TY  - JOUR
AU  - Vidović, Nada
AU  - Antić, Vesna
AU  - Schwarzbauer, Jan
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6346
AB  - Reliable identification and quantification of water-soluble synthetic polymers (WSSP), such as polyethyleneimine (PEI), is crucial for understanding their potential impact on certain ecological environments. Therefore, this study proposes an analytical method combining on-line and off-line pyrolysis GC/MS to investigate the pyrolytic degradation. mechanism of branched PEI and develop a calibration method using specific pyrolysis. products, including methylpyrazine, dimethylpyrazine, ethylmethylpyrazine, trimethylpyrazine, methylpropylpyrazine and ethyldimethylpyrazine, for the. quantification of PEI in environmental wastewater samples. The developed analytical. method shows adequate linearity for all molecular ions of the specific pyrolysis. products, with a determination coefficient ranging from 0.95 to 0.98 for the range of 10–500 μg PEI. The limit of quantification for this method lies between 1 and 10 μg/L of PEI. The accuracy (absolute recovery 71–95%) and reproducibility (RSD < 30%) were. evaluated by multiple spiking/recovery experiments at a PEI level of 500 μg. Using this. method for samples collected from different wastewater treatment plants in Germany. reveals a significant emission of PEI to the aquatic environment with concentrations. ranging from 0.08 mg L-1–0.55 mg L-1, and thus demonstrating its applicability in. environmental studies. To the best of our knowledge, this is the first report on the. occurrence of PEI in environmental samples. © 2023 Elsevier B.V.
T2  - Journal of Analytical and Applied Pyrolysis
T2  - Journal of Analytical and Applied Pyrolysis
T1  - Determination of the water-soluble polymer poly(ethyleneimine) (PEI) in wastewater by continuous-flow off-line pyrolysis GC/MS
VL  - 172
DO  - 10.1016/j.jaap.2023.106001
ER  - 

@article{
author = "Vidović, Nada and Antić, Vesna and Schwarzbauer, Jan",
year = "2023",
abstract = "Reliable identification and quantification of water-soluble synthetic polymers (WSSP), such as polyethyleneimine (PEI), is crucial for understanding their potential impact on certain ecological environments. Therefore, this study proposes an analytical method combining on-line and off-line pyrolysis GC/MS to investigate the pyrolytic degradation. mechanism of branched PEI and develop a calibration method using specific pyrolysis. products, including methylpyrazine, dimethylpyrazine, ethylmethylpyrazine, trimethylpyrazine, methylpropylpyrazine and ethyldimethylpyrazine, for the. quantification of PEI in environmental wastewater samples. The developed analytical. method shows adequate linearity for all molecular ions of the specific pyrolysis. products, with a determination coefficient ranging from 0.95 to 0.98 for the range of 10–500 μg PEI. The limit of quantification for this method lies between 1 and 10 μg/L of PEI. The accuracy (absolute recovery 71–95%) and reproducibility (RSD < 30%) were. evaluated by multiple spiking/recovery experiments at a PEI level of 500 μg. Using this. method for samples collected from different wastewater treatment plants in Germany. reveals a significant emission of PEI to the aquatic environment with concentrations. ranging from 0.08 mg L-1–0.55 mg L-1, and thus demonstrating its applicability in. environmental studies. To the best of our knowledge, this is the first report on the. occurrence of PEI in environmental samples. © 2023 Elsevier B.V.",
journal = "Journal of Analytical and Applied Pyrolysis, Journal of Analytical and Applied Pyrolysis",
title = "Determination of the water-soluble polymer poly(ethyleneimine) (PEI) in wastewater by continuous-flow off-line pyrolysis GC/MS",
volume = "172",
doi = "10.1016/j.jaap.2023.106001"
}

Vidović, N., Antić, V.,& Schwarzbauer, J.. (2023). Determination of the water-soluble polymer poly(ethyleneimine) (PEI) in wastewater by continuous-flow off-line pyrolysis GC/MS. in Journal of Analytical and Applied Pyrolysis, 172.
https://doi.org/10.1016/j.jaap.2023.106001

Vidović N, Antić V, Schwarzbauer J. Determination of the water-soluble polymer poly(ethyleneimine) (PEI) in wastewater by continuous-flow off-line pyrolysis GC/MS. in Journal of Analytical and Applied Pyrolysis. 2023;172.
doi:10.1016/j.jaap.2023.106001 .

Vidović, Nada, Antić, Vesna, Schwarzbauer, Jan, "Determination of the water-soluble polymer poly(ethyleneimine) (PEI) in wastewater by continuous-flow off-line pyrolysis GC/MS" in Journal of Analytical and Applied Pyrolysis, 172 (2023),
https://doi.org/10.1016/j.jaap.2023.106001 . .

TY  - JOUR
AU  - al Sandouk-Lincke, Nadia Anna
AU  - Schwarzbauer, Jan
AU  - Antić, Vesna
AU  - Antić, Mališa
AU  - Caase, Jana
AU  - Gruenelt, Sabrina
AU  - Ressing, Katharina
AU  - Littke, Ralf
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3686
AB  - Three different synthetic polymers commonly used in drilling fluids (carboxymethyl cellulose - CMC, hydroxyethyl cellulose - HEC and polyacrylamide - PAA) were analysed by off-line-pyrolysis-gas chromatography-mass spectrometry (off-line-Py-GC-MS). The aim of this study was to determine specific environmental marker compounds for the identification of contamination due to drilling activities. In a first step, reference materials of the main constituents of commonly applied water-based drilling fluids were purchased and analysed to identify potential indicator substances. For each polymer a set of two to three specific pyrolysis products was determined. Afterwards, four CMC, one HEC and two PAA based drilling fluids were pyrolysed in order to retrieve the previously identified compounds in drilling fluid mixtures. All indicator compounds were identified. In a third step, spiking experiments on drill cutting samples of various depths of one well proved the traceability of the applied polymers and verified the applicability of the indicator systems in terms of sensitivity and specificity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Organic Geochemistry
T1  - Off-line-pyrolysis-gas chromatography-mass spectrometry analyses of drilling fluids and drill cuttings - Identification of potential environmental marker substances
EP  - 28
SP  - 17
VL  - 88
DO  - 10.1016/j.orggeochem.2015.08.001
ER  - 

@article{
author = "al Sandouk-Lincke, Nadia Anna and Schwarzbauer, Jan and Antić, Vesna and Antić, Mališa and Caase, Jana and Gruenelt, Sabrina and Ressing, Katharina and Littke, Ralf",
year = "2015",
abstract = "Three different synthetic polymers commonly used in drilling fluids (carboxymethyl cellulose - CMC, hydroxyethyl cellulose - HEC and polyacrylamide - PAA) were analysed by off-line-pyrolysis-gas chromatography-mass spectrometry (off-line-Py-GC-MS). The aim of this study was to determine specific environmental marker compounds for the identification of contamination due to drilling activities. In a first step, reference materials of the main constituents of commonly applied water-based drilling fluids were purchased and analysed to identify potential indicator substances. For each polymer a set of two to three specific pyrolysis products was determined. Afterwards, four CMC, one HEC and two PAA based drilling fluids were pyrolysed in order to retrieve the previously identified compounds in drilling fluid mixtures. All indicator compounds were identified. In a third step, spiking experiments on drill cutting samples of various depths of one well proved the traceability of the applied polymers and verified the applicability of the indicator systems in terms of sensitivity and specificity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Organic Geochemistry",
title = "Off-line-pyrolysis-gas chromatography-mass spectrometry analyses of drilling fluids and drill cuttings - Identification of potential environmental marker substances",
pages = "28-17",
volume = "88",
doi = "10.1016/j.orggeochem.2015.08.001"
}

al Sandouk-Lincke, N. A., Schwarzbauer, J., Antić, V., Antić, M., Caase, J., Gruenelt, S., Ressing, K.,& Littke, R.. (2015). Off-line-pyrolysis-gas chromatography-mass spectrometry analyses of drilling fluids and drill cuttings - Identification of potential environmental marker substances. in Organic Geochemistry
Pergamon-Elsevier Science Ltd, Oxford., 88, 17-28.
https://doi.org/10.1016/j.orggeochem.2015.08.001

al Sandouk-Lincke NA, Schwarzbauer J, Antić V, Antić M, Caase J, Gruenelt S, Ressing K, Littke R. Off-line-pyrolysis-gas chromatography-mass spectrometry analyses of drilling fluids and drill cuttings - Identification of potential environmental marker substances. in Organic Geochemistry. 2015;88:17-28.
doi:10.1016/j.orggeochem.2015.08.001 .

al Sandouk-Lincke, Nadia Anna, Schwarzbauer, Jan, Antić, Vesna, Antić, Mališa, Caase, Jana, Gruenelt, Sabrina, Ressing, Katharina, Littke, Ralf, "Off-line-pyrolysis-gas chromatography-mass spectrometry analyses of drilling fluids and drill cuttings - Identification of potential environmental marker substances" in Organic Geochemistry, 88 (2015):17-28,
https://doi.org/10.1016/j.orggeochem.2015.08.001 . .

TY  - JOUR
AU  - Ramadan, Ali Mohamed Muftah
AU  - Knudsen-Šolević, Tatjana
AU  - Antić, Mališa
AU  - Beskoski, Vladimir
AU  - Vrvić, Miroslav M.
AU  - Schwarzbauer, Jan
AU  - Jovančićević, Branimir
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3329
AB  - It is well known that during biodegradation of oil under natural geological conditions, or oil pollutants in the environment, degradation of hydrocarbons occurs according to a well-defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation (non-stimulated bioremediation, without addition of biomass, nutrient substances and biosurfactant) of soil contaminated with heavy residual fuel oil (mazut) was conducted during a period of 6 months. Low abundance of n-alkanes in the fraction of total saturated hydrocarbons in the initial sample (identification was possible only after concentration by the urea adduction technique) showed that the investigated oil pollutant was at the boundary between the third and the fourth biodegradation level. During the experiment, an intense degradation of phenanthrene and its methyl-, dimethyl- and trimethyl-isomers was not accompanied by the removal of the remaining n-alkanes. The abundance of n-alkanes remained at the initial low level, even at end of the experiment when the pollutant reached one of the highest biodegradation levels. These results showed that the non-stimulated biodegradation of some hydrocarbons, despite their high biodegradability, had not proceeded completely to the end, even at final degradation stages. Under the condition of reduced availability of some hydrocarbons, microorganisms tend to opt for the less biodegradable but more accessible hydrocarbons.
AB  - Dobro je poznato da se u toku biodegradacije nafte u prirodnim geološkim uslovima, ili naftnog zagađivača u životnoj sredini, degradacija ugljovodonika odigrava prema definisanom rasporedu. Na primer, glavne promene u procesu razgradnje normalnih alkana dešavaju se u toku drugog, "blagog", i trećeg, "umerenog", stupnja (na skali biodegradacije od 1 do 10). Prema dosadašnjim istraživanjima, u četvrtom stupnju, kada počinju intenzivne promene fenantrena i njegovih metil izomera, normalni alkani su već u potpunosti uklonjeni. U ovom radu u toku perioda od 6 meseci izvođena je ex situ prirodna bioremedijacija (nestimulisana bioremedijacija bez dodatka biomase, hranljivih sastojaka i biosurfaktanata) zemljišta zagađenog mazutom. Niska obilnost normalnih alkana u frakciji ukupnih zasićenih alkana u početnom uzorku (identifikacija je bila mogućna tek nakon koncentrovanja pomoću karbamida) pokazala je da je ispitivani naftni zagađivač na granici između trećeg i četvrtog stupnja biodegradacije. Tokom eksperimenta, intenzivnu razgradnju fenantrena i njegovih metil, dimetil i trimetil izomera nije pratilo uklanjanje ostatka normalnih alkana. Njihova obilnost je ostala na početnom, niskom nivou i na kraju eksperimenta kada je zagađivač dostigao jedan od najviših stepena biodegradacije. Dobijeni rezultati su pokazali da se razgradnja pojedinih ugljovodonika uprkos njihovoj visokoj biodegradabilnosti ne odigrava do potpunog kraja ni u završnim fazama degradacije. U uslovima njihove smanjene dostupnosti, mikroorganizmi se opredeljuju za teže degradabilne, ali dostupnije ugljovodonike.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)
T1  - Degradabilnost normalnih alkana za vreme ex situ prirodne bioremedijacije zemljišta zagađenog mazutom
EP  - 1043
IS  - 7
SP  - 1035
VL  - 78
DO  - 10.2298/JSC120829106A
ER  - 

@article{
author = "Ramadan, Ali Mohamed Muftah and Knudsen-Šolević, Tatjana and Antić, Mališa and Beskoski, Vladimir and Vrvić, Miroslav M. and Schwarzbauer, Jan and Jovančićević, Branimir",
year = "2013",
abstract = "It is well known that during biodegradation of oil under natural geological conditions, or oil pollutants in the environment, degradation of hydrocarbons occurs according to a well-defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation (non-stimulated bioremediation, without addition of biomass, nutrient substances and biosurfactant) of soil contaminated with heavy residual fuel oil (mazut) was conducted during a period of 6 months. Low abundance of n-alkanes in the fraction of total saturated hydrocarbons in the initial sample (identification was possible only after concentration by the urea adduction technique) showed that the investigated oil pollutant was at the boundary between the third and the fourth biodegradation level. During the experiment, an intense degradation of phenanthrene and its methyl-, dimethyl- and trimethyl-isomers was not accompanied by the removal of the remaining n-alkanes. The abundance of n-alkanes remained at the initial low level, even at end of the experiment when the pollutant reached one of the highest biodegradation levels. These results showed that the non-stimulated biodegradation of some hydrocarbons, despite their high biodegradability, had not proceeded completely to the end, even at final degradation stages. Under the condition of reduced availability of some hydrocarbons, microorganisms tend to opt for the less biodegradable but more accessible hydrocarbons., Dobro je poznato da se u toku biodegradacije nafte u prirodnim geološkim uslovima, ili naftnog zagađivača u životnoj sredini, degradacija ugljovodonika odigrava prema definisanom rasporedu. Na primer, glavne promene u procesu razgradnje normalnih alkana dešavaju se u toku drugog, "blagog", i trećeg, "umerenog", stupnja (na skali biodegradacije od 1 do 10). Prema dosadašnjim istraživanjima, u četvrtom stupnju, kada počinju intenzivne promene fenantrena i njegovih metil izomera, normalni alkani su već u potpunosti uklonjeni. U ovom radu u toku perioda od 6 meseci izvođena je ex situ prirodna bioremedijacija (nestimulisana bioremedijacija bez dodatka biomase, hranljivih sastojaka i biosurfaktanata) zemljišta zagađenog mazutom. Niska obilnost normalnih alkana u frakciji ukupnih zasićenih alkana u početnom uzorku (identifikacija je bila mogućna tek nakon koncentrovanja pomoću karbamida) pokazala je da je ispitivani naftni zagađivač na granici između trećeg i četvrtog stupnja biodegradacije. Tokom eksperimenta, intenzivnu razgradnju fenantrena i njegovih metil, dimetil i trimetil izomera nije pratilo uklanjanje ostatka normalnih alkana. Njihova obilnost je ostala na početnom, niskom nivou i na kraju eksperimenta kada je zagađivač dostigao jedan od najviših stepena biodegradacije. Dobijeni rezultati su pokazali da se razgradnja pojedinih ugljovodonika uprkos njihovoj visokoj biodegradabilnosti ne odigrava do potpunog kraja ni u završnim fazama degradacije. U uslovima njihove smanjene dostupnosti, mikroorganizmi se opredeljuju za teže degradabilne, ali dostupnije ugljovodonike.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut), Degradabilnost normalnih alkana za vreme ex situ prirodne bioremedijacije zemljišta zagađenog mazutom",
pages = "1043-1035",
number = "7",
volume = "78",
doi = "10.2298/JSC120829106A"
}

Ramadan, A. M. M., Knudsen-Šolević, T., Antić, M., Beskoski, V., Vrvić, M. M., Schwarzbauer, J.,& Jovančićević, B.. (2013). Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut). in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 78(7), 1035-1043.
https://doi.org/10.2298/JSC120829106A

Ramadan AMM, Knudsen-Šolević T, Antić M, Beskoski V, Vrvić MM, Schwarzbauer J, Jovančićević B. Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut). in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2013;78(7):1035-1043.
doi:10.2298/JSC120829106A .

Ramadan, Ali Mohamed Muftah, Knudsen-Šolević, Tatjana, Antić, Mališa, Beskoski, Vladimir, Vrvić, Miroslav M., Schwarzbauer, Jan, Jovančićević, Branimir, "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 78, no. 7 (2013):1035-1043,
https://doi.org/10.2298/JSC120829106A . .

TY  - JOUR
AU  - Antić, Vesna
AU  - Antić, Mališa
AU  - Kronimus, Alexander
AU  - Schwarzbauer, Jan
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2920
AB  - 'On-line' pyrolysis coupled with gas chromatography (GC) was performed for quantitative determination of poly(vinylpyrrolidone) (PVP) in wastewater sample. Gas chromatography-mass spectrometry (GC-MS) showed that the main product of pyrolysis of PVP, at high temperatures, was N-vinylpyrrolidone (NVP). After that, different amounts of commercial PVP were pyrolyzed in order to establish the correlation between the amount of generated NVP (its GC peak area) and the initial mass of pyrolyzed PVP. GC-FID analysis was used for construction of the calibration curve and for quantitative determination of PVP. Very good linear correlation was obtained between the area of NVP peak, generated during pyrolysis, and the initial mass of PVP (r2 = 0.998). Further, solutions of known concentration of PVP were prepared in destilled water ('spiked samples'). Spiked samples were preextracted with diethyl ether and n-hexane, after which the water-layer was evaporated and dissolved again in methanol. Analysis of pyrolysates of preextracted spiked samples showed that the 'recovery' of PVP was above 96 mass%. This finding suggested that pre-extraction could be applied to reduce the concentration of organic substances that could also be pyrolyzed, and thus hinder identification and quantitative determination of PVP, without significant loss of polymer. The sample of an industrial wastewater from Pančevo, Serbia was investigated in the last part of this work. The sample was preextracted in the same way as the spiked samples and than pyrolyzed. NVP was identified by GC-MS in obtained pyrolysate, which was the evidence that PVP was present in the wastewater sample. NVP was quantified on the basis of the peak area in GC-FID chromatogram, and than the concentration of PVP in wastewater sample was calculated based on calibration curve. Concentration of PVP in industrial wastewater amounted 2.5 mg/L.
AB  - U ovom radu je primenjena metoda 'on-line' pirolize u sprezi sa gasnom hromatografijom (GC) za kvantitativno određivanje poli(vinilpirolidona) (PVP) u uzorku otpadne vode. Najpre je gasnomhromatografsko-masenospektrometrijskom analizom (GC-MS) utvrđeno da pirolizom PVP na visokim temperaturama kao glavni proizvod degradacije nastaje monomer, N-vinilpirolidon (NVP). Nakon toga su različite količine komercijalnog PVP pirolizovane da bi se uspostavila korelacija između količine nastalog NVP i inicijalne mase pirolizovanog polimera. Za konstruisanje kalibracione krive i kvantitativno određivanje PVP u rastvorima poznate koncentracije u destilovanoj vodi ('spiked samples'), kao i u uzorku otpadne vode, korišćena je GC analiza. Analizom rastvora komercijalnog PVP poznate koncentracije ustanovljeno je da PVP 'recovery', nakon ekstrakcije n-heksanom i dietil-etrom, iznosi iznad 96 mas.%. U poslednjem delu rada ispitan je uzorak industrijske otpadne vode iz Pančeva, Srbija. Pokazano je da je PVP bio prisutan u otpadnoj vodi i da je njegova koncentracija bila daleko iznad uobičajenih koncentracija niskomolekularnih organskih zagađivača.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Quantitative determination of poly(vinylpyrrolidone) by 'On-line' pyrolysis coupled with gas chromatography
T1  - Kvantitativno određivanje poli(vinilpirolidona) primenom 'On-line' pirolize kuplovane sa gasnom hromatografijom
EP  - 364
IS  - 3
SP  - 357
VL  - 66
DO  - 10.2298/HEMIND111114108A
ER  - 

@article{
author = "Antić, Vesna and Antić, Mališa and Kronimus, Alexander and Schwarzbauer, Jan",
year = "2012",
abstract = "'On-line' pyrolysis coupled with gas chromatography (GC) was performed for quantitative determination of poly(vinylpyrrolidone) (PVP) in wastewater sample. Gas chromatography-mass spectrometry (GC-MS) showed that the main product of pyrolysis of PVP, at high temperatures, was N-vinylpyrrolidone (NVP). After that, different amounts of commercial PVP were pyrolyzed in order to establish the correlation between the amount of generated NVP (its GC peak area) and the initial mass of pyrolyzed PVP. GC-FID analysis was used for construction of the calibration curve and for quantitative determination of PVP. Very good linear correlation was obtained between the area of NVP peak, generated during pyrolysis, and the initial mass of PVP (r2 = 0.998). Further, solutions of known concentration of PVP were prepared in destilled water ('spiked samples'). Spiked samples were preextracted with diethyl ether and n-hexane, after which the water-layer was evaporated and dissolved again in methanol. Analysis of pyrolysates of preextracted spiked samples showed that the 'recovery' of PVP was above 96 mass%. This finding suggested that pre-extraction could be applied to reduce the concentration of organic substances that could also be pyrolyzed, and thus hinder identification and quantitative determination of PVP, without significant loss of polymer. The sample of an industrial wastewater from Pančevo, Serbia was investigated in the last part of this work. The sample was preextracted in the same way as the spiked samples and than pyrolyzed. NVP was identified by GC-MS in obtained pyrolysate, which was the evidence that PVP was present in the wastewater sample. NVP was quantified on the basis of the peak area in GC-FID chromatogram, and than the concentration of PVP in wastewater sample was calculated based on calibration curve. Concentration of PVP in industrial wastewater amounted 2.5 mg/L., U ovom radu je primenjena metoda 'on-line' pirolize u sprezi sa gasnom hromatografijom (GC) za kvantitativno određivanje poli(vinilpirolidona) (PVP) u uzorku otpadne vode. Najpre je gasnomhromatografsko-masenospektrometrijskom analizom (GC-MS) utvrđeno da pirolizom PVP na visokim temperaturama kao glavni proizvod degradacije nastaje monomer, N-vinilpirolidon (NVP). Nakon toga su različite količine komercijalnog PVP pirolizovane da bi se uspostavila korelacija između količine nastalog NVP i inicijalne mase pirolizovanog polimera. Za konstruisanje kalibracione krive i kvantitativno određivanje PVP u rastvorima poznate koncentracije u destilovanoj vodi ('spiked samples'), kao i u uzorku otpadne vode, korišćena je GC analiza. Analizom rastvora komercijalnog PVP poznate koncentracije ustanovljeno je da PVP 'recovery', nakon ekstrakcije n-heksanom i dietil-etrom, iznosi iznad 96 mas.%. U poslednjem delu rada ispitan je uzorak industrijske otpadne vode iz Pančeva, Srbija. Pokazano je da je PVP bio prisutan u otpadnoj vodi i da je njegova koncentracija bila daleko iznad uobičajenih koncentracija niskomolekularnih organskih zagađivača.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Quantitative determination of poly(vinylpyrrolidone) by 'On-line' pyrolysis coupled with gas chromatography, Kvantitativno određivanje poli(vinilpirolidona) primenom 'On-line' pirolize kuplovane sa gasnom hromatografijom",
pages = "364-357",
number = "3",
volume = "66",
doi = "10.2298/HEMIND111114108A"
}

Antić, V., Antić, M., Kronimus, A.,& Schwarzbauer, J.. (2012). Quantitative determination of poly(vinylpyrrolidone) by 'On-line' pyrolysis coupled with gas chromatography. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 66(3), 357-364.
https://doi.org/10.2298/HEMIND111114108A

Antić V, Antić M, Kronimus A, Schwarzbauer J. Quantitative determination of poly(vinylpyrrolidone) by 'On-line' pyrolysis coupled with gas chromatography. in Hemijska industrija. 2012;66(3):357-364.
doi:10.2298/HEMIND111114108A .

Antić, Vesna, Antić, Mališa, Kronimus, Alexander, Schwarzbauer, Jan, "Quantitative determination of poly(vinylpyrrolidone) by 'On-line' pyrolysis coupled with gas chromatography" in Hemijska industrija, 66, no. 3 (2012):357-364,
https://doi.org/10.2298/HEMIND111114108A . .

TY  - JOUR
AU  - Ilić, Mila
AU  - Antić, Mališa
AU  - Antić, Vesna
AU  - Schwarzbauer, Jan
AU  - Vrvić, Miroslav M.
AU  - Jovančićević, Branimir
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2691
AB  - Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Panevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Chemistry Letters
T1  - Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation
EP  - 140
IS  - 1
SP  - 133
VL  - 9
DO  - 10.1007/s10311-009-0257-3
ER  - 

@article{
author = "Ilić, Mila and Antić, Mališa and Antić, Vesna and Schwarzbauer, Jan and Vrvić, Miroslav M. and Jovančićević, Branimir",
year = "2011",
abstract = "Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Panevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Chemistry Letters",
title = "Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation",
pages = "140-133",
number = "1",
volume = "9",
doi = "10.1007/s10311-009-0257-3"
}

Ilić, M., Antić, M., Antić, V., Schwarzbauer, J., Vrvić, M. M.,& Jovančićević, B.. (2011). Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. in Environmental Chemistry Letters
Springer Heidelberg, Heidelberg., 9(1), 133-140.
https://doi.org/10.1007/s10311-009-0257-3

Ilić M, Antić M, Antić V, Schwarzbauer J, Vrvić MM, Jovančićević B. Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. in Environmental Chemistry Letters. 2011;9(1):133-140.
doi:10.1007/s10311-009-0257-3 .

Ilić, Mila, Antić, Mališa, Antić, Vesna, Schwarzbauer, Jan, Vrvić, Miroslav M., Jovančićević, Branimir, "Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation" in Environmental Chemistry Letters, 9, no. 1 (2011):133-140,
https://doi.org/10.1007/s10311-009-0257-3 . .

TY  - JOUR
AU  - Antić, Vesna
AU  - Antić, Mališa
AU  - Kronimus, Alexander
AU  - Oing, Katja
AU  - Schwarzbauer, Jan
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2721
AB  - Poly(vinylpyrrolidone) (PVP) is a widely used and environmentally stable synthetic polymer whose occurrence in wastewater is likely. However, an appropriate method for identification and quantification of this polymer in environmental samples is still lacking. Hence, in this study an analytical method based on continuous-flow off-line pyrolysis-gas chromatography/mass spectrometry (pyrolysis-GC/MS) was developed to identify and quantify PVP in waste and surface water samples. Pyrolysis of commercial PVP, of some personal care products, of spiked water samples as well as of wastewater samples and river water samples was performed in a tube furnace at 500 degrees C under a continuous nitrogen flow. GC/MS was used for identification of specific degradation products, while GC-FID analysis was performed for quantitative determination. The concentration of PVP was calculated on the basis of the main pyrolytic product, N-vinylpyrrolidone (NVP). Very good linear correlation between initial amounts of PVP and released amounts of NVP was obtained and used as external calibration. The detected level of PVP in investigated personal care products was 6.4 (hair gel), 0.8 (laundry detergent) and 1.4%(tablets for throat disinfection). Spiked water samples, wastewater samples and river water samples were pre-extracted with hexane and diethyl ether prior to pyrolysis. It was found that the recovery of spiked samples was 94.6 +/- 1.6% which indicated that pre-extraction did not provoke significant loss of polymer. Finally, application of the developed method on real environmental samples (river water) revealed that PVP was present in effluents from wastewater treatment plants in concentrations between 0.9 mg/L and 7 mg/L, as well as in river water affected by municipal sewage emissions with concentrations around 0.1 mg/L.
PB  - Elsevier, Amsterdam
T2  - Journal of Analytical and Applied Pyrolysis
T1  - Quantitative determination of poly(vinylpyrrolidone) by continuous-flow off-line pyrolysis-GC/MS
EP  - 99
IS  - 2
SP  - 93
VL  - 90
DO  - 10.1016/j.jaap.2010.10.011
ER  - 

@article{
author = "Antić, Vesna and Antić, Mališa and Kronimus, Alexander and Oing, Katja and Schwarzbauer, Jan",
year = "2011",
abstract = "Poly(vinylpyrrolidone) (PVP) is a widely used and environmentally stable synthetic polymer whose occurrence in wastewater is likely. However, an appropriate method for identification and quantification of this polymer in environmental samples is still lacking. Hence, in this study an analytical method based on continuous-flow off-line pyrolysis-gas chromatography/mass spectrometry (pyrolysis-GC/MS) was developed to identify and quantify PVP in waste and surface water samples. Pyrolysis of commercial PVP, of some personal care products, of spiked water samples as well as of wastewater samples and river water samples was performed in a tube furnace at 500 degrees C under a continuous nitrogen flow. GC/MS was used for identification of specific degradation products, while GC-FID analysis was performed for quantitative determination. The concentration of PVP was calculated on the basis of the main pyrolytic product, N-vinylpyrrolidone (NVP). Very good linear correlation between initial amounts of PVP and released amounts of NVP was obtained and used as external calibration. The detected level of PVP in investigated personal care products was 6.4 (hair gel), 0.8 (laundry detergent) and 1.4%(tablets for throat disinfection). Spiked water samples, wastewater samples and river water samples were pre-extracted with hexane and diethyl ether prior to pyrolysis. It was found that the recovery of spiked samples was 94.6 +/- 1.6% which indicated that pre-extraction did not provoke significant loss of polymer. Finally, application of the developed method on real environmental samples (river water) revealed that PVP was present in effluents from wastewater treatment plants in concentrations between 0.9 mg/L and 7 mg/L, as well as in river water affected by municipal sewage emissions with concentrations around 0.1 mg/L.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Analytical and Applied Pyrolysis",
title = "Quantitative determination of poly(vinylpyrrolidone) by continuous-flow off-line pyrolysis-GC/MS",
pages = "99-93",
number = "2",
volume = "90",
doi = "10.1016/j.jaap.2010.10.011"
}

Antić, V., Antić, M., Kronimus, A., Oing, K.,& Schwarzbauer, J.. (2011). Quantitative determination of poly(vinylpyrrolidone) by continuous-flow off-line pyrolysis-GC/MS. in Journal of Analytical and Applied Pyrolysis
Elsevier, Amsterdam., 90(2), 93-99.
https://doi.org/10.1016/j.jaap.2010.10.011

Antić V, Antić M, Kronimus A, Oing K, Schwarzbauer J. Quantitative determination of poly(vinylpyrrolidone) by continuous-flow off-line pyrolysis-GC/MS. in Journal of Analytical and Applied Pyrolysis. 2011;90(2):93-99.
doi:10.1016/j.jaap.2010.10.011 .

Antić, Vesna, Antić, Mališa, Kronimus, Alexander, Oing, Katja, Schwarzbauer, Jan, "Quantitative determination of poly(vinylpyrrolidone) by continuous-flow off-line pyrolysis-GC/MS" in Journal of Analytical and Applied Pyrolysis, 90, no. 2 (2011):93-99,
https://doi.org/10.1016/j.jaap.2010.10.011 . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Vrvić, Miroslav M.
AU  - Ilić, M.
AU  - Novaković, M.
AU  - Saheed, R.M.
AU  - Schwarzbauer, Jan
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1808
AB  - The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P-1-P-12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P-8-P-12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Transformation of a petroleum pollutant during soil bioremediation experiments
EP  - 583
IS  - 5
SP  - 577
VL  - 73
DO  - 10.2298/JSC0805577J
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Vrvić, Miroslav M. and Ilić, M. and Novaković, M. and Saheed, R.M. and Schwarzbauer, Jan",
year = "2008",
abstract = "The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P-1-P-12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P-8-P-12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Transformation of a petroleum pollutant during soil bioremediation experiments",
pages = "583-577",
number = "5",
volume = "73",
doi = "10.2298/JSC0805577J"
}

Jovančićević, B., Antić, M., Vrvić, M. M., Ilić, M., Novaković, M., Saheed, R.M.,& Schwarzbauer, J.. (2008). Transformation of a petroleum pollutant during soil bioremediation experiments. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 73(5), 577-583.
https://doi.org/10.2298/JSC0805577J

Jovančićević B, Antić M, Vrvić MM, Ilić M, Novaković M, Saheed R, Schwarzbauer J. Transformation of a petroleum pollutant during soil bioremediation experiments. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2008;73(5):577-583.
doi:10.2298/JSC0805577J .

Jovančićević, Branimir, Antić, Mališa, Vrvić, Miroslav M., Ilić, M., Novaković, M., Saheed, R.M., Schwarzbauer, Jan, "Transformation of a petroleum pollutant during soil bioremediation experiments" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 73, no. 5 (2008):577-583,
https://doi.org/10.2298/JSC0805577J . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Pavlović, I.
AU  - Vrvić, Miroslav M.
AU  - Beskoski, Vladimir
AU  - Kronimus, Alexander
AU  - Schwarzbauer, Jan
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1632
AB  - This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pančevo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P1-P12 samples). Organic substance was extracted by Soxhlet's method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P1. Pristane and phytane occurred in sample P8, and in even higher quantities in the final sample P12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P1 the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P8 and P12 because of the reduced concentration. The observed changes in the alkane fractions' compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.
T2  - Water, Air, and Soil Pollution
T1  - Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments
EP  - 307
IS  - 1-4
SP  - 299
VL  - 190
DO  - 10.1007/s11270-007-9601-z
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Pavlović, I. and Vrvić, Miroslav M. and Beskoski, Vladimir and Kronimus, Alexander and Schwarzbauer, Jan",
year = "2008",
abstract = "This paper presents transformations of saturated hydrocarbons of petroleum type pollutants during ex situ bioremediation of soil on the pilot heap (halde), during a period of 6 months, within the grounds of Petroleum Refinery Pančevo (Serbia). Samples for analysis were taken in time intervals of 2 weeks (P1-P12 samples). Organic substance was extracted by Soxhlet's method and quantified. Isoprenoid aliphatics, in particular pristane and phytane, and polycyclic aliphatics of sterane and triterpane types in saturated hydrocarbon fractions were analysed by GC-MS (SIM method). Significant amounts of n-alkanes have not been detected. The MS-chromatogram revealed only marginal amounts of pristane and phytane in sample P1. Pristane and phytane occurred in sample P8, and in even higher quantities in the final sample P12. The proceeding bioremediation process was accompanied by the decrease of the relative amounts of pentacyclic terpanes of hopane type, compared to tri- and tetracyclic terpanes. In the initial sample P1 the distribution of steranes and hopanes follows a pattern, which is characteristic for crude oils. However, their identification by SIM method was not possible in samples P8 and P12 because of the reduced concentration. The observed changes in the alkane fractions' compositions may be considered as atypical, referring to the fact that during oil biodegradation under natural conditions, decomposition of isoprenoids occurs much easier and faster than decomposition of polycyclic alkanes of tri-, tetra- and pentacyclic terpane, sterane and diasterane types, after the decomposition of n-alkanes has been almost completed.",
journal = "Water, Air, and Soil Pollution",
title = "Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments",
pages = "307-299",
number = "1-4",
volume = "190",
doi = "10.1007/s11270-007-9601-z"
}

Jovančićević, B., Antić, M., Pavlović, I., Vrvić, M. M., Beskoski, V., Kronimus, A.,& Schwarzbauer, J.. (2008). Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments. in Water, Air, and Soil Pollution, 190(1-4), 299-307.
https://doi.org/10.1007/s11270-007-9601-z

Jovančićević B, Antić M, Pavlović I, Vrvić MM, Beskoski V, Kronimus A, Schwarzbauer J. Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments. in Water, Air, and Soil Pollution. 2008;190(1-4):299-307.
doi:10.1007/s11270-007-9601-z .

Jovančićević, Branimir, Antić, Mališa, Pavlović, I., Vrvić, Miroslav M., Beskoski, Vladimir, Kronimus, Alexander, Schwarzbauer, Jan, "Transformation of petroleum saturated hydrocarbons during soil bioremediation experiments" in Water, Air, and Soil Pollution, 190, no. 1-4 (2008):299-307,
https://doi.org/10.1007/s11270-007-9601-z . .

TY  - JOUR
AU  - Antić, Mališa
AU  - Jovančićević, Branimir
AU  - Ilić, Mila
AU  - Vrvić, Miroslav M.
AU  - Schwarzbauer, Jan
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1351
AB  - BACKGROUND: , Aims and Scope. It is well known that the composition of petroleum or some of its processing products changes in the environment mostly under the influence of microorganisms. A series of experiments was conducted in order to define the optimum conditions for an efficient biodegradation of petroleum pollutant, or bioremediation of different segments of the environment. The aim of these investigations was to show to what extent the hydrocarbons of a petroleum pollutant are degraded by microbial cultures which were isolated as dominant microorganisms from a surface water of a wastewater canal of an oil refinery and a nitrogen plant. Biodegradation experiments were conducted on one paraffinic, and one naphthenic type of petroleum during a three month period under aerobic conditions, varying the following parameters: Inorganic (Kp) or an organic medium (Bh) with or without exposition to light. Methods. Microorganisms were analyzed in a surface water sample from a canal (Pancevo, Serbia), into which wastewater from an oil refinery and a nitrogen plant is released. The consortia of microorganisms were isolated from the water sample (most abundant species: Phormidium foveolarum - filamentous Cyanobacteria, blue-green algae and Achanthes minutissima, diatoms, algae). The simulation experiments of biodegradation were conducted with the biomass suspension and crude oils Sirakovo (Sir, paraffinic type) and Velebit (Ve, naphthenic type). After a three month period, organic substance was extracted by means of chloroform. In the extracts, the content of saturated hydrocarbons, aromatic hydrocarbons, alcohols and fatty acids was determined (the group composition). n-Alkanes and isoprenoid aliphatic alkanes, pristane and phytane, in the aliphatic fractions, were analyzed using gas chromatography (GC). Total isoprenoid aliphatic alkanes and polycyclic alkanes of sterane and triterpane types were analyzed by GC-MS. Results and Discussion. Paraffinic type petroleums have a significant loss of saturated hydrocarbons. For naphthenic type petroleum, such a trend has not been observed. The most intensive degradation of n-alkanes and isoprenoid aliphatic alkanes (in paraffinic oil) and isoprenoids (in naphthenic oil) was observed using the inorganic medium Kp in the light; the microbial conversion is somewhat lower with Kp in the dark; with organic medium Bh in the light the degradation is of low intensity; with the same medium in the dark the degradation is hardly to be seen. Steranes and triterpanes were not affected by microbial degradation under the conditions used in our experiments. Obviously, the petroleum biodegradation was restricted to the acyclic aliphatics (n-alkanes and isoprenoids). Conclusion. Phormidium foveolarum (filamentous Cyanobacteria - blue-green algae) and Achanthes minutissima (diatoms, algae), microbial cultures isolated as dominant algae from a surface water in a wastewater canal of an oil refinery and a nitrogen plant, have degradable effects dominantly involving petroleum hydocarbons. Petroleum microbiological degradation is more intensive when inorganic medium (in the light) is applied. Having in mind that the inorganic pollutants have been released into the canal as well, this medium reflects more the natural environmental conditions. Polycyclic alkanes of sterane and triterpane type, in spite of the fact that these compounds could be degraded, have remained unchanged regarding abundance and distribution. Since this is the case even for naphthenic type petroleum (which is depleted in n-alkanes), it can be concluded that the biodegradation of petroleum type pollutants, under natural conditions, will be restrained to the n-alkane and isoprenoid degradation. Recommendation and Outlook. Performed experiments and simulations of petroleum microbiological degradation may serve for the prediction of the fate of petroleum type pollutants, as well as for definition of conditions for bioremediation of some environmental segments.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Petroleum pollutant degradation by surface water microorganisms
EP  - 327
IS  - 5
SP  - 320
VL  - 13
DO  - 10.1065/espr2006.03.296
ER  - 

@article{
author = "Antić, Mališa and Jovančićević, Branimir and Ilić, Mila and Vrvić, Miroslav M. and Schwarzbauer, Jan",
year = "2006",
abstract = "BACKGROUND: , Aims and Scope. It is well known that the composition of petroleum or some of its processing products changes in the environment mostly under the influence of microorganisms. A series of experiments was conducted in order to define the optimum conditions for an efficient biodegradation of petroleum pollutant, or bioremediation of different segments of the environment. The aim of these investigations was to show to what extent the hydrocarbons of a petroleum pollutant are degraded by microbial cultures which were isolated as dominant microorganisms from a surface water of a wastewater canal of an oil refinery and a nitrogen plant. Biodegradation experiments were conducted on one paraffinic, and one naphthenic type of petroleum during a three month period under aerobic conditions, varying the following parameters: Inorganic (Kp) or an organic medium (Bh) with or without exposition to light. Methods. Microorganisms were analyzed in a surface water sample from a canal (Pancevo, Serbia), into which wastewater from an oil refinery and a nitrogen plant is released. The consortia of microorganisms were isolated from the water sample (most abundant species: Phormidium foveolarum - filamentous Cyanobacteria, blue-green algae and Achanthes minutissima, diatoms, algae). The simulation experiments of biodegradation were conducted with the biomass suspension and crude oils Sirakovo (Sir, paraffinic type) and Velebit (Ve, naphthenic type). After a three month period, organic substance was extracted by means of chloroform. In the extracts, the content of saturated hydrocarbons, aromatic hydrocarbons, alcohols and fatty acids was determined (the group composition). n-Alkanes and isoprenoid aliphatic alkanes, pristane and phytane, in the aliphatic fractions, were analyzed using gas chromatography (GC). Total isoprenoid aliphatic alkanes and polycyclic alkanes of sterane and triterpane types were analyzed by GC-MS. Results and Discussion. Paraffinic type petroleums have a significant loss of saturated hydrocarbons. For naphthenic type petroleum, such a trend has not been observed. The most intensive degradation of n-alkanes and isoprenoid aliphatic alkanes (in paraffinic oil) and isoprenoids (in naphthenic oil) was observed using the inorganic medium Kp in the light; the microbial conversion is somewhat lower with Kp in the dark; with organic medium Bh in the light the degradation is of low intensity; with the same medium in the dark the degradation is hardly to be seen. Steranes and triterpanes were not affected by microbial degradation under the conditions used in our experiments. Obviously, the petroleum biodegradation was restricted to the acyclic aliphatics (n-alkanes and isoprenoids). Conclusion. Phormidium foveolarum (filamentous Cyanobacteria - blue-green algae) and Achanthes minutissima (diatoms, algae), microbial cultures isolated as dominant algae from a surface water in a wastewater canal of an oil refinery and a nitrogen plant, have degradable effects dominantly involving petroleum hydocarbons. Petroleum microbiological degradation is more intensive when inorganic medium (in the light) is applied. Having in mind that the inorganic pollutants have been released into the canal as well, this medium reflects more the natural environmental conditions. Polycyclic alkanes of sterane and triterpane type, in spite of the fact that these compounds could be degraded, have remained unchanged regarding abundance and distribution. Since this is the case even for naphthenic type petroleum (which is depleted in n-alkanes), it can be concluded that the biodegradation of petroleum type pollutants, under natural conditions, will be restrained to the n-alkane and isoprenoid degradation. Recommendation and Outlook. Performed experiments and simulations of petroleum microbiological degradation may serve for the prediction of the fate of petroleum type pollutants, as well as for definition of conditions for bioremediation of some environmental segments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Petroleum pollutant degradation by surface water microorganisms",
pages = "327-320",
number = "5",
volume = "13",
doi = "10.1065/espr2006.03.296"
}

Antić, M., Jovančićević, B., Ilić, M., Vrvić, M. M.,& Schwarzbauer, J.. (2006). Petroleum pollutant degradation by surface water microorganisms. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 13(5), 320-327.
https://doi.org/10.1065/espr2006.03.296

Antić M, Jovančićević B, Ilić M, Vrvić MM, Schwarzbauer J. Petroleum pollutant degradation by surface water microorganisms. in Environmental Science and Pollution Research. 2006;13(5):320-327.
doi:10.1065/espr2006.03.296 .

Antić, Mališa, Jovančićević, Branimir, Ilić, Mila, Vrvić, Miroslav M., Schwarzbauer, Jan, "Petroleum pollutant degradation by surface water microorganisms" in Environmental Science and Pollution Research, 13, no. 5 (2006):320-327,
https://doi.org/10.1065/espr2006.03.296 . .

TY  - JOUR
AU  - Jovančićević, Branimir
AU  - Antić, Mališa
AU  - Solević, TM
AU  - Vrvić, Miroslav M.
AU  - Kronimus, Alexander
AU  - Schwarzbauer, Jan
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1010
AB  - BACKGROUND: , Aims and Scope. In oil spill investigations, one of the most important steps is a proper choice of approaches that imply an investigation of samples taken from different sedimentary environments, samples of oil contaminants taken in different periods of time and samples taken at different distances from the oil spill. In all these cases, conclusion on the influence of the environment, microorganisms or migration on the oil contaminants' composition can be drawn from the comparison of chemical compositions of the investigated contaminants. However, in case of water contaminants, it is very important to define which part of organic matter has been analyzed. Namely, previous investigations showed that there were some differences in chemical composition of the same oil contaminant depending on the intensity of its contact with ground water. The aim of this work is to define more precisely the interactions between oil contaminant and water, i.e. the influence of the intensity of interaction between the oil contaminant and water on its chemical composition. The study was based on a comparison of four fractionated extracts of an oil pollutant, after they had been analyzed in details. Methods. Oil polluted surface water (wastewater canal, Pancevo, Serbia) was investigated. The study was based on a comparison of four extracts of an oil contaminant: extract I (decanted part), and extracts 2, 3 and 4 (extracted by shaking for 1 minute, 5 minutes and 24 hours, respectively). The fractionated extracts were saponified with a solution of KOH in methanol, and neutralized with 10% hydrochloric acid. The products were dissolved in a mixture of dichloromethane and hexane, and individually fractionated by column chromatography on alumina and silica gel (saturated hydrocarbon, aromatic, alcohol and fatty acid fractions). n-Alkanes and isoprenoid aliphatic alkanes, polycyclic alkanes of sterane and triterpane types, alcohols and fatty acids were analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). delta C-13(PDB) values of individual n-alkanes in the aliphatic fractions were determined using gas chromatography-isotope ratio monitoring-mass spectrometry (GC-irmMS). Results and discussion. Extracts 1 and 2 are characterized by uniform distribution of n-alkanes, whereas extract 3 is characterized by an even-numbered members dominating the odd-ones, and extract 4 showed a bimodal distribution. Extract I is characterized by the least negative delta C-13(PDB) values of C-19-C-26 n-alkanes. Sterane and triterpane analysis confirmed that all extracts originated from the same oil contaminant. n-Fatty acids, C-19-C-24, in all extracts are very low, being somewhat higher in extract 4. Even-numbered n-alcohols, C-12-C-16, were identified in the highest concentration in extract 3. It was assumed that algae were responsible for the composition of extract 3. Furthermore, a possible reason for higher concentrations of C-19-C-26 n-alkanes and C-19-C-24 fatty acids in extract 4 is the formation of inclusion compounds with colloidal micelles formed between the oil contaminant's NSO-compounds and water. Conclusion. It was undoubtedly confirmed that there were specific differences in the compositions of the different extracts depending on the intensity of the interaction between the oil contaminant and the surface water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Investigation of interactions between surface water and petroleum-type pollutants
EP  - 212
IS  - 4
SP  - 205
VL  - 12
DO  - 10.1065/espr2004.12.229
ER  - 

@article{
author = "Jovančićević, Branimir and Antić, Mališa and Solević, TM and Vrvić, Miroslav M. and Kronimus, Alexander and Schwarzbauer, Jan",
year = "2005",
abstract = "BACKGROUND: , Aims and Scope. In oil spill investigations, one of the most important steps is a proper choice of approaches that imply an investigation of samples taken from different sedimentary environments, samples of oil contaminants taken in different periods of time and samples taken at different distances from the oil spill. In all these cases, conclusion on the influence of the environment, microorganisms or migration on the oil contaminants' composition can be drawn from the comparison of chemical compositions of the investigated contaminants. However, in case of water contaminants, it is very important to define which part of organic matter has been analyzed. Namely, previous investigations showed that there were some differences in chemical composition of the same oil contaminant depending on the intensity of its contact with ground water. The aim of this work is to define more precisely the interactions between oil contaminant and water, i.e. the influence of the intensity of interaction between the oil contaminant and water on its chemical composition. The study was based on a comparison of four fractionated extracts of an oil pollutant, after they had been analyzed in details. Methods. Oil polluted surface water (wastewater canal, Pancevo, Serbia) was investigated. The study was based on a comparison of four extracts of an oil contaminant: extract I (decanted part), and extracts 2, 3 and 4 (extracted by shaking for 1 minute, 5 minutes and 24 hours, respectively). The fractionated extracts were saponified with a solution of KOH in methanol, and neutralized with 10% hydrochloric acid. The products were dissolved in a mixture of dichloromethane and hexane, and individually fractionated by column chromatography on alumina and silica gel (saturated hydrocarbon, aromatic, alcohol and fatty acid fractions). n-Alkanes and isoprenoid aliphatic alkanes, polycyclic alkanes of sterane and triterpane types, alcohols and fatty acids were analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). delta C-13(PDB) values of individual n-alkanes in the aliphatic fractions were determined using gas chromatography-isotope ratio monitoring-mass spectrometry (GC-irmMS). Results and discussion. Extracts 1 and 2 are characterized by uniform distribution of n-alkanes, whereas extract 3 is characterized by an even-numbered members dominating the odd-ones, and extract 4 showed a bimodal distribution. Extract I is characterized by the least negative delta C-13(PDB) values of C-19-C-26 n-alkanes. Sterane and triterpane analysis confirmed that all extracts originated from the same oil contaminant. n-Fatty acids, C-19-C-24, in all extracts are very low, being somewhat higher in extract 4. Even-numbered n-alcohols, C-12-C-16, were identified in the highest concentration in extract 3. It was assumed that algae were responsible for the composition of extract 3. Furthermore, a possible reason for higher concentrations of C-19-C-26 n-alkanes and C-19-C-24 fatty acids in extract 4 is the formation of inclusion compounds with colloidal micelles formed between the oil contaminant's NSO-compounds and water. Conclusion. It was undoubtedly confirmed that there were specific differences in the compositions of the different extracts depending on the intensity of the interaction between the oil contaminant and the surface water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water. Recommendation and Outlook. When comparing the composition of oil contaminants from different water samples (regardless of the ultimate investigation goal) it is necessary to compare the extracts isolated under the same conditions, in other words, extracts that were in the same or very similar interaction with water.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Investigation of interactions between surface water and petroleum-type pollutants",
pages = "212-205",
number = "4",
volume = "12",
doi = "10.1065/espr2004.12.229"
}

Jovančićević, B., Antić, M., Solević, T., Vrvić, M. M., Kronimus, A.,& Schwarzbauer, J.. (2005). Investigation of interactions between surface water and petroleum-type pollutants. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 12(4), 205-212.
https://doi.org/10.1065/espr2004.12.229

Jovančićević B, Antić M, Solević T, Vrvić MM, Kronimus A, Schwarzbauer J. Investigation of interactions between surface water and petroleum-type pollutants. in Environmental Science and Pollution Research. 2005;12(4):205-212.
doi:10.1065/espr2004.12.229 .

Jovančićević, Branimir, Antić, Mališa, Solević, TM, Vrvić, Miroslav M., Kronimus, Alexander, Schwarzbauer, Jan, "Investigation of interactions between surface water and petroleum-type pollutants" in Environmental Science and Pollution Research, 12, no. 4 (2005):205-212,
https://doi.org/10.1065/espr2004.12.229 . .