Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity
Аутори
Dragojević, MilicaĐokić, Lidija
Stanković, Nada
Raičević, Vera
Karličič, Vera
Lalević, Blažo
Jovičić-Petrović, Jelena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Increased soil salinity has numerous negative effects on plant growth and poses
a significant threat to agricultural productivity worldwide. The diversity of
endophytic bacterial communities found in various indigenous halophytes
such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may
reveal beneficial bacteria with various plant growthpromoting (PGP) properties.
Members of Halomonas, the largest genus of the family Halomonadaceae,
are rod-shaped, gram-negative, moderately halophilic bacteria that have been
increasingly isolated from various saline environments, including halophyte
tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting
endophytic bacteria can contribute to plant host growth, productivity, and
fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria
represents a good alternative strategy to mitigate the effects of salt stress in
crops and to recover saline soils. The aim of this study was to... isolate halotolerant
bacteria from halophyte roots, and evaluate their PGP ability and their effect on
germination parameters of selected crops. The endophytic bacterial communities
in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia)
were analyzed using culture-dependent techniques. The genus Halomonas was
the predominant among the halotolerant root-associated bacteria of analyzed
halophytes. We have isolated seven Halomonas sp. strains, six of which were
closely related (>99,86% similarity) to Halomonas songnenensis, a moderately
halophilic bacterium from saline and alkaline soils. Obtained isolates were tested
at different sodium chloride concentrations for a range of PGP traits at
different concentrations of sodium chloride. The selected strains were used to
inoculate seeds iand monitor of the germination process of barley, sunflower,
and wheat under conditions of elevated salinity. Most of the isolates tolerate
the NaCl concentration up to 18% and showed multiple PGP features with some
of them retained even in the presence of additional salt concentrations. The
germination test results showed that Halomonas seed inoculation positively
affected barley germination parameters in conditions with and without added
salt. The observed traits can be further exploited to improve plant growth and
augment the salt tolerance of agricultural production on saline soils.
Кључне речи:
halophytes / Halomonas / endophytesИзвор:
ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE, 2023Институција/група
Poljoprivredni fakultetTY - CONF AU - Dragojević, Milica AU - Đokić, Lidija AU - Stanković, Nada AU - Raičević, Vera AU - Karličič, Vera AU - Lalević, Blažo AU - Jovičić-Petrović, Jelena PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6605 AB - Increased soil salinity has numerous negative effects on plant growth and poses a significant threat to agricultural productivity worldwide. The diversity of endophytic bacterial communities found in various indigenous halophytes such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may reveal beneficial bacteria with various plant growthpromoting (PGP) properties. Members of Halomonas, the largest genus of the family Halomonadaceae, are rod-shaped, gram-negative, moderately halophilic bacteria that have been increasingly isolated from various saline environments, including halophyte tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting endophytic bacteria can contribute to plant host growth, productivity, and fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria represents a good alternative strategy to mitigate the effects of salt stress in crops and to recover saline soils. The aim of this study was to isolate halotolerant bacteria from halophyte roots, and evaluate their PGP ability and their effect on germination parameters of selected crops. The endophytic bacterial communities in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia) were analyzed using culture-dependent techniques. The genus Halomonas was the predominant among the halotolerant root-associated bacteria of analyzed halophytes. We have isolated seven Halomonas sp. strains, six of which were closely related (>99,86% similarity) to Halomonas songnenensis, a moderately halophilic bacterium from saline and alkaline soils. Obtained isolates were tested at different sodium chloride concentrations for a range of PGP traits at different concentrations of sodium chloride. The selected strains were used to inoculate seeds iand monitor of the germination process of barley, sunflower, and wheat under conditions of elevated salinity. Most of the isolates tolerate the NaCl concentration up to 18% and showed multiple PGP features with some of them retained even in the presence of additional salt concentrations. The germination test results showed that Halomonas seed inoculation positively affected barley germination parameters in conditions with and without added salt. The observed traits can be further exploited to improve plant growth and augment the salt tolerance of agricultural production on saline soils. C3 - ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE T1 - Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity UR - https://hdl.handle.net/21.15107/rcub_agrospace_6605 ER -
@conference{ author = "Dragojević, Milica and Đokić, Lidija and Stanković, Nada and Raičević, Vera and Karličič, Vera and Lalević, Blažo and Jovičić-Petrović, Jelena", year = "2023", abstract = "Increased soil salinity has numerous negative effects on plant growth and poses a significant threat to agricultural productivity worldwide. The diversity of endophytic bacterial communities found in various indigenous halophytes such as Salicornia europaea, Suaeda maritima, and Camphorosma annua, may reveal beneficial bacteria with various plant growthpromoting (PGP) properties. Members of Halomonas, the largest genus of the family Halomonadaceae, are rod-shaped, gram-negative, moderately halophilic bacteria that have been increasingly isolated from various saline environments, including halophyte tissues. As part of the halophytic microbiota, halotolerant plant growth-promoting endophytic bacteria can contribute to plant host growth, productivity, and fitness under abiotic and biotic stresses. The use of halotolerant PGP bacteria represents a good alternative strategy to mitigate the effects of salt stress in crops and to recover saline soils. The aim of this study was to isolate halotolerant bacteria from halophyte roots, and evaluate their PGP ability and their effect on germination parameters of selected crops. The endophytic bacterial communities in the roots of halophytes from natural saline soil in Slano Kopovo (Serbia) were analyzed using culture-dependent techniques. The genus Halomonas was the predominant among the halotolerant root-associated bacteria of analyzed halophytes. We have isolated seven Halomonas sp. strains, six of which were closely related (>99,86% similarity) to Halomonas songnenensis, a moderately halophilic bacterium from saline and alkaline soils. Obtained isolates were tested at different sodium chloride concentrations for a range of PGP traits at different concentrations of sodium chloride. The selected strains were used to inoculate seeds iand monitor of the germination process of barley, sunflower, and wheat under conditions of elevated salinity. Most of the isolates tolerate the NaCl concentration up to 18% and showed multiple PGP features with some of them retained even in the presence of additional salt concentrations. The germination test results showed that Halomonas seed inoculation positively affected barley germination parameters in conditions with and without added salt. The observed traits can be further exploited to improve plant growth and augment the salt tolerance of agricultural production on saline soils.", journal = "ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE", title = "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6605" }
Dragojević, M., Đokić, L., Stanković, N., Raičević, V., Karličič, V., Lalević, B.,& Jovičić-Petrović, J.. (2023). Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. https://hdl.handle.net/21.15107/rcub_agrospace_6605
Dragojević M, Đokić L, Stanković N, Raičević V, Karličič V, Lalević B, Jovičić-Petrović J. Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity. in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE. 2023;. https://hdl.handle.net/21.15107/rcub_agrospace_6605 .
Dragojević, Milica, Đokić, Lidija, Stanković, Nada, Raičević, Vera, Karličič, Vera, Lalević, Blažo, Jovičić-Petrović, Jelena, "Plant growth promoting Halomonas from roots of halophytes as a strategy to improve crop resistance to soil salinity" in ICGEB WORKSHOP, TRENDS IN MICROBIAL SOLUTIONS FOR SUSTAINABLE AGRICULTURE (2023), https://hdl.handle.net/21.15107/rcub_agrospace_6605 .