TY  - GEN
AU  - Pantić, Milena
AU  - Miletić, Dunja
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6619
AB  - Selenium (Se) is an essential micronutrient which bioavailability and toxicity depends on its form and
concentration that is taken into the body. Se uptake from organic sources goes up to 90-95%, while
from inorganic it’s up to 10%. In addition, toxicity of organic Se compounds is low. In the last decades,
the interest in functional foods and natural dietary supplements consummation has been growing.
Since the mushroom market also constantly grows, bioactive compounds, isolated from higher fungi
grown on fortified substrates, are their promising source. During last 15 years of research, the biological
activity of Se-enriched macromycetes has been proven through in vitro tests by our group. The
research was conducted on selenium enriched mushroom species (Pleurotus spp, Lentinus edodes,
Coriolus versicolor and Agaricus bisporus) obtained using solid state and submerged cultivation techniques,
and different sources of selenium for the growing substrate supplementation.
Chemical composition analysis revealed that, mostly, Se-enriched extracts and polysaccharides obtained
from mycelia and fruiting bodies had higher content of glucans, total proteins and phenolic
compounds compared to non-enriched samples. Also, a significant percentage of total accumulated
Se was incorporated in the form of L-selenomethionine. Mushrooms enriched with Se from an organic
source (selenourea, Se-yeast) showed significantly different Se-accumulation efficiency, biomass yield,
content of the L-selenomethionine, Se content and different biological activity, compared to the mushroom
samples enriched with inorganic salts (sodium selenate and sodium selenite).
The ability of some mushroom species to grow and accumulate selenium also varied on used cultivation
technique and growth stage. In vitro test of the fresh fruiting bodies indicated the positive influence
of Se from Se-modified zeolites on the activity of enzymes of primary antioxidant protection - superoxide
dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT).Antioxidant activity of selenium enriched extracts depended on the Se concentration, form of the Se
used for the supplementation, mushroom strain and type of the mushroom extract. An increase in the
activity was noted with increasing concentration of Se in all tested samples.
Furthermore, antibacterial activity against food-borne pathogens and non-toxicity to moderate toxicity
of extracts, with high content of Se, against normal MRC-5 cell line was proven. According to results,
Se-enriched mushrooms in the form of fruiting bodies, mycelia, extracts or polysaccharide extracts
could be a promising non-toxic dietary supplements or functional food/additives with prolonged shelf
life and improved bioactivity.
T2  - 11th International Medicinal Mushroom Conference (IMMC11)
T1  - Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry
EP  - 140
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6619
ER  - 

@misc{
author = "Pantić, Milena and Miletić, Dunja and Matijašević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2022",
abstract = "Selenium (Se) is an essential micronutrient which bioavailability and toxicity depends on its form and
concentration that is taken into the body. Se uptake from organic sources goes up to 90-95%, while
from inorganic it’s up to 10%. In addition, toxicity of organic Se compounds is low. In the last decades,
the interest in functional foods and natural dietary supplements consummation has been growing.
Since the mushroom market also constantly grows, bioactive compounds, isolated from higher fungi
grown on fortified substrates, are their promising source. During last 15 years of research, the biological
activity of Se-enriched macromycetes has been proven through in vitro tests by our group. The
research was conducted on selenium enriched mushroom species (Pleurotus spp, Lentinus edodes,
Coriolus versicolor and Agaricus bisporus) obtained using solid state and submerged cultivation techniques,
and different sources of selenium for the growing substrate supplementation.
Chemical composition analysis revealed that, mostly, Se-enriched extracts and polysaccharides obtained
from mycelia and fruiting bodies had higher content of glucans, total proteins and phenolic
compounds compared to non-enriched samples. Also, a significant percentage of total accumulated
Se was incorporated in the form of L-selenomethionine. Mushrooms enriched with Se from an organic
source (selenourea, Se-yeast) showed significantly different Se-accumulation efficiency, biomass yield,
content of the L-selenomethionine, Se content and different biological activity, compared to the mushroom
samples enriched with inorganic salts (sodium selenate and sodium selenite).
The ability of some mushroom species to grow and accumulate selenium also varied on used cultivation
technique and growth stage. In vitro test of the fresh fruiting bodies indicated the positive influence
of Se from Se-modified zeolites on the activity of enzymes of primary antioxidant protection - superoxide
dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT).Antioxidant activity of selenium enriched extracts depended on the Se concentration, form of the Se
used for the supplementation, mushroom strain and type of the mushroom extract. An increase in the
activity was noted with increasing concentration of Se in all tested samples.
Furthermore, antibacterial activity against food-borne pathogens and non-toxicity to moderate toxicity
of extracts, with high content of Se, against normal MRC-5 cell line was proven. According to results,
Se-enriched mushrooms in the form of fruiting bodies, mycelia, extracts or polysaccharide extracts
could be a promising non-toxic dietary supplements or functional food/additives with prolonged shelf
life and improved bioactivity.",
journal = "11th International Medicinal Mushroom Conference (IMMC11)",
title = "Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry",
pages = "140-139",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6619"
}

Pantić, M., Miletić, D., Matijašević, D., Sknepnek, A.,& Nikšić, M.. (2022). Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry. in 11th International Medicinal Mushroom Conference (IMMC11), 139-140.
https://hdl.handle.net/21.15107/rcub_agrospace_6619

Pantić M, Miletić D, Matijašević D, Sknepnek A, Nikšić M. Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry. in 11th International Medicinal Mushroom Conference (IMMC11). 2022;:139-140.
https://hdl.handle.net/21.15107/rcub_agrospace_6619 .

Pantić, Milena, Miletić, Dunja, Matijašević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Potential application of selenium-enriched mushrooms in the food and pharmaceutical industry" in 11th International Medicinal Mushroom Conference (IMMC11) (2022):139-140,
https://hdl.handle.net/21.15107/rcub_agrospace_6619 .

TY  - JOUR
AU  - Matijašević, Danka
AU  - Pantić, Milena
AU  - Stanisavljević, Nemanja
AU  - Jevtić, Sanja
AU  - Rajić, Nevenka
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6075
AB  - Research background. In the recent years, considerable attention has been given to selenium status since its deficiency is linked with various disorders and affects at least 13 % of world population. Additionally, mushrooms are known to possess pronounced capacity for absorption of various micronutrients, including Se, from soil/substrate. Here, we investigate the possibility of using Se-rich zeolitic tuff as a supplement for production of selenized mushroom. Furthermore, the impact of the enrichment on the activity of anti-oxidant enzymes and biological potential of Coriolus versicolor medicinal mushroom is studied. Experimental approach. Se(IV)-and Se(VI)-modified natural zeolitic tuff from the Ser-bian deposit Zlatokop was used as supplement for mushroom cultivation. To examine the effectiveness of selenium enrichment, we determined total selenium with inductively coupled plasma mass spectrometry (ICP-MS), together with the activity of antioxidant enzymes in fresh fruiting bodies and biological potential of methanolic extracts. Antioxidant activity was evaluated using the appropriate tests for: inhibition of lipid peroxidation, DPPH free radical scavenging assay, Fe(III)-reducing antioxidant power assay and ability of chelating Fe2+ ions. The antibacterial activity against foodborne pathogens was measured by broth microdilution assay. Additionally, chemical composition of the prepared extracts was studied using UV-Vis and Fourier transform infrared (FTIR) spectroscopy. Results and conclusions. Content of selenium detected in biofortified C. versicolor was even 470 times higher than in control on dry mass basis ((140.7±3.8) vs (0.3±0.1) µg/g), proving that Se-rich zeolitic tuff is an excellent supplement for mushroom production. Furthermore, the results of monitoring the activity of antioxidant enzymes revealed that most of the Se-enriched mushrooms exhibited higher superoxide dismutase (SOD) and catalase (CAT) and lower glutathione peroxidase (GSH-Px) activities than control. Due to higher amounts of enzymes, which can quickly catalyze the reduction of superoxide radicals, the quality of selenium-enriched mushrooms is preserved for a longer period of time. Investigation of biological potential indicated that Se-enriched mushroom methanolic extracts, generally, expressed enhanced antioxidant properties. Additionally, extracts showed antibacterial activity against all tested pathogenic microorganisms. Novelty and scientific contribution. Cultivation of mushrooms on Se-enriched zeolitic tuff is a new technological approach for obtaining Se-fortified food/supplements with enhanced antioxidant and antibacterial activities.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement
EP  - 79
IS  - 1
SP  - 67
VL  - 60
DO  - 10.17113/ftb.60.01.22.7172
ER  - 

@article{
author = "Matijašević, Danka and Pantić, Milena and Stanisavljević, Nemanja and Jevtić, Sanja and Rajić, Nevenka and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2022",
abstract = "Research background. In the recent years, considerable attention has been given to selenium status since its deficiency is linked with various disorders and affects at least 13 % of world population. Additionally, mushrooms are known to possess pronounced capacity for absorption of various micronutrients, including Se, from soil/substrate. Here, we investigate the possibility of using Se-rich zeolitic tuff as a supplement for production of selenized mushroom. Furthermore, the impact of the enrichment on the activity of anti-oxidant enzymes and biological potential of Coriolus versicolor medicinal mushroom is studied. Experimental approach. Se(IV)-and Se(VI)-modified natural zeolitic tuff from the Ser-bian deposit Zlatokop was used as supplement for mushroom cultivation. To examine the effectiveness of selenium enrichment, we determined total selenium with inductively coupled plasma mass spectrometry (ICP-MS), together with the activity of antioxidant enzymes in fresh fruiting bodies and biological potential of methanolic extracts. Antioxidant activity was evaluated using the appropriate tests for: inhibition of lipid peroxidation, DPPH free radical scavenging assay, Fe(III)-reducing antioxidant power assay and ability of chelating Fe2+ ions. The antibacterial activity against foodborne pathogens was measured by broth microdilution assay. Additionally, chemical composition of the prepared extracts was studied using UV-Vis and Fourier transform infrared (FTIR) spectroscopy. Results and conclusions. Content of selenium detected in biofortified C. versicolor was even 470 times higher than in control on dry mass basis ((140.7±3.8) vs (0.3±0.1) µg/g), proving that Se-rich zeolitic tuff is an excellent supplement for mushroom production. Furthermore, the results of monitoring the activity of antioxidant enzymes revealed that most of the Se-enriched mushrooms exhibited higher superoxide dismutase (SOD) and catalase (CAT) and lower glutathione peroxidase (GSH-Px) activities than control. Due to higher amounts of enzymes, which can quickly catalyze the reduction of superoxide radicals, the quality of selenium-enriched mushrooms is preserved for a longer period of time. Investigation of biological potential indicated that Se-enriched mushroom methanolic extracts, generally, expressed enhanced antioxidant properties. Additionally, extracts showed antibacterial activity against all tested pathogenic microorganisms. Novelty and scientific contribution. Cultivation of mushrooms on Se-enriched zeolitic tuff is a new technological approach for obtaining Se-fortified food/supplements with enhanced antioxidant and antibacterial activities.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement",
pages = "79-67",
number = "1",
volume = "60",
doi = "10.17113/ftb.60.01.22.7172"
}

Matijašević, D., Pantić, M., Stanisavljević, N., Jevtić, S., Rajić, N., Lević, S., Nedović, V.,& Nikšić, M.. (2022). Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement. in Food Technology and Biotechnology
University of Zagreb., 60(1), 67-79.
https://doi.org/10.17113/ftb.60.01.22.7172

Matijašević D, Pantić M, Stanisavljević N, Jevtić S, Rajić N, Lević S, Nedović V, Nikšić M. Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement. in Food Technology and Biotechnology. 2022;60(1):67-79.
doi:10.17113/ftb.60.01.22.7172 .

Matijašević, Danka, Pantić, Milena, Stanisavljević, Nemanja, Jevtić, Sanja, Rajić, Nevenka, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement" in Food Technology and Biotechnology, 60, no. 1 (2022):67-79,
https://doi.org/10.17113/ftb.60.01.22.7172 . .

TY  - CONF
AU  - Pantic, Milena
AU  - Miletić, Dunja
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6645
AB  - Edible and medicinal mushrooms have been used worldwide due to their nutritional 
value and known pharmaceutical properties, which are related to the physiologically 
beneficial substances in the form of primary and secondary metabolites. Their chemical 
composition and biological activity depend on the medium on which they grow and applied 
supplementation. Numerous studies have shown that mushrooms can absorb selenium (Se) 
during growth on the fortified solid substrate or liquid medium, in a concentration that can 
meet the daily needs of humans. The capacity of some mushroom to absorb Se depends on 
multiple factors, including mushroom strain, composition of the medium, ecological pa rameters as well as on the form and concentration in which this mineral is added to the me dium. It was previously reported that strains Pleurotus sp, Agaricus bisporus, Ganoderma lu cidum, and Cordyceps militaris absorbed high concentrations of Se when sodium selenite or 
selenate were applied. However, higher toxicity of inorganic salts that suppressed mycelium 
growth of Hericium erinaceum, Lentinus edodes, Coriolus versicolor, and Pleurotus spp. com paring to modified zeolites with Se and organic selenitetriglycerides from Selol, Se-yeast, 
and selenourea was proven. Chemical characterization of polysaccharide and methanol ex tracts isolated from fruiting bodies or mycelia culture showed that most of the Se-enriched 
mushrooms had a higher content of total carbohydrates (especially β-glucans), proteins, fla vonoids, and phenols comparing to the non-enriched samples. As a consequence, selenized 
mushrooms showed improved antioxidant and antimicrobial properties. A significant per centage of Se was incorporated not only in the form of L-selenomethionine, but also other 
organic compounds, such as biologically active polysaccharides. It is believed that the syn ergism of Se and bioactive polysaccharides present in fungi could enhance their antitumor 
and immunostimulating functions, which lead to numerous health benefits. Obtained re sults classify Se-polysaccharides as promising dietary supplements and Se-enriched mush rooms as delicious functional food or food additives, due to the slow release of selenium 
and lower toxicity compared to the inorganic compounds. Selenium fortified mushrooms 
and their extracts used as food additives are expected to prolong the shelf life of the food 
products since some of them are proved to exhibit higher superoxide dismutase and catalase 
activity retaining their quality, aroma, and color for a long time of storage.
PB  - SERBIAN NUTRITION SOCIETY
C3  - 14th international Congress of Nutrition (CONU2021)
T1  - Selenium enriched mushrooms as food additives and dietary supplements
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6645
ER  - 

@conference{
author = "Pantic, Milena and Miletić, Dunja and Matijašević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2021",
abstract = "Edible and medicinal mushrooms have been used worldwide due to their nutritional 
value and known pharmaceutical properties, which are related to the physiologically 
beneficial substances in the form of primary and secondary metabolites. Their chemical 
composition and biological activity depend on the medium on which they grow and applied 
supplementation. Numerous studies have shown that mushrooms can absorb selenium (Se) 
during growth on the fortified solid substrate or liquid medium, in a concentration that can 
meet the daily needs of humans. The capacity of some mushroom to absorb Se depends on 
multiple factors, including mushroom strain, composition of the medium, ecological pa rameters as well as on the form and concentration in which this mineral is added to the me dium. It was previously reported that strains Pleurotus sp, Agaricus bisporus, Ganoderma lu cidum, and Cordyceps militaris absorbed high concentrations of Se when sodium selenite or 
selenate were applied. However, higher toxicity of inorganic salts that suppressed mycelium 
growth of Hericium erinaceum, Lentinus edodes, Coriolus versicolor, and Pleurotus spp. com paring to modified zeolites with Se and organic selenitetriglycerides from Selol, Se-yeast, 
and selenourea was proven. Chemical characterization of polysaccharide and methanol ex tracts isolated from fruiting bodies or mycelia culture showed that most of the Se-enriched 
mushrooms had a higher content of total carbohydrates (especially β-glucans), proteins, fla vonoids, and phenols comparing to the non-enriched samples. As a consequence, selenized 
mushrooms showed improved antioxidant and antimicrobial properties. A significant per centage of Se was incorporated not only in the form of L-selenomethionine, but also other 
organic compounds, such as biologically active polysaccharides. It is believed that the syn ergism of Se and bioactive polysaccharides present in fungi could enhance their antitumor 
and immunostimulating functions, which lead to numerous health benefits. Obtained re sults classify Se-polysaccharides as promising dietary supplements and Se-enriched mush rooms as delicious functional food or food additives, due to the slow release of selenium 
and lower toxicity compared to the inorganic compounds. Selenium fortified mushrooms 
and their extracts used as food additives are expected to prolong the shelf life of the food 
products since some of them are proved to exhibit higher superoxide dismutase and catalase 
activity retaining their quality, aroma, and color for a long time of storage.",
publisher = "SERBIAN NUTRITION SOCIETY",
journal = "14th international Congress of Nutrition (CONU2021)",
title = "Selenium enriched mushrooms as food additives and dietary supplements",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6645"
}

Pantic, M., Miletić, D., Matijašević, D., Sknepnek, A.,& Nikšić, M.. (2021). Selenium enriched mushrooms as food additives and dietary supplements. in 14th international Congress of Nutrition (CONU2021)
SERBIAN NUTRITION SOCIETY., 71.
https://hdl.handle.net/21.15107/rcub_agrospace_6645

Pantic M, Miletić D, Matijašević D, Sknepnek A, Nikšić M. Selenium enriched mushrooms as food additives and dietary supplements. in 14th international Congress of Nutrition (CONU2021). 2021;:71.
https://hdl.handle.net/21.15107/rcub_agrospace_6645 .

Pantic, Milena, Miletić, Dunja, Matijašević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Selenium enriched mushrooms as food additives and dietary supplements" in 14th international Congress of Nutrition (CONU2021) (2021):71,
https://hdl.handle.net/21.15107/rcub_agrospace_6645 .

TY  - CONF
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Lević, Steva
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Pavlović, Vladimir
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6833
AB  - Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other
activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting
body and mycelia are recognized as potential functional food and dietary supplement. On the
market, there are already many products based on the fungi extracts. For certain types of
mushrooms, submerged production of bioactive compounds stands out as the best method from
the standpoint of industrial applications. Mushrooms are becoming an attractive functional food
and overview about type of food suitable for enrichment with this valuable compounds were
given.
The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate
bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth
(exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential.
During fermentation residual glucose was quantified by HPLC. Mycelia products were
characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of
isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative
bacteria strains using broth microdilution method. Results showed that after 48h glucose
concentration started to decrease and after day seven slowed down, when process was
terminated saving energy and time. Applied gentle stirring during cultivation provided constant
oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy
pellets with long, branched and anastomosed hyphae of high density were formed. In prepared
mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex
chemical composition of methanol extracts comparing to EPS was noted. That might be the
explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol
extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample
showed significant activity against both Gram-positive and Gram-negative strains. Based on
obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were
more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising
process for bioactive compounds production.
C3  - 2nd B-fost 2018 Congress 15-17 October Yerevan
T1  - Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6833
ER  - 

@conference{
author = "Miletić, Dunja and Pantić, Milena and Lević, Steva and Matijašević, Danka and Sknepnek, Aleksandra and Pavlović, Vladimir and Nedović, Viktor and Nikšić, Miomir",
year = "2018",
abstract = "Researches have reported antimicrobial, antioxidative, antitumor, immunomodulatory and other
activities of more than 2000 mushroom species. Thanks to the promising results, both, fruiting
body and mycelia are recognized as potential functional food and dietary supplement. On the
market, there are already many products based on the fungi extracts. For certain types of
mushrooms, submerged production of bioactive compounds stands out as the best method from
the standpoint of industrial applications. Mushrooms are becoming an attractive functional food
and overview about type of food suitable for enrichment with this valuable compounds were
given.
The aim of the research was also to grow Coriolus versicolor mushroom in liquid culture, to isolate
bioactive compounds from obtained biomass (by methanol extraction), and fermentation broth
(exopolysaccharides EPS, by ethanol precipitation), and to determine their antimicrobial potential.
During fermentation residual glucose was quantified by HPLC. Mycelia products were
characterized by FTIR spectroscopy and scanning electron microscopy. Antibacterial activity of
isolated bioactive compounds was tested against eight Gram-positive and ten Gram-negative
bacteria strains using broth microdilution method. Results showed that after 48h glucose
concentration started to decrease and after day seven slowed down, when process was
terminated saving energy and time. Applied gentle stirring during cultivation provided constant
oxygen and nutrient supply and pellet fragmentation was avoided. As a result, preferred fluffy
pellets with long, branched and anastomosed hyphae of high density were formed. In prepared
mycelia samples, bands characteristic for polysaccharides were revealed. Also, more complex
chemical composition of methanol extracts comparing to EPS was noted. That might be the
explanation for the differences in antimicrobial activity. Microbicidal activity of mycelia methanol
extract (20 to 40 mg/ml) was recorded against seven tested Gram-negative bacteria. This sample
showed significant activity against both Gram-positive and Gram-negative strains. Based on
obtained inhibitory and bicidal concentrations, it can be concluded that Gram-positive strains were
more susceptible to EPS. Results makes submerged cultivation of C. versicolor as a prommising
process for bioactive compounds production.",
journal = "2nd B-fost 2018 Congress 15-17 October Yerevan",
title = "Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6833"
}

Miletić, D., Pantić, M., Lević, S., Matijašević, D., Sknepnek, A., Pavlović, V., Nedović, V.,& Nikšić, M.. (2018). Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production. in 2nd B-fost 2018 Congress 15-17 October Yerevan.
https://hdl.handle.net/21.15107/rcub_agrospace_6833

Miletić D, Pantić M, Lević S, Matijašević D, Sknepnek A, Pavlović V, Nedović V, Nikšić M. Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production. in 2nd B-fost 2018 Congress 15-17 October Yerevan. 2018;.
https://hdl.handle.net/21.15107/rcub_agrospace_6833 .

Miletić, Dunja, Pantić, Milena, Lević, Steva, Matijašević, Danka, Sknepnek, Aleksandra, Pavlović, Vladimir, Nedović, Viktor, Nikšić, Miomir, "Mushrooms as food and supplements  - batch culture fermentation of Coriolus versicolor as a promising process for antibacterial compounds production" in 2nd B-fost 2018 Congress 15-17 October Yerevan (2018),
https://hdl.handle.net/21.15107/rcub_agrospace_6833 .

TY  - CONF
AU  - Sknepnek, Aleksandra
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6845
AB  - Kombuha je fermentisani napitak koji potiče iz Mandčurije. U današnje vreme je postao veoma popularan i koristi se širom sveta. Vreli vodeni ekstrakti gljive Ganoderma lucidum je prvi put korišćen za pripremu kombuha produkta sa poboljšanim zdravstvenim efektom. Cilj ovog rada bio je određivanje hemijskog sastava novog napitka korišćenjem infracrvene spektroskopije sa Furijeovom transformacijom (FTIR) kao i ispitivanje njegove antioksidativne antibakterijske aktivnosti (ABA). Rezultati pokazuju da ovo piće može imati antibakterijsku aktivnost i zaštitnu ulogu kod oksidativnih oštećenja. Ovaj napitak se preporučuje kao funkcionalna hrana sa unapređenim zdravstvenim efektima i kao potencijalni atraktivni proizvod.
PB  - Zrenjanin
C3  - Saopštenje sa međunarodnog skupa štampano u izvodu
T1  - Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum
EP  - 61
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6845
ER  - 

@conference{
author = "Sknepnek, Aleksandra and Pantić, Milena and Matijašević, Danka and Miletić, Dunja and Lević, Steva and Nikšić, Miomir",
year = "2018",
abstract = "Kombuha je fermentisani napitak koji potiče iz Mandčurije. U današnje vreme je postao veoma popularan i koristi se širom sveta. Vreli vodeni ekstrakti gljive Ganoderma lucidum je prvi put korišćen za pripremu kombuha produkta sa poboljšanim zdravstvenim efektom. Cilj ovog rada bio je određivanje hemijskog sastava novog napitka korišćenjem infracrvene spektroskopije sa Furijeovom transformacijom (FTIR) kao i ispitivanje njegove antioksidativne antibakterijske aktivnosti (ABA). Rezultati pokazuju da ovo piće može imati antibakterijsku aktivnost i zaštitnu ulogu kod oksidativnih oštećenja. Ovaj napitak se preporučuje kao funkcionalna hrana sa unapređenim zdravstvenim efektima i kao potencijalni atraktivni proizvod.",
publisher = "Zrenjanin",
journal = "Saopštenje sa međunarodnog skupa štampano u izvodu",
title = "Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum",
pages = "61-60",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6845"
}

Sknepnek, A., Pantić, M., Matijašević, D., Miletić, D., Lević, S.,& Nikšić, M.. (2018). Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum. in Saopštenje sa međunarodnog skupa štampano u izvodu
Zrenjanin., 60-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6845

Sknepnek A, Pantić M, Matijašević D, Miletić D, Lević S, Nikšić M. Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum. in Saopštenje sa međunarodnog skupa štampano u izvodu. 2018;:60-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6845 .

Sknepnek, Aleksandra, Pantić, Milena, Matijašević, Danka, Miletić, Dunja, Lević, Steva, Nikšić, Miomir, "Zdravstveni efekti novog kombuha napitka od gljive Ganoderma lucidum" in Saopštenje sa međunarodnog skupa štampano u izvodu (2018):60-61,
https://hdl.handle.net/21.15107/rcub_agrospace_6845 .

TY  - CONF
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Miletić, Dunja
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6847
AB  - : Industrijski gajene gljive imaju veoma izraženu nutritivnu vrednost i predstavljaju bogat
izvor esencijalnih aminokiselina, vitamina, mineralnih materija, ugljenih hidrata, proteina kao i
važnih sekundarnih metabolita. Tokom višegodišnjih istraživanja dokazano je da gljiva može
uspešno usvojiti selen iz čvrstog supstrata ili tečne podloge u koncentraciji koja može da zadovolji
dnevne potrebe čoveka. Kapacitet usvojivosti selena zavisi od vrste gljive, hranljive podloge,
ekoloških parametara kao i od oblika i koncentraciјe u kom јe selen dodat u podlogu. Kako gljive
sadrže brojne bioaktivne komponente u vidu primarnih i sekundarnih metabolita, dodatno
selenom obogaćene pečurke moguće јe koristiti u svežem i sušenom stanju ili kao potencijalni
dijetetski suplement. Ispitana je biološka aktivnost selenom obogaćenih gljiva Coriolus versicolor
i Pleurotus ostreatus. Kao izvori selena upotrebljena su organska jedinjenja u obliku selenskog
kvasca i selenouree, kao i Se(IV)- i Se(VI)-modifikovani zeoliti.
PB  - Udruženje mikrobiologa Srbije, Nemanjina 6, Beograd
C3  - Predavanje po pozivu sa međunarodnog skupa štampano u celini
T1  - Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom
EP  - 152
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6847
ER  - 

@conference{
author = "Pantić, Milena and Matijašević, Danka and Miletić, Dunja and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2018",
abstract = ": Industrijski gajene gljive imaju veoma izraženu nutritivnu vrednost i predstavljaju bogat
izvor esencijalnih aminokiselina, vitamina, mineralnih materija, ugljenih hidrata, proteina kao i
važnih sekundarnih metabolita. Tokom višegodišnjih istraživanja dokazano je da gljiva može
uspešno usvojiti selen iz čvrstog supstrata ili tečne podloge u koncentraciji koja može da zadovolji
dnevne potrebe čoveka. Kapacitet usvojivosti selena zavisi od vrste gljive, hranljive podloge,
ekoloških parametara kao i od oblika i koncentraciјe u kom јe selen dodat u podlogu. Kako gljive
sadrže brojne bioaktivne komponente u vidu primarnih i sekundarnih metabolita, dodatno
selenom obogaćene pečurke moguće јe koristiti u svežem i sušenom stanju ili kao potencijalni
dijetetski suplement. Ispitana je biološka aktivnost selenom obogaćenih gljiva Coriolus versicolor
i Pleurotus ostreatus. Kao izvori selena upotrebljena su organska jedinjenja u obliku selenskog
kvasca i selenouree, kao i Se(IV)- i Se(VI)-modifikovani zeoliti.",
publisher = "Udruženje mikrobiologa Srbije, Nemanjina 6, Beograd",
journal = "Predavanje po pozivu sa međunarodnog skupa štampano u celini",
title = "Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom",
pages = "152-150",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6847"
}

Pantić, M., Matijašević, D., Miletić, D., Sknepnek, A.,& Nikšić, M.. (2018). Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom. in Predavanje po pozivu sa međunarodnog skupa štampano u celini
Udruženje mikrobiologa Srbije, Nemanjina 6, Beograd., 150-152.
https://hdl.handle.net/21.15107/rcub_agrospace_6847

Pantić M, Matijašević D, Miletić D, Sknepnek A, Nikšić M. Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom. in Predavanje po pozivu sa međunarodnog skupa štampano u celini. 2018;:150-152.
https://hdl.handle.net/21.15107/rcub_agrospace_6847 .

Pantić, Milena, Matijašević, Danka, Miletić, Dunja, Sknepnek, Aleksandra, Nikšić, Miomir, "Biološki potencijal odabranih vrsta makromiceta obogaćenih selenom" in Predavanje po pozivu sa međunarodnog skupa štampano u celini (2018):150-152,
https://hdl.handle.net/21.15107/rcub_agrospace_6847 .

TY  - JOUR
AU  - Karličić, Vera
AU  - Živanović, Iva
AU  - Matijašević, Danka
AU  - Raičević, Vera
AU  - Nikšić, Miomir
AU  - Rac, Vladislav
AU  - Simić, Aleksandar
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4607
AB  - Zeolites can mitigate ammonium losses to the environment by taking up the ions from sources such as farmyard manure and ammonium-bearing fertilizers. This ability can improve nitrogen fertilizing practice given that ammonium ions loaded onto zeolite are available for plant uptake. The main goal of this study was to assess the influence of zeolite (clinoptilolite) and ammonia-loaded clinoptilolite on growth and yield of red clover (Trifolium pratense L.), as well as their influence on the microbial dynamics in soil. Plants sown in pots were cut four times, and dry matter yield (DM) of each harvest was weighed. The number of culturable bacteria, moulds, ammonifiers, Azotobacter sp. and actinomycetes was determined at the beginning of the experiment, and after each harvest. Two control treatments (soil without fertilizer and soil supplemented with mineral fertilizer - CAN) were included in the study. The application of ammonia-loaded clinoptilolite positively and significantly affected the microbial activity and provided higher yields (from 4.9 g/pot in ammonia-loaded clinoptilolite treatment to 4.3 g/pot in soil with zeolite treatment). Our results suggest that the addition of clinoptilolite to organic fertilizers (manure, composts) could be recommended. The increase of microbial communities’ abundances and their activity represented the key benefit from clinoptilolite application.
AB  - Zeoliti mogu ublažiti otpuštanje amonijaka u prirodu preko uzimanja jona iz izvora kao što su stajnjak i đubriva bogata amonijakom. Ova sposobnost može unaprediti đubrenje azotom preko zeolitom usvojenih amonijum jona koji bi tako postali dostupni za usvajanje biljkama. Cilj ovih ispitivanja je bio procena uticaja zeolita (klinoptilolita) i zeolita obogaćenog amonijakom na rast i prinos crvene deteline (Trifolium pratense L.), kao i na njihovu ulogu u mikrobiološkoj dinamici zemljišta. Biljke sejane u sudovima su košene četiri puta i meren je prinos suve materije (SM) svakog otkosa. Broj bakterija, plesni, amonifikatora, Azotobacter sp. i aktinomiceta je utvrđen na početku ogleda i nakon svakog košenja. U istraživanja su uključena dva kontrolna tretmana (zemljište bez đubriva i zemljište sa dodatim mineralnim đubrivom - KAN). Primena klinoptilolita obogaćenog sa amonijakom je pozitivno i značajno uticala na mikrobiološku aktivnost i povećala je prinos (od 4,9 g po sudu kod tretmana sa klinoptilolitom obogaćenim amonijakom do 4,3 g po sudu u zemljištu sa zemljištem i zeolitom zajedno). Naša istraživanja sugerišu da se može preporučiti dodavanje klinoptilolita organskim đubrivima (stajnjaku, kompostu). Ključna dobit od primene klinoptilolita bi se ogledala u povećanju zastupljenosti mikrobioloških zajednica i njihove aktivnosti.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth
T1  - Stimulacija zemljišne mikrobiološke aktivnosti sa klinoptilolitom - uticaj na rast biljke
EP  - 123
IS  - 3
SP  - 117
VL  - 54
DO  - 10.5937/ratpov54-14870
ER  - 

@article{
author = "Karličić, Vera and Živanović, Iva and Matijašević, Danka and Raičević, Vera and Nikšić, Miomir and Rac, Vladislav and Simić, Aleksandar",
year = "2017",
abstract = "Zeolites can mitigate ammonium losses to the environment by taking up the ions from sources such as farmyard manure and ammonium-bearing fertilizers. This ability can improve nitrogen fertilizing practice given that ammonium ions loaded onto zeolite are available for plant uptake. The main goal of this study was to assess the influence of zeolite (clinoptilolite) and ammonia-loaded clinoptilolite on growth and yield of red clover (Trifolium pratense L.), as well as their influence on the microbial dynamics in soil. Plants sown in pots were cut four times, and dry matter yield (DM) of each harvest was weighed. The number of culturable bacteria, moulds, ammonifiers, Azotobacter sp. and actinomycetes was determined at the beginning of the experiment, and after each harvest. Two control treatments (soil without fertilizer and soil supplemented with mineral fertilizer - CAN) were included in the study. The application of ammonia-loaded clinoptilolite positively and significantly affected the microbial activity and provided higher yields (from 4.9 g/pot in ammonia-loaded clinoptilolite treatment to 4.3 g/pot in soil with zeolite treatment). Our results suggest that the addition of clinoptilolite to organic fertilizers (manure, composts) could be recommended. The increase of microbial communities’ abundances and their activity represented the key benefit from clinoptilolite application., Zeoliti mogu ublažiti otpuštanje amonijaka u prirodu preko uzimanja jona iz izvora kao što su stajnjak i đubriva bogata amonijakom. Ova sposobnost može unaprediti đubrenje azotom preko zeolitom usvojenih amonijum jona koji bi tako postali dostupni za usvajanje biljkama. Cilj ovih ispitivanja je bio procena uticaja zeolita (klinoptilolita) i zeolita obogaćenog amonijakom na rast i prinos crvene deteline (Trifolium pratense L.), kao i na njihovu ulogu u mikrobiološkoj dinamici zemljišta. Biljke sejane u sudovima su košene četiri puta i meren je prinos suve materije (SM) svakog otkosa. Broj bakterija, plesni, amonifikatora, Azotobacter sp. i aktinomiceta je utvrđen na početku ogleda i nakon svakog košenja. U istraživanja su uključena dva kontrolna tretmana (zemljište bez đubriva i zemljište sa dodatim mineralnim đubrivom - KAN). Primena klinoptilolita obogaćenog sa amonijakom je pozitivno i značajno uticala na mikrobiološku aktivnost i povećala je prinos (od 4,9 g po sudu kod tretmana sa klinoptilolitom obogaćenim amonijakom do 4,3 g po sudu u zemljištu sa zemljištem i zeolitom zajedno). Naša istraživanja sugerišu da se može preporučiti dodavanje klinoptilolita organskim đubrivima (stajnjaku, kompostu). Ključna dobit od primene klinoptilolita bi se ogledala u povećanju zastupljenosti mikrobioloških zajednica i njihove aktivnosti.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth, Stimulacija zemljišne mikrobiološke aktivnosti sa klinoptilolitom - uticaj na rast biljke",
pages = "123-117",
number = "3",
volume = "54",
doi = "10.5937/ratpov54-14870"
}

Karličić, V., Živanović, I., Matijašević, D., Raičević, V., Nikšić, M., Rac, V.,& Simić, A.. (2017). Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(3), 117-123.
https://doi.org/10.5937/ratpov54-14870

Karličić V, Živanović I, Matijašević D, Raičević V, Nikšić M, Rac V, Simić A. Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth. in Ratarstvo i povrtarstvo. 2017;54(3):117-123.
doi:10.5937/ratpov54-14870 .

Karličić, Vera, Živanović, Iva, Matijašević, Danka, Raičević, Vera, Nikšić, Miomir, Rac, Vladislav, Simić, Aleksandar, "Stimulation of soil microbiological activity by clinoptilolite: The effect on plant growth" in Ratarstvo i povrtarstvo, 54, no. 3 (2017):117-123,
https://doi.org/10.5937/ratpov54-14870 . .

TY  - JOUR
AU  - Marković, Z.M.
AU  - Kepić, Dejan P.
AU  - Matijašević, Danka
AU  - Pavlović, Vladimir
AU  - Jovanović, S.P.
AU  - Stanković, N.K.
AU  - Milivojević, Dušan D.
AU  - Spitalsky, Z.
AU  - Holclajtner-Antunović, Ivanka
AU  - Bajuk-Bogdanović, Danica
AU  - Nikšić, Miomir
AU  - Todorović-Marković, Biljana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4467
AB  - Curcumin and its derivates are well-known for their different biological activities including antibacterial. On the other hand there are controversial reports concerning the antibacterial potential of graphene and, in particular, graphene oxide. In this study we have reported for the first time the antibacterial activity of curcumin/graphene nanomesh hybrids under ambient light conditions. The graphene nanomesh was synthesized by electrochemical exfoliation of highly oriented pyrolytic graphite in 1 M solution of ammonium persulfate and further functionalized by curcumin. Identical values of minimum inhibitory concentration (1 mg mL(-1)) were determined for pure curcumin and curcumin/graphene nanomesh hybrids toward Staphylococcus aureus. All tested samples had more pronounced antibacterial activity against Gram positive bacteria, Staphylococcus aureus compared to Escherichia coli as a representative of Gram negative strains. The poor antibacterial potential of exfoliated graphene improves significantly by the functionalization with curcumin, which allows for its usage as a antibacterial coating.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Ambient light induced antibacterial action of curcumin/graphene nanomesh hybrids
EP  - 36092
IS  - 57
SP  - 36081
VL  - 7
DO  - 10.1039/c7ra05027e
ER  - 

@article{
author = "Marković, Z.M. and Kepić, Dejan P. and Matijašević, Danka and Pavlović, Vladimir and Jovanović, S.P. and Stanković, N.K. and Milivojević, Dušan D. and Spitalsky, Z. and Holclajtner-Antunović, Ivanka and Bajuk-Bogdanović, Danica and Nikšić, Miomir and Todorović-Marković, Biljana",
year = "2017",
abstract = "Curcumin and its derivates are well-known for their different biological activities including antibacterial. On the other hand there are controversial reports concerning the antibacterial potential of graphene and, in particular, graphene oxide. In this study we have reported for the first time the antibacterial activity of curcumin/graphene nanomesh hybrids under ambient light conditions. The graphene nanomesh was synthesized by electrochemical exfoliation of highly oriented pyrolytic graphite in 1 M solution of ammonium persulfate and further functionalized by curcumin. Identical values of minimum inhibitory concentration (1 mg mL(-1)) were determined for pure curcumin and curcumin/graphene nanomesh hybrids toward Staphylococcus aureus. All tested samples had more pronounced antibacterial activity against Gram positive bacteria, Staphylococcus aureus compared to Escherichia coli as a representative of Gram negative strains. The poor antibacterial potential of exfoliated graphene improves significantly by the functionalization with curcumin, which allows for its usage as a antibacterial coating.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Ambient light induced antibacterial action of curcumin/graphene nanomesh hybrids",
pages = "36092-36081",
number = "57",
volume = "7",
doi = "10.1039/c7ra05027e"
}

Marković, Z.M., Kepić, D. P., Matijašević, D., Pavlović, V., Jovanović, S.P., Stanković, N.K., Milivojević, D. D., Spitalsky, Z., Holclajtner-Antunović, I., Bajuk-Bogdanović, D., Nikšić, M.,& Todorović-Marković, B.. (2017). Ambient light induced antibacterial action of curcumin/graphene nanomesh hybrids. in RSC Advances
Royal Soc Chemistry, Cambridge., 7(57), 36081-36092.
https://doi.org/10.1039/c7ra05027e

Marković Z, Kepić DP, Matijašević D, Pavlović V, Jovanović S, Stanković N, Milivojević DD, Spitalsky Z, Holclajtner-Antunović I, Bajuk-Bogdanović D, Nikšić M, Todorović-Marković B. Ambient light induced antibacterial action of curcumin/graphene nanomesh hybrids. in RSC Advances. 2017;7(57):36081-36092.
doi:10.1039/c7ra05027e .

Marković, Z.M., Kepić, Dejan P., Matijašević, Danka, Pavlović, Vladimir, Jovanović, S.P., Stanković, N.K., Milivojević, Dušan D., Spitalsky, Z., Holclajtner-Antunović, Ivanka, Bajuk-Bogdanović, Danica, Nikšić, Miomir, Todorović-Marković, Biljana, "Ambient light induced antibacterial action of curcumin/graphene nanomesh hybrids" in RSC Advances, 7, no. 57 (2017):36081-36092,
https://doi.org/10.1039/c7ra05027e . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Matijašević, Danka
AU  - Pavlović, Vladimir
AU  - Jovanović, Svetlana P.
AU  - Holclajtner-Antunović, Ivanka
AU  - Spitalsky, Zdenko
AU  - Micusik, Matej
AU  - Dramicanin, Miroslav D.
AU  - Milivojević, Dušan D.
AU  - Nikšić, Miomir
AU  - Todorović-Marković, Biljana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4348
AB  - Electrochemically exfoliated graphene is functionalized graphene with potential application in biomedicine. Two most relevant biological features of this material are its electrical conductivity and excellent water dispersibility. In this study we have tried to establish the correlation between graphene structure and its antibacterial properties. The exfoliation process was performed in a two electrode-highly oriented pyrolytic graphite electrochemical cell. Solution of ammonium persulfate was used as an electrolyte. Exfoliated graphene sheets were dispersed in aqueous media and characterized by atomic force microscopy, scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy, X photoelectron spectroscopy, X-ray diffraction, electron paramagnetic resonance, zeta potential, contact angle measurements and surface energy. Antibacterial assays have shown lack of the significant antibacterial activity. Major effect on bacteria was slight change of bacteria morphology. Membrane remained intact despite significant change of chemical content of membrane components.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Colloid and Interface Science
T1  - Antibacterial potential of electrochemically exfoliated graphene sheets
EP  - 43
SP  - 30
VL  - 500
DO  - 10.1016/j.jcis.2017.03.110
ER  - 

@article{
author = "Marković, Zoran M. and Matijašević, Danka and Pavlović, Vladimir and Jovanović, Svetlana P. and Holclajtner-Antunović, Ivanka and Spitalsky, Zdenko and Micusik, Matej and Dramicanin, Miroslav D. and Milivojević, Dušan D. and Nikšić, Miomir and Todorović-Marković, Biljana",
year = "2017",
abstract = "Electrochemically exfoliated graphene is functionalized graphene with potential application in biomedicine. Two most relevant biological features of this material are its electrical conductivity and excellent water dispersibility. In this study we have tried to establish the correlation between graphene structure and its antibacterial properties. The exfoliation process was performed in a two electrode-highly oriented pyrolytic graphite electrochemical cell. Solution of ammonium persulfate was used as an electrolyte. Exfoliated graphene sheets were dispersed in aqueous media and characterized by atomic force microscopy, scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy, X photoelectron spectroscopy, X-ray diffraction, electron paramagnetic resonance, zeta potential, contact angle measurements and surface energy. Antibacterial assays have shown lack of the significant antibacterial activity. Major effect on bacteria was slight change of bacteria morphology. Membrane remained intact despite significant change of chemical content of membrane components.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Colloid and Interface Science",
title = "Antibacterial potential of electrochemically exfoliated graphene sheets",
pages = "43-30",
volume = "500",
doi = "10.1016/j.jcis.2017.03.110"
}

Marković, Z. M., Matijašević, D., Pavlović, V., Jovanović, S. P., Holclajtner-Antunović, I., Spitalsky, Z., Micusik, M., Dramicanin, M. D., Milivojević, D. D., Nikšić, M.,& Todorović-Marković, B.. (2017). Antibacterial potential of electrochemically exfoliated graphene sheets. in Journal of Colloid and Interface Science
Academic Press Inc Elsevier Science, San Diego., 500, 30-43.
https://doi.org/10.1016/j.jcis.2017.03.110

Marković ZM, Matijašević D, Pavlović V, Jovanović SP, Holclajtner-Antunović I, Spitalsky Z, Micusik M, Dramicanin MD, Milivojević DD, Nikšić M, Todorović-Marković B. Antibacterial potential of electrochemically exfoliated graphene sheets. in Journal of Colloid and Interface Science. 2017;500:30-43.
doi:10.1016/j.jcis.2017.03.110 .

Marković, Zoran M., Matijašević, Danka, Pavlović, Vladimir, Jovanović, Svetlana P., Holclajtner-Antunović, Ivanka, Spitalsky, Zdenko, Micusik, Matej, Dramicanin, Miroslav D., Milivojević, Dušan D., Nikšić, Miomir, Todorović-Marković, Biljana, "Antibacterial potential of electrochemically exfoliated graphene sheets" in Journal of Colloid and Interface Science, 500 (2017):30-43,
https://doi.org/10.1016/j.jcis.2017.03.110 . .

TY  - CONF
AU  - Pantić, Milena
AU  - Duvnjak, Dunja
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Tomasz, Słowiński
AU  - Turlo, Jadwiga
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6846
AB  - Fungal members of the class Basidiomycetes are recognized as potential sources of bioactive
compounds, which can be used in a possible replacement of the synthetic antioxidants and antibiotics
by the naturally originated ones. Genus Pleurotus includes over 50 species of edible and medicinal
mushrooms with great economic importance. Their fruiting bodies are rich in carbohydrates (28 -
50%), edible fibers (3-50%), protein (10 - 30%), essential amino acids (25-35%), vitamins (C, A, B, D),
minerals (K, P, Mg, Na, Fe, Zn) and lipids (3-5%).The aim of this research was to compare biological
characteristic of different extracts obtained from Oyster mushroom fruiting body, as well as to identify
the organic compounds responsible for detected activities and finally to summarize available data on
the factors that could affect on their antioxidant and antimicrobial activity. Samples of two types of
polysaccharide extracts, namely hot water (HW) and hot alkaline (HA) ones were prepared from
fruiting bodies of the industrially grown oyster mushroom Pleurotus ostreatus P80. Extracts were
examined for their antimicrobial and antioxidant biological activity. The antibacterial activity was the
widest against the Gram-positive bacteria Staphylococcus aureus for both types of polysaccharide
extracts. HA extract singled out as the most effective against Gram-negative bacteria Proteus hauseri
with microbicidal concentration of 2.5 mg mL–1
. HW polysaccharide extract was better antioxidant
which was shown through the lower EC50 values of DPPH scavenging ability and ferric-reducing
antioxidant power. EC50 values of the DPPH scavenging activity of the polysaccharides from HW and
HA extracts were found to be particularly low, 0.6 and 0.7 mg mL-1
, respectively. EC50 values of the
ferric-reducing antioxidant power were 4.3 and 4.5 mg mL-1 for HW and HA samples. Using
spectroscopic methods it was found that HW extracts contained more protein (399 mg g-1
) and
phenolic compounds (6.36 mg g-1
) compared to HA sample (20 mg g-1 protein and 4.96 mg g-1 phenolic
compounds). Structural FT-IR analysis also confirmed higher content of glucans in HW
polysaccharides.
PB  - University of Novi Sad Institute of Food Technology Bulevar cara Lazara 1; 21000 Novi Sad
C3  - Saopštenje sa međunarodnog skupa štampano u celini
T1  - Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain
EP  - 233
SP  - 229
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6846
ER  - 

@conference{
author = "Pantić, Milena and Duvnjak, Dunja and Matijašević, Danka and Sknepnek, Aleksandra and Tomasz, Słowiński and Turlo, Jadwiga and Nikšić, Miomir",
year = "2016",
abstract = "Fungal members of the class Basidiomycetes are recognized as potential sources of bioactive
compounds, which can be used in a possible replacement of the synthetic antioxidants and antibiotics
by the naturally originated ones. Genus Pleurotus includes over 50 species of edible and medicinal
mushrooms with great economic importance. Their fruiting bodies are rich in carbohydrates (28 -
50%), edible fibers (3-50%), protein (10 - 30%), essential amino acids (25-35%), vitamins (C, A, B, D),
minerals (K, P, Mg, Na, Fe, Zn) and lipids (3-5%).The aim of this research was to compare biological
characteristic of different extracts obtained from Oyster mushroom fruiting body, as well as to identify
the organic compounds responsible for detected activities and finally to summarize available data on
the factors that could affect on their antioxidant and antimicrobial activity. Samples of two types of
polysaccharide extracts, namely hot water (HW) and hot alkaline (HA) ones were prepared from
fruiting bodies of the industrially grown oyster mushroom Pleurotus ostreatus P80. Extracts were
examined for their antimicrobial and antioxidant biological activity. The antibacterial activity was the
widest against the Gram-positive bacteria Staphylococcus aureus for both types of polysaccharide
extracts. HA extract singled out as the most effective against Gram-negative bacteria Proteus hauseri
with microbicidal concentration of 2.5 mg mL–1
. HW polysaccharide extract was better antioxidant
which was shown through the lower EC50 values of DPPH scavenging ability and ferric-reducing
antioxidant power. EC50 values of the DPPH scavenging activity of the polysaccharides from HW and
HA extracts were found to be particularly low, 0.6 and 0.7 mg mL-1
, respectively. EC50 values of the
ferric-reducing antioxidant power were 4.3 and 4.5 mg mL-1 for HW and HA samples. Using
spectroscopic methods it was found that HW extracts contained more protein (399 mg g-1
) and
phenolic compounds (6.36 mg g-1
) compared to HA sample (20 mg g-1 protein and 4.96 mg g-1 phenolic
compounds). Structural FT-IR analysis also confirmed higher content of glucans in HW
polysaccharides.",
publisher = "University of Novi Sad Institute of Food Technology Bulevar cara Lazara 1; 21000 Novi Sad",
journal = "Saopštenje sa međunarodnog skupa štampano u celini",
title = "Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain",
pages = "233-229",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6846"
}

Pantić, M., Duvnjak, D., Matijašević, D., Sknepnek, A., Tomasz, S., Turlo, J.,& Nikšić, M.. (2016). Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain. in Saopštenje sa međunarodnog skupa štampano u celini
University of Novi Sad Institute of Food Technology Bulevar cara Lazara 1; 21000 Novi Sad., 229-233.
https://hdl.handle.net/21.15107/rcub_agrospace_6846

Pantić M, Duvnjak D, Matijašević D, Sknepnek A, Tomasz S, Turlo J, Nikšić M. Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain. in Saopštenje sa međunarodnog skupa štampano u celini. 2016;:229-233.
https://hdl.handle.net/21.15107/rcub_agrospace_6846 .

Pantić, Milena, Duvnjak, Dunja, Matijašević, Danka, Sknepnek, Aleksandra, Tomasz, Słowiński, Turlo, Jadwiga, Nikšić, Miomir, "Biological potential of polysaccharide extracts obtained from commercially grown oyster mushroom strain" in Saopštenje sa međunarodnog skupa štampano u celini (2016):229-233,
https://hdl.handle.net/21.15107/rcub_agrospace_6846 .

TY  - CONF
AU  - Duvnjak, Dunja
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Jovanović, Ljubinko
AU  - Vasiljević, Ivana
AU  - Lazović, Milana
AU  - Nikšić, Miomir
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6834
AB  - Coriolus versicolor je medicinska gljiva čija je lekovitost potvrđena
brojnim istraživanjima. Biološki aktivni polisaharidi i polisaharopeptidi
izolovani iz micelije submerzno gajene Coriolus versicolor gljive se
koriste u prevenciji mnogih bolesti. Ova gljiva se može gajiti i na čvrstom
supstratu, na otpadu iz poljoprivrede i na taj način se može uspešno
upotrebiti pri bioremedijaciji. Cilj ovog istraživanja je bio da se utvrdi da
li se antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus
versicolor gajenjem na čvrstom supstratu obogaćenom selenom može
povećati.
Kao supstrat za gajenje upotrebljena je mešavina slame, hrastove pljevine
i pšeničnih mekinja. Za obogaćivanje supstrata korišćeni su organski i
neogranski izvori selena: selesnki kvasac (Sel-Plex®
, Alltech Inc.,
Lexington, USA) i natrijum selenit, Na2SeO3 u koncentraciji od 25 mg/kg
suvog supstrata. Kontrolni uzorak je pripremljen bez dodavanja selena.
Nakon metanolne ekstrakcije, određen je sadržaj selena u digestovanim
uzorcima upotrebom ICP-OES uređaja. Kod uzorka obogaćenih selenom
iz neorganske soli sadržaj selena je bio 123,4 µg/g, a kod uzoraka
obogaćenih selenom iz selenskog kvasca 62,4 µg/g suve mase uzorka.
Mikrodilucionom metodom ispitana je antimikrobna aktivnost dobijenih
metanolnih ekstrakata na 15 sojeva bakterija i utvrđeno je da dodatak
neorganske soli dovodi do povećanja antimikrobne aktivnosti u odnosu na
kontrolni uzorak samo kod dve Gram-pozitivne bakterije: Bacillus
spizizeni ATCC 6633 gde je MIC<0,3125 mg/ml i Enerococcus faecalis
ATCC 29212, MIC=40 mg/ml. Sa druge stane, ispitivanjem
antimikrobnog delovanja ekstrakata dobijenih iz gljive obogaćene selenom iz kvasca primećeno je izraženije mikrobistatično i mikrobicidno
delovanje u poređenju i sa kontrolnim uzorkom i sa uzorkom kome je
dodat Na2SeO3 kod skoro svih testiranih Gram-pozitivnih i Gramnegativnih bakterija.
Iako dobijeni rezultati ukazuju na to da je u ekstraktu gljive koja je rasla
na supstratu sa selenskim kvascem detektovano duplo manje ukupnog
selena nego kod uzorka obogaćenog selenitom, antimikrobna aktivnost
takvih uzoraka je bila znatno izraženija. Pretpostavlja se da je selen iz
organskog izvora promenio hemijski sastav gljive.
PB  - UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd
C3  - Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije
T1  - Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.
EP  - 201
SP  - 200
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6834
ER  - 

@conference{
author = "Duvnjak, Dunja and Pantić, Milena and Matijašević, Danka and Jovanović, Ljubinko and Vasiljević, Ivana and Lazović, Milana and Nikšić, Miomir",
year = "2015",
abstract = "Coriolus versicolor je medicinska gljiva čija je lekovitost potvrđena
brojnim istraživanjima. Biološki aktivni polisaharidi i polisaharopeptidi
izolovani iz micelije submerzno gajene Coriolus versicolor gljive se
koriste u prevenciji mnogih bolesti. Ova gljiva se može gajiti i na čvrstom
supstratu, na otpadu iz poljoprivrede i na taj način se može uspešno
upotrebiti pri bioremedijaciji. Cilj ovog istraživanja je bio da se utvrdi da
li se antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus
versicolor gajenjem na čvrstom supstratu obogaćenom selenom može
povećati.
Kao supstrat za gajenje upotrebljena je mešavina slame, hrastove pljevine
i pšeničnih mekinja. Za obogaćivanje supstrata korišćeni su organski i
neogranski izvori selena: selesnki kvasac (Sel-Plex®
, Alltech Inc.,
Lexington, USA) i natrijum selenit, Na2SeO3 u koncentraciji od 25 mg/kg
suvog supstrata. Kontrolni uzorak je pripremljen bez dodavanja selena.
Nakon metanolne ekstrakcije, određen je sadržaj selena u digestovanim
uzorcima upotrebom ICP-OES uređaja. Kod uzorka obogaćenih selenom
iz neorganske soli sadržaj selena je bio 123,4 µg/g, a kod uzoraka
obogaćenih selenom iz selenskog kvasca 62,4 µg/g suve mase uzorka.
Mikrodilucionom metodom ispitana je antimikrobna aktivnost dobijenih
metanolnih ekstrakata na 15 sojeva bakterija i utvrđeno je da dodatak
neorganske soli dovodi do povećanja antimikrobne aktivnosti u odnosu na
kontrolni uzorak samo kod dve Gram-pozitivne bakterije: Bacillus
spizizeni ATCC 6633 gde je MIC<0,3125 mg/ml i Enerococcus faecalis
ATCC 29212, MIC=40 mg/ml. Sa druge stane, ispitivanjem
antimikrobnog delovanja ekstrakata dobijenih iz gljive obogaćene selenom iz kvasca primećeno je izraženije mikrobistatično i mikrobicidno
delovanje u poređenju i sa kontrolnim uzorkom i sa uzorkom kome je
dodat Na2SeO3 kod skoro svih testiranih Gram-pozitivnih i Gramnegativnih bakterija.
Iako dobijeni rezultati ukazuju na to da je u ekstraktu gljive koja je rasla
na supstratu sa selenskim kvascem detektovano duplo manje ukupnog
selena nego kod uzorka obogaćenog selenitom, antimikrobna aktivnost
takvih uzoraka je bila znatno izraženija. Pretpostavlja se da je selen iz
organskog izvora promenio hemijski sastav gljive.",
publisher = "UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd",
journal = "Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije",
title = "Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.",
pages = "201-200",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6834"
}

Duvnjak, D., Pantić, M., Matijašević, D., Jovanović, L., Vasiljević, I., Lazović, M.,& Nikšić, M.. (2015). Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.. in Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije
UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd., 200-201.
https://hdl.handle.net/21.15107/rcub_agrospace_6834

Duvnjak D, Pantić M, Matijašević D, Jovanović L, Vasiljević I, Lazović M, Nikšić M. Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena.. in Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije. 2015;:200-201.
https://hdl.handle.net/21.15107/rcub_agrospace_6834 .

Duvnjak, Dunja, Pantić, Milena, Matijašević, Danka, Jovanović, Ljubinko, Vasiljević, Ivana, Lazović, Milana, Nikšić, Miomir, "Antibakterijska aktivnost metanolnog ekstrakta gljive Coriolus versicolor obogaćene organskim i neorganskim izvorom selena." in Mikromed 2015 [Elektronski izvor] : zbornik radova / X Kongres mikrobiologa Srbije (2015):200-201,
https://hdl.handle.net/21.15107/rcub_agrospace_6834 .