TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Mačukanović-Jocić, Marina
AU  - Radošević, Radenko
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6338
AB  - The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance. © 2023 by the authors.
T2  - Horticulturae
T2  - Horticulturae
T1  - The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040424
ER  - 

@article{
author = "Fotirić Akšić, Milica and Mačukanović-Jocić, Marina and Radošević, Radenko and Nedić, Nebojša and Gašić, Uroš and Tosti, Tomislav and Tešić, Živoslav and Meland, Mekjell",
year = "2023",
abstract = "The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance. © 2023 by the authors.",
journal = "Horticulturae, Horticulturae",
title = "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040424"
}

Fotirić Akšić, M., Mačukanović-Jocić, M., Radošević, R., Nedić, N., Gašić, U., Tosti, T., Tešić, Ž.,& Meland, M.. (2023). The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae, 9(4).
https://doi.org/10.3390/horticulturae9040424

Fotirić Akšić M, Mačukanović-Jocić M, Radošević R, Nedić N, Gašić U, Tosti T, Tešić Ž, Meland M. The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae. 2023;9(4).
doi:10.3390/horticulturae9040424 .

Fotirić Akšić, Milica, Mačukanović-Jocić, Marina, Radošević, Radenko, Nedić, Nebojša, Gašić, Uroš, Tosti, Tomislav, Tešić, Živoslav, Meland, Mekjell, "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora" in Horticulturae, 9, no. 4 (2023),
https://doi.org/10.3390/horticulturae9040424 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Tešić, Živoslav
AU  - Kalaba, Milica
AU  - Ćirić, Ivanka
AU  - Pezo, Lato
AU  - Lončar, Biljana
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Tosti, Tomislav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6340
AB  - The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds. © 2023 by the authors.
T2  - Horticulturae
T2  - Horticulturae
T1  - Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040477
ER  - 

@article{
author = "Fotirić Akšić, Milica and Tešić, Živoslav and Kalaba, Milica and Ćirić, Ivanka and Pezo, Lato and Lončar, Biljana and Gašić, Uroš and Dojčinović, Biljana and Tosti, Tomislav and Meland, Mekjell",
year = "2023",
abstract = "The aim of this study was to find the chemical parameters for the differentiation of plum cultivars grown along the fjord areas of Western Norway and Eastern Norway, having specific agroclimatic conditions. Chemical analysis of the fruits confirmed the contents of 13 quantified elements, 22 sugar compounds, 11 organic acids, 19 phenolic compounds, and antioxidant activity in 68 plum cultivars. Dominated contents were noted for nitrogen (with the maximum mean value of 3.11%), potassium (8055.80 mg/kg), and phosphorous (7878.88 mg/kg). Averagely, the highest level of sugars was determined for glucose (244.46 g/kg), fructose (197.92 g/kg), sucrose (208.25 g/kg), and sorbitol (98.02 g/kg), organic acids for malic acid (24.06 g/kg), and for polyphenol compounds were 5-O-caffeoylquinic acid (66.31 mg/kg), and rutin (58.06 mg/kg). Applied principal component analysis has been useful for distinguishing the plum cultivars from three areas in Norway where copper, iron, potassium, magnesium, manganese, and sodium; sucrose, ribose, maltose, and raffinose; p-hydroxybenzoic acid, rutin, ferulic acid, kaempferol 7-O-glucoside, p-coumaric acid, and 5-O-caffeoylquinic acid were the most influential. In regard to human health and future breeding work that will have the aim to produce functional food with high health-related compounds, the plum cultivar ‘Mallard’ should be underlined due to the high level of elements, ‘Valor’ due to high sugar content, ‘Helgøyplomme’ due to content of organic acids, and ‘Diamond’ due to the content of phenolic compounds. © 2023 by the authors.",
journal = "Horticulturae, Horticulturae",
title = "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040477"
}

Fotirić Akšić, M., Tešić, Ž., Kalaba, M., Ćirić, I., Pezo, L., Lončar, B., Gašić, U., Dojčinović, B., Tosti, T.,& Meland, M.. (2023). Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae, 9(4).
https://doi.org/10.3390/horticulturae9040477

Fotirić Akšić M, Tešić Ž, Kalaba M, Ćirić I, Pezo L, Lončar B, Gašić U, Dojčinović B, Tosti T, Meland M. Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway. in Horticulturae. 2023;9(4).
doi:10.3390/horticulturae9040477 .

Fotirić Akšić, Milica, Tešić, Živoslav, Kalaba, Milica, Ćirić, Ivanka, Pezo, Lato, Lončar, Biljana, Gašić, Uroš, Dojčinović, Biljana, Tosti, Tomislav, Meland, Mekjell, "Breakthrough Analysis of Chemical Composition and Applied Chemometrics of European Plum Cultivars Grown in Norway" in Horticulturae, 9, no. 4 (2023),
https://doi.org/10.3390/horticulturae9040477 . .

TY  - JOUR
AU  - Ćirković, Dušica
AU  - Matijašević, Saša
AU  - Ćirković, Bratislav
AU  - Laketić, Darko
AU  - Jovanović, Zoran
AU  - Kostić, Boban
AU  - Bešlić, Zoran
AU  - Sredojević, Milica
AU  - Tešić, Živoslav
AU  - Banjanac, Tijana
AU  - Gašić, Uroš
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6070
AB  - The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which produces quality wines of characteristic and unique tastes. In the agroecological conditions of the Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine produced from the variety Prokupac was examined. Four experimental treatments with different timings of manual defoliation were applied: early defoliation—treatment I, early defoliation— treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine was determined for the three treatments of defoliation and the control treatment. Additionally, a multivariate analysis was applied on the obtained results, together with already published data (grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained, while the treatments with early defoliation did not differ in relation to the control sample. Early defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins (TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.
PB  - MDPI
T2  - Horticulturae
T1  - Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)
IS  - 4
SP  - 296
VL  - 8
DO  - 10.3390/horticulturae8040296
ER  - 

@article{
author = "Ćirković, Dušica and Matijašević, Saša and Ćirković, Bratislav and Laketić, Darko and Jovanović, Zoran and Kostić, Boban and Bešlić, Zoran and Sredojević, Milica and Tešić, Živoslav and Banjanac, Tijana and Gašić, Uroš",
year = "2022",
abstract = "The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which produces quality wines of characteristic and unique tastes. In the agroecological conditions of the Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine produced from the variety Prokupac was examined. Four experimental treatments with different timings of manual defoliation were applied: early defoliation—treatment I, early defoliation— treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine was determined for the three treatments of defoliation and the control treatment. Additionally, a multivariate analysis was applied on the obtained results, together with already published data (grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained, while the treatments with early defoliation did not differ in relation to the control sample. Early defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins (TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)",
number = "4",
pages = "296",
volume = "8",
doi = "10.3390/horticulturae8040296"
}

Ćirković, D., Matijašević, S., Ćirković, B., Laketić, D., Jovanović, Z., Kostić, B., Bešlić, Z., Sredojević, M., Tešić, Ž., Banjanac, T.,& Gašić, U.. (2022). Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae
MDPI., 8(4), 296.
https://doi.org/10.3390/horticulturae8040296

Ćirković D, Matijašević S, Ćirković B, Laketić D, Jovanović Z, Kostić B, Bešlić Z, Sredojević M, Tešić Ž, Banjanac T, Gašić U. Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae. 2022;8(4):296.
doi:10.3390/horticulturae8040296 .

Ćirković, Dušica, Matijašević, Saša, Ćirković, Bratislav, Laketić, Darko, Jovanović, Zoran, Kostić, Boban, Bešlić, Zoran, Sredojević, Milica, Tešić, Živoslav, Banjanac, Tijana, Gašić, Uroš, "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)" in Horticulturae, 8, no. 4 (2022):296,
https://doi.org/10.3390/horticulturae8040296 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S2210803322000495
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6114
AB  - IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T2  - Journal of Herbal MedicineJournal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
SP  - 100580
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine, Journal of Herbal MedicineJournal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
pages = "100580",
volume = "34",
doi = "10.1016/j.hermed.2022.100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš M.
AU  - Lević, Steva
AU  - Stanojević, Sladjana P.
AU  - Barać, Miroljub B.
AU  - Tešić, Živoslav Lj.
AU  - Nedović, Viktor
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5882
AB  - The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.
PB  - MDPI AG
T2  - Biomolecules
T1  - Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties
IS  - 7
SP  - 965
VL  - 11
DO  - 10.3390/biom11070965
ER  - 

@article{
author = "Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Gašić, Uroš M. and Lević, Steva and Stanojević, Sladjana P. and Barać, Miroljub B. and Tešić, Živoslav Lj. and Nedović, Viktor and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of this research was phenolics and protein characterization and antioxidant properties evaluation of skimmed thermally treated goat’s milk powder enriched with different concentration of grape pomace seed extract (SE). The dominant phenolics in SE were phenolic acids, flavan–3-ols and procyanidins. Different electrophoretic techniques together with UHPLC-MS/MS analysis revealed the presence of phenolics-protein interactions in the samples, mainly procyanidins with whey protein/caseins complexes. Addition of SE into thermally treated goat’s milk significantly improved antioxidant properties of goat’s milk such as TAC, FRP, DPPH• and ABTS•+ scavenging activity. Gallic acid, catechin, and procyanidins mostly contributed to these activities. The schematic representation of phenolics–casein micelles interactions in thermally treated goat’s milk enriched with SE was given. The addition of SE into thermally treated goat’s milk can be a promising strategy in food waste recovery and to enhance the beneficial health effects of goat’s milk-based functional foods.",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties",
number = "7",
pages = "965",
volume = "11",
doi = "10.3390/biom11070965"
}

Milinčić, D. D., Kostić, A. Ž., Gašić, U. M., Lević, S., Stanojević, S. P., Barać, M. B., Tešić, Ž. Lj., Nedović, V.,& Pešić, M. B.. (2021). Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules
MDPI AG., 11(7), 965.
https://doi.org/10.3390/biom11070965

Milinčić DD, Kostić AŽ, Gašić UM, Lević S, Stanojević SP, Barać MB, Tešić ŽL, Nedović V, Pešić MB. Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties. in Biomolecules. 2021;11(7):965.
doi:10.3390/biom11070965 .

Milinčić, Danijel D., Kostić, Aleksandar Ž., Gašić, Uroš M., Lević, Steva, Stanojević, Sladjana P., Barać, Miroljub B., Tešić, Živoslav Lj., Nedović, Viktor, Pešić, Mirjana B., "Skimmed Goat’s Milk Powder Enriched with Grape Pomace Seed Extract: Phenolics and Protein Characterization and Antioxidant Properties" in Biomolecules, 11, no. 7 (2021):965,
https://doi.org/10.3390/biom11070965 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5886
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - MDPI AG
T2  - Antioxidants
T1  - Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen
IS  - 7
SP  - 1091
VL  - 10
DO  - 10.3390/antiox10071091
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones-28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen",
number = "7",
pages = "1091",
volume = "10",
doi = "10.3390/antiox10071091"
}

Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants
MDPI AG., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Phytochemical profile and antioxidant properties of bee-collected artichoke (Cynara scolymus) pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - JOUR
AU  - Kolarević, Tijana
AU  - Milinčić, Danijel D.
AU  - Vujović, Tatjana
AU  - Gašić, Uroš M.
AU  - Prokić, Ljiljana
AU  - Kostić, Aleksandar Ž.
AU  - Cerović, Radosav
AU  - Stanojević, Sladjana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5958
AB  - The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
PB  - MDPI
T2  - Horticulturae
T1  - Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
IS  - 11
SP  - 420
VL  - 7
DO  - 10.3390/horticulturae7110420
ER  - 

@article{
author = "Kolarević, Tijana and Milinčić, Danijel D. and Vujović, Tatjana and Gašić, Uroš M. and Prokić, Ljiljana and Kostić, Aleksandar Ž. and Cerović, Radosav and Stanojević, Sladjana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry",
number = "11",
pages = "420",
volume = "7",
doi = "10.3390/horticulturae7110420"
}

Kolarević, T., Milinčić, D. D., Vujović, T., Gašić, U. M., Prokić, L., Kostić, A. Ž., Cerović, R., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae
MDPI., 7(11), 420.
https://doi.org/10.3390/horticulturae7110420

Kolarević T, Milinčić DD, Vujović T, Gašić UM, Prokić L, Kostić AŽ, Cerović R, Stanojević SP, Tešić ŽL, Pešić MB. Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae. 2021;7(11):420.
doi:10.3390/horticulturae7110420 .

Kolarević, Tijana, Milinčić, Danijel D., Vujović, Tatjana, Gašić, Uroš M., Prokić, Ljiljana, Kostić, Aleksandar Ž., Cerović, Radosav, Stanojević, Sladjana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry" in Horticulturae, 7, no. 11 (2021):420,
https://doi.org/10.3390/horticulturae7110420 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Stanisavljević, Nemanja S.
AU  - Gašić, Uroš M.
AU  - Lević, Steva
AU  - Kojić, Milan O.
AU  - Tešić, Živoslav Lj.
AU  - Nedović, Viktor
AU  - Barać, Miroljub B.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5814
AB  - The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen
SP  - 129310
VL  - 351
DO  - 10.1016/j.foodchem.2021.129310
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Gašić, Uroš M. and Lević, Steva and Kojić, Milan O. and Tešić, Živoslav Lj. and Nedović, Viktor and Barać, Miroljub B. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen",
pages = "129310",
volume = "351",
doi = "10.1016/j.foodchem.2021.129310"
}

Kostić, A. Ž., Milinčić, D. D., Stanisavljević, N. S., Gašić, U. M., Lević, S., Kojić, M. O., Tešić, Ž. Lj., Nedović, V., Barać, M. B.,& Pešić, M. B.. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry
Elsevier Ltd., 351, 129310.
https://doi.org/10.1016/j.foodchem.2021.129310

Kostić AŽ, Milinčić DD, Stanisavljević NS, Gašić UM, Lević S, Kojić MO, Tešić ŽL, Nedović V, Barać MB, Pešić MB. Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry. 2021;351:129310.
doi:10.1016/j.foodchem.2021.129310 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Stanisavljević, Nemanja S., Gašić, Uroš M., Lević, Steva, Kojić, Milan O., Tešić, Živoslav Lj., Nedović, Viktor, Barać, Miroljub B., Pešić, Mirjana B., "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen" in Food Chemistry, 351 (2021):129310,
https://doi.org/10.1016/j.foodchem.2021.129310 . .

TY  - JOUR
AU  - Campos, Maria G.
AU  - Anjos, Ofélia
AU  - Chica, Manuel
AU  - Campoy, Pascual
AU  - Nozkova, Janka
AU  - Almaraz-Abarca, Norma
AU  - Barreto, Lidia M. R. C.
AU  - Nordi, João Carlos
AU  - Estevinho, Leticia M.
AU  - Pascoal, Ananias
AU  - Branco Paula, Vanessa
AU  - Chopina, Altino
AU  - Dias, Luis G.
AU  - Tešić, Živoslav Lj.
AU  - Mosić, Mirjana D.
AU  - Kostić, Aleksandar Ž.
AU  - Pešić, Mirjana B.
AU  - Milojković-Opsenica, Dušanka M.
AU  - Sickel, Wiebke
AU  - Ankenbrand, Markus J.
AU  - Grimmer, Gudrun
AU  - Steffan-Dewenter, Ingolf
AU  - Keller, Alexander
AU  - Förster, Frank
AU  - Tananaki, Chrysoula H.
AU  - Liolios, Vasilios
AU  - Kanelis, Dimitrios
AU  - Rodopoulou, Maria-Anna
AU  - Thrasyvoulou, Andreas
AU  - Paulo, Luísa
AU  - Kast, Christina
AU  - Lucchetti, Matteo A.
AU  - Glauser, Gaëtan
AU  - Lokutova, Olena
AU  - Bicudo de Almeida-Muradian, Ligia
AU  - Szczęsna, Teresa
AU  - Carreck, Norman L.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5930
AB  - “Bee pollen” is pollen collected from flowers by honey bees. It is used by the bees to nourish themselves, mainly by providing royal jelly and brood food, but it is also used for human nutrition. For the latter purpose, it is collected at the hive entrance as pellets that the bees bring to the hive. Bee pollen has diverse bioactivities, and thus has been used as a health food, and even as medication in some countries. In this paper, we provide standard methods for carrying out research on bee pollen. First, we introduce a method for the production and storage of bee pollen which assures quality of the product. Routine methods are then provided for the identification of the pollen’s floral sources, and determination of the more important quality criteria such as water content and content of proteins, carbohydrates, fatty acids, vitamins, alkaloids, phenolic and polyphenolic compounds. Finally, methods are described for the determination of some important bioactivities of bee pollen such as its antioxidant, anti-inflammatory, antimicrobial and antimutagenic properties. Métodos estándar Para la investigación del polen El "polen de abeja" es el polen recogido de las flores por las abejas melíferas. El polen de abeja es utilizado para nutrir a las propias abejas, principalmente para proporcionar jalea real y alimento para las crías, pero también se utiliza para la nutrición humana. Para este último fin, se recoge en la entrada de la colmena en forma de gránulos que las abejas llevan a la colmena. El polen de abeja tiene diversas bioactividades, por lo que se hautilizado como alimento para la salud, e incluso como medicamento en algunos países. En este artículo, proporcionamos métodos estándar para llevar a cabo investigaciones sobre el polen de abeja. En primer lugar, presentamos un método de producción y almacenamiento de polen de abeja que garantiza la calidad del producto. A continuación, se ofrecen métodos de rutina para la identificación de las fuentes florales del polen y la determinación de los criterios de calidad más importantes, como el contenido de agua y de proteínas, carbohidratos, ácidos grasos, vitaminas, alcaloides y compuestos fenólicos y polifenólicos. Por último, se describen métodos para la determinación de algunas bioactividades importantes del polen de abeja, como sus propiedades antioxidantes, antiinflamatorias, antimicrobianas y antimutagénicas.
PB  - Taylor and Francis Ltd.
T2  - Journal of Apicultural Research
T1  - Standard methods for pollen research
EP  - 109
IS  - 4
SP  - 1
VL  - 60
DO  - 10.1080/00218839.2021.1948240
ER  - 

@article{
author = "Campos, Maria G. and Anjos, Ofélia and Chica, Manuel and Campoy, Pascual and Nozkova, Janka and Almaraz-Abarca, Norma and Barreto, Lidia M. R. C. and Nordi, João Carlos and Estevinho, Leticia M. and Pascoal, Ananias and Branco Paula, Vanessa and Chopina, Altino and Dias, Luis G. and Tešić, Živoslav Lj. and Mosić, Mirjana D. and Kostić, Aleksandar Ž. and Pešić, Mirjana B. and Milojković-Opsenica, Dušanka M. and Sickel, Wiebke and Ankenbrand, Markus J. and Grimmer, Gudrun and Steffan-Dewenter, Ingolf and Keller, Alexander and Förster, Frank and Tananaki, Chrysoula H. and Liolios, Vasilios and Kanelis, Dimitrios and Rodopoulou, Maria-Anna and Thrasyvoulou, Andreas and Paulo, Luísa and Kast, Christina and Lucchetti, Matteo A. and Glauser, Gaëtan and Lokutova, Olena and Bicudo de Almeida-Muradian, Ligia and Szczęsna, Teresa and Carreck, Norman L.",
year = "2021",
abstract = "“Bee pollen” is pollen collected from flowers by honey bees. It is used by the bees to nourish themselves, mainly by providing royal jelly and brood food, but it is also used for human nutrition. For the latter purpose, it is collected at the hive entrance as pellets that the bees bring to the hive. Bee pollen has diverse bioactivities, and thus has been used as a health food, and even as medication in some countries. In this paper, we provide standard methods for carrying out research on bee pollen. First, we introduce a method for the production and storage of bee pollen which assures quality of the product. Routine methods are then provided for the identification of the pollen’s floral sources, and determination of the more important quality criteria such as water content and content of proteins, carbohydrates, fatty acids, vitamins, alkaloids, phenolic and polyphenolic compounds. Finally, methods are described for the determination of some important bioactivities of bee pollen such as its antioxidant, anti-inflammatory, antimicrobial and antimutagenic properties. Métodos estándar Para la investigación del polen El "polen de abeja" es el polen recogido de las flores por las abejas melíferas. El polen de abeja es utilizado para nutrir a las propias abejas, principalmente para proporcionar jalea real y alimento para las crías, pero también se utiliza para la nutrición humana. Para este último fin, se recoge en la entrada de la colmena en forma de gránulos que las abejas llevan a la colmena. El polen de abeja tiene diversas bioactividades, por lo que se hautilizado como alimento para la salud, e incluso como medicamento en algunos países. En este artículo, proporcionamos métodos estándar para llevar a cabo investigaciones sobre el polen de abeja. En primer lugar, presentamos un método de producción y almacenamiento de polen de abeja que garantiza la calidad del producto. A continuación, se ofrecen métodos de rutina para la identificación de las fuentes florales del polen y la determinación de los criterios de calidad más importantes, como el contenido de agua y de proteínas, carbohidratos, ácidos grasos, vitaminas, alcaloides y compuestos fenólicos y polifenólicos. Por último, se describen métodos para la determinación de algunas bioactividades importantes del polen de abeja, como sus propiedades antioxidantes, antiinflamatorias, antimicrobianas y antimutagénicas.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Apicultural Research",
title = "Standard methods for pollen research",
pages = "109-1",
number = "4",
volume = "60",
doi = "10.1080/00218839.2021.1948240"
}

Campos, M. G., Anjos, O., Chica, M., Campoy, P., Nozkova, J., Almaraz-Abarca, N., Barreto, L. M. R. C., Nordi, J. C., Estevinho, L. M., Pascoal, A., Branco Paula, V., Chopina, A., Dias, L. G., Tešić, Ž. Lj., Mosić, M. D., Kostić, A. Ž., Pešić, M. B., Milojković-Opsenica, D. M., Sickel, W., Ankenbrand, M. J., Grimmer, G., Steffan-Dewenter, I., Keller, A., Förster, F., Tananaki, C. H., Liolios, V., Kanelis, D., Rodopoulou, M., Thrasyvoulou, A., Paulo, L., Kast, C., Lucchetti, M. A., Glauser, G., Lokutova, O., Bicudo de Almeida-Muradian, L., Szczęsna, T.,& Carreck, N. L.. (2021). Standard methods for pollen research. in Journal of Apicultural Research
Taylor and Francis Ltd.., 60(4), 1-109.
https://doi.org/10.1080/00218839.2021.1948240

Campos MG, Anjos O, Chica M, Campoy P, Nozkova J, Almaraz-Abarca N, Barreto LMRC, Nordi JC, Estevinho LM, Pascoal A, Branco Paula V, Chopina A, Dias LG, Tešić ŽL, Mosić MD, Kostić AŽ, Pešić MB, Milojković-Opsenica DM, Sickel W, Ankenbrand MJ, Grimmer G, Steffan-Dewenter I, Keller A, Förster F, Tananaki CH, Liolios V, Kanelis D, Rodopoulou M, Thrasyvoulou A, Paulo L, Kast C, Lucchetti MA, Glauser G, Lokutova O, Bicudo de Almeida-Muradian L, Szczęsna T, Carreck NL. Standard methods for pollen research. in Journal of Apicultural Research. 2021;60(4):1-109.
doi:10.1080/00218839.2021.1948240 .

Campos, Maria G., Anjos, Ofélia, Chica, Manuel, Campoy, Pascual, Nozkova, Janka, Almaraz-Abarca, Norma, Barreto, Lidia M. R. C., Nordi, João Carlos, Estevinho, Leticia M., Pascoal, Ananias, Branco Paula, Vanessa, Chopina, Altino, Dias, Luis G., Tešić, Živoslav Lj., Mosić, Mirjana D., Kostić, Aleksandar Ž., Pešić, Mirjana B., Milojković-Opsenica, Dušanka M., Sickel, Wiebke, Ankenbrand, Markus J., Grimmer, Gudrun, Steffan-Dewenter, Ingolf, Keller, Alexander, Förster, Frank, Tananaki, Chrysoula H., Liolios, Vasilios, Kanelis, Dimitrios, Rodopoulou, Maria-Anna, Thrasyvoulou, Andreas, Paulo, Luísa, Kast, Christina, Lucchetti, Matteo A., Glauser, Gaëtan, Lokutova, Olena, Bicudo de Almeida-Muradian, Ligia, Szczęsna, Teresa, Carreck, Norman L., "Standard methods for pollen research" in Journal of Apicultural Research, 60, no. 4 (2021):1-109,
https://doi.org/10.1080/00218839.2021.1948240 . .

TY  - JOUR
AU  - Skočajić, Dragana
AU  - Gašić, Uroš
AU  - Dabić Zagorac, Dragana
AU  - Nešić, Marija
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
AU  - Fotirić-Akšić, Milica
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6005
AB  - The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars (‘Amanogawa’, ‘Kanzan’, and ‘Kiku-shidare-zakura’) and commercial rootstocks. Prunus avium, Prunus ‘Colt’, Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis ‘Pyrodwarf’ were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with ‘Amanogawa’ and ‘Kiku-shidare-zakura’ the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.
PB  - MDPI
T2  - Plants
T1  - Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries
IS  - 12
SP  - 2822
VL  - 10
DO  - 10.3390/plants10122822
ER  - 

@article{
author = "Skočajić, Dragana and Gašić, Uroš and Dabić Zagorac, Dragana and Nešić, Marija and Tešić, Živoslav and Meland, Mekjell and Fotirić-Akšić, Milica",
year = "2021",
abstract = "The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars (‘Amanogawa’, ‘Kanzan’, and ‘Kiku-shidare-zakura’) and commercial rootstocks. Prunus avium, Prunus ‘Colt’, Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis ‘Pyrodwarf’ were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with ‘Amanogawa’ and ‘Kiku-shidare-zakura’ the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.",
publisher = "MDPI",
journal = "Plants",
title = "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries",
number = "12",
pages = "2822",
volume = "10",
doi = "10.3390/plants10122822"
}

Skočajić, D., Gašić, U., Dabić Zagorac, D., Nešić, M., Tešić, Ž., Meland, M.,& Fotirić-Akšić, M.. (2021). Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries. in Plants
MDPI., 10(12), 2822.
https://doi.org/10.3390/plants10122822

Skočajić D, Gašić U, Dabić Zagorac D, Nešić M, Tešić Ž, Meland M, Fotirić-Akšić M. Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries. in Plants. 2021;10(12):2822.
doi:10.3390/plants10122822 .

Skočajić, Dragana, Gašić, Uroš, Dabić Zagorac, Dragana, Nešić, Marija, Tešić, Živoslav, Meland, Mekjell, Fotirić-Akšić, Milica, "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries" in Plants, 10, no. 12 (2021):2822,
https://doi.org/10.3390/plants10122822 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5911
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
EP  - 25829
IS  - 42
SP  - 25816
VL  - 11
DO  - 10.1039/d1ra04250e
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
pages = "25829-25816",
number = "42",
volume = "11",
doi = "10.1039/d1ra04250e"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/d1ra04250e

Nešović M, Gašić U, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić Ž. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/d1ra04250e .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/d1ra04250e . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Špirović Trifunović, Bojana D.
AU  - Stanojević, Sladjana P.
AU  - Lević, Steva
AU  - Nedić, Nebojša
AU  - Nedović, Viktor
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5823
AB  - The aim of this study was to examine nutritional (total lipid, carbohydrate, protein and ash content, fatty acids and protein profile) and techno-functional properties of monofloral bee-collected sunflower pollen (Helianthus annuus L.). The content of water, total protein, carbohydrate, lipid and ash was 24.99, 14.36, 82.01, 1.62 and 2.01 g/100g DW, respectively, with the total energy value of 400.06 kcal/100 g DW. The fatty acid profile revealed the presence of five fatty acids with the stearic acid as dominant one (31.4%) followed by α-linoleic (20.7%), pentadecanoic (18.2%), heneicosanoic (17.1%) and palmitic (12.5%) acids. FTIR analysis of bee pollen grains confirmed the presence of the main pollen chemical constituents such as proteins, water, carbohydrates and lipids, but also the presence of sporopollenin and polyphenols. Raman spectroscopy analysis indicated that the surface of pollen grains was rich in carotenoids. Low protein (3.64 g/100g DW) but high carbohydrate (77.09 g/100 g DW) solubility of bee pollen was observed. The good emulsifying properties (ESI, 19.98 min; EAI, 80.54 m2/g) and excellent oil (2.43 g/g DW) but poor water (0.87 g/g DW) absorption capacities were also registered. On the other hand, the sunflower bee pollen did not show foaming properties. These findings indicate to its applicability as useful nutritional, lipophilic and anti-foaming food ingredients.
PB  - United Arab Emirates University
T2  - Emirates Journal of Food and Agriculture
T1  - Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen
EP  - 777
IS  - 11
SP  - 768
VL  - 32
DO  - 10.9755/ejfa.2020.v32.i11.2188
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Špirović Trifunović, Bojana D. and Stanojević, Sladjana P. and Lević, Steva and Nedić, Nebojša and Nedović, Viktor and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2020",
abstract = "The aim of this study was to examine nutritional (total lipid, carbohydrate, protein and ash content, fatty acids and protein profile) and techno-functional properties of monofloral bee-collected sunflower pollen (Helianthus annuus L.). The content of water, total protein, carbohydrate, lipid and ash was 24.99, 14.36, 82.01, 1.62 and 2.01 g/100g DW, respectively, with the total energy value of 400.06 kcal/100 g DW. The fatty acid profile revealed the presence of five fatty acids with the stearic acid as dominant one (31.4%) followed by α-linoleic (20.7%), pentadecanoic (18.2%), heneicosanoic (17.1%) and palmitic (12.5%) acids. FTIR analysis of bee pollen grains confirmed the presence of the main pollen chemical constituents such as proteins, water, carbohydrates and lipids, but also the presence of sporopollenin and polyphenols. Raman spectroscopy analysis indicated that the surface of pollen grains was rich in carotenoids. Low protein (3.64 g/100g DW) but high carbohydrate (77.09 g/100 g DW) solubility of bee pollen was observed. The good emulsifying properties (ESI, 19.98 min; EAI, 80.54 m2/g) and excellent oil (2.43 g/g DW) but poor water (0.87 g/g DW) absorption capacities were also registered. On the other hand, the sunflower bee pollen did not show foaming properties. These findings indicate to its applicability as useful nutritional, lipophilic and anti-foaming food ingredients.",
publisher = "United Arab Emirates University",
journal = "Emirates Journal of Food and Agriculture",
title = "Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen",
pages = "777-768",
number = "11",
volume = "32",
doi = "10.9755/ejfa.2020.v32.i11.2188"
}

Kostić, A. Ž., Milinčić, D. D., Špirović Trifunović, B. D., Stanojević, S. P., Lević, S., Nedić, N., Nedović, V., Tešić, Ž. Lj.,& Pešić, M. B.. (2020). Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen. in Emirates Journal of Food and Agriculture
United Arab Emirates University., 32(11), 768-777.
https://doi.org/10.9755/ejfa.2020.v32.i11.2188

Kostić AŽ, Milinčić DD, Špirović Trifunović BD, Stanojević SP, Lević S, Nedić N, Nedović V, Tešić ŽL, Pešić MB. Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen. in Emirates Journal of Food and Agriculture. 2020;32(11):768-777.
doi:10.9755/ejfa.2020.v32.i11.2188 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Špirović Trifunović, Bojana D., Stanojević, Sladjana P., Lević, Steva, Nedić, Nebojša, Nedović, Viktor, Tešić, Živoslav Lj., Pešić, Mirjana B., "Nutritional and techno-functional properties of monofloral bee-collected sunflower (Helianthus annuus L.) pollen" in Emirates Journal of Food and Agriculture, 32, no. 11 (2020):768-777,
https://doi.org/10.9755/ejfa.2020.v32.i11.2188 . .

TY  - CONF
AU  - Kostić, Aleksandar
AU  - Dojčinović, Biljana
AU  - Nedić, Nebojša
AU  - Milincić, Danijel D.
AU  - Pantelić, Nebojša
AU  - Stanojević, Sladjana
AU  - Barać, Miroljub
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5434
AB  - Pollen (floral or bee-collected) can be used as a good source of different nutrients including several important biogenic elements (minerals). The aim of this study was to determine content of selected biogenic elements (phosphorous, potassium, sulphur, calcium, magnesium, iron and zinc) in monofloral bee-collected pollen originated from sunflower (Helianthus annuus L.) plants grown in Serbia and to estimate its nutritional value regarding to elements content. In that purpose ICP-OES analytical method was applied. According to obtained results the examined pollen sample contains above mentioned elements in following quantities: P – 3523.98 mg/kg; K – 2869.13 mg/kg; S – 1951.74 mg/kg; Ca – 1919.20 mg/kg; Mg – 654.36 mg/kg; Fe –83.55 mg/kg; Zn – 45.92 mg/kg of dry weight. Based on recommended daily intakes for iron, phosphorus and zinc application of pollen, as food or food ingredient, will cover meaningful part of human needs for these three important elements.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Content and nutritional value of selected biogenic elements in monofloral sunflower bee-collected pollen from Serbia
EP  - 217
SP  - 211
VL  - 78
DO  - 10.1007/978-3-030-40049-1_27
ER  - 

@conference{
author = "Kostić, Aleksandar and Dojčinović, Biljana and Nedić, Nebojša and Milincić, Danijel D. and Pantelić, Nebojša and Stanojević, Sladjana and Barać, Miroljub and Tešić, Živoslav and Pešić, Mirjana",
year = "2020",
abstract = "Pollen (floral or bee-collected) can be used as a good source of different nutrients including several important biogenic elements (minerals). The aim of this study was to determine content of selected biogenic elements (phosphorous, potassium, sulphur, calcium, magnesium, iron and zinc) in monofloral bee-collected pollen originated from sunflower (Helianthus annuus L.) plants grown in Serbia and to estimate its nutritional value regarding to elements content. In that purpose ICP-OES analytical method was applied. According to obtained results the examined pollen sample contains above mentioned elements in following quantities: P – 3523.98 mg/kg; K – 2869.13 mg/kg; S – 1951.74 mg/kg; Ca – 1919.20 mg/kg; Mg – 654.36 mg/kg; Fe –83.55 mg/kg; Zn – 45.92 mg/kg of dry weight. Based on recommended daily intakes for iron, phosphorus and zinc application of pollen, as food or food ingredient, will cover meaningful part of human needs for these three important elements.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Content and nutritional value of selected biogenic elements in monofloral sunflower bee-collected pollen from Serbia",
pages = "217-211",
volume = "78",
doi = "10.1007/978-3-030-40049-1_27"
}

Kostić, A., Dojčinović, B., Nedić, N., Milincić, D. D., Pantelić, N., Stanojević, S., Barać, M., Tešić, Ž.,& Pešić, M.. (2020). Content and nutritional value of selected biogenic elements in monofloral sunflower bee-collected pollen from Serbia. in IFMBE Proceedings
Springer., 78, 211-217.
https://doi.org/10.1007/978-3-030-40049-1_27

Kostić A, Dojčinović B, Nedić N, Milincić DD, Pantelić N, Stanojević S, Barać M, Tešić Ž, Pešić M. Content and nutritional value of selected biogenic elements in monofloral sunflower bee-collected pollen from Serbia. in IFMBE Proceedings. 2020;78:211-217.
doi:10.1007/978-3-030-40049-1_27 .

Kostić, Aleksandar, Dojčinović, Biljana, Nedić, Nebojša, Milincić, Danijel D., Pantelić, Nebojša, Stanojević, Sladjana, Barać, Miroljub, Tešić, Živoslav, Pešić, Mirjana, "Content and nutritional value of selected biogenic elements in monofloral sunflower bee-collected pollen from Serbia" in IFMBE Proceedings, 78 (2020):211-217,
https://doi.org/10.1007/978-3-030-40049-1_27 . .

TY  - CONF
AU  - Radović, Mirjana
AU  - Milatović, Dragan
AU  - Tešić, Živoslav
AU  - Dabić Zagorac, Dragana
AU  - Zec, Gordan
AU  - Boškov, Đorđe
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5789
AB  - Chemical and antioxidant properties of fruits of three plum cultivars (‘Čacanska Rana’,
‘Čacanska Lepotica’ and ‘Čacanska Najbolja’) grafted on four rootstocks (Myrobalan, ‘Pixy’,
‘Fereley’ and ‘St. Julien A’) were studied in the region of Belgrade (Serbia). Chemical
composition of plum fruits was found to be more cultivar- then rootstock-dependent. The
average values for soluble solids, total sugars, inverted sugars, sucrose, and total acids content
in cultivar/rootstocks combinations ranged from 11.7 to 14.2%, from 9.5 to 11.5%, from 6.6
to 7.6%, from 2.7 to 3.9%, and from 0.71 to1.11%, respectively. The contents of soluble
solids and total sugars were the highest in fruits of ‘Čacanska Najbolja’, while the lowest
contents were found in fruits of ‘Čacanska Rana’ cultivar. Total phenolic content (TPC) in the
skin and flesh of plums was in the range from 4.44 to 15.93 mg GAE g-1, and from 0.38 to
0.86 mg GAE g-1 respectively. TPC in the skin was 6–20 times higher than in the flesh of the
same cultivar/rootstock combinations. Differences in TPC among rootstocks were not
statistically significant, but significant differences among cultivars were found. Radical scavenging
activity (RSA) in the skin and flesh ranged from 39.08 to 78.49 μmol TE g-1, and
from 10.40 to 16.97 μmol TE g-1 respectively. Significant differences in RSA were found
among cultivars and cultivar/rootstock combinations, whereas differences between rootstocks
were not significant. The highest RSA was found in fruits of ‘Čacanska Najbolja’ cultivar.
PB  - Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina
C3  - XI International Scientific Agricultural Symposium “Agrosym 2020”
T1  - Chemical composition and antioxidant activity of fruits of three plum cultivars grafted on four rootstocks
EP  - 319
SP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5789
ER  - 

@conference{
author = "Radović, Mirjana and Milatović, Dragan and Tešić, Živoslav and Dabić Zagorac, Dragana and Zec, Gordan and Boškov, Đorđe",
year = "2020",
abstract = "Chemical and antioxidant properties of fruits of three plum cultivars (‘Čacanska Rana’,
‘Čacanska Lepotica’ and ‘Čacanska Najbolja’) grafted on four rootstocks (Myrobalan, ‘Pixy’,
‘Fereley’ and ‘St. Julien A’) were studied in the region of Belgrade (Serbia). Chemical
composition of plum fruits was found to be more cultivar- then rootstock-dependent. The
average values for soluble solids, total sugars, inverted sugars, sucrose, and total acids content
in cultivar/rootstocks combinations ranged from 11.7 to 14.2%, from 9.5 to 11.5%, from 6.6
to 7.6%, from 2.7 to 3.9%, and from 0.71 to1.11%, respectively. The contents of soluble
solids and total sugars were the highest in fruits of ‘Čacanska Najbolja’, while the lowest
contents were found in fruits of ‘Čacanska Rana’ cultivar. Total phenolic content (TPC) in the
skin and flesh of plums was in the range from 4.44 to 15.93 mg GAE g-1, and from 0.38 to
0.86 mg GAE g-1 respectively. TPC in the skin was 6–20 times higher than in the flesh of the
same cultivar/rootstock combinations. Differences in TPC among rootstocks were not
statistically significant, but significant differences among cultivars were found. Radical scavenging
activity (RSA) in the skin and flesh ranged from 39.08 to 78.49 μmol TE g-1, and
from 10.40 to 16.97 μmol TE g-1 respectively. Significant differences in RSA were found
among cultivars and cultivar/rootstock combinations, whereas differences between rootstocks
were not significant. The highest RSA was found in fruits of ‘Čacanska Najbolja’ cultivar.",
publisher = "Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina",
journal = "XI International Scientific Agricultural Symposium “Agrosym 2020”",
title = "Chemical composition and antioxidant activity of fruits of three plum cultivars grafted on four rootstocks",
pages = "319-311",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5789"
}

Radović, M., Milatović, D., Tešić, Ž., Dabić Zagorac, D., Zec, G.,& Boškov, Đ.. (2020). Chemical composition and antioxidant activity of fruits of three plum cultivars grafted on four rootstocks. in XI International Scientific Agricultural Symposium “Agrosym 2020”
Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina., 311-319.
https://hdl.handle.net/21.15107/rcub_agrospace_5789

Radović M, Milatović D, Tešić Ž, Dabić Zagorac D, Zec G, Boškov Đ. Chemical composition and antioxidant activity of fruits of three plum cultivars grafted on four rootstocks. in XI International Scientific Agricultural Symposium “Agrosym 2020”. 2020;:311-319.
https://hdl.handle.net/21.15107/rcub_agrospace_5789 .

Radović, Mirjana, Milatović, Dragan, Tešić, Živoslav, Dabić Zagorac, Dragana, Zec, Gordan, Boškov, Đorđe, "Chemical composition and antioxidant activity of fruits of three plum cultivars grafted on four rootstocks" in XI International Scientific Agricultural Symposium “Agrosym 2020” (2020):311-319,
https://hdl.handle.net/21.15107/rcub_agrospace_5789 .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milincić, Danijel D.
AU  - Barać, Miroljub
AU  - Ali Shariati, Mohammad
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5336
AB  - Pollen is recognized as an excellent dietary supplement for human nutrition, which is why it can be found in different forms on the market (granules, capsules, tablets, pellets, and powders). But, the digestibility of pollen's nutrients is strongly affected by the presence of a pollen shell, which can decrease the bioavailability of nutrients by 50% and more. Since consumers have become more aware of the benefits of a healthy diet and the necessity to improve pollen digestibility, different pollen-based functional food products have been developed and extensive studies were done to estimate the beneficial effects of pollen-based feed on animal growth, health, and rigor mortise stage. Considering the positive effects of pollen nutrients and phytometabolites on human and animal health, the aim of this paper was to give an overview of recent achievements in the application of pollen in the formulation of functional food and animal diets. Special attention was paid to the effects of pollen's addition on the nutritional, functional, techno-functional, and sensory properties of the new formulated food products. Anti-nutritional properties of pollen were also discussed. This review points out the benefits of pollen addition to food and feed and the possible directions in the further development of functional food and feed for the wellbeing of everyone.
PB  - MDPI, BASEL
T2  - Biomolecules
T1  - The Application of Pollen as a Functional Food and Feed Ingredient-The Present and Perspectives
IS  - 1
VL  - 10
DO  - 10.3390/biom10010084
ER  - 

@article{
author = "Kostić, Aleksandar and Milincić, Danijel D. and Barać, Miroljub and Ali Shariati, Mohammad and Tešić, Živoslav and Pešić, Mirjana",
year = "2020",
abstract = "Pollen is recognized as an excellent dietary supplement for human nutrition, which is why it can be found in different forms on the market (granules, capsules, tablets, pellets, and powders). But, the digestibility of pollen's nutrients is strongly affected by the presence of a pollen shell, which can decrease the bioavailability of nutrients by 50% and more. Since consumers have become more aware of the benefits of a healthy diet and the necessity to improve pollen digestibility, different pollen-based functional food products have been developed and extensive studies were done to estimate the beneficial effects of pollen-based feed on animal growth, health, and rigor mortise stage. Considering the positive effects of pollen nutrients and phytometabolites on human and animal health, the aim of this paper was to give an overview of recent achievements in the application of pollen in the formulation of functional food and animal diets. Special attention was paid to the effects of pollen's addition on the nutritional, functional, techno-functional, and sensory properties of the new formulated food products. Anti-nutritional properties of pollen were also discussed. This review points out the benefits of pollen addition to food and feed and the possible directions in the further development of functional food and feed for the wellbeing of everyone.",
publisher = "MDPI, BASEL",
journal = "Biomolecules",
title = "The Application of Pollen as a Functional Food and Feed Ingredient-The Present and Perspectives",
number = "1",
volume = "10",
doi = "10.3390/biom10010084"
}

Kostić, A., Milincić, D. D., Barać, M., Ali Shariati, M., Tešić, Ž.,& Pešić, M.. (2020). The Application of Pollen as a Functional Food and Feed Ingredient-The Present and Perspectives. in Biomolecules
MDPI, BASEL., 10(1).
https://doi.org/10.3390/biom10010084

Kostić A, Milincić DD, Barać M, Ali Shariati M, Tešić Ž, Pešić M. The Application of Pollen as a Functional Food and Feed Ingredient-The Present and Perspectives. in Biomolecules. 2020;10(1).
doi:10.3390/biom10010084 .

Kostić, Aleksandar, Milincić, Danijel D., Barać, Miroljub, Ali Shariati, Mohammad, Tešić, Živoslav, Pešić, Mirjana, "The Application of Pollen as a Functional Food and Feed Ingredient-The Present and Perspectives" in Biomolecules, 10, no. 1 (2020),
https://doi.org/10.3390/biom10010084 . .

TY  - JOUR
AU  - Stikić, Radmila
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar
AU  - Jovanović, Zorica
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5367
AB  - BACKGROUND:  and objectives Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.
PB  - Wiley, Hoboken
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
EP  - 633
IS  - 3
SP  - 626
VL  - 97
DO  - 10.1002/cche.10278
ER  - 

@article{
author = "Stikić, Radmila and Milincić, Danijel D. and Kostić, Aleksandar and Jovanović, Zorica and Gašić, Uroš M. and Tešić, Živoslav and Djordjević, Nataša Z. and Savić, Sladjana and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "BACKGROUND:  and objectives Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.",
publisher = "Wiley, Hoboken",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
pages = "633-626",
number = "3",
volume = "97",
doi = "10.1002/cche.10278"
}

Stikić, R., Milincić, D. D., Kostić, A., Jovanović, Z., Gašić, U. M., Tešić, Ž., Djordjević, N. Z., Savić, S., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry
Wiley, Hoboken., 97(3), 626-633.
https://doi.org/10.1002/cche.10278

Stikić R, Milincić DD, Kostić A, Jovanović Z, Gašić UM, Tešić Ž, Djordjević NZ, Savić S, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):626-633.
doi:10.1002/cche.10278 .

Stikić, Radmila, Milincić, Danijel D., Kostić, Aleksandar, Jovanović, Zorica, Gašić, Uroš M., Tešić, Živoslav, Djordjević, Nataša Z., Savić, Sladjana, Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):626-633,
https://doi.org/10.1002/cche.10278 . .

TY  - JOUR
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar
AU  - Špirović-Trifunović, Bojana
AU  - Tešić, Živoslav
AU  - Tosti, Tomislav B.
AU  - Dramicanin, Aleksandra M.
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5392
AB  - The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly w-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value
EP  - 319
IS  - 3
SP  - 305
VL  - 85
DO  - 10.2298/JSC190713117M
ER  - 

@article{
author = "Milincić, Danijel D. and Kostić, Aleksandar and Špirović-Trifunović, Bojana and Tešić, Živoslav and Tosti, Tomislav B. and Dramicanin, Aleksandra M. and Barać, Miroljub and Pešić, Mirjana",
year = "2020",
abstract = "The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly w-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value",
pages = "319-305",
number = "3",
volume = "85",
doi = "10.2298/JSC190713117M"
}

Milincić, D. D., Kostić, A., Špirović-Trifunović, B., Tešić, Ž., Tosti, T. B., Dramicanin, A. M., Barać, M.,& Pešić, M.. (2020). Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 85(3), 305-319.
https://doi.org/10.2298/JSC190713117M

Milincić DD, Kostić A, Špirović-Trifunović B, Tešić Ž, Tosti TB, Dramicanin AM, Barać M, Pešić M. Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2020;85(3):305-319.
doi:10.2298/JSC190713117M .

Milincić, Danijel D., Kostić, Aleksandar, Špirović-Trifunović, Bojana, Tešić, Živoslav, Tosti, Tomislav B., Dramicanin, Aleksandra M., Barać, Miroljub, Pešić, Mirjana, "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 85, no. 3 (2020):305-319,
https://doi.org/10.2298/JSC190713117M . .

TY  - JOUR
AU  - Dabić-Zagorac, Dragana
AU  - Fotirić-Akšić, Milica
AU  - Glavnik, Vesna
AU  - Gašić, Uroš M.
AU  - Vovk, Irena
AU  - Tešić, Živoslav
AU  - Natić, Maja
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5408
AB  - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimerwere identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
EP  - 1439
IS  - 8
SP  - 1431
VL  - 43
DO  - 10.1002/jssc.201901271
ER  - 

@article{
author = "Dabić-Zagorac, Dragana and Fotirić-Akšić, Milica and Glavnik, Vesna and Gašić, Uroš M. and Vovk, Irena and Tešić, Živoslav and Natić, Maja",
year = "2020",
abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimerwere identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components' scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
pages = "1439-1431",
number = "8",
volume = "43",
doi = "10.1002/jssc.201901271"
}

Dabić-Zagorac, D., Fotirić-Akšić, M., Glavnik, V., Gašić, U. M., Vovk, I., Tešić, Ž.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science
Wiley-V C H Verlag Gmbh, Weinheim., 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271

Dabić-Zagorac D, Fotirić-Akšić M, Glavnik V, Gašić UM, Vovk I, Tešić Ž, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .

Dabić-Zagorac, Dragana, Fotirić-Akšić, Milica, Glavnik, Vesna, Gašić, Uroš M., Vovk, Irena, Tešić, Živoslav, Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .

TY  - CONF
AU  - Fotirić-Akšić, Milica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5439
AB  - Apples are an important source of bioactive compounds, especially mineral nutrients. Minerals (both macro- and micro-elements) are responsible for the functioning of the human body, and involved in the metabolism and production of carbohydrates, lipids, proteins, vitamins and enzymes. The objective of this study was to determine and compare the content of some minerals (K, P, Ca, Mg and Fe) of two different apple cultivars ('Red Aroma Orelind' and 'Discovery'), both in peel and pulp, grown in organic and integrated production systems in western Norway in 2015. Samples of fruits were prepared by microwave digestion using an Ethos 1 microwave system. All analyses were performed in triplicate on a Thermo Scientific iCAP 6500 Duo ICP. The results showed that potassium was found in the largest quantity in both apple cultivars and in both management systems and average concentration was 944.3 µg g-1 of fresh weight (FW). Calcium was in second place with average of 180.6 µg g-1 FW. Both apple cultivars had largest amounts of both K and P (in peel and flesh) from organic production compared to fruits coming from the integrated production system. The cultivar 'Red Aroma Orelind' had a significantly higher level of Fe in organic fruits compared to integrated. The present study showed that the highest values of K/Ca, (K+Mg)/Ca and Mg/Ca were obtained from the cultivar 'Discovery' under organic production (both in peel and flesh). Regarding significantly higher concentrations of P, K, Mg, Fe and balanced mineral ratios in organic apples, it can be concluded that those fruits have higher quality, and are at lower risk of bitter pit when stored.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Evaluation of fruit mineral contents of two apple cultivars grown in organic and integrated production systems
EP  - 65
SP  - 59
VL  - 1281
DO  - 10.17660/ActaHortic.2020.1281.10
ER  - 

@conference{
author = "Fotirić-Akšić, Milica and Mutić, Jelena and Tešić, Živoslav and Meland, Mekjell",
year = "2020",
abstract = "Apples are an important source of bioactive compounds, especially mineral nutrients. Minerals (both macro- and micro-elements) are responsible for the functioning of the human body, and involved in the metabolism and production of carbohydrates, lipids, proteins, vitamins and enzymes. The objective of this study was to determine and compare the content of some minerals (K, P, Ca, Mg and Fe) of two different apple cultivars ('Red Aroma Orelind' and 'Discovery'), both in peel and pulp, grown in organic and integrated production systems in western Norway in 2015. Samples of fruits were prepared by microwave digestion using an Ethos 1 microwave system. All analyses were performed in triplicate on a Thermo Scientific iCAP 6500 Duo ICP. The results showed that potassium was found in the largest quantity in both apple cultivars and in both management systems and average concentration was 944.3 µg g-1 of fresh weight (FW). Calcium was in second place with average of 180.6 µg g-1 FW. Both apple cultivars had largest amounts of both K and P (in peel and flesh) from organic production compared to fruits coming from the integrated production system. The cultivar 'Red Aroma Orelind' had a significantly higher level of Fe in organic fruits compared to integrated. The present study showed that the highest values of K/Ca, (K+Mg)/Ca and Mg/Ca were obtained from the cultivar 'Discovery' under organic production (both in peel and flesh). Regarding significantly higher concentrations of P, K, Mg, Fe and balanced mineral ratios in organic apples, it can be concluded that those fruits have higher quality, and are at lower risk of bitter pit when stored.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Evaluation of fruit mineral contents of two apple cultivars grown in organic and integrated production systems",
pages = "65-59",
volume = "1281",
doi = "10.17660/ActaHortic.2020.1281.10"
}

Fotirić-Akšić, M., Mutić, J., Tešić, Ž.,& Meland, M.. (2020). Evaluation of fruit mineral contents of two apple cultivars grown in organic and integrated production systems. in Acta Horticulturae
International Society for Horticultural Science., 1281, 59-65.
https://doi.org/10.17660/ActaHortic.2020.1281.10

Fotirić-Akšić M, Mutić J, Tešić Ž, Meland M. Evaluation of fruit mineral contents of two apple cultivars grown in organic and integrated production systems. in Acta Horticulturae. 2020;1281:59-65.
doi:10.17660/ActaHortic.2020.1281.10 .

Fotirić-Akšić, Milica, Mutić, Jelena, Tešić, Živoslav, Meland, Mekjell, "Evaluation of fruit mineral contents of two apple cultivars grown in organic and integrated production systems" in Acta Horticulturae, 1281 (2020):59-65,
https://doi.org/10.17660/ActaHortic.2020.1281.10 . .

TY  - JOUR
AU  - Suković, Danijela
AU  - Knežević, Bojana
AU  - Gašić, Uroš M.
AU  - Sredojević, Milica
AU  - Ćirić, Ivanka
AU  - Todić, Slavica
AU  - Mutić, Jelena
AU  - Tešić, Živoslav
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5391
AB  - Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.
PB  - MDPI, BASEL
T2  - Foods
T1  - Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro
IS  - 2
VL  - 9
DO  - 10.3390/foods9020138
ER  - 

@article{
author = "Suković, Danijela and Knežević, Bojana and Gašić, Uroš M. and Sredojević, Milica and Ćirić, Ivanka and Todić, Slavica and Mutić, Jelena and Tešić, Živoslav",
year = "2020",
abstract = "Vranac, an old autochthonous red grapevine variety of Montenegro, was first mentioned in a historical document published in the 15th century. As currently the study of indigenous varieties is of particular importance, the subject of this work was detailed characterization of phenolic compounds in the autochthonous grapevine variety Vranac, from the Montenegrin Podgorica subregion. Phenolic profiles of leaves, berries (skin, seeds, and pulp were examined separately) and young monovarietal wine were determined using ultra-high performance liquid chromatography (UHPLC) with linear trap quadrupole (LTQ)-Orbitrap XL mass spectrometry (MS). Total phenolic content (TPC) and radical scavenging activity (RSA) were higher for the grape seeds extracts, followed by extracts of grape skins and pulps. As expected, the total anthocyanin content (TAC) was higher in grape skin than in wine. A total of one hundred twenty nine compounds (forty two phenolic acids and their derivatives, twenty three flavan-3-ols, twenty one flavanols, five stilbenes and thirty eight anthocyanins) were identified in the investigated extracts. To our best knowledge, this is the first report of tentative identification of (epi)catechin 3-O-coumarate in grape seed and chalcan-flavan 3-ol dimers in wine and grape seed.",
publisher = "MDPI, BASEL",
journal = "Foods",
title = "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro",
number = "2",
volume = "9",
doi = "10.3390/foods9020138"
}

Suković, D., Knežević, B., Gašić, U. M., Sredojević, M., Ćirić, I., Todić, S., Mutić, J.,& Tešić, Ž.. (2020). Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro. in Foods
MDPI, BASEL., 9(2).
https://doi.org/10.3390/foods9020138

Suković D, Knežević B, Gašić UM, Sredojević M, Ćirić I, Todić S, Mutić J, Tešić Ž. Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro. in Foods. 2020;9(2).
doi:10.3390/foods9020138 .

Suković, Danijela, Knežević, Bojana, Gašić, Uroš M., Sredojević, Milica, Ćirić, Ivanka, Todić, Slavica, Mutić, Jelena, Tešić, Živoslav, "Phenolic Profiles of Leaves, Grapes and Wine of Grapevine Variety Vranac (Vitis vinifera L.) from Montenegro" in Foods, 9, no. 2 (2020),
https://doi.org/10.3390/foods9020138 . .

TY  - JOUR
AU  - Radović, Mirjana
AU  - Milatović, Dragan
AU  - Tešić, Živoslav
AU  - Tosti, Tomislav
AU  - Gašić, Uroš M.
AU  - Dojčinović, Biljana
AU  - Dabić-Zagorac, Dragana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5247
AB  - This paper presents the chemical composition of the fruits of three European plum cultivars ('Cacanska Rana', 'Cacanska Lepotica', and 'Cacanska Najbolja') grafted on four rootstocks (Myrobalan, 'Pixy', Fereley', and 'St Julien A'). The rootstock/cultivar combinations significantly influenced the content of most of the phenolic compounds in the plum skin and flesh. The total anthocyanin content in the skin ranged from 1.87 to 7.33 mg cyanidin-3-glucoside g(-1) fresh weight. The major phenolic compound in the skin was rutin, which ranged from 95 to 238 mg kg(-1), while in the flesh, cinnamic acid and catechin were dominant. The total sugar content in the fruit ranged from 66.2-147 mg g(-1). The highest glucose and fructose contents were in `Cacanska Lepotica', then in `Cacanska Najbolja', and the lowest were in `Cacanska Rana' cultivar. The highest contents of glucose and sucrose were found in fruits from trees grafted on Myrobalan rootstock, and the lowest in fruits from trees grafted on 'St Julien A' rootstock. The rootstocks significantly influenced the potassium, calcium, and copper contents in the plum fruit. The results of this study demonstrate the significant effect of rootstocks on the phenolic and sugar profiles, and on the mineral content of the plum fruits.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Influence of rootstocks on the chemical composition of the fruits of plum cultivars
VL  - 92
DO  - 10.1016/j.jfca.2020.103480
ER  - 

@article{
author = "Radović, Mirjana and Milatović, Dragan and Tešić, Živoslav and Tosti, Tomislav and Gašić, Uroš M. and Dojčinović, Biljana and Dabić-Zagorac, Dragana",
year = "2020",
abstract = "This paper presents the chemical composition of the fruits of three European plum cultivars ('Cacanska Rana', 'Cacanska Lepotica', and 'Cacanska Najbolja') grafted on four rootstocks (Myrobalan, 'Pixy', Fereley', and 'St Julien A'). The rootstock/cultivar combinations significantly influenced the content of most of the phenolic compounds in the plum skin and flesh. The total anthocyanin content in the skin ranged from 1.87 to 7.33 mg cyanidin-3-glucoside g(-1) fresh weight. The major phenolic compound in the skin was rutin, which ranged from 95 to 238 mg kg(-1), while in the flesh, cinnamic acid and catechin were dominant. The total sugar content in the fruit ranged from 66.2-147 mg g(-1). The highest glucose and fructose contents were in `Cacanska Lepotica', then in `Cacanska Najbolja', and the lowest were in `Cacanska Rana' cultivar. The highest contents of glucose and sucrose were found in fruits from trees grafted on Myrobalan rootstock, and the lowest in fruits from trees grafted on 'St Julien A' rootstock. The rootstocks significantly influenced the potassium, calcium, and copper contents in the plum fruit. The results of this study demonstrate the significant effect of rootstocks on the phenolic and sugar profiles, and on the mineral content of the plum fruits.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Influence of rootstocks on the chemical composition of the fruits of plum cultivars",
volume = "92",
doi = "10.1016/j.jfca.2020.103480"
}

Radović, M., Milatović, D., Tešić, Ž., Tosti, T., Gašić, U. M., Dojčinović, B.,& Dabić-Zagorac, D.. (2020). Influence of rootstocks on the chemical composition of the fruits of plum cultivars. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 92.
https://doi.org/10.1016/j.jfca.2020.103480

Radović M, Milatović D, Tešić Ž, Tosti T, Gašić UM, Dojčinović B, Dabić-Zagorac D. Influence of rootstocks on the chemical composition of the fruits of plum cultivars. in Journal of Food Composition and Analysis. 2020;92.
doi:10.1016/j.jfca.2020.103480 .

Radović, Mirjana, Milatović, Dragan, Tešić, Živoslav, Tosti, Tomislav, Gašić, Uroš M., Dojčinović, Biljana, Dabić-Zagorac, Dragana, "Influence of rootstocks on the chemical composition of the fruits of plum cultivars" in Journal of Food Composition and Analysis, 92 (2020),
https://doi.org/10.1016/j.jfca.2020.103480 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milincić, Danijel D.
AU  - Petrović, Tanja
AU  - Krnjaja, Vesna
AU  - Stanojević, Sladjana
AU  - Barać, Miroljub
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5115
AB  - Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a(w) values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.
PB  - MDPI, BASEL
T2  - Toxins
T1  - Mycotoxins and Mycotoxin Producing Fungi in Pollen: Review
IS  - 2
VL  - 11
DO  - 10.3390/toxins11020064
ER  - 

@article{
author = "Kostić, Aleksandar and Milincić, Danijel D. and Petrović, Tanja and Krnjaja, Vesna and Stanojević, Sladjana and Barać, Miroljub and Tešić, Živoslav and Pešić, Mirjana",
year = "2019",
abstract = "Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and a(w) values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.",
publisher = "MDPI, BASEL",
journal = "Toxins",
title = "Mycotoxins and Mycotoxin Producing Fungi in Pollen: Review",
number = "2",
volume = "11",
doi = "10.3390/toxins11020064"
}

Kostić, A., Milincić, D. D., Petrović, T., Krnjaja, V., Stanojević, S., Barać, M., Tešić, Ž.,& Pešić, M.. (2019). Mycotoxins and Mycotoxin Producing Fungi in Pollen: Review. in Toxins
MDPI, BASEL., 11(2).
https://doi.org/10.3390/toxins11020064

Kostić A, Milincić DD, Petrović T, Krnjaja V, Stanojević S, Barać M, Tešić Ž, Pešić M. Mycotoxins and Mycotoxin Producing Fungi in Pollen: Review. in Toxins. 2019;11(2).
doi:10.3390/toxins11020064 .

Kostić, Aleksandar, Milincić, Danijel D., Petrović, Tanja, Krnjaja, Vesna, Stanojević, Sladjana, Barać, Miroljub, Tešić, Živoslav, Pešić, Mirjana, "Mycotoxins and Mycotoxin Producing Fungi in Pollen: Review" in Toxins, 11, no. 2 (2019),
https://doi.org/10.3390/toxins11020064 . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Gašić, Uroš M.
AU  - Pešić, Mirjana
AU  - Stanojević, Sladjana
AU  - Barać, Miroljub
AU  - Mačukanović-Jocić, Marina
AU  - Avramov, Stevan N.
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5116
AB  - This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above-ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC-Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C-glycosides, luteolin O-glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species
IS  - 3
VL  - 16
DO  - 10.1002/cbdv.201800565
ER  - 

@article{
author = "Kostić, Aleksandar and Gašić, Uroš M. and Pešić, Mirjana and Stanojević, Sladjana and Barać, Miroljub and Mačukanović-Jocić, Marina and Avramov, Stevan N. and Tešić, Živoslav",
year = "2019",
abstract = "This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above-ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC-Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C-glycosides, luteolin O-glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species",
number = "3",
volume = "16",
doi = "10.1002/cbdv.201800565"
}

Kostić, A., Gašić, U. M., Pešić, M., Stanojević, S., Barać, M., Mačukanović-Jocić, M., Avramov, S. N.,& Tešić, Ž.. (2019). Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 16(3).
https://doi.org/10.1002/cbdv.201800565

Kostić A, Gašić UM, Pešić M, Stanojević S, Barać M, Mačukanović-Jocić M, Avramov SN, Tešić Ž. Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species. in Chemistry & Biodiversity. 2019;16(3).
doi:10.1002/cbdv.201800565 .

Kostić, Aleksandar, Gašić, Uroš M., Pešić, Mirjana, Stanojević, Sladjana, Barać, Miroljub, Mačukanović-Jocić, Marina, Avramov, Stevan N., Tešić, Živoslav, "Phytochemical Analysis and Total Antioxidant Capacity of Rhizome, Above-Ground Vegetative Parts and Flower of Three Iris Species" in Chemistry & Biodiversity, 16, no. 3 (2019),
https://doi.org/10.1002/cbdv.201800565 . .

TY  - JOUR
AU  - Milincić, Danijel D.
AU  - Popović, Dušanka A.
AU  - Lević, Steva
AU  - Kostić, Aleksandar
AU  - Tešić, Živoslav
AU  - Nedović, Viktor
AU  - Pešić, Mirjana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5004
AB  - Nanotechnology is an emerging field of science, and nanotechnological concepts have been intensively studied for potential applications in the food industry. Nanoparticles (with dimensions ranging from one to several hundred nanometers) have specific characteristics and better functionality, thanks to their size and other physicochemical properties. Polyphenols are recognized as active compounds that have several putative beneficial properties, including antioxidant, antimicrobial, and anticancer activity. However, the use of polyphenols as functional food ingredients faces numerous challenges, such as their poor stability, solubility, and bioavailability. These difficulties could be solved relatively easily by the application of encapsulation. The objective of this review is to present the most recent accomplishments in the usage of polyphenol-loaded nanoparticles in food science. Nanoparticles loaded with polyphenols and their applications as active ingredients for improving physicochemical and functional properties of food, or as components of active packaging materials, were critically reviewed. Potential adverse effects of polyphenol-loaded nanomaterials are also discussed.
PB  - MDPI, BASEL
T2  - Nanomaterials
T1  - Application of Polyphenol-Loaded Nanoparticles in Food Industry
IS  - 11
VL  - 9
DO  - 10.3390/nano9111629
ER  - 

@article{
author = "Milincić, Danijel D. and Popović, Dušanka A. and Lević, Steva and Kostić, Aleksandar and Tešić, Živoslav and Nedović, Viktor and Pešić, Mirjana",
year = "2019",
abstract = "Nanotechnology is an emerging field of science, and nanotechnological concepts have been intensively studied for potential applications in the food industry. Nanoparticles (with dimensions ranging from one to several hundred nanometers) have specific characteristics and better functionality, thanks to their size and other physicochemical properties. Polyphenols are recognized as active compounds that have several putative beneficial properties, including antioxidant, antimicrobial, and anticancer activity. However, the use of polyphenols as functional food ingredients faces numerous challenges, such as their poor stability, solubility, and bioavailability. These difficulties could be solved relatively easily by the application of encapsulation. The objective of this review is to present the most recent accomplishments in the usage of polyphenol-loaded nanoparticles in food science. Nanoparticles loaded with polyphenols and their applications as active ingredients for improving physicochemical and functional properties of food, or as components of active packaging materials, were critically reviewed. Potential adverse effects of polyphenol-loaded nanomaterials are also discussed.",
publisher = "MDPI, BASEL",
journal = "Nanomaterials",
title = "Application of Polyphenol-Loaded Nanoparticles in Food Industry",
number = "11",
volume = "9",
doi = "10.3390/nano9111629"
}

Milincić, D. D., Popović, D. A., Lević, S., Kostić, A., Tešić, Ž., Nedović, V.,& Pešić, M.. (2019). Application of Polyphenol-Loaded Nanoparticles in Food Industry. in Nanomaterials
MDPI, BASEL., 9(11).
https://doi.org/10.3390/nano9111629

Milincić DD, Popović DA, Lević S, Kostić A, Tešić Ž, Nedović V, Pešić M. Application of Polyphenol-Loaded Nanoparticles in Food Industry. in Nanomaterials. 2019;9(11).
doi:10.3390/nano9111629 .

Milincić, Danijel D., Popović, Dušanka A., Lević, Steva, Kostić, Aleksandar, Tešić, Živoslav, Nedović, Viktor, Pešić, Mirjana, "Application of Polyphenol-Loaded Nanoparticles in Food Industry" in Nanomaterials, 9, no. 11 (2019),
https://doi.org/10.3390/nano9111629 . .

TY  - JOUR
AU  - Pešić, Mirjana
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar
AU  - Stanisavljević, Nemanja S.
AU  - Vukotić, Goran
AU  - Kojić, Milan
AU  - Gašić, Uroš M.
AU  - Barać, Miroljub
AU  - Stanojević, Sladjana
AU  - Popović, Dušanka A.
AU  - Banjac, Nebojša
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5034
AB  - The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?
EP  - 44
SP  - 28
VL  - 284
DO  - 10.1016/j.foodchem.2019.01.107
ER  - 

@article{
author = "Pešić, Mirjana and Milincić, Danijel D. and Kostić, Aleksandar and Stanisavljević, Nemanja S. and Vukotić, Goran and Kojić, Milan and Gašić, Uroš M. and Barać, Miroljub and Stanojević, Sladjana and Popović, Dušanka A. and Banjac, Nebojša and Tešić, Živoslav",
year = "2019",
abstract = "The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?",
pages = "44-28",
volume = "284",
doi = "10.1016/j.foodchem.2019.01.107"
}

Pešić, M., Milincić, D. D., Kostić, A., Stanisavljević, N. S., Vukotić, G., Kojić, M., Gašić, U. M., Barać, M., Stanojević, S., Popović, D. A., Banjac, N.,& Tešić, Ž.. (2019). In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry
Elsevier Sci Ltd, Oxford., 284, 28-44.
https://doi.org/10.1016/j.foodchem.2019.01.107

Pešić M, Milincić DD, Kostić A, Stanisavljević NS, Vukotić G, Kojić M, Gašić UM, Barać M, Stanojević S, Popović DA, Banjac N, Tešić Ž. In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry. 2019;284:28-44.
doi:10.1016/j.foodchem.2019.01.107 .

Pešić, Mirjana, Milincić, Danijel D., Kostić, Aleksandar, Stanisavljević, Nemanja S., Vukotić, Goran, Kojić, Milan, Gašić, Uroš M., Barać, Miroljub, Stanojević, Sladjana, Popović, Dušanka A., Banjac, Nebojša, Tešić, Živoslav, "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?" in Food Chemistry, 284 (2019):28-44,
https://doi.org/10.1016/j.foodchem.2019.01.107 . .