TY  - CONF
AU  - Pantić, Milena
AU  - Kozarski, Maja
AU  - Lazić, Vesna
AU  - Nikšić, Miomir
AU  - Daković, Aleksandra
AU  - Krajišnik, Danina
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6696
AB  - Chitosan is a natural biopolymer that could be used in clay modification to adsorb mycotoxins, secondary 
metabolites of filamentous fungi commonly found in cereal grains and animal feed. Only a small number 
of mushrooms have chitosan, which is mostly produced from chitin through the deacetylation process. 
In this work, chitosan was extracted from the fruiting bodies of commercially grown Agaricus bisporus
with 9 M NaOH for 1.5 h in an autoclave followed by the treatment with 5% acetic acid (150 °C, 6 h), 
precipitated under alkaline conditions and neutralized. The antioxidant properties of prepared chitosan 
were determined under in vitro conditions by using spectrophotometric tests, while antibacterial activity 
was tested using broth microdilution assay (C= 0.078-5 mg/ml conc). The mycotoxigenic strain of 
Aspergillus flavus was grown on a solid medium enriched with 20 mg/ml of chitosan. The percentage of 
inhibition of the mycelium growth compared with the control was calculated. Additionally, the composite 
of bentonite with chitosan isolated from the mushroom was prepared and modified with surfactant 
hexadecyltrimethylammonium bromide. Adsorption of mycotoxin zearalenone using composite at pH 
3 by HPLC was determined.
The scavenging ability of free DPPH•
 radicals of the laboratory-prepared chitosan at a concentration of 
5 mg/mL was 99.35%, while the ability to neutralize ABTS+• radical cations was in the range of 31-94%, 
depending on the concentration. The ferrous ions chelating ability of this sample was 98.50% at 5 mg/
mL. Low bactericidal concentrations were detected, 2.5 mg/ml for Escherichia coli (ATCC 25922) and 
0.625 mg/ml for Enterococcus faecalis (ATCC 29219). A. flavus was inhibited for approximately 36% 
after three days of growth on the agar with chitosan, compared with control. Clay-chitosan- surfactant 
composite adsorbed 91.75% of zearalenone, compared to the clay-chitosan composite (7.25%), which 
means that surfactant molecules have a main role in toxin adsorption. The antioxidative and antimicrobial 
activity of crude chitosan from mushrooms makes it a potential material for food packaging, while a 
clay-chitosan-surfactant composite could be used for decontamination of animal feed contaminated 
with mycotoxins
C3  - rd B-Fost 2023 Congress (Black Sea Association of Food science and Technology) and Congress of Food Technologists of Serbia
T1  - Antimicrobial and antioxidant properties of crude chitosan extracted  from cultivated Agaricus bisporus
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6696
ER  - 

@conference{
author = "Pantić, Milena and Kozarski, Maja and Lazić, Vesna and Nikšić, Miomir and Daković, Aleksandra and Krajišnik, Danina",
year = "2023",
abstract = "Chitosan is a natural biopolymer that could be used in clay modification to adsorb mycotoxins, secondary 
metabolites of filamentous fungi commonly found in cereal grains and animal feed. Only a small number 
of mushrooms have chitosan, which is mostly produced from chitin through the deacetylation process. 
In this work, chitosan was extracted from the fruiting bodies of commercially grown Agaricus bisporus
with 9 M NaOH for 1.5 h in an autoclave followed by the treatment with 5% acetic acid (150 °C, 6 h), 
precipitated under alkaline conditions and neutralized. The antioxidant properties of prepared chitosan 
were determined under in vitro conditions by using spectrophotometric tests, while antibacterial activity 
was tested using broth microdilution assay (C= 0.078-5 mg/ml conc). The mycotoxigenic strain of 
Aspergillus flavus was grown on a solid medium enriched with 20 mg/ml of chitosan. The percentage of 
inhibition of the mycelium growth compared with the control was calculated. Additionally, the composite 
of bentonite with chitosan isolated from the mushroom was prepared and modified with surfactant 
hexadecyltrimethylammonium bromide. Adsorption of mycotoxin zearalenone using composite at pH 
3 by HPLC was determined.
The scavenging ability of free DPPH•
 radicals of the laboratory-prepared chitosan at a concentration of 
5 mg/mL was 99.35%, while the ability to neutralize ABTS+• radical cations was in the range of 31-94%, 
depending on the concentration. The ferrous ions chelating ability of this sample was 98.50% at 5 mg/
mL. Low bactericidal concentrations were detected, 2.5 mg/ml for Escherichia coli (ATCC 25922) and 
0.625 mg/ml for Enterococcus faecalis (ATCC 29219). A. flavus was inhibited for approximately 36% 
after three days of growth on the agar with chitosan, compared with control. Clay-chitosan- surfactant 
composite adsorbed 91.75% of zearalenone, compared to the clay-chitosan composite (7.25%), which 
means that surfactant molecules have a main role in toxin adsorption. The antioxidative and antimicrobial 
activity of crude chitosan from mushrooms makes it a potential material for food packaging, while a 
clay-chitosan-surfactant composite could be used for decontamination of animal feed contaminated 
with mycotoxins",
journal = "rd B-Fost 2023 Congress (Black Sea Association of Food science and Technology) and Congress of Food Technologists of Serbia",
title = "Antimicrobial and antioxidant properties of crude chitosan extracted  from cultivated Agaricus bisporus",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6696"
}

Pantić, M., Kozarski, M., Lazić, V., Nikšić, M., Daković, A.,& Krajišnik, D.. (2023). Antimicrobial and antioxidant properties of crude chitosan extracted  from cultivated Agaricus bisporus. in rd B-Fost 2023 Congress (Black Sea Association of Food science and Technology) and Congress of Food Technologists of Serbia, 26.
https://hdl.handle.net/21.15107/rcub_agrospace_6696

Pantić M, Kozarski M, Lazić V, Nikšić M, Daković A, Krajišnik D. Antimicrobial and antioxidant properties of crude chitosan extracted  from cultivated Agaricus bisporus. in rd B-Fost 2023 Congress (Black Sea Association of Food science and Technology) and Congress of Food Technologists of Serbia. 2023;:26.
https://hdl.handle.net/21.15107/rcub_agrospace_6696 .

Pantić, Milena, Kozarski, Maja, Lazić, Vesna, Nikšić, Miomir, Daković, Aleksandra, Krajišnik, Danina, "Antimicrobial and antioxidant properties of crude chitosan extracted  from cultivated Agaricus bisporus" in rd B-Fost 2023 Congress (Black Sea Association of Food science and Technology) and Congress of Food Technologists of Serbia (2023):26,
https://hdl.handle.net/21.15107/rcub_agrospace_6696 .

TY  - JOUR
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Lazić, Vesna
AU  - Kozarski, Maja
AU  - Radić, Danka
AU  - Šovljanski, Olja
AU  - Pezo, Lato
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6323
AB  - The problem of microbial biofilms has come to the fore alongside food, pharmaceutical, and healthcare industrialization. The development of new antibiofilm products has become urgent, but it includes bioprospecting and is time and money-consuming. Contemporary efforts are directed at the pursuit of effective compounds of natural origin, also known as “green” agents. Mushrooms appear to be a possible new source of antibiofilm compounds, as has been demonstrated recently. The existing modeling methods are directed toward predicting bacterial biofilm formation, not in the presence of antibiofilm materials. Moreover, the modeling is almost exclusively targeted at biofilms in healthcare, while modeling related to the food industry remains under-researched. The present study applied an Artificial Neural Network (ANN) model to analyze the anti-adhesion and anti-biofilm-forming effects of 40 extracts from 20 mushroom species against two very important food-borne bacterial species for food and food-related industries—Listeria monocytogenes and Salmonella enteritidis. The models developed in this study exhibited high prediction quality, as indicated by high r2 values during the training cycle. The best fit between the modeled and measured values was observed for the inhibition of adhesion. This study provides a valuable contribution to the field, supporting industrial settings during the initial stage of biofilm formation, when these communities are the most vulnerable, and promoting innovative and improved safety management. © 2023 by the authors.
T2  - Antibiotics
T2  - Antibiotics
T1  - Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens
IS  - 3
VL  - 12
DO  - 10.3390/antibiotics12030627
ER  - 

@article{
author = "Vunduk, Jovana and Klaus, Anita and Lazić, Vesna and Kozarski, Maja and Radić, Danka and Šovljanski, Olja and Pezo, Lato",
year = "2023",
abstract = "The problem of microbial biofilms has come to the fore alongside food, pharmaceutical, and healthcare industrialization. The development of new antibiofilm products has become urgent, but it includes bioprospecting and is time and money-consuming. Contemporary efforts are directed at the pursuit of effective compounds of natural origin, also known as “green” agents. Mushrooms appear to be a possible new source of antibiofilm compounds, as has been demonstrated recently. The existing modeling methods are directed toward predicting bacterial biofilm formation, not in the presence of antibiofilm materials. Moreover, the modeling is almost exclusively targeted at biofilms in healthcare, while modeling related to the food industry remains under-researched. The present study applied an Artificial Neural Network (ANN) model to analyze the anti-adhesion and anti-biofilm-forming effects of 40 extracts from 20 mushroom species against two very important food-borne bacterial species for food and food-related industries—Listeria monocytogenes and Salmonella enteritidis. The models developed in this study exhibited high prediction quality, as indicated by high r2 values during the training cycle. The best fit between the modeled and measured values was observed for the inhibition of adhesion. This study provides a valuable contribution to the field, supporting industrial settings during the initial stage of biofilm formation, when these communities are the most vulnerable, and promoting innovative and improved safety management. © 2023 by the authors.",
journal = "Antibiotics, Antibiotics",
title = "Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens",
number = "3",
volume = "12",
doi = "10.3390/antibiotics12030627"
}

Vunduk, J., Klaus, A., Lazić, V., Kozarski, M., Radić, D., Šovljanski, O.,& Pezo, L.. (2023). Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens. in Antibiotics, 12(3).
https://doi.org/10.3390/antibiotics12030627

Vunduk J, Klaus A, Lazić V, Kozarski M, Radić D, Šovljanski O, Pezo L. Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens. in Antibiotics. 2023;12(3).
doi:10.3390/antibiotics12030627 .

Vunduk, Jovana, Klaus, Anita, Lazić, Vesna, Kozarski, Maja, Radić, Danka, Šovljanski, Olja, Pezo, Lato, "Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens" in Antibiotics, 12, no. 3 (2023),
https://doi.org/10.3390/antibiotics12030627 . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - van Griensven, Leo
AU  - Jakovljevic, Dragica
AU  - Todorovic, Nina
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Vunduk, Jovana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6269
AB  - Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet. These valuable organisms are more than food, they fit in perfectly as a novel market group known as nutra-mycoceuticals. Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry. Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients. This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers, β-glucans. The literature data on their bioavailability, metabolic transformations, preclinical and human clinical research, and safety are discussed. Immunomodulatory mechanisms of polyphenol ingredients are also considered. These molecules present great potential in the design of the new immunity balancer formulations according to their widespread structural diversity. Finally, we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity. © 2022
T2  - Food Science and Human Wellness
T2  - Food Science and Human Wellness
T1  - Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application
EP  - 396
IS  - 2
SP  - 378
VL  - 12
DO  - 10.1016/j.fshw.2022.07.040
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and van Griensven, Leo and Jakovljevic, Dragica and Todorovic, Nina and Wan-Mohtar, Wan Abd Al Qadr Imad and Vunduk, Jovana",
year = "2023",
abstract = "Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet. These valuable organisms are more than food, they fit in perfectly as a novel market group known as nutra-mycoceuticals. Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry. Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients. This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers, β-glucans. The literature data on their bioavailability, metabolic transformations, preclinical and human clinical research, and safety are discussed. Immunomodulatory mechanisms of polyphenol ingredients are also considered. These molecules present great potential in the design of the new immunity balancer formulations according to their widespread structural diversity. Finally, we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity. © 2022",
journal = "Food Science and Human Wellness, Food Science and Human Wellness",
title = "Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application",
pages = "396-378",
number = "2",
volume = "12",
doi = "10.1016/j.fshw.2022.07.040"
}

Kozarski, M., Klaus, A., van Griensven, L., Jakovljevic, D., Todorovic, N., Wan-Mohtar, W. A. A. Q. I.,& Vunduk, J.. (2023). Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application. in Food Science and Human Wellness, 12(2), 378-396.
https://doi.org/10.1016/j.fshw.2022.07.040

Kozarski M, Klaus A, van Griensven L, Jakovljevic D, Todorovic N, Wan-Mohtar WAAQI, Vunduk J. Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application. in Food Science and Human Wellness. 2023;12(2):378-396.
doi:10.1016/j.fshw.2022.07.040 .

Kozarski, Maja, Klaus, Anita, van Griensven, Leo, Jakovljevic, Dragica, Todorovic, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, Vunduk, Jovana, "Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application" in Food Science and Human Wellness, 12, no. 2 (2023):378-396,
https://doi.org/10.1016/j.fshw.2022.07.040 . .

TY  - CONF
AU  - Lazić, Vesna
AU  - Ilić, Aleksandra
AU  - Dunčević, Marina
AU  - Kozarski, Maja
AU  - Nikšić, Miomir
AU  - Pantić, Milena
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6545
AB  - Chitosan is a linear polysaccharide formed from β-D-glucosamine randomly distributed chains and
N-acetyl-D-glucosamine, which is obtained in the process of deacetylation of chitin. Only a small number
of mushrooms have chitosan as one of the components in their cell wall. It is assumed to exhibit
antimicrobial, antifungal and antiviral activity. The task of this research was to test the antioxidant and
antibacterial activity of commercial products chitosan (Aldrich, Germany) and chitosan hydrochloride
(Glentham Life Sciences, UK). The degree of deacetylation of both products was about 85%. Commercially,
Agaricus bisporus is mostly used for chitosan production.
The antibacterial potential was tested by the microdilution method (2.5-0.019 mg/mL) using one
Gram-positive bacteria Enterococcus faecalis ATCC 29219 and one Gram-negative Escherichia coli
ATCC 25922 and the reduction of the number of microorganisms was monitored using the highest tested
concentrations of 2.5 mg/mL samples. Chitosan and chitosan hydrochloride showed a significant
effect on the tested microorganisms. The minimal inhibitory (MIC) concentration of chitosan hydrochloride
against E. coli was 1.25 mg/mL, while a concentration of 2.5 mg/mL was the minimal bactericidal
concentration (MBC) for the same bacteria, while the MIC against E. faecalis was a concentration of
2.5 mg/mL. There was also a complete reduction in the number of E. coli. Commercial chitosan had a
stronger effect on E. faecalis (MIC 1.25 and MBC 2.5 mg/mL), than on E. coli where a significant growth
inhibition was also observed (MIC 2.5 mg/mL, MBC was not detected for the tested concentrations).
The function of antioxidants is to remove reactive oxygen species and to prevent the occurrence and
spread of oxidative stress. By examining the antioxidant activity, the absence of DPPH radical scavenging
ability was determined for the tested samples with a concentration of 5mg/ml, while the ability to
chelate Fe2+ was low and amounted to 27.22 % for chitosan and 41.56 % for chitosan hydrochloride.
In further research, it is necessary to find the best way to extract chitosan from mushrooms in order to
obtain samples with pronounced biological activity.
C3  - 11th International Medicinal Mushroom Conference (IMMC11)
T1  - Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6545
ER  - 

@conference{
author = "Lazić, Vesna and Ilić, Aleksandra and Dunčević, Marina and Kozarski, Maja and Nikšić, Miomir and Pantić, Milena",
year = "2022",
abstract = "Chitosan is a linear polysaccharide formed from β-D-glucosamine randomly distributed chains and
N-acetyl-D-glucosamine, which is obtained in the process of deacetylation of chitin. Only a small number
of mushrooms have chitosan as one of the components in their cell wall. It is assumed to exhibit
antimicrobial, antifungal and antiviral activity. The task of this research was to test the antioxidant and
antibacterial activity of commercial products chitosan (Aldrich, Germany) and chitosan hydrochloride
(Glentham Life Sciences, UK). The degree of deacetylation of both products was about 85%. Commercially,
Agaricus bisporus is mostly used for chitosan production.
The antibacterial potential was tested by the microdilution method (2.5-0.019 mg/mL) using one
Gram-positive bacteria Enterococcus faecalis ATCC 29219 and one Gram-negative Escherichia coli
ATCC 25922 and the reduction of the number of microorganisms was monitored using the highest tested
concentrations of 2.5 mg/mL samples. Chitosan and chitosan hydrochloride showed a significant
effect on the tested microorganisms. The minimal inhibitory (MIC) concentration of chitosan hydrochloride
against E. coli was 1.25 mg/mL, while a concentration of 2.5 mg/mL was the minimal bactericidal
concentration (MBC) for the same bacteria, while the MIC against E. faecalis was a concentration of
2.5 mg/mL. There was also a complete reduction in the number of E. coli. Commercial chitosan had a
stronger effect on E. faecalis (MIC 1.25 and MBC 2.5 mg/mL), than on E. coli where a significant growth
inhibition was also observed (MIC 2.5 mg/mL, MBC was not detected for the tested concentrations).
The function of antioxidants is to remove reactive oxygen species and to prevent the occurrence and
spread of oxidative stress. By examining the antioxidant activity, the absence of DPPH radical scavenging
ability was determined for the tested samples with a concentration of 5mg/ml, while the ability to
chelate Fe2+ was low and amounted to 27.22 % for chitosan and 41.56 % for chitosan hydrochloride.
In further research, it is necessary to find the best way to extract chitosan from mushrooms in order to
obtain samples with pronounced biological activity.",
journal = "11th International Medicinal Mushroom Conference (IMMC11)",
title = "Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6545"
}

Lazić, V., Ilić, A., Dunčević, M., Kozarski, M., Nikšić, M.,& Pantić, M.. (2022). Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms. in 11th International Medicinal Mushroom Conference (IMMC11).
https://hdl.handle.net/21.15107/rcub_agrospace_6545

Lazić V, Ilić A, Dunčević M, Kozarski M, Nikšić M, Pantić M. Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms. in 11th International Medicinal Mushroom Conference (IMMC11). 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6545 .

Lazić, Vesna, Ilić, Aleksandra, Dunčević, Marina, Kozarski, Maja, Nikšić, Miomir, Pantić, Milena, "Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms" in 11th International Medicinal Mushroom Conference (IMMC11) (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6545 .

TY  - GEN
AU  - Lazić, Vesna
AU  - Ilić, Aleksandra
AU  - Dunčević, Marina
AU  - Kozarski, Maja
AU  - Nikšić, Miomir
AU  - Pantić, Milena
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6620
AB  - Chitosan is a linear polysaccharide formed from β-D-glucosamine randomly distributed chains and
N-acetyl-D-glucosamine, which is obtained in the process of deacetylation of chitin. Only a small number
of mushrooms have chitosan as one of the components in their cell wall. It is assumed to exhibit
antimicrobial, antifungal and antiviral activity. The task of this research was to test the antioxidant and
antibacterial activity of commercial products chitosan (Aldrich, Germany) and chitosan hydrochloride
(Glentham Life Sciences, UK). The degree of deacetylation of both products was about 85%. Commercially,
Agaricus bisporus is mostly used for chitosan production.
The antibacterial potential was tested by the microdilution method (2.5-0.019 mg/mL) using one
Gram-positive bacteria Enterococcus faecalis ATCC 29219 and one Gram-negative Escherichia coli
ATCC 25922 and the reduction of the number of microorganisms was monitored using the highest tested
concentrations of 2.5 mg/mL samples. Chitosan and chitosan hydrochloride showed a significant
effect on the tested microorganisms. The minimal inhibitory (MIC) concentration of chitosan hydrochloride
against E. coli was 1.25 mg/mL, while a concentration of 2.5 mg/mL was the minimal bactericidal
concentration (MBC) for the same bacteria, while the MIC against E. faecalis was a concentration of
2.5 mg/mL. There was also a complete reduction in the number of E. coli. Commercial chitosan had a
stronger effect on E. faecalis (MIC 1.25 and MBC 2.5 mg/mL), than on E. coli where a significant growth
inhibition was also observed (MIC 2.5 mg/mL, MBC was not detected for the tested concentrations).
The function of antioxidants is to remove reactive oxygen species and to prevent the occurrence and
spread of oxidative stress. By examining the antioxidant activity, the absence of DPPH radical scavenging
ability was determined for the tested samples with a concentration of 5mg/ml, while the ability to
chelate Fe2+ was low and amounted to 27.22 % for chitosan and 41.56 % for chitosan hydrochloride.
In further research, it is necessary to find the best way to extract chitosan from mushrooms in order to
obtain samples with pronounced biological activity.
T2  - 11th International Medicinal Mushroom Conference (IMMC11)
T1  - Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6620
ER  - 

@misc{
author = "Lazić, Vesna and Ilić, Aleksandra and Dunčević, Marina and Kozarski, Maja and Nikšić, Miomir and Pantić, Milena",
year = "2022",
abstract = "Chitosan is a linear polysaccharide formed from β-D-glucosamine randomly distributed chains and
N-acetyl-D-glucosamine, which is obtained in the process of deacetylation of chitin. Only a small number
of mushrooms have chitosan as one of the components in their cell wall. It is assumed to exhibit
antimicrobial, antifungal and antiviral activity. The task of this research was to test the antioxidant and
antibacterial activity of commercial products chitosan (Aldrich, Germany) and chitosan hydrochloride
(Glentham Life Sciences, UK). The degree of deacetylation of both products was about 85%. Commercially,
Agaricus bisporus is mostly used for chitosan production.
The antibacterial potential was tested by the microdilution method (2.5-0.019 mg/mL) using one
Gram-positive bacteria Enterococcus faecalis ATCC 29219 and one Gram-negative Escherichia coli
ATCC 25922 and the reduction of the number of microorganisms was monitored using the highest tested
concentrations of 2.5 mg/mL samples. Chitosan and chitosan hydrochloride showed a significant
effect on the tested microorganisms. The minimal inhibitory (MIC) concentration of chitosan hydrochloride
against E. coli was 1.25 mg/mL, while a concentration of 2.5 mg/mL was the minimal bactericidal
concentration (MBC) for the same bacteria, while the MIC against E. faecalis was a concentration of
2.5 mg/mL. There was also a complete reduction in the number of E. coli. Commercial chitosan had a
stronger effect on E. faecalis (MIC 1.25 and MBC 2.5 mg/mL), than on E. coli where a significant growth
inhibition was also observed (MIC 2.5 mg/mL, MBC was not detected for the tested concentrations).
The function of antioxidants is to remove reactive oxygen species and to prevent the occurrence and
spread of oxidative stress. By examining the antioxidant activity, the absence of DPPH radical scavenging
ability was determined for the tested samples with a concentration of 5mg/ml, while the ability to
chelate Fe2+ was low and amounted to 27.22 % for chitosan and 41.56 % for chitosan hydrochloride.
In further research, it is necessary to find the best way to extract chitosan from mushrooms in order to
obtain samples with pronounced biological activity.",
journal = "11th International Medicinal Mushroom Conference (IMMC11)",
title = "Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms",
pages = "105",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6620"
}

Lazić, V., Ilić, A., Dunčević, M., Kozarski, M., Nikšić, M.,& Pantić, M.. (2022). Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms. in 11th International Medicinal Mushroom Conference (IMMC11), 105.
https://hdl.handle.net/21.15107/rcub_agrospace_6620

Lazić V, Ilić A, Dunčević M, Kozarski M, Nikšić M, Pantić M. Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms. in 11th International Medicinal Mushroom Conference (IMMC11). 2022;:105.
https://hdl.handle.net/21.15107/rcub_agrospace_6620 .

Lazić, Vesna, Ilić, Aleksandra, Dunčević, Marina, Kozarski, Maja, Nikšić, Miomir, Pantić, Milena, "Antibacterial and antioxidative activity of chitosan and chitosan hydrochloride isolated from mushrooms" in 11th International Medicinal Mushroom Conference (IMMC11) (2022):105,
https://hdl.handle.net/21.15107/rcub_agrospace_6620 .

TY  - CONF
AU  - Kozarski, Maja
AU  - Pantic, Milena
AU  - Lazic, Vesna
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Griensven, Leo
AU  - Niksic, Miomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6543
AB  - Mushroom polyphenols have been proposed as a new therapy that can improve the immune system.
Different immune cells express multiple types of polyphenol receptors that recognize and allow cellular
uptake of polyphenols, which subsequently activate signaling pathways to initiate immune responses.
Polyphenols can be useful for immunocompromised patients with malignancies, viral or bacterial
infections and chronic disorders of organ systems, as well as for those whose immunity is naturally
weakened. Cosmetic formulations rich in mushroom polyphenols have the potential to regulate inflammatory
skin disorders, as well as eczema or atopic dermatitis and photocarcinogenesis.
As primary antioxidants, polyphenols have an important role in preserving immune cells in a reduced
environment and in protecting them against oxidative damages and immunosuppression as well as
maintaining their suitable function. As signaling pathway modulators, mushroom polyphenols can
affect immune cell regulation, cytokine synthesis, and gene expression, in both innate and adaptive
immune responses. There are different pathways in the integrated immunomodulatory polyphenol response,
such as: 1) the nuclear NF-κB signaling pathway leading to suppression of various inflammatory
cytokines expression, and enzymes such as COX-2; 2) MAPK signaling pathways that play a key role in
many fundamental cellular processes such as cell growth, proliferation, death and differentiation, and
also regulate gene transcription and the activity of transcription factors involved in inflammation; 3)
arachidonic acid signaling pathway leading to a decrease in the release of inflammatory mediators;
and 4) the Nrf2 / ARE signaling pathway involved in the activation of genes encoding cytoprotective and
antioxidant enzymes.
Given that polyphenols represent great potential in the design of immune-boosting formulations in line
with their widespread structural diversity, it should be noted that some functionality issues require further
clarification. For instance different observations and conclusions were reported by the scientists in
absence of enzymes involved in their biosynthesis steps.
Concerning the dietary application of mushroom polyphenols, it is necessary to mention that after
oral consumption they are recognized by the human body as xenobiotics and often a small amount is
absorbed in the intestine. Likewise, there are significant differences between the activities of the metabolic
form of phenols and their form in the mushroom nutraceutical matrix. For an example, dihydoferulic
acid, a metabolite of the ferulic acid, expresses anti-inflammatory activity, opposite to metabolites derived
after sulfation and glucuronidation. The scientific focus is on improving and increasing polyphenols bioavailability
by designing colloidal systems and using nanosystems. Moreover many studies have found
that polyphenols can interact with macromolecules like dietary fibers, i.e. chitin and β-glucans. It has
been shown that these interactions can affect the bioaccessibility of polyphenols in a food matrix as
well as in nutraceutical formulations. Therefore, chitin and β-glucans have an impact on polyphenols’
immunomodulating activities if they were applied together in commercial formulations. Another disadvantage
in the commercialization of polyphenol-based formulations is quantitative and qualitative variations
in their content among different mushroom species. As secondary metabolites with a protective
role e.g. phenols, their content in mushrooms depends on the locality and environmental conditions of
growth. Using extracts or preparations that are based on mycelia grown under controlled conditions
may be the solution for the reduction of the natural variability in polyphenol composition.
Integrated information on all aspects of functionality will confirm the use of polyphenol-rich mushroom
formulations as effective enhancers of immunity.
C3  - 11th International Medicinal Mushroom Conference (IMMC11)
T1  - Mushroom polyphenols as immune system balancers: What's the mechanism behind it and possible interactions with dietary fibers?
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6543
ER  - 

@conference{
author = "Kozarski, Maja and Pantic, Milena and Lazic, Vesna and Klaus, Anita and Vunduk, Jovana and Griensven, Leo and Niksic, Miomir",
year = "2022",
abstract = "Mushroom polyphenols have been proposed as a new therapy that can improve the immune system.
Different immune cells express multiple types of polyphenol receptors that recognize and allow cellular
uptake of polyphenols, which subsequently activate signaling pathways to initiate immune responses.
Polyphenols can be useful for immunocompromised patients with malignancies, viral or bacterial
infections and chronic disorders of organ systems, as well as for those whose immunity is naturally
weakened. Cosmetic formulations rich in mushroom polyphenols have the potential to regulate inflammatory
skin disorders, as well as eczema or atopic dermatitis and photocarcinogenesis.
As primary antioxidants, polyphenols have an important role in preserving immune cells in a reduced
environment and in protecting them against oxidative damages and immunosuppression as well as
maintaining their suitable function. As signaling pathway modulators, mushroom polyphenols can
affect immune cell regulation, cytokine synthesis, and gene expression, in both innate and adaptive
immune responses. There are different pathways in the integrated immunomodulatory polyphenol response,
such as: 1) the nuclear NF-κB signaling pathway leading to suppression of various inflammatory
cytokines expression, and enzymes such as COX-2; 2) MAPK signaling pathways that play a key role in
many fundamental cellular processes such as cell growth, proliferation, death and differentiation, and
also regulate gene transcription and the activity of transcription factors involved in inflammation; 3)
arachidonic acid signaling pathway leading to a decrease in the release of inflammatory mediators;
and 4) the Nrf2 / ARE signaling pathway involved in the activation of genes encoding cytoprotective and
antioxidant enzymes.
Given that polyphenols represent great potential in the design of immune-boosting formulations in line
with their widespread structural diversity, it should be noted that some functionality issues require further
clarification. For instance different observations and conclusions were reported by the scientists in
absence of enzymes involved in their biosynthesis steps.
Concerning the dietary application of mushroom polyphenols, it is necessary to mention that after
oral consumption they are recognized by the human body as xenobiotics and often a small amount is
absorbed in the intestine. Likewise, there are significant differences between the activities of the metabolic
form of phenols and their form in the mushroom nutraceutical matrix. For an example, dihydoferulic
acid, a metabolite of the ferulic acid, expresses anti-inflammatory activity, opposite to metabolites derived
after sulfation and glucuronidation. The scientific focus is on improving and increasing polyphenols bioavailability
by designing colloidal systems and using nanosystems. Moreover many studies have found
that polyphenols can interact with macromolecules like dietary fibers, i.e. chitin and β-glucans. It has
been shown that these interactions can affect the bioaccessibility of polyphenols in a food matrix as
well as in nutraceutical formulations. Therefore, chitin and β-glucans have an impact on polyphenols’
immunomodulating activities if they were applied together in commercial formulations. Another disadvantage
in the commercialization of polyphenol-based formulations is quantitative and qualitative variations
in their content among different mushroom species. As secondary metabolites with a protective
role e.g. phenols, their content in mushrooms depends on the locality and environmental conditions of
growth. Using extracts or preparations that are based on mycelia grown under controlled conditions
may be the solution for the reduction of the natural variability in polyphenol composition.
Integrated information on all aspects of functionality will confirm the use of polyphenol-rich mushroom
formulations as effective enhancers of immunity.",
journal = "11th International Medicinal Mushroom Conference (IMMC11)",
title = "Mushroom polyphenols as immune system balancers: What's the mechanism behind it and possible interactions with dietary fibers?",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6543"
}

Kozarski, M., Pantic, M., Lazic, V., Klaus, A., Vunduk, J., Griensven, L.,& Niksic, M.. (2022). Mushroom polyphenols as immune system balancers: What's the mechanism behind it and possible interactions with dietary fibers?. in 11th International Medicinal Mushroom Conference (IMMC11).
https://hdl.handle.net/21.15107/rcub_agrospace_6543

Kozarski M, Pantic M, Lazic V, Klaus A, Vunduk J, Griensven L, Niksic M. Mushroom polyphenols as immune system balancers: What's the mechanism behind it and possible interactions with dietary fibers?. in 11th International Medicinal Mushroom Conference (IMMC11). 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6543 .

Kozarski, Maja, Pantic, Milena, Lazic, Vesna, Klaus, Anita, Vunduk, Jovana, Griensven, Leo, Niksic, Miomir, "Mushroom polyphenols as immune system balancers: What's the mechanism behind it and possible interactions with dietary fibers?" in 11th International Medicinal Mushroom Conference (IMMC11) (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6543 .

TY  - CONF
AU  - Lazić, Vesna
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Pantić, Milena
AU  - Kozarski, Maja
AU  - Nikšić, Miomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6546
AB  - Inonotus obliquus, or Chaga, is a medicinal mushroom with a long history of folk medicinal use and growing popularity worldwide. It cannot be cultivated so novel, greener extraction methods are needed to satisfy the market and obtain high-quality supplements.  In this study, we evaluated the biological activity (antimicrobial and antioxidant) of Mongolian (IM) and Serbian (IS) Chaga extracts obtained by subcritical water extraction. They were also screened for general chemical composition. Both IM and IS, have high phenolic content. HPLC analysis revealed that extracts are especially rich in chlorogenic acid, followed by catechins, p-coumaric and cinnamic acids. ABTS assay confirmed the remarkable scavenging ability of extracts, reaching 98% when a concentration of      2 mg/mL  was tested. The antimicrobial potential was examined by microdilution method using Gram-positive and (Enterococcus faecalis and Staphylococcus aureus), Gram-negative bacteria (Escherichia coli and Actinetobacteria baumannii). According to the obtained minimal inhibitory concentration (MIC) for E. faecalis S. aureus (MIC -1.25 mg / mL), E. coli, and A. baumannii (MIC -2.5 mg / mL) both extracts were shown to have the same effect on the tested bacteria. Hereby, we demonstrated the high biological potential of extracts obtained by subcritical water extraction.
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - The effect of green extraction method, subcritical, water extraction, on selected biological activities of Chaga mushroom
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6546
ER  - 

@conference{
author = "Lazić, Vesna and Vunduk, Jovana and Klaus, Anita and Pantić, Milena and Kozarski, Maja and Nikšić, Miomir",
year = "2022",
abstract = "Inonotus obliquus, or Chaga, is a medicinal mushroom with a long history of folk medicinal use and growing popularity worldwide. It cannot be cultivated so novel, greener extraction methods are needed to satisfy the market and obtain high-quality supplements.  In this study, we evaluated the biological activity (antimicrobial and antioxidant) of Mongolian (IM) and Serbian (IS) Chaga extracts obtained by subcritical water extraction. They were also screened for general chemical composition. Both IM and IS, have high phenolic content. HPLC analysis revealed that extracts are especially rich in chlorogenic acid, followed by catechins, p-coumaric and cinnamic acids. ABTS assay confirmed the remarkable scavenging ability of extracts, reaching 98% when a concentration of      2 mg/mL  was tested. The antimicrobial potential was examined by microdilution method using Gram-positive and (Enterococcus faecalis and Staphylococcus aureus), Gram-negative bacteria (Escherichia coli and Actinetobacteria baumannii). According to the obtained minimal inhibitory concentration (MIC) for E. faecalis S. aureus (MIC -1.25 mg / mL), E. coli, and A. baumannii (MIC -2.5 mg / mL) both extracts were shown to have the same effect on the tested bacteria. Hereby, we demonstrated the high biological potential of extracts obtained by subcritical water extraction.",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "The effect of green extraction method, subcritical, water extraction, on selected biological activities of Chaga mushroom",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6546"
}

Lazić, V., Vunduk, J., Klaus, A., Pantić, M., Kozarski, M.,& Nikšić, M.. (2022). The effect of green extraction method, subcritical, water extraction, on selected biological activities of Chaga mushroom. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology.
https://hdl.handle.net/21.15107/rcub_agrospace_6546

Lazić V, Vunduk J, Klaus A, Pantić M, Kozarski M, Nikšić M. The effect of green extraction method, subcritical, water extraction, on selected biological activities of Chaga mushroom. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6546 .

Lazić, Vesna, Vunduk, Jovana, Klaus, Anita, Pantić, Milena, Kozarski, Maja, Nikšić, Miomir, "The effect of green extraction method, subcritical, water extraction, on selected biological activities of Chaga mushroom" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6546 .

TY  - CONF
AU  - Lazić, Vesna
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Pantić, Milena
AU  - Kozarski, Maja
AU  - Nikšić, Miomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6626
AB  - Inonotus obliquus, or Chaga, is a medicinal mushroom with a long history of folk medicinal use and growing popularity worldwide. It cannot be cultivated so novel, greener extraction methods are needed to satisfy the market and obtain high-quality supplements.  In this study, we evaluated the biological activity (antimicrobial and antioxidant) of Mongolian (IM) and Serbian (IS) Chaga extracts obtained by subcritical water extraction. They were also screened for general chemical composition. Both IM and IS, have high phenolic content. HPLC analysis revealed that extracts are especially rich in chlorogenic acid, followed by catechins, p-coumaric and cinnamic acids. ABTS assay confirmed the remarkable scavenging ability of extracts, reaching 98% when a concentration of      2 mg/mL  was tested. The antimicrobial potential was examined by microdilution method using Gram-positive and (Enterococcus faecalis and Staphylococcus aureus), Gram-negative bacteria (Escherichia coli and Actinetobacteria baumannii). According to the obtained minimal inhibitory concentration (MIC) for E. faecalis S. aureus (MIC -1.25 mg / mL), E. coli, and A. baumannii (MIC -2.5 mg / mL) both extracts were shown to have the same effect on the tested bacteria. Hereby, we demonstrated the high biological potential of extracts obtained by subcritical water extraction.
PB  - Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - The effect of green extraction method, subcritical, water extraction, on selected biological activities in Chaga mushroom
SP  - 420
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6626
ER  - 

@conference{
author = "Lazić, Vesna and Vunduk, Jovana and Klaus, Anita and Pantić, Milena and Kozarski, Maja and Nikšić, Miomir",
year = "2022",
abstract = "Inonotus obliquus, or Chaga, is a medicinal mushroom with a long history of folk medicinal use and growing popularity worldwide. It cannot be cultivated so novel, greener extraction methods are needed to satisfy the market and obtain high-quality supplements.  In this study, we evaluated the biological activity (antimicrobial and antioxidant) of Mongolian (IM) and Serbian (IS) Chaga extracts obtained by subcritical water extraction. They were also screened for general chemical composition. Both IM and IS, have high phenolic content. HPLC analysis revealed that extracts are especially rich in chlorogenic acid, followed by catechins, p-coumaric and cinnamic acids. ABTS assay confirmed the remarkable scavenging ability of extracts, reaching 98% when a concentration of      2 mg/mL  was tested. The antimicrobial potential was examined by microdilution method using Gram-positive and (Enterococcus faecalis and Staphylococcus aureus), Gram-negative bacteria (Escherichia coli and Actinetobacteria baumannii). According to the obtained minimal inhibitory concentration (MIC) for E. faecalis S. aureus (MIC -1.25 mg / mL), E. coli, and A. baumannii (MIC -2.5 mg / mL) both extracts were shown to have the same effect on the tested bacteria. Hereby, we demonstrated the high biological potential of extracts obtained by subcritical water extraction.",
publisher = "Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "The effect of green extraction method, subcritical, water extraction, on selected biological activities in Chaga mushroom",
pages = "420",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6626"
}

Lazić, V., Vunduk, J., Klaus, A., Pantić, M., Kozarski, M.,& Nikšić, M.. (2022). The effect of green extraction method, subcritical, water extraction, on selected biological activities in Chaga mushroom. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Serbian Society of Microbiology., 420.
https://hdl.handle.net/21.15107/rcub_agrospace_6626

Lazić V, Vunduk J, Klaus A, Pantić M, Kozarski M, Nikšić M. The effect of green extraction method, subcritical, water extraction, on selected biological activities in Chaga mushroom. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:420.
https://hdl.handle.net/21.15107/rcub_agrospace_6626 .

Lazić, Vesna, Vunduk, Jovana, Klaus, Anita, Pantić, Milena, Kozarski, Maja, Nikšić, Miomir, "The effect of green extraction method, subcritical, water extraction, on selected biological activities in Chaga mushroom" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):420,
https://hdl.handle.net/21.15107/rcub_agrospace_6626 .

TY  - CONF
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Lazić, Vesna
AU  - Spirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Spasić, Snežana
AU  - Jakovljević, Dragica
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6550
AB  - Excessive production of reactive oxygen species (ROS) often results in irreversible cell
damage and leads to a number of disorders such as hypertension, heart disease, diabetes,
inflammation, premature aging and cancer. Considering the importance of diet in prevention
of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant
activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural
source of functional food ingredients. Nowadays functional food products based on the
lignicolous mushroom species are increasingly available on the market. Their powders and
extracts are consumed as dietary supplements in the form of capsules or tablets and as
additives in the formulation of healthier food products.
Antioxidant capacity of F. pinicola methanol extract was evaluated in vitro by the inhibition
of lipid peroxidation (LPx) in the linoleic acid model system. Results were normalized and
expressed as EC50 values (μg/mL). The Folin-Ciocalteu reaction adapted for a 96-well
microplate reader was used to determine the total phenol content (TPC) and result was
expressed as mg of gallic acid equivalent (GAE) per g of dry weight (DW) of extract.
Phenolic profile of the extract was analyzed by the liquid chromatography coupled with
tandem mass spectrometry (LC-MS/MS). Based on the analysis, TPC of the methanol extract
was reported to be 133.11 mg GAE/g of DW. Among hydroxybenzoic acids, the most
abundant was gallic acid, represented by 951.12 μg/g of DW. Also, presence of ellagic,
protocatechuic, p-hydroxybenzoic and syringic acid was observed. Among hydroxycinnamic
acids, chlorogenic acid was the most prevalent, with 0.76 μg/g of DW. With regard to
antioxidant activity and inhibition of LPx, EC50 value for F. pinicola methanol extract was
20.0 μg/mL.
The results of the present study suggest that methanol extract of the wild lignicolus
mushroom F. pinicola acts as natural antioxidant in the prevention of LPx. This mushroom
may be a good source for the development of antioxidant additives.
PB  - МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA
C3  - The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses
T1  - Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6550
ER  - 

@conference{
author = "Kozarski, Maja and Klaus, Anita and Vunduk, Jovana and Lazić, Vesna and Spirović Trifunović, Bojana and Miletić, Srđan and Spasić, Snežana and Jakovljević, Dragica",
year = "2022",
abstract = "Excessive production of reactive oxygen species (ROS) often results in irreversible cell
damage and leads to a number of disorders such as hypertension, heart disease, diabetes,
inflammation, premature aging and cancer. Considering the importance of diet in prevention
of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant
activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural
source of functional food ingredients. Nowadays functional food products based on the
lignicolous mushroom species are increasingly available on the market. Their powders and
extracts are consumed as dietary supplements in the form of capsules or tablets and as
additives in the formulation of healthier food products.
Antioxidant capacity of F. pinicola methanol extract was evaluated in vitro by the inhibition
of lipid peroxidation (LPx) in the linoleic acid model system. Results were normalized and
expressed as EC50 values (μg/mL). The Folin-Ciocalteu reaction adapted for a 96-well
microplate reader was used to determine the total phenol content (TPC) and result was
expressed as mg of gallic acid equivalent (GAE) per g of dry weight (DW) of extract.
Phenolic profile of the extract was analyzed by the liquid chromatography coupled with
tandem mass spectrometry (LC-MS/MS). Based on the analysis, TPC of the methanol extract
was reported to be 133.11 mg GAE/g of DW. Among hydroxybenzoic acids, the most
abundant was gallic acid, represented by 951.12 μg/g of DW. Also, presence of ellagic,
protocatechuic, p-hydroxybenzoic and syringic acid was observed. Among hydroxycinnamic
acids, chlorogenic acid was the most prevalent, with 0.76 μg/g of DW. With regard to
antioxidant activity and inhibition of LPx, EC50 value for F. pinicola methanol extract was
20.0 μg/mL.
The results of the present study suggest that methanol extract of the wild lignicolus
mushroom F. pinicola acts as natural antioxidant in the prevention of LPx. This mushroom
may be a good source for the development of antioxidant additives.",
publisher = "МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA",
journal = "The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses",
title = "Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6550"
}

Kozarski, M., Klaus, A., Vunduk, J., Lazić, V., Spirović Trifunović, B., Miletić, S., Spasić, S.,& Jakovljević, D.. (2022). Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation. in The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses
МАТИЦА СРПСКА, НОВИ САД, СРБИЈА / MATICA SRPSKA, NOVI SAD, SERBIA..
https://hdl.handle.net/21.15107/rcub_agrospace_6550

Kozarski M, Klaus A, Vunduk J, Lazić V, Spirović Trifunović B, Miletić S, Spasić S, Jakovljević D. Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation. in The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses. 2022;.
https://hdl.handle.net/21.15107/rcub_agrospace_6550 .

Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Lazić, Vesna, Spirović Trifunović, Bojana, Miletić, Srđan, Spasić, Snežana, Jakovljević, Dragica, "Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation" in The 7th International Scientific Meeting: Mycology, Mycotoxicology, and Mycoses (2022),
https://hdl.handle.net/21.15107/rcub_agrospace_6550 .

TY  - JOUR
AU  - Stevanović, Snežana M.
AU  - Petrović, Tanja S.
AU  - Marković, Dragan D.
AU  - Milovančević, Uroš M.
AU  - Stevanović, Simo
AU  - Urošević, Tijana
AU  - Kozarski, Maja
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5946
AB  - The effects of different freezing methods and long-term frozen storage on quality of strawberry and raspberry were investigated, that is, the retention of initial property and free radical scavenging (AO) activity after individual quick freezing (IQF) and conventional (discontinuous) freezing. The physicochemical properties, vitamin C, and AO activity were more affected by long-term frozen storage than by freezing itself; however, their better retention was obtained after IQF. Moreover, AO activity was found to be higher in frozen samples compared to fresh fruits, but significant decrease was detected after 8 months of frozen storage. Despite the significantly higher initial content of vitamin C in strawberry, it demonstrated a lower AO activity than raspberry. Sensory attributes were significantly affected by freezing; the most considerable changes were recorded on their texture/firmness, especially after conventional (slow) freezing. Therefore, IQF can generally be suggested as a more suitable method of preserving these delicate fruits than slow freezing. Practical applications: Nowadays, consumers are increasingly interested in foods that possess not only good nutritional value but also health-promoting property. Berries are known as rich sources of micronutrients and antioxidant compounds, but consumption of fresh strawberry and raspberry fruits may be limited because of seasonal and market availability; therefore, they must be preserved commonly by freezing. Frozen food industry is of crucial importance for developing countries, in which the fast freezing industry is rapidly developed. Investigation of the effect of industrial freezing and frozen storage on retention of the quality-related property and antioxidant activity is important for providing the best quality for consumers and health benefits, as well as international fruit trade. In addition, the results obtained in this study could provide best practice and guidance for the frozen fruit industry.
PB  - John Wiley and Sons Inc
T2  - Journal of Food Processing and Preservation
T1  - Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions
DO  - 10.1111/jfpp.15981
ER  - 

@article{
author = "Stevanović, Snežana M. and Petrović, Tanja S. and Marković, Dragan D. and Milovančević, Uroš M. and Stevanović, Simo and Urošević, Tijana and Kozarski, Maja",
year = "2021",
abstract = "The effects of different freezing methods and long-term frozen storage on quality of strawberry and raspberry were investigated, that is, the retention of initial property and free radical scavenging (AO) activity after individual quick freezing (IQF) and conventional (discontinuous) freezing. The physicochemical properties, vitamin C, and AO activity were more affected by long-term frozen storage than by freezing itself; however, their better retention was obtained after IQF. Moreover, AO activity was found to be higher in frozen samples compared to fresh fruits, but significant decrease was detected after 8 months of frozen storage. Despite the significantly higher initial content of vitamin C in strawberry, it demonstrated a lower AO activity than raspberry. Sensory attributes were significantly affected by freezing; the most considerable changes were recorded on their texture/firmness, especially after conventional (slow) freezing. Therefore, IQF can generally be suggested as a more suitable method of preserving these delicate fruits than slow freezing. Practical applications: Nowadays, consumers are increasingly interested in foods that possess not only good nutritional value but also health-promoting property. Berries are known as rich sources of micronutrients and antioxidant compounds, but consumption of fresh strawberry and raspberry fruits may be limited because of seasonal and market availability; therefore, they must be preserved commonly by freezing. Frozen food industry is of crucial importance for developing countries, in which the fast freezing industry is rapidly developed. Investigation of the effect of industrial freezing and frozen storage on retention of the quality-related property and antioxidant activity is important for providing the best quality for consumers and health benefits, as well as international fruit trade. In addition, the results obtained in this study could provide best practice and guidance for the frozen fruit industry.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Food Processing and Preservation",
title = "Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions",
doi = "10.1111/jfpp.15981"
}

Stevanović, S. M., Petrović, T. S., Marković, D. D., Milovančević, U. M., Stevanović, S., Urošević, T.,& Kozarski, M.. (2021). Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions. in Journal of Food Processing and Preservation
John Wiley and Sons Inc..
https://doi.org/10.1111/jfpp.15981

Stevanović SM, Petrović TS, Marković DD, Milovančević UM, Stevanović S, Urošević T, Kozarski M. Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions. in Journal of Food Processing and Preservation. 2021;.
doi:10.1111/jfpp.15981 .

Stevanović, Snežana M., Petrović, Tanja S., Marković, Dragan D., Milovančević, Uroš M., Stevanović, Simo, Urošević, Tijana, Kozarski, Maja, "Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions" in Journal of Food Processing and Preservation (2021),
https://doi.org/10.1111/jfpp.15981 . .

TY  - CONF
AU  - Kozarski, Maja S.
AU  - Klaus, Anita S.
AU  - Lazić, Vesna V.
AU  - Stevanović, Snežana M.
AU  - Jakovljević, Dragica M.
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6515
AB  - Phellinus linteus is a popular medicinal mushroom that is widely used in Asian countries. A number
of studies have confirmed that P. linteus possesses exceptional biological potential useful for
pharmacological applications, including anticancer and anti-inflammatory activities, as well as
antidiabetic, hepatoprotective, and neuroprotective effects. The objective of the present study was to
evaluate antioxidant and immunomodulatory activities of hot water polysaccharide extract obtained
from the medicinal mushroom Phellinus linteus (Berk. et Curt.) Teng. FT-IR was used to study the
polysaccharide profile of the extract. Its antioxidant potential was measured by the conjugated
diene method in the linoleic acid model system. Immunomodulation was tested in vitro by
measuring the synthesis of interferon-gamma (IFN-γ) in healthy human peripheral blood
mononuclear cells (PBMCs) using enzyme linked immunosorbent assay (ELISA). The FT-IR
spectrum of P. linteus hot water extracted polysaccharides showed a typical carbohydrate pattern. A
small amount of proteins was also observed with characteristic absorptions at 1635, 1540 and 1412
cm-1
. Measurements of antioxidant properties in linoleic acid model system revealed relatively high
antioxidant activity with EC50 value of 7.11 mg/mL. After 48 h of P. linteus polysaccharide extract
incubation, the IFN-γ titer displayed immunosuppressive effect, 32.6 pg/mL. The IFN-γ titer for the
suspension of PBMCs in PBS, which was used as a positive control, was found to be 135.2 pg/mL.
Differences in IFN- γ contents in P. linteus extract vs. model control were strongly significant (p <
0.05). The results of this study suggest that the polysaccharide extract of P. linteus acts as a natural
antioxidant and possesses immunomodulatory properties. Therefore, it can be a suitable raw
material for the development of antioxidant food additives. In addition, due to the possible
immunosuppressive effect P. linteus polysaccharide extract is particularly interesting and could find
application in suppression of autoimmune diseases such as rheumatoid arthritis.
C3  - 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online
T1  - ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus
DO  - 10.1016/j.foodchem.2011.06.029
ER  - 

@conference{
author = "Kozarski, Maja S. and Klaus, Anita S. and Lazić, Vesna V. and Stevanović, Snežana M. and Jakovljević, Dragica M.",
year = "2021",
abstract = "Phellinus linteus is a popular medicinal mushroom that is widely used in Asian countries. A number
of studies have confirmed that P. linteus possesses exceptional biological potential useful for
pharmacological applications, including anticancer and anti-inflammatory activities, as well as
antidiabetic, hepatoprotective, and neuroprotective effects. The objective of the present study was to
evaluate antioxidant and immunomodulatory activities of hot water polysaccharide extract obtained
from the medicinal mushroom Phellinus linteus (Berk. et Curt.) Teng. FT-IR was used to study the
polysaccharide profile of the extract. Its antioxidant potential was measured by the conjugated
diene method in the linoleic acid model system. Immunomodulation was tested in vitro by
measuring the synthesis of interferon-gamma (IFN-γ) in healthy human peripheral blood
mononuclear cells (PBMCs) using enzyme linked immunosorbent assay (ELISA). The FT-IR
spectrum of P. linteus hot water extracted polysaccharides showed a typical carbohydrate pattern. A
small amount of proteins was also observed with characteristic absorptions at 1635, 1540 and 1412
cm-1
. Measurements of antioxidant properties in linoleic acid model system revealed relatively high
antioxidant activity with EC50 value of 7.11 mg/mL. After 48 h of P. linteus polysaccharide extract
incubation, the IFN-γ titer displayed immunosuppressive effect, 32.6 pg/mL. The IFN-γ titer for the
suspension of PBMCs in PBS, which was used as a positive control, was found to be 135.2 pg/mL.
Differences in IFN- γ contents in P. linteus extract vs. model control were strongly significant (p <
0.05). The results of this study suggest that the polysaccharide extract of P. linteus acts as a natural
antioxidant and possesses immunomodulatory properties. Therefore, it can be a suitable raw
material for the development of antioxidant food additives. In addition, due to the possible
immunosuppressive effect P. linteus polysaccharide extract is particularly interesting and could find
application in suppression of autoimmune diseases such as rheumatoid arthritis.",
journal = "2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online",
title = "ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus",
doi = "10.1016/j.foodchem.2011.06.029"
}

Kozarski, M. S., Klaus, A. S., Lazić, V. V., Stevanović, S. M.,& Jakovljević, D. M.. (2021). ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus. in 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online.
https://doi.org/10.1016/j.foodchem.2011.06.029

Kozarski MS, Klaus AS, Lazić VV, Stevanović SM, Jakovljević DM. ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus. in 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online. 2021;.
doi:10.1016/j.foodchem.2011.06.029 .

Kozarski, Maja S., Klaus, Anita S., Lazić, Vesna V., Stevanović, Snežana M., Jakovljević, Dragica M., "ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus" in 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online (2021),
https://doi.org/10.1016/j.foodchem.2011.06.029 . .

TY  - CONF
AU  - Lazić, Vesna
AU  - Kozarski, Maja
AU  - Vunduk, Jovana
AU  - Doroški, Ana
AU  - Klaus, Anita
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6523
AB  - Inonotus obliquus (Čaga) je medicinski značajna glјiva čija micelija je tamne boje, a razvija se u kori breze. Pripada
porodici Hymenochaetaceae. Ekstrakti I. obliquus koriste se širom Severne i Istočne Evrope, Koreje i Rusije jer se pretpostavlјa
da pokazuju anitimikrobne, antioksidativne, antiinflamatorne i antitumorske aktivnosti. Hemijski testovi pokazuju
da I. obliquus proizvodi širok spektar sekundarnih metabolita, uklјučujući fenolna jedinjenja, melanine, uglјene
hidrate i proteine.
Zadatak ovog istraživanja je bio da se utvrdi hemijski sastav, kao i antimikrobna i antiadhezivna i antibiofilmska sposobnost
dobijenih subkritičnih vodenih ekstrakata I. obliquus poreklom iz Mongolije (IM) i sa planine Vlasina, Srbija (IS).
Utvrđen je ukupan sadržaj proteina, uglјenih hidrata i fenola, a visok sadržaj fenola u oba ekstrakta. Fenolne kiseline su
identifikovane hemijskom analizom HPLC i potvrđeno je prisustvo hlorogenske kiseline u najvećoj koncentraciji, katehina,
p-kumarinske kiseline i cimetne kiseline.
Antimikrobni potencijal subkritičnih vodenih ekstrakata ispitan je mikrodilucionom metodom. Antiadhezivna i antibiofilmska
sposobnost praćena je korišćenjem Gram pozitivnih bakterija Listeria monocitogenes, Enterococcus faecalis i
Staphilococcus aureus, kao i Gram negativnih Salmonella enteritidis, Escherichia coli i Pseudomonas aeruginosa. Najznačajnija
proizvodnja biofilma zabeležena je kod E. faecalis (treća kategorija) i S. aureus (druga kategorija). Uočeno je
da su ekstrakti IS i IM doveli do 90% adhezije kod E. faecalis i S. aureus kao i do redukcije biofilma, odnosno da je došlo
do smanje sposobnost ove dve bakterije da formiraju biofilm. Gram pozitivne bakterije su se pokazale kao osetlјivije
u prisustvu oba testirana subkritična vodena ekstrakta (MBC - 20 mg/mL u svim eksperimentima). Prema dobijenim
vrednostima MIC, IM (5 mg/mL, 2,5 mg/mL, 2,5 mg/mL) je ispolјio jači efekat od IS (10mg/mL, 5 mg/mL, 5 mg/mL) na L.
monocitogenes, E. faecalis i S. aureus. Ova istraživanja pokazala su da su testirani subkritični vodeni ekstrakti I. obliquus
veoma efikasni u sprečavanju nastanka biofilma, posebno onih odabranih Gram-pozitivnih bakterija. Ovo otkriće bi moglo
biti veoma korisno u borbi protiv biofilma, uzimajući u obzir sve probleme i opasnosti koje oni nose.
PB  - Udruženje mikrobiologa Srbije
C3  - Nove i ponovo aktuelne mikrobne infekcije - Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE
T1  - UTICAJ EKSTRAKATA GLJIVE INONOTUS OBLIQUUS NA FORMIRANJE BIOFILMA PATOGENIH BAKTERIJA
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6523
ER  - 

@conference{
author = "Lazić, Vesna and Kozarski, Maja and Vunduk, Jovana and Doroški, Ana and Klaus, Anita",
year = "2021",
abstract = "Inonotus obliquus (Čaga) je medicinski značajna glјiva čija micelija je tamne boje, a razvija se u kori breze. Pripada
porodici Hymenochaetaceae. Ekstrakti I. obliquus koriste se širom Severne i Istočne Evrope, Koreje i Rusije jer se pretpostavlјa
da pokazuju anitimikrobne, antioksidativne, antiinflamatorne i antitumorske aktivnosti. Hemijski testovi pokazuju
da I. obliquus proizvodi širok spektar sekundarnih metabolita, uklјučujući fenolna jedinjenja, melanine, uglјene
hidrate i proteine.
Zadatak ovog istraživanja je bio da se utvrdi hemijski sastav, kao i antimikrobna i antiadhezivna i antibiofilmska sposobnost
dobijenih subkritičnih vodenih ekstrakata I. obliquus poreklom iz Mongolije (IM) i sa planine Vlasina, Srbija (IS).
Utvrđen je ukupan sadržaj proteina, uglјenih hidrata i fenola, a visok sadržaj fenola u oba ekstrakta. Fenolne kiseline su
identifikovane hemijskom analizom HPLC i potvrđeno je prisustvo hlorogenske kiseline u najvećoj koncentraciji, katehina,
p-kumarinske kiseline i cimetne kiseline.
Antimikrobni potencijal subkritičnih vodenih ekstrakata ispitan je mikrodilucionom metodom. Antiadhezivna i antibiofilmska
sposobnost praćena je korišćenjem Gram pozitivnih bakterija Listeria monocitogenes, Enterococcus faecalis i
Staphilococcus aureus, kao i Gram negativnih Salmonella enteritidis, Escherichia coli i Pseudomonas aeruginosa. Najznačajnija
proizvodnja biofilma zabeležena je kod E. faecalis (treća kategorija) i S. aureus (druga kategorija). Uočeno je
da su ekstrakti IS i IM doveli do 90% adhezije kod E. faecalis i S. aureus kao i do redukcije biofilma, odnosno da je došlo
do smanje sposobnost ove dve bakterije da formiraju biofilm. Gram pozitivne bakterije su se pokazale kao osetlјivije
u prisustvu oba testirana subkritična vodena ekstrakta (MBC - 20 mg/mL u svim eksperimentima). Prema dobijenim
vrednostima MIC, IM (5 mg/mL, 2,5 mg/mL, 2,5 mg/mL) je ispolјio jači efekat od IS (10mg/mL, 5 mg/mL, 5 mg/mL) na L.
monocitogenes, E. faecalis i S. aureus. Ova istraživanja pokazala su da su testirani subkritični vodeni ekstrakti I. obliquus
veoma efikasni u sprečavanju nastanka biofilma, posebno onih odabranih Gram-pozitivnih bakterija. Ovo otkriće bi moglo
biti veoma korisno u borbi protiv biofilma, uzimajući u obzir sve probleme i opasnosti koje oni nose.",
publisher = "Udruženje mikrobiologa Srbije",
journal = "Nove i ponovo aktuelne mikrobne infekcije - Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE",
title = "UTICAJ EKSTRAKATA GLJIVE INONOTUS OBLIQUUS NA FORMIRANJE BIOFILMA PATOGENIH BAKTERIJA",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6523"
}

Lazić, V., Kozarski, M., Vunduk, J., Doroški, A.,& Klaus, A.. (2021). UTICAJ EKSTRAKATA GLJIVE INONOTUS OBLIQUUS NA FORMIRANJE BIOFILMA PATOGENIH BAKTERIJA. in Nove i ponovo aktuelne mikrobne infekcije - Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE
Udruženje mikrobiologa Srbije..
https://hdl.handle.net/21.15107/rcub_agrospace_6523

Lazić V, Kozarski M, Vunduk J, Doroški A, Klaus A. UTICAJ EKSTRAKATA GLJIVE INONOTUS OBLIQUUS NA FORMIRANJE BIOFILMA PATOGENIH BAKTERIJA. in Nove i ponovo aktuelne mikrobne infekcije - Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6523 .

Lazić, Vesna, Kozarski, Maja, Vunduk, Jovana, Doroški, Ana, Klaus, Anita, "UTICAJ EKSTRAKATA GLJIVE INONOTUS OBLIQUUS NA FORMIRANJE BIOFILMA PATOGENIH BAKTERIJA" in Nove i ponovo aktuelne mikrobne infekcije - Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6523 .

TY  - JOUR
AU  - Vunduk, Jovana
AU  - Kozarski, Maja
AU  - Đekić, Ilija
AU  - Tomašević, Igor
AU  - Klaus, Anita
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5803
AB  - Mushrooms are proven as a functional food due to their numerous beneficial effects on human health. Contemporary consumers
purchase cultivated mushrooms that spend some time on the shelf, which makes it questionable whether this food can still
be called “functional”. An examination was performed on selected characteristics of white and brown (Portobello) Agaricus
bisporus stored in commercial (air) and three modified atmosphere packagings (MAP): high nitrogen, low carbon dioxide
and low oxygen packaging. The amount of ascorbic acid decreased throughout 22 days for both varieties, especially in the
white variety stored in commercial packaging. Similarly, total flavonoids decreased, although not to a significant degree.
Metal chelating ability was pronounced throughout the storage period, with minor changes in the case of the brown variety.
Diabetes-connected enzymes were inhibited by A. bisporus, while inhibition was significantly higher toward α-amylase.
Nitrogen-rich packaging suppressed α-amylase and stimulated α-glucosidase in the white strain. Both the commercial packaging
and the MAP samples exhibited changes in their functional characteristics over three weeks of cold storage. MAP,
especially the low oxygen packaging, provided the best option for the preservation of the majority of functional characteristics
examined in this research. Enzymes activities appeared to be more specifically tuned, and dependent on parameters
not covered here. Brown variety was more resistant to environmental changes with respect to its functional characteristics.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - European Food Research and Technology
T1  - Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus
IS  - 3
VL  - 247
DO  - 10.1007/s00217-020-03666-x
ER  - 

@article{
author = "Vunduk, Jovana and Kozarski, Maja and Đekić, Ilija and Tomašević, Igor and Klaus, Anita",
year = "2021",
abstract = "Mushrooms are proven as a functional food due to their numerous beneficial effects on human health. Contemporary consumers
purchase cultivated mushrooms that spend some time on the shelf, which makes it questionable whether this food can still
be called “functional”. An examination was performed on selected characteristics of white and brown (Portobello) Agaricus
bisporus stored in commercial (air) and three modified atmosphere packagings (MAP): high nitrogen, low carbon dioxide
and low oxygen packaging. The amount of ascorbic acid decreased throughout 22 days for both varieties, especially in the
white variety stored in commercial packaging. Similarly, total flavonoids decreased, although not to a significant degree.
Metal chelating ability was pronounced throughout the storage period, with minor changes in the case of the brown variety.
Diabetes-connected enzymes were inhibited by A. bisporus, while inhibition was significantly higher toward α-amylase.
Nitrogen-rich packaging suppressed α-amylase and stimulated α-glucosidase in the white strain. Both the commercial packaging
and the MAP samples exhibited changes in their functional characteristics over three weeks of cold storage. MAP,
especially the low oxygen packaging, provided the best option for the preservation of the majority of functional characteristics
examined in this research. Enzymes activities appeared to be more specifically tuned, and dependent on parameters
not covered here. Brown variety was more resistant to environmental changes with respect to its functional characteristics.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "European Food Research and Technology",
title = "Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus",
number = "3",
volume = "247",
doi = "10.1007/s00217-020-03666-x"
}

Vunduk, J., Kozarski, M., Đekić, I., Tomašević, I.,& Klaus, A.. (2021). Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus. in European Food Research and Technology
Springer Science and Business Media Deutschland GmbH., 247(3).
https://doi.org/10.1007/s00217-020-03666-x

Vunduk J, Kozarski M, Đekić I, Tomašević I, Klaus A. Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus. in European Food Research and Technology. 2021;247(3).
doi:10.1007/s00217-020-03666-x .

Vunduk, Jovana, Kozarski, Maja, Đekić, Ilija, Tomašević, Igor, Klaus, Anita, "Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus" in European Food Research and Technology, 247, no. 3 (2021),
https://doi.org/10.1007/s00217-020-03666-x . .

TY  - JOUR
AU  - Doroški, Ana
AU  - Klaus, Anita
AU  - Kozarski, Maja
AU  - Nikolić, Biljana
AU  - Vunduk, Jovana
AU  - Lazić, Vesna
AU  - Đekić, Ilija
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6023
AB  - The objective of this study was to develop a single quality index of chemical characteristics of
Pleurotus ostreatus extracts on 7th and 14th day of its shelf life, derived from the mushroom fruiting
bodies. P. ostreatus was cultivated on four substrates containing different ratio of wine industry
waste-grape pomace (P) and wheat straw (S): 100P, 80P20S, 50P50S, 20P80S. Four quality parameters of P. ostreatus mushroom extracts, i.e. antioxidative parameters: ABTS+ and DPPH• free radical
scavenging capability, total phenolic compounds (TPC) and total polysaccharides (TPS) were used to
define the final extract quality index. Analysis indicated 100P and 80P20S as the samples cultivated
on the substrate with higher percent of grape pomace, as the best quality at the 7th day of its shelf life.
On the other hand, final quality score indicated 50P50S and 20P80S, cultivated on a substrate with a
lower percent of grape pomace, as the best quality samples at the 14th day of its shelf life. According
to the results, samples cultivated on a higher pomace content substrate are of better quality in a shorter storage time period.
PB  - Faculty of Technology, Novi Sad
T2  - Acta Periodica Technologica
T1  - Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties
EP  - 32
IS  - 52
SP  - 25
DO  - 10.2298/APT2152025D
ER  - 

@article{
author = "Doroški, Ana and Klaus, Anita and Kozarski, Maja and Nikolić, Biljana and Vunduk, Jovana and Lazić, Vesna and Đekić, Ilija",
year = "2021",
abstract = "The objective of this study was to develop a single quality index of chemical characteristics of
Pleurotus ostreatus extracts on 7th and 14th day of its shelf life, derived from the mushroom fruiting
bodies. P. ostreatus was cultivated on four substrates containing different ratio of wine industry
waste-grape pomace (P) and wheat straw (S): 100P, 80P20S, 50P50S, 20P80S. Four quality parameters of P. ostreatus mushroom extracts, i.e. antioxidative parameters: ABTS+ and DPPH• free radical
scavenging capability, total phenolic compounds (TPC) and total polysaccharides (TPS) were used to
define the final extract quality index. Analysis indicated 100P and 80P20S as the samples cultivated
on the substrate with higher percent of grape pomace, as the best quality at the 7th day of its shelf life.
On the other hand, final quality score indicated 50P50S and 20P80S, cultivated on a substrate with a
lower percent of grape pomace, as the best quality samples at the 14th day of its shelf life. According
to the results, samples cultivated on a higher pomace content substrate are of better quality in a shorter storage time period.",
publisher = "Faculty of Technology, Novi Sad",
journal = "Acta Periodica Technologica",
title = "Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties",
pages = "32-25",
number = "52",
doi = "10.2298/APT2152025D"
}

Doroški, A., Klaus, A., Kozarski, M., Nikolić, B., Vunduk, J., Lazić, V.,& Đekić, I.. (2021). Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties. in Acta Periodica Technologica
Faculty of Technology, Novi Sad.(52), 25-32.
https://doi.org/10.2298/APT2152025D

Doroški A, Klaus A, Kozarski M, Nikolić B, Vunduk J, Lazić V, Đekić I. Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties. in Acta Periodica Technologica. 2021;(52):25-32.
doi:10.2298/APT2152025D .

Doroški, Ana, Klaus, Anita, Kozarski, Maja, Nikolić, Biljana, Vunduk, Jovana, Lazić, Vesna, Đekić, Ilija, "Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties" in Acta Periodica Technologica, no. 52 (2021):25-32,
https://doi.org/10.2298/APT2152025D . .

TY  - CONF
AU  - Lazić, Vesna
AU  - Kozarski, Maja
AU  - Vunduk, Jovana
AU  - Doroški, Ana
AU  - Klaus, Anita
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6516
AB  - Inonotus obliquus (Čaga) je medicinski značajna glјiva čija micelija je tamne boje, a razvija se u kori breze. Pripada porodici Hymenochaetaceae. Ekstrakti I. obliquus koriste se širom Severne i Istočne Evrope, Koreje i Rusije jer se pretpostavlјa da pokazuju anitimikrobne, antioksidativne, antiinflamatorne i antitumorske aktivnosti. Hemijski testovi pokazuju da I. obliquus proizvodi širok spektar sekundarnih metabolita, uklјučujući fenolna jedinjenja, melanine, uglјene hidrate i proteine.
 Zadatak ovog istraživanja je bio da se utvrdi hemijski sastav, kao i antimikrobna i antiadhezivna i antibiofilmska sposobnost dobijenih subkritičnih vodenih ekstrakata I. obliquus poreklom iz Mongolije (IM) i sa planine Vlasina, Srbija (IS). Utvrđen je ukupan sadržaj proteina, uglјenih hidrata i fenola, a visok sadržaj fenola u oba ekstrakta. Fenolne kiseline su identifikovane hemijskom analizom HPLC i potvrđeno je prisustvo hlorogenske kiseline u najvećoj koncentraciji, katehina, p-kumarinske kiseline i cimetne kiseline.
Antimikrobni potencijal subkritičnih vodenih ekstrakata ispitan je mikrodilucionom metodom. Antiadhezivna i antibiofilmska sposobnost praćena je korišćenjem Gram pozitivnih bakterija Listeria monocitogenes, Enterococcus faecalis i Staphilococcus aureus, kao i Gram negativnih Salmonella enteritidis, Escherichia coli i Pseudomonas aeruginosa. Najznačajnija proizvodnja biofilma zabeležena je kod E. faecalis (treća kategorija) i S. aureus (druga kategorija). Uočeno je da su ekstrakti IS i IM doveli do 90% adhezije kod E. faecalis i S. aureus kao i do redukcije biofilma, odnosno da je došlo do smanje sposobnost ove dve bakterije da formiraju biofilm. Gram pozitivne bakterije su se pokazale kao osetlјivije u prisustvu oba testirana subkritična vodena ekstrakta (MBC - 20 mg/mL u svim eksperimentima). Prema dobijenim vrednostima MIC, IM (5 mg/mL, 2,5 mg/mL, 2,5 mg/mL) je ispolјio jači efekat od IS (10mg/mL, 5 mg/mL, 5 mg/mL) na L. monocitogenes, E. faecalis i S. aureus. Ova istraživanja pokazala su da su testirani subkritični vodeni ekstrakti I. obliquus veoma efikasni u sprečavanju nastanka biofilma, posebno onih odabranih Gram-pozitivnih bakterija. Ovo otkriće bi moglo biti veoma korisno u borbi protiv biofilma, uzimajući u obzir sve probleme i opasnosti koje oni nose.
PB  - Udruženje mikrobiologa Srbije
C3  - Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE, Udruženje mikrobiologa Srbije
T1  - Uticaj ekstrakata gljive Inonotus obliquus na formiranje biofilma patogenih bakterija
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6516
ER  - 

@conference{
author = "Lazić, Vesna and Kozarski, Maja and Vunduk, Jovana and Doroški, Ana and Klaus, Anita",
year = "2021",
abstract = "Inonotus obliquus (Čaga) je medicinski značajna glјiva čija micelija je tamne boje, a razvija se u kori breze. Pripada porodici Hymenochaetaceae. Ekstrakti I. obliquus koriste se širom Severne i Istočne Evrope, Koreje i Rusije jer se pretpostavlјa da pokazuju anitimikrobne, antioksidativne, antiinflamatorne i antitumorske aktivnosti. Hemijski testovi pokazuju da I. obliquus proizvodi širok spektar sekundarnih metabolita, uklјučujući fenolna jedinjenja, melanine, uglјene hidrate i proteine.
 Zadatak ovog istraživanja je bio da se utvrdi hemijski sastav, kao i antimikrobna i antiadhezivna i antibiofilmska sposobnost dobijenih subkritičnih vodenih ekstrakata I. obliquus poreklom iz Mongolije (IM) i sa planine Vlasina, Srbija (IS). Utvrđen je ukupan sadržaj proteina, uglјenih hidrata i fenola, a visok sadržaj fenola u oba ekstrakta. Fenolne kiseline su identifikovane hemijskom analizom HPLC i potvrđeno je prisustvo hlorogenske kiseline u najvećoj koncentraciji, katehina, p-kumarinske kiseline i cimetne kiseline.
Antimikrobni potencijal subkritičnih vodenih ekstrakata ispitan je mikrodilucionom metodom. Antiadhezivna i antibiofilmska sposobnost praćena je korišćenjem Gram pozitivnih bakterija Listeria monocitogenes, Enterococcus faecalis i Staphilococcus aureus, kao i Gram negativnih Salmonella enteritidis, Escherichia coli i Pseudomonas aeruginosa. Najznačajnija proizvodnja biofilma zabeležena je kod E. faecalis (treća kategorija) i S. aureus (druga kategorija). Uočeno je da su ekstrakti IS i IM doveli do 90% adhezije kod E. faecalis i S. aureus kao i do redukcije biofilma, odnosno da je došlo do smanje sposobnost ove dve bakterije da formiraju biofilm. Gram pozitivne bakterije su se pokazale kao osetlјivije u prisustvu oba testirana subkritična vodena ekstrakta (MBC - 20 mg/mL u svim eksperimentima). Prema dobijenim vrednostima MIC, IM (5 mg/mL, 2,5 mg/mL, 2,5 mg/mL) je ispolјio jači efekat od IS (10mg/mL, 5 mg/mL, 5 mg/mL) na L. monocitogenes, E. faecalis i S. aureus. Ova istraživanja pokazala su da su testirani subkritični vodeni ekstrakti I. obliquus veoma efikasni u sprečavanju nastanka biofilma, posebno onih odabranih Gram-pozitivnih bakterija. Ovo otkriće bi moglo biti veoma korisno u borbi protiv biofilma, uzimajući u obzir sve probleme i opasnosti koje oni nose.",
publisher = "Udruženje mikrobiologa Srbije",
journal = "Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE, Udruženje mikrobiologa Srbije",
title = "Uticaj ekstrakata gljive Inonotus obliquus na formiranje biofilma patogenih bakterija",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6516"
}

Lazić, V., Kozarski, M., Vunduk, J., Doroški, A.,& Klaus, A.. (2021). Uticaj ekstrakata gljive Inonotus obliquus na formiranje biofilma patogenih bakterija. in Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE, Udruženje mikrobiologa Srbije
Udruženje mikrobiologa Srbije..
https://hdl.handle.net/21.15107/rcub_agrospace_6516

Lazić V, Kozarski M, Vunduk J, Doroški A, Klaus A. Uticaj ekstrakata gljive Inonotus obliquus na formiranje biofilma patogenih bakterija. in Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE, Udruženje mikrobiologa Srbije. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_6516 .

Lazić, Vesna, Kozarski, Maja, Vunduk, Jovana, Doroški, Ana, Klaus, Anita, "Uticaj ekstrakata gljive Inonotus obliquus na formiranje biofilma patogenih bakterija" in Simpozijum produkcija biofilma - rizici za nastanak intrahospitalnih infekcija i problemi higijene u proizvodnji i distribuciji hrane - ONLINE, Udruženje mikrobiologa Srbije (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_6516 .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Jakovljević, Dragica
AU  - Jadranin, Milka
AU  - Nikšić, Miomir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5278
AB  - The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal-human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)(-1)), inhibition of LPx (EC50 = 1.07 mg mL(-1)), ACE (IC50 = 0.54 mg mL(-1)) and AChE (IC50 = 0.37 mg mL(-1)), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL(-1)) and K562 (IC50 = 0.11 mg mL(-1)) cells. AbHW contained higher total protein (6.4 g (100 g)(-1)), carbohydrate (75.4 g (100 g)(-1)) and beta-glucan (55.1 g (100 g)(-1)) contents and induced significant proliferation of healthy PBMC from 152-116 % in the concentration range of 0.047-0.187 mg mL(-1). The difference in the biological activity of the extracts provides guidance on their use as functional food.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum - A comparative overview
EP  - 735
IS  - 6
SP  - 721
VL  - 85
DO  - 10.2298/JSC190930129K
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Vunduk, Jovana and Jakovljević, Dragica and Jadranin, Milka and Nikšić, Miomir",
year = "2020",
abstract = "The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal-human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)(-1)), inhibition of LPx (EC50 = 1.07 mg mL(-1)), ACE (IC50 = 0.54 mg mL(-1)) and AChE (IC50 = 0.37 mg mL(-1)), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL(-1)) and K562 (IC50 = 0.11 mg mL(-1)) cells. AbHW contained higher total protein (6.4 g (100 g)(-1)), carbohydrate (75.4 g (100 g)(-1)) and beta-glucan (55.1 g (100 g)(-1)) contents and induced significant proliferation of healthy PBMC from 152-116 % in the concentration range of 0.047-0.187 mg mL(-1). The difference in the biological activity of the extracts provides guidance on their use as functional food.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum - A comparative overview",
pages = "735-721",
number = "6",
volume = "85",
doi = "10.2298/JSC190930129K"
}

Kozarski, M., Klaus, A., Vunduk, J., Jakovljević, D., Jadranin, M.,& Nikšić, M.. (2020). Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum - A comparative overview. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 85(6), 721-735.
https://doi.org/10.2298/JSC190930129K

Kozarski M, Klaus A, Vunduk J, Jakovljević D, Jadranin M, Nikšić M. Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum - A comparative overview. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2020;85(6):721-735.
doi:10.2298/JSC190930129K .

Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Jakovljević, Dragica, Jadranin, Milka, Nikšić, Miomir, "Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum - A comparative overview" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 85, no. 6 (2020):721-735,
https://doi.org/10.2298/JSC190930129K . .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Nikšić, Miomir
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5489
AB  - Oxidative stress is among the main culprits for the progression of chronic diseases such as cardiovascular diseases, diabetes, and neurodegenerative disorders. It is well known that dark chocolate possesses polyphenols as major constituents which dietary consumption has been associated to health beneficial effects. Consequently, this study aimed to analyze dark chocolate (DC) and dark chocolate enriched with medicinal mushroom Coriolus versicolor (DCC) and hazelnuts (DCH) regarding antioxidant potential and total polyphenol (TPC), flavonoid (TFC), flavan-3-ol (FLA) and proanthocyanidin (PCA) content. DPPH•, CUPRAC and ABTS•+ assays were applied for measuring antioxidant capacity. The average of all antioxidant tests for each product was used for calculating the antioxidant potency composite index (ACI). The mean values of all antioxidant assays indicated that all chocolate products contained potent antioxidants. The addition of hazelnuts to dark chocolate significantly affected its total flavonoid content. Regression analysis among results obtained with antioxidant assays revealed that dark chocolate and dark chocolate products may act in mixed mode, by direct reduction via electron transfer or by radical quenching via H atom transfer. Flavan-3ols had the most significant impact on the ability of the analyzed samples to reduce metals, while proanthocyanidins primarily acted as radical scavengers. The obtained results provided additional information regarding the value-added dark chocolates enriched with bioactive compounds of medicinal mushroom and plant origin.
AB  - Oksidativni stres je odgovoran za napredovanje hroničnih oboljenja kao što su kardiovaskularne bolesti, dijabetes i neurodegenerativni poremećaji. Poznato je da crna čokolada sadrži polifenole čiji unos ostvaruje pozitivan uticaj na zdravlje. Shodno tome, cilj ovog istraživanja bio je da se analizira antioksidativni potencijal i ukupni sadržaj polifenola (TPC), flavonoida (TFC), flavan-3-ola (FLA) i proantocijanidina (PCA) crne čokolade (DC) i crne čokolade sa dodatkom gljive Coriolus versicolor (DCC) i lešnika (DCH). Antioksidativni potencijal poređen je primenom DPPH•, CUPRAC i ABTS•+ testova za merenje antioksidativnih sposobnosti in vitro. Srednja vrednost svih antioksidativnih testova za svaki analizirani proizvod korišćena je za izračunavanje antioksidativnog kompozitnog indeksa (ACI). Rezultati istraživanja su pokazali da DC, DCC i DCH imaju izraženi antioksidativni potencijal. Dodavanje lešnika tamnoj čokoladi značajno je uticalo na njen ukupni sadržaj flavonoida. Regresiona analiza rezultata dobijenih antioksidativnim testovima je potvrdila da DC, DCC i DCH ispoljavaju antioksidativni potencijal različitim reakcionim mehanizmima tj. direktnom redukcijom prenošenjem elektrona i hvatanjem slobodnih radikala prenošenjem vodonikovih atoma. Potvrđeno je da flavan-3-oli imaju značajan uticaj na sposobnost analiziranih uzoraka da redukuju metale, dok proantocijanidini uglavnom deluju kao hvatači slobodnih radikala. Dobijeni rezultati ovog istraživanja pružaju dodatne informacije o biološkom potencijalu crne čokolade sa dodatkom bioaktivnih komponenata biljnog porekla i lekovitih gljiva.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate
T1  - Uticaj dodatka lekovite gljive Coriolus versicolor i lešnika na antioksidativni potencijal i bioaktivni sadržaj crne čokolade
EP  - 32
IS  - 1
SP  - 23
VL  - 47
DO  - 10.5937/FFR2001023K
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Vunduk, Jovana and Nikšić, Miomir",
year = "2020",
abstract = "Oxidative stress is among the main culprits for the progression of chronic diseases such as cardiovascular diseases, diabetes, and neurodegenerative disorders. It is well known that dark chocolate possesses polyphenols as major constituents which dietary consumption has been associated to health beneficial effects. Consequently, this study aimed to analyze dark chocolate (DC) and dark chocolate enriched with medicinal mushroom Coriolus versicolor (DCC) and hazelnuts (DCH) regarding antioxidant potential and total polyphenol (TPC), flavonoid (TFC), flavan-3-ol (FLA) and proanthocyanidin (PCA) content. DPPH•, CUPRAC and ABTS•+ assays were applied for measuring antioxidant capacity. The average of all antioxidant tests for each product was used for calculating the antioxidant potency composite index (ACI). The mean values of all antioxidant assays indicated that all chocolate products contained potent antioxidants. The addition of hazelnuts to dark chocolate significantly affected its total flavonoid content. Regression analysis among results obtained with antioxidant assays revealed that dark chocolate and dark chocolate products may act in mixed mode, by direct reduction via electron transfer or by radical quenching via H atom transfer. Flavan-3ols had the most significant impact on the ability of the analyzed samples to reduce metals, while proanthocyanidins primarily acted as radical scavengers. The obtained results provided additional information regarding the value-added dark chocolates enriched with bioactive compounds of medicinal mushroom and plant origin., Oksidativni stres je odgovoran za napredovanje hroničnih oboljenja kao što su kardiovaskularne bolesti, dijabetes i neurodegenerativni poremećaji. Poznato je da crna čokolada sadrži polifenole čiji unos ostvaruje pozitivan uticaj na zdravlje. Shodno tome, cilj ovog istraživanja bio je da se analizira antioksidativni potencijal i ukupni sadržaj polifenola (TPC), flavonoida (TFC), flavan-3-ola (FLA) i proantocijanidina (PCA) crne čokolade (DC) i crne čokolade sa dodatkom gljive Coriolus versicolor (DCC) i lešnika (DCH). Antioksidativni potencijal poređen je primenom DPPH•, CUPRAC i ABTS•+ testova za merenje antioksidativnih sposobnosti in vitro. Srednja vrednost svih antioksidativnih testova za svaki analizirani proizvod korišćena je za izračunavanje antioksidativnog kompozitnog indeksa (ACI). Rezultati istraživanja su pokazali da DC, DCC i DCH imaju izraženi antioksidativni potencijal. Dodavanje lešnika tamnoj čokoladi značajno je uticalo na njen ukupni sadržaj flavonoida. Regresiona analiza rezultata dobijenih antioksidativnim testovima je potvrdila da DC, DCC i DCH ispoljavaju antioksidativni potencijal različitim reakcionim mehanizmima tj. direktnom redukcijom prenošenjem elektrona i hvatanjem slobodnih radikala prenošenjem vodonikovih atoma. Potvrđeno je da flavan-3-oli imaju značajan uticaj na sposobnost analiziranih uzoraka da redukuju metale, dok proantocijanidini uglavnom deluju kao hvatači slobodnih radikala. Dobijeni rezultati ovog istraživanja pružaju dodatne informacije o biološkom potencijalu crne čokolade sa dodatkom bioaktivnih komponenata biljnog porekla i lekovitih gljiva.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate, Uticaj dodatka lekovite gljive Coriolus versicolor i lešnika na antioksidativni potencijal i bioaktivni sadržaj crne čokolade",
pages = "32-23",
number = "1",
volume = "47",
doi = "10.5937/FFR2001023K"
}

Kozarski, M., Klaus, A., Vunduk, J.,& Nikšić, M.. (2020). The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 47(1), 23-32.
https://doi.org/10.5937/FFR2001023K

Kozarski M, Klaus A, Vunduk J, Nikšić M. The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate. in Food and Feed Research. 2020;47(1):23-32.
doi:10.5937/FFR2001023K .

Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Nikšić, Miomir, "The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate" in Food and Feed Research, 47, no. 1 (2020):23-32,
https://doi.org/10.5937/FFR2001023K . .

TY  - JOUR
AU  - Kozarski, M.
AU  - Lazić, V.
AU  - Pantić, M.
AU  - Topalović, K.
AU  - Nikšič, M.
AU  - Klaus, A.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6542
AB  - Agaricus bisporus also known as champignon or white button mushroom was
cultivated on digested organic waste. Its aqueous extract was investigated for the
antioxidant potential, which could strengthen the protection of organism against
oxidative stress damages. Likewise, acetylcholinesterase (AChE) inhibitory activity
was observed. The crucial role of AChE in neural transmission makes it a primary
target of a large number of cholinesterase-inhibiting drugs involved in the
prevention of neurodegenerative diseases e.g. Alzheimer’s disease (AD). The
antioxidant activities were evaluated by in vitro models including, 1,1-diphenyl-2-
picrylhydrazyl (DPPH) free radical scavenging activity, prevention of lipid
peroxidation (LPx) in a linoleic acid model system and chelating ability on ferrous
ions. Based on the analysis, hot water extracts showed a typical carbohydrate
pattern with the presence of polyphenols and small amounts of proteins. With
regard to scavenging ability on DPPH radicals, the EC50 value for A. bisporus was
0.89 mg/ml, the EC50 value of the chelating abilities on ferrous ions was 1.38
mg/ml and for inhibition of LPx EC50 value was found to be 1.77 mg/ml. Extract of
A. bisporus had a noticeable inhibition towards AChE, IC50=1.02 mg/ml and
exerted weak toxicity against healthy human peripheral blood mononuclear cells
(PBMC). More than 60% of cell viability was observed at 2 mg/ml. The good
antioxidative properties and AChE inhibitory activity of A. bisporus aqueous
extract make it suitable for everyday use as an inexpensive dietary supplement.
PB  - University of Banja Luka
T2  - International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska
T1  - POTENTIAL OF CHAMPIGNONS CULTIVATED ON DIGESTED ORGANIC WASTE IN THE PREVENTION AGAINST OXIDATIVE STRESS AND NEURAL DAMAGE
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6542
ER  - 

@article{
author = "Kozarski, M. and Lazić, V. and Pantić, M. and Topalović, K. and Nikšič, M. and Klaus, A.",
year = "2020",
abstract = "Agaricus bisporus also known as champignon or white button mushroom was
cultivated on digested organic waste. Its aqueous extract was investigated for the
antioxidant potential, which could strengthen the protection of organism against
oxidative stress damages. Likewise, acetylcholinesterase (AChE) inhibitory activity
was observed. The crucial role of AChE in neural transmission makes it a primary
target of a large number of cholinesterase-inhibiting drugs involved in the
prevention of neurodegenerative diseases e.g. Alzheimer’s disease (AD). The
antioxidant activities were evaluated by in vitro models including, 1,1-diphenyl-2-
picrylhydrazyl (DPPH) free radical scavenging activity, prevention of lipid
peroxidation (LPx) in a linoleic acid model system and chelating ability on ferrous
ions. Based on the analysis, hot water extracts showed a typical carbohydrate
pattern with the presence of polyphenols and small amounts of proteins. With
regard to scavenging ability on DPPH radicals, the EC50 value for A. bisporus was
0.89 mg/ml, the EC50 value of the chelating abilities on ferrous ions was 1.38
mg/ml and for inhibition of LPx EC50 value was found to be 1.77 mg/ml. Extract of
A. bisporus had a noticeable inhibition towards AChE, IC50=1.02 mg/ml and
exerted weak toxicity against healthy human peripheral blood mononuclear cells
(PBMC). More than 60% of cell viability was observed at 2 mg/ml. The good
antioxidative properties and AChE inhibitory activity of A. bisporus aqueous
extract make it suitable for everyday use as an inexpensive dietary supplement.",
publisher = "University of Banja Luka",
journal = "International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska",
title = "POTENTIAL OF CHAMPIGNONS CULTIVATED ON DIGESTED ORGANIC WASTE IN THE PREVENTION AGAINST OXIDATIVE STRESS AND NEURAL DAMAGE",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6542"
}

Kozarski, M., Lazić, V., Pantić, M., Topalović, K., Nikšič, M.,& Klaus, A.. (2020). POTENTIAL OF CHAMPIGNONS CULTIVATED ON DIGESTED ORGANIC WASTE IN THE PREVENTION AGAINST OXIDATIVE STRESS AND NEURAL DAMAGE. in International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska
University of Banja Luka..
https://hdl.handle.net/21.15107/rcub_agrospace_6542

Kozarski M, Lazić V, Pantić M, Topalović K, Nikšič M, Klaus A. POTENTIAL OF CHAMPIGNONS CULTIVATED ON DIGESTED ORGANIC WASTE IN THE PREVENTION AGAINST OXIDATIVE STRESS AND NEURAL DAMAGE. in International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska. 2020;.
https://hdl.handle.net/21.15107/rcub_agrospace_6542 .

Kozarski, M., Lazić, V., Pantić, M., Topalović, K., Nikšič, M., Klaus, A., "POTENTIAL OF CHAMPIGNONS CULTIVATED ON DIGESTED ORGANIC WASTE IN THE PREVENTION AGAINST OXIDATIVE STRESS AND NEURAL DAMAGE" in International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska (2020),
https://hdl.handle.net/21.15107/rcub_agrospace_6542 .

TY  - JOUR
AU  - Doroški, Ana
AU  - Klaus, Anita
AU  - Kozarski, Maja
AU  - Cvetković, Stefana
AU  - Nikolić, Biljana
AU  - Jakovljević, Dragica
AU  - Tomasevic, Igor
AU  - Vunduk, Jovana
AU  - Lazić, Vesna
AU  - Djekic, Ilija
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6506
AB  - Total quality index approach was used to reflect the quality of Pleurotus ostreatus
mushroom, cultivated on grape pomace (P) as food waste substrate and wheat straw
(S), in four different combined ratios: 100P; 80P20S; 50P50S; and 20P80S, with
100S, serving as control substrate. This approach involved two directions of mushroom
analysis. The whole mushroom fruiting bodies analysis introduced a single total
quality index (TQIM) based on 11 quality parameters. The mushroom water extracts
analysis was presented by another single total quality index (TQIE), with seven quality
parameters. This mathematical model revealed that the mushroom extracts have a
better overall quality score with higher pomace content in substrate mixture, compared
to the whole mushroom quality changes throughout the observed shelf life
period. Future research may pay attention to the phytotoxicity of grape pomace as a
food waste, as well as the influence of grape pomace on glucan content, as a healthbeneficial
compounds.
PB  - Wiley
T2  - Journal of Food Processing and Preservation
T1  - The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus—Total quality index approach
VL  - 00:e15096
DO  - 10.1111/jfpp.15096
ER  - 

@article{
author = "Doroški, Ana and Klaus, Anita and Kozarski, Maja and Cvetković, Stefana and Nikolić, Biljana and Jakovljević, Dragica and Tomasevic, Igor and Vunduk, Jovana and Lazić, Vesna and Djekic, Ilija",
year = "2020",
abstract = "Total quality index approach was used to reflect the quality of Pleurotus ostreatus
mushroom, cultivated on grape pomace (P) as food waste substrate and wheat straw
(S), in four different combined ratios: 100P; 80P20S; 50P50S; and 20P80S, with
100S, serving as control substrate. This approach involved two directions of mushroom
analysis. The whole mushroom fruiting bodies analysis introduced a single total
quality index (TQIM) based on 11 quality parameters. The mushroom water extracts
analysis was presented by another single total quality index (TQIE), with seven quality
parameters. This mathematical model revealed that the mushroom extracts have a
better overall quality score with higher pomace content in substrate mixture, compared
to the whole mushroom quality changes throughout the observed shelf life
period. Future research may pay attention to the phytotoxicity of grape pomace as a
food waste, as well as the influence of grape pomace on glucan content, as a healthbeneficial
compounds.",
publisher = "Wiley",
journal = "Journal of Food Processing and Preservation",
title = "The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus—Total quality index approach",
volume = "00:e15096",
doi = "10.1111/jfpp.15096"
}

Doroški, A., Klaus, A., Kozarski, M., Cvetković, S., Nikolić, B., Jakovljević, D., Tomasevic, I., Vunduk, J., Lazić, V.,& Djekic, I.. (2020). The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus—Total quality index approach. in Journal of Food Processing and Preservation
Wiley., 00:e15096.
https://doi.org/10.1111/jfpp.15096

Doroški A, Klaus A, Kozarski M, Cvetković S, Nikolić B, Jakovljević D, Tomasevic I, Vunduk J, Lazić V, Djekic I. The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus—Total quality index approach. in Journal of Food Processing and Preservation. 2020;00:e15096.
doi:10.1111/jfpp.15096 .

Doroški, Ana, Klaus, Anita, Kozarski, Maja, Cvetković, Stefana, Nikolić, Biljana, Jakovljević, Dragica, Tomasevic, Igor, Vunduk, Jovana, Lazić, Vesna, Djekic, Ilija, "The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus—Total quality index approach" in Journal of Food Processing and Preservation, 00:e15096 (2020),
https://doi.org/10.1111/jfpp.15096 . .

TY  - CONF
AU  - Klaus, A.
AU  - Kozarski, M.
AU  - Lazić, V.
AU  - Petrović, P.
AU  - Vunduk, J.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6530
AB  - From ancient times, in the tradition of many cultures forest mushrooms were used
for food, but they also played an important role in the treatment of numerous health
disorders. In modern life, people still face many health challenges, and one of the
most worrying is the growing resistance to antibiotics that are in widespread use.
Therefore, new sources of antimicrobial components are constantly being
researched, and mushrooms are one of the most important. In light of this
approach, the aim of current study was to determine the antilisterial potential of
water and alkali extracts obtained from mushrooms Ganoderma lucidum,
Ganoderma applanatum, Fomitopsis pinicola, Craterellus curnucopioides,
Meripilus giganteus and Boletus edulis. For all tested water extracts, a minimum
inhibitory concentration (MIC) towards Listeria monocytogenes ATCC 19111 was
found to be in the concentration range of 0.0—20 mg/mL, while this bacteria was
inhibited in the presence of 2.5—20 mg/mL of alkali extracts. A minimum
bactericidal concentration (MBC) of 20 mg/mL was determined for all observed
alkali extracts. Overall, the most pronounced antilisterial effect was confirmed in
G. applanatum and F. pinicola alkali extracts (MIC - 2.5 mg/mL, MBC - 20.0
mg/mL). The results of this study are very promising and indicate the potential use
of the tested extracts as antilisterial agents. Since mushrooms used for this research
are already known as culinary/medically important mushrooms, their extracts in the
production of functional food products would be welcome.
PB  - University in Banjaluka, Faculty of Technology
C3  - International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska
T1  - Antilisterial potential of selected forest mushrooms of Serbia
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6530
ER  - 

@conference{
author = "Klaus, A. and Kozarski, M. and Lazić, V. and Petrović, P. and Vunduk, J.",
year = "2020",
abstract = "From ancient times, in the tradition of many cultures forest mushrooms were used
for food, but they also played an important role in the treatment of numerous health
disorders. In modern life, people still face many health challenges, and one of the
most worrying is the growing resistance to antibiotics that are in widespread use.
Therefore, new sources of antimicrobial components are constantly being
researched, and mushrooms are one of the most important. In light of this
approach, the aim of current study was to determine the antilisterial potential of
water and alkali extracts obtained from mushrooms Ganoderma lucidum,
Ganoderma applanatum, Fomitopsis pinicola, Craterellus curnucopioides,
Meripilus giganteus and Boletus edulis. For all tested water extracts, a minimum
inhibitory concentration (MIC) towards Listeria monocytogenes ATCC 19111 was
found to be in the concentration range of 0.0—20 mg/mL, while this bacteria was
inhibited in the presence of 2.5—20 mg/mL of alkali extracts. A minimum
bactericidal concentration (MBC) of 20 mg/mL was determined for all observed
alkali extracts. Overall, the most pronounced antilisterial effect was confirmed in
G. applanatum and F. pinicola alkali extracts (MIC - 2.5 mg/mL, MBC - 20.0
mg/mL). The results of this study are very promising and indicate the potential use
of the tested extracts as antilisterial agents. Since mushrooms used for this research
are already known as culinary/medically important mushrooms, their extracts in the
production of functional food products would be welcome.",
publisher = "University in Banjaluka, Faculty of Technology",
journal = "International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska",
title = "Antilisterial potential of selected forest mushrooms of Serbia",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6530"
}

Klaus, A., Kozarski, M., Lazić, V., Petrović, P.,& Vunduk, J.. (2020). Antilisterial potential of selected forest mushrooms of Serbia. in International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska
University in Banjaluka, Faculty of Technology..
https://hdl.handle.net/21.15107/rcub_agrospace_6530

Klaus A, Kozarski M, Lazić V, Petrović P, Vunduk J. Antilisterial potential of selected forest mushrooms of Serbia. in International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska. 2020;.
https://hdl.handle.net/21.15107/rcub_agrospace_6530 .

Klaus, A., Kozarski, M., Lazić, V., Petrović, P., Vunduk, J., "Antilisterial potential of selected forest mushrooms of Serbia" in International scientific conference "XIII Conference of Chemists, Technologist and Environmentalists of Republic of Srpska (2020),
https://hdl.handle.net/21.15107/rcub_agrospace_6530 .

TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
AU  - Nikšić, Miomir
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5083
AB  - Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin's barrier function, and provide a skin-lightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction (HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50 = 1.65 +/- 0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes
EP  - 264
IS  - 2
SP  - 253
VL  - 71
DO  - 10.2298/ABS181217007K
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Wan-Mohtar, Wan Abd Al Qadr Imad and Nikšić, Miomir",
year = "2019",
abstract = "Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin's barrier function, and provide a skin-lightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction (HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50 = 1.65 +/- 0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes",
pages = "264-253",
number = "2",
volume = "71",
doi = "10.2298/ABS181217007K"
}

Kozarski, M., Klaus, A., Jakovljević, D., Todorović, N., Wan-Mohtar, W. A. A. Q. I.,& Nikšić, M.. (2019). Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(2), 253-264.
https://doi.org/10.2298/ABS181217007K

Kozarski M, Klaus A, Jakovljević D, Todorović N, Wan-Mohtar WAAQI, Nikšić M. Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences. 2019;71(2):253-264.
doi:10.2298/ABS181217007K .

Kozarski, Maja, Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, Nikšić, Miomir, "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes" in Archives of Biological Sciences, 71, no. 2 (2019):253-264,
https://doi.org/10.2298/ABS181217007K . .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Ivanović, Katarina
AU  - Jovanović, Aleksandra
AU  - Simović, Milica
AU  - Milutinović, Violeta
AU  - Kozarski, Maja
AU  - Petković, Milos
AU  - Cvetković, Anka
AU  - Klaus, Anita
AU  - Bugarski, Branko
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4975
AB  - Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products
EP  - 733
IS  - 4
SP  - 721
VL  - 71
DO  - 10.2298/ABS190725055P
ER  - 

@article{
author = "Petrović, Predrag and Ivanović, Katarina and Jovanović, Aleksandra and Simović, Milica and Milutinović, Violeta and Kozarski, Maja and Petković, Milos and Cvetković, Anka and Klaus, Anita and Bugarski, Branko",
year = "2019",
abstract = "Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products",
pages = "733-721",
number = "4",
volume = "71",
doi = "10.2298/ABS190725055P"
}

Petrović, P., Ivanović, K., Jovanović, A., Simović, M., Milutinović, V., Kozarski, M., Petković, M., Cvetković, A., Klaus, A.,& Bugarski, B.. (2019). The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(4), 721-733.
https://doi.org/10.2298/ABS190725055P

Petrović P, Ivanović K, Jovanović A, Simović M, Milutinović V, Kozarski M, Petković M, Cvetković A, Klaus A, Bugarski B. The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives of Biological Sciences. 2019;71(4):721-733.
doi:10.2298/ABS190725055P .

Petrović, Predrag, Ivanović, Katarina, Jovanović, Aleksandra, Simović, Milica, Milutinović, Violeta, Kozarski, Maja, Petković, Milos, Cvetković, Anka, Klaus, Anita, Bugarski, Branko, "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products" in Archives of Biological Sciences, 71, no. 4 (2019):721-733,
https://doi.org/10.2298/ABS190725055P . .

TY  - CONF
AU  - Doroški, Ana
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Vunduk, Jovana
AU  - Djekic, Ilija
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6507
AB  - Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders. Synthetic antioxidants can improve defence mechanisms, but their potential adverse toxic effects give priority to natural antioxidants. Pleurotus ostreatus (Jacq. ex Fr.) P. Kumer (1871) (oyster mushroom) is one of the most commonly cultivated mushrooms in the world due to its adaptibility to various substrates and having a specific mild taste of oyster in culinary processing. Because of the presence of various active ingredients it owns antidiabetic, antibacterial, anticholestrolic, antiarthritic, antioxidant, anticancer and antiviral activities.
The aim of this study was to monitor the influence of grape pomace supstrate on the antioxidative potential of oyster mushroom, as well as changes of antioxidant properties from the day of harvesting during the storage period of 7 and 14 days. The substrate used for cultivation of Pleurotus ostreatus was consisted of grape pomace and straw (20:80%) The fruiting bodies were air-dried at 55°C, powdered and then prepared as crude hot water extracts in mild conditions, at 75-85°C for 1.5 h. Concentration range of 0.625-40 mg/mL of each extract was analyzed. The radical absorbance ability of the extract was tested in vitro, using DPPH free radical and ABTS+ radical scavenging capability and expressed as EC50 (mg/mL) values.
Crude water extracts showed the following results: EC50 values of the DPPH scavenging ability on the 0th, 7th and 14th day were 30.25, 29.24 and 19.9 mg/mL, respectively. EC50 values of the ABTS+ scavenging activity of the extracts on the 0th, 7th and 14th day showed the difference, with following values: 8.1, 8.5 and 2.6 mg/mL, respectively. All investigated extracts showed good antioxidant abilities. Both methods confirmed decrease of the EC50 values and increasing of the antioxidative potential from harvesting day and during the storage period of 7 and 14 days.
PB  - Univerzitet u Beogradu / University of Belgrade
C3  - UNIFOOD CONFERENCE University of Belgrade 210th Anniversary
T1  - Antioxidative potential of the Oyster mushroom (Pleurotus ostreatus) cultivated on grape pomace and straw supstrate
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6507
ER  - 

@conference{
author = "Doroški, Ana and Kozarski, Maja and Klaus, Anita and Nikšić, Miomir and Vunduk, Jovana and Djekic, Ilija",
year = "2018",
abstract = "Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders. Synthetic antioxidants can improve defence mechanisms, but their potential adverse toxic effects give priority to natural antioxidants. Pleurotus ostreatus (Jacq. ex Fr.) P. Kumer (1871) (oyster mushroom) is one of the most commonly cultivated mushrooms in the world due to its adaptibility to various substrates and having a specific mild taste of oyster in culinary processing. Because of the presence of various active ingredients it owns antidiabetic, antibacterial, anticholestrolic, antiarthritic, antioxidant, anticancer and antiviral activities.
The aim of this study was to monitor the influence of grape pomace supstrate on the antioxidative potential of oyster mushroom, as well as changes of antioxidant properties from the day of harvesting during the storage period of 7 and 14 days. The substrate used for cultivation of Pleurotus ostreatus was consisted of grape pomace and straw (20:80%) The fruiting bodies were air-dried at 55°C, powdered and then prepared as crude hot water extracts in mild conditions, at 75-85°C for 1.5 h. Concentration range of 0.625-40 mg/mL of each extract was analyzed. The radical absorbance ability of the extract was tested in vitro, using DPPH free radical and ABTS+ radical scavenging capability and expressed as EC50 (mg/mL) values.
Crude water extracts showed the following results: EC50 values of the DPPH scavenging ability on the 0th, 7th and 14th day were 30.25, 29.24 and 19.9 mg/mL, respectively. EC50 values of the ABTS+ scavenging activity of the extracts on the 0th, 7th and 14th day showed the difference, with following values: 8.1, 8.5 and 2.6 mg/mL, respectively. All investigated extracts showed good antioxidant abilities. Both methods confirmed decrease of the EC50 values and increasing of the antioxidative potential from harvesting day and during the storage period of 7 and 14 days.",
publisher = "Univerzitet u Beogradu / University of Belgrade",
journal = "UNIFOOD CONFERENCE University of Belgrade 210th Anniversary",
title = "Antioxidative potential of the Oyster mushroom (Pleurotus ostreatus) cultivated on grape pomace and straw supstrate",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6507"
}

Doroški, A., Kozarski, M., Klaus, A., Nikšić, M., Vunduk, J.,& Djekic, I.. (2018). Antioxidative potential of the Oyster mushroom (Pleurotus ostreatus) cultivated on grape pomace and straw supstrate. in UNIFOOD CONFERENCE University of Belgrade 210th Anniversary
Univerzitet u Beogradu / University of Belgrade..
https://hdl.handle.net/21.15107/rcub_agrospace_6507

Doroški A, Kozarski M, Klaus A, Nikšić M, Vunduk J, Djekic I. Antioxidative potential of the Oyster mushroom (Pleurotus ostreatus) cultivated on grape pomace and straw supstrate. in UNIFOOD CONFERENCE University of Belgrade 210th Anniversary. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_6507 .

Doroški, Ana, Kozarski, Maja, Klaus, Anita, Nikšić, Miomir, Vunduk, Jovana, Djekic, Ilija, "Antioxidative potential of the Oyster mushroom (Pleurotus ostreatus) cultivated on grape pomace and straw supstrate" in UNIFOOD CONFERENCE University of Belgrade 210th Anniversary (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_6507 .

TY  - JOUR
AU  - Vunduk, Jovana
AU  - Djekić, Ilija
AU  - Petrović, Predrag
AU  - Tomašević, Igor
AU  - Kozarski, Maja
AU  - Despotović, Saša
AU  - Nikšić, Miomir
AU  - Klaus, Anita
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4774
AB  - Purpose The purpose of this paper is to examine compositional differences between brown and white varieties of Agaricus bisporus during shelf life and to determine if the growing demand for the brown variety is scientifically justified. Design/methodology/approach Field research enabled analyzing consumers' perceptions on intrinsic, extrinsic and quality characteristics of mushrooms. A total of 275 consumers participated in the survey. Obtained results were used for comparing white and brown varieties of A. bisporus over a period of 22 days. Mushrooms were packed in air and in modified atmosphere packaging (MAP) at 4 degrees C. Samples were analyzed for their nutritional, antioxidative and microbiological characteristics. Findings Two weeks from harvest, white variety showed higher amount of essential nutrients, while during the third week, brown mushrooms were more nutritionally valuable. Brown variety had better antioxidative ability for all 22 days of storage. Aerobic plate count (APC) was as expected for the mushrooms. MAPs significantly lowered APC in both varieties. The number of Enterobacteriaceae was equal for both varieties at the beginning, but later on they developed much faster in the case of brown variety. Field research combined with specific analyses clarified that there are no nutritive or microbiological reasons for the precedence of brown variety over white. Research limitations/implications Sensory aspect of the quality of mushrooms was not analyzed. Originality/value Market trend toward brown variety was scientifically challenged.
PB  - Emerald Group Publishing Ltd, Bingley
T2  - British Food Journal
T1  - Challenging the difference between white and brown Agaricus bisporus mushrooms: Science behind consumers choice
EP  - 1394
IS  - 6
SP  - 1381
VL  - 120
DO  - 10.1108/BFJ-10-2017-0550
ER  - 

@article{
author = "Vunduk, Jovana and Djekić, Ilija and Petrović, Predrag and Tomašević, Igor and Kozarski, Maja and Despotović, Saša and Nikšić, Miomir and Klaus, Anita",
year = "2018",
abstract = "Purpose The purpose of this paper is to examine compositional differences between brown and white varieties of Agaricus bisporus during shelf life and to determine if the growing demand for the brown variety is scientifically justified. Design/methodology/approach Field research enabled analyzing consumers' perceptions on intrinsic, extrinsic and quality characteristics of mushrooms. A total of 275 consumers participated in the survey. Obtained results were used for comparing white and brown varieties of A. bisporus over a period of 22 days. Mushrooms were packed in air and in modified atmosphere packaging (MAP) at 4 degrees C. Samples were analyzed for their nutritional, antioxidative and microbiological characteristics. Findings Two weeks from harvest, white variety showed higher amount of essential nutrients, while during the third week, brown mushrooms were more nutritionally valuable. Brown variety had better antioxidative ability for all 22 days of storage. Aerobic plate count (APC) was as expected for the mushrooms. MAPs significantly lowered APC in both varieties. The number of Enterobacteriaceae was equal for both varieties at the beginning, but later on they developed much faster in the case of brown variety. Field research combined with specific analyses clarified that there are no nutritive or microbiological reasons for the precedence of brown variety over white. Research limitations/implications Sensory aspect of the quality of mushrooms was not analyzed. Originality/value Market trend toward brown variety was scientifically challenged.",
publisher = "Emerald Group Publishing Ltd, Bingley",
journal = "British Food Journal",
title = "Challenging the difference between white and brown Agaricus bisporus mushrooms: Science behind consumers choice",
pages = "1394-1381",
number = "6",
volume = "120",
doi = "10.1108/BFJ-10-2017-0550"
}

Vunduk, J., Djekić, I., Petrović, P., Tomašević, I., Kozarski, M., Despotović, S., Nikšić, M.,& Klaus, A.. (2018). Challenging the difference between white and brown Agaricus bisporus mushrooms: Science behind consumers choice. in British Food Journal
Emerald Group Publishing Ltd, Bingley., 120(6), 1381-1394.
https://doi.org/10.1108/BFJ-10-2017-0550

Vunduk J, Djekić I, Petrović P, Tomašević I, Kozarski M, Despotović S, Nikšić M, Klaus A. Challenging the difference between white and brown Agaricus bisporus mushrooms: Science behind consumers choice. in British Food Journal. 2018;120(6):1381-1394.
doi:10.1108/BFJ-10-2017-0550 .

Vunduk, Jovana, Djekić, Ilija, Petrović, Predrag, Tomašević, Igor, Kozarski, Maja, Despotović, Saša, Nikšić, Miomir, Klaus, Anita, "Challenging the difference between white and brown Agaricus bisporus mushrooms: Science behind consumers choice" in British Food Journal, 120, no. 6 (2018):1381-1394,
https://doi.org/10.1108/BFJ-10-2017-0550 . .

TY  - JOUR
AU  - Đekić, Ilija
AU  - Vunduk, Jovana
AU  - Tomašević, Igor
AU  - Kozarski, Maja
AU  - Petrović, Predrag
AU  - Nikšić, Miomir
AU  - Klaus, Anita
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6054
AB  - BACKGROUND: The aim of this study was to develop a total quality index and examine the effects of modified atmosphere
packaging (MAP) on the quality of Agaricus bisporus mushrooms stored for 22 days at 4 ∘C. Mushrooms were packaged under
three MAPs: high nitrogen packaging (HNP), low carbon dioxide packaging (LCP) and low oxygen packaging (LOP). Passive MAP
with air inside initially was used as the atmosphere treatment (AIR).
RESULTS: This research revealed two phases in quality deterioration of A. bisporus mushrooms. During the first week, most of
the quality parameters were not statistically different. Thereafter, odor intensities were stronger for all four types of packaging.
Color difference and browning index values showed significantly lower color changes for AIR and LOP compared with HNP and
LCP mushrooms.
CONCLUSION: The best total quality index was calculated for LOP, followed by LCP and AIR. The findings of this study are
useful with respect to examining two-component MAPs, separating the limiting factors (O2 and CO2) and evaluating quality
deterioration effects and the total quality index of A. bisporus mushrooms.
© 2016 Society of Chemical Industry
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere
EP  - 3021
IS  - 9
SP  - 3013
VL  - 97
DO  - 10.1002/jsfa.8142
ER  - 

@article{
author = "Đekić, Ilija and Vunduk, Jovana and Tomašević, Igor and Kozarski, Maja and Petrović, Predrag and Nikšić, Miomir and Klaus, Anita",
year = "2017",
abstract = "BACKGROUND: The aim of this study was to develop a total quality index and examine the effects of modified atmosphere
packaging (MAP) on the quality of Agaricus bisporus mushrooms stored for 22 days at 4 ∘C. Mushrooms were packaged under
three MAPs: high nitrogen packaging (HNP), low carbon dioxide packaging (LCP) and low oxygen packaging (LOP). Passive MAP
with air inside initially was used as the atmosphere treatment (AIR).
RESULTS: This research revealed two phases in quality deterioration of A. bisporus mushrooms. During the first week, most of
the quality parameters were not statistically different. Thereafter, odor intensities were stronger for all four types of packaging.
Color difference and browning index values showed significantly lower color changes for AIR and LOP compared with HNP and
LCP mushrooms.
CONCLUSION: The best total quality index was calculated for LOP, followed by LCP and AIR. The findings of this study are
useful with respect to examining two-component MAPs, separating the limiting factors (O2 and CO2) and evaluating quality
deterioration effects and the total quality index of A. bisporus mushrooms.
© 2016 Society of Chemical Industry",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere",
pages = "3021-3013",
number = "9",
volume = "97",
doi = "10.1002/jsfa.8142"
}

Đekić, I., Vunduk, J., Tomašević, I., Kozarski, M., Petrović, P., Nikšić, M.,& Klaus, A.. (2017). Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere. in Journal of the Science of Food and Agriculture
Wiley., 97(9), 3013-3021.
https://doi.org/10.1002/jsfa.8142

Đekić I, Vunduk J, Tomašević I, Kozarski M, Petrović P, Nikšić M, Klaus A. Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere. in Journal of the Science of Food and Agriculture. 2017;97(9):3013-3021.
doi:10.1002/jsfa.8142 .

Đekić, Ilija, Vunduk, Jovana, Tomašević, Igor, Kozarski, Maja, Petrović, Predrag, Nikšić, Miomir, Klaus, Anita, "Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere" in Journal of the Science of Food and Agriculture, 97, no. 9 (2017):3013-3021,
https://doi.org/10.1002/jsfa.8142 . .