Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)
2015
Autori
Tamaš, NenadDojnov, Biljana
Margetić, Aleksandra
Vujcić, Miroslava
Špirović, Bojana
Miletić, Novica
Stević, Milan
Vujcić, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Introduction. Aphis pomi (De Geer) has developed resistance to organophosphate and carbamate insecticides, as a result of long-term application of these insecticides in conventional apple orchards. For many years, the only mechanism of resistance identified in aphids was overproduction of insecticide-detoxifying esterases. Materials and methods. Insecticide resistance of A. pomi, collected from two conventional apple orchards (localities of Radmilovac-RA and Bela Crkva-BC) and one organic apple orchard (locality of Surcin-SU), was tested by bioassays and biochemical assays. Results and discussion. Compared with LC50 values for the susceptible population (organic orchard), both populations from the conventional orchards were highly resistant to pirimicarb (234.5 and 52.9 times) and moderately resistant to dimethoate (10.7 and 9.0 times). Increased esterase activity was determined in these two resistant aphid populations. Each of them also produced one esterase isoform more than the susc...eptible population, when 1-naphthyl acetate was used as a substrate for zymographic detection; when 2-naphthyl acetate was used as a substrate, only one resistant population produced two new esterase isoforms. In one of the resistant populations acetylcholinesterase (AChE) was significantly less inhibited by pirimicarb than in the other resistant population and the susceptible population, which indicates that this population developed another resistance mechanism-Modification of AChE (MACE). Conclusion. Detoxification of insecticides by the metabolic resistance mechanism of esterase enzymes and mechanism of modification of AChE was proven in one aphid population (RA). The other population (BC) has developed only metabolic resistance (enhanced metabolism by esterases), without modification of the insecticide target site (AChE). Development of insecticide resistance was caused by long-term application of acetylcholinesterase inhibitors (organophosphates and carbamates) in these conventional orchards.
Ključne reči:
Serbia / apple / Malus domestica / Aphis pomi / pesticide resistance / pirimicarb / esterasesIzvor:
Fruits, 2015, 70, 3, 135-142Izdavač:
- Edp Sciences S A, Les Ulis Cedex A
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-MESTD-Basic Research (BR or ON)-172048)
DOI: 10.1051/fruits/2015005
ISSN: 0248-1294
WoS: 000354372100003
Scopus: 2-s2.0-84928579040
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Tamaš, Nenad AU - Dojnov, Biljana AU - Margetić, Aleksandra AU - Vujcić, Miroslava AU - Špirović, Bojana AU - Miletić, Novica AU - Stević, Milan AU - Vujcić, Zoran PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3785 AB - Introduction. Aphis pomi (De Geer) has developed resistance to organophosphate and carbamate insecticides, as a result of long-term application of these insecticides in conventional apple orchards. For many years, the only mechanism of resistance identified in aphids was overproduction of insecticide-detoxifying esterases. Materials and methods. Insecticide resistance of A. pomi, collected from two conventional apple orchards (localities of Radmilovac-RA and Bela Crkva-BC) and one organic apple orchard (locality of Surcin-SU), was tested by bioassays and biochemical assays. Results and discussion. Compared with LC50 values for the susceptible population (organic orchard), both populations from the conventional orchards were highly resistant to pirimicarb (234.5 and 52.9 times) and moderately resistant to dimethoate (10.7 and 9.0 times). Increased esterase activity was determined in these two resistant aphid populations. Each of them also produced one esterase isoform more than the susceptible population, when 1-naphthyl acetate was used as a substrate for zymographic detection; when 2-naphthyl acetate was used as a substrate, only one resistant population produced two new esterase isoforms. In one of the resistant populations acetylcholinesterase (AChE) was significantly less inhibited by pirimicarb than in the other resistant population and the susceptible population, which indicates that this population developed another resistance mechanism-Modification of AChE (MACE). Conclusion. Detoxification of insecticides by the metabolic resistance mechanism of esterase enzymes and mechanism of modification of AChE was proven in one aphid population (RA). The other population (BC) has developed only metabolic resistance (enhanced metabolism by esterases), without modification of the insecticide target site (AChE). Development of insecticide resistance was caused by long-term application of acetylcholinesterase inhibitors (organophosphates and carbamates) in these conventional orchards. PB - Edp Sciences S A, Les Ulis Cedex A T2 - Fruits T1 - Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae) EP - 142 IS - 3 SP - 135 VL - 70 DO - 10.1051/fruits/2015005 ER -
@article{ author = "Tamaš, Nenad and Dojnov, Biljana and Margetić, Aleksandra and Vujcić, Miroslava and Špirović, Bojana and Miletić, Novica and Stević, Milan and Vujcić, Zoran", year = "2015", abstract = "Introduction. Aphis pomi (De Geer) has developed resistance to organophosphate and carbamate insecticides, as a result of long-term application of these insecticides in conventional apple orchards. For many years, the only mechanism of resistance identified in aphids was overproduction of insecticide-detoxifying esterases. Materials and methods. Insecticide resistance of A. pomi, collected from two conventional apple orchards (localities of Radmilovac-RA and Bela Crkva-BC) and one organic apple orchard (locality of Surcin-SU), was tested by bioassays and biochemical assays. Results and discussion. Compared with LC50 values for the susceptible population (organic orchard), both populations from the conventional orchards were highly resistant to pirimicarb (234.5 and 52.9 times) and moderately resistant to dimethoate (10.7 and 9.0 times). Increased esterase activity was determined in these two resistant aphid populations. Each of them also produced one esterase isoform more than the susceptible population, when 1-naphthyl acetate was used as a substrate for zymographic detection; when 2-naphthyl acetate was used as a substrate, only one resistant population produced two new esterase isoforms. In one of the resistant populations acetylcholinesterase (AChE) was significantly less inhibited by pirimicarb than in the other resistant population and the susceptible population, which indicates that this population developed another resistance mechanism-Modification of AChE (MACE). Conclusion. Detoxification of insecticides by the metabolic resistance mechanism of esterase enzymes and mechanism of modification of AChE was proven in one aphid population (RA). The other population (BC) has developed only metabolic resistance (enhanced metabolism by esterases), without modification of the insecticide target site (AChE). Development of insecticide resistance was caused by long-term application of acetylcholinesterase inhibitors (organophosphates and carbamates) in these conventional orchards.", publisher = "Edp Sciences S A, Les Ulis Cedex A", journal = "Fruits", title = "Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)", pages = "142-135", number = "3", volume = "70", doi = "10.1051/fruits/2015005" }
Tamaš, N., Dojnov, B., Margetić, A., Vujcić, M., Špirović, B., Miletić, N., Stević, M.,& Vujcić, Z.. (2015). Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae). in Fruits Edp Sciences S A, Les Ulis Cedex A., 70(3), 135-142. https://doi.org/10.1051/fruits/2015005
Tamaš N, Dojnov B, Margetić A, Vujcić M, Špirović B, Miletić N, Stević M, Vujcić Z. Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae). in Fruits. 2015;70(3):135-142. doi:10.1051/fruits/2015005 .
Tamaš, Nenad, Dojnov, Biljana, Margetić, Aleksandra, Vujcić, Miroslava, Špirović, Bojana, Miletić, Novica, Stević, Milan, Vujcić, Zoran, "Resistance to common organophosphate and carbamate insecticides in Aphis pomi (Hemiptera: Aphididae)" in Fruits, 70, no. 3 (2015):135-142, https://doi.org/10.1051/fruits/2015005 . .