ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus
Autori
Kozarski, Maja S.Klaus, Anita S.
Lazić, Vesna V.
Stevanović, Snežana M.
Jakovljević, Dragica M.
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Phellinus linteus is a popular medicinal mushroom that is widely used in Asian countries. A number
of studies have confirmed that P. linteus possesses exceptional biological potential useful for
pharmacological applications, including anticancer and anti-inflammatory activities, as well as
antidiabetic, hepatoprotective, and neuroprotective effects. The objective of the present study was to
evaluate antioxidant and immunomodulatory activities of hot water polysaccharide extract obtained
from the medicinal mushroom Phellinus linteus (Berk. et Curt.) Teng. FT-IR was used to study the
polysaccharide profile of the extract. Its antioxidant potential was measured by the conjugated
diene method in the linoleic acid model system. Immunomodulation was tested in vitro by
measuring the synthesis of interferon-gamma (IFN-γ) in healthy human peripheral blood
mononuclear cells (PBMCs) using enzyme linked immunosorbent assay (ELISA). The FT-IR
spectrum of P. linteus hot water extracted pol...ysaccharides showed a typical carbohydrate pattern. A
small amount of proteins was also observed with characteristic absorptions at 1635, 1540 and 1412
cm-1
. Measurements of antioxidant properties in linoleic acid model system revealed relatively high
antioxidant activity with EC50 value of 7.11 mg/mL. After 48 h of P. linteus polysaccharide extract
incubation, the IFN-γ titer displayed immunosuppressive effect, 32.6 pg/mL. The IFN-γ titer for the
suspension of PBMCs in PBS, which was used as a positive control, was found to be 135.2 pg/mL.
Differences in IFN- γ contents in P. linteus extract vs. model control were strongly significant (p <
0.05). The results of this study suggest that the polysaccharide extract of P. linteus acts as a natural
antioxidant and possesses immunomodulatory properties. Therefore, it can be a suitable raw
material for the development of antioxidant food additives. In addition, due to the possible
immunosuppressive effect P. linteus polysaccharide extract is particularly interesting and could find
application in suppression of autoimmune diseases such as rheumatoid arthritis.
Ključne reči:
Phellinus linteus / Medicinal mushroom / Antioxidant / ImmunomodulationIzvor:
2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online, 2021Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200116 (Univerzitet u Beogradu, Poljoprivredni fakultet) (RS-MESTD-inst-2020-200116)
Institucija/grupa
Poljoprivredni fakultetTY - CONF AU - Kozarski, Maja S. AU - Klaus, Anita S. AU - Lazić, Vesna V. AU - Stevanović, Snežana M. AU - Jakovljević, Dragica M. PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6515 AB - Phellinus linteus is a popular medicinal mushroom that is widely used in Asian countries. A number of studies have confirmed that P. linteus possesses exceptional biological potential useful for pharmacological applications, including anticancer and anti-inflammatory activities, as well as antidiabetic, hepatoprotective, and neuroprotective effects. The objective of the present study was to evaluate antioxidant and immunomodulatory activities of hot water polysaccharide extract obtained from the medicinal mushroom Phellinus linteus (Berk. et Curt.) Teng. FT-IR was used to study the polysaccharide profile of the extract. Its antioxidant potential was measured by the conjugated diene method in the linoleic acid model system. Immunomodulation was tested in vitro by measuring the synthesis of interferon-gamma (IFN-γ) in healthy human peripheral blood mononuclear cells (PBMCs) using enzyme linked immunosorbent assay (ELISA). The FT-IR spectrum of P. linteus hot water extracted polysaccharides showed a typical carbohydrate pattern. A small amount of proteins was also observed with characteristic absorptions at 1635, 1540 and 1412 cm-1 . Measurements of antioxidant properties in linoleic acid model system revealed relatively high antioxidant activity with EC50 value of 7.11 mg/mL. After 48 h of P. linteus polysaccharide extract incubation, the IFN-γ titer displayed immunosuppressive effect, 32.6 pg/mL. The IFN-γ titer for the suspension of PBMCs in PBS, which was used as a positive control, was found to be 135.2 pg/mL. Differences in IFN- γ contents in P. linteus extract vs. model control were strongly significant (p < 0.05). The results of this study suggest that the polysaccharide extract of P. linteus acts as a natural antioxidant and possesses immunomodulatory properties. Therefore, it can be a suitable raw material for the development of antioxidant food additives. In addition, due to the possible immunosuppressive effect P. linteus polysaccharide extract is particularly interesting and could find application in suppression of autoimmune diseases such as rheumatoid arthritis. C3 - 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online T1 - ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus DO - 10.1016/j.foodchem.2011.06.029 ER -
@conference{ author = "Kozarski, Maja S. and Klaus, Anita S. and Lazić, Vesna V. and Stevanović, Snežana M. and Jakovljević, Dragica M.", year = "2021", abstract = "Phellinus linteus is a popular medicinal mushroom that is widely used in Asian countries. A number of studies have confirmed that P. linteus possesses exceptional biological potential useful for pharmacological applications, including anticancer and anti-inflammatory activities, as well as antidiabetic, hepatoprotective, and neuroprotective effects. The objective of the present study was to evaluate antioxidant and immunomodulatory activities of hot water polysaccharide extract obtained from the medicinal mushroom Phellinus linteus (Berk. et Curt.) Teng. FT-IR was used to study the polysaccharide profile of the extract. Its antioxidant potential was measured by the conjugated diene method in the linoleic acid model system. Immunomodulation was tested in vitro by measuring the synthesis of interferon-gamma (IFN-γ) in healthy human peripheral blood mononuclear cells (PBMCs) using enzyme linked immunosorbent assay (ELISA). The FT-IR spectrum of P. linteus hot water extracted polysaccharides showed a typical carbohydrate pattern. A small amount of proteins was also observed with characteristic absorptions at 1635, 1540 and 1412 cm-1 . Measurements of antioxidant properties in linoleic acid model system revealed relatively high antioxidant activity with EC50 value of 7.11 mg/mL. After 48 h of P. linteus polysaccharide extract incubation, the IFN-γ titer displayed immunosuppressive effect, 32.6 pg/mL. The IFN-γ titer for the suspension of PBMCs in PBS, which was used as a positive control, was found to be 135.2 pg/mL. Differences in IFN- γ contents in P. linteus extract vs. model control were strongly significant (p < 0.05). The results of this study suggest that the polysaccharide extract of P. linteus acts as a natural antioxidant and possesses immunomodulatory properties. Therefore, it can be a suitable raw material for the development of antioxidant food additives. In addition, due to the possible immunosuppressive effect P. linteus polysaccharide extract is particularly interesting and could find application in suppression of autoimmune diseases such as rheumatoid arthritis.", journal = "2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online", title = "ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus", doi = "10.1016/j.foodchem.2011.06.029" }
Kozarski, M. S., Klaus, A. S., Lazić, V. V., Stevanović, S. M.,& Jakovljević, D. M.. (2021). ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus. in 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online. https://doi.org/10.1016/j.foodchem.2011.06.029
Kozarski MS, Klaus AS, Lazić VV, Stevanović SM, Jakovljević DM. ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus. in 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online. 2021;. doi:10.1016/j.foodchem.2011.06.029 .
Kozarski, Maja S., Klaus, Anita S., Lazić, Vesna V., Stevanović, Snežana M., Jakovljević, Dragica M., "ANTIOXIDATIVE AND IMMUNOMODULATING POTENTIAL OF THE MUSHROOM Phellinus linteus" in 2nd International UNIFood Conference, University of Belgrade, 2nd International UNIfood Conference online (2021), https://doi.org/10.1016/j.foodchem.2011.06.029 . .