TY  - CONF
AU  - Lević, Steva
AU  - Salević, Ana
AU  - Rac, Vladislav
AU  - Nedović, Viktor
AU  - Antić, Mališa
AU  - Rabrenović, Biljana
AU  - Malićanin, Marko
AU  - Rakić, Vesna
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6861
AB  - In recent decades, wine production has been expanding, with emerging wine regions and new small and medium-sized wineries. Winemaking produces large amounts of grape seeds, often discarded as waste, which are rich in oil, dietary fibers, tocopherols, and polyphenols. If used, the seeds are usually utilized as a source of oil, while the cake remaining after oil extraction is the additional waste with limited usage. Thus, we are working to establish the procedures for the complete utilization of grape seeds with zero byproducts. Pinot Noir variety from a small winery, Zmajevac, Serbia, was used. The main benefit of the oil, obtained by cold pressing, is the high content of unsaturated fatty acids, i.e., linoleic (70%) and oleic acid (19%), with a total oil content of 15 % and an 11 % yield after cold pressing. To facilitate handling, potential addition to food products, and its protection, grape seed oil was encapsulated into calcium alginate-based gel capsules. Encapsulation techniques, electrostatic extrusion, and atomization were used to produce finely powdered encapsulates with encapsulation efficiencies of close to 90 %. The potential use of these capsules is in the substitution of saturated fats in food products where the capsules, apart from protecting oil, could prevent its leakage from food matrices. The results showed that the oil cake could be a promising source of dietary fibers. It contained up to 38 % of insoluble dietary fibers and around 2% of soluble ones (w/w). The high content of insoluble dietary fibers is a basis for further modifications to increase the content of soluble fibers and modulate the nutritional properties of the oil cake. Thus, we tested the enzymatic and hydrothermal processes as modification procedures. The results showed that the enzymatic treatment was more promising regarding the control of soluble/insoluble fibers ratio. Also, the enzymatic process should be more cost- effective regarding capacity and energy consumption.
PB  - European Declaration on Food, Technology and Nutrition Network, Ljubljana
C3  - CEFood Congress Book. 11th Central European Congress on Food and Nutrition “Food, technology and nutrition for healthy people in a healthy environment“
T1  - Towards zero waste food production: utilization of grape seeds
SP  - P-144
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6861
ER  - 

@conference{
author = "Lević, Steva and Salević, Ana and Rac, Vladislav and Nedović, Viktor and Antić, Mališa and Rabrenović, Biljana and Malićanin, Marko and Rakić, Vesna",
year = "2022",
abstract = "In recent decades, wine production has been expanding, with emerging wine regions and new small and medium-sized wineries. Winemaking produces large amounts of grape seeds, often discarded as waste, which are rich in oil, dietary fibers, tocopherols, and polyphenols. If used, the seeds are usually utilized as a source of oil, while the cake remaining after oil extraction is the additional waste with limited usage. Thus, we are working to establish the procedures for the complete utilization of grape seeds with zero byproducts. Pinot Noir variety from a small winery, Zmajevac, Serbia, was used. The main benefit of the oil, obtained by cold pressing, is the high content of unsaturated fatty acids, i.e., linoleic (70%) and oleic acid (19%), with a total oil content of 15 % and an 11 % yield after cold pressing. To facilitate handling, potential addition to food products, and its protection, grape seed oil was encapsulated into calcium alginate-based gel capsules. Encapsulation techniques, electrostatic extrusion, and atomization were used to produce finely powdered encapsulates with encapsulation efficiencies of close to 90 %. The potential use of these capsules is in the substitution of saturated fats in food products where the capsules, apart from protecting oil, could prevent its leakage from food matrices. The results showed that the oil cake could be a promising source of dietary fibers. It contained up to 38 % of insoluble dietary fibers and around 2% of soluble ones (w/w). The high content of insoluble dietary fibers is a basis for further modifications to increase the content of soluble fibers and modulate the nutritional properties of the oil cake. Thus, we tested the enzymatic and hydrothermal processes as modification procedures. The results showed that the enzymatic treatment was more promising regarding the control of soluble/insoluble fibers ratio. Also, the enzymatic process should be more cost- effective regarding capacity and energy consumption.",
publisher = "European Declaration on Food, Technology and Nutrition Network, Ljubljana",
journal = "CEFood Congress Book. 11th Central European Congress on Food and Nutrition “Food, technology and nutrition for healthy people in a healthy environment“",
title = "Towards zero waste food production: utilization of grape seeds",
pages = "P-144",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6861"
}

Lević, S., Salević, A., Rac, V., Nedović, V., Antić, M., Rabrenović, B., Malićanin, M.,& Rakić, V.. (2022). Towards zero waste food production: utilization of grape seeds. in CEFood Congress Book. 11th Central European Congress on Food and Nutrition “Food, technology and nutrition for healthy people in a healthy environment“
European Declaration on Food, Technology and Nutrition Network, Ljubljana., P-144.
https://hdl.handle.net/21.15107/rcub_agrospace_6861

Lević S, Salević A, Rac V, Nedović V, Antić M, Rabrenović B, Malićanin M, Rakić V. Towards zero waste food production: utilization of grape seeds. in CEFood Congress Book. 11th Central European Congress on Food and Nutrition “Food, technology and nutrition for healthy people in a healthy environment“. 2022;:P-144.
https://hdl.handle.net/21.15107/rcub_agrospace_6861 .

Lević, Steva, Salević, Ana, Rac, Vladislav, Nedović, Viktor, Antić, Mališa, Rabrenović, Biljana, Malićanin, Marko, Rakić, Vesna, "Towards zero waste food production: utilization of grape seeds" in CEFood Congress Book. 11th Central European Congress on Food and Nutrition “Food, technology and nutrition for healthy people in a healthy environment“ (2022):P-144,
https://hdl.handle.net/21.15107/rcub_agrospace_6861 .

TY  - CONF
AU  - Stojićević, Aleksandra
AU  - Alimpić, Ana
AU  - Pantić, Milena
AU  - Antić, Mališa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6628
AB  - Basil (Ocimum basilicum L.) has a long traditional use as spice in both folk and conventional medicine. Basil extracts (BEs) as well as essential oil (EO) represents a rich source of phytochemicals, many of which possess diverse biological activities. The objectives of this study were to assess antioxidant and antibacterial activities of BEs and EO, obtained from plants cultivated in Republic of Serbia. EO was isolated by hydrodistillation, while BEs were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. In prepared BEs total phenolic (TPC) and total flavonoid content (TFC) were determined spectrophotometrically. Antioxidant activity was evaluated by DPPH and FRAP assays. A higher TPC was obtained using 70% ethanol, while 96% ethanol proved to be more effective for the extraction of flavonoids. In both antioxidant assays, BEs showed remarkable antioxidant properties compared to EO. Also, regardless the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. Both antioxidant assays strongly correlated with TPC than with TFC. For antibacterial activity assay, minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. Typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes). Opposite to results of antioxidant activity, EO expressed superior antibacterial activity. Based on the obtained results, it can be concluded that basil represents powerful source of biologically active components which can express strong antioxidant and antibacterial activity.
C3  - 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland
T1  - Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts
DO  - 10.3390/ECMC2022-13482
ER  - 

@conference{
author = "Stojićević, Aleksandra and Alimpić, Ana and Pantić, Milena and Antić, Mališa",
year = "2022",
abstract = "Basil (Ocimum basilicum L.) has a long traditional use as spice in both folk and conventional medicine. Basil extracts (BEs) as well as essential oil (EO) represents a rich source of phytochemicals, many of which possess diverse biological activities. The objectives of this study were to assess antioxidant and antibacterial activities of BEs and EO, obtained from plants cultivated in Republic of Serbia. EO was isolated by hydrodistillation, while BEs were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. In prepared BEs total phenolic (TPC) and total flavonoid content (TFC) were determined spectrophotometrically. Antioxidant activity was evaluated by DPPH and FRAP assays. A higher TPC was obtained using 70% ethanol, while 96% ethanol proved to be more effective for the extraction of flavonoids. In both antioxidant assays, BEs showed remarkable antioxidant properties compared to EO. Also, regardless the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. Both antioxidant assays strongly correlated with TPC than with TFC. For antibacterial activity assay, minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. Typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes). Opposite to results of antioxidant activity, EO expressed superior antibacterial activity. Based on the obtained results, it can be concluded that basil represents powerful source of biologically active components which can express strong antioxidant and antibacterial activity.",
journal = "8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland",
title = "Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts",
doi = "10.3390/ECMC2022-13482"
}

Stojićević, A., Alimpić, A., Pantić, M.,& Antić, M.. (2022). Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts. in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland.
https://doi.org/10.3390/ECMC2022-13482

Stojićević A, Alimpić A, Pantić M, Antić M. Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts. in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland. 2022;.
doi:10.3390/ECMC2022-13482 .

Stojićević, Aleksandra, Alimpić, Ana, Pantić, Milena, Antić, Mališa, "Basil (Ocimum basilicum L.) cultivated in Serbia: antioxidant and antibacterial activity of essential oil and extracts" in 8th International Electronic Conference on Medicinal Chemistry, Basel, Switzerland (2022),
https://doi.org/10.3390/ECMC2022-13482 . .

TY  - JOUR
AU  - Stojanović, Branislav Đ.
AU  - Stojanović, Zdenka M.
AU  - Marjanović, Sonja S.
AU  - Janković, Saša D.
AU  - Antić, Mališa P.
AU  - Balaban, Milica R.
AU  - Antić, Vesna V.
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6284
AB  - Macroelements such as Na, K, Ca, and Mg play a significant physiological role, and their inadequate intake has been linked to severe diseases, such as high blood pressure. Data on risk assessment for human health in Serbia, from the intake of these macroelements through the consumption of canned food, are minimal. Therefore, the content of Na, K, Ca, and Mg in five types of canned meat that members of the Serbian Armed Forces regularly use was examined. Macroelements were determined by inductively coupled plasma mass spectrometry in cans of beef goulash, pork ragout, spam, liver pate, and meatballs in tomato sauce, which were stored from one month to six years. The sodium content was significantly higher than the potassium content in all types of food, so the Na/K ratio below 1, desirable for good health, was not found in any of the analyzed products. Also, a significant number of samples had an unfavorable Ca/Mg ratio above 1. However, due to the low consumption of canned food by members of the Serbian Armed Forces, its contribution to the average daily intake of macroelements is almost negligible. The concentration of macroelements decreased with the shelf life, while a significant source of Ka and Mg, among analyzed ingredients, was ground red pepper. © 2022 University of Belgrade - Faculty of Agriculture. All Rights Reserved.
T2  - Journal of Agricultural Sciences (Belgrade)
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - CANNED MEAT PRODUCTS FOR MEMBERS OF THE SERBIAN ARMED FORCES: Na, K, Ca, AND Mg CONTENT AND HEALTH RISKS/BENEFITS
EP  - 431
IS  - 4
SP  - 415
VL  - 67
DO  - 10.2298/JAS2204415S
ER  - 

@article{
author = "Stojanović, Branislav Đ. and Stojanović, Zdenka M. and Marjanović, Sonja S. and Janković, Saša D. and Antić, Mališa P. and Balaban, Milica R. and Antić, Vesna V.",
year = "2022",
abstract = "Macroelements such as Na, K, Ca, and Mg play a significant physiological role, and their inadequate intake has been linked to severe diseases, such as high blood pressure. Data on risk assessment for human health in Serbia, from the intake of these macroelements through the consumption of canned food, are minimal. Therefore, the content of Na, K, Ca, and Mg in five types of canned meat that members of the Serbian Armed Forces regularly use was examined. Macroelements were determined by inductively coupled plasma mass spectrometry in cans of beef goulash, pork ragout, spam, liver pate, and meatballs in tomato sauce, which were stored from one month to six years. The sodium content was significantly higher than the potassium content in all types of food, so the Na/K ratio below 1, desirable for good health, was not found in any of the analyzed products. Also, a significant number of samples had an unfavorable Ca/Mg ratio above 1. However, due to the low consumption of canned food by members of the Serbian Armed Forces, its contribution to the average daily intake of macroelements is almost negligible. The concentration of macroelements decreased with the shelf life, while a significant source of Ka and Mg, among analyzed ingredients, was ground red pepper. © 2022 University of Belgrade - Faculty of Agriculture. All Rights Reserved.",
journal = "Journal of Agricultural Sciences (Belgrade), Journal of Agricultural Sciences (Belgrade)",
title = "CANNED MEAT PRODUCTS FOR MEMBERS OF THE SERBIAN ARMED FORCES: Na, K, Ca, AND Mg CONTENT AND HEALTH RISKS/BENEFITS",
pages = "431-415",
number = "4",
volume = "67",
doi = "10.2298/JAS2204415S"
}

Stojanović, B. Đ., Stojanović, Z. M., Marjanović, S. S., Janković, S. D., Antić, M. P., Balaban, M. R.,& Antić, V. V.. (2022). CANNED MEAT PRODUCTS FOR MEMBERS OF THE SERBIAN ARMED FORCES: Na, K, Ca, AND Mg CONTENT AND HEALTH RISKS/BENEFITS. in Journal of Agricultural Sciences (Belgrade), 67(4), 415-431.
https://doi.org/10.2298/JAS2204415S

Stojanović BĐ, Stojanović ZM, Marjanović SS, Janković SD, Antić MP, Balaban MR, Antić VV. CANNED MEAT PRODUCTS FOR MEMBERS OF THE SERBIAN ARMED FORCES: Na, K, Ca, AND Mg CONTENT AND HEALTH RISKS/BENEFITS. in Journal of Agricultural Sciences (Belgrade). 2022;67(4):415-431.
doi:10.2298/JAS2204415S .

Stojanović, Branislav Đ., Stojanović, Zdenka M., Marjanović, Sonja S., Janković, Saša D., Antić, Mališa P., Balaban, Milica R., Antić, Vesna V., "CANNED MEAT PRODUCTS FOR MEMBERS OF THE SERBIAN ARMED FORCES: Na, K, Ca, AND Mg CONTENT AND HEALTH RISKS/BENEFITS" in Journal of Agricultural Sciences (Belgrade), 67, no. 4 (2022):415-431,
https://doi.org/10.2298/JAS2204415S . .

TY  - CONF
AU  - Stojićević, Aleksandra
AU  - Alimpić Aradski, Ana
AU  - Pantić, Milena
AU  - Pantelić, Nebojša
AU  - Rabrenović, Biljana
AU  - Duletić-Laušević, Sonja
AU  - Antić, Mališa
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6630
AB  - Medicinal plants represent a rich source of phytochemicals, many of which possess diverse biological activities, including antioxidants. The objectives of this study were to assess antioxidant, antibacterial and anticancer activities of savory (Satureja montana L.) ethanolic extracts and to monitor the changes of oxidative status during long-term storage conditions of the cold pressed sunflower oil (CPSU) with the addition of extracts at various concentrations.  Extracts were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. Antioxidant activity was evaluated by DPPH and ꞵ-carotene bleaching assays. In both assays, extracts showed remarkable antioxidative properties which were equal or stronger than those of BHT and BHA. Also, regardless of the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. For antibacterial activity assay, minimal inhibitory concentrations (MIC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes) with MIC=1,25–10 μg/mL and 0,0781–0,3125 μg/mL, respectively. As results obtained by MTT test, the extracts didn’t show anticancer activity (IC50>100 μg/L) against tested tumor cell lines: PC-3 (prostate), HT-29 (colon) and HeLa (cervix) in the applied concentration range. For investigation of oxidative stability of CPSU, only 70% ethanol extracts were used. Samples of CPSU (with addition of 250, 500 and 1000 ppm of extracts), as well as control samples (with addition of 200 ppm of BHT and CPSU without any additives), were stored at room temperature for six months. In order to determine the level of oxidative changes, samples were analyzed for peroxide, anisidine value and conjugated dienes and trienes. Based on obtained results, it can be concluded that savory extracts are powerful natural antioxidants that can delay the oxidative deterioration of CPSU and whose effectiveness is comparable to the synthetic antioxidant BHT.
PB  - University of Belgrade
C3  - UniFood Conference
T1  - Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6630
ER  - 

@conference{
author = "Stojićević, Aleksandra and Alimpić Aradski, Ana and Pantić, Milena and Pantelić, Nebojša and Rabrenović, Biljana and Duletić-Laušević, Sonja and Antić, Mališa and Nikšić, Miomir",
year = "2021",
abstract = "Medicinal plants represent a rich source of phytochemicals, many of which possess diverse biological activities, including antioxidants. The objectives of this study were to assess antioxidant, antibacterial and anticancer activities of savory (Satureja montana L.) ethanolic extracts and to monitor the changes of oxidative status during long-term storage conditions of the cold pressed sunflower oil (CPSU) with the addition of extracts at various concentrations.  Extracts were obtained by Soxhlet extraction and ultrasound-assisted maceration, using 70% and 96% ethanol. Antioxidant activity was evaluated by DPPH and ꞵ-carotene bleaching assays. In both assays, extracts showed remarkable antioxidative properties which were equal or stronger than those of BHT and BHA. Also, regardless of the extraction method and assay applied, the 70% ethanol extracts proved to be more potent than 96% ethanol ones. For antibacterial activity assay, minimal inhibitory concentrations (MIC) were obtained by the microdilution method. Tested Gram-negative bacteria (E. coli and S. typhimurium) were more resistant than Gram-positive (S. aureus and L. monocytogenes) with MIC=1,25–10 μg/mL and 0,0781–0,3125 μg/mL, respectively. As results obtained by MTT test, the extracts didn’t show anticancer activity (IC50>100 μg/L) against tested tumor cell lines: PC-3 (prostate), HT-29 (colon) and HeLa (cervix) in the applied concentration range. For investigation of oxidative stability of CPSU, only 70% ethanol extracts were used. Samples of CPSU (with addition of 250, 500 and 1000 ppm of extracts), as well as control samples (with addition of 200 ppm of BHT and CPSU without any additives), were stored at room temperature for six months. In order to determine the level of oxidative changes, samples were analyzed for peroxide, anisidine value and conjugated dienes and trienes. Based on obtained results, it can be concluded that savory extracts are powerful natural antioxidants that can delay the oxidative deterioration of CPSU and whose effectiveness is comparable to the synthetic antioxidant BHT.",
publisher = "University of Belgrade",
journal = "UniFood Conference",
title = "Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6630"
}

Stojićević, A., Alimpić Aradski, A., Pantić, M., Pantelić, N., Rabrenović, B., Duletić-Laušević, S., Antić, M.,& Nikšić, M.. (2021). Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions. in UniFood Conference
University of Belgrade., 101.
https://hdl.handle.net/21.15107/rcub_agrospace_6630

Stojićević A, Alimpić Aradski A, Pantić M, Pantelić N, Rabrenović B, Duletić-Laušević S, Antić M, Nikšić M. Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions. in UniFood Conference. 2021;:101.
https://hdl.handle.net/21.15107/rcub_agrospace_6630 .

Stojićević, Aleksandra, Alimpić Aradski, Ana, Pantić, Milena, Pantelić, Nebojša, Rabrenović, Biljana, Duletić-Laušević, Sonja, Antić, Mališa, Nikšić, Miomir, "Biological activity of Satureja montana L. ethanolic extracts and their effect on oxidative stability of cold pressed sunflower oil in long-term storage conditions" in UniFood Conference (2021):101,
https://hdl.handle.net/21.15107/rcub_agrospace_6630 .

TY  - CONF
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
AU  - Antić, Mališa
AU  - Solević, Tatjana
AU  - Ilić, Mila
AU  - Novaković, Milan
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2035
PB  - Amer Chemical Soc, Washington
C3  - Abstracts of Papers of the American Chemical Society
T1  - ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil
VL  - 238
UR  - https://hdl.handle.net/21.15107/rcub_cherry_524
ER  - 

@conference{
author = "Jovančićević, Branimir and Vrvić, Miroslav M. and Antić, Mališa and Solević, Tatjana and Ilić, Mila and Novaković, Milan",
year = "2009",
publisher = "Amer Chemical Soc, Washington",
journal = "Abstracts of Papers of the American Chemical Society",
title = "ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil",
volume = "238",
url = "https://hdl.handle.net/21.15107/rcub_cherry_524"
}

Jovančićević, B., Vrvić, M. M., Antić, M., Solević, T., Ilić, M.,& Novaković, M.. (2009). ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in Abstracts of Papers of the American Chemical Society
Amer Chemical Soc, Washington., 238.
https://hdl.handle.net/21.15107/rcub_cherry_524

Jovančićević B, Vrvić MM, Antić M, Solević T, Ilić M, Novaković M. ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in Abstracts of Papers of the American Chemical Society. 2009;238.
https://hdl.handle.net/21.15107/rcub_cherry_524 .

Jovančićević, Branimir, Vrvić, Miroslav M., Antić, Mališa, Solević, Tatjana, Ilić, Mila, Novaković, Milan, "ENVR 54-Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil" in Abstracts of Papers of the American Chemical Society, 238 (2009),
https://hdl.handle.net/21.15107/rcub_cherry_524 .

TY  - CONF
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
AU  - Antić, Mališa
AU  - Solević, T.
AU  - Ilić, M.
AU  - Novaković, M.
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1844
AB  - Bioremediation potential of bacteria and fungi isolated from sediment samples from an alluvial formation of the Danube (wastewater channel) of the Pancevo Industrial Zone (Serbia) was investigated earlier. (Ilic M., Antic M., Antic V., Schwarzbauer J., Vrvic M. and Jovancicevic B., Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. Environmental Chemistry Letters (submitted)) In this paper, results concerning bioremediation potential of bacteria and fungi isolated from soil samples will be presented. Total isolated microorganisms were divided into three parts and sown in an appropriate medium. Actidione fungicide was added to one innoculum so that only bacteria propagated in it. Streptomycin antibiotic was added to a second innoculum, wherein fungi propagated. The third portion (with no additives) contained consortium of fungi and bacteria. Paraffinic petroleum, in inorganic medium in phosphate puffer, was a substrate for assessment of bioremediation potential of the mentioned microorganisms. The experiments of the simulated oil biodegradation lasted 30, 60 and 90 days. Extracts were isolated from the samples with chloroform liquid-liquid extraction. Extracts were fractionated by column chromatography. Alkane fraction was analyzed by gas chromatography-mass spectrometry (GC-MS) techniques.
PB  - 238th National Meeting and Exposition of the American Chemical Society, ACS 2009
C3  - ACS National Meeting Book of Abstracts
T1  - Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil
UR  - https://hdl.handle.net/21.15107/rcub_cherry_111
ER  - 

@conference{
author = "Jovančićević, Branimir and Vrvić, Miroslav M. and Antić, Mališa and Solević, T. and Ilić, M. and Novaković, M.",
year = "2009",
abstract = "Bioremediation potential of bacteria and fungi isolated from sediment samples from an alluvial formation of the Danube (wastewater channel) of the Pancevo Industrial Zone (Serbia) was investigated earlier. (Ilic M., Antic M., Antic V., Schwarzbauer J., Vrvic M. and Jovancicevic B., Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. Environmental Chemistry Letters (submitted)) In this paper, results concerning bioremediation potential of bacteria and fungi isolated from soil samples will be presented. Total isolated microorganisms were divided into three parts and sown in an appropriate medium. Actidione fungicide was added to one innoculum so that only bacteria propagated in it. Streptomycin antibiotic was added to a second innoculum, wherein fungi propagated. The third portion (with no additives) contained consortium of fungi and bacteria. Paraffinic petroleum, in inorganic medium in phosphate puffer, was a substrate for assessment of bioremediation potential of the mentioned microorganisms. The experiments of the simulated oil biodegradation lasted 30, 60 and 90 days. Extracts were isolated from the samples with chloroform liquid-liquid extraction. Extracts were fractionated by column chromatography. Alkane fraction was analyzed by gas chromatography-mass spectrometry (GC-MS) techniques.",
publisher = "238th National Meeting and Exposition of the American Chemical Society, ACS 2009",
journal = "ACS National Meeting Book of Abstracts",
title = "Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil",
url = "https://hdl.handle.net/21.15107/rcub_cherry_111"
}

Jovančićević, B., Vrvić, M. M., Antić, M., Solević, T., Ilić, M.,& Novaković, M.. (2009). Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in ACS National Meeting Book of Abstracts
238th National Meeting and Exposition of the American Chemical Society, ACS 2009..
https://hdl.handle.net/21.15107/rcub_cherry_111

Jovančićević B, Vrvić MM, Antić M, Solević T, Ilić M, Novaković M. Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil. in ACS National Meeting Book of Abstracts. 2009;.
https://hdl.handle.net/21.15107/rcub_cherry_111 .

Jovančićević, Branimir, Vrvić, Miroslav M., Antić, Mališa, Solević, T., Ilić, M., Novaković, M., "Investigation of bioremediation potential of bacteria and fungi for petroleum degradation in soil" in ACS National Meeting Book of Abstracts (2009),
https://hdl.handle.net/21.15107/rcub_cherry_111 .

TY  - JOUR
AU  - Antić, Vesna
AU  - Vucković, Marija V.
AU  - Dojčinović, Biljana
AU  - Antić, Mališa
AU  - Barać, Miroljub
AU  - Govedarica, Milutin N.
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1801
AB  - The modality of the incorporation of silanol-terminated siloxanes into copolymers with butylene terephthalate were investigated and determined. Besides siloxanes, the other participants in this well-known copolymerization, called the catalyzed two-step transesterification/polycondensation reaction, are dimethyl terephthalate and 1,4-butanediol. The employed approach to this problem was to exactly examine all the possibilities of the reactions between particular pairs of reactants, as well as of single reactants. The final results were, firstly, that reactions between siloxanes and dimethyl terephthalate did not occur and, secondly, that the only possibility for silanol-terminated siloxanes to be incorporated into the copolymers was by means of their condensation with 1,4-butanediol. This means that siloxanes are incorporated into the copolymer by ether and not by ester linkages.
PB  - Elsevier, Amsterdam
T2  - Reactive & Functional Polymers
T1  - About the mode of incorporation of silanol-terminated polysiloxanes into butylene terephthalate-b-dimethylsiloxane copolymers
EP  - 860
IS  - 4
SP  - 851
VL  - 68
DO  - 10.1016/j.reactfunctpolym.2007.12.004
ER  - 

@article{
author = "Antić, Vesna and Vucković, Marija V. and Dojčinović, Biljana and Antić, Mališa and Barać, Miroljub and Govedarica, Milutin N.",
year = "2008",
abstract = "The modality of the incorporation of silanol-terminated siloxanes into copolymers with butylene terephthalate were investigated and determined. Besides siloxanes, the other participants in this well-known copolymerization, called the catalyzed two-step transesterification/polycondensation reaction, are dimethyl terephthalate and 1,4-butanediol. The employed approach to this problem was to exactly examine all the possibilities of the reactions between particular pairs of reactants, as well as of single reactants. The final results were, firstly, that reactions between siloxanes and dimethyl terephthalate did not occur and, secondly, that the only possibility for silanol-terminated siloxanes to be incorporated into the copolymers was by means of their condensation with 1,4-butanediol. This means that siloxanes are incorporated into the copolymer by ether and not by ester linkages.",
publisher = "Elsevier, Amsterdam",
journal = "Reactive & Functional Polymers",
title = "About the mode of incorporation of silanol-terminated polysiloxanes into butylene terephthalate-b-dimethylsiloxane copolymers",
pages = "860-851",
number = "4",
volume = "68",
doi = "10.1016/j.reactfunctpolym.2007.12.004"
}

Antić, V., Vucković, M. V., Dojčinović, B., Antić, M., Barać, M.,& Govedarica, M. N.. (2008). About the mode of incorporation of silanol-terminated polysiloxanes into butylene terephthalate-b-dimethylsiloxane copolymers. in Reactive & Functional Polymers
Elsevier, Amsterdam., 68(4), 851-860.
https://doi.org/10.1016/j.reactfunctpolym.2007.12.004

Antić V, Vucković MV, Dojčinović B, Antić M, Barać M, Govedarica MN. About the mode of incorporation of silanol-terminated polysiloxanes into butylene terephthalate-b-dimethylsiloxane copolymers. in Reactive & Functional Polymers. 2008;68(4):851-860.
doi:10.1016/j.reactfunctpolym.2007.12.004 .

Antić, Vesna, Vucković, Marija V., Dojčinović, Biljana, Antić, Mališa, Barać, Miroljub, Govedarica, Milutin N., "About the mode of incorporation of silanol-terminated polysiloxanes into butylene terephthalate-b-dimethylsiloxane copolymers" in Reactive & Functional Polymers, 68, no. 4 (2008):851-860,
https://doi.org/10.1016/j.reactfunctpolym.2007.12.004 . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Vrvić, Miroslav M.
AU  - Ilić, M.
AU  - Novaković, M.
AU  - Saheed, R.M.
AU  - Schwarzbauer, Jan
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1808
AB  - The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P-1-P-12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P-8-P-12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Transformation of a petroleum pollutant during soil bioremediation experiments
EP  - 583
IS  - 5
SP  - 577
VL  - 73
DO  - 10.2298/JSC0805577J
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Vrvić, Miroslav M. and Ilić, M. and Novaković, M. and Saheed, R.M. and Schwarzbauer, Jan",
year = "2008",
abstract = "The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P-1-P-12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P-8-P-12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Transformation of a petroleum pollutant during soil bioremediation experiments",
pages = "583-577",
number = "5",
volume = "73",
doi = "10.2298/JSC0805577J"
}

Jovančićević, B., Antić, M., Vrvić, M. M., Ilić, M., Novaković, M., Saheed, R.M.,& Schwarzbauer, J.. (2008). Transformation of a petroleum pollutant during soil bioremediation experiments. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 73(5), 577-583.
https://doi.org/10.2298/JSC0805577J

Jovančićević B, Antić M, Vrvić MM, Ilić M, Novaković M, Saheed R, Schwarzbauer J. Transformation of a petroleum pollutant during soil bioremediation experiments. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2008;73(5):577-583.
doi:10.2298/JSC0805577J .

Jovančićević, Branimir, Antić, Mališa, Vrvić, Miroslav M., Ilić, M., Novaković, M., Saheed, R.M., Schwarzbauer, Jan, "Transformation of a petroleum pollutant during soil bioremediation experiments" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 73, no. 5 (2008):577-583,
https://doi.org/10.2298/JSC0805577J . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Pavlović, I.
AU  - Vrvić, Miroslav M.
AU  - Beskoski, Vladimir
AU  - Kronimus, Alexander
AU  - Schwarzbauer, Jan
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1632
AB  - This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pančevo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P1-P12 samples). Organic substance was extracted by Soxhlet's method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P1. Pristane and phytane occurred in sample P8, and in even higher quantities in the final sample P12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P1 the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P8 and P12 because of the reduced concentration. The observed changes in the alkane fractions' compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.
T2  - Water, Air, and Soil Pollution
T1  - Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments
EP  - 307
IS  - 1-4
SP  - 299
VL  - 190
DO  - 10.1007/s11270-007-9601-z
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Pavlović, I. and Vrvić, Miroslav M. and Beskoski, Vladimir and Kronimus, Alexander and Schwarzbauer, Jan",
year = "2008",
abstract = "This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pančevo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P1-P12 samples). Organic substance was extracted by Soxhlet's method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P1. Pristane and phytane occurred in sample P8, and in even higher quantities in the final sample P12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P1 the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P8 and P12 because of the reduced concentration. The observed changes in the alkane fractions' compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.",
journal = "Water, Air, and Soil Pollution",
title = "Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments",
pages = "307-299",
number = "1-4",
volume = "190",
doi = "10.1007/s11270-007-9601-z"
}

Jovančićević, B., Antić, M., Pavlović, I., Vrvić, M. M., Beskoski, V., Kronimus, A.,& Schwarzbauer, J.. (2008). Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments. in Water, Air, and Soil Pollution, 190(1-4), 299-307.
https://doi.org/10.1007/s11270-007-9601-z

Jovančićević B, Antić M, Pavlović I, Vrvić MM, Beskoski V, Kronimus A, Schwarzbauer J. Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments. in Water, Air, and Soil Pollution. 2008;190(1-4):299-307.
doi:10.1007/s11270-007-9601-z .

Jovančićević, Branimir, Antić, Mališa, Pavlović, I., Vrvić, Miroslav M., Beskoski, Vladimir, Kronimus, Alexander, Schwarzbauer, Jan, "Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments" in Water, Air, and Soil Pollution, 190, no. 1-4 (2008):299-307,
https://doi.org/10.1007/s11270-007-9601-z . .

TY  - CONF
AU  - Ilić, Mila
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Ljaljević-Grbić, Milica
AU  - Cvijan, Mirko
AU  - Vrvić, Miroslav M.
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1597
PB  - Elsevier Science Bv, Amsterdam
C3  - Journal of Biotechnology
T1  - Surface water microorganisms degrade dominantly petroleum hydrocarbons
EP  - S150
IS  - 2
SP  - S150
VL  - 131
DO  - 10.1016/j.jbiotec.2007.07.865
ER  - 

@conference{
author = "Ilić, Mila and Jovančićević, Branimir and Antić, Mališa and Ljaljević-Grbić, Milica and Cvijan, Mirko and Vrvić, Miroslav M.",
year = "2007",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Surface water microorganisms degrade dominantly petroleum hydrocarbons",
pages = "S150-S150",
number = "2",
volume = "131",
doi = "10.1016/j.jbiotec.2007.07.865"
}

Ilić, M., Jovančićević, B., Antić, M., Ljaljević-Grbić, M., Cvijan, M.,& Vrvić, M. M.. (2007). Surface water microorganisms degrade dominantly petroleum hydrocarbons. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 131(2), S150-S150.
https://doi.org/10.1016/j.jbiotec.2007.07.865

Ilić M, Jovančićević B, Antić M, Ljaljević-Grbić M, Cvijan M, Vrvić MM. Surface water microorganisms degrade dominantly petroleum hydrocarbons. in Journal of Biotechnology. 2007;131(2):S150-S150.
doi:10.1016/j.jbiotec.2007.07.865 .

Ilić, Mila, Jovančićević, Branimir, Antić, Mališa, Ljaljević-Grbić, Milica, Cvijan, Mirko, Vrvić, Miroslav M., "Surface water microorganisms degrade dominantly petroleum hydrocarbons" in Journal of Biotechnology, 131, no. 2 (2007):S150-S150,
https://doi.org/10.1016/j.jbiotec.2007.07.865 . .

TY  - JOUR
AU  - Antić, Mališa
AU  - Jovančićević, Branimir
AU  - Ilić, Mila
AU  - Vrvić, Miroslav M.
AU  - Schwarzbauer, Jan
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1351
AB  - BACKGROUND: , Aims and Scope. It is well known that the composition of petroleum or some of its processing products changes in the environment mostly under the influence of microorganisms. A series of experiments was conducted in order to define the optimum conditions for an efficient biodegradation of petroleum pollutant, or bioremediation of different segments of the environment. The aim of these investigations was to show to what extent the hydrocarbons of a petroleum pollutant are degraded by microbial cultures which were isolated as dominant microorganisms from a surface water of a wastewater canal of an oil refinery and a nitrogen plant. Biodegradation experiments were conducted on one paraffinic, and one naphthenic type of petroleum during a three month period under aerobic conditions, varying the following parameters: Inorganic (Kp) or an organic medium (Bh) with or without exposition to light. Methods. Microorganisms were analyzed in a surface water sample from a canal (Pancevo, Serbia), into which wastewater from an oil refinery and a nitrogen plant is released. The consortia of microorganisms were isolated from the water sample (most abundant species: Phormidium foveolarum - filamentous Cyanobacteria, blue-green algae and Achanthes minutissima, diatoms, algae). The simulation experiments of biodegradation were conducted with the biomass suspension and crude oils Sirakovo (Sir, paraffinic type) and Velebit (Ve, naphthenic type). After a three month period, organic substance was extracted by means of chloroform. In the extracts, the content of saturated hydrocarbons, aromatic hydrocarbons, alcohols and fatty acids was determined (the group composition). n-Alkanes and isoprenoid aliphatic alkanes, pristane and phytane, in the aliphatic fractions, were analyzed using gas chromatography (GC). Total isoprenoid aliphatic alkanes and polycyclic alkanes of sterane and triterpane types were analyzed by GC-MS. Results and Discussion. Paraffinic type petroleums have a significant loss of saturated hydrocarbons. For naphthenic type petroleum, such a trend has not been observed. The most intensive degradation of n-alkanes and isoprenoid aliphatic alkanes (in paraffinic oil) and isoprenoids (in naphthenic oil) was observed using the inorganic medium Kp in the light; the microbial conversion is somewhat lower with Kp in the dark; with organic medium Bh in the light the degradation is of low intensity; with the same medium in the dark the degradation is hardly to be seen. Steranes and triterpanes were not affected by microbial degradation under the conditions used in our experiments. Obviously, the petroleum biodegradation was restricted to the acyclic aliphatics (n-alkanes and isoprenoids). Conclusion. Phormidium foveolarum (filamentous Cyanobacteria - blue-green algae) and Achanthes minutissima (diatoms, algae), microbial cultures isolated as dominant algae from a surface water in a wastewater canal of an oil refinery and a nitrogen plant, have degradable effects dominantly involving petroleum hydocarbons. Petroleum microbiological degradation is more intensive when inorganic medium (in the light) is applied. Having in mind that the inorganic pollutants have been released into the canal as well, this medium reflects more the natural environmental conditions. Polycyclic alkanes of sterane and triterpane type, in spite of the fact that these compounds could be degraded, have remained unchanged regarding abundance and distribution. Since this is the case even for naphthenic type petroleum (which is depleted in n-alkanes), it can be concluded that the biodegradation of petroleum type pollutants, under natural conditions, will be restrained to the n-alkane and isoprenoid degradation. Recommendation and Outlook. Performed experiments and simulations of petroleum microbiological degradation may serve for the prediction of the fate of petroleum type pollutants, as well as for definition of conditions for bioremediation of some environmental segments.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Petroleum pollutant degradation by surface water microorganisms
EP  - 327
IS  - 5
SP  - 320
VL  - 13
DO  - 10.1065/espr2006.03.296
ER  - 

@article{
author = "Antić, Mališa and Jovančićević, Branimir and Ilić, Mila and Vrvić, Miroslav M. and Schwarzbauer, Jan",
year = "2006",
abstract = "BACKGROUND: , Aims and Scope. It is well known that the composition of petroleum or some of its processing products changes in the environment mostly under the influence of microorganisms. A series of experiments was conducted in order to define the optimum conditions for an efficient biodegradation of petroleum pollutant, or bioremediation of different segments of the environment. The aim of these investigations was to show to what extent the hydrocarbons of a petroleum pollutant are degraded by microbial cultures which were isolated as dominant microorganisms from a surface water of a wastewater canal of an oil refinery and a nitrogen plant. Biodegradation experiments were conducted on one paraffinic, and one naphthenic type of petroleum during a three month period under aerobic conditions, varying the following parameters: Inorganic (Kp) or an organic medium (Bh) with or without exposition to light. Methods. Microorganisms were analyzed in a surface water sample from a canal (Pancevo, Serbia), into which wastewater from an oil refinery and a nitrogen plant is released. The consortia of microorganisms were isolated from the water sample (most abundant species: Phormidium foveolarum - filamentous Cyanobacteria, blue-green algae and Achanthes minutissima, diatoms, algae). The simulation experiments of biodegradation were conducted with the biomass suspension and crude oils Sirakovo (Sir, paraffinic type) and Velebit (Ve, naphthenic type). After a three month period, organic substance was extracted by means of chloroform. In the extracts, the content of saturated hydrocarbons, aromatic hydrocarbons, alcohols and fatty acids was determined (the group composition). n-Alkanes and isoprenoid aliphatic alkanes, pristane and phytane, in the aliphatic fractions, were analyzed using gas chromatography (GC). Total isoprenoid aliphatic alkanes and polycyclic alkanes of sterane and triterpane types were analyzed by GC-MS. Results and Discussion. Paraffinic type petroleums have a significant loss of saturated hydrocarbons. For naphthenic type petroleum, such a trend has not been observed. The most intensive degradation of n-alkanes and isoprenoid aliphatic alkanes (in paraffinic oil) and isoprenoids (in naphthenic oil) was observed using the inorganic medium Kp in the light; the microbial conversion is somewhat lower with Kp in the dark; with organic medium Bh in the light the degradation is of low intensity; with the same medium in the dark the degradation is hardly to be seen. Steranes and triterpanes were not affected by microbial degradation under the conditions used in our experiments. Obviously, the petroleum biodegradation was restricted to the acyclic aliphatics (n-alkanes and isoprenoids). Conclusion. Phormidium foveolarum (filamentous Cyanobacteria - blue-green algae) and Achanthes minutissima (diatoms, algae), microbial cultures isolated as dominant algae from a surface water in a wastewater canal of an oil refinery and a nitrogen plant, have degradable effects dominantly involving petroleum hydocarbons. Petroleum microbiological degradation is more intensive when inorganic medium (in the light) is applied. Having in mind that the inorganic pollutants have been released into the canal as well, this medium reflects more the natural environmental conditions. Polycyclic alkanes of sterane and triterpane type, in spite of the fact that these compounds could be degraded, have remained unchanged regarding abundance and distribution. Since this is the case even for naphthenic type petroleum (which is depleted in n-alkanes), it can be concluded that the biodegradation of petroleum type pollutants, under natural conditions, will be restrained to the n-alkane and isoprenoid degradation. Recommendation and Outlook. Performed experiments and simulations of petroleum microbiological degradation may serve for the prediction of the fate of petroleum type pollutants, as well as for definition of conditions for bioremediation of some environmental segments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Petroleum pollutant degradation by surface water microorganisms",
pages = "327-320",
number = "5",
volume = "13",
doi = "10.1065/espr2006.03.296"
}

Antić, M., Jovančićević, B., Ilić, M., Vrvić, M. M.,& Schwarzbauer, J.. (2006). Petroleum pollutant degradation by surface water microorganisms. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 13(5), 320-327.
https://doi.org/10.1065/espr2006.03.296

Antić M, Jovančićević B, Ilić M, Vrvić MM, Schwarzbauer J. Petroleum pollutant degradation by surface water microorganisms. in Environmental Science and Pollution Research. 2006;13(5):320-327.
doi:10.1065/espr2006.03.296 .

Antić, Mališa, Jovančićević, Branimir, Ilić, Mila, Vrvić, Miroslav M., Schwarzbauer, Jan, "Petroleum pollutant degradation by surface water microorganisms" in Environmental Science and Pollution Research, 13, no. 5 (2006):320-327,
https://doi.org/10.1065/espr2006.03.296 . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Solević, TM
AU  - Vrvić, Miroslav M.
AU  - Kronimus, Alexander
AU  - Schwarzbauer, Jan
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1010
AB  - BACKGROUND: , Aims and Scope. In oil spill investigations, one of the most important steps is a proper choice of approaches that imply an investigation of samples taken from different sedimentary environments, samples of oil contaminants taken in different periods of time and samples taken at different distances from the oil spill. In all these cases, conclusion on the influence of the environment, microorganisms or migration on the oil contaminants' composition can be drawn from the comparison of chemical compositions of the investigated contaminants. However, in case of water contaminants, it is very important to define which part of organic matter has been analyzed. Namely, previous investigations showed that there were some differences in chemical composition of the same oil contaminant depending on the intensity of its contact with ground water. The aim of this work is to define more precisely the interactions between oil contaminant and water, i.e. the influence of the intensity of interaction between the oil contaminant and water on its chemical composition. The study was based on a comparison of four fractionated extracts of an oil pollutant, after they had been analyzed in details. Methods. Oil polluted surface water (wastewater canal, Pancevo, Serbia) was investigated. The study was based on a comparison of four extracts of an oil contaminant: extract I (decanted part), and extracts 2, 3 and 4 (extracted by shaking for 1 minute, 5 minutes and 24 hours, respectively). The fractionated extracts were saponified with a solution of KOH in methanol, and neutralized with 10% hydrochloric acid. The products were dissolved in a mixture of dichloromethane and hexane, and individually fractionated by column chromatography on alumina and silica gel (saturated hydrocarbon, aromatic, alcohol and fatty acid fractions). n-Alkanes and isoprenoid aliphatic alkanes, polycyclic alkanes of sterane and triterpane types, alcohols and fatty acids were analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). delta C-13(PDB) values of individual n-alkanes in the aliphatic fractions were determined using gas chromatography-isotope ratio monitoring-mass spectrometry (GC-irmMS). Results and discussion. Extracts 1 and 2 are characterized by uniform distribution of n-alkanes, whereas extract 3 is characterized by an even-numbered members dominating the odd-ones, and extract 4 showed a bimodal distribution. Extract I is characterized by the least negative delta C-13(PDB) values of C-19-C-26 n-alkanes. Sterane and triterpane analysis confirmed that all extracts originated from the same oil contaminant. n-Fatty acids, C-19-C-24, in all extracts are very low, being somewhat higher in extract 4. Even-numbered n-alcohols, C-12-C-16, were identified in the highest concentration in extract 3. It was assumed that algae were responsible for the composition of extract 3. Furthermore, a possible reason for higher concentrations of C-19-C-26 n-alkanes and C-19-C-24 fatty acids in extract 4 is the formation of inclusion compounds with colloidal micelles formed between the oil contaminant's NSO-compounds and water. Conclusion. It was undoubtedly confirmed that there were specific differences in the compositions of the different extracts depending on the intensity of the interaction between the oil contaminant and the surface water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Investigation of interactions between surface water and petroleum-type pollutants
EP  - 212
IS  - 4
SP  - 205
VL  - 12
DO  - 10.1065/espr2004.12.229
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Solević, TM and Vrvić, Miroslav M. and Kronimus, Alexander and Schwarzbauer, Jan",
year = "2005",
abstract = "BACKGROUND: , Aims and Scope. In oil spill investigations, one of the most important steps is a proper choice of approaches that imply an investigation of samples taken from different sedimentary environments, samples of oil contaminants taken in different periods of time and samples taken at different distances from the oil spill. In all these cases, conclusion on the influence of the environment, microorganisms or migration on the oil contaminants' composition can be drawn from the comparison of chemical compositions of the investigated contaminants. However, in case of water contaminants, it is very important to define which part of organic matter has been analyzed. Namely, previous investigations showed that there were some differences in chemical composition of the same oil contaminant depending on the intensity of its contact with ground water. The aim of this work is to define more precisely the interactions between oil contaminant and water, i.e. the influence of the intensity of interaction between the oil contaminant and water on its chemical composition. The study was based on a comparison of four fractionated extracts of an oil pollutant, after they had been analyzed in details. Methods. Oil polluted surface water (wastewater canal, Pancevo, Serbia) was investigated. The study was based on a comparison of four extracts of an oil contaminant: extract I (decanted part), and extracts 2, 3 and 4 (extracted by shaking for 1 minute, 5 minutes and 24 hours, respectively). The fractionated extracts were saponified with a solution of KOH in methanol, and neutralized with 10% hydrochloric acid. The products were dissolved in a mixture of dichloromethane and hexane, and individually fractionated by column chromatography on alumina and silica gel (saturated hydrocarbon, aromatic, alcohol and fatty acid fractions). n-Alkanes and isoprenoid aliphatic alkanes, polycyclic alkanes of sterane and triterpane types, alcohols and fatty acids were analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). delta C-13(PDB) values of individual n-alkanes in the aliphatic fractions were determined using gas chromatography-isotope ratio monitoring-mass spectrometry (GC-irmMS). Results and discussion. Extracts 1 and 2 are characterized by uniform distribution of n-alkanes, whereas extract 3 is characterized by an even-numbered members dominating the odd-ones, and extract 4 showed a bimodal distribution. Extract I is characterized by the least negative delta C-13(PDB) values of C-19-C-26 n-alkanes. Sterane and triterpane analysis confirmed that all extracts originated from the same oil contaminant. n-Fatty acids, C-19-C-24, in all extracts are very low, being somewhat higher in extract 4. Even-numbered n-alcohols, C-12-C-16, were identified in the highest concentration in extract 3. It was assumed that algae were responsible for the composition of extract 3. Furthermore, a possible reason for higher concentrations of C-19-C-26 n-alkanes and C-19-C-24 fatty acids in extract 4 is the formation of inclusion compounds with colloidal micelles formed between the oil contaminant's NSO-compounds and water. Conclusion. It was undoubtedly confirmed that there were specific differences in the compositions of the different extracts depending on the intensity of the interaction between the oil contaminant and the surface water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Investigation of interactions between surface water and petroleum-type pollutants",
pages = "212-205",
number = "4",
volume = "12",
doi = "10.1065/espr2004.12.229"
}

Jovančićević, B., Antić, M., Solević, T., Vrvić, M. M., Kronimus, A.,& Schwarzbauer, J.. (2005). Investigation of interactions between surface water and petroleum-type pollutants. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 12(4), 205-212.
https://doi.org/10.1065/espr2004.12.229

Jovančićević B, Antić M, Solević T, Vrvić MM, Kronimus A, Schwarzbauer J. Investigation of interactions between surface water and petroleum-type pollutants. in Environmental Science and Pollution Research. 2005;12(4):205-212.
doi:10.1065/espr2004.12.229 .

Jovančićević, Branimir, Antić, Mališa, Solević, TM, Vrvić, Miroslav M., Kronimus, Alexander, Schwarzbauer, Jan, "Investigation of interactions between surface water and petroleum-type pollutants" in Environmental Science and Pollution Research, 12, no. 4 (2005):205-212,
https://doi.org/10.1065/espr2004.12.229 . .