TY  - JOUR
AU  - Stojanova, Monika
AU  - Pantic, Milena
AU  - Klaus, Anita
AU  - Mihajlovic, Dragana
AU  - Miletic, Dunja
AU  - Sobajic, Sladjana
AU  - Stojanova, Marina Todor
AU  - Niksic, Miomir
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6334
AB  - The aim of this research was to produce a lyophilised water and ethanol extract from Fuscoporia torulosa mushroom, to determine the antimicrobial and antioxidant potential of the extracts and in industrial conditions to produce a new, functional product – Bio Soups, enriched with lyophilised extracts derived from fruiting body of F. torulosa mushroom, at the same time without use of synthetic additives. The application of the lyophilised extracts in the industrial production of dehydrated soups showed positive results. Such soups are characterised with higher (P < 0.05) antimicrobial and antioxidant activity, compared to conventional soups, while it has been proven constant biological potential and stability up to the 90th day of production. This is the first dehydrated soup enriched with lyophilised extract of medicinal mushroom, since its production does not require special conditions, and it possesses exceptional functional properties, this product could have a many times greater market potential compared to conventional soups. © 2023 Institute of Food, Science and Technology (IFSTTF).
T2  - International Journal of Food Science and Technology
T2  - International Journal of Food Science and Technology
T1  - Bio soups – new functional dehydrated soups enriched with lyophilised Fuscoporia torulosa extracts
DO  - 10.1111/ijfs.16462
ER  - 

@article{
author = "Stojanova, Monika and Pantic, Milena and Klaus, Anita and Mihajlovic, Dragana and Miletic, Dunja and Sobajic, Sladjana and Stojanova, Marina Todor and Niksic, Miomir",
year = "2023",
abstract = "The aim of this research was to produce a lyophilised water and ethanol extract from Fuscoporia torulosa mushroom, to determine the antimicrobial and antioxidant potential of the extracts and in industrial conditions to produce a new, functional product – Bio Soups, enriched with lyophilised extracts derived from fruiting body of F. torulosa mushroom, at the same time without use of synthetic additives. The application of the lyophilised extracts in the industrial production of dehydrated soups showed positive results. Such soups are characterised with higher (P < 0.05) antimicrobial and antioxidant activity, compared to conventional soups, while it has been proven constant biological potential and stability up to the 90th day of production. This is the first dehydrated soup enriched with lyophilised extract of medicinal mushroom, since its production does not require special conditions, and it possesses exceptional functional properties, this product could have a many times greater market potential compared to conventional soups. © 2023 Institute of Food, Science and Technology (IFSTTF).",
journal = "International Journal of Food Science and Technology, International Journal of Food Science and Technology",
title = "Bio soups – new functional dehydrated soups enriched with lyophilised Fuscoporia torulosa extracts",
doi = "10.1111/ijfs.16462"
}

Stojanova, M., Pantic, M., Klaus, A., Mihajlovic, D., Miletic, D., Sobajic, S., Stojanova, M. T.,& Niksic, M.. (2023). Bio soups – new functional dehydrated soups enriched with lyophilised Fuscoporia torulosa extracts. in International Journal of Food Science and Technology.
https://doi.org/10.1111/ijfs.16462

Stojanova M, Pantic M, Klaus A, Mihajlovic D, Miletic D, Sobajic S, Stojanova MT, Niksic M. Bio soups – new functional dehydrated soups enriched with lyophilised Fuscoporia torulosa extracts. in International Journal of Food Science and Technology. 2023;.
doi:10.1111/ijfs.16462 .

Stojanova, Monika, Pantic, Milena, Klaus, Anita, Mihajlovic, Dragana, Miletic, Dunja, Sobajic, Sladjana, Stojanova, Marina Todor, Niksic, Miomir, "Bio soups – new functional dehydrated soups enriched with lyophilised Fuscoporia torulosa extracts" in International Journal of Food Science and Technology (2023),
https://doi.org/10.1111/ijfs.16462 . .

Miletić, D., Sknepnek, A., Hadnađev, M., Dapčević Hadnađev, T., Pantić, M., Nedović, V.,& Lević, S.. (2023). Novi pekarski proizvod - hleb obogaćen selenom dobijen dodatkom selenom obogaćene biomase Coriolus versicolor gljive. .
https://hdl.handle.net/21.15107/rcub_agrospace_6496

Miletić D, Sknepnek A, Hadnađev M, Dapčević Hadnađev T, Pantić M, Nedović V, Lević S. Novi pekarski proizvod - hleb obogaćen selenom dobijen dodatkom selenom obogaćene biomase Coriolus versicolor gljive. 2023;.
https://hdl.handle.net/21.15107/rcub_agrospace_6496 .

Miletić, Dunja, Sknepnek, Aleksandra, Hadnađev, Miroslav, Dapčević Hadnađev, Tamara, Pantić, Milena, Nedović, Viktor, Lević, Steva, "Novi pekarski proizvod - hleb obogaćen selenom dobijen dodatkom selenom obogaćene biomase Coriolus versicolor gljive" (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6496 .

TY  - JOUR
AU  - Salević-Jelić, Ana
AU  - Lević, Steva
AU  - Stojanović, Dušica
AU  - Jeremić, Sanja
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Ignjatović, Ivana Sredović
AU  - Uskoković, Petar
AU  - Nedović, Viktor
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6260
AB  - This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days). © 2023 Elsevier Ltd
T2  - Food Packaging and Shelf Life
T2  - Food Packaging and Shelf Life
T1  - Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization
VL  - 35
DO  - 10.1016/j.fpsl.2023.101027
ER  - 

@article{
author = "Salević-Jelić, Ana and Lević, Steva and Stojanović, Dušica and Jeremić, Sanja and Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Ignjatović, Ivana Sredović and Uskoković, Petar and Nedović, Viktor",
year = "2023",
abstract = "This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days). © 2023 Elsevier Ltd",
journal = "Food Packaging and Shelf Life, Food Packaging and Shelf Life",
title = "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization",
volume = "35",
doi = "10.1016/j.fpsl.2023.101027"
}

Salević-Jelić, A., Lević, S., Stojanović, D., Jeremić, S., Miletić, D., Pantić, M., Pavlović, V., Ignjatović, I. S., Uskoković, P.,& Nedović, V.. (2023). Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life, 35.
https://doi.org/10.1016/j.fpsl.2023.101027

Salević-Jelić A, Lević S, Stojanović D, Jeremić S, Miletić D, Pantić M, Pavlović V, Ignjatović IS, Uskoković P, Nedović V. Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life. 2023;35.
doi:10.1016/j.fpsl.2023.101027 .

Salević-Jelić, Ana, Lević, Steva, Stojanović, Dušica, Jeremić, Sanja, Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Ignjatović, Ivana Sredović, Uskoković, Petar, Nedović, Viktor, "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization" in Food Packaging and Shelf Life, 35 (2023),
https://doi.org/10.1016/j.fpsl.2023.101027 . .

TY  - GEN
AU  - Pantić, Milena
AU  - Miletić, Dunja
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6619
AB  - Selenium (Se) is an essential micronutrient which bioavailability and toxicity depends on its form and
concentration that is taken into the body. Se uptake from organic sources goes up to 90-95%, while
from inorganic it’s up to 10%. In addition, toxicity of organic Se compounds is low. In the last decades,
the interest in functional foods and natural dietary supplements consummation has been growing.
Since the mushroom market also constantly grows, bioactive compounds, isolated from higher fungi
grown on fortified substrates, are their promising source. During last 15 years of research, the biological
activity of Se-enriched macromycetes has been proven through in vitro tests by our group. The
research was conducted on selenium enriched mushroom species (Pleurotus spp, Lentinus edodes,
Coriolus versicolor and Agaricus bisporus) obtained using solid state and submerged cultivation techniques,
and different sources of selenium for the growing substrate supplementation.
Chemical composition analysis revealed that, mostly, Se-enriched extracts and polysaccharides obtained
from mycelia and fruiting bodies had higher content of glucans, total proteins and phenolic
compounds compared to non-enriched samples. Also, a significant percentage of total accumulated
Se was incorporated in the form of L-selenomethionine. Mushrooms enriched with Se from an organic
source (selenourea, Se-yeast) showed significantly different Se-accumulation efficiency, biomass yield,
content of the L-selenomethionine, Se content and different biological activity, compared to the mushroom
samples enriched with inorganic salts (sodium selenate and sodium selenite).
The ability of some mushroom species to grow and accumulate selenium also varied on used cultivation
technique and growth stage. In vitro test of the fresh fruiting bodies indicated the positive influence
of Se from Se-modified zeolites on the activity of enzymes of primary antioxidant protection - superoxide
dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT).Antioxidant activity of selenium enriched extracts depended on the Se concentration, form of the Se
used for the supplementation, mushroom strain and type of the mushroom extract. An increase in the
activity was noted with increasing concentration of Se in all tested samples.
Furthermore, antibacterial activity against food-borne pathogens and non-toxicity to moderate toxicity
of extracts, with high content of Se, against normal MRC-5 cell line was proven. According to results,
Se-enriched mushrooms in the form of fruiting bodies, mycelia, extracts or polysaccharide extracts
could be a promising non-toxic dietary supplements or functional food/additives with prolonged shelf
life and improved bioactivity.
T2  - 11th International Medicinal Mushroom Conference (IMMC11)
T1  - Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry
EP  - 140
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6619
ER  - 

@misc{
author = "Pantić, Milena and Miletić, Dunja and Matijašević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2022",
abstract = "Selenium (Se) is an essential micronutrient which bioavailability and toxicity depends on its form and
concentration that is taken into the body. Se uptake from organic sources goes up to 90-95%, while
from inorganic it’s up to 10%. In addition, toxicity of organic Se compounds is low. In the last decades,
the interest in functional foods and natural dietary supplements consummation has been growing.
Since the mushroom market also constantly grows, bioactive compounds, isolated from higher fungi
grown on fortified substrates, are their promising source. During last 15 years of research, the biological
activity of Se-enriched macromycetes has been proven through in vitro tests by our group. The
research was conducted on selenium enriched mushroom species (Pleurotus spp, Lentinus edodes,
Coriolus versicolor and Agaricus bisporus) obtained using solid state and submerged cultivation techniques,
and different sources of selenium for the growing substrate supplementation.
Chemical composition analysis revealed that, mostly, Se-enriched extracts and polysaccharides obtained
from mycelia and fruiting bodies had higher content of glucans, total proteins and phenolic
compounds compared to non-enriched samples. Also, a significant percentage of total accumulated
Se was incorporated in the form of L-selenomethionine. Mushrooms enriched with Se from an organic
source (selenourea, Se-yeast) showed significantly different Se-accumulation efficiency, biomass yield,
content of the L-selenomethionine, Se content and different biological activity, compared to the mushroom
samples enriched with inorganic salts (sodium selenate and sodium selenite).
The ability of some mushroom species to grow and accumulate selenium also varied on used cultivation
technique and growth stage. In vitro test of the fresh fruiting bodies indicated the positive influence
of Se from Se-modified zeolites on the activity of enzymes of primary antioxidant protection - superoxide
dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT).Antioxidant activity of selenium enriched extracts depended on the Se concentration, form of the Se
used for the supplementation, mushroom strain and type of the mushroom extract. An increase in the
activity was noted with increasing concentration of Se in all tested samples.
Furthermore, antibacterial activity against food-borne pathogens and non-toxicity to moderate toxicity
of extracts, with high content of Se, against normal MRC-5 cell line was proven. According to results,
Se-enriched mushrooms in the form of fruiting bodies, mycelia, extracts or polysaccharide extracts
could be a promising non-toxic dietary supplements or functional food/additives with prolonged shelf
life and improved bioactivity.",
journal = "11th International Medicinal Mushroom Conference (IMMC11)",
title = "Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry",
pages = "140-139",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6619"
}

Pantić, M., Miletić, D., Matijašević, D., Sknepnek, A.,& Nikšić, M.. (2022). Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry. in 11th International Medicinal Mushroom Conference (IMMC11), 139-140.
https://hdl.handle.net/21.15107/rcub_agrospace_6619

Pantić M, Miletić D, Matijašević D, Sknepnek A, Nikšić M. Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry. in 11th International Medicinal Mushroom Conference (IMMC11). 2022;:139-140.
https://hdl.handle.net/21.15107/rcub_agrospace_6619 .

Pantić, Milena, Miletić, Dunja, Matijašević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry" in 11th International Medicinal Mushroom Conference (IMMC11) (2022):139-140,
https://hdl.handle.net/21.15107/rcub_agrospace_6619 .

TY  - CONF
AU  - Sknepnek, Aleksandra
AU  - Miletić, Dunja
AU  - Mrkonjić, Živan
AU  - Zeković, Zoran
AU  - Nedović, Viktor
AU  - Pavlić, Branimir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6832
AB  - Antimicrobial resistance to antibiotics is growing, affecting increased burden of the health
system and higher incidence of human fatal outcomes. Therefore, the research of novel
antimicrobial substances is of crucial importance. Wild thyme (Thymus serpyllum L.) extracts
were obtained using different conditions of green and environmentally-friendly supercritical
fluid extraction. Sample SFE-TSŽ2 was prepared at 350 bar and 50 °C, while SFE-TSŽ7 was
extracted at 100 bar and 40 °C. Broth microdilution method was applied to determine how
different extraction conditions reflect on sample antimicrobial activity against pathogenic
microorganisms. Two Gram positive strains, Staphylococcus aureus ATCC 25923 and
methicillin-resistant Staphylococcus aureus (MRSA), stood out as the most sensitive to both
samples (minimal inhibitory concentration, MIC value below 0.02 mg/mL). Bactericidal effect
of the sample SFE-TSŽ2 was found for all Gram-positive strains, with minimal bactericidal
concentrations (MBC) lower than for SFE-TSŽ7, except for Staphylococcus aureus (MRSA)
when the same effect of both extracts was found. Significant difference between samples was
observed in inhibitory activity against Gram-negative bacterial strains, revealing higher
efficiency of SFE-TSŽ2 compared to SFE-TSŽ7. The most sensitive to both samples were
Proteus hauseri ATCC 13315 and Yersinia enterocolitica ATCC 27729 with the same MIC
values (0.83 mg/mL for SFE-TSŽ2 and 2.5 mg/mL for SFE-TSŽ7). SFE-TSŽ2 also expressed
inhibitory and fungicidal effects against Candida albicans ATCC 1231 yeast (MIC 2.5 mg/mL;
minimal fungicidal concentration, MFC, 10 mg/mL), while SFE-TSŽ7 showed no activity.
Obtained results showed that applied extraction conditions affected antimicrobial activities,
most probably due to the differences in chemical compositions.
PB  - University of Novi Sad, Faculty of Technology Novi Sad,Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
C3  - 2nd International Conference on Advanced Production and Processing (ICAPP 2022)
T1  - ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS
SP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6832
ER  - 

@conference{
author = "Sknepnek, Aleksandra and Miletić, Dunja and Mrkonjić, Živan and Zeković, Zoran and Nedović, Viktor and Pavlić, Branimir",
year = "2022",
abstract = "Antimicrobial resistance to antibiotics is growing, affecting increased burden of the health
system and higher incidence of human fatal outcomes. Therefore, the research of novel
antimicrobial substances is of crucial importance. Wild thyme (Thymus serpyllum L.) extracts
were obtained using different conditions of green and environmentally-friendly supercritical
fluid extraction. Sample SFE-TSŽ2 was prepared at 350 bar and 50 °C, while SFE-TSŽ7 was
extracted at 100 bar and 40 °C. Broth microdilution method was applied to determine how
different extraction conditions reflect on sample antimicrobial activity against pathogenic
microorganisms. Two Gram positive strains, Staphylococcus aureus ATCC 25923 and
methicillin-resistant Staphylococcus aureus (MRSA), stood out as the most sensitive to both
samples (minimal inhibitory concentration, MIC value below 0.02 mg/mL). Bactericidal effect
of the sample SFE-TSŽ2 was found for all Gram-positive strains, with minimal bactericidal
concentrations (MBC) lower than for SFE-TSŽ7, except for Staphylococcus aureus (MRSA)
when the same effect of both extracts was found. Significant difference between samples was
observed in inhibitory activity against Gram-negative bacterial strains, revealing higher
efficiency of SFE-TSŽ2 compared to SFE-TSŽ7. The most sensitive to both samples were
Proteus hauseri ATCC 13315 and Yersinia enterocolitica ATCC 27729 with the same MIC
values (0.83 mg/mL for SFE-TSŽ2 and 2.5 mg/mL for SFE-TSŽ7). SFE-TSŽ2 also expressed
inhibitory and fungicidal effects against Candida albicans ATCC 1231 yeast (MIC 2.5 mg/mL;
minimal fungicidal concentration, MFC, 10 mg/mL), while SFE-TSŽ7 showed no activity.
Obtained results showed that applied extraction conditions affected antimicrobial activities,
most probably due to the differences in chemical compositions.",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad,Bulevar cara Lazara 1, 21000 Novi Sad, Serbia",
journal = "2nd International Conference on Advanced Production and Processing (ICAPP 2022)",
title = "ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS",
pages = "170",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6832"
}

Sknepnek, A., Miletić, D., Mrkonjić, Ž., Zeković, Z., Nedović, V.,& Pavlić, B.. (2022). ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS. in 2nd International Conference on Advanced Production and Processing (ICAPP 2022)
University of Novi Sad, Faculty of Technology Novi Sad,Bulevar cara Lazara 1, 21000 Novi Sad, Serbia., 170.
https://hdl.handle.net/21.15107/rcub_agrospace_6832

Sknepnek A, Miletić D, Mrkonjić Ž, Zeković Z, Nedović V, Pavlić B. ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS. in 2nd International Conference on Advanced Production and Processing (ICAPP 2022). 2022;:170.
https://hdl.handle.net/21.15107/rcub_agrospace_6832 .

Sknepnek, Aleksandra, Miletić, Dunja, Mrkonjić, Živan, Zeković, Zoran, Nedović, Viktor, Pavlić, Branimir, "ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS" in 2nd International Conference on Advanced Production and Processing (ICAPP 2022) (2022):170,
https://hdl.handle.net/21.15107/rcub_agrospace_6832 .

TY  - CONF
AU  - Pantic, Milena
AU  - Miletić, Dunja
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6645
AB  - Edible and medicinal mushrooms have been used worldwide due to their nutritional 
value and known pharmaceutical properties, which are related to the physiologically 
beneficial substances in the form of primary and secondary metabolites. Their chemical 
composition and biological activity depend on the medium on which they grow and applied 
supplementation. Numerous studies have shown that mushrooms can absorb selenium (Se) 
during growth on the fortified solid substrate or liquid medium, in a concentration that can 
meet the daily needs of humans. The capacity of some mushroom to absorb Se depends on 
multiple factors, including mushroom strain, composition of the medium, ecological pa rameters as well as on the form and concentration in which this mineral is added to the me dium. It was previously reported that strains Pleurotus sp, Agaricus bisporus, Ganoderma lu cidum, and Cordyceps militaris absorbed high concentrations of Se when sodium selenite or 
selenate were applied. However, higher toxicity of inorganic salts that suppressed mycelium 
growth of Hericium erinaceum, Lentinus edodes, Coriolus versicolor, and Pleurotus spp. com paring to modified zeolites with Se and organic selenitetriglycerides from Selol, Se-yeast, 
and selenourea was proven. Chemical characterization of polysaccharide and methanol ex tracts isolated from fruiting bodies or mycelia culture showed that most of the Se-enriched 
mushrooms had a higher content of total carbohydrates (especially β-glucans), proteins, fla vonoids, and phenols comparing to the non-enriched samples. As a consequence, selenized 
mushrooms showed improved antioxidant and antimicrobial properties. A significant per centage of Se was incorporated not only in the form of L-selenomethionine, but also other 
organic compounds, such as biologically active polysaccharides. It is believed that the syn ergism of Se and bioactive polysaccharides present in fungi could enhance their antitumor 
and immunostimulating functions, which lead to numerous health benefits. Obtained re sults classify Se-polysaccharides as promising dietary supplements and Se-enriched mush rooms as delicious functional food or food additives, due to the slow release of selenium 
and lower toxicity compared to the inorganic compounds. Selenium fortified mushrooms 
and their extracts used as food additives are expected to prolong the shelf life of the food 
products since some of them are proved to exhibit higher superoxide dismutase and catalase 
activity retaining their quality, aroma, and color for a long time of storage.
PB  - SERBIAN NUTRITION SOCIETY
C3  - 14th international Congress of Nutrition (CONU2021)
T1  - Selenium enriched mushrooms as food additives and dietary supplements
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6645
ER  - 

@conference{
author = "Pantic, Milena and Miletić, Dunja and Matijašević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2021",
abstract = "Edible and medicinal mushrooms have been used worldwide due to their nutritional 
value and known pharmaceutical properties, which are related to the physiologically 
beneficial substances in the form of primary and secondary metabolites. Their chemical 
composition and biological activity depend on the medium on which they grow and applied 
supplementation. Numerous studies have shown that mushrooms can absorb selenium (Se) 
during growth on the fortified solid substrate or liquid medium, in a concentration that can 
meet the daily needs of humans. The capacity of some mushroom to absorb Se depends on 
multiple factors, including mushroom strain, composition of the medium, ecological pa rameters as well as on the form and concentration in which this mineral is added to the me dium. It was previously reported that strains Pleurotus sp, Agaricus bisporus, Ganoderma lu cidum, and Cordyceps militaris absorbed high concentrations of Se when sodium selenite or 
selenate were applied. However, higher toxicity of inorganic salts that suppressed mycelium 
growth of Hericium erinaceum, Lentinus edodes, Coriolus versicolor, and Pleurotus spp. com paring to modified zeolites with Se and organic selenitetriglycerides from Selol, Se-yeast, 
and selenourea was proven. Chemical characterization of polysaccharide and methanol ex tracts isolated from fruiting bodies or mycelia culture showed that most of the Se-enriched 
mushrooms had a higher content of total carbohydrates (especially β-glucans), proteins, fla vonoids, and phenols comparing to the non-enriched samples. As a consequence, selenized 
mushrooms showed improved antioxidant and antimicrobial properties. A significant per centage of Se was incorporated not only in the form of L-selenomethionine, but also other 
organic compounds, such as biologically active polysaccharides. It is believed that the syn ergism of Se and bioactive polysaccharides present in fungi could enhance their antitumor 
and immunostimulating functions, which lead to numerous health benefits. Obtained re sults classify Se-polysaccharides as promising dietary supplements and Se-enriched mush rooms as delicious functional food or food additives, due to the slow release of selenium 
and lower toxicity compared to the inorganic compounds. Selenium fortified mushrooms 
and their extracts used as food additives are expected to prolong the shelf life of the food 
products since some of them are proved to exhibit higher superoxide dismutase and catalase 
activity retaining their quality, aroma, and color for a long time of storage.",
publisher = "SERBIAN NUTRITION SOCIETY",
journal = "14th international Congress of Nutrition (CONU2021)",
title = "Selenium enriched mushrooms as food additives and dietary supplements",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6645"
}

Pantic, M., Miletić, D., Matijašević, D., Sknepnek, A.,& Nikšić, M.. (2021). Selenium enriched mushrooms as food additives and dietary supplements. in 14th international Congress of Nutrition (CONU2021)
SERBIAN NUTRITION SOCIETY., 71.
https://hdl.handle.net/21.15107/rcub_agrospace_6645

Pantic M, Miletić D, Matijašević D, Sknepnek A, Nikšić M. Selenium enriched mushrooms as food additives and dietary supplements. in 14th international Congress of Nutrition (CONU2021). 2021;:71.
https://hdl.handle.net/21.15107/rcub_agrospace_6645 .

Pantic, Milena, Miletić, Dunja, Matijašević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Selenium enriched mushrooms as food additives and dietary supplements" in 14th international Congress of Nutrition (CONU2021) (2021):71,
https://hdl.handle.net/21.15107/rcub_agrospace_6645 .

TY  - CONF
AU  - Miletić, Dunja
AU  - Sknepnek, Aleksandra
AU  - Milićević, Nataša
AU  - Pantić, Milena
AU  - Sakač, Marijana
AU  - Šarić, Bojana
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6835
AB  - Trametes versicolor, Lentinus edodes and Pleurotus ostreatus HK-35 mushrooms were used for the
production of mushroom-based cereal flours. Three different cereals were used for this purpose:
wheat (NS 40 S), rye (individual agricultural holding, Vojvodina province) and oat (Italico d.o.o).
Sterilized grains (pH 6.0 – 6.5, CaCO3) were seeded using mushroom's inoculum previously
prepared on the malt agar, and incubated at 25 ± 2 °C for 20-30 days in the dark, until the grains
were completely overgrown with mycelium. Control samples without seeding mushrooms’
mycelium were prepared as well. The obtained grains were dried and milled into flours, in order to
be used for the chemical characterization and antioxidative activity determination. All mushroombased grain flours characteristics were compared with corresponding controls. The highest total
carbohydrate content was determined for T. versicolor wheat (705.61±48.97 mg/g) and rye
(749.15±42.09 mg/g) grain flours, with an increase of 26.39±1.09 % and 39.78±13.42 % compared
to non-inoculated grains, respectively, while all inoculated flours had higher protein content
compared to non-inoculated ones. A significant carbohydrate content increase was observed for L.
edodes wheat grain flour (9.66±14.66 mg/g). Expand in total phenolic compounds content, between
7.72±0.39 and 217.74±54.65 %, was observed in eight out of nine tested samples, compared to the
control. The highest values of phenolic compounds were noted for T. versicolor oat (30.94±0.07
mg/g) and rye (27.45±2.34 mg/g) grain flour. A significant increase (p<0.05) of chelating ability
was observed for P. ostreatus and L. edodes wheat grain flours, when the very high chelating ability
for both samples (93.62±3.01 %) was observed. Significantly higher reducing power (p<0.05) was
detected in six out of nine tested samples, with the highest absorbance measured for L. edodes oat
(1.88±0.08) and rye (1.15±0.04) grain flours. Obtained results revealed that the growth of different
mushrooms on different cereals could be a promising method for enhancing the antioxidative
potential of flours.
PB  - University of Belgrade Studentski trg 1 11000 Belgrade
C3  - Unifood conference, Belgrade, Book of Abstracts
T1  - Development of mushroom-based cereal flours with improved  nutritional and antioxidative properties
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6835
ER  - 

@conference{
author = "Miletić, Dunja and Sknepnek, Aleksandra and Milićević, Nataša and Pantić, Milena and Sakač, Marijana and Šarić, Bojana and Nikšić, Miomir",
year = "2021",
abstract = "Trametes versicolor, Lentinus edodes and Pleurotus ostreatus HK-35 mushrooms were used for the
production of mushroom-based cereal flours. Three different cereals were used for this purpose:
wheat (NS 40 S), rye (individual agricultural holding, Vojvodina province) and oat (Italico d.o.o).
Sterilized grains (pH 6.0 – 6.5, CaCO3) were seeded using mushroom's inoculum previously
prepared on the malt agar, and incubated at 25 ± 2 °C for 20-30 days in the dark, until the grains
were completely overgrown with mycelium. Control samples without seeding mushrooms’
mycelium were prepared as well. The obtained grains were dried and milled into flours, in order to
be used for the chemical characterization and antioxidative activity determination. All mushroombased grain flours characteristics were compared with corresponding controls. The highest total
carbohydrate content was determined for T. versicolor wheat (705.61±48.97 mg/g) and rye
(749.15±42.09 mg/g) grain flours, with an increase of 26.39±1.09 % and 39.78±13.42 % compared
to non-inoculated grains, respectively, while all inoculated flours had higher protein content
compared to non-inoculated ones. A significant carbohydrate content increase was observed for L.
edodes wheat grain flour (9.66±14.66 mg/g). Expand in total phenolic compounds content, between
7.72±0.39 and 217.74±54.65 %, was observed in eight out of nine tested samples, compared to the
control. The highest values of phenolic compounds were noted for T. versicolor oat (30.94±0.07
mg/g) and rye (27.45±2.34 mg/g) grain flour. A significant increase (p<0.05) of chelating ability
was observed for P. ostreatus and L. edodes wheat grain flours, when the very high chelating ability
for both samples (93.62±3.01 %) was observed. Significantly higher reducing power (p<0.05) was
detected in six out of nine tested samples, with the highest absorbance measured for L. edodes oat
(1.88±0.08) and rye (1.15±0.04) grain flours. Obtained results revealed that the growth of different
mushrooms on different cereals could be a promising method for enhancing the antioxidative
potential of flours.",
publisher = "University of Belgrade Studentski trg 1 11000 Belgrade",
journal = "Unifood conference, Belgrade, Book of Abstracts",
title = "Development of mushroom-based cereal flours with improved  nutritional and antioxidative properties",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6835"
}

Miletić, D., Sknepnek, A., Milićević, N., Pantić, M., Sakač, M., Šarić, B.,& Nikšić, M.. (2021). Development of mushroom-based cereal flours with improved  nutritional and antioxidative properties. in Unifood conference, Belgrade, Book of Abstracts
University of Belgrade Studentski trg 1 11000 Belgrade., 168.
https://hdl.handle.net/21.15107/rcub_agrospace_6835

Miletić D, Sknepnek A, Milićević N, Pantić M, Sakač M, Šarić B, Nikšić M. Development of mushroom-based cereal flours with improved  nutritional and antioxidative properties. in Unifood conference, Belgrade, Book of Abstracts. 2021;:168.
https://hdl.handle.net/21.15107/rcub_agrospace_6835 .

Miletić, Dunja, Sknepnek, Aleksandra, Milićević, Nataša, Pantić, Milena, Sakač, Marijana, Šarić, Bojana, Nikšić, Miomir, "Development of mushroom-based cereal flours with improved  nutritional and antioxidative properties" in Unifood conference, Belgrade, Book of Abstracts (2021):168,
https://hdl.handle.net/21.15107/rcub_agrospace_6835 .

TY  - DATA
AU  - Sknepnek, Aleksandra
AU  - Tomić, Sergej
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Čolić, Miodrag
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5996
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary data for the article: Sknepnek, A.; Tomić, S.; Miletić, D.; Lević, S.; Čolić, M.; Nedović, V.; Nikšić, M. Fermentation Characteristics of Novel Coriolus Versicolor and Lentinus Edodes Kombucha Beverages and Immunomodulatory Potential of Their Polysaccharide Extracts. Food Chemistry 2021, 342, 128344. https://doi.org/10.1016/j.foodchem.2020.128344.
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5996
ER  - 

@misc{
author = "Sknepnek, Aleksandra and Tomić, Sergej and Miletić, Dunja and Lević, Steva and Čolić, Miodrag and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary data for the article: Sknepnek, A.; Tomić, S.; Miletić, D.; Lević, S.; Čolić, M.; Nedović, V.; Nikšić, M. Fermentation Characteristics of Novel Coriolus Versicolor and Lentinus Edodes Kombucha Beverages and Immunomodulatory Potential of Their Polysaccharide Extracts. Food Chemistry 2021, 342, 128344. https://doi.org/10.1016/j.foodchem.2020.128344.",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5996"
}

Sknepnek, A., Tomić, S., Miletić, D., Lević, S., Čolić, M., Nedović, V.,& Nikšić, M.. (2021). Supplementary data for the article: Sknepnek, A.; Tomić, S.; Miletić, D.; Lević, S.; Čolić, M.; Nedović, V.; Nikšić, M. Fermentation Characteristics of Novel Coriolus Versicolor and Lentinus Edodes Kombucha Beverages and Immunomodulatory Potential of Their Polysaccharide Extracts. Food Chemistry 2021, 342, 128344. https://doi.org/10.1016/j.foodchem.2020.128344.. in Food Chemistry
Elsevier..
https://hdl.handle.net/21.15107/rcub_agrospace_5996

Sknepnek A, Tomić S, Miletić D, Lević S, Čolić M, Nedović V, Nikšić M. Supplementary data for the article: Sknepnek, A.; Tomić, S.; Miletić, D.; Lević, S.; Čolić, M.; Nedović, V.; Nikšić, M. Fermentation Characteristics of Novel Coriolus Versicolor and Lentinus Edodes Kombucha Beverages and Immunomodulatory Potential of Their Polysaccharide Extracts. Food Chemistry 2021, 342, 128344. https://doi.org/10.1016/j.foodchem.2020.128344.. in Food Chemistry. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_5996 .

Sknepnek, Aleksandra, Tomić, Sergej, Miletić, Dunja, Lević, Steva, Čolić, Miodrag, Nedović, Viktor, Nikšić, Miomir, "Supplementary data for the article: Sknepnek, A.; Tomić, S.; Miletić, D.; Lević, S.; Čolić, M.; Nedović, V.; Nikšić, M. Fermentation Characteristics of Novel Coriolus Versicolor and Lentinus Edodes Kombucha Beverages and Immunomodulatory Potential of Their Polysaccharide Extracts. Food Chemistry 2021, 342, 128344. https://doi.org/10.1016/j.foodchem.2020.128344." in Food Chemistry (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_5996 .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Turło, Jadwiga
AU  - Podsadni, Piotr
AU  - Sknepnek, Aleksandra
AU  - Szczepanska, Agnieszka
AU  - Klimaszewska, Marzenna
AU  - Malinowska, Eliza
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5836
AB  - During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor
SP  - 101046
VL  - 42
DO  - 10.1016/j.fbio.2021.101046
ER  - 

@article{
author = "Miletić, Dunja and Turło, Jadwiga and Podsadni, Piotr and Sknepnek, Aleksandra and Szczepanska, Agnieszka and Klimaszewska, Marzenna and Malinowska, Eliza and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
abstract = "During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor",
pages = "101046",
volume = "42",
doi = "10.1016/j.fbio.2021.101046"
}

Miletić, D., Turło, J., Podsadni, P., Sknepnek, A., Szczepanska, A., Klimaszewska, M., Malinowska, E., Lević, S., Nedović, V.,& Nikšić, M.. (2021). Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience
Elsevier Ltd., 42, 101046.
https://doi.org/10.1016/j.fbio.2021.101046

Miletić D, Turło J, Podsadni P, Sknepnek A, Szczepanska A, Klimaszewska M, Malinowska E, Lević S, Nedović V, Nikšić M. Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience. 2021;42:101046.
doi:10.1016/j.fbio.2021.101046 .

Miletić, Dunja, Turło, Jadwiga, Podsadni, Piotr, Sknepnek, Aleksandra, Szczepanska, Agnieszka, Klimaszewska, Marzenna, Malinowska, Eliza, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor" in Food Bioscience, 42 (2021):101046,
https://doi.org/10.1016/j.fbio.2021.101046 . .

TY  - JOUR
AU  - Sknepnek, Aleksandra
AU  - Tomić, Sergej
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Čolić, Miodrag
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5772
AB  - Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.
PB  - Elsevier
T2  - Food Chemistry
T1  - Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts
SP  - 128344
VL  - 342
DO  - 10.1016/j.foodchem.2020.128344
ER  - 

@article{
author = "Sknepnek, Aleksandra and Tomić, Sergej and Miletić, Dunja and Lević, Steva and Čolić, Miodrag and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
abstract = "Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts",
pages = "128344",
volume = "342",
doi = "10.1016/j.foodchem.2020.128344"
}

Sknepnek, A., Tomić, S., Miletić, D., Lević, S., Čolić, M., Nedović, V.,& Nikšić, M.. (2021). Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts. in Food Chemistry
Elsevier., 342, 128344.
https://doi.org/10.1016/j.foodchem.2020.128344

Sknepnek A, Tomić S, Miletić D, Lević S, Čolić M, Nedović V, Nikšić M. Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts. in Food Chemistry. 2021;342:128344.
doi:10.1016/j.foodchem.2020.128344 .

Sknepnek, Aleksandra, Tomić, Sergej, Miletić, Dunja, Lević, Steva, Čolić, Miodrag, Nedović, Viktor, Nikšić, Miomir, "Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts" in Food Chemistry, 342 (2021):128344,
https://doi.org/10.1016/j.foodchem.2020.128344 . .

TY  - JOUR
AU  - Sknepnek, Aleksandra
AU  - Tomić, Sergej
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Čolić, Miodrag
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5771
AB  - Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.
PB  - Elsevier
T2  - Food Chemistry
T1  - Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts
SP  - 128344
VL  - 342
DO  - 10.1016/j.foodchem.2020.128344
ER  - 

@article{
author = "Sknepnek, Aleksandra and Tomić, Sergej and Miletić, Dunja and Lević, Steva and Čolić, Miodrag and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
abstract = "Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts",
pages = "128344",
volume = "342",
doi = "10.1016/j.foodchem.2020.128344"
}

Sknepnek, A., Tomić, S., Miletić, D., Lević, S., Čolić, M., Nedović, V.,& Nikšić, M.. (2021). Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts. in Food Chemistry
Elsevier., 342, 128344.
https://doi.org/10.1016/j.foodchem.2020.128344

Sknepnek A, Tomić S, Miletić D, Lević S, Čolić M, Nedović V, Nikšić M. Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts. in Food Chemistry. 2021;342:128344.
doi:10.1016/j.foodchem.2020.128344 .

Sknepnek, Aleksandra, Tomić, Sergej, Miletić, Dunja, Lević, Steva, Čolić, Miodrag, Nedović, Viktor, Nikšić, Miomir, "Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts" in Food Chemistry, 342 (2021):128344,
https://doi.org/10.1016/j.foodchem.2020.128344 . .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Turlo, Jadwiga
AU  - Podsadni, Piotr
AU  - Sknepnek, Aleksandra
AU  - Szczepanska, Agnieszka
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5287
AB  - Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing. Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower. However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order reducing power > DPPH scavenging ability > chelating ability. Due to the high carbohydrate and beta-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities. D-glucose was the main monosaccharide (87.18 +/- 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate. In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity
EP  - 895
IS  - 9
SP  - 885
VL  - 22
DO  - 10.1615/IntJMedMushrooms.2020035877
ER  - 

@article{
author = "Miletić, Dunja and Turlo, Jadwiga and Podsadni, Piotr and Sknepnek, Aleksandra and Szczepanska, Agnieszka and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2020",
abstract = "Crude Trametes versicolor exopolysaccharides (cEPS) were used for antioxidative activity testing. Obtained results revealed high ability of cEPS for DPPH free radical scavenging and high chelating ability at the highest tested concentration (20 mg/mL), while the reducing power was significantly lower. However, based on the EC50 values, antioxidative activities of the cEPS decreased in the following order reducing power > DPPH scavenging ability > chelating ability. Due to the high carbohydrate and beta-glucan content it is assumed that they are the main carriers of cEPS antioxidative activities. D-glucose was the main monosaccharide (87.18 +/- 0.27%) while the dominant amino acids were L-lysine (L-glutamic and L-aspartic acid), which are amino acids with taste similar to the monosodium glutamate. In addition, content of sweet tasting amino acids compared with the group of bitter tasting amino acid was 2.1 times higher, indicating favorable composition of cEPS protein fraction for food industry applying.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity",
pages = "895-885",
number = "9",
volume = "22",
doi = "10.1615/IntJMedMushrooms.2020035877"
}

Miletić, D., Turlo, J., Podsadni, P., Sknepnek, A., Szczepanska, A., Lević, S., Nedović, V.,& Nikšić, M.. (2020). Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 22(9), 885-895.
https://doi.org/10.1615/IntJMedMushrooms.2020035877

Miletić D, Turlo J, Podsadni P, Sknepnek A, Szczepanska A, Lević S, Nedović V, Nikšić M. Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity. in International Journal of Medicinal Mushrooms. 2020;22(9):885-895.
doi:10.1615/IntJMedMushrooms.2020035877 .

Miletić, Dunja, Turlo, Jadwiga, Podsadni, Piotr, Sknepnek, Aleksandra, Szczepanska, Agnieszka, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Turkey Tail Medicinal Mushroom, Trametes versicolor (Agaricomycetes), Crude Exopolysaccharides with Antioxidative Activity" in International Journal of Medicinal Mushrooms, 22, no. 9 (2020):885-895,
https://doi.org/10.1615/IntJMedMushrooms.2020035877 . .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Sknepnek, Aleksandra
AU  - Vasiljević, Ivana
AU  - Lazović, Milana
AU  - Nikšić, Miomir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5330
AB  - The ability of Coriolus versicolor medicinal mushroom to grow and accumulate selenium during submerged cultivation in a selenium-fortified medium is examined in this paper. For selenium supplementation, commercial selenium yeast was used. Control, nonenriched sample and reference cultures cultivated in the medium enriched with commercial yeast Saccharomyces cerevisiae were also prepared. The mushroom demonstrated a high ability to accumulate selenium from the added source (around 970 and 1,300 mu g/g of dry mycelium weight for samples enriched with selenium in a concentration of 10 and 20 mg Se/L, respectively). The addition of selenium significantly (p  lt = .05) increased the biomass yield, whereas the addition of nonenriched yeast had no significant (p  lt = .05) impact. Furthermore, regression analysis showed statistically significant (p  lt = .05) and positive correlations between the content of Se and Fe (r = .92), Se and Cu (r = .92), Se and Mn (r = .98), and Se and Sr (r = .96), suggesting that selenium incorporation was followed by incorporation of these elements, and led to mineral enrichment of the obtained mycelium. Methanol extracts prepared from mycelium biomass demonstrated a better inhibitory effect on Gram-positive bacterial strains with minimal inhibitory concentrations between  lt 0.3125 and 40 mg/ml. The obtained results showed that selenium yeast could be used for obtaining a potential novel food supplement: mushroom biomass with high selenium content and enhanced mineral composition.
PB  - Wiley, Hoboken
T2  - Journal of Basic Microbiology
T1  - Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom
EP  - 340
IS  - 4
SP  - 331
VL  - 60
DO  - 10.1002/jobm.201900520
ER  - 

@article{
author = "Miletić, Dunja and Pantić, Milena and Sknepnek, Aleksandra and Vasiljević, Ivana and Lazović, Milana and Nikšić, Miomir",
year = "2020",
abstract = "The ability of Coriolus versicolor medicinal mushroom to grow and accumulate selenium during submerged cultivation in a selenium-fortified medium is examined in this paper. For selenium supplementation, commercial selenium yeast was used. Control, nonenriched sample and reference cultures cultivated in the medium enriched with commercial yeast Saccharomyces cerevisiae were also prepared. The mushroom demonstrated a high ability to accumulate selenium from the added source (around 970 and 1,300 mu g/g of dry mycelium weight for samples enriched with selenium in a concentration of 10 and 20 mg Se/L, respectively). The addition of selenium significantly (p  lt = .05) increased the biomass yield, whereas the addition of nonenriched yeast had no significant (p  lt = .05) impact. Furthermore, regression analysis showed statistically significant (p  lt = .05) and positive correlations between the content of Se and Fe (r = .92), Se and Cu (r = .92), Se and Mn (r = .98), and Se and Sr (r = .96), suggesting that selenium incorporation was followed by incorporation of these elements, and led to mineral enrichment of the obtained mycelium. Methanol extracts prepared from mycelium biomass demonstrated a better inhibitory effect on Gram-positive bacterial strains with minimal inhibitory concentrations between  lt 0.3125 and 40 mg/ml. The obtained results showed that selenium yeast could be used for obtaining a potential novel food supplement: mushroom biomass with high selenium content and enhanced mineral composition.",
publisher = "Wiley, Hoboken",
journal = "Journal of Basic Microbiology",
title = "Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom",
pages = "340-331",
number = "4",
volume = "60",
doi = "10.1002/jobm.201900520"
}

Miletić, D., Pantić, M., Sknepnek, A., Vasiljević, I., Lazović, M.,& Nikšić, M.. (2020). Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom. in Journal of Basic Microbiology
Wiley, Hoboken., 60(4), 331-340.
https://doi.org/10.1002/jobm.201900520

Miletić D, Pantić M, Sknepnek A, Vasiljević I, Lazović M, Nikšić M. Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom. in Journal of Basic Microbiology. 2020;60(4):331-340.
doi:10.1002/jobm.201900520 .

Miletić, Dunja, Pantić, Milena, Sknepnek, Aleksandra, Vasiljević, Ivana, Lazović, Milana, Nikšić, Miomir, "Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom" in Journal of Basic Microbiology, 60, no. 4 (2020):331-340,
https://doi.org/10.1002/jobm.201900520 . .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Turlo, Jadwiga
AU  - Podsadni, Piotr
AU  - Pantić, Milena
AU  - Nedović, Viktor
AU  - Lević, Steva
AU  - Nikšić, Miomir
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4935
AB  - BACKGROUND:  The ability of Coriolus versicolor medicinal mushroom to accumulate and transform selenium from selenourea and sodium selenite into an organic form - l-selenomethionine - during growth in liquid medium is examined in this paper. Additionally, the impact of supplementation on biological activity of the selenated mushroom methanol extracts, as well as their chemical composition, is studied. RESULTS Selenium accumulation was more efficient with sodium selenite application, but biomass yield was significantly lower (1.89 g DW L-1) compared to samples enriched with selenourea (4.48 g DW L-1). Mushroom sample obtained after growing in liquid medium with selenourea had significantly higher l-selenomethionine content compared to the sample grown in medium with sodium selenite. Selenium-enriched methanol extracts of C. versicolor mushroom showed improved antimicrobial and antioxidant activities compared to non-enriched extract. CONCLUSION Our results suggest that C. versicolor mushroom cultivated in liquid culture enriched with selenourea can be used for the production of novel food supplements with improved selenium bioavailability. More than 30% of total accumulated selenium from selenourea is transformed into l-selenomethionine. Differences in biological activity of methanol extracts can be explained not only by different selenium content but also by the differences in chemical composition of extracts.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Selenium-enriched Coriolus versicolor mushroom biomass: potential novel food supplement with improved selenium bioavailability
EP  - 5130
IS  - 11
SP  - 5122
VL  - 99
DO  - 10.1002/jsfa.9756
ER  - 

@article{
author = "Miletić, Dunja and Turlo, Jadwiga and Podsadni, Piotr and Pantić, Milena and Nedović, Viktor and Lević, Steva and Nikšić, Miomir",
year = "2019",
abstract = "BACKGROUND:  The ability of Coriolus versicolor medicinal mushroom to accumulate and transform selenium from selenourea and sodium selenite into an organic form - l-selenomethionine - during growth in liquid medium is examined in this paper. Additionally, the impact of supplementation on biological activity of the selenated mushroom methanol extracts, as well as their chemical composition, is studied. RESULTS Selenium accumulation was more efficient with sodium selenite application, but biomass yield was significantly lower (1.89 g DW L-1) compared to samples enriched with selenourea (4.48 g DW L-1). Mushroom sample obtained after growing in liquid medium with selenourea had significantly higher l-selenomethionine content compared to the sample grown in medium with sodium selenite. Selenium-enriched methanol extracts of C. versicolor mushroom showed improved antimicrobial and antioxidant activities compared to non-enriched extract. CONCLUSION Our results suggest that C. versicolor mushroom cultivated in liquid culture enriched with selenourea can be used for the production of novel food supplements with improved selenium bioavailability. More than 30% of total accumulated selenium from selenourea is transformed into l-selenomethionine. Differences in biological activity of methanol extracts can be explained not only by different selenium content but also by the differences in chemical composition of extracts.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Selenium-enriched Coriolus versicolor mushroom biomass: potential novel food supplement with improved selenium bioavailability",
pages = "5130-5122",
number = "11",
volume = "99",
doi = "10.1002/jsfa.9756"
}

Miletić, D., Turlo, J., Podsadni, P., Pantić, M., Nedović, V., Lević, S.,& Nikšić, M.. (2019). Selenium-enriched Coriolus versicolor mushroom biomass: potential novel food supplement with improved selenium bioavailability. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 99(11), 5122-5130.
https://doi.org/10.1002/jsfa.9756

Miletić D, Turlo J, Podsadni P, Pantić M, Nedović V, Lević S, Nikšić M. Selenium-enriched Coriolus versicolor mushroom biomass: potential novel food supplement with improved selenium bioavailability. in Journal of the Science of Food and Agriculture. 2019;99(11):5122-5130.
doi:10.1002/jsfa.9756 .

Miletić, Dunja, Turlo, Jadwiga, Podsadni, Piotr, Pantić, Milena, Nedović, Viktor, Lević, Steva, Nikšić, Miomir, "Selenium-enriched Coriolus versicolor mushroom biomass: potential novel food supplement with improved selenium bioavailability" in Journal of the Science of Food and Agriculture, 99, no. 11 (2019):5122-5130,
https://doi.org/10.1002/jsfa.9756 . .

TY  - CONF
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Lević, Steva
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Pavlović, Vladimir
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6833
AB  - Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other
activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting
body and mycelia are recognized as potential functional food and dietary supplement. On the
market, there are already many products based on the fungi extracts. For certain types of
mushrooms, submerged production of bioactive compounds stands out as the best method from
the standpoint of industrial applications. Mushrooms are becoming an attractive functional food
and overview about type of food suitable for enrichment with this valuable compounds were
given.
The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate
bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth
(exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential.
During fermentation residual glucose was quantified by HPLC. Mycelia products were
characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of
isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative
bacteria strains using broth microdilution method. Results showed that after 48h glucose
concentration started to decrease and after day seven slowed down, when process was
terminated saving energy and time. Applied gentle stirring during cultivation provided constant
oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy
pellets with long, branched and anastomosed hyphae of high density were formed. In prepared
mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex
chemical composition of methanol extracts comparing to EPS was noted. That might be the
explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol
extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample
showed significant activity against both Gram-positive and Gram-negative strains. Based on
obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were
more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising
process for bioactive compounds production.
C3  - 2nd B-fost 2018 Congress 15-17 October Yerevan
T1  - Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6833
ER  - 

@conference{
author = "Miletić, Dunja and Pantić, Milena and Lević, Steva and Matijašević, Danka and Sknepnek, Aleksandra and Pavlović, Vladimir and Nedović, Viktor and Nikšić, Miomir",
year = "2018",
abstract = "Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other
activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting
body and mycelia are recognized as potential functional food and dietary supplement. On the
market, there are already many products based on the fungi extracts. For certain types of
mushrooms, submerged production of bioactive compounds stands out as the best method from
the standpoint of industrial applications. Mushrooms are becoming an attractive functional food
and overview about type of food suitable for enrichment with this valuable compounds were
given.
The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate
bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth
(exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential.
During fermentation residual glucose was quantified by HPLC. Mycelia products were
characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of
isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative
bacteria strains using broth microdilution method. Results showed that after 48h glucose
concentration started to decrease and after day seven slowed down, when process was
terminated saving energy and time. Applied gentle stirring during cultivation provided constant
oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy
pellets with long, branched and anastomosed hyphae of high density were formed. In prepared
mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex
chemical composition of methanol extracts comparing to EPS was noted. That might be the
explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol
extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample
showed significant activity against both Gram-positive and Gram-negative strains. Based on
obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were
more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising
process for bioactive compounds production.",
journal = "2nd B-fost 2018 Congress 15-17 October Yerevan",
title = "Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6833"
}

Miletić, D., Pantić, M., Lević, S., Matijašević, D., Sknepnek, A., Pavlović, V., Nedović, V.,& Nikšić, M.. (2018). Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production. in 2nd B-fost 2018 Congress 15-17 October Yerevan.
https://hdl.handle.net/21.15107/rcub_agrospace_6833

Miletić D, Pantić M, Lević S, Matijašević D, Sknepnek A, Pavlović V, Nedović V, Nikšić M. Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production. in 2nd B-fost 2018 Congress 15-17 October Yerevan. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_6833 .

Miletić, Dunja, Pantić, Milena, Lević, Steva, Matijašević, Danka, Sknepnek, Aleksandra, Pavlović, Vladimir, Nedović, Viktor, Nikšić, Miomir, "Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production" in 2nd B-fost 2018 Congress 15-17 October Yerevan (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_6833 .

TY  - CONF
AU  - Sknepnek, Aleksandra
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6845
AB  - Kombuha je fermentisani napitak koji potiče iz Mandčurije. U današnje vreme je postao veoma popularan i koristi se širom sveta. Vreli vodeni ekstrakti gljive Ganoderma lucidum je prvi put korišćen za pripremu kombuha produkta sa poboljšanim zdravstvenim efektom. Cilj ovog rada bio je određivanje hemijskog sastava novog napitka korišćenjem infracrvene spektroskopije sa Furijeovom transformacijom (FTIR) kao i ispitivanje njegove antioksidativne antibakterijske aktivnosti (ABA). Rezultati pokazuju da ovo piće može imati antibakterijsku aktivnost i zaštitnu ulogu kod oksidativnih oštećenja. Ovaj napitak se preporučuje kao funkcionalna hrana sa unapređenim zdravstvenim efektima i kao potencijalni atraktivni proizvod.
PB  - Zrenjanin
C3  - Saopštenje sa međunarodnog skupa štampano u izvodu
T1  - Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum
EP  - 61
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6845
ER  - 

@conference{
author = "Sknepnek, Aleksandra and Pantić, Milena and Matijašević, Danka and Miletić, Dunja and Lević, Steva and Nikšić, Miomir",
year = "2018",
abstract = "Kombuha je fermentisani napitak koji potiče iz Mandčurije. U današnje vreme je postao veoma popularan i koristi se širom sveta. Vreli vodeni ekstrakti gljive Ganoderma lucidum je prvi put korišćen za pripremu kombuha produkta sa poboljšanim zdravstvenim efektom. Cilj ovog rada bio je određivanje hemijskog sastava novog napitka korišćenjem infracrvene spektroskopije sa Furijeovom transformacijom (FTIR) kao i ispitivanje njegove antioksidativne antibakterijske aktivnosti (ABA). Rezultati pokazuju da ovo piće može imati antibakterijsku aktivnost i zaštitnu ulogu kod oksidativnih oštećenja. Ovaj napitak se preporučuje kao funkcionalna hrana sa unapređenim zdravstvenim efektima i kao potencijalni atraktivni proizvod.",
publisher = "Zrenjanin",
journal = "Saopštenje sa međunarodnog skupa štampano u izvodu",
title = "Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum",
pages = "61-60",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6845"
}

Sknepnek, A., Pantić, M., Matijašević, D., Miletić, D., Lević, S.,& Nikšić, M.. (2018). Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum. in Saopštenje sa međunarodnog skupa štampano u izvodu
Zrenjanin., 60-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6845

Sknepnek A, Pantić M, Matijašević D, Miletić D, Lević S, Nikšić M. Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum. in Saopštenje sa međunarodnog skupa štampano u izvodu. 2018;:60-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6845 .

Sknepnek, Aleksandra, Pantić, Milena, Matijašević, Danka, Miletić, Dunja, Lević, Steva, Nikšić, Miomir, "Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum" in Saopštenje sa međunarodnog skupa štampano u izvodu (2018):60-61,
https://hdl.handle.net/21.15107/rcub_agrospace_6845 .

TY  - CONF
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Miletić, Dunja
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6847
AB  - : Industrijski gajene gljive imaju veoma izraženu nutritivnu vrednost i predstavljaju bogat
izvor esencijalnih aminokiselina, vitamina, mineralnih materija, ugljenih hidrata, proteina kao i
važnih sekundarnih metabolita. Tokom višegodišnjih istraživanja dokazano je da gljiva može
uspešno usvojiti selen iz čvrstog supstrata ili tečne podloge u koncentraciji koja može da zadovolji
dnevne potrebe čoveka. Kapacitet usvojivosti selena zavisi od vrste gljive, hranljive podloge,
ekoloških parametara kao i od oblika i koncentraciјe u kom јe selen dodat u podlogu. Kako gljive
sadrže brojne bioaktivne komponente u vidu primarnih i sekundarnih metabolita, dodatno
selenom obogaćene pečurke moguće јe koristiti u svežem i sušenom stanju ili kao potencijalni
dijetetski suplement. Ispitana je biološka aktivnost selenom obogaćenih gljiva Coriolus versicolor
i Pleurotus ostreatus. Kao izvori selena upotrebljena su organska jedinjenja u obliku selenskog
kvasca i selenouree, kao i Se(IV)- i Se(VI)-modifikovani zeoliti.
PB  - Udruženje mikrobiologa Srbije, Nemanjina 6, Beograd
C3  - Predavanje po pozivu sa međunarodnog skupa štampano u celini
T1  - Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom
EP  - 152
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6847
ER  - 

@conference{
author = "Pantić, Milena and Matijašević, Danka and Miletić, Dunja and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2018",
abstract = ": Industrijski gajene gljive imaju veoma izraženu nutritivnu vrednost i predstavljaju bogat
izvor esencijalnih aminokiselina, vitamina, mineralnih materija, ugljenih hidrata, proteina kao i
važnih sekundarnih metabolita. Tokom višegodišnjih istraživanja dokazano je da gljiva može
uspešno usvojiti selen iz čvrstog supstrata ili tečne podloge u koncentraciji koja može da zadovolji
dnevne potrebe čoveka. Kapacitet usvojivosti selena zavisi od vrste gljive, hranljive podloge,
ekoloških parametara kao i od oblika i koncentraciјe u kom јe selen dodat u podlogu. Kako gljive
sadrže brojne bioaktivne komponente u vidu primarnih i sekundarnih metabolita, dodatno
selenom obogaćene pečurke moguće јe koristiti u svežem i sušenom stanju ili kao potencijalni
dijetetski suplement. Ispitana je biološka aktivnost selenom obogaćenih gljiva Coriolus versicolor
i Pleurotus ostreatus. Kao izvori selena upotrebljena su organska jedinjenja u obliku selenskog
kvasca i selenouree, kao i Se(IV)- i Se(VI)-modifikovani zeoliti.",
publisher = "Udruženje mikrobiologa Srbije, Nemanjina 6, Beograd",
journal = "Predavanje po pozivu sa međunarodnog skupa štampano u celini",
title = "Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom",
pages = "152-150",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6847"
}

Pantić, M., Matijašević, D., Miletić, D., Sknepnek, A.,& Nikšić, M.. (2018). Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom. in Predavanje po pozivu sa međunarodnog skupa štampano u celini
Udruženje mikrobiologa Srbije, Nemanjina 6, Beograd., 150-152.
https://hdl.handle.net/21.15107/rcub_agrospace_6847

Pantić M, Matijašević D, Miletić D, Sknepnek A, Nikšić M. Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom. in Predavanje po pozivu sa međunarodnog skupa štampano u celini. 2018;:150-152.
https://hdl.handle.net/21.15107/rcub_agrospace_6847 .

Pantić, Milena, Matijašević, Danka, Miletić, Dunja, Sknepnek, Aleksandra, Nikšić, Miomir, "Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom" in Predavanje po pozivu sa međunarodnog skupa štampano u celini (2018):150-152,
https://hdl.handle.net/21.15107/rcub_agrospace_6847 .

TY  - JOUR
AU  - Sknepnek, Aleksandra
AU  - Pantić, Milena
AU  - Matijasević, Danka
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4775
AB  - Kombucha is a nonalcoholic beverage traditionally made by fermenting black tea using a combination of yeast and acetic acid bacteria (AAB) cultures. Ganoderma lucidum hot water extract (HWE) was used-to our knowledge for the first time-to prepare a novel, health-promoting kombucha product. During the 11-day fermentation, pH, total acidity, and the numbers of yeasts and AAB were monitored. It was found that sweetened G. lucidum HWE was a good medium for yeast and AAB growth. The desired acidity for the beverage was reached on the second day (3 g/L) of the fermentation process; the maximum established acidity was 22.8 +/- 0.42 g/L. Fourier transform infrared analysis revealed that the vacuum-dried beverage is a mixture of various compounds such as polysaccharides, phenols, proteins, and lipids. Total phenolic content of the liquid sample was 4.91 +/- 0.2338 mg gallic acid equivalents/g, whereas the vacuum-dried sample had a smaller amount of phenolics (2.107 +/- 0.228 mg gallic acid equivalents/g). Established half-maximal effective concentrations for DPPH scavenging activity and reducing power were 22.8 +/- 0.17 and 10.61 +/- 0.34 mg/mL, respectively. The antibacterial testing revealed that activity does not originate solely from synthesized acetic acid. The liquid G. lucidum beverage was the most effective against the tested bacteria, with the lowest minimum inhibitory concentration (0.04 mg/ml,) against Staphylococcus epidermidis and Rhodococcus equi, and a minimum bactericidal concentration (0.16 mg/mL) against Bacillus spizizenii, B. cereus, and R. equi. The vacuum-dried sample was less effective, with the lowest minimum bactericidal concentration against the Gram-positive bacteria R. equi (1.875 mg/mL) and against the Gram-negative bacteria Proteus hauseri (30 mg/mL).
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects
EP  - 258
IS  - 3
SP  - 243
VL  - 20
DO  - 10.1615/IntJMedMushrooms.2018025833
ER  - 

@article{
author = "Sknepnek, Aleksandra and Pantić, Milena and Matijasević, Danka and Miletić, Dunja and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2018",
abstract = "Kombucha is a nonalcoholic beverage traditionally made by fermenting black tea using a combination of yeast and acetic acid bacteria (AAB) cultures. Ganoderma lucidum hot water extract (HWE) was used-to our knowledge for the first time-to prepare a novel, health-promoting kombucha product. During the 11-day fermentation, pH, total acidity, and the numbers of yeasts and AAB were monitored. It was found that sweetened G. lucidum HWE was a good medium for yeast and AAB growth. The desired acidity for the beverage was reached on the second day (3 g/L) of the fermentation process; the maximum established acidity was 22.8 +/- 0.42 g/L. Fourier transform infrared analysis revealed that the vacuum-dried beverage is a mixture of various compounds such as polysaccharides, phenols, proteins, and lipids. Total phenolic content of the liquid sample was 4.91 +/- 0.2338 mg gallic acid equivalents/g, whereas the vacuum-dried sample had a smaller amount of phenolics (2.107 +/- 0.228 mg gallic acid equivalents/g). Established half-maximal effective concentrations for DPPH scavenging activity and reducing power were 22.8 +/- 0.17 and 10.61 +/- 0.34 mg/mL, respectively. The antibacterial testing revealed that activity does not originate solely from synthesized acetic acid. The liquid G. lucidum beverage was the most effective against the tested bacteria, with the lowest minimum inhibitory concentration (0.04 mg/ml,) against Staphylococcus epidermidis and Rhodococcus equi, and a minimum bactericidal concentration (0.16 mg/mL) against Bacillus spizizenii, B. cereus, and R. equi. The vacuum-dried sample was less effective, with the lowest minimum bactericidal concentration against the Gram-positive bacteria R. equi (1.875 mg/mL) and against the Gram-negative bacteria Proteus hauseri (30 mg/mL).",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects",
pages = "258-243",
number = "3",
volume = "20",
doi = "10.1615/IntJMedMushrooms.2018025833"
}

Sknepnek, A., Pantić, M., Matijasević, D., Miletić, D., Lević, S., Nedović, V.,& Nikšić, M.. (2018). Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 20(3), 243-258.
https://doi.org/10.1615/IntJMedMushrooms.2018025833

Sknepnek A, Pantić M, Matijasević D, Miletić D, Lević S, Nedović V, Nikšić M. Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects. in International Journal of Medicinal Mushrooms. 2018;20(3):243-258.
doi:10.1615/IntJMedMushrooms.2018025833 .

Sknepnek, Aleksandra, Pantić, Milena, Matijasević, Danka, Miletić, Dunja, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Novel Kombucha Beverage from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, with Antibacterial and Antioxidant Effects" in International Journal of Medicinal Mushrooms, 20, no. 3 (2018):243-258,
https://doi.org/10.1615/IntJMedMushrooms.2018025833 . .

TY  - CONF
AU  - Pantić, Milena
AU  - Duvnjak, Dunja
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Tomasz, Słowiński
AU  - Turlo, Jadwiga
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6846
AB  - Fungal members of the class Basidiomycetes are recognized as potential sources of bioactive
compounds, which can be used in a possible replacement of the synthetic antioxidants and antibiotics
by the naturally originated ones. Genus Pleurotus includes over 50 species of edible and medicinal
mushrooms with great economic importance. Their fruiting bodies are rich in carbohydrates (28 -
50%), edible fibers (3-50%), protein (10 - 30%), essential amino acids (25-35%), vitamins (C, A, B, D),
minerals (K, P, Mg, Na, Fe, Zn) and lipids (3-5%).The aim of this research was to compare biological
characteristic of different extracts obtained from Oyster mushroom fruiting body, as well as to identify
the organic compounds responsible for detected activities and finally to summarize available data on
the factors that could affect on their antioxidant and antimicrobial activity. Samples of two types of
polysaccharide extracts, namely hot water (HW) and hot alkaline (HA) ones were prepared from
fruiting bodies of the industrially grown oyster mushroom Pleurotus ostreatus P80. Extracts were
examined for their antimicrobial and antioxidant biological activity. The antibacterial activity was the
widest against the Gram-positive bacteria Staphylococcus aureus for both types of polysaccharide
extracts. HA extract singled out as the most effective against Gram-negative bacteria Proteus hauseri
with microbicidal concentration of 2.5 mg mL–1
. HW polysaccharide extract was better antioxidant
which was shown through the lower EC50 values of DPPH scavenging ability and ferric-reducing
antioxidant power. EC50 values of the DPPH scavenging activity of the polysaccharides from HW and
HA extracts were found to be particularly low, 0.6 and 0.7 mg mL-1
, respectively. EC50 values of the
ferric-reducing antioxidant power were 4.3 and 4.5 mg mL-1 for HW and HA samples. Using
spectroscopic methods it was found that HW extracts contained more protein (399 mg g-1
) and
phenolic compounds (6.36 mg g-1
) compared to HA sample (20 mg g-1 protein and 4.96 mg g-1 phenolic
compounds). Structural FT-IR analysis also confirmed higher content of glucans in HW
polysaccharides.
PB  - University of Novi Sad Institute of Food Technology Bulevar cara Lazara 1; 21000 Novi Sad
C3  - Saopštenje sa međunarodnog skupa štampano u celini
T1  - Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain
EP  - 233
SP  - 229
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6846
ER  - 

@conference{
author = "Pantić, Milena and Duvnjak, Dunja and Matijašević, Danka and Sknepnek, Aleksandra and Tomasz, Słowiński and Turlo, Jadwiga and Nikšić, Miomir",
year = "2016",
abstract = "Fungal members of the class Basidiomycetes are recognized as potential sources of bioactive
compounds, which can be used in a possible replacement of the synthetic antioxidants and antibiotics
by the naturally originated ones. Genus Pleurotus includes over 50 species of edible and medicinal
mushrooms with great economic importance. Their fruiting bodies are rich in carbohydrates (28 -
50%), edible fibers (3-50%), protein (10 - 30%), essential amino acids (25-35%), vitamins (C, A, B, D),
minerals (K, P, Mg, Na, Fe, Zn) and lipids (3-5%).The aim of this research was to compare biological
characteristic of different extracts obtained from Oyster mushroom fruiting body, as well as to identify
the organic compounds responsible for detected activities and finally to summarize available data on
the factors that could affect on their antioxidant and antimicrobial activity. Samples of two types of
polysaccharide extracts, namely hot water (HW) and hot alkaline (HA) ones were prepared from
fruiting bodies of the industrially grown oyster mushroom Pleurotus ostreatus P80. Extracts were
examined for their antimicrobial and antioxidant biological activity. The antibacterial activity was the
widest against the Gram-positive bacteria Staphylococcus aureus for both types of polysaccharide
extracts. HA extract singled out as the most effective against Gram-negative bacteria Proteus hauseri
with microbicidal concentration of 2.5 mg mL–1
. HW polysaccharide extract was better antioxidant
which was shown through the lower EC50 values of DPPH scavenging ability and ferric-reducing
antioxidant power. EC50 values of the DPPH scavenging activity of the polysaccharides from HW and
HA extracts were found to be particularly low, 0.6 and 0.7 mg mL-1
, respectively. EC50 values of the
ferric-reducing antioxidant power were 4.3 and 4.5 mg mL-1 for HW and HA samples. Using
spectroscopic methods it was found that HW extracts contained more protein (399 mg g-1
) and
phenolic compounds (6.36 mg g-1
) compared to HA sample (20 mg g-1 protein and 4.96 mg g-1 phenolic
compounds). Structural FT-IR analysis also confirmed higher content of glucans in HW
polysaccharides.",
publisher = "University of Novi Sad Institute of Food Technology Bulevar cara Lazara 1; 21000 Novi Sad",
journal = "Saopštenje sa međunarodnog skupa štampano u celini",
title = "Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain",
pages = "233-229",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6846"
}

Pantić, M., Duvnjak, D., Matijašević, D., Sknepnek, A., Tomasz, S., Turlo, J.,& Nikšić, M.. (2016). Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain. in Saopštenje sa međunarodnog skupa štampano u celini
University of Novi Sad Institute of Food Technology Bulevar cara Lazara 1; 21000 Novi Sad., 229-233.
https://hdl.handle.net/21.15107/rcub_agrospace_6846

Pantić M, Duvnjak D, Matijašević D, Sknepnek A, Tomasz S, Turlo J, Nikšić M. Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain. in Saopštenje sa međunarodnog skupa štampano u celini. 2016;:229-233.
https://hdl.handle.net/21.15107/rcub_agrospace_6846 .

Pantić, Milena, Duvnjak, Dunja, Matijašević, Danka, Sknepnek, Aleksandra, Tomasz, Słowiński, Turlo, Jadwiga, Nikšić, Miomir, "Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain" in Saopštenje sa međunarodnog skupa štampano u celini (2016):229-233,
https://hdl.handle.net/21.15107/rcub_agrospace_6846 .

TY  - JOUR
AU  - Matijasević, Danka
AU  - Pantić, Milena
AU  - Rašković, Božidar
AU  - Pavlović, Vladimir
AU  - Miletić, Dunja
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4017
AB  - The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis
VL  - 7
DO  - 10.3389/fmicb.2016.01226
ER  - 

@article{
author = "Matijasević, Danka and Pantić, Milena and Rašković, Božidar and Pavlović, Vladimir and Miletić, Dunja and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2016",
abstract = "The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis",
volume = "7",
doi = "10.3389/fmicb.2016.01226"
}

Matijasević, D., Pantić, M., Rašković, B., Pavlović, V., Miletić, D., Sknepnek, A.,& Nikšić, M.. (2016). The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.01226

Matijasević D, Pantić M, Rašković B, Pavlović V, Miletić D, Sknepnek A, Nikšić M. The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.01226 .

Matijasević, Danka, Pantić, Milena, Rašković, Božidar, Pavlović, Vladimir, Miletić, Dunja, Sknepnek, Aleksandra, Nikšić, Miomir, "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.01226 . .

TY  - JOUR
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Nedović, Viktor
AU  - Lević, Steva
AU  - Matijasević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4157
AB  - Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC)  lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Innovative Food Science & Emerging Technologies
T1  - Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds
EP  - 8
SP  - 1
VL  - 34
DO  - 10.1016/j.ifset.2015.12.028
ER  - 

@article{
author = "Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Nedović, Viktor and Lević, Steva and Matijasević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2016",
abstract = "Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC)  lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Innovative Food Science & Emerging Technologies",
title = "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds",
pages = "8-1",
volume = "34",
doi = "10.1016/j.ifset.2015.12.028"
}

Miletić, D., Pantić, M., Pavlović, V., Nedović, V., Lević, S., Matijasević, D., Sknepnek, A.,& Nikšić, M.. (2016). Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies
Elsevier Sci Ltd, Oxford., 34, 1-8.
https://doi.org/10.1016/j.ifset.2015.12.028

Miletić D, Pantić M, Pavlović V, Nedović V, Lević S, Matijasević D, Sknepnek A, Nikšić M. Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies. 2016;34:1-8.
doi:10.1016/j.ifset.2015.12.028 .

Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Nedović, Viktor, Lević, Steva, Matijasević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds" in Innovative Food Science & Emerging Technologies, 34 (2016):1-8,
https://doi.org/10.1016/j.ifset.2015.12.028 . .

TY  - CONF
AU  - Duvnjak, Dunja
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Jovanović, Ljubinko
AU  - Vasiljević, Ivana
AU  - Lazović, Milana
AU  - Nikšić, Miomir
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6834
AB  - Coriolus versicolor je medicinska gljiva čija je lekovitost potvrđena
brojnim istraživanjima. Biološki aktivni polisaharidi i polisaharopeptidi
izolovani iz micelije submerzno gajene Coriolus versicolor gljive se
koriste u prevenciji mnogih bolesti. Ova gljiva se može gajiti i na čvrstom
supstratu, na otpadu iz poljoprivrede i na taj način se može uspešno
upotrebiti pri bioremedijaciji. Cilj ovog istraživanja je bio da se utvrdi da
li se antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus
versicolor gajenjem na čvrstom supstratu obogaćenom selenom može
povećati.
Kao supstrat za gajenje upotrebljena je mešavina slame, hrastove pljevine
i pšeničnih mekinja. Za obogaćivanje supstrata korišćeni su organski i
neogranski izvori selena: selesnki kvasac (Sel-Plex®
, Alltech Inc.,
Lexington, USA) i natrijum selenit, Na2SeO3 u koncentraciji od 25 mg/kg
suvog supstrata. Kontrolni uzorak je pripremljen bez dodavanja selena.
Nakon metanolne ekstrakcije, određen je sadržaj selena u digestovanim
uzorcima upotrebom ICP-OES uređaja. Kod uzorka obogaćenih selenom
iz neorganske soli sadržaj selena je bio 123,4 µg/g, a kod uzoraka
obogaćenih selenom iz selenskog kvasca 62,4 µg/g suve mase uzorka.
Mikrodilucionom metodom ispitana je antimikrobna aktivnost dobijenih
metanolnih ekstrakata na 15 sojeva bakterija i utvrđeno je da dodatak
neorganske soli dovodi do povećanja antimikrobne aktivnosti u odnosu na
kontrolni uzorak samo kod dve Gram-pozitivne bakterije: Bacillus
spizizeni ATCC 6633 gde je MIC<0,3125 mg/ml i Enerococcus faecalis
ATCC 29212, MIC=40 mg/ml. Sa druge stane, ispitivanjem
antimikrobnog delovanja ekstrakata dobijenih iz gljive obogaćene selenom iz kvasca primećeno je izraženije mikrobistatično i mikrobicidno
delovanje u poređenju i sa kontrolnim uzorkom i sa uzorkom kome je
dodat Na2SeO3 kod skoro svih testiranih Gram-pozitivnih i Gramnegativnih bakterija.
Iako dobijeni rezultati ukazuju na to da je u ekstraktu gljive koja je rasla
na supstratu sa selenskim kvascem detektovano duplo manje ukupnog
selena nego kod uzorka obogaćenog selenitom, antimikrobna aktivnost
takvih uzoraka je bila znatno izraženija. Pretpostavlja se da je selen iz
organskog izvora promenio hemijski sastav gljive.
PB  - UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd
C3  - Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije
T1  - Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.
EP  - 201
SP  - 200
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6834
ER  - 

@conference{
author = "Duvnjak, Dunja and Pantić, Milena and Matijašević, Danka and Jovanović, Ljubinko and Vasiljević, Ivana and Lazović, Milana and Nikšić, Miomir",
year = "2015",
abstract = "Coriolus versicolor je medicinska gljiva čija je lekovitost potvrđena
brojnim istraživanjima. Biološki aktivni polisaharidi i polisaharopeptidi
izolovani iz micelije submerzno gajene Coriolus versicolor gljive se
koriste u prevenciji mnogih bolesti. Ova gljiva se može gajiti i na čvrstom
supstratu, na otpadu iz poljoprivrede i na taj način se može uspešno
upotrebiti pri bioremedijaciji. Cilj ovog istraživanja je bio da se utvrdi da
li se antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus
versicolor gajenjem na čvrstom supstratu obogaćenom selenom može
povećati.
Kao supstrat za gajenje upotrebljena je mešavina slame, hrastove pljevine
i pšeničnih mekinja. Za obogaćivanje supstrata korišćeni su organski i
neogranski izvori selena: selesnki kvasac (Sel-Plex®
, Alltech Inc.,
Lexington, USA) i natrijum selenit, Na2SeO3 u koncentraciji od 25 mg/kg
suvog supstrata. Kontrolni uzorak je pripremljen bez dodavanja selena.
Nakon metanolne ekstrakcije, određen je sadržaj selena u digestovanim
uzorcima upotrebom ICP-OES uređaja. Kod uzorka obogaćenih selenom
iz neorganske soli sadržaj selena je bio 123,4 µg/g, a kod uzoraka
obogaćenih selenom iz selenskog kvasca 62,4 µg/g suve mase uzorka.
Mikrodilucionom metodom ispitana je antimikrobna aktivnost dobijenih
metanolnih ekstrakata na 15 sojeva bakterija i utvrđeno je da dodatak
neorganske soli dovodi do povećanja antimikrobne aktivnosti u odnosu na
kontrolni uzorak samo kod dve Gram-pozitivne bakterije: Bacillus
spizizeni ATCC 6633 gde je MIC<0,3125 mg/ml i Enerococcus faecalis
ATCC 29212, MIC=40 mg/ml. Sa druge stane, ispitivanjem
antimikrobnog delovanja ekstrakata dobijenih iz gljive obogaćene selenom iz kvasca primećeno je izraženije mikrobistatično i mikrobicidno
delovanje u poređenju i sa kontrolnim uzorkom i sa uzorkom kome je
dodat Na2SeO3 kod skoro svih testiranih Gram-pozitivnih i Gramnegativnih bakterija.
Iako dobijeni rezultati ukazuju na to da je u ekstraktu gljive koja je rasla
na supstratu sa selenskim kvascem detektovano duplo manje ukupnog
selena nego kod uzorka obogaćenog selenitom, antimikrobna aktivnost
takvih uzoraka je bila znatno izraženija. Pretpostavlja se da je selen iz
organskog izvora promenio hemijski sastav gljive.",
publisher = "UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd",
journal = "Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije",
title = "Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.",
pages = "201-200",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6834"
}

Duvnjak, D., Pantić, M., Matijašević, D., Jovanović, L., Vasiljević, I., Lazović, M.,& Nikšić, M.. (2015). Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.. in Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije
UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd., 200-201.
https://hdl.handle.net/21.15107/rcub_agrospace_6834

Duvnjak D, Pantić M, Matijašević D, Jovanović L, Vasiljević I, Lazović M, Nikšić M. Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.. in Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije. 2015;:200-201.
https://hdl.handle.net/21.15107/rcub_agrospace_6834 .

Duvnjak, Dunja, Pantić, Milena, Matijašević, Danka, Jovanović, Ljubinko, Vasiljević, Ivana, Lazović, Milana, Nikšić, Miomir, "Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena." in Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije (2015):200-201,
https://hdl.handle.net/21.15107/rcub_agrospace_6834 .

TY  - JOUR
AU  - Savić, Milena
AU  - Andjelković, I.
AU  - Duvnjak, Dunja
AU  - Matijasević, Danka
AU  - Avramović, Aleksandra
AU  - Nikšić, Miomir
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3061
AB  - The activity of organic selenium against pathogenic molds and its use as a potential selenium source in the production of enriched mushrooms were examined. The effect of commercial selenized yeast on mycelia growth was examined using a method with mycelia disks and a well diffusion method. For mushroom enrichment, different concentrations of selenium were added to a growth substrate. The results presented in this paper suggest that the most suitable concentration of selenized yeast that inhibits the growth of the mycopathogenic molds is 70-100 mg/kg of selenium. With the addition of this concentration to the substrate, mushroom fruit bodies will uptake a high level of selenium, about 100 μg/g for Pleurotus spp., and 200 μg/g for Agaricus bisporus in dry weight of the mushroom. Thereby a double effect in the cultivation of mushrooms is achieved.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.
EP  - 1463
IS  - 4
SP  - 1455
VL  - 64
DO  - 10.2298/ABS1204455S
ER  - 

@article{
author = "Savić, Milena and Andjelković, I. and Duvnjak, Dunja and Matijasević, Danka and Avramović, Aleksandra and Nikšić, Miomir",
year = "2012",
abstract = "The activity of organic selenium against pathogenic molds and its use as a potential selenium source in the production of enriched mushrooms were examined. The effect of commercial selenized yeast on mycelia growth was examined using a method with mycelia disks and a well diffusion method. For mushroom enrichment, different concentrations of selenium were added to a growth substrate. The results presented in this paper suggest that the most suitable concentration of selenized yeast that inhibits the growth of the mycopathogenic molds is 70-100 mg/kg of selenium. With the addition of this concentration to the substrate, mushroom fruit bodies will uptake a high level of selenium, about 100 μg/g for Pleurotus spp., and 200 μg/g for Agaricus bisporus in dry weight of the mushroom. Thereby a double effect in the cultivation of mushrooms is achieved.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.",
pages = "1463-1455",
number = "4",
volume = "64",
doi = "10.2298/ABS1204455S"
}

Savić, M., Andjelković, I., Duvnjak, D., Matijasević, D., Avramović, A.,& Nikšić, M.. (2012). The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1455-1463.
https://doi.org/10.2298/ABS1204455S

Savić M, Andjelković I, Duvnjak D, Matijasević D, Avramović A, Nikšić M. The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp.. in Archives of Biological Sciences. 2012;64(4):1455-1463.
doi:10.2298/ABS1204455S .

Savić, Milena, Andjelković, I., Duvnjak, Dunja, Matijasević, Danka, Avramović, Aleksandra, Nikšić, Miomir, "The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp." in Archives of Biological Sciences, 64, no. 4 (2012):1455-1463,
https://doi.org/10.2298/ABS1204455S . .