Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia)

Abstract

Screenings conducted in 2002 indicated the occurrence of viral symptoms on pumpkins in the locations of Bački Petrovac, Futog, Žabalj, Titel, Torda and Veliko Selo. Using two methods of viral identification, biotest and DAS−ELISA serological method, we have confirmed the presence of three viruses, CMV, WMV−2 and ZYMV, in pumpkin samples. ZYMV was the predominant virus of pumpkins in 2002. WMV−2 differed significantly in the frequency of occurrence between years. While it occurred sporadically in 2001, it was the second most frequent virus in 2002. CMV was in the second place in 2001 and in the third place in 2002. The observed variability in the occurrence and spread of viruses among the years is frequent in the case of no persistent viruses of pumpkins. It is also an indication of possible expansion of WMV−2 in Serbia.

Ispitivanjima sprovedenim tokom 2002. godine zabeležena je pojava virusnih simptoma na običnoj tikvi u lokalitetima Bački Petrovac, Futog, Žabalj Titel, Torda i Veliko Selo. Primenom dve metode identifikacije virusa biotesta i serološke metode, DAS−ELISA, u uzrocima obične tikve utvrđeno je prisustvo tri virusa CMV, WMV−2 i ZYMV. Praćenjem pojave i rasprostranjenosti virusa na običnoj tikvi tokom 2002. godine, potvrđeno je dominantno prisustvo ZYMV. Značajna razlika u učestalosti pojave, u odnosu na ispitivanja ranijih godina, zabeležena je kod WMV−2, koji se u 2001. javljao sporadično, a u 2002. godini bio drugi po zastupljenosti. CMV je tokom 2001. godine bio drugi po zastupljenosti, a tokom 2002. godine treći. Ova varijabilnost u pojavi i rasprostranjenosti virusa u različitim godinama, česta je pojava kod neperzistentnih virusa na gajenim tikvama, ali govori i o mogućoj ekspanziji WMV−2 u našoj zemlji.