Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12
Само за регистроване кориснике
2023
Аутори
Balanč, BojanaĐorđević, Verica
Salević, Ana
Radovanović, Željko
Nedović, Viktor
Bugarski, Branko
Knežević-Jugović, Zorica
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Leaves available as by-products from some crops could be used as a major protein
source for food applications. Here, pumpkin leaves from field crop side streams were used for
the preparation of a protein-rich extract. Namely, pumpkin leaves were mechanically
processed with additional steps of acidic treatment and lyophilization to produce the extract.
The extract (1 mass%) was mixed with a spinnable biopolymer-pullulan (5 mass%) with the
addition of vitamin B12 (vitB12) as a model vitamin. This homogeneous mixture was
electrospuned through a stainless-steel needle (18G) at a steady flow rate of 25.2 mL/h using
a syringe pump and an electric field (17 kV) between the positively charged needle and
grounded metallic collector plate. The starting solutions (plain pullulan-PUL, pullulan with
extract-PUL+E, and pullulan with extract and vitB12-PUL+E+B12) as well as the resulting
structures were analyzed regarding physicochemical and morphological properties.
The addition of the ex...tract increases the surface tension of the PUL solution, but
vitB12 decreases it. On the other hand, the viscosity measurements showed an increase in the
value with the addition of extract and vitB12. PUL solution had a conductivity of 0.163
mS/cm, and the value increased upon adding the extract and vitB12 (1.420 mS/cm and 0.978
mS/cm, respectively). According to SEM images, electrospinning resulted in the formation of
beaded fibers. The lower the viscosity of the solution, the greater the proportion of particles
observed, which is in accordance with literature data. The mean value of the particle size
decreases from 388.89 nm (PUL) to 176.68 nm (PUL+E). However, the addition of vitB12
leads to an increase in the mean particle size to 255.94 nm. The addition of extract didn't have
a strong effect on average fiber diameter, while a slight increase was observed in
PUL+E+B12. FTIR analysis suggests intermolecular interactions between the constituents of
the pullulan-extract-vitamin B12 fibers. As a conclusion, the combination of pullulan and
protein-rich extract has the properties of a stable carrier for the encapsulation of vitamin B12.
Кључне речи:
electrospinning / pullulan / pumpkin leaves protein / nanostructures / vitamin B12Извор:
Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 2023, 59-Издавач:
- University of Belgrade, Faculty of Technology and Metallurgy
Финансирање / пројекти:
- MultiPromis - Multifunctional leaf protein and assembled nanocarrier structures delivered by enzyme technology (RS-ScienceFundRS-Ideje-7751519)
Институција/група
Poljoprivredni fakultetTY - CONF AU - Balanč, Bojana AU - Đorđević, Verica AU - Salević, Ana AU - Radovanović, Željko AU - Nedović, Viktor AU - Bugarski, Branko AU - Knežević-Jugović, Zorica PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6868 AB - Leaves available as by-products from some crops could be used as a major protein source for food applications. Here, pumpkin leaves from field crop side streams were used for the preparation of a protein-rich extract. Namely, pumpkin leaves were mechanically processed with additional steps of acidic treatment and lyophilization to produce the extract. The extract (1 mass%) was mixed with a spinnable biopolymer-pullulan (5 mass%) with the addition of vitamin B12 (vitB12) as a model vitamin. This homogeneous mixture was electrospuned through a stainless-steel needle (18G) at a steady flow rate of 25.2 mL/h using a syringe pump and an electric field (17 kV) between the positively charged needle and grounded metallic collector plate. The starting solutions (plain pullulan-PUL, pullulan with extract-PUL+E, and pullulan with extract and vitB12-PUL+E+B12) as well as the resulting structures were analyzed regarding physicochemical and morphological properties. The addition of the extract increases the surface tension of the PUL solution, but vitB12 decreases it. On the other hand, the viscosity measurements showed an increase in the value with the addition of extract and vitB12. PUL solution had a conductivity of 0.163 mS/cm, and the value increased upon adding the extract and vitB12 (1.420 mS/cm and 0.978 mS/cm, respectively). According to SEM images, electrospinning resulted in the formation of beaded fibers. The lower the viscosity of the solution, the greater the proportion of particles observed, which is in accordance with literature data. The mean value of the particle size decreases from 388.89 nm (PUL) to 176.68 nm (PUL+E). However, the addition of vitB12 leads to an increase in the mean particle size to 255.94 nm. The addition of extract didn't have a strong effect on average fiber diameter, while a slight increase was observed in PUL+E+B12. FTIR analysis suggests intermolecular interactions between the constituents of the pullulan-extract-vitamin B12 fibers. As a conclusion, the combination of pullulan and protein-rich extract has the properties of a stable carrier for the encapsulation of vitamin B12. PB - University of Belgrade, Faculty of Technology and Metallurgy C3 - Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists T1 - Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12 SP - 59 UR - https://hdl.handle.net/21.15107/rcub_agrospace_6868 ER -
@conference{ author = "Balanč, Bojana and Đorđević, Verica and Salević, Ana and Radovanović, Željko and Nedović, Viktor and Bugarski, Branko and Knežević-Jugović, Zorica", year = "2023", abstract = "Leaves available as by-products from some crops could be used as a major protein source for food applications. Here, pumpkin leaves from field crop side streams were used for the preparation of a protein-rich extract. Namely, pumpkin leaves were mechanically processed with additional steps of acidic treatment and lyophilization to produce the extract. The extract (1 mass%) was mixed with a spinnable biopolymer-pullulan (5 mass%) with the addition of vitamin B12 (vitB12) as a model vitamin. This homogeneous mixture was electrospuned through a stainless-steel needle (18G) at a steady flow rate of 25.2 mL/h using a syringe pump and an electric field (17 kV) between the positively charged needle and grounded metallic collector plate. The starting solutions (plain pullulan-PUL, pullulan with extract-PUL+E, and pullulan with extract and vitB12-PUL+E+B12) as well as the resulting structures were analyzed regarding physicochemical and morphological properties. The addition of the extract increases the surface tension of the PUL solution, but vitB12 decreases it. On the other hand, the viscosity measurements showed an increase in the value with the addition of extract and vitB12. PUL solution had a conductivity of 0.163 mS/cm, and the value increased upon adding the extract and vitB12 (1.420 mS/cm and 0.978 mS/cm, respectively). According to SEM images, electrospinning resulted in the formation of beaded fibers. The lower the viscosity of the solution, the greater the proportion of particles observed, which is in accordance with literature data. The mean value of the particle size decreases from 388.89 nm (PUL) to 176.68 nm (PUL+E). However, the addition of vitB12 leads to an increase in the mean particle size to 255.94 nm. The addition of extract didn't have a strong effect on average fiber diameter, while a slight increase was observed in PUL+E+B12. FTIR analysis suggests intermolecular interactions between the constituents of the pullulan-extract-vitamin B12 fibers. As a conclusion, the combination of pullulan and protein-rich extract has the properties of a stable carrier for the encapsulation of vitamin B12.", publisher = "University of Belgrade, Faculty of Technology and Metallurgy", journal = "Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists", title = "Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12", pages = "59", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6868" }
Balanč, B., Đorđević, V., Salević, A., Radovanović, Ž., Nedović, V., Bugarski, B.,& Knežević-Jugović, Z.. (2023). Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists University of Belgrade, Faculty of Technology and Metallurgy., 59. https://hdl.handle.net/21.15107/rcub_agrospace_6868
Balanč B, Đorđević V, Salević A, Radovanović Ž, Nedović V, Bugarski B, Knežević-Jugović Z. Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists. 2023;:59. https://hdl.handle.net/21.15107/rcub_agrospace_6868 .
Balanč, Bojana, Đorđević, Verica, Salević, Ana, Radovanović, Željko, Nedović, Viktor, Bugarski, Branko, Knežević-Jugović, Zorica, "Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12" in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists (2023):59, https://hdl.handle.net/21.15107/rcub_agrospace_6868 .