TY  - JOUR
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Menković, Jelena
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6326
AB  - During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.
PB  - APS Publications
T2  - Plant Disease
T1  - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
EP  - 833
IS  - 107
SP  - 826
DO  - 10.1094/PDIS-06-22-1425-RE
ER  - 

@article{
author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2023",
abstract = "During May 2016, severe blight symptoms were observed in several
raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic
raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally,
eight raspberry strains, isolated earlier from two similar outbreaks, were
included in the study. Pathogenicity of the strains was confirmed on
detached raspberry and blackberry shoots by reproducing the symptoms
of natural infection. The strains were Gram-negative, fluorescent on
King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic,
arginine dihydrolase negative, and induced hypersensitivity in tobacco
leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all
strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability
tests suggested that isolated strains belong to Pseudomonas syringae.
The syrB gene associated with syringomycin production was detected
in all strains. DNA fingerprints with REP, ERIC, and BOX primers
generated identical profiles for 29 strains, except for strain KBI 222,
which showed a unique genomic fingerprint. In all, 9 of 10 selected
strains exhibited identical sequences of four housekeeping genes: gyrB,
rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in
strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree
based on a concatenated sequence of all four housekeeping genes,
strains clustered within phylogroup 2 (i.e., genomospecies 1) of the
P. syringae species complex, with pathotype strains of P. syringae pv.
aceris and P. syringae pv. solidagae as their closest relatives. There
was no correlation between genotype and geographic origin, particular
outbreak, host, or cultivar.",
publisher = "APS Publications",
journal = "Plant Disease",
title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia",
pages = "833-826",
number = "107",
doi = "10.1094/PDIS-06-22-1425-RE"
}

Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease
APS Publications.(107), 826-833.
https://doi.org/10.1094/PDIS-06-22-1425-RE

Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in Plant Disease. 2023;(107):826-833.
doi:10.1094/PDIS-06-22-1425-RE .

Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in Plant Disease, no. 107 (2023):826-833,
https://doi.org/10.1094/PDIS-06-22-1425-RE . .

TY  - JOUR
AU  - Erken Meral, Songül
AU  - Bibi, Shaheen
AU  - Díaz Rodríguez, Carlos Andrés
AU  - Menković, Jelena
AU  - Bernal, Adriana J.
AU  - Koebnik, Ralf
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6056
AB  - Here, we report the complete genome sequence of the race 4 strain Xanthomonas campestris pv. campestris SB80, which was isolated from a symptomatic white head cabbage leaf in Samsun Province, Turkey, in 2019. The genome consists of a circular chromosome (5,129,762 bp) with a G1C content of 64.98%, for which 4,159 putative protein-coding genes, 2 rRNA operons, 54 tRNAs, and 86 noncoding RNAs (ncRNAs) were predicted.
PB  - American Society for Microbiology
T2  - Microbiology Resource Announcements
T1  - Complete Genome Sequence of Xanthomonas campestris pv. campestris SB80, a Race 4 Strain Isolated from White Head Cabbage in Turkey
IS  - 3
SP  - 00022-22
VL  - 11
DO  - 10.1128/mra.00022-22
ER  - 

@article{
author = "Erken Meral, Songül and Bibi, Shaheen and Díaz Rodríguez, Carlos Andrés and Menković, Jelena and Bernal, Adriana J. and Koebnik, Ralf",
year = "2022",
abstract = "Here, we report the complete genome sequence of the race 4 strain Xanthomonas campestris pv. campestris SB80, which was isolated from a symptomatic white head cabbage leaf in Samsun Province, Turkey, in 2019. The genome consists of a circular chromosome (5,129,762 bp) with a G1C content of 64.98%, for which 4,159 putative protein-coding genes, 2 rRNA operons, 54 tRNAs, and 86 noncoding RNAs (ncRNAs) were predicted.",
publisher = "American Society for Microbiology",
journal = "Microbiology Resource Announcements",
title = "Complete Genome Sequence of Xanthomonas campestris pv. campestris SB80, a Race 4 Strain Isolated from White Head Cabbage in Turkey",
number = "3",
pages = "00022-22",
volume = "11",
doi = "10.1128/mra.00022-22"
}

Erken Meral, S., Bibi, S., Díaz Rodríguez, C. A., Menković, J., Bernal, A. J.,& Koebnik, R.. (2022). Complete Genome Sequence of Xanthomonas campestris pv. campestris SB80, a Race 4 Strain Isolated from White Head Cabbage in Turkey. in Microbiology Resource Announcements
American Society for Microbiology., 11(3), 00022-22.
https://doi.org/10.1128/mra.00022-22

Erken Meral S, Bibi S, Díaz Rodríguez CA, Menković J, Bernal AJ, Koebnik R. Complete Genome Sequence of Xanthomonas campestris pv. campestris SB80, a Race 4 Strain Isolated from White Head Cabbage in Turkey. in Microbiology Resource Announcements. 2022;11(3):00022-22.
doi:10.1128/mra.00022-22 .

Erken Meral, Songül, Bibi, Shaheen, Díaz Rodríguez, Carlos Andrés, Menković, Jelena, Bernal, Adriana J., Koebnik, Ralf, "Complete Genome Sequence of Xanthomonas campestris pv. campestris SB80, a Race 4 Strain Isolated from White Head Cabbage in Turkey" in Microbiology Resource Announcements, 11, no. 3 (2022):00022-22,
https://doi.org/10.1128/mra.00022-22 . .

TY  - JOUR
AU  - Popović, T.
AU  - Menković, Jelena
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5325
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro
EP  - 561
IS  - 2
SP  - 560
VL  - 104
DO  - 10.1094/PDIS-07-19-1422-PDN
ER  - 

@article{
author = "Popović, T. and Menković, Jelena and Prokić, Andjelka and Obradović, Aleksa",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro",
pages = "561-560",
number = "2",
volume = "104",
doi = "10.1094/PDIS-07-19-1422-PDN"
}

Popović, T., Menković, J., Prokić, A.,& Obradović, A.. (2020). First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(2), 560-561.
https://doi.org/10.1094/PDIS-07-19-1422-PDN

Popović T, Menković J, Prokić A, Obradović A. First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease. 2020;104(2):560-561.
doi:10.1094/PDIS-07-19-1422-PDN .

Popović, T., Menković, Jelena, Prokić, Andjelka, Obradović, Aleksa, "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro" in Plant Disease, 104, no. 2 (2020):560-561,
https://doi.org/10.1094/PDIS-07-19-1422-PDN . .