Mathematical modeling of cell distribution in alginate microbeads
Apstrakt
Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.
Ključne reči:
alginate microbeads / cell distribution / cell colonies / cellular automataIzvor:
Materials Science Forum, 2005, 494, 531-536Izdavač:
- 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
DOI: 10.4028/0-87849-971-7.531
ISSN: 0255-5476
WoS: 000230985800087
Scopus: 2-s2.0-35248884720
Institucija/grupa
Poljoprivredni fakultetTY - CONF AU - Obradović, B AU - Bugarski, Branko AU - Todosijević, Z AU - Nedović, Viktor AU - Bugarski, D AU - Vunjak-Novaković, G PY - 2005 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/874 AB - Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time. PB - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes C3 - Materials Science Forum T1 - Mathematical modeling of cell distribution in alginate microbeads EP - 536 SP - 531 VL - 494 DO - 10.4028/0-87849-971-7.531 ER -
@conference{ author = "Obradović, B and Bugarski, Branko and Todosijević, Z and Nedović, Viktor and Bugarski, D and Vunjak-Novaković, G", year = "2005", abstract = "Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.", publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes", journal = "Materials Science Forum", title = "Mathematical modeling of cell distribution in alginate microbeads", pages = "536-531", volume = "494", doi = "10.4028/0-87849-971-7.531" }
Obradović, B., Bugarski, B., Todosijević, Z., Nedović, V., Bugarski, D.,& Vunjak-Novaković, G.. (2005). Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 531-536. https://doi.org/10.4028/0-87849-971-7.531
Obradović B, Bugarski B, Todosijević Z, Nedović V, Bugarski D, Vunjak-Novaković G. Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum. 2005;494:531-536. doi:10.4028/0-87849-971-7.531 .
Obradović, B, Bugarski, Branko, Todosijević, Z, Nedović, Viktor, Bugarski, D, Vunjak-Novaković, G, "Mathematical modeling of cell distribution in alginate microbeads" in Materials Science Forum, 494 (2005):531-536, https://doi.org/10.4028/0-87849-971-7.531 . .