Identification of phytoplasmas associated with grapevine yellows in serbia
Samo za registrovane korisnike
2004
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence doree phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence doree phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characteri...zation of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.
Ključne reči:
grapevine / phytoplasmas / Flavescence doree / Bois noir / European stone fruit yellowsIzvor:
Journal of Phytopathology, 2004, 152, 10, 575-579Izdavač:
- Wiley, Hoboken
DOI: 10.1111/j.1439-0434.2004.00898.x
ISSN: 0931-1785
WoS: 000224524500008
Scopus: 2-s2.0-7644240826
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Duduk, Bojan AU - Botti, S AU - Ivanović, M AU - Krstić, B AU - Dukić, N AU - Bertaccini, Assunta PY - 2004 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/870 AB - The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence doree phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence doree phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characterization of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains. PB - Wiley, Hoboken T2 - Journal of Phytopathology T1 - Identification of phytoplasmas associated with grapevine yellows in serbia EP - 579 IS - 10 SP - 575 VL - 152 DO - 10.1111/j.1439-0434.2004.00898.x ER -
@article{ author = "Duduk, Bojan and Botti, S and Ivanović, M and Krstić, B and Dukić, N and Bertaccini, Assunta", year = "2004", abstract = "The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence doree phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence doree phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characterization of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.", publisher = "Wiley, Hoboken", journal = "Journal of Phytopathology", title = "Identification of phytoplasmas associated with grapevine yellows in serbia", pages = "579-575", number = "10", volume = "152", doi = "10.1111/j.1439-0434.2004.00898.x" }
Duduk, B., Botti, S., Ivanović, M., Krstić, B., Dukić, N.,& Bertaccini, A.. (2004). Identification of phytoplasmas associated with grapevine yellows in serbia. in Journal of Phytopathology Wiley, Hoboken., 152(10), 575-579. https://doi.org/10.1111/j.1439-0434.2004.00898.x
Duduk B, Botti S, Ivanović M, Krstić B, Dukić N, Bertaccini A. Identification of phytoplasmas associated with grapevine yellows in serbia. in Journal of Phytopathology. 2004;152(10):575-579. doi:10.1111/j.1439-0434.2004.00898.x .
Duduk, Bojan, Botti, S, Ivanović, M, Krstić, B, Dukić, N, Bertaccini, Assunta, "Identification of phytoplasmas associated with grapevine yellows in serbia" in Journal of Phytopathology, 152, no. 10 (2004):575-579, https://doi.org/10.1111/j.1439-0434.2004.00898.x . .