TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Gašić, Katarina
AU  - Blagojević, Nevena
AU  - Pulawska, Joanna
AU  - Obradović, Aleksa
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3302
AB  - Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia
EP  - 16
IS  - 1
SP  - 11
VL  - 137
DO  - 10.1007/s10658-013-0229-0
ER  - 

@article{
author = "Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Andjelka and Gašić, Katarina and Blagojević, Nevena and Pulawska, Joanna and Obradović, Aleksa",
year = "2013",
abstract = "Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia",
pages = "16-11",
number = "1",
volume = "137",
doi = "10.1007/s10658-013-0229-0"
}

Kuzmanović, N., Ivanović, M., Prokić, A., Gašić, K., Blagojević, N., Pulawska, J.,& Obradović, A.. (2013). Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 137(1), 11-16.
https://doi.org/10.1007/s10658-013-0229-0

Kuzmanović N, Ivanović M, Prokić A, Gašić K, Blagojević N, Pulawska J, Obradović A. Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology. 2013;137(1):11-16.
doi:10.1007/s10658-013-0229-0 .

Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Andjelka, Gašić, Katarina, Blagojević, Nevena, Pulawska, Joanna, Obradović, Aleksa, "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia" in European Journal of Plant Pathology, 137, no. 1 (2013):11-16,
https://doi.org/10.1007/s10658-013-0229-0 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Blagojević, Nevena
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2919
AB  - Raspberry and blackberry production, processing and export are main sources of incomes for the majority of farmers in particular areas of Serbia. However, profitability of the production is often compromised by disease outbreaks. Compared to fungal diseases, bacterial diseases are of less importance, but in years with favorable weather conditions can cause serious damage. This paper describes major bacterial diseases on raspberry and blackberry, such as bacterial blight caused by Pseudomonas syringae, crown gall caused by Agrobacterium tumefaciens and fire blight caused by Erwinia amylovora described as a pathogen of raspberry in North America. Beside bacteria, phytoplasmas can also be associated with raspberry and blackberry. The aim of this paper is to provide the knowledge about symptomatology and epidemiology of bacterial and phytoplasma diseases of raspberry and blackberry in order to facilitate accurate and timely diagnosis and adequate control strategy.
AB  - Proizvodnja, prerada i izvoz maline i kupine su u pojedinim regionima naše zemlje glavni izvor prihoda za većinu stanovništva. Međutim, profitabilnost proizvodnje često umanjuje pojava bolesti. U poređenju sa gljivičnim oboljenjima, bakterioze imaju manji značaj, ali pri vremenskim uslovima pogodnim za razvoj bolesti, mogu naneti ozbiljne štete. U radu su predstavljene najznačajnije bakterioze maline i kupine kao što su bakteriozna plamenjača, čiji je prouzrokovač Pseudomonas syringae, bakteriozni rak korena i izdanka, prouzrokovan bakterijom Agrobacterium tumefaciens, kao i bakteriozna plamenjača jabučastih voćaka prouzrokovana vrstom Erwinia amylovora, koja je opisana kao patogen maline u severnoj Americi. Osim bakterija, malinu i kupinu mogu parazitirati i fitoplazme. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja maline i kupine, kako bi se postavila pravilna i pravovremena dijagnoza i preduzele adekvatne mere zaštite.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial and phytoplasma diseases of raspberry and blackberry
T1  - Bakterioze i fitoplazmoze maline i kupine
EP  - 109
IS  - 2-3
SP  - 101
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2919
ER  - 

@article{
author = "Obradović, Aleksa and Blagojević, Nevena",
year = "2012",
abstract = "Raspberry and blackberry production, processing and export are main sources of incomes for the majority of farmers in particular areas of Serbia. However, profitability of the production is often compromised by disease outbreaks. Compared to fungal diseases, bacterial diseases are of less importance, but in years with favorable weather conditions can cause serious damage. This paper describes major bacterial diseases on raspberry and blackberry, such as bacterial blight caused by Pseudomonas syringae, crown gall caused by Agrobacterium tumefaciens and fire blight caused by Erwinia amylovora described as a pathogen of raspberry in North America. Beside bacteria, phytoplasmas can also be associated with raspberry and blackberry. The aim of this paper is to provide the knowledge about symptomatology and epidemiology of bacterial and phytoplasma diseases of raspberry and blackberry in order to facilitate accurate and timely diagnosis and adequate control strategy., Proizvodnja, prerada i izvoz maline i kupine su u pojedinim regionima naše zemlje glavni izvor prihoda za većinu stanovništva. Međutim, profitabilnost proizvodnje često umanjuje pojava bolesti. U poređenju sa gljivičnim oboljenjima, bakterioze imaju manji značaj, ali pri vremenskim uslovima pogodnim za razvoj bolesti, mogu naneti ozbiljne štete. U radu su predstavljene najznačajnije bakterioze maline i kupine kao što su bakteriozna plamenjača, čiji je prouzrokovač Pseudomonas syringae, bakteriozni rak korena i izdanka, prouzrokovan bakterijom Agrobacterium tumefaciens, kao i bakteriozna plamenjača jabučastih voćaka prouzrokovana vrstom Erwinia amylovora, koja je opisana kao patogen maline u severnoj Americi. Osim bakterija, malinu i kupinu mogu parazitirati i fitoplazme. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja maline i kupine, kako bi se postavila pravilna i pravovremena dijagnoza i preduzele adekvatne mere zaštite.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial and phytoplasma diseases of raspberry and blackberry, Bakterioze i fitoplazmoze maline i kupine",
pages = "109-101",
number = "2-3",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2919"
}

Obradović, A.,& Blagojević, N.. (2012). Bacterial and phytoplasma diseases of raspberry and blackberry. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 40(2-3), 101-109.
https://hdl.handle.net/21.15107/rcub_agrospace_2919

Obradović A, Blagojević N. Bacterial and phytoplasma diseases of raspberry and blackberry. in Biljni lekar. 2012;40(2-3):101-109.
https://hdl.handle.net/21.15107/rcub_agrospace_2919 .

Obradović, Aleksa, Blagojević, Nevena, "Bacterial and phytoplasma diseases of raspberry and blackberry" in Biljni lekar, 40, no. 2-3 (2012):101-109,
https://hdl.handle.net/21.15107/rcub_agrospace_2919 .