arugula (Eruca vesicaria subsp. sativa) in Serbia" in Plant Disease, 106, no. 3 (2022):1056,
https://doi.org/10.1094/PDIS-04-21-0740-PDN . .

TY  - RPRT
AU  - Obradović, A.
AU  - Stepanović, J.
AU  - Krnjaja, V.
AU  - Bulajić, A.
AU  - Stanković, G.
AU  - Stevanović, M.
AU  - Stanković, S.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6041
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia
IS  - 2
SP  - 758
VL  - 106
DO  - 10.1094/PDIS-04-21-0715-PDN
ER  - 

@techreport{
author = "Obradović, A. and Stepanović, J. and Krnjaja, V. and Bulajić, A. and Stanković, G. and Stevanović, M. and Stanković, S.",
year = "2022",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia",
number = "2",
pages = "758",
volume = "106",
doi = "10.1094/PDIS-04-21-0715-PDN"
}

Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2022). First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease
American Phytopathological Society., 106(2), 758.
https://doi.org/10.1094/PDIS-04-21-0715-PDN

Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease. 2022;106(2):758.
doi:10.1094/PDIS-04-21-0715-PDN .

Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M., Stanković, S., "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia" in Plant Disease, 106, no. 2 (2022):758,
https://doi.org/10.1094/PDIS-04-21-0715-PDN . .

TY  - JOUR
AU  - Stefani, Emilio
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Altin, Irem
AU  - Nagy, Ildikó K.
AU  - Kovács, Tamás
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5851
AB  - Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.
PB  - MDPI
T2  - Microorganisms
T1  - Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future
IS  - 5
SP  - 1056
VL  - 9
DO  - 10.3390/microorganisms9051056
ER  - 

@article{
author = "Stefani, Emilio and Obradović, Aleksa and Gašić, Katarina and Altin, Irem and Nagy, Ildikó K. and Kovács, Tamás",
year = "2021",
abstract = "Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future",
number = "5",
pages = "1056",
volume = "9",
doi = "10.3390/microorganisms9051056"
}

Stefani, E., Obradović, A., Gašić, K., Altin, I., Nagy, I. K.,& Kovács, T.. (2021). Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms
MDPI., 9(5), 1056.
https://doi.org/10.3390/microorganisms9051056

Stefani E, Obradović A, Gašić K, Altin I, Nagy IK, Kovács T. Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms. 2021;9(5):1056.
doi:10.3390/microorganisms9051056 .

Stefani, Emilio, Obradović, Aleksa, Gašić, Katarina, Altin, Irem, Nagy, Ildikó K., Kovács, Tamás, "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future" in Microorganisms, 9, no. 5 (2021):1056,
https://doi.org/10.3390/microorganisms9051056 . .

TY  - JOUR
AU  - Morelli, Massimiliano
AU  - Bahar, Ofir
AU  - Papadopoulou, Kalliope K.
AU  - Hopkins, Donald L.
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5264
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Editorial: Role of Endophytes in Plant Health and Defense Against Pathogens
VL  - 11
DO  - 10.3389/fpls.2020.01312
ER  - 

@article{
author = "Morelli, Massimiliano and Bahar, Ofir and Papadopoulou, Kalliope K. and Hopkins, Donald L. and Obradović, Aleksa",
year = "2020",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Editorial: Role of Endophytes in Plant Health and Defense Against Pathogens",
volume = "11",
doi = "10.3389/fpls.2020.01312"
}

Morelli, M., Bahar, O., Papadopoulou, K. K., Hopkins, D. L.,& Obradović, A.. (2020). Editorial: Role of Endophytes in Plant Health and Defense Against Pathogens. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fpls.2020.01312

Morelli M, Bahar O, Papadopoulou KK, Hopkins DL, Obradović A. Editorial: Role of Endophytes in Plant Health and Defense Against Pathogens. in Frontiers in Plant Science. 2020;11.
doi:10.3389/fpls.2020.01312 .

Morelli, Massimiliano, Bahar, Ofir, Papadopoulou, Kalliope K., Hopkins, Donald L., Obradović, Aleksa, "Editorial: Role of Endophytes in Plant Health and Defense Against Pathogens" in Frontiers in Plant Science, 11 (2020),
https://doi.org/10.3389/fpls.2020.01312 . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Pavlović, Z.
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5379
AB  - Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia
EP  - 691
IS  - 3
SP  - 685
VL  - 157
DO  - 10.1007/s10658-020-02019-4
ER  - 

@article{
author = "Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Ivanović, Milan and Gašić, K. and Pavlović, Z. and Obradović, Aleksa",
year = "2020",
abstract = "Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia",
pages = "691-685",
number = "3",
volume = "157",
doi = "10.1007/s10658-020-02019-4"
}

Prokić, A., Zlatković, N., Kuzmanović, N., Ivanović, M., Gašić, K., Pavlović, Z.,& Obradović, A.. (2020). Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 157(3), 685-691.
https://doi.org/10.1007/s10658-020-02019-4

Prokić A, Zlatković N, Kuzmanović N, Ivanović M, Gašić K, Pavlović Z, Obradović A. Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology. 2020;157(3):685-691.
doi:10.1007/s10658-020-02019-4 .

Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Ivanović, Milan, Gašić, K., Pavlović, Z., Obradović, Aleksa, "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia" in European Journal of Plant Pathology, 157, no. 3 (2020):685-691,
https://doi.org/10.1007/s10658-020-02019-4 . .

TY  - JOUR
AU  - Popović, T.
AU  - Menković, Jelena
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5325
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro
EP  - 561
IS  - 2
SP  - 560
VL  - 104
DO  - 10.1094/PDIS-07-19-1422-PDN
ER  - 

@article{
author = "Popović, T. and Menković, Jelena and Prokić, Andjelka and Obradović, Aleksa",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro",
pages = "561-560",
number = "2",
volume = "104",
doi = "10.1094/PDIS-07-19-1422-PDN"
}

Popović, T., Menković, J., Prokić, A.,& Obradović, A.. (2020). First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(2), 560-561.
https://doi.org/10.1094/PDIS-07-19-1422-PDN

Popović T, Menković J, Prokić A, Obradović A. First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease. 2020;104(2):560-561.
doi:10.1094/PDIS-07-19-1422-PDN .

Popović, T., Menković, Jelena, Prokić, Andjelka, Obradović, Aleksa, "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro" in Plant Disease, 104, no. 2 (2020):560-561,
https://doi.org/10.1094/PDIS-07-19-1422-PDN . .

TY  - JOUR
AU  - Udovički, Božidar
AU  - Đekić, Ilija
AU  - Stanković, S.
AU  - Obradović, A.
AU  - Rajković, Andreja
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5085
AB  - The influence of climatic conditions on the levels of fumonisins in maize grown in Serbia was studied over eight years (2008 to 2015), investigating the possible relationship between the levels of fumonisins contamination in maize in relation to registered weather/climatic conditions. Presence of these mycotoxins in maize after harvest was evaluated based on climatic conditions within two periods: 10 days before and 10 days after 50% silking. Categories of fumonisins levels were transformed into classes. Chi-square test for association was used in analysing relationships between results of fumonisins levels and calendar years. Mann-Whitney U-test was used to compare differences between meteorological data of two subsets in years with high and low fumonisins level. There was a statistically significant association between the fumonisins levels and calendar years chi(2)=247.954; (P lt 0.05). This study identified low precipitation and low humidity combined with high solar radiation as a critical parameter combination for elevated levels of fumonisins. The statistically significant difference in relation to some of the examined parameters suggests that maize in Serbian agro-climatic conditions is more susceptible to fungal colonisation, and subsequent fumonisin production in the period of 10 days before 50% silking.
PB  - Wageningen Academic Publishers, Wageningen
T2  - World Mycotoxin Journal
T1  - Impact of climatic conditions on fumonisins in maize grown in Serbia
EP  - 190
IS  - 2
SP  - 183
VL  - 12
DO  - 10.3920/WMJ2018.2364
ER  - 

@article{
author = "Udovički, Božidar and Đekić, Ilija and Stanković, S. and Obradović, A. and Rajković, Andreja",
year = "2019",
abstract = "The influence of climatic conditions on the levels of fumonisins in maize grown in Serbia was studied over eight years (2008 to 2015), investigating the possible relationship between the levels of fumonisins contamination in maize in relation to registered weather/climatic conditions. Presence of these mycotoxins in maize after harvest was evaluated based on climatic conditions within two periods: 10 days before and 10 days after 50% silking. Categories of fumonisins levels were transformed into classes. Chi-square test for association was used in analysing relationships between results of fumonisins levels and calendar years. Mann-Whitney U-test was used to compare differences between meteorological data of two subsets in years with high and low fumonisins level. There was a statistically significant association between the fumonisins levels and calendar years chi(2)=247.954; (P lt 0.05). This study identified low precipitation and low humidity combined with high solar radiation as a critical parameter combination for elevated levels of fumonisins. The statistically significant difference in relation to some of the examined parameters suggests that maize in Serbian agro-climatic conditions is more susceptible to fungal colonisation, and subsequent fumonisin production in the period of 10 days before 50% silking.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "World Mycotoxin Journal",
title = "Impact of climatic conditions on fumonisins in maize grown in Serbia",
pages = "190-183",
number = "2",
volume = "12",
doi = "10.3920/WMJ2018.2364"
}

Udovički, B., Đekić, I., Stanković, S., Obradović, A.,& Rajković, A.. (2019). Impact of climatic conditions on fumonisins in maize grown in Serbia. in World Mycotoxin Journal
Wageningen Academic Publishers, Wageningen., 12(2), 183-190.
https://doi.org/10.3920/WMJ2018.2364

Udovički B, Đekić I, Stanković S, Obradović A, Rajković A. Impact of climatic conditions on fumonisins in maize grown in Serbia. in World Mycotoxin Journal. 2019;12(2):183-190.
doi:10.3920/WMJ2018.2364 .

Udovički, Božidar, Đekić, Ilija, Stanković, S., Obradović, A., Rajković, Andreja, "Impact of climatic conditions on fumonisins in maize grown in Serbia" in World Mycotoxin Journal, 12, no. 2 (2019):183-190,
https://doi.org/10.3920/WMJ2018.2364 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Andjelka
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4912
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia
EP  - 2667
IS  - 10
SP  - 2667
VL  - 103
DO  - 10.1094/PDIS-12-18-2213-PDN
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Andjelka and Gašić, K. and Kuzmanović, Nemanja and Ivanović, Milan and Obradović, Aleksa",
year = "2019",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia",
pages = "2667-2667",
number = "10",
volume = "103",
doi = "10.1094/PDIS-12-18-2213-PDN"
}

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(10), 2667-2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667-2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Andjelka, Gašić, K., Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667-2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Newberry, Eric A.
AU  - Ebrahim, Mohamed
AU  - Timilsina, Sujan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
AU  - Bull, Carolee T.
AU  - Goss, Erica M.
AU  - Huguet-Tapia, Jose C.
AU  - Paret, Mathews L.
AU  - Jones, Jeffrey B.
AU  - Potnis, Neha
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5040
AB  - Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash
VL  - 10
DO  - 10.3389/fmicb.2019.00270
ER  - 

@article{
author = "Newberry, Eric A. and Ebrahim, Mohamed and Timilsina, Sujan and Zlatković, Nevena and Obradović, Aleksa and Bull, Carolee T. and Goss, Erica M. and Huguet-Tapia, Jose C. and Paret, Mathews L. and Jones, Jeffrey B. and Potnis, Neha",
year = "2019",
abstract = "Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash",
volume = "10",
doi = "10.3389/fmicb.2019.00270"
}

Newberry, E. A., Ebrahim, M., Timilsina, S., Zlatković, N., Obradović, A., Bull, C. T., Goss, E. M., Huguet-Tapia, J. C., Paret, M. L., Jones, J. B.,& Potnis, N.. (2019). Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00270

Newberry EA, Ebrahim M, Timilsina S, Zlatković N, Obradović A, Bull CT, Goss EM, Huguet-Tapia JC, Paret ML, Jones JB, Potnis N. Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00270 .

Newberry, Eric A., Ebrahim, Mohamed, Timilsina, Sujan, Zlatković, Nevena, Obradović, Aleksa, Bull, Carolee T., Goss, Erica M., Huguet-Tapia, Jose C., Paret, Mathews L., Jones, Jeffrey B., Potnis, Neha, "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00270 . .

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5009
AB  - Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material
EP  - 1052
IS  - 3
SP  - 1039
VL  - 51
DO  - 10.2298/GENSR1903039I
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Gašić, Katarina and Prokić, Andjelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2019",
abstract = "Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material",
pages = "1052-1039",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903039I"
}

Ivanović, M., Kuzmanović, N., Gašić, K., Prokić, A., Zlatković, N.,& Obradović, A.. (2019). Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika
Društvo genetičara Srbije, Beograd., 51(3), 1039-1052.
https://doi.org/10.2298/GENSR1903039I

Ivanović M, Kuzmanović N, Gašić K, Prokić A, Zlatković N, Obradović A. Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika. 2019;51(3):1039-1052.
doi:10.2298/GENSR1903039I .

Ivanović, Milan, Kuzmanović, Nemanja, Gašić, Katarina, Prokić, Andjelka, Zlatković, Nevena, Obradović, Aleksa, "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material" in Genetika, 51, no. 3 (2019):1039-1052,
https://doi.org/10.2298/GENSR1903039I . .

TY  - JOUR
AU  - Popović, Tamara
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5174
AB  - Plant production in Montenegro is becoming more important considering limited availability of land, climatic conditions and economic opportunities. The production profitability can be compromised by various plant pathogenic microorganisms such as fungi, bacteria, phytoplasmas, and viruses. Bacterial and phytoplasma incited diseases are the most devastating considering their spread and difficulty to control. In Montenegro, bacterial diseases of plants have not been sufficiently studied. However, several economically important bacteria were reported in the past. Erwinia amylovora, the causal agent of fire blight of pome fruits is widespread in the continental part of the country, causing significant damage and limiting production of pome fruits and susceptible ornamental plants. Pseudomonas syringae pv. syringae, the causal agent of citrus blast of mandarin, almost every year, depending on climatic conditions, causes the production losses along Montenegrian coast. Pseudomonas syringae, commonly known as a pathogen of stone fruits, was just studied as a pathogen of sour cherries. The presence of Xanthomonas arboricola pv. pruni on almond trees indicate the need to determine its possible presence in other host plants (peach, nectarine, plum, apricot). Agrobacterium tumefaciens was reported as causal agent of crown gall disease of grapevine. Considering vegetable bacterial diseases, the presence of Xanthomonas campestris pv. campestris, the causal agent of black rot and vascular bacterial disease, was confirmed on crucifers. On ornamental plants, Pseudomonas savastanoi pv. nerii was confirmed on oleander, while calla soft rot was caused by Pectobacterium carotovorum subsp. carotovorum.
AB  - Biljna proizvodnja u Crnoj Gori dobija sve veći značaj s obzirom na zemljišne, klimatske i ekonomske prilike. Rentabilnost biljne proizvodnje mogu ugroziti različiti patogeni mikroorganizmi kao što su: gljive, bakterije, fitoplazme i virusi. Po značaju, rasprostranjenosti, ali i nemogućnosti uspješne kontrole, izdvajaju se oboljenja bakteriozne i fitoplazmozne prirode. U Crnoj Gori bakterioze biljaka još uvijek nisu dovoljno proučene. Među saopštenjima o pojavi biljnih oboljenja značajnijeg intenziteta prikazano je i nekoliko ekonomski veoma značajnih bakterija. Erwinia amylovora, prouzrokovao bakteriozne plamenjače jabučastih voćaka, široko je rasprostranjena u kontinentalnom dijelu Crne Gore, gde prouzrokuje značajne štete i predstavlja ozbiljnu prijetnju uspešnom gajenju jabučastih voćaka i osjetljivih ukrasnih biljka. Pseudomonas syringae pv. syringae, prouzrokovao bakteriozne paleži lišća mandarine, gotovo svake godine, u zavisnosti od klimatskih prilika, prouzrokuje štete na Crnogorskom primorju. Pseudomonas syringae, kao opštepoznat patogen koštičavih voćaka, proučen je samo kao patogen višnje. Utvrđeno prisustvo patogena Xanthomonas arboricola pv. pruni na bademu, nameće potrebu da se utvrdi eventualno prisustvo patogena i u drugim lokalitetima kao i na drugim domaćinima (breskva, nektarina, šljiva, kajsija). Agrobacterium tumefaciens prouzrokuje bakteriozni rak vinove loze. Od povrtarskih biljaka, na kupusnjačama je utvrđeno prisustvo Xanthomonas campestris pv. campestris prouzrokovača crne truleži i sudovne bakterioze kupusnjača. Na ukrasnom bilju, kao patogen oleandera utvrđen je Pseudomonas savastanoi pv. nerii prouzrokovao raka oleandera, dok bakterioznu vlažnu trulež kale prouzrokuje Pectobacterium carotovorum subsp. carotovorum.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Overview of plant bacterial diseases research in Montenegro
T1  - Pregled proučavanja bakterioza biljaka u Crnoj Gori
EP  - 268
IS  - 4
SP  - 256
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5174
ER  - 

@article{
author = "Popović, Tamara and Obradović, Aleksa",
year = "2019",
abstract = "Plant production in Montenegro is becoming more important considering limited availability of land, climatic conditions and economic opportunities. The production profitability can be compromised by various plant pathogenic microorganisms such as fungi, bacteria, phytoplasmas, and viruses. Bacterial and phytoplasma incited diseases are the most devastating considering their spread and difficulty to control. In Montenegro, bacterial diseases of plants have not been sufficiently studied. However, several economically important bacteria were reported in the past. Erwinia amylovora, the causal agent of fire blight of pome fruits is widespread in the continental part of the country, causing significant damage and limiting production of pome fruits and susceptible ornamental plants. Pseudomonas syringae pv. syringae, the causal agent of citrus blast of mandarin, almost every year, depending on climatic conditions, causes the production losses along Montenegrian coast. Pseudomonas syringae, commonly known as a pathogen of stone fruits, was just studied as a pathogen of sour cherries. The presence of Xanthomonas arboricola pv. pruni on almond trees indicate the need to determine its possible presence in other host plants (peach, nectarine, plum, apricot). Agrobacterium tumefaciens was reported as causal agent of crown gall disease of grapevine. Considering vegetable bacterial diseases, the presence of Xanthomonas campestris pv. campestris, the causal agent of black rot and vascular bacterial disease, was confirmed on crucifers. On ornamental plants, Pseudomonas savastanoi pv. nerii was confirmed on oleander, while calla soft rot was caused by Pectobacterium carotovorum subsp. carotovorum., Biljna proizvodnja u Crnoj Gori dobija sve veći značaj s obzirom na zemljišne, klimatske i ekonomske prilike. Rentabilnost biljne proizvodnje mogu ugroziti različiti patogeni mikroorganizmi kao što su: gljive, bakterije, fitoplazme i virusi. Po značaju, rasprostranjenosti, ali i nemogućnosti uspješne kontrole, izdvajaju se oboljenja bakteriozne i fitoplazmozne prirode. U Crnoj Gori bakterioze biljaka još uvijek nisu dovoljno proučene. Među saopštenjima o pojavi biljnih oboljenja značajnijeg intenziteta prikazano je i nekoliko ekonomski veoma značajnih bakterija. Erwinia amylovora, prouzrokovao bakteriozne plamenjače jabučastih voćaka, široko je rasprostranjena u kontinentalnom dijelu Crne Gore, gde prouzrokuje značajne štete i predstavlja ozbiljnu prijetnju uspešnom gajenju jabučastih voćaka i osjetljivih ukrasnih biljka. Pseudomonas syringae pv. syringae, prouzrokovao bakteriozne paleži lišća mandarine, gotovo svake godine, u zavisnosti od klimatskih prilika, prouzrokuje štete na Crnogorskom primorju. Pseudomonas syringae, kao opštepoznat patogen koštičavih voćaka, proučen je samo kao patogen višnje. Utvrđeno prisustvo patogena Xanthomonas arboricola pv. pruni na bademu, nameće potrebu da se utvrdi eventualno prisustvo patogena i u drugim lokalitetima kao i na drugim domaćinima (breskva, nektarina, šljiva, kajsija). Agrobacterium tumefaciens prouzrokuje bakteriozni rak vinove loze. Od povrtarskih biljaka, na kupusnjačama je utvrđeno prisustvo Xanthomonas campestris pv. campestris prouzrokovača crne truleži i sudovne bakterioze kupusnjača. Na ukrasnom bilju, kao patogen oleandera utvrđen je Pseudomonas savastanoi pv. nerii prouzrokovao raka oleandera, dok bakterioznu vlažnu trulež kale prouzrokuje Pectobacterium carotovorum subsp. carotovorum.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Overview of plant bacterial diseases research in Montenegro, Pregled proučavanja bakterioza biljaka u Crnoj Gori",
pages = "268-256",
number = "4",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5174"
}

Popović, T.,& Obradović, A.. (2019). Overview of plant bacterial diseases research in Montenegro. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 47(4), 256-268.
https://hdl.handle.net/21.15107/rcub_agrospace_5174

Popović T, Obradović A. Overview of plant bacterial diseases research in Montenegro. in Biljni lekar. 2019;47(4):256-268.
https://hdl.handle.net/21.15107/rcub_agrospace_5174 .

Popović, Tamara, Obradović, Aleksa, "Overview of plant bacterial diseases research in Montenegro" in Biljni lekar, 47, no. 4 (2019):256-268,
https://hdl.handle.net/21.15107/rcub_agrospace_5174 .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5221
AB  - Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species.
AB  - Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of cucurbits
T1  - Bakterioze tikava
EP  - 445
IS  - 6
SP  - 431
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5221
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species., Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of cucurbits, Bakterioze tikava",
pages = "445-431",
number = "6",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5221"
}

Zlatković, N., Gašić, K., Prokić, A.,& Obradović, A.. (2019). Bacterial diseases of cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 47(6), 431-445.
https://hdl.handle.net/21.15107/rcub_agrospace_5221

Zlatković N, Gašić K, Prokić A, Obradović A. Bacterial diseases of cucurbits. in Biljni lekar. 2019;47(6):431-445.
https://hdl.handle.net/21.15107/rcub_agrospace_5221 .

Zlatković, Nevena, Gašić, Katarina, Prokić, Andjelka, Obradović, Aleksa, "Bacterial diseases of cucurbits" in Biljni lekar, 47, no. 6 (2019):431-445,
https://hdl.handle.net/21.15107/rcub_agrospace_5221 .

TY  - JOUR
AU  - Newberry, Eric A.
AU  - Ebrahim, Mohamed
AU  - Timilsina, Sujan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
AU  - Bull, Carolee T.
AU  - Goss, Erica M.
AU  - Huguet-Tapia, Jose C.
AU  - Paret, Mathews L.
AU  - Jones, Jeffrey B.
AU  - Potnis, Neha
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5045
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019)
VL  - 10
DO  - 10.3389/fmicb.2019.00963
ER  - 

@article{
author = "Newberry, Eric A. and Ebrahim, Mohamed and Timilsina, Sujan and Zlatković, Nevena and Obradović, Aleksa and Bull, Carolee T. and Goss, Erica M. and Huguet-Tapia, Jose C. and Paret, Mathews L. and Jones, Jeffrey B. and Potnis, Neha",
year = "2019",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019)",
volume = "10",
doi = "10.3389/fmicb.2019.00963"
}

Newberry, E. A., Ebrahim, M., Timilsina, S., Zlatković, N., Obradović, A., Bull, C. T., Goss, E. M., Huguet-Tapia, J. C., Paret, M. L., Jones, J. B.,& Potnis, N.. (2019). Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019). in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00963

Newberry EA, Ebrahim M, Timilsina S, Zlatković N, Obradović A, Bull CT, Goss EM, Huguet-Tapia JC, Paret ML, Jones JB, Potnis N. Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019). in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00963 .

Newberry, Eric A., Ebrahim, Mohamed, Timilsina, Sujan, Zlatković, Nevena, Obradović, Aleksa, Bull, Carolee T., Goss, Erica M., Huguet-Tapia, Jose C., Paret, Mathews L., Jones, Jeffrey B., Potnis, Neha, "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash (vol 10, 270, 2019)" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00963 . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5017
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain K Phi 1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96-98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier Sci Ltd, Oxford., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Andjelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .

TY  - CONF
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4646
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot
EP  - 133
IS  - 10
SP  - 132
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4646
ER  - 

@conference{
author = "Gašić, K. and Kuzmanović, Nemanja and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot",
pages = "133-132",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4646"
}

Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N.,& Obradović, A.. (2018). Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 132-133.
https://hdl.handle.net/21.15107/rcub_agrospace_4646

Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Obradović A. Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology. 2018;108(10):132-133.
https://hdl.handle.net/21.15107/rcub_agrospace_4646 .

Gašić, K., Kuzmanović, Nemanja, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Obradović, Aleksa, "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot" in Phytopathology, 108, no. 10 (2018):132-133,
https://hdl.handle.net/21.15107/rcub_agrospace_4646 .

TY  - CONF
AU  - Prokić, Andjelka
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4645
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro
EP  - 24
IS  - 10
SP  - 24
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4645
ER  - 

@conference{
author = "Prokić, Andjelka and Ivanović, Milan and Gašić, K. and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro",
pages = "24-24",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4645"
}

Prokić, A., Ivanović, M., Gašić, K., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2018). Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 24-24.
https://hdl.handle.net/21.15107/rcub_agrospace_4645

Prokić A, Ivanović M, Gašić K, Kuzmanović N, Zlatković N, Obradović A. Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology. 2018;108(10):24-24.
https://hdl.handle.net/21.15107/rcub_agrospace_4645 .

Prokić, Andjelka, Ivanović, Milan, Gašić, K., Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro" in Phytopathology, 108, no. 10 (2018):24-24,
https://hdl.handle.net/21.15107/rcub_agrospace_4645 .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4839
AB  - Crown gall is considered one of the most important and widespread bacterial diseases of grapevine (Vitis vinifera L.). Disease is present in almost all grapevine-growing areas worldwide. Crown gall is an economically important plant disease of grapevine, especially in nurseries and newly established vineyards. Typical symptoms of grapevine crown gall disease include tumor formation on the aerial parts of host plants. Agrobacterium vitis is primary species causing this disease. A. vitis colonizes grapevine systemically and can persists latently in symptomless plant material. It is also detected as an epiphyte on grapevine and can survive in soil exclusively in association with host plants. Crown gall can be efficiently disseminated to distant geographical areas via international trade of propagation material, which is considered the main pathway of disease introduction and spread. In Serbia, crown gall was observed sporadically on grapevine, although it occurred with high incidence and severity in some years. Because there are no effective curative control measures, the disease is especially challenging to manage. Therefore, the disease management strategy is based on preventive measures. Use of pathogen-free planting material in areas with no history of the crown gall represents main prerequisite for the disease prevention. Biological control and production of resistant grape varieties are promising as future control measures.
AB  - Bakteriozni rak smatra se jednim od najvažnijih i najrasprostranjenijih bakte-rioznih oboljenja vinove loze (Vitis vinifera L.). Ustanovljen je u gotovo svim zemljama gde se gaji vinova loza. Bakteriozni rak je ekonomski vrlo značajno oboljenje vinove loze, a posebno je ozbiljno u rasadnicima i mladim vinogradima. Tipični simptomi bakterioznog raka ispoljavaju se u vidu tumora na nadzemnim delovima vinove loze. Vrsta Agrobacterium vitis glavni je prouzrokovač ovog obo-ljenja. Patogen sistemično zaražava vinovu lozu i prisutan je u sprovodnom tkivu domaćina, a može biti latentno prisutan u asimptomatičnom biljnom materijalu. Takođe, patogen je detektovan i kao epifit na površini različitih organa vinove loze, kao i u rizosferi i zemljištu u blizini biljaka domaćina. A. vitis može biti raširen na velike udaljenosti putem naizgled zdravog sadnog materijala, što predstavlja najznačajniji način introdukcije i širenja bolesti. U Srbiji, bakteriozni rak se javlja sporadično, mada je u pojedinim godinama zabeležena jača pojava ovog obolje-nja uz visoku stopu zaraženih biljaka i izražene štete. Kontrola bakterioznog raka nije laka, s obzirom da ne postoje efikasne kurativne mere. Stoga se strategija zaštite prvenstveno zasniva na preventivnim merama. Korišćenje zdravog sad-nog materijala i zasnivanje vinograda na površinama koje nisu kontaminirane patogenom neizostavna je mera. Biološka kontrola i proizvodnja otpornih sorti predstavljaju obećavajuće mere kontrole u budućnosti.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Crown gall of grapevine
T1  - Bakteriozni rak vinove loze
EP  - 673
IS  - 6
SP  - 659
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4839
ER  - 

@article{
author = "Kuzmanović, Nemanja and Obradović, Aleksa",
year = "2018",
abstract = "Crown gall is considered one of the most important and widespread bacterial diseases of grapevine (Vitis vinifera L.). Disease is present in almost all grapevine-growing areas worldwide. Crown gall is an economically important plant disease of grapevine, especially in nurseries and newly established vineyards. Typical symptoms of grapevine crown gall disease include tumor formation on the aerial parts of host plants. Agrobacterium vitis is primary species causing this disease. A. vitis colonizes grapevine systemically and can persists latently in symptomless plant material. It is also detected as an epiphyte on grapevine and can survive in soil exclusively in association with host plants. Crown gall can be efficiently disseminated to distant geographical areas via international trade of propagation material, which is considered the main pathway of disease introduction and spread. In Serbia, crown gall was observed sporadically on grapevine, although it occurred with high incidence and severity in some years. Because there are no effective curative control measures, the disease is especially challenging to manage. Therefore, the disease management strategy is based on preventive measures. Use of pathogen-free planting material in areas with no history of the crown gall represents main prerequisite for the disease prevention. Biological control and production of resistant grape varieties are promising as future control measures., Bakteriozni rak smatra se jednim od najvažnijih i najrasprostranjenijih bakte-rioznih oboljenja vinove loze (Vitis vinifera L.). Ustanovljen je u gotovo svim zemljama gde se gaji vinova loza. Bakteriozni rak je ekonomski vrlo značajno oboljenje vinove loze, a posebno je ozbiljno u rasadnicima i mladim vinogradima. Tipični simptomi bakterioznog raka ispoljavaju se u vidu tumora na nadzemnim delovima vinove loze. Vrsta Agrobacterium vitis glavni je prouzrokovač ovog obo-ljenja. Patogen sistemično zaražava vinovu lozu i prisutan je u sprovodnom tkivu domaćina, a može biti latentno prisutan u asimptomatičnom biljnom materijalu. Takođe, patogen je detektovan i kao epifit na površini različitih organa vinove loze, kao i u rizosferi i zemljištu u blizini biljaka domaćina. A. vitis može biti raširen na velike udaljenosti putem naizgled zdravog sadnog materijala, što predstavlja najznačajniji način introdukcije i širenja bolesti. U Srbiji, bakteriozni rak se javlja sporadično, mada je u pojedinim godinama zabeležena jača pojava ovog obolje-nja uz visoku stopu zaraženih biljaka i izražene štete. Kontrola bakterioznog raka nije laka, s obzirom da ne postoje efikasne kurativne mere. Stoga se strategija zaštite prvenstveno zasniva na preventivnim merama. Korišćenje zdravog sad-nog materijala i zasnivanje vinograda na površinama koje nisu kontaminirane patogenom neizostavna je mera. Biološka kontrola i proizvodnja otpornih sorti predstavljaju obećavajuće mere kontrole u budućnosti.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Crown gall of grapevine, Bakteriozni rak vinove loze",
pages = "673-659",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4839"
}

Kuzmanović, N.,& Obradović, A.. (2018). Crown gall of grapevine. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 659-673.
https://hdl.handle.net/21.15107/rcub_agrospace_4839

Kuzmanović N, Obradović A. Crown gall of grapevine. in Biljni lekar. 2018;46(6):659-673.
https://hdl.handle.net/21.15107/rcub_agrospace_4839 .

Kuzmanović, Nemanja, Obradović, Aleksa, "Crown gall of grapevine" in Biljni lekar, 46, no. 6 (2018):659-673,
https://hdl.handle.net/21.15107/rcub_agrospace_4839 .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4662
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 

@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Andjelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}

Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02021

Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .

Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Andjelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Dreo, Tanja
AU  - Obradović, Aleksa
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4838
AB  - Bacterial blight of grapevine, caused by phytopathogenic bacterium Xylophilus ampelinus, is economically important disease that can significantly affect grapevine production, leading to the overall yield reduction and reduced vitality of infected grapevines. In regions where disease was recorded, losses in production, especially in susceptible varieties, can reach up to 80% of total yield. The bacterium infects only grapevine. In infected plants, the pathogen is located in the vascular tissues (xylem) from where it spreads further, causing a systemic infection of the host plant. During vegetation it is transmitted from plant to plant to short distance and the main source of inoculum for long distance dissemination are infected cuttings used either as rootstocks or grafting material. There are no completely resistant varieties or effective methods for controlling the disease. In Serbia X. ampelinus has a quarantine status. Therefore, it is of utmost importance to prevent introduction of the pathogen by inspecting the health of reproductive plant material, especially of those originating from countries where the pathogen is already present.
AB  - Bakteriozna plamenjača vinove loze, koju prouzrokuje fitopatogena bakterija Xylophilus ampelinus, je ekonomski značajno oboljenje koje može ugroziti proizvodnju vinove loze umanjujući ukupan prinos i smanjujući dugovečnost zaraženih biljaka. U regionima gde je zabeleženo prisustvo bolesti gubici u proizvodnji, naročito kod osetljivih sorti, mogu dostići i do 80% ukupnog prinosa. Krug domaćina ove bakterije ograničen je samo na vinovu lozu. U zaraženim biljkama patogen se nalazi u sudovnom sistemu (ksilemu) odakle se dalje širi prouzrokujući sistemičnu infekciju biljke domaćina. Tokom vegetacije prenosi se u neposrednoj okolini izvora zaraze sa biljke na biljku, a na veću udaljenost zaraženim sadnim materijalom koji predstavlja primarni izvor inokuluma. Ne postoje potpuno otporne sorte ni dovoljno efikasne metode zaštite od ove bolesti. U Srbiji X. ampelinus ima karantinski status. Stoga je od najvećeg značaja sprečavanje unošenja patogena kontrolom zdravstvene ispravnosti biljnog materijala za reprodukciju, posebno iz zemalja gde je utvrđeno prisustvo patogena.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial blight of grapevine: Xylophilus ampelinus
T1  - Bakteriozna plamenjača vinove loze - Xylophilus ampelinus
EP  - 680
IS  - 6
SP  - 674
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4838
ER  - 

@article{
author = "Prokić, Andjelka and Dreo, Tanja and Obradović, Aleksa",
year = "2018",
abstract = "Bacterial blight of grapevine, caused by phytopathogenic bacterium Xylophilus ampelinus, is economically important disease that can significantly affect grapevine production, leading to the overall yield reduction and reduced vitality of infected grapevines. In regions where disease was recorded, losses in production, especially in susceptible varieties, can reach up to 80% of total yield. The bacterium infects only grapevine. In infected plants, the pathogen is located in the vascular tissues (xylem) from where it spreads further, causing a systemic infection of the host plant. During vegetation it is transmitted from plant to plant to short distance and the main source of inoculum for long distance dissemination are infected cuttings used either as rootstocks or grafting material. There are no completely resistant varieties or effective methods for controlling the disease. In Serbia X. ampelinus has a quarantine status. Therefore, it is of utmost importance to prevent introduction of the pathogen by inspecting the health of reproductive plant material, especially of those originating from countries where the pathogen is already present., Bakteriozna plamenjača vinove loze, koju prouzrokuje fitopatogena bakterija Xylophilus ampelinus, je ekonomski značajno oboljenje koje može ugroziti proizvodnju vinove loze umanjujući ukupan prinos i smanjujući dugovečnost zaraženih biljaka. U regionima gde je zabeleženo prisustvo bolesti gubici u proizvodnji, naročito kod osetljivih sorti, mogu dostići i do 80% ukupnog prinosa. Krug domaćina ove bakterije ograničen je samo na vinovu lozu. U zaraženim biljkama patogen se nalazi u sudovnom sistemu (ksilemu) odakle se dalje širi prouzrokujući sistemičnu infekciju biljke domaćina. Tokom vegetacije prenosi se u neposrednoj okolini izvora zaraze sa biljke na biljku, a na veću udaljenost zaraženim sadnim materijalom koji predstavlja primarni izvor inokuluma. Ne postoje potpuno otporne sorte ni dovoljno efikasne metode zaštite od ove bolesti. U Srbiji X. ampelinus ima karantinski status. Stoga je od najvećeg značaja sprečavanje unošenja patogena kontrolom zdravstvene ispravnosti biljnog materijala za reprodukciju, posebno iz zemalja gde je utvrđeno prisustvo patogena.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial blight of grapevine: Xylophilus ampelinus, Bakteriozna plamenjača vinove loze - Xylophilus ampelinus",
pages = "680-674",
number = "6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4838"
}

Prokić, A., Dreo, T.,& Obradović, A.. (2018). Bacterial blight of grapevine: Xylophilus ampelinus. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 46(6), 674-680.
https://hdl.handle.net/21.15107/rcub_agrospace_4838

Prokić A, Dreo T, Obradović A. Bacterial blight of grapevine: Xylophilus ampelinus. in Biljni lekar. 2018;46(6):674-680.
https://hdl.handle.net/21.15107/rcub_agrospace_4838 .

Prokić, Andjelka, Dreo, Tanja, Obradović, Aleksa, "Bacterial blight of grapevine: Xylophilus ampelinus" in Biljni lekar, 46, no. 6 (2018):674-680,
https://hdl.handle.net/21.15107/rcub_agrospace_4838 .