Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia)
Samo za registrovane korisnike
2016
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Pseudomonas aeruginosa is a globally distributed environmental bacterium, which is also a significant opportunistic pathogen of humans, animals and plants. It is considered that wide distribution of this bacterium is connected with its most significant constitutive property to form biofilms, and that this multicellular mode of growth, predominant in nature, serves as a protective mechanism against unfavourable environmental conditions. The work presented here examines the phenotypic diversity of Pseudomonas aeruginosa environmental isolates with respect to biofilm production capacity under different environmental conditions (temperature, pH, NaCl), production of virulence factors, and motility. The purpose of this work is to present the production of two quorum sensing-regulated virulence factors (rhamnolipids and pyocyanin), explore different motility tests (swimming, swarming and twitching) and discover potential relationship between assessed phenotypic features. Obtained results del...ineate environmental conditions coinciding with biofilm production and suggest a high correlation between rhamnolipid production levels and biofilm formation. Rhamnolipids affect motility competence, yet only the flagellum-mediated swimming motility has significant impact on the biofilm formation potential. Although it is challenging to demarcate a definitive, clear correlation between parameters tested, rhamnolipid content appears to serve as a link between the tested phenotypic factors.
Ključne reči:
biofilms / motility / Pseudomonas aeruginosa / pyocyanin / rhamnolipid productionIzvor:
Water Science and Technology-Water Supply, 2016, 16, 5, 1370-1377Izdavač:
- IWA Publishing, London
Finansiranje / projekti:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
- Biodiverzitet kao potencijal u ekoremedijacionim tehnologijama oštećenih ekosistema (RS-MESTD-Technological Development (TD or TR)-31080)
- Inoviranje forenzičkih metoda i njihova primena (RS-MESTD-Technological Development (TD or TR)-34019)
DOI: 10.2166/ws.2016.061
ISSN: 1606-9749
WoS: 000386701800021
Scopus: 2-s2.0-84998886433
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Vujović, Bojana AU - Teodorović, Smilja AU - Rudić, Željka AU - Božić, Mile AU - Raičević, Vera PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4052 AB - Pseudomonas aeruginosa is a globally distributed environmental bacterium, which is also a significant opportunistic pathogen of humans, animals and plants. It is considered that wide distribution of this bacterium is connected with its most significant constitutive property to form biofilms, and that this multicellular mode of growth, predominant in nature, serves as a protective mechanism against unfavourable environmental conditions. The work presented here examines the phenotypic diversity of Pseudomonas aeruginosa environmental isolates with respect to biofilm production capacity under different environmental conditions (temperature, pH, NaCl), production of virulence factors, and motility. The purpose of this work is to present the production of two quorum sensing-regulated virulence factors (rhamnolipids and pyocyanin), explore different motility tests (swimming, swarming and twitching) and discover potential relationship between assessed phenotypic features. Obtained results delineate environmental conditions coinciding with biofilm production and suggest a high correlation between rhamnolipid production levels and biofilm formation. Rhamnolipids affect motility competence, yet only the flagellum-mediated swimming motility has significant impact on the biofilm formation potential. Although it is challenging to demarcate a definitive, clear correlation between parameters tested, rhamnolipid content appears to serve as a link between the tested phenotypic factors. PB - IWA Publishing, London T2 - Water Science and Technology-Water Supply T1 - Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia) EP - 1377 IS - 5 SP - 1370 VL - 16 DO - 10.2166/ws.2016.061 ER -
@article{ author = "Vujović, Bojana and Teodorović, Smilja and Rudić, Željka and Božić, Mile and Raičević, Vera", year = "2016", abstract = "Pseudomonas aeruginosa is a globally distributed environmental bacterium, which is also a significant opportunistic pathogen of humans, animals and plants. It is considered that wide distribution of this bacterium is connected with its most significant constitutive property to form biofilms, and that this multicellular mode of growth, predominant in nature, serves as a protective mechanism against unfavourable environmental conditions. The work presented here examines the phenotypic diversity of Pseudomonas aeruginosa environmental isolates with respect to biofilm production capacity under different environmental conditions (temperature, pH, NaCl), production of virulence factors, and motility. The purpose of this work is to present the production of two quorum sensing-regulated virulence factors (rhamnolipids and pyocyanin), explore different motility tests (swimming, swarming and twitching) and discover potential relationship between assessed phenotypic features. Obtained results delineate environmental conditions coinciding with biofilm production and suggest a high correlation between rhamnolipid production levels and biofilm formation. Rhamnolipids affect motility competence, yet only the flagellum-mediated swimming motility has significant impact on the biofilm formation potential. Although it is challenging to demarcate a definitive, clear correlation between parameters tested, rhamnolipid content appears to serve as a link between the tested phenotypic factors.", publisher = "IWA Publishing, London", journal = "Water Science and Technology-Water Supply", title = "Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia)", pages = "1377-1370", number = "5", volume = "16", doi = "10.2166/ws.2016.061" }
Vujović, B., Teodorović, S., Rudić, Ž., Božić, M.,& Raičević, V.. (2016). Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia). in Water Science and Technology-Water Supply IWA Publishing, London., 16(5), 1370-1377. https://doi.org/10.2166/ws.2016.061
Vujović B, Teodorović S, Rudić Ž, Božić M, Raičević V. Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia). in Water Science and Technology-Water Supply. 2016;16(5):1370-1377. doi:10.2166/ws.2016.061 .
Vujović, Bojana, Teodorović, Smilja, Rudić, Željka, Božić, Mile, Raičević, Vera, "Phenotypic heterogeneity of Pseudomonas aeruginosa isolates in the protected nature park 'Palic' (Serbia)" in Water Science and Technology-Water Supply, 16, no. 5 (2016):1370-1377, https://doi.org/10.2166/ws.2016.061 . .