DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
2018
Autori
Dojčilović, RadovanPajović, Jelena D.
Božanić, Dušan K.
Jović, Nataša
Pavlović, Vera P.
Pavlović, Vladimir
Lenhardt-Acković, Lea
Zeković, Ivana
Dramicanin, Miroslav D.
Kascakova, Slavka
Refregiers, Matthieu
Rasić, Goran
Vlahović, Branislav
Djoković, Vladimir
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, p...rGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.
Ključne reči:
bioimaging / graphene oxide / fluorescence / cancer / cellsIzvor:
2d Materials, 2018, 5, 4Izdavač:
- IOP Publishing Ltd, Bristol
Finansiranje / projekti:
- NSF CRESTNational Science Foundation (NSF)NSF- Directorate for Education & Human Resources (EHR) [HRD-0833184]
- NASANational Aeronautics & Space Administration (NASA) [NNX09AV07A]
- Uticaj veličine, oblika i strukture nanočestica na njihova svojstva i svojstva nanokompozita (RS-MESTD-Basic Research (BR or ON)-172056)
- Materijali redukovane dimenzionalnosti za efikasnu apsorpciju svetlosti i konverziju energije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
- Proučavanje uticaja tretiranja na dielektrične, optičke, magnetne i osobine površina kristalnih i polimernih sistema (RS-MESTD-Basic Research (BR or ON)-171029)
DOI: 10.1088/2053-1583/aad72b
ISSN: 2053-1583
WoS: 000441754800003
Scopus: 2-s2.0-85054724220
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Dojčilović, Radovan AU - Pajović, Jelena D. AU - Božanić, Dušan K. AU - Jović, Nataša AU - Pavlović, Vera P. AU - Pavlović, Vladimir AU - Lenhardt-Acković, Lea AU - Zeković, Ivana AU - Dramicanin, Miroslav D. AU - Kascakova, Slavka AU - Refregiers, Matthieu AU - Rasić, Goran AU - Vlahović, Branislav AU - Djoković, Vladimir PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4655 AB - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. PB - IOP Publishing Ltd, Bristol T2 - 2d Materials T1 - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells IS - 4 VL - 5 DO - 10.1088/2053-1583/aad72b ER -
@article{ author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša and Pavlović, Vera P. and Pavlović, Vladimir and Lenhardt-Acković, Lea and Zeković, Ivana and Dramicanin, Miroslav D. and Kascakova, Slavka and Refregiers, Matthieu and Rasić, Goran and Vlahović, Branislav and Djoković, Vladimir", year = "2018", abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.", publisher = "IOP Publishing Ltd, Bristol", journal = "2d Materials", title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells", number = "4", volume = "5", doi = "10.1088/2053-1583/aad72b" }
Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N., Pavlović, V. P., Pavlović, V., Lenhardt-Acković, L., Zeković, I., Dramicanin, M. D., Kascakova, S., Refregiers, M., Rasić, G., Vlahović, B.,& Djoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2d Materials IOP Publishing Ltd, Bristol., 5(4). https://doi.org/10.1088/2053-1583/aad72b
Dojčilović R, Pajović JD, Božanić DK, Jović N, Pavlović VP, Pavlović V, Lenhardt-Acković L, Zeković I, Dramicanin MD, Kascakova S, Refregiers M, Rasić G, Vlahović B, Djoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2d Materials. 2018;5(4). doi:10.1088/2053-1583/aad72b .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša, Pavlović, Vera P., Pavlović, Vladimir, Lenhardt-Acković, Lea, Zeković, Ivana, Dramicanin, Miroslav D., Kascakova, Slavka, Refregiers, Matthieu, Rasić, Goran, Vlahović, Branislav, Djoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2d Materials, 5, no. 4 (2018), https://doi.org/10.1088/2053-1583/aad72b . .