TY  - CONF
AU  - Madureira, Tania Vieria
AU  - Alves, Rodrigo
AU  - Lopes, Celia
AU  - Raskovic, Bozidar
AU  - Rocha, Eduardo
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6937
AB  - Three-dimensional (3D) culture models of mammalian hepatocytes have been shown to replicate in vivo liver features accurately. The use of these 3D models avoids the constraints of an in vivo assay while sustaining the morphology and functionality of the hepatocytes throughout several days in culture. The feasibility of generating hepatic spheroids from other species, including fish, seems promising but has received less attention than in mammal models. Primary brown trout hepatocyte spheroids were first obtained by our research group from juvenile fish and cultured for over 30 days under orbital shaking. Optimization assays showed that these spheroids achieved biometric, morphological and basal gene expression stability from day 12 to day 20. This multiparametric characterization allowed the selection of a temporal window of culture days, which is being explored in several assays. The 3D primary hepatocyte spheroids were exposed to estrogens (e.g., 17α-ethinylestradiol) at different concentrations. The effect of increasing temperature was also evaluated in this model by maintaining cultures at 18°C and 21°C. Data collected include microscopy analysis, target gene expression of estrogenic and lipid pathways, and cellular biochemistry. The outputs indicate that hepatic spheroids are metabolically active and respond to different hormonal and temperature stimuli. The 3D model developed from primary brown trout hepatocytes seems to be a viable alternative for studying hepatic disruptions caused by (at least) estrogenic compounds and varied temperatures.
PB  - Iberian Association of Comparative Endocrinology
C3  - XIV Congress of the Iberian Association of Comparative Endocrinology
T1  - Brown trout primary hepatocyte spheroids – characterization and applications in endocrinology and beyond
EP  - 87
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6937
ER  - 

@conference{
author = "Madureira, Tania Vieria and Alves, Rodrigo and Lopes, Celia and Raskovic, Bozidar and Rocha, Eduardo",
year = "2023",
abstract = "Three-dimensional (3D) culture models of mammalian hepatocytes have been shown to replicate in vivo liver features accurately. The use of these 3D models avoids the constraints of an in vivo assay while sustaining the morphology and functionality of the hepatocytes throughout several days in culture. The feasibility of generating hepatic spheroids from other species, including fish, seems promising but has received less attention than in mammal models. Primary brown trout hepatocyte spheroids were first obtained by our research group from juvenile fish and cultured for over 30 days under orbital shaking. Optimization assays showed that these spheroids achieved biometric, morphological and basal gene expression stability from day 12 to day 20. This multiparametric characterization allowed the selection of a temporal window of culture days, which is being explored in several assays. The 3D primary hepatocyte spheroids were exposed to estrogens (e.g., 17α-ethinylestradiol) at different concentrations. The effect of increasing temperature was also evaluated in this model by maintaining cultures at 18°C and 21°C. Data collected include microscopy analysis, target gene expression of estrogenic and lipid pathways, and cellular biochemistry. The outputs indicate that hepatic spheroids are metabolically active and respond to different hormonal and temperature stimuli. The 3D model developed from primary brown trout hepatocytes seems to be a viable alternative for studying hepatic disruptions caused by (at least) estrogenic compounds and varied temperatures.",
publisher = "Iberian Association of Comparative Endocrinology",
journal = "XIV Congress of the Iberian Association of Comparative Endocrinology",
title = "Brown trout primary hepatocyte spheroids – characterization and applications in endocrinology and beyond",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6937"
}

Madureira, T. V., Alves, R., Lopes, C., Raskovic, B.,& Rocha, E.. (2023). Brown trout primary hepatocyte spheroids – characterization and applications in endocrinology and beyond. in XIV Congress of the Iberian Association of Comparative Endocrinology
Iberian Association of Comparative Endocrinology., 87-87.
https://hdl.handle.net/21.15107/rcub_agrospace_6937

Madureira TV, Alves R, Lopes C, Raskovic B, Rocha E. Brown trout primary hepatocyte spheroids – characterization and applications in endocrinology and beyond. in XIV Congress of the Iberian Association of Comparative Endocrinology. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_agrospace_6937 .

Madureira, Tania Vieria, Alves, Rodrigo, Lopes, Celia, Raskovic, Bozidar, Rocha, Eduardo, "Brown trout primary hepatocyte spheroids – characterization and applications in endocrinology and beyond" in XIV Congress of the Iberian Association of Comparative Endocrinology (2023):87-87,
https://hdl.handle.net/21.15107/rcub_agrospace_6937 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6354
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Ecotoxicology
T2  - Ecotoxicology
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
DO  - 10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić-Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Ecotoxicology, Ecotoxicology",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
doi = "10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić-Jeftić, Ž., Rašković, B., Poleksić, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology.
https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić-Jeftić Ž, Rašković B, Poleksić V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology. 2023;.
doi:10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić-Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Ecotoxicology (2023),
https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Poleksić, Vesna
AU  - Tasić, Aleksandra
AU  - Smederevac-Lalić, Marija
AU  - Djikanović, Vesna
AU  - Rašković, Božidar
PY  - 2023
UR  - https://econpapers.repec.org/article/gamjsusta/v_3a15_3ay_3a2023_3ai_3a14_3ap_3a11321-_3ad_3a1198759.htm
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6415
AB  - Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia) to analyze their bioindicator potential and compare their possible differences. Concentrations of 26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS). Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health. Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some 4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in both age classes. Histopathological changes and indices were minor and did not differ significantly between age classes, suggesting that pollution did not affect the morphology and structure of gills and liver. There were no significant correlations between elemental accumulation and fish condition or between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.
T2  - Sustainability
T2  - Sustainability
T1  - Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution
EP  - 13
IS  - 14
SP  - 1
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6415
ER  - 

@article{
author = "Nikolić, Dušan and Poleksić, Vesna and Tasić, Aleksandra and Smederevac-Lalić, Marija and Djikanović, Vesna and Rašković, Božidar",
year = "2023",
abstract = "Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia) to analyze their bioindicator potential and compare their possible differences. Concentrations of 26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS). Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health. Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some 4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in both age classes. Histopathological changes and indices were minor and did not differ significantly between age classes, suggesting that pollution did not affect the morphology and structure of gills and liver. There were no significant correlations between elemental accumulation and fish condition or between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.",
journal = "Sustainability, Sustainability",
title = "Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution",
pages = "13-1",
number = "14",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6415"
}

Nikolić, D., Poleksić, V., Tasić, A., Smederevac-Lalić, M., Djikanović, V.,& Rašković, B.. (2023). Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution. in Sustainability, 15(14), 1-13.
https://hdl.handle.net/21.15107/rcub_agrospace_6415

Nikolić D, Poleksić V, Tasić A, Smederevac-Lalić M, Djikanović V, Rašković B. Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution. in Sustainability. 2023;15(14):1-13.
https://hdl.handle.net/21.15107/rcub_agrospace_6415 .

Nikolić, Dušan, Poleksić, Vesna, Tasić, Aleksandra, Smederevac-Lalić, Marija, Djikanović, Vesna, Rašković, Božidar, "Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution" in Sustainability, 15, no. 14 (2023):1-13,
https://hdl.handle.net/21.15107/rcub_agrospace_6415 .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Poleksić, Vesna
AU  - Skoric, Stefan
AU  - Tasic, Aleksandra
AU  - Stanojevic, Slobodan
AU  - Raskovic, Bozidar
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6942
AB  - Five reservoirs (Vlasina, Medjuvršje, Zaovine, Perućac, and Garaši) in Serbia were chosen as study sites, which differ by their position, purpose, stages of eutrophication, management policies, and levels of anthropogenic pressure. The objectives of this research were to: determine the concentrations of 26 elements in muscle, gills, and liver of the European chub by inductively-coupled plasma optical emission spectrometry (ICP-OES); determine the concentrations of 17 organochlorine pesticides in fish muscle by gas chromatography with mass spectrometric detection (GC-MS); compare these findings with condition factor (CF) and histopathological (HP) biomarkers; and assess the potential human health risks due to consumption of chub muscle tissue. The highest elemental accumulation was found in the gills. The European chub was not a good indicator of Pb pollution between reservoirs. Concentrations of Hg, As, and Cu were low and did not exceed the proscribed maximum allowed concentrations (MACs). 4,4′-DDE was detected only in individuals from Vlasina, 4,4′-DDD from Perućac and Zaovine, and heptachlor from Zaovine. Low to moderate levels of HP were observed for both gills and liver in all studied reservoirs. HP index for gills was significantly higher for Zaovine compared to Vlasina. Significantly lower HP index for liver and the total HP index value were observed for fish from Vlasina compared to Perućac. No significant human health risks due to the intake of examined pollutants in each reservoir were recorded; women were at higher risk compared to men. A reason for concern is a few muscle samples from Garaši, Vlasina, Perućac, and Medjuvršje in which Cd exceeded the MAC. A reservoir for water supply (Garaši) is generally considered the safest for fish consumption.
PB  - Elsevier B.V.
T2  - Environmental Pollution
T1  - The European Chub (Squalius cephalus) as an indicator of reservoirs pollution and human health risk assessment associated with its consumption
SP  - 119871
VL  - 310
DO  - 10.1016/j.envpol.2022.119871
ER  - 

@article{
author = "Nikolić, Dušan and Poleksić, Vesna and Skoric, Stefan and Tasic, Aleksandra and Stanojevic, Slobodan and Raskovic, Bozidar",
year = "2022",
abstract = "Five reservoirs (Vlasina, Medjuvršje, Zaovine, Perućac, and Garaši) in Serbia were chosen as study sites, which differ by their position, purpose, stages of eutrophication, management policies, and levels of anthropogenic pressure. The objectives of this research were to: determine the concentrations of 26 elements in muscle, gills, and liver of the European chub by inductively-coupled plasma optical emission spectrometry (ICP-OES); determine the concentrations of 17 organochlorine pesticides in fish muscle by gas chromatography with mass spectrometric detection (GC-MS); compare these findings with condition factor (CF) and histopathological (HP) biomarkers; and assess the potential human health risks due to consumption of chub muscle tissue. The highest elemental accumulation was found in the gills. The European chub was not a good indicator of Pb pollution between reservoirs. Concentrations of Hg, As, and Cu were low and did not exceed the proscribed maximum allowed concentrations (MACs). 4,4′-DDE was detected only in individuals from Vlasina, 4,4′-DDD from Perućac and Zaovine, and heptachlor from Zaovine. Low to moderate levels of HP were observed for both gills and liver in all studied reservoirs. HP index for gills was significantly higher for Zaovine compared to Vlasina. Significantly lower HP index for liver and the total HP index value were observed for fish from Vlasina compared to Perućac. No significant human health risks due to the intake of examined pollutants in each reservoir were recorded; women were at higher risk compared to men. A reason for concern is a few muscle samples from Garaši, Vlasina, Perućac, and Medjuvršje in which Cd exceeded the MAC. A reservoir for water supply (Garaši) is generally considered the safest for fish consumption.",
publisher = "Elsevier B.V.",
journal = "Environmental Pollution",
title = "The European Chub (Squalius cephalus) as an indicator of reservoirs pollution and human health risk assessment associated with its consumption",
pages = "119871",
volume = "310",
doi = "10.1016/j.envpol.2022.119871"
}

Nikolić, D., Poleksić, V., Skoric, S., Tasic, A., Stanojevic, S.,& Raskovic, B.. (2022). The European Chub (Squalius cephalus) as an indicator of reservoirs pollution and human health risk assessment associated with its consumption. in Environmental Pollution
Elsevier B.V.., 310, 119871.
https://doi.org/10.1016/j.envpol.2022.119871

Nikolić D, Poleksić V, Skoric S, Tasic A, Stanojevic S, Raskovic B. The European Chub (Squalius cephalus) as an indicator of reservoirs pollution and human health risk assessment associated with its consumption. in Environmental Pollution. 2022;310:119871.
doi:10.1016/j.envpol.2022.119871 .

Nikolić, Dušan, Poleksić, Vesna, Skoric, Stefan, Tasic, Aleksandra, Stanojevic, Slobodan, Raskovic, Bozidar, "The European Chub (Squalius cephalus) as an indicator of reservoirs pollution and human health risk assessment associated with its consumption" in Environmental Pollution, 310 (2022):119871,
https://doi.org/10.1016/j.envpol.2022.119871 . .

TY  - GEN
AU  - Ehrlich, Tomke
AU  - Rinderknecht, Maximilian
AU  - Gölz, Lisa
AU  - Raskovic, Bozidar
AU  - Braunbeck, Thomas
AU  - Baumann, Lisa Annie
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6935
AB  - Currently, endocrine disruptor (ED) testing is conducted with multiple protocols using various species both for human health and environmental safety evaluation. ERGO, as part of the EU H2020 EURION cluster project, is focused on the optimisation of such existing test guidelines (TGs)for screening for ED effects of chemicals. Modified or new TGs are expected to both comply with the reduction, replacement, and refinement (3R) principle in animal testing and to reduce the time and costs needed for the evaluation and authorisation of new and existing compounds. The Hypothalamus-Pituitary-Thyroid (HPT) axis as a crucial component of homeostasis and developmental processes is preserved across vertebrate classes. Except for thyroid hormone system disruption (THSD), zebrafish (Danio rerio) has become a well-established model in ED testing. In order to optimise current testing procedures, the addition of new thyroid-related endpoints to existing TGs with for zebrafish is (over)due. From mammalian and amphibian studies, it is known that the morphology of the thyroid follicular epithelium is directly affected by thyroid hormone system-disrupting chemicals (THSDCs) as a response to interference with TH homeostasis. Consequently, histopathological analyses of thyroid follicles appear mandatory also in tests with fish. To check for the sensitivity of thyroid histopathology in fish, two model compounds with known THSD properties, iopanoic acid (IOP) and potassium perchlorate (PCL), were used to identify effects on thyroid follicle morphology in a fish early-life stage toxicity test (FELS, OECD TG 210). In order to further increase the ecological relevance, an additional recovery phase of 30 d was added after the initial 34 d exposure period. Furthermore, a histopathological analysis of the gonads is currently being carried out to assess crosstalk with the HPG axis. With regard to the target organ, the thyroid, PCL and IOP exposure had no statistically significant effects on thyroid follicle morphology, when measured at 34 dpf. After 64 d however, statistically significant differences in thyroid epithelium thickness were observed in exposure and recovery groups for both substances. The results show that implementation of thyroid epithelium thickness into TG 210 is technically feasible, However, refinement is needed to, e.g., replace amphibian testing. Moreover, life-stage sensitivity and general developmental effects must be considered when analysing THSD in TG 210.
PB  - Society of Environmental Toxicology and Chemistry Europe
T2  - SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”
T1  - Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 1: Thyroid Follicle and Gonad Histopathology
EP  - 61
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6935
ER  - 

@misc{
author = "Ehrlich, Tomke and Rinderknecht, Maximilian and Gölz, Lisa and Raskovic, Bozidar and Braunbeck, Thomas and Baumann, Lisa Annie",
year = "2022",
abstract = "Currently, endocrine disruptor (ED) testing is conducted with multiple protocols using various species both for human health and environmental safety evaluation. ERGO, as part of the EU H2020 EURION cluster project, is focused on the optimisation of such existing test guidelines (TGs)for screening for ED effects of chemicals. Modified or new TGs are expected to both comply with the reduction, replacement, and refinement (3R) principle in animal testing and to reduce the time and costs needed for the evaluation and authorisation of new and existing compounds. The Hypothalamus-Pituitary-Thyroid (HPT) axis as a crucial component of homeostasis and developmental processes is preserved across vertebrate classes. Except for thyroid hormone system disruption (THSD), zebrafish (Danio rerio) has become a well-established model in ED testing. In order to optimise current testing procedures, the addition of new thyroid-related endpoints to existing TGs with for zebrafish is (over)due. From mammalian and amphibian studies, it is known that the morphology of the thyroid follicular epithelium is directly affected by thyroid hormone system-disrupting chemicals (THSDCs) as a response to interference with TH homeostasis. Consequently, histopathological analyses of thyroid follicles appear mandatory also in tests with fish. To check for the sensitivity of thyroid histopathology in fish, two model compounds with known THSD properties, iopanoic acid (IOP) and potassium perchlorate (PCL), were used to identify effects on thyroid follicle morphology in a fish early-life stage toxicity test (FELS, OECD TG 210). In order to further increase the ecological relevance, an additional recovery phase of 30 d was added after the initial 34 d exposure period. Furthermore, a histopathological analysis of the gonads is currently being carried out to assess crosstalk with the HPG axis. With regard to the target organ, the thyroid, PCL and IOP exposure had no statistically significant effects on thyroid follicle morphology, when measured at 34 dpf. After 64 d however, statistically significant differences in thyroid epithelium thickness were observed in exposure and recovery groups for both substances. The results show that implementation of thyroid epithelium thickness into TG 210 is technically feasible, However, refinement is needed to, e.g., replace amphibian testing. Moreover, life-stage sensitivity and general developmental effects must be considered when analysing THSD in TG 210.",
publisher = "Society of Environmental Toxicology and Chemistry Europe",
journal = "SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”",
title = "Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 1: Thyroid Follicle and Gonad Histopathology",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6935"
}

Ehrlich, T., Rinderknecht, M., Gölz, L., Raskovic, B., Braunbeck, T.,& Baumann, L. A.. (2022). Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 1: Thyroid Follicle and Gonad Histopathology. in SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”
Society of Environmental Toxicology and Chemistry Europe., 61-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6935

Ehrlich T, Rinderknecht M, Gölz L, Raskovic B, Braunbeck T, Baumann LA. Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 1: Thyroid Follicle and Gonad Histopathology. in SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”. 2022;:61-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6935 .

Ehrlich, Tomke, Rinderknecht, Maximilian, Gölz, Lisa, Raskovic, Bozidar, Braunbeck, Thomas, Baumann, Lisa Annie, "Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 1: Thyroid Follicle and Gonad Histopathology" in SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society” (2022):61-61,
https://hdl.handle.net/21.15107/rcub_agrospace_6935 .

TY  - GEN
AU  - Rinderknecht, Maximilian
AU  - Koegst, Johannes
AU  - Ehrlich, Tomke
AU  - Gölz, Lisa
AU  - Raskovic, Bozidar
AU  - Braunbeck, Thomas
AU  - Baumann, Lisa Annie
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6934
AB  - Currently, both for human health and environmental safety evaluation, endocrine disruptor (ED) testing requires multiple studies across different species. The EU H2020 project “ERGO” is focused on the optimisation of existing test guidelines (TGs) relevant to endocrine activity and adversity for human and environmental health. New TGs would be beneficial to the 3R (replace, reduce, refine) principles and would also reduce time and costs for ED testing. The Hypothalamus-Pituitary-Thyroid axis (HPT) is highly conserved and plays important roles in both early development and homeostasis across vertebrate classes. Except for thyroid hormone system disruption (THSD), zebrafish (Danio rerio) is already commonly used as a model species in ED assessment. In order to expand existing TGs to allow for a comprehensive assessment using a single species only, new thyroid-related endpoints have to be evaluated in different developmental stages. Given that eye development is directly regulated by thyroid hormones (THs), possible endpoints include eye malformations. For this purpose, a fish early life-stage toxicity test (FELS, OECD TG 210) with iopanoic acid (IOP) and potassium perchlorate (PCL) was conducted. These substances were selected for their known effects on the HPT axis with different modes of action. In addition to the 34-d exposure phase, a recovery phase of 30 d was added to
determine the potential to recover from adverse effects on eye development. We assessed the eye-to-body-ratio, the thickness of the inner plexiform layer (IPL), the retinal pigment epithelium (RPE) and the photoreceptor layer (PL) as well as the ratio of the outer segment (OS of the PRL to the total PRL thickness). These endpoints have previously been shown to be sensitive to
disruption of the HPT in zebrafish embryos. Results show that eye malformations also manifest in later life-stages but tend to be reversible after recovery in clean water. Significant differences were observed for the RPE and the IPL at both time points with the OS/PRL ratio showing significant effects at 64 d. These results support the inclusion of the eye development, especially the thickness of the RPE, as a thyroid-sensitive endpoint into TG 210, thus allowing for a comprehensive assessment of ED effects in a single species. This would help reduce the number of amphibian studies required. TH level measurements are ongoing and will provide mechanistic data for our understanding of effects observed at apical level.
PB  - Society of Environmental Toxicology and Chemistry Europe
T2  - SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”
T1  - Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life-Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 2: Eye Development and Thyroid Hormones
EP  - 61
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6934
ER  - 

@misc{
author = "Rinderknecht, Maximilian and Koegst, Johannes and Ehrlich, Tomke and Gölz, Lisa and Raskovic, Bozidar and Braunbeck, Thomas and Baumann, Lisa Annie",
year = "2022",
abstract = "Currently, both for human health and environmental safety evaluation, endocrine disruptor (ED) testing requires multiple studies across different species. The EU H2020 project “ERGO” is focused on the optimisation of existing test guidelines (TGs) relevant to endocrine activity and adversity for human and environmental health. New TGs would be beneficial to the 3R (replace, reduce, refine) principles and would also reduce time and costs for ED testing. The Hypothalamus-Pituitary-Thyroid axis (HPT) is highly conserved and plays important roles in both early development and homeostasis across vertebrate classes. Except for thyroid hormone system disruption (THSD), zebrafish (Danio rerio) is already commonly used as a model species in ED assessment. In order to expand existing TGs to allow for a comprehensive assessment using a single species only, new thyroid-related endpoints have to be evaluated in different developmental stages. Given that eye development is directly regulated by thyroid hormones (THs), possible endpoints include eye malformations. For this purpose, a fish early life-stage toxicity test (FELS, OECD TG 210) with iopanoic acid (IOP) and potassium perchlorate (PCL) was conducted. These substances were selected for their known effects on the HPT axis with different modes of action. In addition to the 34-d exposure phase, a recovery phase of 30 d was added to
determine the potential to recover from adverse effects on eye development. We assessed the eye-to-body-ratio, the thickness of the inner plexiform layer (IPL), the retinal pigment epithelium (RPE) and the photoreceptor layer (PL) as well as the ratio of the outer segment (OS of the PRL to the total PRL thickness). These endpoints have previously been shown to be sensitive to
disruption of the HPT in zebrafish embryos. Results show that eye malformations also manifest in later life-stages but tend to be reversible after recovery in clean water. Significant differences were observed for the RPE and the IPL at both time points with the OS/PRL ratio showing significant effects at 64 d. These results support the inclusion of the eye development, especially the thickness of the RPE, as a thyroid-sensitive endpoint into TG 210, thus allowing for a comprehensive assessment of ED effects in a single species. This would help reduce the number of amphibian studies required. TH level measurements are ongoing and will provide mechanistic data for our understanding of effects observed at apical level.",
publisher = "Society of Environmental Toxicology and Chemistry Europe",
journal = "SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”",
title = "Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life-Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 2: Eye Development and Thyroid Hormones",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6934"
}

Rinderknecht, M., Koegst, J., Ehrlich, T., Gölz, L., Raskovic, B., Braunbeck, T.,& Baumann, L. A.. (2022). Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life-Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 2: Eye Development and Thyroid Hormones. in SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”
Society of Environmental Toxicology and Chemistry Europe., 61-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6934

Rinderknecht M, Koegst J, Ehrlich T, Gölz L, Raskovic B, Braunbeck T, Baumann LA. Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life-Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 2: Eye Development and Thyroid Hormones. in SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society”. 2022;:61-61.
https://hdl.handle.net/21.15107/rcub_agrospace_6934 .

Rinderknecht, Maximilian, Koegst, Johannes, Ehrlich, Tomke, Gölz, Lisa, Raskovic, Bozidar, Braunbeck, Thomas, Baumann, Lisa Annie, "Implementation of Thyroid-Sensitive Endpoints Into the Fish Early Life-Stage Test (FELS, OECD TG 210) With Zebrafish (Danio rerio) - Part 2: Eye Development and Thyroid Hormones" in SETAC Europe 32nd Annual Meeting “Towards a reduced pollution society” (2022):61-61,
https://hdl.handle.net/21.15107/rcub_agrospace_6934 .

TY  - GEN
AU  - Lopes, Celia
AU  - Teixeira, Ingrid
AU  - Raskovic, Bozidar
AU  - Rocha, Eduardo
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6939
AB  - Global warming is an undeniable reality, and one consequence is a continuous increase in the temperature of different water bodies. This phenomenon affects numerous processes in the aquatic ecosystems, including the impacts of the widely present endocrine-disrupting chemicals (EDCs). Within EDCs, ethinylestradiol (EE2) and progestins, such as levonorgestrel (LEV), are commonly detected in environmental waters. It is thus imperative to study the effects of temperature changes in the toxicology of EDCs to aquatic animals, including fish. Endocrine disruption research often relies on in vitro models, complying with the 3Rs principles. Primary hepatocytes are commonly used but also are specific and well-characterized cell lines, such as the rainbow trout liver-derived RTL-W1. This cell line presents several functional features of differentiated hepatocytes. To test the cell line potential to study the way temperature models the effects of EDCs, we exposed RTL-W1 cells (72h) to EE2, LEV or a mixture of both synthetic hormones (at 10 μM). Exposures were carried out at 18 ºC and 21 ºC, in 24-well plates, 80.000 cells per well. The cells grew at either temperature. At the end of exposures, cells were trypsinized, and the viability measured using the trypan blue exclusion assay. Cell suspensions were then centrifuged at 200 rcf, for 5 min, and pellets were frozen at -80 ºC for gene expression analysis. No differences were found in cell viability among experimental groups. The expression of two CYP P450 enzymes enrolled in xenobiotic metabolism was analyzed by qRT-PCR. The Two-way ANOVA results revealed that CYP1A was affected by temperature as its expression was lower at 18ºC than at 21ºC. As to CYP3A27 expression, it was independent of temperature but was significantly higher in the EE2 + LEV mixture compared with control and solvent control conditions. Given these results, the RTL-W1 cell line seems like a promising model to study the interactions of temperature and exposure effects of EDCs. Studies are undergoing to include genes from other relevant signalling pathways such as estrogenic, progestogenic and lipid metabolism.
PB  - European Society for Comparative Endocrinology and International Society for Fish Endocrinology
T2  - 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology
T1  - Testing the trout liver RTL-W1 cell line potential to study the influences of temperature on the effects of endocrine disruptors
EP  - 202
SP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6939
ER  - 

@misc{
author = "Lopes, Celia and Teixeira, Ingrid and Raskovic, Bozidar and Rocha, Eduardo",
year = "2022",
abstract = "Global warming is an undeniable reality, and one consequence is a continuous increase in the temperature of different water bodies. This phenomenon affects numerous processes in the aquatic ecosystems, including the impacts of the widely present endocrine-disrupting chemicals (EDCs). Within EDCs, ethinylestradiol (EE2) and progestins, such as levonorgestrel (LEV), are commonly detected in environmental waters. It is thus imperative to study the effects of temperature changes in the toxicology of EDCs to aquatic animals, including fish. Endocrine disruption research often relies on in vitro models, complying with the 3Rs principles. Primary hepatocytes are commonly used but also are specific and well-characterized cell lines, such as the rainbow trout liver-derived RTL-W1. This cell line presents several functional features of differentiated hepatocytes. To test the cell line potential to study the way temperature models the effects of EDCs, we exposed RTL-W1 cells (72h) to EE2, LEV or a mixture of both synthetic hormones (at 10 μM). Exposures were carried out at 18 ºC and 21 ºC, in 24-well plates, 80.000 cells per well. The cells grew at either temperature. At the end of exposures, cells were trypsinized, and the viability measured using the trypan blue exclusion assay. Cell suspensions were then centrifuged at 200 rcf, for 5 min, and pellets were frozen at -80 ºC for gene expression analysis. No differences were found in cell viability among experimental groups. The expression of two CYP P450 enzymes enrolled in xenobiotic metabolism was analyzed by qRT-PCR. The Two-way ANOVA results revealed that CYP1A was affected by temperature as its expression was lower at 18ºC than at 21ºC. As to CYP3A27 expression, it was independent of temperature but was significantly higher in the EE2 + LEV mixture compared with control and solvent control conditions. Given these results, the RTL-W1 cell line seems like a promising model to study the interactions of temperature and exposure effects of EDCs. Studies are undergoing to include genes from other relevant signalling pathways such as estrogenic, progestogenic and lipid metabolism.",
publisher = "European Society for Comparative Endocrinology and International Society for Fish Endocrinology",
journal = "30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology",
title = "Testing the trout liver RTL-W1 cell line potential to study the influences of temperature on the effects of endocrine disruptors",
pages = "202-202",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6939"
}

Lopes, C., Teixeira, I., Raskovic, B.,& Rocha, E.. (2022). Testing the trout liver RTL-W1 cell line potential to study the influences of temperature on the effects of endocrine disruptors. in 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology
European Society for Comparative Endocrinology and International Society for Fish Endocrinology., 202-202.
https://hdl.handle.net/21.15107/rcub_agrospace_6939

Lopes C, Teixeira I, Raskovic B, Rocha E. Testing the trout liver RTL-W1 cell line potential to study the influences of temperature on the effects of endocrine disruptors. in 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology. 2022;:202-202.
https://hdl.handle.net/21.15107/rcub_agrospace_6939 .

Lopes, Celia, Teixeira, Ingrid, Raskovic, Bozidar, Rocha, Eduardo, "Testing the trout liver RTL-W1 cell line potential to study the influences of temperature on the effects of endocrine disruptors" in 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology (2022):202-202,
https://hdl.handle.net/21.15107/rcub_agrospace_6939 .

TY  - GEN
AU  - Raskovic, Bozidar
AU  - Madureira, Tania Vieria
AU  - Lopes, Celia
AU  - Rocha, Eduardo
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6940
AB  - Progestins are classified as emerging micropollutants in freshwater ecosystems. The concentrations of this class of contaminants in water have been increasing, since they are of anthropogenic origin and their use in human and veterinary medicine is in expansion. Apart from well-studied effects of progestins on fish, such as alterations of behavioral processes, impaired reproduction and involvement in intersex, the effects on structure and metabolism of liver are understudied; namely when progestins and estrogens are concurrent. Thus, this study is exploring sub-chronic effects of in vitro exposure hepatocyte spheroids to progestins and 17α-ethinylestradiol. Primary hepatocytes were isolated from brown trout (n = 3) and cultured for 12 days in non-adhesive plates which were constantly agitated with frequency of 60 times per minute at 18 °C. Shortly after plating, hepatocytes started to spontaneously form spheroids (3D cellular aggregates), which increased their size over time. At day 12, spheroids were exposed to either 17α-ethinylestradiol (0.3 μM), levonorgestrel and megestrol acetate in two concentrations (0.3 and 0.6 μM) or binary mixtures of these chemicals in 0.1% ethanol as a solvent. After a 6 days exposure, spheroids were measured and sampled for biochemical (lactate dehydrogenase and resazurin assays), biometrical, immunohistochemical and gene expression analyses. Both biochemical assays did not show differences between exposed groups or between exposed and control and solvent control groups, which supported that viability of exposed spheroids was not compromised. For biometry, no significant differences in sphericity, area and diameter of spheroids were found between groups. However, when anti-vitellogenin antibody was applied on histological sections, spheroids from all exposed groups had increased expression comparing with controls, proving all three chemicals could modify the hepatocyte function. Besides estrogenic targets, genes related with lipid-metabolism pathways are under study. As a perspective, analysis of the modeling effect of temperature in the same targets is being considered.
PB  - European Society for Comparative Endocrinology and International Society for Fish Endocrinology
T2  - 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology
T1  - The effects of two progestins and 17α-ethinylestradiol on cultured hepatocyte spheroids of brown trout (salmo trutta)
EP  - 203
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6940
ER  - 

@misc{
author = "Raskovic, Bozidar and Madureira, Tania Vieria and Lopes, Celia and Rocha, Eduardo",
year = "2022",
abstract = "Progestins are classified as emerging micropollutants in freshwater ecosystems. The concentrations of this class of contaminants in water have been increasing, since they are of anthropogenic origin and their use in human and veterinary medicine is in expansion. Apart from well-studied effects of progestins on fish, such as alterations of behavioral processes, impaired reproduction and involvement in intersex, the effects on structure and metabolism of liver are understudied; namely when progestins and estrogens are concurrent. Thus, this study is exploring sub-chronic effects of in vitro exposure hepatocyte spheroids to progestins and 17α-ethinylestradiol. Primary hepatocytes were isolated from brown trout (n = 3) and cultured for 12 days in non-adhesive plates which were constantly agitated with frequency of 60 times per minute at 18 °C. Shortly after plating, hepatocytes started to spontaneously form spheroids (3D cellular aggregates), which increased their size over time. At day 12, spheroids were exposed to either 17α-ethinylestradiol (0.3 μM), levonorgestrel and megestrol acetate in two concentrations (0.3 and 0.6 μM) or binary mixtures of these chemicals in 0.1% ethanol as a solvent. After a 6 days exposure, spheroids were measured and sampled for biochemical (lactate dehydrogenase and resazurin assays), biometrical, immunohistochemical and gene expression analyses. Both biochemical assays did not show differences between exposed groups or between exposed and control and solvent control groups, which supported that viability of exposed spheroids was not compromised. For biometry, no significant differences in sphericity, area and diameter of spheroids were found between groups. However, when anti-vitellogenin antibody was applied on histological sections, spheroids from all exposed groups had increased expression comparing with controls, proving all three chemicals could modify the hepatocyte function. Besides estrogenic targets, genes related with lipid-metabolism pathways are under study. As a perspective, analysis of the modeling effect of temperature in the same targets is being considered.",
publisher = "European Society for Comparative Endocrinology and International Society for Fish Endocrinology",
journal = "30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology",
title = "The effects of two progestins and 17α-ethinylestradiol on cultured hepatocyte spheroids of brown trout (salmo trutta)",
pages = "203-203",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6940"
}

Raskovic, B., Madureira, T. V., Lopes, C.,& Rocha, E.. (2022). The effects of two progestins and 17α-ethinylestradiol on cultured hepatocyte spheroids of brown trout (salmo trutta). in 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology
European Society for Comparative Endocrinology and International Society for Fish Endocrinology., 203-203.
https://hdl.handle.net/21.15107/rcub_agrospace_6940

Raskovic B, Madureira TV, Lopes C, Rocha E. The effects of two progestins and 17α-ethinylestradiol on cultured hepatocyte spheroids of brown trout (salmo trutta). in 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology. 2022;:203-203.
https://hdl.handle.net/21.15107/rcub_agrospace_6940 .

Raskovic, Bozidar, Madureira, Tania Vieria, Lopes, Celia, Rocha, Eduardo, "The effects of two progestins and 17α-ethinylestradiol on cultured hepatocyte spheroids of brown trout (salmo trutta)" in 30th CECE & 9th ISFE Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology (2022):203-203,
https://hdl.handle.net/21.15107/rcub_agrospace_6940 .

TY  - GEN
AU  - Raskovic, Bozidar
AU  - Madureira, Tania Vieria
AU  - Lopes, Celia
AU  - Rocha, Eduardo
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6941
AB  - In 2018, approximately 10.5 million experimental animals were used in the EU for various testing purposes, and 26% of that number accounted for fish. The scientific community, regulatory agencies, and national and EU policymakers endorse the refinement, reduction, and replacement (3Rs) of assays to reduce the number of sacrificed experimental animals. Despite the guidelines in fish exposure assays being well established (such as OECD and US EPA) and the number of test animals kept as low as feasible, each exposure study still requires at least 105 fish. For this reason, many in vitro methods were developed or improved for use in fish assays, which provided a good platform for testing the effects of chemicals, complementing, or replacing in vivo studies. Methods from mammalian in vitro testing were used to develop primary piscine cultures and cell lines, typically cultured in monolayers, viz. two-dimensional (2D). The advantages of 2D cultures are rapid exposure tests, being easy for handling and requiring low-cost maintenance. However, the uses of 2D cultures also have downsides: cells cannot last for a long time and cannot mimic the organization of tissues since they are cultured in the bottom of flasks and plates. To solve these problems, researchers created three-dimensional (3D) cultures. The microarchitecture and physiology of cells in 3D cultures are closer to in vivo systems. 3D primary cultures in fish have been seldom used, but they may provide an excellent research tool for the toxicological assessment of chemicals. To further use fish spheroids in toxicology, we are expanding a protocol for routine isolation and culture/co-culture of cells from brown trout (Salmo trutta) liver. The protocol is compatible with exploring differential centrifugation of isolated cells to change the composition of spheroids in terms of the ratio of hepatocytes and biliary epithelial cells. After plating in non-adhesive plates, cells were cultured for 12 days until maturation, using orbital shakers, in which they slowly aggregated and formed spheroids in the incubator (at 18 °C). DMEM enabled the formation of spheroids with sufficient size, with an absence of significant necrotic centres, which usually occur in spheroids due to hypoxia. From day 12 to 18, liver spheroids were exposed to single or mixtures of endocrine disruptors (17α-ethinylestradiol and the progestins levonorgestrel and megestrol acetate) to assess their advert effects on cells. Spheroids were evaluated using light and electron microscopy (routine staining and immunohistochemistry), gene expression (a set of genes related to lipid metabolism, yolk proteins and vitellogenin), and biochemical assays (lactate dehydrogenase and resazurin assays). The spheroids were able to respond to the stimuli.
PB  - Czechoslovak Microscopy Society
T2  - 16th Multinational Congress on Microscopy
T1  - Spheroids: in vitro 3D cell cultures of brown trout liver as a model for ecotoxicology research
EP  - 235
SP  - 235
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6941
ER  - 

@misc{
author = "Raskovic, Bozidar and Madureira, Tania Vieria and Lopes, Celia and Rocha, Eduardo",
year = "2022",
abstract = "In 2018, approximately 10.5 million experimental animals were used in the EU for various testing purposes, and 26% of that number accounted for fish. The scientific community, regulatory agencies, and national and EU policymakers endorse the refinement, reduction, and replacement (3Rs) of assays to reduce the number of sacrificed experimental animals. Despite the guidelines in fish exposure assays being well established (such as OECD and US EPA) and the number of test animals kept as low as feasible, each exposure study still requires at least 105 fish. For this reason, many in vitro methods were developed or improved for use in fish assays, which provided a good platform for testing the effects of chemicals, complementing, or replacing in vivo studies. Methods from mammalian in vitro testing were used to develop primary piscine cultures and cell lines, typically cultured in monolayers, viz. two-dimensional (2D). The advantages of 2D cultures are rapid exposure tests, being easy for handling and requiring low-cost maintenance. However, the uses of 2D cultures also have downsides: cells cannot last for a long time and cannot mimic the organization of tissues since they are cultured in the bottom of flasks and plates. To solve these problems, researchers created three-dimensional (3D) cultures. The microarchitecture and physiology of cells in 3D cultures are closer to in vivo systems. 3D primary cultures in fish have been seldom used, but they may provide an excellent research tool for the toxicological assessment of chemicals. To further use fish spheroids in toxicology, we are expanding a protocol for routine isolation and culture/co-culture of cells from brown trout (Salmo trutta) liver. The protocol is compatible with exploring differential centrifugation of isolated cells to change the composition of spheroids in terms of the ratio of hepatocytes and biliary epithelial cells. After plating in non-adhesive plates, cells were cultured for 12 days until maturation, using orbital shakers, in which they slowly aggregated and formed spheroids in the incubator (at 18 °C). DMEM enabled the formation of spheroids with sufficient size, with an absence of significant necrotic centres, which usually occur in spheroids due to hypoxia. From day 12 to 18, liver spheroids were exposed to single or mixtures of endocrine disruptors (17α-ethinylestradiol and the progestins levonorgestrel and megestrol acetate) to assess their advert effects on cells. Spheroids were evaluated using light and electron microscopy (routine staining and immunohistochemistry), gene expression (a set of genes related to lipid metabolism, yolk proteins and vitellogenin), and biochemical assays (lactate dehydrogenase and resazurin assays). The spheroids were able to respond to the stimuli.",
publisher = "Czechoslovak Microscopy Society",
journal = "16th Multinational Congress on Microscopy",
title = "Spheroids: in vitro 3D cell cultures of brown trout liver as a model for ecotoxicology research",
pages = "235-235",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6941"
}

Raskovic, B., Madureira, T. V., Lopes, C.,& Rocha, E.. (2022). Spheroids: in vitro 3D cell cultures of brown trout liver as a model for ecotoxicology research. in 16th Multinational Congress on Microscopy
Czechoslovak Microscopy Society., 235-235.
https://hdl.handle.net/21.15107/rcub_agrospace_6941

Raskovic B, Madureira TV, Lopes C, Rocha E. Spheroids: in vitro 3D cell cultures of brown trout liver as a model for ecotoxicology research. in 16th Multinational Congress on Microscopy. 2022;:235-235.
https://hdl.handle.net/21.15107/rcub_agrospace_6941 .

Raskovic, Bozidar, Madureira, Tania Vieria, Lopes, Celia, Rocha, Eduardo, "Spheroids: in vitro 3D cell cultures of brown trout liver as a model for ecotoxicology research" in 16th Multinational Congress on Microscopy (2022):235-235,
https://hdl.handle.net/21.15107/rcub_agrospace_6941 .

Berillis, P.,& Raskovic, B.. (2022). Histopathology of Aquatic Animals. in Applied Sciences Journal
MDPI., 1-166.
https://doi.org/10.3390/books978-3-0365-3834-1

Berillis P, Raskovic B. Histopathology of Aquatic Animals. in Applied Sciences Journal. 2022;:1-166.
doi:10.3390/books978-3-0365-3834-1 .

Berillis, Panagiotis, Raskovic, Bozidar, "Histopathology of Aquatic Animals" in Applied Sciences Journal (2022):1-166,
https://doi.org/10.3390/books978-3-0365-3834-1 . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Berillis, Panagiotis
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6032
PB  - MDPI
T2  - Applied Sciences (Switzerland)
T1  - Special Issue on the Histopathology of Aquatic Animals
IS  - 3
SP  - 971
VL  - 12
DO  - 10.3390/app12030971
ER  - 

@article{
author = "Rašković, Božidar and Berillis, Panagiotis",
year = "2022",
publisher = "MDPI",
journal = "Applied Sciences (Switzerland)",
title = "Special Issue on the Histopathology of Aquatic Animals",
number = "3",
pages = "971",
volume = "12",
doi = "10.3390/app12030971"
}

Rašković, B.,& Berillis, P.. (2022). Special Issue on the Histopathology of Aquatic Animals. in Applied Sciences (Switzerland)
MDPI., 12(3), 971.
https://doi.org/10.3390/app12030971

Rašković B, Berillis P. Special Issue on the Histopathology of Aquatic Animals. in Applied Sciences (Switzerland). 2022;12(3):971.
doi:10.3390/app12030971 .

Rašković, Božidar, Berillis, Panagiotis, "Special Issue on the Histopathology of Aquatic Animals" in Applied Sciences (Switzerland), 12, no. 3 (2022):971,
https://doi.org/10.3390/app12030971 . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Poleksić, Vesna
AU  - Rašković, Božidar
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5859
AB  - Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garaši reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (IGI) and HP index of gills (IG), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garaši reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Science and Pollution Research
T1  - Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment
DO  - 10.1007/s11356-021-14526-w
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Poleksić, Vesna and Rašković, Božidar",
year = "2021",
abstract = "Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garaši reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (IGI) and HP index of gills (IG), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garaši reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Science and Pollution Research",
title = "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment",
doi = "10.1007/s11356-021-14526-w"
}

Nikolić, D., Skorić, S., Poleksić, V.,& Rašković, B.. (2021). Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s11356-021-14526-w

Nikolić D, Skorić S, Poleksić V, Rašković B. Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research. 2021;.
doi:10.1007/s11356-021-14526-w .

Nikolić, Dušan, Skorić, Stefan, Poleksić, Vesna, Rašković, Božidar, "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment" in Environmental Science and Pollution Research (2021),
https://doi.org/10.1007/s11356-021-14526-w . .

TY  - JOUR
AU  - Kostić‑Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun‑Kolarević, Margareta
AU  - Višnjić‑Jeftić, Željka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković‑Gačić, Branka
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5903
AB  - This study was conducted on the Danube River locality Višnjica, exposed to the discharge of the largest wastewater collector in Serbia’s capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
SP  - 465
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić‑Vuković, Jovana and Kolarević, Stoimir and Kračun‑Kolarević, Margareta and Višnjić‑Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vuković‑Gačić, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Višnjica, exposed to the discharge of the largest wastewater collector in Serbia’s capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
pages = "465",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić‑Vuković, J., Kolarević, S., Kračun‑Kolarević, M., Višnjić‑Jeftić, Ž., Rašković, B., Poleksić, V., Gačić, Z., Lenhardt, M.,& Vuković‑Gačić, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer Science and Business Media Deutschland GmbH., 193(8), 465.
https://doi.org/10.1007/s10661-021-09232-6

Kostić‑Vuković J, Kolarević S, Kračun‑Kolarević M, Višnjić‑Jeftić Ž, Rašković B, Poleksić V, Gačić Z, Lenhardt M, Vuković‑Gačić B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8):465.
doi:10.1007/s10661-021-09232-6 .

Kostić‑Vuković, Jovana, Kolarević, Stoimir, Kračun‑Kolarević, Margareta, Višnjić‑Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vuković‑Gačić, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021):465,
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - JOUR
AU  - Božić, Gavrilo
AU  - Rašković, Božidar
AU  - Stanković, Marko
AU  - Poleksić, Vesna
AU  - Marković, Zoran
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5933
AB  - In the last 60 years, pond farming of common carp (Cyprinus carpio) in Europe has gradually intensified, which has increased the stocking density and amount of supplementary feed, resulting in higher yields. Additionally, compound feed has been introduced to semi-intensive farming to increase the growth of farmed fish. Feed quality affects growth directly through conversion efficiency and indirectly by influencing fish physiology. In the present study, juvenile common carp were grown in net cages in a fishpond for 80 days and fed the same amount of different supplementary feed: wheat, pelleted, or extruded feeds or fish relied only on the available natural food (control group). Growth, microanatomy of the liver and distal intestine, and morphology of erythrocytes were evaluated. Compound feed had beneficial effects on the growth and histological parameters compared to the control group and fish fed wheat. This included higher surface areas of erythrocytes and hepatocytes` nuclei, longer intestinal folds, and thicker tunica muscularis in the distal intestine. The study showed that increased growth and use of compound feeds did not cause any physiological trade-offs that would be depicted in quantitative histomorphometry, and there was an absence of histopathological changes.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Biologia
T1  - Effects of different feeds on growth performance parameters, histology of liver, distal intestine, and erythrocytes morphology of common carp (Cyprinus carpio L.)
DO  - 10.1007/s11756-021-00882-y
ER  - 

@article{
author = "Božić, Gavrilo and Rašković, Božidar and Stanković, Marko and Poleksić, Vesna and Marković, Zoran",
year = "2021",
abstract = "In the last 60 years, pond farming of common carp (Cyprinus carpio) in Europe has gradually intensified, which has increased the stocking density and amount of supplementary feed, resulting in higher yields. Additionally, compound feed has been introduced to semi-intensive farming to increase the growth of farmed fish. Feed quality affects growth directly through conversion efficiency and indirectly by influencing fish physiology. In the present study, juvenile common carp were grown in net cages in a fishpond for 80 days and fed the same amount of different supplementary feed: wheat, pelleted, or extruded feeds or fish relied only on the available natural food (control group). Growth, microanatomy of the liver and distal intestine, and morphology of erythrocytes were evaluated. Compound feed had beneficial effects on the growth and histological parameters compared to the control group and fish fed wheat. This included higher surface areas of erythrocytes and hepatocytes` nuclei, longer intestinal folds, and thicker tunica muscularis in the distal intestine. The study showed that increased growth and use of compound feeds did not cause any physiological trade-offs that would be depicted in quantitative histomorphometry, and there was an absence of histopathological changes.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Biologia",
title = "Effects of different feeds on growth performance parameters, histology of liver, distal intestine, and erythrocytes morphology of common carp (Cyprinus carpio L.)",
doi = "10.1007/s11756-021-00882-y"
}

Božić, G., Rašković, B., Stanković, M., Poleksić, V.,& Marković, Z.. (2021). Effects of different feeds on growth performance parameters, histology of liver, distal intestine, and erythrocytes morphology of common carp (Cyprinus carpio L.). in Biologia
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s11756-021-00882-y

Božić G, Rašković B, Stanković M, Poleksić V, Marković Z. Effects of different feeds on growth performance parameters, histology of liver, distal intestine, and erythrocytes morphology of common carp (Cyprinus carpio L.). in Biologia. 2021;.
doi:10.1007/s11756-021-00882-y .

Božić, Gavrilo, Rašković, Božidar, Stanković, Marko, Poleksić, Vesna, Marković, Zoran, "Effects of different feeds on growth performance parameters, histology of liver, distal intestine, and erythrocytes morphology of common carp (Cyprinus carpio L.)" in Biologia (2021),
https://doi.org/10.1007/s11756-021-00882-y . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Dvořák, Petr
AU  - Mráz, Jan
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5813
AB  - Even though aquaponics is the production system that is increasingly gaining focus, there is an absence of publications studying the use of pesticides in the aquaponics. Therefore, the aim of this study was to assess negative effects of one synthetic (chlorpyrifos) and two botanical insecticides (azadirachtin and pyrethrin) to matured biofilter in plastic buckets. The effectiveness of biofilter bacteria is determined indirectly, as concentrations of ammonia, nitrites and nitrates in the water were measured at 0h, 5h, 13h, 21h, 29h, 37h, 45h and 53h after insecticides application. The results showed negative effects of azadirachtin on the first step of nitrification, as concentration of ammonia was higher comparing to other groups for almost every sample point. Negative effects on second step of nitrification and higher concentrations of nitrites in the water were also detected, but no statistical differences were observed in the present study, due to the large variation between buckets. Nitrates were lower in water treated with azadirachtin from 29 hours from start to the end of the experiment. Application of other two insecticides to biofilter did not cause any effects and showed no difference comparing to the control group.
PB  - Central Fisheries Research Inst
T2  - Turkish Journal of Fisheries and Aquatic Sciences
T1  - Effects of Biodegradable Insecticides on Biofilter Bacteria: Implications for Aquaponics
EP  - 177
IS  - 4
SP  - 169
VL  - 21
DO  - 10.4194/1303-2712-v21_4_02
ER  - 

@article{
author = "Rašković, Božidar and Dvořák, Petr and Mráz, Jan",
year = "2021",
abstract = "Even though aquaponics is the production system that is increasingly gaining focus, there is an absence of publications studying the use of pesticides in the aquaponics. Therefore, the aim of this study was to assess negative effects of one synthetic (chlorpyrifos) and two botanical insecticides (azadirachtin and pyrethrin) to matured biofilter in plastic buckets. The effectiveness of biofilter bacteria is determined indirectly, as concentrations of ammonia, nitrites and nitrates in the water were measured at 0h, 5h, 13h, 21h, 29h, 37h, 45h and 53h after insecticides application. The results showed negative effects of azadirachtin on the first step of nitrification, as concentration of ammonia was higher comparing to other groups for almost every sample point. Negative effects on second step of nitrification and higher concentrations of nitrites in the water were also detected, but no statistical differences were observed in the present study, due to the large variation between buckets. Nitrates were lower in water treated with azadirachtin from 29 hours from start to the end of the experiment. Application of other two insecticides to biofilter did not cause any effects and showed no difference comparing to the control group.",
publisher = "Central Fisheries Research Inst",
journal = "Turkish Journal of Fisheries and Aquatic Sciences",
title = "Effects of Biodegradable Insecticides on Biofilter Bacteria: Implications for Aquaponics",
pages = "177-169",
number = "4",
volume = "21",
doi = "10.4194/1303-2712-v21_4_02"
}

Rašković, B., Dvořák, P.,& Mráz, J.. (2021). Effects of Biodegradable Insecticides on Biofilter Bacteria: Implications for Aquaponics. in Turkish Journal of Fisheries and Aquatic Sciences
Central Fisheries Research Inst., 21(4), 169-177.
https://doi.org/10.4194/1303-2712-v21_4_02

Rašković B, Dvořák P, Mráz J. Effects of Biodegradable Insecticides on Biofilter Bacteria: Implications for Aquaponics. in Turkish Journal of Fisheries and Aquatic Sciences. 2021;21(4):169-177.
doi:10.4194/1303-2712-v21_4_02 .

Rašković, Božidar, Dvořák, Petr, Mráz, Jan, "Effects of Biodegradable Insecticides on Biofilter Bacteria: Implications for Aquaponics" in Turkish Journal of Fisheries and Aquatic Sciences, 21, no. 4 (2021):169-177,
https://doi.org/10.4194/1303-2712-v21_4_02 . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Rašković, Božidar
AU  - Lenhardt, Mirjana
AU  - Krpo-Cetković, Jasmina
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5326
AB  - This study was conducted on individuals of European perch from six reservoirs in Serbia with different properties, used for different purposes, and situated at different altitudes. Samples of muscle, gills, and liver, as well as water samples, were used for analysis of elemental accumulation. Histopathological (HP) analysis of gills and liver, and Fulton's condition factor (CF) were used as biomarkers. Analysis of Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was conducted by inductively-coupled plasma optical emission spectrometry (ICP-OES), and accordingly, metal pollution index and bioaccumulation factor (BAF) were calculated. Elemental accumulation varied considerably both among tissues and reservoirs. Reservoirs located on large low-altitude rivers were the most heavily polluted with metals (Meduvrgje, on the Zapadna Morava River, and Lake Sava, on the Sava River). Of three tissues analyzed, the gills were most affected by pollution. The levels of Hg in muscle decreased with an increase in reservoir altitude. Specimens sampled from the highest altitude reservoir were the least affected by HP alterations; however, HP differences between the reservoirs were not statistically significant, and the severity of HP changes was generally low. No significant correlations between the elemental accumulation or HP indices with CF were observed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)
VL  - 244
DO  - 10.1016/j.chemosphere.2019.125503
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Rašković, Božidar and Lenhardt, Mirjana and Krpo-Cetković, Jasmina",
year = "2020",
abstract = "This study was conducted on individuals of European perch from six reservoirs in Serbia with different properties, used for different purposes, and situated at different altitudes. Samples of muscle, gills, and liver, as well as water samples, were used for analysis of elemental accumulation. Histopathological (HP) analysis of gills and liver, and Fulton's condition factor (CF) were used as biomarkers. Analysis of Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was conducted by inductively-coupled plasma optical emission spectrometry (ICP-OES), and accordingly, metal pollution index and bioaccumulation factor (BAF) were calculated. Elemental accumulation varied considerably both among tissues and reservoirs. Reservoirs located on large low-altitude rivers were the most heavily polluted with metals (Meduvrgje, on the Zapadna Morava River, and Lake Sava, on the Sava River). Of three tissues analyzed, the gills were most affected by pollution. The levels of Hg in muscle decreased with an increase in reservoir altitude. Specimens sampled from the highest altitude reservoir were the least affected by HP alterations; however, HP differences between the reservoirs were not statistically significant, and the severity of HP changes was generally low. No significant correlations between the elemental accumulation or HP indices with CF were observed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)",
volume = "244",
doi = "10.1016/j.chemosphere.2019.125503"
}

Nikolić, D., Skorić, S., Rašković, B., Lenhardt, M.,& Krpo-Cetković, J.. (2020). Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis). in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 244.
https://doi.org/10.1016/j.chemosphere.2019.125503

Nikolić D, Skorić S, Rašković B, Lenhardt M, Krpo-Cetković J. Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis). in Chemosphere. 2020;244.
doi:10.1016/j.chemosphere.2019.125503 .

Nikolić, Dušan, Skorić, Stefan, Rašković, Božidar, Lenhardt, Mirjana, Krpo-Cetković, Jasmina, "Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)" in Chemosphere, 244 (2020),
https://doi.org/10.1016/j.chemosphere.2019.125503 . .

TY  - JOUR
AU  - Imentai, Aiman
AU  - Rašković, Božidar
AU  - Steinbach, Christoph
AU  - Rahimnejad, Samad
AU  - Yanes-Roca, Carlos
AU  - Policar, Tomas
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5307
AB  - This study evaluated the effects of first feeding regime on growth performance, survival rate, and development of digestive organs (intestine, liver and pancreas) in pikeperch (Sander lucioperca) larvae. The fish larvae at 5 days post-hatch (DPH), were initially fed with rotifers (Brachionus plicatilis) for 3 days and from 8 to 17 DPH were fed with rotifers/Artemia for different time periods as follows: (A) only rotifers; (B) 8-13 DPH rotifers/14-17 DPH Artemia; (C) 8-10 DPH rotifers/11-17 DPH Artemia; (D) only Artemia; (E) a combination of rotifers and Artemia. Growth performance, survival rate and histological features of intestine, liver and pancreas were assessed at 11, 14 and 17 DPH to examine the effects of feeding regime. The groups fed rotifers for initial 3 days followed by feeding on Artemia (group D) (53 +/- 5.43%) and combination of rotifers and Artemia (group E) (68 +/- 5.51%), respectively, for the following 9 days showed significantly (P  lt  .05) higher survival rates than the other groups (36-50%). The group fed merely on rotifers (groups A) exhibited significantly lower specific growth rate (SGR) than the other groups, and the highest SGR was found in the group fed with combination of rotifers and Artemia after 3 day rotifer feeding. Moreover, the highest total length (8.57 +/- 0.57 mm), myotome height (0.75 +/- 0.09 mm) and eye diameter (0.58 +/- 0.05 mm) were obtained by combined feeding of rotifers and Artemia after 3 day of initial rotifer feeding. Significant differences among groups were found in morphometric parameters in the anterior intestine and liver. The results of histological examination of the liver, intestine and pancreas did not show any obvious pathological changes in all groups. In conclusion, feeding with rotifers from 5 to 8 DPH and afterwards with Artemia could be suggested as an economical feeding regime for first feeding of pikeperch larvae as comparable survival and growth to co-feeding with rotifers and Artemia were achieved.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - Effects of first feeding regime on growth performance, survival rate and development of digestive system in pikeperch (Sander lucioperca) larvae
VL  - 529
DO  - 10.1016/j.aquaculture.2020.735636
ER  - 

@article{
author = "Imentai, Aiman and Rašković, Božidar and Steinbach, Christoph and Rahimnejad, Samad and Yanes-Roca, Carlos and Policar, Tomas",
year = "2020",
abstract = "This study evaluated the effects of first feeding regime on growth performance, survival rate, and development of digestive organs (intestine, liver and pancreas) in pikeperch (Sander lucioperca) larvae. The fish larvae at 5 days post-hatch (DPH), were initially fed with rotifers (Brachionus plicatilis) for 3 days and from 8 to 17 DPH were fed with rotifers/Artemia for different time periods as follows: (A) only rotifers; (B) 8-13 DPH rotifers/14-17 DPH Artemia; (C) 8-10 DPH rotifers/11-17 DPH Artemia; (D) only Artemia; (E) a combination of rotifers and Artemia. Growth performance, survival rate and histological features of intestine, liver and pancreas were assessed at 11, 14 and 17 DPH to examine the effects of feeding regime. The groups fed rotifers for initial 3 days followed by feeding on Artemia (group D) (53 +/- 5.43%) and combination of rotifers and Artemia (group E) (68 +/- 5.51%), respectively, for the following 9 days showed significantly (P  lt  .05) higher survival rates than the other groups (36-50%). The group fed merely on rotifers (groups A) exhibited significantly lower specific growth rate (SGR) than the other groups, and the highest SGR was found in the group fed with combination of rotifers and Artemia after 3 day rotifer feeding. Moreover, the highest total length (8.57 +/- 0.57 mm), myotome height (0.75 +/- 0.09 mm) and eye diameter (0.58 +/- 0.05 mm) were obtained by combined feeding of rotifers and Artemia after 3 day of initial rotifer feeding. Significant differences among groups were found in morphometric parameters in the anterior intestine and liver. The results of histological examination of the liver, intestine and pancreas did not show any obvious pathological changes in all groups. In conclusion, feeding with rotifers from 5 to 8 DPH and afterwards with Artemia could be suggested as an economical feeding regime for first feeding of pikeperch larvae as comparable survival and growth to co-feeding with rotifers and Artemia were achieved.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "Effects of first feeding regime on growth performance, survival rate and development of digestive system in pikeperch (Sander lucioperca) larvae",
volume = "529",
doi = "10.1016/j.aquaculture.2020.735636"
}

Imentai, A., Rašković, B., Steinbach, C., Rahimnejad, S., Yanes-Roca, C.,& Policar, T.. (2020). Effects of first feeding regime on growth performance, survival rate and development of digestive system in pikeperch (Sander lucioperca) larvae. in Aquaculture
Elsevier, Amsterdam., 529.
https://doi.org/10.1016/j.aquaculture.2020.735636

Imentai A, Rašković B, Steinbach C, Rahimnejad S, Yanes-Roca C, Policar T. Effects of first feeding regime on growth performance, survival rate and development of digestive system in pikeperch (Sander lucioperca) larvae. in Aquaculture. 2020;529.
doi:10.1016/j.aquaculture.2020.735636 .

Imentai, Aiman, Rašković, Božidar, Steinbach, Christoph, Rahimnejad, Samad, Yanes-Roca, Carlos, Policar, Tomas, "Effects of first feeding regime on growth performance, survival rate and development of digestive system in pikeperch (Sander lucioperca) larvae" in Aquaculture, 529 (2020),
https://doi.org/10.1016/j.aquaculture.2020.735636 . .

TY  - JOUR
AU  - Delahaut, Vyshal
AU  - Rašković, Božidar
AU  - Salvado, Marta Satorres
AU  - Bervoets, Lieven
AU  - Blust, Ronny
AU  - De Boeck, Gudrun
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5377
AB  - The individual toxicity and bioaccumulation of cadmium, copper and zinc for common carp juveniles was evaluated in a direct comparison in two experimental setups. First, fish were exposed for 10 days to different metal concentrations in order to link metal bioaccumulation to LC50 values (concentration lethal to 50% of the animals) and incipient lethal levels (ILL, concentration where 50% survives indefinitely). Accumulated metals showed a positive dose dependent uptake for cadmium and copper, but not for zinc. Toxicity was in the order cadmium>copper>zinc with 96h LC50 values for cadmium at 0.20 +/- 0.16 mu M, for copper at 0.77 +/- 0.03 mu M, and for zinc at 29.89 +/- 9.03 mu M respectively. For copper, the 96h exposure was sufficient to calculate the incipient lethal level and therefore 96h LC50 and ILL levels were the same, while for cadmium and zinc 5 to 6 days were needed to reach ILL resulting in slightly lower values at 0.16 mu M and 28.33 mu M respectively. Subsequently, a subacute exposure experiment was conducted, where carp juveniles were exposed to 2 equitoxic concentrations (10% and 50% of LC50 96 h) of the three metals for 1, 3 and 7 days. Again a significant dose-dependent increase in gill cadmium and copper, but not in zinc, was observed during the 7-day exposure. Copper clearly affected sodium levels in gill tissue, while zinc and cadmium did not significantly alter any of the gill electrolytes. The overall histopathological effects (e.g. hyperemia and hypertrophy) of the metal exposures were mild for most of the alterations. Our study showed that copper an cadmium (but not zinc) showed dose dependent metal accumulation, however this bioaccumulation was only correlated with mortality for cadmium. Metal specific alterations were reduced gill sodium levels in copper exposed fish and oedema of the primary epithelium which typically occurred in both levels of zinc exposure.
PB  - Public Library Science, San Francisco
T2  - Plos One
T1  - Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles
IS  - 4
VL  - 15
DO  - 10.1371/journal.pone.0220485
ER  - 

@article{
author = "Delahaut, Vyshal and Rašković, Božidar and Salvado, Marta Satorres and Bervoets, Lieven and Blust, Ronny and De Boeck, Gudrun",
year = "2020",
abstract = "The individual toxicity and bioaccumulation of cadmium, copper and zinc for common carp juveniles was evaluated in a direct comparison in two experimental setups. First, fish were exposed for 10 days to different metal concentrations in order to link metal bioaccumulation to LC50 values (concentration lethal to 50% of the animals) and incipient lethal levels (ILL, concentration where 50% survives indefinitely). Accumulated metals showed a positive dose dependent uptake for cadmium and copper, but not for zinc. Toxicity was in the order cadmium>copper>zinc with 96h LC50 values for cadmium at 0.20 +/- 0.16 mu M, for copper at 0.77 +/- 0.03 mu M, and for zinc at 29.89 +/- 9.03 mu M respectively. For copper, the 96h exposure was sufficient to calculate the incipient lethal level and therefore 96h LC50 and ILL levels were the same, while for cadmium and zinc 5 to 6 days were needed to reach ILL resulting in slightly lower values at 0.16 mu M and 28.33 mu M respectively. Subsequently, a subacute exposure experiment was conducted, where carp juveniles were exposed to 2 equitoxic concentrations (10% and 50% of LC50 96 h) of the three metals for 1, 3 and 7 days. Again a significant dose-dependent increase in gill cadmium and copper, but not in zinc, was observed during the 7-day exposure. Copper clearly affected sodium levels in gill tissue, while zinc and cadmium did not significantly alter any of the gill electrolytes. The overall histopathological effects (e.g. hyperemia and hypertrophy) of the metal exposures were mild for most of the alterations. Our study showed that copper an cadmium (but not zinc) showed dose dependent metal accumulation, however this bioaccumulation was only correlated with mortality for cadmium. Metal specific alterations were reduced gill sodium levels in copper exposed fish and oedema of the primary epithelium which typically occurred in both levels of zinc exposure.",
publisher = "Public Library Science, San Francisco",
journal = "Plos One",
title = "Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles",
number = "4",
volume = "15",
doi = "10.1371/journal.pone.0220485"
}

Delahaut, V., Rašković, B., Salvado, M. S., Bervoets, L., Blust, R.,& De Boeck, G.. (2020). Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles. in Plos One
Public Library Science, San Francisco., 15(4).
https://doi.org/10.1371/journal.pone.0220485

Delahaut V, Rašković B, Salvado MS, Bervoets L, Blust R, De Boeck G. Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles. in Plos One. 2020;15(4).
doi:10.1371/journal.pone.0220485 .

Delahaut, Vyshal, Rašković, Božidar, Salvado, Marta Satorres, Bervoets, Lieven, Blust, Ronny, De Boeck, Gudrun, "Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles" in Plos One, 15, no. 4 (2020),
https://doi.org/10.1371/journal.pone.0220485 . .

TY  - JOUR
AU  - Ljubobratović, Uroš
AU  - Rašković, Božidar
AU  - Balogh, Endre
AU  - Lengyel, Svetlana
AU  - Kovacs, Gyula
AU  - Janurik, Endre
AU  - Poleksić, Vesna
AU  - Ronyai, Andras
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4939
AB  - The aim of the present study was to compare the weight, specific growth rate, length, Fulton's condition factor, survival, mortality, cannibalism and gill histopathology (HP) of pikeperch Sander lucioperca (L. 1758) larvae (12days post-hatch larvae; weight 2.8 +/- 0.9mg) that were cultured in either clear (10 NTU) or clay-turbid water (35 NTU) during a 14-day interval, with an additional 7days of rearing in clear water for both groups. Though higher survival was found in turbid water treatment (15.4 +/- 1.0% vs. 9.4 +/- 3.6%), none of the parameters differed significantly between groups, except during the first 4days of the trial when turbid water significantly decreased the mortality of pikeperch larvae. Concerning HP alterations, a difference between groups in this study was confirmed, since hyperplasia of epithelium and total HP index were statistically higher in the group reared in turbid water. The majority of HP alterations increased during the course of the trial in both groups. The findings in this study confirmed some benefits of using kaolin clay in larval rearing of pikeperch but also showed some adverse effects on the morphology of their branchial apparatus.
PB  - Springer, Dordrecht
T2  - Aquaculture International
T1  - Gill histopathological indicators in pikeperch Sander lucioperca larvae reared in a flow-through system: effect of clay-turbid water
EP  - 1091
IS  - 4
SP  - 1079
VL  - 27
DO  - 10.1007/s10499-019-00393-3
ER  - 

@article{
author = "Ljubobratović, Uroš and Rašković, Božidar and Balogh, Endre and Lengyel, Svetlana and Kovacs, Gyula and Janurik, Endre and Poleksić, Vesna and Ronyai, Andras",
year = "2019",
abstract = "The aim of the present study was to compare the weight, specific growth rate, length, Fulton's condition factor, survival, mortality, cannibalism and gill histopathology (HP) of pikeperch Sander lucioperca (L. 1758) larvae (12days post-hatch larvae; weight 2.8 +/- 0.9mg) that were cultured in either clear (10 NTU) or clay-turbid water (35 NTU) during a 14-day interval, with an additional 7days of rearing in clear water for both groups. Though higher survival was found in turbid water treatment (15.4 +/- 1.0% vs. 9.4 +/- 3.6%), none of the parameters differed significantly between groups, except during the first 4days of the trial when turbid water significantly decreased the mortality of pikeperch larvae. Concerning HP alterations, a difference between groups in this study was confirmed, since hyperplasia of epithelium and total HP index were statistically higher in the group reared in turbid water. The majority of HP alterations increased during the course of the trial in both groups. The findings in this study confirmed some benefits of using kaolin clay in larval rearing of pikeperch but also showed some adverse effects on the morphology of their branchial apparatus.",
publisher = "Springer, Dordrecht",
journal = "Aquaculture International",
title = "Gill histopathological indicators in pikeperch Sander lucioperca larvae reared in a flow-through system: effect of clay-turbid water",
pages = "1091-1079",
number = "4",
volume = "27",
doi = "10.1007/s10499-019-00393-3"
}

Ljubobratović, U., Rašković, B., Balogh, E., Lengyel, S., Kovacs, G., Janurik, E., Poleksić, V.,& Ronyai, A.. (2019). Gill histopathological indicators in pikeperch Sander lucioperca larvae reared in a flow-through system: effect of clay-turbid water. in Aquaculture International
Springer, Dordrecht., 27(4), 1079-1091.
https://doi.org/10.1007/s10499-019-00393-3

Ljubobratović U, Rašković B, Balogh E, Lengyel S, Kovacs G, Janurik E, Poleksić V, Ronyai A. Gill histopathological indicators in pikeperch Sander lucioperca larvae reared in a flow-through system: effect of clay-turbid water. in Aquaculture International. 2019;27(4):1079-1091.
doi:10.1007/s10499-019-00393-3 .

Ljubobratović, Uroš, Rašković, Božidar, Balogh, Endre, Lengyel, Svetlana, Kovacs, Gyula, Janurik, Endre, Poleksić, Vesna, Ronyai, Andras, "Gill histopathological indicators in pikeperch Sander lucioperca larvae reared in a flow-through system: effect of clay-turbid water" in Aquaculture International, 27, no. 4 (2019):1079-1091,
https://doi.org/10.1007/s10499-019-00393-3 . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Cruzeiro, Catarina
AU  - Poleksić, Vesna
AU  - Rocha, Eduardo
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5117
AB  - Assessing fish liver status is common in aquaculture nutrition assays. This often implies determining hepatocytes profile areas in routine thin (5-7 mu m) histological sections. However, there are theoretical problems using planar morphometry in thin sections: inherent sampling cells biases, too small numbers of sampled cells, under/overestimation of size, measuring size as areas when cells are three-dimensional (3D) entities. The gold standard for assessing/validate cell size is stereology using thick sections (20-40 mu m). Here, we estimated the volume of hepatocytes and their nuclei by the nucleator and optical disector stereological probes (in thick sections), and, innovatively, in thin sections too (using single-section disectors). The liver of common carp eating feed containing either low or high level of lipids was targeted. Results were compared with prior profile areas from planar morphometry using thin sections, and with profile areas estimated here with the two-dimensional (2D) nucleator. Ratios between nucleus and cell/cytoplasm (N/C) areas and volumes were calculated and compared. There was high positive correlation between volumes in thin and thick sections (r = .85 to .89; p  lt  .001), empirically validating the single-section disector. Strong correlations existed between profile-derived versus 2D-nucleator areas (r = .74 to .83; p  lt  .001). There was systematic underestimation of cells and nucleus size using planar morphometry. The N/C ratios derived from the 2D-nucleator data were higher than those from planar morphometry. Despite theoretical premises for using simple planar morphometry in thin sections are flawed, our results support that such morphometry on carp/fish hepatocytes may offer some valid biological conclusions. Anyway, we advanced guidelines for implementing proper methods.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Estimating volumes from common carp hepatocytes using design-based stereology and examining correlations with profile areas: Revisiting a nutritional assay and unveiling guidelines to microscopists
EP  - 871
IS  - 6
SP  - 861
VL  - 82
DO  - 10.1002/jemt.23228
ER  - 

@article{
author = "Rašković, Božidar and Cruzeiro, Catarina and Poleksić, Vesna and Rocha, Eduardo",
year = "2019",
abstract = "Assessing fish liver status is common in aquaculture nutrition assays. This often implies determining hepatocytes profile areas in routine thin (5-7 mu m) histological sections. However, there are theoretical problems using planar morphometry in thin sections: inherent sampling cells biases, too small numbers of sampled cells, under/overestimation of size, measuring size as areas when cells are three-dimensional (3D) entities. The gold standard for assessing/validate cell size is stereology using thick sections (20-40 mu m). Here, we estimated the volume of hepatocytes and their nuclei by the nucleator and optical disector stereological probes (in thick sections), and, innovatively, in thin sections too (using single-section disectors). The liver of common carp eating feed containing either low or high level of lipids was targeted. Results were compared with prior profile areas from planar morphometry using thin sections, and with profile areas estimated here with the two-dimensional (2D) nucleator. Ratios between nucleus and cell/cytoplasm (N/C) areas and volumes were calculated and compared. There was high positive correlation between volumes in thin and thick sections (r = .85 to .89; p  lt  .001), empirically validating the single-section disector. Strong correlations existed between profile-derived versus 2D-nucleator areas (r = .74 to .83; p  lt  .001). There was systematic underestimation of cells and nucleus size using planar morphometry. The N/C ratios derived from the 2D-nucleator data were higher than those from planar morphometry. Despite theoretical premises for using simple planar morphometry in thin sections are flawed, our results support that such morphometry on carp/fish hepatocytes may offer some valid biological conclusions. Anyway, we advanced guidelines for implementing proper methods.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Estimating volumes from common carp hepatocytes using design-based stereology and examining correlations with profile areas: Revisiting a nutritional assay and unveiling guidelines to microscopists",
pages = "871-861",
number = "6",
volume = "82",
doi = "10.1002/jemt.23228"
}

Rašković, B., Cruzeiro, C., Poleksić, V.,& Rocha, E.. (2019). Estimating volumes from common carp hepatocytes using design-based stereology and examining correlations with profile areas: Revisiting a nutritional assay and unveiling guidelines to microscopists. in Microscopy Research and Technique
Wiley, Hoboken., 82(6), 861-871.
https://doi.org/10.1002/jemt.23228

Rašković B, Cruzeiro C, Poleksić V, Rocha E. Estimating volumes from common carp hepatocytes using design-based stereology and examining correlations with profile areas: Revisiting a nutritional assay and unveiling guidelines to microscopists. in Microscopy Research and Technique. 2019;82(6):861-871.
doi:10.1002/jemt.23228 .

Rašković, Božidar, Cruzeiro, Catarina, Poleksić, Vesna, Rocha, Eduardo, "Estimating volumes from common carp hepatocytes using design-based stereology and examining correlations with profile areas: Revisiting a nutritional assay and unveiling guidelines to microscopists" in Microscopy Research and Technique, 82, no. 6 (2019):861-871,
https://doi.org/10.1002/jemt.23228 . .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Bosko
AU  - Rašković, Božidar
AU  - Jovanović, Vladimir
AU  - Lazarević, Milos
AU  - Milošević, Maja
AU  - Nikolić, Nadja
AU  - Panajotović, Radmila
AU  - Milasin, Jelena
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5100
AB  - Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.
PB  - Medicinska Naklada, Zagreb
T2  - Croatian Medical Journal
T1  - Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells
EP  - 86
IS  - 2
SP  - 78
VL  - 60
DO  - 10.3325/CroatMedJ_60_0078
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Bosko and Rašković, Božidar and Jovanović, Vladimir and Lazarević, Milos and Milošević, Maja and Nikolić, Nadja and Panajotović, Radmila and Milasin, Jelena",
year = "2019",
abstract = "Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.",
publisher = "Medicinska Naklada, Zagreb",
journal = "Croatian Medical Journal",
title = "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells",
pages = "86-78",
number = "2",
volume = "60",
doi = "10.3325/CroatMedJ_60_0078"
}

Simonović, J., Toljić, B., Rašković, B., Jovanović, V., Lazarević, M., Milošević, M., Nikolić, N., Panajotović, R.,& Milasin, J.. (2019). Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal
Medicinska Naklada, Zagreb., 60(2), 78-86.
https://doi.org/10.3325/CroatMedJ_60_0078

Simonović J, Toljić B, Rašković B, Jovanović V, Lazarević M, Milošević M, Nikolić N, Panajotović R, Milasin J. Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal. 2019;60(2):78-86.
doi:10.3325/CroatMedJ_60_0078 .

Simonović, Jelena, Toljić, Bosko, Rašković, Božidar, Jovanović, Vladimir, Lazarević, Milos, Milošević, Maja, Nikolić, Nadja, Panajotović, Radmila, Milasin, Jelena, "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells" in Croatian Medical Journal, 60, no. 2 (2019):78-86,
https://doi.org/10.3325/CroatMedJ_60_0078 . .

TY  - JOUR
AU  - Molerović, Nikola
AU  - Rašković, Božidar
AU  - Djedović, Radica
AU  - Ostojić-Andrić, Dušica
AU  - Marković, Zoran
AU  - Marić, Saša
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5196
AB  - Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock.
AB  - U cilju zaštite ţivotne sredine i genetiĉkih resursa potoĉne pastrmke (Salmo trutta) u radu je ispitivana genetiĉka struktura ove vrste na ribnjaku ―Braduljica‖ u blizini Ivanjice. DNK je izolovana iz odseĉaka peraja 10 jedinki, a nakon toga molekularne PCR-RFLP metode su korišćene za razlikovanje izmeĊu atlantske i dunavske linije na bazi kontrolnog regiona mitohondrijalne DNK (KR mtDNK) i jedarnog gena za laktat dehidrogenazu (LDH nDNK). Na osnovu fenotipskih karakteristika procenjeno je da je pet jedinki pripadalo alohtonoj atlantskoj liniji, a preostalih pet autohtonoj dunavskoj liniji potoĉne pastrmke. MeĊutim, rezultati molekularnih analiza pokazali su prisustvo visokog procenta alohtonih gena u analiziranom uzorku, što potvrĊuje hibridizaciju izmeĊu ove dve linije. TakoĊe, rezultati ukazuju na to da selekcija na osnovu fenotipa nije adekvatna. Kako bi ribnjak nastavio da se bavi gajenjem potoĉne pastrmke za potrebe poribljavanja okolnih reka, neophodno je da se eliminišu jedinke atlantske linije iz matiĉnog fonda.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia
T1  - Određivanje genetičke strukture potočne pastrmke (Salmo trutta) iz ribnjaka "Braduljica", Srbija
EP  - 299
IS  - 3
SP  - 289
VL  - 35
DO  - 10.2298/BAH1903289M
ER  - 

@article{
author = "Molerović, Nikola and Rašković, Božidar and Djedović, Radica and Ostojić-Andrić, Dušica and Marković, Zoran and Marić, Saša",
year = "2019",
abstract = "Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock., U cilju zaštite ţivotne sredine i genetiĉkih resursa potoĉne pastrmke (Salmo trutta) u radu je ispitivana genetiĉka struktura ove vrste na ribnjaku ―Braduljica‖ u blizini Ivanjice. DNK je izolovana iz odseĉaka peraja 10 jedinki, a nakon toga molekularne PCR-RFLP metode su korišćene za razlikovanje izmeĊu atlantske i dunavske linije na bazi kontrolnog regiona mitohondrijalne DNK (KR mtDNK) i jedarnog gena za laktat dehidrogenazu (LDH nDNK). Na osnovu fenotipskih karakteristika procenjeno je da je pet jedinki pripadalo alohtonoj atlantskoj liniji, a preostalih pet autohtonoj dunavskoj liniji potoĉne pastrmke. MeĊutim, rezultati molekularnih analiza pokazali su prisustvo visokog procenta alohtonih gena u analiziranom uzorku, što potvrĊuje hibridizaciju izmeĊu ove dve linije. TakoĊe, rezultati ukazuju na to da selekcija na osnovu fenotipa nije adekvatna. Kako bi ribnjak nastavio da se bavi gajenjem potoĉne pastrmke za potrebe poribljavanja okolnih reka, neophodno je da se eliminišu jedinke atlantske linije iz matiĉnog fonda.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia, Određivanje genetičke strukture potočne pastrmke (Salmo trutta) iz ribnjaka "Braduljica", Srbija",
pages = "299-289",
number = "3",
volume = "35",
doi = "10.2298/BAH1903289M"
}

Molerović, N., Rašković, B., Djedović, R., Ostojić-Andrić, D., Marković, Z.,& Marić, S.. (2019). Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(3), 289-299.
https://doi.org/10.2298/BAH1903289M

Molerović N, Rašković B, Djedović R, Ostojić-Andrić D, Marković Z, Marić S. Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia. in Biotechnology in Animal Husbandry. 2019;35(3):289-299.
doi:10.2298/BAH1903289M .

Molerović, Nikola, Rašković, Božidar, Djedović, Radica, Ostojić-Andrić, Dušica, Marković, Zoran, Marić, Saša, "Characterization of the genetic structure of the brown trout (Salmo trutta) from "Braduljica" fish farm, Serbia" in Biotechnology in Animal Husbandry, 35, no. 3 (2019):289-299,
https://doi.org/10.2298/BAH1903289M . .

TY  - CONF
AU  - Marković, Zoran
AU  - Stanković, Marko
AU  - Rašković, Božidar
AU  - Sekulić, Nenad
AU  - Poleksić, Vesna
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4688
AB  - Aquaculture is a type of food production with the highest rate of growth. Each year, compared with the previous, 9% more aquatic organisms are produced. Considering limited fish catch from world seas, increased consumption, as well as human population increase on the planet, it is realistic to expect that in the next period the need for fish from aquaculture will raise from year to year. However aquaculture importance is not only as food supply, but also reproduction and culture (farming) of aquatic organisms in order to protect and preserve endangered species of aquatic organisms in natural water ecosystems. Out of approximately 100 fish species inhabiting waters of the Republic of Serbia, 63 are under certain regime of protection (34 species in the status of "strictly protected" wild fish species and 29 wild species in the status protected"). Reasons for decrease of fish diversity in Serbia are many: often there is influence of various factors, seldom one can be sorted out as dominant. Factors most often influencing one or more fish species can be grouped as those originating from changes in the biotope, water quality deterioration, arrival of alochthonous fish species, spreading of diseases and increased pressure on fish resources from men and piscivorous animals. After elimination of factors endangering fish diversity, processes of natural recovery of the biocenosis, primarily fish fauna, mainly requires a long period. However natural recovery not only lasts too much, but this process can be unstable since fish abundance may vary, sensitivity can increase due to different reproductive characteristics, and different fish species have different diseases resistance. All this make natural recovery vulnerable to the negative influences. In such situation it is advisable to help endangered aquatic ecosystems. Among most simple ways, sometimes unique, is restocking with fish obtained from aquaculture. Aquaculture is of greatest importance for endangered fish species protection, but also for preservation of stability of economically important species that are under pressure higher than their natural reproductive potential. Although there are more than 200 fish farms in Serbia, small number of those is oriented towards reproduction and culture of endangered fish species. There are only two coldwater farms of small capacity continuously producing fry of coldwater species (Salmo trutta; Hucho hucho; Thymallus thymallus). Two hatcheries for warmwater fish are caring out artificial spawning from time to time, several warmwater farms periodically and casually produce fry of a small number of strictly protected and protected wild warmwater fish species (Cyprinus carpio; Esox lucius; Sander lucioperca; Silurus glanis), mainly for restocking of fishing waters. Reasons why a small number of farms are oriented towards reproduction and fry culture of endangered species lies primarily in the fact that there is no continual demand for strictly protected and protected wild fish species for restocking fishery waters, i.e. lack of economical reasons for such continuous activity. Although fish farms cooperate sometimes with experts and research institutions, there is a lack of programs and particularly financial support, therefore results are missing. Fry production is based mainly on a small number of broodstock caught from natural environment. There is also lack of genetic analyses and information on "species purity" and genotypes. The importance of restocking with autochthonous genotypes is not taken into account. So far the management of fishery waters has resulted in poor condition of the fish fund in majority of our natural aquatic ecosystems, therefore there is a need to increase protection of fish biodiversity. In order to ensure a more effective system it is necessary to obtain financing for new program realization. Two types of programs should be envisaged: those oriented towards economically important and those oriented towards economically not important fish species. For programs supposed profitable, financing could be assured from users of fishery waters. They could obtain a right to use parts of fishery waters for a limited period. Programs for economically not important species should be by financed by the state. The State will compensate from taxes collected from companies that endanger biodiversity with their wastewater. Programs for economically important autochthonous endangered fish species should be realized based on experiences from many western European countries that are attractive destinations for fishery tourism. Such programs are connecting ecological and economic interests. Economic interests are the basis of the decrease or elimination of migrations from small rural areas to big cities. Fishery tourism is a stimulus to economic development, but also to sustainability of aquatic ecosystems, since these ecosystems are in the focus of development. All this contribute to the tourism of the country, as well. Establishment of profitable programs in Serbia could be based on both autochthonous coldwater and warmwater fish. Programs for coldwater fish will be realized on many attractive hilly mountain destinations, where there are rivers: Drina, River Radovanska, Gradac, Grza, Temska, Veliki Rzav, Moravica, Uvac, Djetinja, Lipatnica, Jerma, Vlasina, Rasina etc. Programs based on warmwater fish species will be realized on lowland rivers, primarily on the Danube, where, besides recreational and sport fishery a new touristic product - economic/professional fishery could be introduced. It is almost impossible to realize all the mentioned programs without aquaculture, more precisely, aquaculture should be established on protection of autochthonous genotypes and species of each river basin, based on knowledge, skills, and values; on ecologically responsible protection of endangered fish species; and economically realistic. Firstly, tax should be paid by farms that omitted to build fish passes, and reproduction sites for migratory species should be revitalized. In addition a support should be given for the existing farms, institutions and teams, and help their activities to become sustainable. Than, new centers for reproduction, culture and restocking fish species should be established. In order to set up and maintain such programs it is indispensable to finance research institutions through new project calls in order to solve the problems and gain new technologies for reproduction and breeding primarily species in focus of the programs.
PB  - Serbian Acad Sciences Arts, Belgrade
C3  - Ecological and Economic Significance of Fauna of Serbia
T1  - Aquaculture in service of edangered fish species protection in Serbia
EP  - 197
SP  - 173
VL  - 171
UR  - https://hdl.handle.net/21.15107/rcub_dais_10627
ER  - 

@conference{
author = "Marković, Zoran and Stanković, Marko and Rašković, Božidar and Sekulić, Nenad and Poleksić, Vesna",
year = "2018",
abstract = "Aquaculture is a type of food production with the highest rate of growth. Each year, compared with the previous, 9% more aquatic organisms are produced. Considering limited fish catch from world seas, increased consumption, as well as human population increase on the planet, it is realistic to expect that in the next period the need for fish from aquaculture will raise from year to year. However aquaculture importance is not only as food supply, but also reproduction and culture (farming) of aquatic organisms in order to protect and preserve endangered species of aquatic organisms in natural water ecosystems. Out of approximately 100 fish species inhabiting waters of the Republic of Serbia, 63 are under certain regime of protection (34 species in the status of "strictly protected" wild fish species and 29 wild species in the status protected"). Reasons for decrease of fish diversity in Serbia are many: often there is influence of various factors, seldom one can be sorted out as dominant. Factors most often influencing one or more fish species can be grouped as those originating from changes in the biotope, water quality deterioration, arrival of alochthonous fish species, spreading of diseases and increased pressure on fish resources from men and piscivorous animals. After elimination of factors endangering fish diversity, processes of natural recovery of the biocenosis, primarily fish fauna, mainly requires a long period. However natural recovery not only lasts too much, but this process can be unstable since fish abundance may vary, sensitivity can increase due to different reproductive characteristics, and different fish species have different diseases resistance. All this make natural recovery vulnerable to the negative influences. In such situation it is advisable to help endangered aquatic ecosystems. Among most simple ways, sometimes unique, is restocking with fish obtained from aquaculture. Aquaculture is of greatest importance for endangered fish species protection, but also for preservation of stability of economically important species that are under pressure higher than their natural reproductive potential. Although there are more than 200 fish farms in Serbia, small number of those is oriented towards reproduction and culture of endangered fish species. There are only two coldwater farms of small capacity continuously producing fry of coldwater species (Salmo trutta; Hucho hucho; Thymallus thymallus). Two hatcheries for warmwater fish are caring out artificial spawning from time to time, several warmwater farms periodically and casually produce fry of a small number of strictly protected and protected wild warmwater fish species (Cyprinus carpio; Esox lucius; Sander lucioperca; Silurus glanis), mainly for restocking of fishing waters. Reasons why a small number of farms are oriented towards reproduction and fry culture of endangered species lies primarily in the fact that there is no continual demand for strictly protected and protected wild fish species for restocking fishery waters, i.e. lack of economical reasons for such continuous activity. Although fish farms cooperate sometimes with experts and research institutions, there is a lack of programs and particularly financial support, therefore results are missing. Fry production is based mainly on a small number of broodstock caught from natural environment. There is also lack of genetic analyses and information on "species purity" and genotypes. The importance of restocking with autochthonous genotypes is not taken into account. So far the management of fishery waters has resulted in poor condition of the fish fund in majority of our natural aquatic ecosystems, therefore there is a need to increase protection of fish biodiversity. In order to ensure a more effective system it is necessary to obtain financing for new program realization. Two types of programs should be envisaged: those oriented towards economically important and those oriented towards economically not important fish species. For programs supposed profitable, financing could be assured from users of fishery waters. They could obtain a right to use parts of fishery waters for a limited period. Programs for economically not important species should be by financed by the state. The State will compensate from taxes collected from companies that endanger biodiversity with their wastewater. Programs for economically important autochthonous endangered fish species should be realized based on experiences from many western European countries that are attractive destinations for fishery tourism. Such programs are connecting ecological and economic interests. Economic interests are the basis of the decrease or elimination of migrations from small rural areas to big cities. Fishery tourism is a stimulus to economic development, but also to sustainability of aquatic ecosystems, since these ecosystems are in the focus of development. All this contribute to the tourism of the country, as well. Establishment of profitable programs in Serbia could be based on both autochthonous coldwater and warmwater fish. Programs for coldwater fish will be realized on many attractive hilly mountain destinations, where there are rivers: Drina, River Radovanska, Gradac, Grza, Temska, Veliki Rzav, Moravica, Uvac, Djetinja, Lipatnica, Jerma, Vlasina, Rasina etc. Programs based on warmwater fish species will be realized on lowland rivers, primarily on the Danube, where, besides recreational and sport fishery a new touristic product - economic/professional fishery could be introduced. It is almost impossible to realize all the mentioned programs without aquaculture, more precisely, aquaculture should be established on protection of autochthonous genotypes and species of each river basin, based on knowledge, skills, and values; on ecologically responsible protection of endangered fish species; and economically realistic. Firstly, tax should be paid by farms that omitted to build fish passes, and reproduction sites for migratory species should be revitalized. In addition a support should be given for the existing farms, institutions and teams, and help their activities to become sustainable. Than, new centers for reproduction, culture and restocking fish species should be established. In order to set up and maintain such programs it is indispensable to finance research institutions through new project calls in order to solve the problems and gain new technologies for reproduction and breeding primarily species in focus of the programs.",
publisher = "Serbian Acad Sciences Arts, Belgrade",
journal = "Ecological and Economic Significance of Fauna of Serbia",
title = "Aquaculture in service of edangered fish species protection in Serbia",
pages = "197-173",
volume = "171",
url = "https://hdl.handle.net/21.15107/rcub_dais_10627"
}

Marković, Z., Stanković, M., Rašković, B., Sekulić, N.,& Poleksić, V.. (2018). Aquaculture in service of edangered fish species protection in Serbia. in Ecological and Economic Significance of Fauna of Serbia
Serbian Acad Sciences Arts, Belgrade., 171, 173-197.
https://hdl.handle.net/21.15107/rcub_dais_10627

Marković Z, Stanković M, Rašković B, Sekulić N, Poleksić V. Aquaculture in service of edangered fish species protection in Serbia. in Ecological and Economic Significance of Fauna of Serbia. 2018;171:173-197.
https://hdl.handle.net/21.15107/rcub_dais_10627 .

Marković, Zoran, Stanković, Marko, Rašković, Božidar, Sekulić, Nenad, Poleksić, Vesna, "Aquaculture in service of edangered fish species protection in Serbia" in Ecological and Economic Significance of Fauna of Serbia, 171 (2018):173-197,
https://hdl.handle.net/21.15107/rcub_dais_10627 .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Skorić, Stefan
AU  - Jovicić, Katarina
AU  - Spasić, Sladana
AU  - Hegedis, Aleksandar
AU  - Vasić, Nebojša
AU  - Lenhardt, Mirjana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4745
AB  - Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruscica reservoir (intended for water supply) and Meduvrsje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed. Chub specimens from Korenita River had higher concentrations of As, Ba and Pb in all three investigated tissues as well as higher total histopathological index values than chub from reservoirs. Specimens from Meduvrsje reservoir were characterized by higher values for concentrations of Cu and Si in muscle tissue and higher values for regressive histopathological alterations in gills. Individuals of chub from Kruscica reservoir had the highest concentrations of Fe in liver, Hg in muscle and Sr and Zn in muscle while gills had the lowest value of total histopathological index. The results from the present study showed higher level of histopathological alterations as a result of mine tailing accident. As a result of mixed contamination on the Meduvrsje site, histopathological index values of gills were in line with the index value from Korenita River. Increased values for Fe and Sr in chub tissue from Kruscica reservoir could be explained by geological structure of the site which is characterized by magmatic rock rich in Cu, Fe and Ni as well as dominant carbonate sediment complex of marine origin with increased level of Sr.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Ecotoxicology and Environmental Safety
T1  - Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia
EP  - 247
SP  - 238
VL  - 153
DO  - 10.1016/j.ecoenv.2018.01.058
ER  - 

@article{
author = "Rašković, Božidar and Poleksić, Vesna and Skorić, Stefan and Jovicić, Katarina and Spasić, Sladana and Hegedis, Aleksandar and Vasić, Nebojša and Lenhardt, Mirjana",
year = "2018",
abstract = "Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruscica reservoir (intended for water supply) and Meduvrsje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed. Chub specimens from Korenita River had higher concentrations of As, Ba and Pb in all three investigated tissues as well as higher total histopathological index values than chub from reservoirs. Specimens from Meduvrsje reservoir were characterized by higher values for concentrations of Cu and Si in muscle tissue and higher values for regressive histopathological alterations in gills. Individuals of chub from Kruscica reservoir had the highest concentrations of Fe in liver, Hg in muscle and Sr and Zn in muscle while gills had the lowest value of total histopathological index. The results from the present study showed higher level of histopathological alterations as a result of mine tailing accident. As a result of mixed contamination on the Meduvrsje site, histopathological index values of gills were in line with the index value from Korenita River. Increased values for Fe and Sr in chub tissue from Kruscica reservoir could be explained by geological structure of the site which is characterized by magmatic rock rich in Cu, Fe and Ni as well as dominant carbonate sediment complex of marine origin with increased level of Sr.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Ecotoxicology and Environmental Safety",
title = "Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia",
pages = "247-238",
volume = "153",
doi = "10.1016/j.ecoenv.2018.01.058"
}

Rašković, B., Poleksić, V., Skorić, S., Jovicić, K., Spasić, S., Hegedis, A., Vasić, N.,& Lenhardt, M.. (2018). Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia. in Ecotoxicology and Environmental Safety
Academic Press Inc Elsevier Science, San Diego., 153, 238-247.
https://doi.org/10.1016/j.ecoenv.2018.01.058

Rašković B, Poleksić V, Skorić S, Jovicić K, Spasić S, Hegedis A, Vasić N, Lenhardt M. Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia. in Ecotoxicology and Environmental Safety. 2018;153:238-247.
doi:10.1016/j.ecoenv.2018.01.058 .

Rašković, Božidar, Poleksić, Vesna, Skorić, Stefan, Jovicić, Katarina, Spasić, Sladana, Hegedis, Aleksandar, Vasić, Nebojša, Lenhardt, Mirjana, "Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia" in Ecotoxicology and Environmental Safety, 153 (2018):238-247,
https://doi.org/10.1016/j.ecoenv.2018.01.058 . .

TY  - JOUR
AU  - Shrivastava, Jyotsna
AU  - Rašković, Božidar
AU  - Blust, Ronny
AU  - De Boeck, Gudrun
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4643
AB  - It has been suggested that induced swimming has the potential to improve the growth performance of fish. We tested this hypothesis by measuring growth, metabolic efficiency and physiological capacity of common carp (Cyprinus carpio). Fish were swum at different exercise regimes: 0.0 (control), 1.5 and 2.5 body lengths per second (BL/s) in 1600 L recirculating raceways for 4 weeks. The results showed a significant increase in weight gain, specific growth rate, improved feed conversion efficiency, and a higher hepatosomatic index for 2.5 BL/s exercised fish compared to control. Glycogen, protein and lipid energy stores in hepatic and muscular tissue showed limited differences among experimental groups. Likewise, plasma [Na+], [K+] and [Cl- ] remained stable at all swimming regimes. Expression of genes controlling energy metabolism and growth (IGF-I axis, cytochrome oxidase) and stress response (cortisol receptor, heat shock protein 70) revealed clear regulatory roles as the mRNA transcript levels of IGF-I and growth hormone receptors in hepatic tissue were up-regulated in fish exercised for 3-4 weeks at 2.5 BL/s. Oxygen consumption rate and swimming performance (U-crit) for each experimental group were evaluated in parallel in Blazka-type swim-tunnels (3.9 L) and showed no training effect while prolonged swimming at 1.5 and 2.5 BL/s facilitated ammonia excretion and prevented build-up of plasma ammonia. Overall, these data suggest that sustained exercise at 2.5 BL/s enhanced growth and physiological fitness without compromising energy metabolism or ion-regulation. Our study provides a prospective of implementing exercise as a tool to increase fish production efficiency in commercial aquaculture systems.
PB  - Elsevier Science Inc, New York
T2  - Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
T1  - Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio)
EP  - 48
SP  - 38
VL  - 226
DO  - 10.1016/j.cbpa.2018.08.007
ER  - 

@article{
author = "Shrivastava, Jyotsna and Rašković, Božidar and Blust, Ronny and De Boeck, Gudrun",
year = "2018",
abstract = "It has been suggested that induced swimming has the potential to improve the growth performance of fish. We tested this hypothesis by measuring growth, metabolic efficiency and physiological capacity of common carp (Cyprinus carpio). Fish were swum at different exercise regimes: 0.0 (control), 1.5 and 2.5 body lengths per second (BL/s) in 1600 L recirculating raceways for 4 weeks. The results showed a significant increase in weight gain, specific growth rate, improved feed conversion efficiency, and a higher hepatosomatic index for 2.5 BL/s exercised fish compared to control. Glycogen, protein and lipid energy stores in hepatic and muscular tissue showed limited differences among experimental groups. Likewise, plasma [Na+], [K+] and [Cl- ] remained stable at all swimming regimes. Expression of genes controlling energy metabolism and growth (IGF-I axis, cytochrome oxidase) and stress response (cortisol receptor, heat shock protein 70) revealed clear regulatory roles as the mRNA transcript levels of IGF-I and growth hormone receptors in hepatic tissue were up-regulated in fish exercised for 3-4 weeks at 2.5 BL/s. Oxygen consumption rate and swimming performance (U-crit) for each experimental group were evaluated in parallel in Blazka-type swim-tunnels (3.9 L) and showed no training effect while prolonged swimming at 1.5 and 2.5 BL/s facilitated ammonia excretion and prevented build-up of plasma ammonia. Overall, these data suggest that sustained exercise at 2.5 BL/s enhanced growth and physiological fitness without compromising energy metabolism or ion-regulation. Our study provides a prospective of implementing exercise as a tool to increase fish production efficiency in commercial aquaculture systems.",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology",
title = "Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio)",
pages = "48-38",
volume = "226",
doi = "10.1016/j.cbpa.2018.08.007"
}

Shrivastava, J., Rašković, B., Blust, R.,& De Boeck, G.. (2018). Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio). in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology
Elsevier Science Inc, New York., 226, 38-48.
https://doi.org/10.1016/j.cbpa.2018.08.007

Shrivastava J, Rašković B, Blust R, De Boeck G. Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio). in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology. 2018;226:38-48.
doi:10.1016/j.cbpa.2018.08.007 .

Shrivastava, Jyotsna, Rašković, Božidar, Blust, Ronny, De Boeck, Gudrun, "Exercise improves growth, alters physiological performance and gene expression in common carp (Cyprinus carpio)" in Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 226 (2018):38-48,
https://doi.org/10.1016/j.cbpa.2018.08.007 . .