Comparative analysis of biofortification effects on phenolic and glucosinolate profiles in Diploptaxis tenuifolia (L.) DC T&T hybrid
Аутори
Kilibarda, SofijaMilinčić, Danijel
Pantović, Jelena
Vuković, Sandra
Moravčević, Đorđe
Pešić, Mirjana
Kostić, Aleksandar Ž.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Wild rocket, scientifically known as Diplotaxis tenuifolia (L.) DC is a leafy green
plant from the Brassicaceae family. Native to the Medditerian region, today this perennial
plant is cultivated worldwide, owing to the distinctive pungent taste of its deeply lobed green
leaves. Beyond their culinary uses, the leaves of wild rocket exhibit potential for
pharmaceutical applications due to their rich bioactive compounds profile. These include
vitamins, pigments (chlorophyll a and b, carotenoids), phenolics, and glucosinolates (GLSs),
all exhibiting strong antioxidant and anti-cancer activities. This study aimed to evaluate the
effects of biofortification, employing biostimulant Kelpak and two distinct foliar fertilizers
(iron and potassium-enriched) in comparison to a control group, on the phenolic and
glucosinolate content in the leaves of wild rocket hybrid T&T F1. Using an ultra-highperformance
liquid chromatography (UHPLC) system, coupled with a quadrupole time-offlight
mas...s spectrometry (Q-ToF-MS), a total of twenty-three plant phenolics was quantified,
and the relative content of four glucosinolates was evaluated in 70% methanol extracts.
Concerning the overall content of phenolic compounds, treatment involving iron application
showed the highest levels at 3549.1 mg/kg of fresh weight (FW), while the control group
exhibited the lowest content (2171.4 mg/kg FW). Moreover, the most prominent phenolics
included quercetin-3,4'-di-O-hexoside-3'-O-(6"-sinapoyl)-hexoside (ranging from 527.2 to
651.8 mg/kg FW among groups) and 1,8-dipropoxyanthraquinone (278.3-558.5 mg/kg FW).
The control and iron-enriched samples exhibited the presence of kaempferol-3,7,4'-tri-Ohexoside
+ HCOOH and isorhamnetin-3-O-hexoside-4'-O-gentobioside, in addition to the
non-identified sinapic acid hydroxide in the Kelpak treated group. Additionally, the
determination of relative GLSs content of glucosativin, glucoerucin, neoglucobrassicin, and
DMB-GLS revealed that glucosativin was the dominant compound, with proportions ranging
from 79.8% (Kelpak treatments) to 89.1% (iron treatments). In conclusion, selected hybrid
biofortification treatments, notably through the use of iron foliar fertilizers, enhanced
phenolic and glucosinolate leaf content, highlighting its potential significance in nutrition and
medicine.
Кључне речи:
glucosinolates / phenolics / UHPLC-Q-ToF-MS / wild rocketИзвор:
International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 2023, 13-13Издавач:
- UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200054 (Истраживачко-развојни институт ТАМИШ) (RS-MESTD-inst-2020-200054)
- FUNPRO - Functional products based on goat's milk proteins and bioactive compounds extracted from grape pomace and edible mushrooms (RS-ScienceFundRS-Ideje-7744714)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Kilibarda, Sofija AU - Milinčić, Danijel AU - Pantović, Jelena AU - Vuković, Sandra AU - Moravčević, Đorđe AU - Pešić, Mirjana AU - Kostić, Aleksandar Ž. PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6840 AB - Wild rocket, scientifically known as Diplotaxis tenuifolia (L.) DC is a leafy green plant from the Brassicaceae family. Native to the Medditerian region, today this perennial plant is cultivated worldwide, owing to the distinctive pungent taste of its deeply lobed green leaves. Beyond their culinary uses, the leaves of wild rocket exhibit potential for pharmaceutical applications due to their rich bioactive compounds profile. These include vitamins, pigments (chlorophyll a and b, carotenoids), phenolics, and glucosinolates (GLSs), all exhibiting strong antioxidant and anti-cancer activities. This study aimed to evaluate the effects of biofortification, employing biostimulant Kelpak and two distinct foliar fertilizers (iron and potassium-enriched) in comparison to a control group, on the phenolic and glucosinolate content in the leaves of wild rocket hybrid T&T F1. Using an ultra-highperformance liquid chromatography (UHPLC) system, coupled with a quadrupole time-offlight mass spectrometry (Q-ToF-MS), a total of twenty-three plant phenolics was quantified, and the relative content of four glucosinolates was evaluated in 70% methanol extracts. Concerning the overall content of phenolic compounds, treatment involving iron application showed the highest levels at 3549.1 mg/kg of fresh weight (FW), while the control group exhibited the lowest content (2171.4 mg/kg FW). Moreover, the most prominent phenolics included quercetin-3,4'-di-O-hexoside-3'-O-(6"-sinapoyl)-hexoside (ranging from 527.2 to 651.8 mg/kg FW among groups) and 1,8-dipropoxyanthraquinone (278.3-558.5 mg/kg FW). The control and iron-enriched samples exhibited the presence of kaempferol-3,7,4'-tri-Ohexoside + HCOOH and isorhamnetin-3-O-hexoside-4'-O-gentobioside, in addition to the non-identified sinapic acid hydroxide in the Kelpak treated group. Additionally, the determination of relative GLSs content of glucosativin, glucoerucin, neoglucobrassicin, and DMB-GLS revealed that glucosativin was the dominant compound, with proportions ranging from 79.8% (Kelpak treatments) to 89.1% (iron treatments). In conclusion, selected hybrid biofortification treatments, notably through the use of iron foliar fertilizers, enhanced phenolic and glucosinolate leaf content, highlighting its potential significance in nutrition and medicine. PB - UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY T2 - International Conference on Biochemical Engineering and Biotechnology for Young Scientists T1 - Comparative analysis of biofortification effects on phenolic and glucosinolate profiles in Diploptaxis tenuifolia (L.) DC T&T hybrid EP - 13 SP - 13 UR - https://hdl.handle.net/21.15107/rcub_agrospace_6840 ER -
@article{ author = "Kilibarda, Sofija and Milinčić, Danijel and Pantović, Jelena and Vuković, Sandra and Moravčević, Đorđe and Pešić, Mirjana and Kostić, Aleksandar Ž.", year = "2023", abstract = "Wild rocket, scientifically known as Diplotaxis tenuifolia (L.) DC is a leafy green plant from the Brassicaceae family. Native to the Medditerian region, today this perennial plant is cultivated worldwide, owing to the distinctive pungent taste of its deeply lobed green leaves. Beyond their culinary uses, the leaves of wild rocket exhibit potential for pharmaceutical applications due to their rich bioactive compounds profile. These include vitamins, pigments (chlorophyll a and b, carotenoids), phenolics, and glucosinolates (GLSs), all exhibiting strong antioxidant and anti-cancer activities. This study aimed to evaluate the effects of biofortification, employing biostimulant Kelpak and two distinct foliar fertilizers (iron and potassium-enriched) in comparison to a control group, on the phenolic and glucosinolate content in the leaves of wild rocket hybrid T&T F1. Using an ultra-highperformance liquid chromatography (UHPLC) system, coupled with a quadrupole time-offlight mass spectrometry (Q-ToF-MS), a total of twenty-three plant phenolics was quantified, and the relative content of four glucosinolates was evaluated in 70% methanol extracts. Concerning the overall content of phenolic compounds, treatment involving iron application showed the highest levels at 3549.1 mg/kg of fresh weight (FW), while the control group exhibited the lowest content (2171.4 mg/kg FW). Moreover, the most prominent phenolics included quercetin-3,4'-di-O-hexoside-3'-O-(6"-sinapoyl)-hexoside (ranging from 527.2 to 651.8 mg/kg FW among groups) and 1,8-dipropoxyanthraquinone (278.3-558.5 mg/kg FW). The control and iron-enriched samples exhibited the presence of kaempferol-3,7,4'-tri-Ohexoside + HCOOH and isorhamnetin-3-O-hexoside-4'-O-gentobioside, in addition to the non-identified sinapic acid hydroxide in the Kelpak treated group. Additionally, the determination of relative GLSs content of glucosativin, glucoerucin, neoglucobrassicin, and DMB-GLS revealed that glucosativin was the dominant compound, with proportions ranging from 79.8% (Kelpak treatments) to 89.1% (iron treatments). In conclusion, selected hybrid biofortification treatments, notably through the use of iron foliar fertilizers, enhanced phenolic and glucosinolate leaf content, highlighting its potential significance in nutrition and medicine.", publisher = "UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY", journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists", title = "Comparative analysis of biofortification effects on phenolic and glucosinolate profiles in Diploptaxis tenuifolia (L.) DC T&T hybrid", pages = "13-13", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6840" }
Kilibarda, S., Milinčić, D., Pantović, J., Vuković, S., Moravčević, Đ., Pešić, M.,& Kostić, A. Ž.. (2023). Comparative analysis of biofortification effects on phenolic and glucosinolate profiles in Diploptaxis tenuifolia (L.) DC T&T hybrid. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY., 13-13. https://hdl.handle.net/21.15107/rcub_agrospace_6840
Kilibarda S, Milinčić D, Pantović J, Vuković S, Moravčević Đ, Pešić M, Kostić AŽ. Comparative analysis of biofortification effects on phenolic and glucosinolate profiles in Diploptaxis tenuifolia (L.) DC T&T hybrid. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists. 2023;:13-13. https://hdl.handle.net/21.15107/rcub_agrospace_6840 .
Kilibarda, Sofija, Milinčić, Danijel, Pantović, Jelena, Vuković, Sandra, Moravčević, Đorđe, Pešić, Mirjana, Kostić, Aleksandar Ž., "Comparative analysis of biofortification effects on phenolic and glucosinolate profiles in Diploptaxis tenuifolia (L.) DC T&T hybrid" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists (2023):13-13, https://hdl.handle.net/21.15107/rcub_agrospace_6840 .