Phytochemical properties of nutmeg (Myristica fragrans)
Authors
Vuković, SandraMoravčević, Đorđe
Jelačić, Slavica
Vujošević, Ana
Pećinar, Ilinka
Kilibarda, Sofija
Sudimac, Maja
Kostić, Aleksandar Ž.
Conference object (Published version)
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Show full item recordAbstract
Myristica fragrans (fam. Myristicaceae) – the source of the spice nutmeg, is evergreen tree
widespread in warm and humid tropical climate. Indonesia is the largest nutmeg world
producer. Nutmeg seeds are valuable due many uses: in cooking (spice in sweet or salt dishes),
traditional medicine (diseases of the nervous, digestive and dentistry), additive in the products
of the pharmaceutical and perfumery industry. Nutmeg seed is a good source of primary
(carbohydrates, lipids, proteins, vitamins) and secondary metabolites (terpenes, phenolics,
organic acids). The main components are lipids (30-40%) and essential oil (10-15%). Scientific
sources indicate on many pharmacological effects of nutmeg: antidepressant, hepatoprotective,
antioxidant, anticancer, etc. Climatic conditions have significant impact on flavor and
therapeutic effect of nutmeg. In this research the content of total phenolics (TPC), total
flavonoids (TFC), total hydroxycinnamic acid derivatives (THCAs) and total... carotenoids
(TCC) was determined by spectrophotometrically methods, in order to evaluate the potential
healing effect of nutmeg. Tested extracts were prepared from dried and ground nutmeg seed
commercially purchased, using two solvents (80% acetone and warm water - 50°C) and two
extraction techniques (solvent extraction (SE) and ultrasound assisted extraction (USE)). The
obtained results indicate a rich content of carotenoids and phenolics in acetone extracts.
Precisely, the TCC was 49.94 ± 0.70 μg/g in extract prepared by SE and 53.01 ± 1.28 μg/g in
extract prepared by UAE. The TPC in acetone extracts prepared by SE and UAE was similar,
28.93 ± 0.18 mg GAE/g of DW and 28.78 ± 0.07 mg GAE/g of DW, respectively, which is
statistically significantly higher than the TPC achieved in water extracts (2.97 ± 0.14 mg GAE/g
of DW for SE extract and 3.00 ± 0.21 mg GAE/g of DW for UAE extract). The TFC and THACs
achieved in acetone extracts was statistically significantly higher than TFC and THACs
observed in water extracts, where in the case of both solvents, the extraction technique does not
influence statistically significantly on the TF and THACs content. The TFC and THACs in
acetone extracts prepared by SE was 1.50 ± 0.06 mg CE/g of DW and 6.13 ± 0.08 mg CGAE/g
of DW, while acetone extracts prepared by UAE contained TF 1.59 ± 0.00 mg CE/g of DW and
THACs 5.96 ± 0.27 mg CGAE/g of DW, respectively.
Keywords:
nutmeg / phytochemical propertiesSource:
V. International Agricultural, Biological & Life Science Conference, 2023, 260-260Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
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Institution/Community
Poljoprivredni fakultetTY - CONF AU - Vuković, Sandra AU - Moravčević, Đorđe AU - Jelačić, Slavica AU - Vujošević, Ana AU - Pećinar, Ilinka AU - Kilibarda, Sofija AU - Sudimac, Maja AU - Kostić, Aleksandar Ž. PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6841 AB - Myristica fragrans (fam. Myristicaceae) – the source of the spice nutmeg, is evergreen tree widespread in warm and humid tropical climate. Indonesia is the largest nutmeg world producer. Nutmeg seeds are valuable due many uses: in cooking (spice in sweet or salt dishes), traditional medicine (diseases of the nervous, digestive and dentistry), additive in the products of the pharmaceutical and perfumery industry. Nutmeg seed is a good source of primary (carbohydrates, lipids, proteins, vitamins) and secondary metabolites (terpenes, phenolics, organic acids). The main components are lipids (30-40%) and essential oil (10-15%). Scientific sources indicate on many pharmacological effects of nutmeg: antidepressant, hepatoprotective, antioxidant, anticancer, etc. Climatic conditions have significant impact on flavor and therapeutic effect of nutmeg. In this research the content of total phenolics (TPC), total flavonoids (TFC), total hydroxycinnamic acid derivatives (THCAs) and total carotenoids (TCC) was determined by spectrophotometrically methods, in order to evaluate the potential healing effect of nutmeg. Tested extracts were prepared from dried and ground nutmeg seed commercially purchased, using two solvents (80% acetone and warm water - 50°C) and two extraction techniques (solvent extraction (SE) and ultrasound assisted extraction (USE)). The obtained results indicate a rich content of carotenoids and phenolics in acetone extracts. Precisely, the TCC was 49.94 ± 0.70 μg/g in extract prepared by SE and 53.01 ± 1.28 μg/g in extract prepared by UAE. The TPC in acetone extracts prepared by SE and UAE was similar, 28.93 ± 0.18 mg GAE/g of DW and 28.78 ± 0.07 mg GAE/g of DW, respectively, which is statistically significantly higher than the TPC achieved in water extracts (2.97 ± 0.14 mg GAE/g of DW for SE extract and 3.00 ± 0.21 mg GAE/g of DW for UAE extract). The TFC and THACs achieved in acetone extracts was statistically significantly higher than TFC and THACs observed in water extracts, where in the case of both solvents, the extraction technique does not influence statistically significantly on the TF and THACs content. The TFC and THACs in acetone extracts prepared by SE was 1.50 ± 0.06 mg CE/g of DW and 6.13 ± 0.08 mg CGAE/g of DW, while acetone extracts prepared by UAE contained TF 1.59 ± 0.00 mg CE/g of DW and THACs 5.96 ± 0.27 mg CGAE/g of DW, respectively. C3 - V. International Agricultural, Biological & Life Science Conference T1 - Phytochemical properties of nutmeg (Myristica fragrans) EP - 260 SP - 260 UR - https://hdl.handle.net/21.15107/rcub_agrospace_6841 ER -
@conference{ author = "Vuković, Sandra and Moravčević, Đorđe and Jelačić, Slavica and Vujošević, Ana and Pećinar, Ilinka and Kilibarda, Sofija and Sudimac, Maja and Kostić, Aleksandar Ž.", year = "2023", abstract = "Myristica fragrans (fam. Myristicaceae) – the source of the spice nutmeg, is evergreen tree widespread in warm and humid tropical climate. Indonesia is the largest nutmeg world producer. Nutmeg seeds are valuable due many uses: in cooking (spice in sweet or salt dishes), traditional medicine (diseases of the nervous, digestive and dentistry), additive in the products of the pharmaceutical and perfumery industry. Nutmeg seed is a good source of primary (carbohydrates, lipids, proteins, vitamins) and secondary metabolites (terpenes, phenolics, organic acids). The main components are lipids (30-40%) and essential oil (10-15%). Scientific sources indicate on many pharmacological effects of nutmeg: antidepressant, hepatoprotective, antioxidant, anticancer, etc. Climatic conditions have significant impact on flavor and therapeutic effect of nutmeg. In this research the content of total phenolics (TPC), total flavonoids (TFC), total hydroxycinnamic acid derivatives (THCAs) and total carotenoids (TCC) was determined by spectrophotometrically methods, in order to evaluate the potential healing effect of nutmeg. Tested extracts were prepared from dried and ground nutmeg seed commercially purchased, using two solvents (80% acetone and warm water - 50°C) and two extraction techniques (solvent extraction (SE) and ultrasound assisted extraction (USE)). The obtained results indicate a rich content of carotenoids and phenolics in acetone extracts. Precisely, the TCC was 49.94 ± 0.70 μg/g in extract prepared by SE and 53.01 ± 1.28 μg/g in extract prepared by UAE. The TPC in acetone extracts prepared by SE and UAE was similar, 28.93 ± 0.18 mg GAE/g of DW and 28.78 ± 0.07 mg GAE/g of DW, respectively, which is statistically significantly higher than the TPC achieved in water extracts (2.97 ± 0.14 mg GAE/g of DW for SE extract and 3.00 ± 0.21 mg GAE/g of DW for UAE extract). The TFC and THACs achieved in acetone extracts was statistically significantly higher than TFC and THACs observed in water extracts, where in the case of both solvents, the extraction technique does not influence statistically significantly on the TF and THACs content. The TFC and THACs in acetone extracts prepared by SE was 1.50 ± 0.06 mg CE/g of DW and 6.13 ± 0.08 mg CGAE/g of DW, while acetone extracts prepared by UAE contained TF 1.59 ± 0.00 mg CE/g of DW and THACs 5.96 ± 0.27 mg CGAE/g of DW, respectively.", journal = "V. International Agricultural, Biological & Life Science Conference", title = "Phytochemical properties of nutmeg (Myristica fragrans)", pages = "260-260", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6841" }
Vuković, S., Moravčević, Đ., Jelačić, S., Vujošević, A., Pećinar, I., Kilibarda, S., Sudimac, M.,& Kostić, A. Ž.. (2023). Phytochemical properties of nutmeg (Myristica fragrans). in V. International Agricultural, Biological & Life Science Conference, 260-260. https://hdl.handle.net/21.15107/rcub_agrospace_6841
Vuković S, Moravčević Đ, Jelačić S, Vujošević A, Pećinar I, Kilibarda S, Sudimac M, Kostić AŽ. Phytochemical properties of nutmeg (Myristica fragrans). in V. International Agricultural, Biological & Life Science Conference. 2023;:260-260. https://hdl.handle.net/21.15107/rcub_agrospace_6841 .
Vuković, Sandra, Moravčević, Đorđe, Jelačić, Slavica, Vujošević, Ana, Pećinar, Ilinka, Kilibarda, Sofija, Sudimac, Maja, Kostić, Aleksandar Ž., "Phytochemical properties of nutmeg (Myristica fragrans)" in V. International Agricultural, Biological & Life Science Conference (2023):260-260, https://hdl.handle.net/21.15107/rcub_agrospace_6841 .