The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence doree phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence doree phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characteri...zation of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.
Keywords:
grapevine / phytoplasmas / Flavescence doree / Bois noir / European stone fruit yellows
TY - JOUR
AU - Duduk, Bojan
AU - Botti, S
AU - Ivanović, M
AU - Krstić, B
AU - Dukić, N
AU - Bertaccini, Assunta
PY - 2004
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/870
AB - The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence doree phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence doree phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characterization of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.
PB - Wiley, Hoboken
T2 - Journal of Phytopathology
T1 - Identification of phytoplasmas associated with grapevine yellows in serbia
EP - 579
IS - 10
SP - 575
VL - 152
DO - 10.1111/j.1439-0434.2004.00898.x
ER -
@article{
author = "Duduk, Bojan and Botti, S and Ivanović, M and Krstić, B and Dukić, N and Bertaccini, Assunta",
year = "2004",
abstract = "The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence doree phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Zupa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence doree phytoplasmas from Zupa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characterization of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.",
publisher = "Wiley, Hoboken",
journal = "Journal of Phytopathology",
title = "Identification of phytoplasmas associated with grapevine yellows in serbia",
pages = "579-575",
number = "10",
volume = "152",
doi = "10.1111/j.1439-0434.2004.00898.x"
}
Duduk, B., Botti, S., Ivanović, M., Krstić, B., Dukić, N.,& Bertaccini, A.. (2004). Identification of phytoplasmas associated with grapevine yellows in serbia. in Journal of Phytopathology
Wiley, Hoboken., 152(10), 575-579.
https://doi.org/10.1111/j.1439-0434.2004.00898.x
Duduk B, Botti S, Ivanović M, Krstić B, Dukić N, Bertaccini A. Identification of phytoplasmas associated with grapevine yellows in serbia. in Journal of Phytopathology. 2004;152(10):575-579.
doi:10.1111/j.1439-0434.2004.00898.x .
Duduk, Bojan, Botti, S, Ivanović, M, Krstić, B, Dukić, N, Bertaccini, Assunta, "Identification of phytoplasmas associated with grapevine yellows in serbia" in Journal of Phytopathology, 152, no. 10 (2004):575-579,
https://doi.org/10.1111/j.1439-0434.2004.00898.x . .
