Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells
2019
Аутори
Simonović, JelenaToljić, Bosko
Rašković, Božidar
Jovanović, Vladimir
Lazarević, Milos
Milošević, Maja
Nikolić, Nadja
Panajotović, Radmila
Milasin, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid p...eaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.
Извор:
Croatian Medical Journal, 2019, 60, 2, 78-86Издавач:
- Medicinska Naklada, Zagreb
Финансирање / пројекти:
- Генетичка контрола и молекуларни механизми у малигним, инфламаторним и развојним патологијама орофацијалне регије (RS-MESTD-Basic Research (BR or ON)-175075)
DOI: 10.3325/CroatMedJ_60_0078
ISSN: 0353-9504
PubMed: 31044579
WoS: 000470656200004
[ Google Scholar ]Институција/група
Poljoprivredni fakultetTY - JOUR AU - Simonović, Jelena AU - Toljić, Bosko AU - Rašković, Božidar AU - Jovanović, Vladimir AU - Lazarević, Milos AU - Milošević, Maja AU - Nikolić, Nadja AU - Panajotović, Radmila AU - Milasin, Jelena PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5100 AB - Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them. PB - Medicinska Naklada, Zagreb T2 - Croatian Medical Journal T1 - Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells EP - 86 IS - 2 SP - 78 VL - 60 DO - 10.3325/CroatMedJ_60_0078 ER -
@article{ author = "Simonović, Jelena and Toljić, Bosko and Rašković, Božidar and Jovanović, Vladimir and Lazarević, Milos and Milošević, Maja and Nikolić, Nadja and Panajotović, Radmila and Milasin, Jelena", year = "2019", abstract = "Aim To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods SCAR DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm(-1) range, and characteristic lipid peaks at positions 1440 cm(-1) and 1650 cm(-1). Conclusion Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them.", publisher = "Medicinska Naklada, Zagreb", journal = "Croatian Medical Journal", title = "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells", pages = "86-78", number = "2", volume = "60", doi = "10.3325/CroatMedJ_60_0078" }
Simonović, J., Toljić, B., Rašković, B., Jovanović, V., Lazarević, M., Milošević, M., Nikolić, N., Panajotović, R.,& Milasin, J.. (2019). Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal Medicinska Naklada, Zagreb., 60(2), 78-86. https://doi.org/10.3325/CroatMedJ_60_0078
Simonović J, Toljić B, Rašković B, Jovanović V, Lazarević M, Milošević M, Nikolić N, Panajotović R, Milasin J. Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. in Croatian Medical Journal. 2019;60(2):78-86. doi:10.3325/CroatMedJ_60_0078 .
Simonović, Jelena, Toljić, Bosko, Rašković, Božidar, Jovanović, Vladimir, Lazarević, Milos, Milošević, Maja, Nikolić, Nadja, Panajotović, Radmila, Milasin, Jelena, "Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells" in Croatian Medical Journal, 60, no. 2 (2019):78-86, https://doi.org/10.3325/CroatMedJ_60_0078 . .