TY  - JOUR
AU  - Mihajlović, Ljubiša
AU  - Cincović, Marko
AU  - Nakov, Dimitar
AU  - Stanković, Branislav
AU  - Miočinović, Jelena
AU  - Hristov, Slavča
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6074
AB  - The Total Bacterial Count (TBC) and the Somatic Cells Count (SCC) in the milk are important indicators of its hygiene and quality. Hygienic conditions in barns, milking procedures, udder hygiene before, during and after milking, milking machine hygiene, as well as milk cooling procedures immediately after milking, have direct or indirect influences on milk hygiene indicators. Poor results of milk hygiene quality, when it comes to SCC and TBC, which are often seen in dairy cattle farms in our country, indicate numerous omissions before and during milking. To determine the possibility of improving milk hygiene quality, training of extension service professionals and farmers was conducted, corrective and preventive measures were determined and the achieved state was monitored on 128 dairy farms where SCC and TBC were observed at regular monthly intervals for 6 months. The results showed a continuous statistically very significant improvement in hygienic conditions in barns, milking procedures, udder hygiene before, during and after milking, milking machine hygiene, as well as milk cooling procedures. This has contributed to a statistically very significant improvement in the hygienic quality of milk, both in SCC and TBC indicators, continuously from month to month, with a visible improvement at the end related to the beginning of the study period. At the begining and the end of survey 19.7% and 50.0% of milk samples belong to 1st class of milk quality, respectively, indicating a significant improvement after implementation of corrective measurements.
PB  - Sciendo
T2  - Acta Veterinaria
T1  - Improvement of hygiene practices and milk hygiene due to systematic implementation of preventive and corrective measures
T1  - Unapređenje higijenske prakse i stanja higijene mleka bazirano na sistematskom sprovođenju preventivnih i korektivnih mera
EP  - 86
IS  - 1
SP  - 76
VL  - 72
DO  - 10.2478/acve-2022-0006
ER  - 

@article{
author = "Mihajlović, Ljubiša and Cincović, Marko and Nakov, Dimitar and Stanković, Branislav and Miočinović, Jelena and Hristov, Slavča",
year = "2022",
abstract = "The Total Bacterial Count (TBC) and the Somatic Cells Count (SCC) in the milk are important indicators of its hygiene and quality. Hygienic conditions in barns, milking procedures, udder hygiene before, during and after milking, milking machine hygiene, as well as milk cooling procedures immediately after milking, have direct or indirect influences on milk hygiene indicators. Poor results of milk hygiene quality, when it comes to SCC and TBC, which are often seen in dairy cattle farms in our country, indicate numerous omissions before and during milking. To determine the possibility of improving milk hygiene quality, training of extension service professionals and farmers was conducted, corrective and preventive measures were determined and the achieved state was monitored on 128 dairy farms where SCC and TBC were observed at regular monthly intervals for 6 months. The results showed a continuous statistically very significant improvement in hygienic conditions in barns, milking procedures, udder hygiene before, during and after milking, milking machine hygiene, as well as milk cooling procedures. This has contributed to a statistically very significant improvement in the hygienic quality of milk, both in SCC and TBC indicators, continuously from month to month, with a visible improvement at the end related to the beginning of the study period. At the begining and the end of survey 19.7% and 50.0% of milk samples belong to 1st class of milk quality, respectively, indicating a significant improvement after implementation of corrective measurements.",
publisher = "Sciendo",
journal = "Acta Veterinaria",
title = "Improvement of hygiene practices and milk hygiene due to systematic implementation of preventive and corrective measures, Unapređenje higijenske prakse i stanja higijene mleka bazirano na sistematskom sprovođenju preventivnih i korektivnih mera",
pages = "86-76",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0006"
}

Mihajlović, L., Cincović, M., Nakov, D., Stanković, B., Miočinović, J.,& Hristov, S.. (2022). Improvement of hygiene practices and milk hygiene due to systematic implementation of preventive and corrective measures. in Acta Veterinaria
Sciendo., 72(1), 76-86.
https://doi.org/10.2478/acve-2022-0006

Mihajlović L, Cincović M, Nakov D, Stanković B, Miočinović J, Hristov S. Improvement of hygiene practices and milk hygiene due to systematic implementation of preventive and corrective measures. in Acta Veterinaria. 2022;72(1):76-86.
doi:10.2478/acve-2022-0006 .

Mihajlović, Ljubiša, Cincović, Marko, Nakov, Dimitar, Stanković, Branislav, Miočinović, Jelena, Hristov, Slavča, "Improvement of hygiene practices and milk hygiene due to systematic implementation of preventive and corrective measures" in Acta Veterinaria, 72, no. 1 (2022):76-86,
https://doi.org/10.2478/acve-2022-0006 . .

TY  - JOUR
AU  - Nakov, Dimitar
AU  - Trajchev, Metodija
AU  - Hristov, Slavča
AU  - Stanković, Branislav
AU  - Cincović, Marko
AU  - Zlatanović, Zvonko
AU  - Bojkovski, Jovan
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6033
AB  - Male piglets are castrated primarily to avoid the unpleasant boar taint in meat, and additionally for the predisposition of castrates to accumulate fat and for their lower risk of developing unwanted behaviours. There are two main strategies available for withdrawing from surgical castration: one is immunocastration and the other is to raise entire male pigs or boars. Additionally, raising intact boars is more profitable because of the production of carcasses with lean meat and better feed conversion. Boars (compared to castrates) exhibit more aggressive, sexual, damaging social behaviour and reduced feeding behaviour with a lower prevalence of sickness behaviour as a result of good health and low susceptibility to chronic inflammation. In this review, the behaviours specific for boars as a result of sexual maturity are reviewed, with an overview of differences in the behaviour of surgically castrated barrows, immunocastrates and boars reared in group-housed systems. The raising of boars allows for good welfare of these animals in early life, but later, on reaching sexual maturity, the welfare of boars can be diminished because of their propensity to aggression and more mounting behaviour than castrates. Innovations in the breeding and management of boars are needed to improve their performance and to reduce welfare implications of these animals raised in social groups, and in particular to minimize deviant behaviours towards pen mates.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Veterinarski Glasnik
T1  - Sexual maturity as risk for development of deviant behaviours in pig production systems with entire malesSexual maturity as risk for development of deviant behaviours in pig production systems with entire males
EP  - 131
IS  - 2
SP  - 112
VL  - 75
DO  - 10.2298/VETGL210727012N
ER  - 

@article{
author = "Nakov, Dimitar and Trajchev, Metodija and Hristov, Slavča and Stanković, Branislav and Cincović, Marko and Zlatanović, Zvonko and Bojkovski, Jovan",
year = "2022",
abstract = "Male piglets are castrated primarily to avoid the unpleasant boar taint in meat, and additionally for the predisposition of castrates to accumulate fat and for their lower risk of developing unwanted behaviours. There are two main strategies available for withdrawing from surgical castration: one is immunocastration and the other is to raise entire male pigs or boars. Additionally, raising intact boars is more profitable because of the production of carcasses with lean meat and better feed conversion. Boars (compared to castrates) exhibit more aggressive, sexual, damaging social behaviour and reduced feeding behaviour with a lower prevalence of sickness behaviour as a result of good health and low susceptibility to chronic inflammation. In this review, the behaviours specific for boars as a result of sexual maturity are reviewed, with an overview of differences in the behaviour of surgically castrated barrows, immunocastrates and boars reared in group-housed systems. The raising of boars allows for good welfare of these animals in early life, but later, on reaching sexual maturity, the welfare of boars can be diminished because of their propensity to aggression and more mounting behaviour than castrates. Innovations in the breeding and management of boars are needed to improve their performance and to reduce welfare implications of these animals raised in social groups, and in particular to minimize deviant behaviours towards pen mates.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Veterinarski Glasnik",
title = "Sexual maturity as risk for development of deviant behaviours in pig production systems with entire malesSexual maturity as risk for development of deviant behaviours in pig production systems with entire males",
pages = "131-112",
number = "2",
volume = "75",
doi = "10.2298/VETGL210727012N"
}

Nakov, D., Trajchev, M., Hristov, S., Stanković, B., Cincović, M., Zlatanović, Z.,& Bojkovski, J.. (2022). Sexual maturity as risk for development of deviant behaviours in pig production systems with entire malesSexual maturity as risk for development of deviant behaviours in pig production systems with entire males. in Veterinarski Glasnik
University of Belgrade, Faculty of Veterinary Medicine., 75(2), 112-131.
https://doi.org/10.2298/VETGL210727012N

Nakov D, Trajchev M, Hristov S, Stanković B, Cincović M, Zlatanović Z, Bojkovski J. Sexual maturity as risk for development of deviant behaviours in pig production systems with entire malesSexual maturity as risk for development of deviant behaviours in pig production systems with entire males. in Veterinarski Glasnik. 2022;75(2):112-131.
doi:10.2298/VETGL210727012N .

Nakov, Dimitar, Trajchev, Metodija, Hristov, Slavča, Stanković, Branislav, Cincović, Marko, Zlatanović, Zvonko, Bojkovski, Jovan, "Sexual maturity as risk for development of deviant behaviours in pig production systems with entire malesSexual maturity as risk for development of deviant behaviours in pig production systems with entire males" in Veterinarski Glasnik, 75, no. 2 (2022):112-131,
https://doi.org/10.2298/VETGL210727012N . .

TY  - JOUR
AU  - Milošević-Stanković, I.
AU  - Hristov, Slavča
AU  - Maksimović, Nevena
AU  - Popović, Blaženka
AU  - Davidović, Vesna
AU  - Mekić, Cvijan
AU  - Dimitrijević, B.
AU  - Cincović, Marko R.
AU  - Stanković, Branislav
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5444
AB  - The investigation was performed on two groups of primiparous and multiparous healthy dewormed Alpine dairy goats (25 each) during peripartal period. Blood samples were collected (jugular venipuncture) 10-15 days before and 10-15 and 30 days after the parturition into BD SST-II Advance (3.5 mL) and BD NaF 3.0 mg Na2EDTA 6.0 mg (2 mL) vacutainers, cooled and centrifuged (1500 r/min, 15 minutes and ≤1300 r/min, 10 minutes, respectively). Glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations in blood sera were determined using A15 automatic spectrophotometric analyzer (Biosystem, Spain). Simultaneously, body condition scoring (BCS) was performed by Villaquiran et al. (2007) method. The obtained data were analyzed by IBM SPSS statistics 21. The glucose concentration inclined to increase in both groups. Differences between glucose levels were significant (P<0.05) 15 days before and 15 days after, as well as 15 and 30 days after the parturition, and very significant (P<0.01) 15 days before and 30 days after the parturition. The BHBA blood levels significantly differed 15 days before and 30 days after and 15 and 30 days after the parturition (P<0.05). BHBA concentration peaked at week 2 postpartum, following the increase of NEFA, providing the substrate for BHBA synthesis. NEFA levels significantly (P<0.05) differed 15 days before and 15 days after the parturition. Goats’ BCS ranged from 2 to 4 and significantly depended on glucose (r=0.392; P<0.05) and BHBA (r=0.317; P<0.05) level 15 days before parturition. BCS 30 days postpartum very significantly depended on the glucose level (r=0.450; P<0.01), significantly higher than the concentration of BHBA (r=0.351; P<0.05) and NEFA concentration (r=-0.304; P<0.05). BCS 15 days before parturition did not depend on the NEFA concentration. Fifteen days after the parturition BCS did not statistically depend on the observed indicators. Obtained data suggest that knowledge of BCS and energy indicators levels may be very useful in research and practice in order to appreciate energy metabolism of pregnant and lactating dairy ruminants, particularly dairy goats. These data are poorly documented for goats, but they can reveal early pathological metabolic changes in transiting female goat organism, enabling successful prophylactic, as well as, therapeutic intervention.
PB  - Massimo Morgante
T2  - Large Animal Review
T1  - Energy metabolism indicators and body condition in peripartal period of alpine goats
EP  - 18
IS  - 1
SP  - 13
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5444
ER  - 

@article{
author = "Milošević-Stanković, I. and Hristov, Slavča and Maksimović, Nevena and Popović, Blaženka and Davidović, Vesna and Mekić, Cvijan and Dimitrijević, B. and Cincović, Marko R. and Stanković, Branislav",
year = "2020",
abstract = "The investigation was performed on two groups of primiparous and multiparous healthy dewormed Alpine dairy goats (25 each) during peripartal period. Blood samples were collected (jugular venipuncture) 10-15 days before and 10-15 and 30 days after the parturition into BD SST-II Advance (3.5 mL) and BD NaF 3.0 mg Na2EDTA 6.0 mg (2 mL) vacutainers, cooled and centrifuged (1500 r/min, 15 minutes and ≤1300 r/min, 10 minutes, respectively). Glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations in blood sera were determined using A15 automatic spectrophotometric analyzer (Biosystem, Spain). Simultaneously, body condition scoring (BCS) was performed by Villaquiran et al. (2007) method. The obtained data were analyzed by IBM SPSS statistics 21. The glucose concentration inclined to increase in both groups. Differences between glucose levels were significant (P<0.05) 15 days before and 15 days after, as well as 15 and 30 days after the parturition, and very significant (P<0.01) 15 days before and 30 days after the parturition. The BHBA blood levels significantly differed 15 days before and 30 days after and 15 and 30 days after the parturition (P<0.05). BHBA concentration peaked at week 2 postpartum, following the increase of NEFA, providing the substrate for BHBA synthesis. NEFA levels significantly (P<0.05) differed 15 days before and 15 days after the parturition. Goats’ BCS ranged from 2 to 4 and significantly depended on glucose (r=0.392; P<0.05) and BHBA (r=0.317; P<0.05) level 15 days before parturition. BCS 30 days postpartum very significantly depended on the glucose level (r=0.450; P<0.01), significantly higher than the concentration of BHBA (r=0.351; P<0.05) and NEFA concentration (r=-0.304; P<0.05). BCS 15 days before parturition did not depend on the NEFA concentration. Fifteen days after the parturition BCS did not statistically depend on the observed indicators. Obtained data suggest that knowledge of BCS and energy indicators levels may be very useful in research and practice in order to appreciate energy metabolism of pregnant and lactating dairy ruminants, particularly dairy goats. These data are poorly documented for goats, but they can reveal early pathological metabolic changes in transiting female goat organism, enabling successful prophylactic, as well as, therapeutic intervention.",
publisher = "Massimo Morgante",
journal = "Large Animal Review",
title = "Energy metabolism indicators and body condition in peripartal period of alpine goats",
pages = "18-13",
number = "1",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5444"
}

Milošević-Stanković, I., Hristov, S., Maksimović, N., Popović, B., Davidović, V., Mekić, C., Dimitrijević, B., Cincović, M. R.,& Stanković, B.. (2020). Energy metabolism indicators and body condition in peripartal period of alpine goats. in Large Animal Review
Massimo Morgante., 26(1), 13-18.
https://hdl.handle.net/21.15107/rcub_agrospace_5444

Milošević-Stanković I, Hristov S, Maksimović N, Popović B, Davidović V, Mekić C, Dimitrijević B, Cincović MR, Stanković B. Energy metabolism indicators and body condition in peripartal period of alpine goats. in Large Animal Review. 2020;26(1):13-18.
https://hdl.handle.net/21.15107/rcub_agrospace_5444 .

Milošević-Stanković, I., Hristov, Slavča, Maksimović, Nevena, Popović, Blaženka, Davidović, Vesna, Mekić, Cvijan, Dimitrijević, B., Cincović, Marko R., Stanković, Branislav, "Energy metabolism indicators and body condition in peripartal period of alpine goats" in Large Animal Review, 26, no. 1 (2020):13-18,
https://hdl.handle.net/21.15107/rcub_agrospace_5444 .

TY  - JOUR
AU  - Majkić, Mira
AU  - Cincović, Marko R.
AU  - Belić, Branislava
AU  - Plavsa, Nada
AU  - Hristov, Slavča
AU  - Stanković, Branislav
AU  - Popović-Vranješ, Anka
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5128
AB  - The temperature-humidity index (THI) is conventionally used to measure heat stress. The aim of this paper is to determine the influence of THI and THI+metabolic parameters in prediction of milk production. A total of thirty Holstein-Friesian cows were exposed to heat stress and the THI values, milk production and metabolite concentrations were measured on days 0 (in the thermoneutral period), 7 and 14 after the exposure. The average daily THI values obtained were 65 +/- 1.05 (day 0), 75 +/- 1.1 (day 7) and 77 +/- 1.4 (day 14). Heat-stressed cows were found to exhibit a decrease in milk production, contents of glucose and non-esterified fatty acids (NEFA) and the glucose-to-insulin ratio (G:I), whereas the levels of insulin, tumor necrosis factor-alpha (TNF-alpha) and the revised quantitative insulin sensitivity check index (RQUICKI) turned out to be elevated. THI can explain 37% of the milk production variance. The percent variance explained is significantly higher after the addition of metabolic parameters in the THI+glucose (58%) and THI+glucose+TNF-alpha (65%) models and non-significantly higher after the addition of other metabolic parameters. Partial correlation analysis showed that a correlation between milk production and THI is significantly dependent on glucose. TNF-alpha showed a tendency to regulate the above-mentioned correlation, while other metabolic parameters showed a non-significant effect on the correlation between THI and milk production. It was concluded that variation in milk production during heat stress could be better predicted when THI is used in combination with glucose and TNF-alpha as a metabolic predictor. The effect of glucose and TNF-alpha in milk production during heat stress would be investigated in the next stage of our research.
PB  - Polish Academy of Sciences
T2  - Animal Science Papers and Reports
T1  - Variations in milk production based on the temperature-humidity index and blood metabolic parameters in cows during exposure to heat stress
EP  - 369
IS  - 4
SP  - 359
VL  - 36
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5128
ER  - 

@article{
author = "Majkić, Mira and Cincović, Marko R. and Belić, Branislava and Plavsa, Nada and Hristov, Slavča and Stanković, Branislav and Popović-Vranješ, Anka",
year = "2019",
abstract = "The temperature-humidity index (THI) is conventionally used to measure heat stress. The aim of this paper is to determine the influence of THI and THI+metabolic parameters in prediction of milk production. A total of thirty Holstein-Friesian cows were exposed to heat stress and the THI values, milk production and metabolite concentrations were measured on days 0 (in the thermoneutral period), 7 and 14 after the exposure. The average daily THI values obtained were 65 +/- 1.05 (day 0), 75 +/- 1.1 (day 7) and 77 +/- 1.4 (day 14). Heat-stressed cows were found to exhibit a decrease in milk production, contents of glucose and non-esterified fatty acids (NEFA) and the glucose-to-insulin ratio (G:I), whereas the levels of insulin, tumor necrosis factor-alpha (TNF-alpha) and the revised quantitative insulin sensitivity check index (RQUICKI) turned out to be elevated. THI can explain 37% of the milk production variance. The percent variance explained is significantly higher after the addition of metabolic parameters in the THI+glucose (58%) and THI+glucose+TNF-alpha (65%) models and non-significantly higher after the addition of other metabolic parameters. Partial correlation analysis showed that a correlation between milk production and THI is significantly dependent on glucose. TNF-alpha showed a tendency to regulate the above-mentioned correlation, while other metabolic parameters showed a non-significant effect on the correlation between THI and milk production. It was concluded that variation in milk production during heat stress could be better predicted when THI is used in combination with glucose and TNF-alpha as a metabolic predictor. The effect of glucose and TNF-alpha in milk production during heat stress would be investigated in the next stage of our research.",
publisher = "Polish Academy of Sciences",
journal = "Animal Science Papers and Reports",
title = "Variations in milk production based on the temperature-humidity index and blood metabolic parameters in cows during exposure to heat stress",
pages = "369-359",
number = "4",
volume = "36",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5128"
}

Majkić, M., Cincović, M. R., Belić, B., Plavsa, N., Hristov, S., Stanković, B.,& Popović-Vranješ, A.. (2019). Variations in milk production based on the temperature-humidity index and blood metabolic parameters in cows during exposure to heat stress. in Animal Science Papers and Reports
Polish Academy of Sciences., 36(4), 359-369.
https://hdl.handle.net/21.15107/rcub_agrospace_5128

Majkić M, Cincović MR, Belić B, Plavsa N, Hristov S, Stanković B, Popović-Vranješ A. Variations in milk production based on the temperature-humidity index and blood metabolic parameters in cows during exposure to heat stress. in Animal Science Papers and Reports. 2019;36(4):359-369.
https://hdl.handle.net/21.15107/rcub_agrospace_5128 .

Majkić, Mira, Cincović, Marko R., Belić, Branislava, Plavsa, Nada, Hristov, Slavča, Stanković, Branislav, Popović-Vranješ, Anka, "Variations in milk production based on the temperature-humidity index and blood metabolic parameters in cows during exposure to heat stress" in Animal Science Papers and Reports, 36, no. 4 (2019):359-369,
https://hdl.handle.net/21.15107/rcub_agrospace_5128 .

TY  - JOUR
AU  - Cincović, Marko R.
AU  - Belić, Branislava
AU  - Radojičić, Biljana
AU  - Hristov, Slavča
AU  - Djoković, Radojica
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2892
AB  - The aim of this study was to examine the characteristics of the metabolic profile and complete blood count in cows in the periparturient period on the basis of the intensity of lipolysis and ketogenesis (concentration of non esterified fatty acid - NEFA and betahydroxybutyrate - BHB). Based on median values of NEFA and BHB cows were divided into 3 groups: cows physiologically burdened with catabolism (NEFA and BHB levels above the median one week after parturition), cows significantly burdened with catabolism (NEFA and BHB levels above the median one week before and after parturition) and cows that are not burdened with catabolism (NEFA and BHB below the median, i.e. the control group). The median value of NEFA was 0.27 mmol/L one week before parturition and 0.61 mmol/L one week after it. The median value of BHB was 0.51 mmol/L one week before parturition and 0.99 mmol/L one week after it. A significant group effect was shown for each week separately, so that cows physiologically burdened with catabolism and/or cows significantly burdened with catabolism compared to the control group have the following features of the metabolic profile and complete blood count: higher concentrations of NEFA and BHB (weeks: -1, 1, 2, 4, 8), lower concentrations of glucose (weeks: 1, 4), lower concentration of cholesterol (week 8), lower concentrations of total protein (weeks: 1, 2), lower concentrations of urea (weeks: 1, 2, 4, 8) and a higher concentration of bilirubin (weeks: - 1, 1, 2, 4, 8), increased levels of AST (weeks: -1, 1) and ALT (weeks: -1, 2), lower value of Ca (week -1), lower hemoglobin concentration (week -1), lower white blood cell count (week 4), a larger number of neutrophils (weeks: -1, 1, 2) and a higher number of lymphocytes (week 4). Using the method of factor analysis and principal components showed that NEFA, BHB and glucose are the major components that affect the metabolic profile and blood count, making 71.8% of the variability of all parameters. Cows with hypoglycemia, hypocalcemia, hypoalbuminemia, hyperbilirubinemia, decreased hemoglobin concentration and/or red blood cell count and neutrophil to lymphocyte ratio >1 showed significantly higher concentrations of NEFA and BHB compared to cows with parameters within normal ranges. Complete ROC (receiver operating characteristics) analysis showed that NEFA and BHB are important diagnostic indicators that allow the separation of cows with parameters out of the reference values from cows with normal values of parameters in the metabolic profile and blood count (0.5 lt AUC lt 0.87). Reliability of identification of cows with parameters that are out of the reference value increases with increasing concentrations of NEFA and BHB, which confirms that intense lipolysis and ketogenesis start a series of compensatory processes in the metabolism in cows. The results confirm that NEFA and BHB significantly affect the value of numerous metabolic and hematological parameters in the periparturient period.
AB  - Cilj ovog rada je bio da se ispitaju karakteristike metaboličkog profila i krvne slike krava u peripartalnom periodu na osnovu intenziteta lipolize i ketogeneze (koncentracija NEFA i BHB). Na osnovu vrednosti medijane NEFA i BHB krave su podeljene u 3 grupe: krave fiziološki opterećene katabolizmom (NEFA i BHB iznad medijane u nedelji posle partusa), krave značajno opterećene katabolizmom (NEFA i BHB iznad medijane u nedelji pre i posle partusa) i krave koje nisu opterećene katabolizmom (NEFA i BHB ispod medijane). Srednja vrednost NEFA bila je 0.27 mmol/l u nedelji pre partusa i 0.61 mmol/l u nedelji posle partusa. Srednja vrednost BHB iznosila je 0.51mmol/l u nedelji pre partusa i 0.99 mmol/l u nedelji posle partusa. Dokazan je signifikantan uticaj grupe za svaku nedelju posebno, tako da krave fiziološki opterećene katabolizmom i/ili značajno opterećene katabolizmom u odnosu na kontrolnu grupu imaju: višu koncentraciju NEFA i BHB (nedelje: -1, 1, 2, 4, 8), nižu koncentraciju glukoze (nedelje:1, 4), nižu koncentraciju holesterola (nedelja 8), nižu koncentraciju ukupnih proteina (nedelje:1, 2), nižu koncentraciju uree (nedelje:1, 2, 4, 8) i višu koncentraciju bilirubina (nedelje: -1, 1, 2, 4, 8), višu aktivnost AST (nedelje: -1, 1) i ALT (nedelje: -1, 2), nižu vrednost Ca (nedelja -1), nižu koncentraciju hemoglobina (nedelja -1), manji broj leukocita (nedelja 4), veći broj neutrofila (nedelje: -1, 1, 2) i veći broj limfocita (nedelja 4). Faktorskom analizom i metodom glavnih komponenti utvrđeno je su NEFA, BHB i glukoza najznačajnije komponente koje utiču na metabolički profil i krvnu sliku čineći 71.8% varijabilnosti svih parametara. Krave kod kojih je utvrđena hipoglikemija, hipokalcemija, hipoalbuminemija, hiperbilirubinemija, snižena koncentracija hemoglobina i/ili broj eritrocita i odnos neutrofila i limfocita >1 imaju značajno višu koncentraciju NEFA i BHB u odnosu na krave sa vrednostima ovih parametara u okviru referentnih. Kompletnom ROC (receiver operating characteristics) analizom je dokazano da su NEFA i BHB značajni dijagnostički indikatori koji omogućavaju razdvajanje krava sa parametrima izvan referentnih vrednosti od krava čiji su parametri u okviru referentnih vrednosti (0.5 lt AUC lt  0.87). Pouzdanost identifikacije krava sa parametrima izvan referentneih vrednosti raste sa porastom koncentracije NEFA i BHB (intenzivniji katabolizam), što potvrđuje da intenzivna lipoliza i ketogeneza pokreću niz kompenzatornih procesa u metabolizmu krava. Dobijeni rezultati potvrđuju da NEFA i BHB značajno utiču na vrednost metaboličkih i hematoloških parametara u peripartalnom periodu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Influence of lipolysis and ketogenesis to metabolic and hematological parameters in dairy cows during periparturient period
T1  - Uticaj lipolize i ketogeneze na metaboličke i hematološke parametre kod mlečnih krava tokom peripartalnog perioda
EP  - 444
IS  - 4
SP  - 429
VL  - 62
DO  - 10.2298/AVB1204429C
ER  - 

@article{
author = "Cincović, Marko R. and Belić, Branislava and Radojičić, Biljana and Hristov, Slavča and Djoković, Radojica",
year = "2012",
abstract = "The aim of this study was to examine the characteristics of the metabolic profile and complete blood count in cows in the periparturient period on the basis of the intensity of lipolysis and ketogenesis (concentration of non esterified fatty acid - NEFA and betahydroxybutyrate - BHB). Based on median values of NEFA and BHB cows were divided into 3 groups: cows physiologically burdened with catabolism (NEFA and BHB levels above the median one week after parturition), cows significantly burdened with catabolism (NEFA and BHB levels above the median one week before and after parturition) and cows that are not burdened with catabolism (NEFA and BHB below the median, i.e. the control group). The median value of NEFA was 0.27 mmol/L one week before parturition and 0.61 mmol/L one week after it. The median value of BHB was 0.51 mmol/L one week before parturition and 0.99 mmol/L one week after it. A significant group effect was shown for each week separately, so that cows physiologically burdened with catabolism and/or cows significantly burdened with catabolism compared to the control group have the following features of the metabolic profile and complete blood count: higher concentrations of NEFA and BHB (weeks: -1, 1, 2, 4, 8), lower concentrations of glucose (weeks: 1, 4), lower concentration of cholesterol (week 8), lower concentrations of total protein (weeks: 1, 2), lower concentrations of urea (weeks: 1, 2, 4, 8) and a higher concentration of bilirubin (weeks: - 1, 1, 2, 4, 8), increased levels of AST (weeks: -1, 1) and ALT (weeks: -1, 2), lower value of Ca (week -1), lower hemoglobin concentration (week -1), lower white blood cell count (week 4), a larger number of neutrophils (weeks: -1, 1, 2) and a higher number of lymphocytes (week 4). Using the method of factor analysis and principal components showed that NEFA, BHB and glucose are the major components that affect the metabolic profile and blood count, making 71.8% of the variability of all parameters. Cows with hypoglycemia, hypocalcemia, hypoalbuminemia, hyperbilirubinemia, decreased hemoglobin concentration and/or red blood cell count and neutrophil to lymphocyte ratio >1 showed significantly higher concentrations of NEFA and BHB compared to cows with parameters within normal ranges. Complete ROC (receiver operating characteristics) analysis showed that NEFA and BHB are important diagnostic indicators that allow the separation of cows with parameters out of the reference values from cows with normal values of parameters in the metabolic profile and blood count (0.5 lt AUC lt 0.87). Reliability of identification of cows with parameters that are out of the reference value increases with increasing concentrations of NEFA and BHB, which confirms that intense lipolysis and ketogenesis start a series of compensatory processes in the metabolism in cows. The results confirm that NEFA and BHB significantly affect the value of numerous metabolic and hematological parameters in the periparturient period., Cilj ovog rada je bio da se ispitaju karakteristike metaboličkog profila i krvne slike krava u peripartalnom periodu na osnovu intenziteta lipolize i ketogeneze (koncentracija NEFA i BHB). Na osnovu vrednosti medijane NEFA i BHB krave su podeljene u 3 grupe: krave fiziološki opterećene katabolizmom (NEFA i BHB iznad medijane u nedelji posle partusa), krave značajno opterećene katabolizmom (NEFA i BHB iznad medijane u nedelji pre i posle partusa) i krave koje nisu opterećene katabolizmom (NEFA i BHB ispod medijane). Srednja vrednost NEFA bila je 0.27 mmol/l u nedelji pre partusa i 0.61 mmol/l u nedelji posle partusa. Srednja vrednost BHB iznosila je 0.51mmol/l u nedelji pre partusa i 0.99 mmol/l u nedelji posle partusa. Dokazan je signifikantan uticaj grupe za svaku nedelju posebno, tako da krave fiziološki opterećene katabolizmom i/ili značajno opterećene katabolizmom u odnosu na kontrolnu grupu imaju: višu koncentraciju NEFA i BHB (nedelje: -1, 1, 2, 4, 8), nižu koncentraciju glukoze (nedelje:1, 4), nižu koncentraciju holesterola (nedelja 8), nižu koncentraciju ukupnih proteina (nedelje:1, 2), nižu koncentraciju uree (nedelje:1, 2, 4, 8) i višu koncentraciju bilirubina (nedelje: -1, 1, 2, 4, 8), višu aktivnost AST (nedelje: -1, 1) i ALT (nedelje: -1, 2), nižu vrednost Ca (nedelja -1), nižu koncentraciju hemoglobina (nedelja -1), manji broj leukocita (nedelja 4), veći broj neutrofila (nedelje: -1, 1, 2) i veći broj limfocita (nedelja 4). Faktorskom analizom i metodom glavnih komponenti utvrđeno je su NEFA, BHB i glukoza najznačajnije komponente koje utiču na metabolički profil i krvnu sliku čineći 71.8% varijabilnosti svih parametara. Krave kod kojih je utvrđena hipoglikemija, hipokalcemija, hipoalbuminemija, hiperbilirubinemija, snižena koncentracija hemoglobina i/ili broj eritrocita i odnos neutrofila i limfocita >1 imaju značajno višu koncentraciju NEFA i BHB u odnosu na krave sa vrednostima ovih parametara u okviru referentnih. Kompletnom ROC (receiver operating characteristics) analizom je dokazano da su NEFA i BHB značajni dijagnostički indikatori koji omogućavaju razdvajanje krava sa parametrima izvan referentnih vrednosti od krava čiji su parametri u okviru referentnih vrednosti (0.5 lt AUC lt  0.87). Pouzdanost identifikacije krava sa parametrima izvan referentneih vrednosti raste sa porastom koncentracije NEFA i BHB (intenzivniji katabolizam), što potvrđuje da intenzivna lipoliza i ketogeneza pokreću niz kompenzatornih procesa u metabolizmu krava. Dobijeni rezultati potvrđuju da NEFA i BHB značajno utiču na vrednost metaboličkih i hematoloških parametara u peripartalnom periodu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Influence of lipolysis and ketogenesis to metabolic and hematological parameters in dairy cows during periparturient period, Uticaj lipolize i ketogeneze na metaboličke i hematološke parametre kod mlečnih krava tokom peripartalnog perioda",
pages = "444-429",
number = "4",
volume = "62",
doi = "10.2298/AVB1204429C"
}

Cincović, M. R., Belić, B., Radojičić, B., Hristov, S.,& Djoković, R.. (2012). Influence of lipolysis and ketogenesis to metabolic and hematological parameters in dairy cows during periparturient period. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 429-444.
https://doi.org/10.2298/AVB1204429C

Cincović MR, Belić B, Radojičić B, Hristov S, Djoković R. Influence of lipolysis and ketogenesis to metabolic and hematological parameters in dairy cows during periparturient period. in Acta veterinaria. 2012;62(4):429-444.
doi:10.2298/AVB1204429C .

Cincović, Marko R., Belić, Branislava, Radojičić, Biljana, Hristov, Slavča, Djoković, Radojica, "Influence of lipolysis and ketogenesis to metabolic and hematological parameters in dairy cows during periparturient period" in Acta veterinaria, 62, no. 4 (2012):429-444,
https://doi.org/10.2298/AVB1204429C . .