Raman microscopy/spectroscopy: non-destructive tool for the characterization of tomato fruit qualit
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Raman microscopy/spectroscopy is a method for non-destructive qualitative and quantitative
sample analysis and have recently been used for analysing of compounds in plant materials.
The aim of presenting study was to investigate potential of Raman spectroscopy in the
tomato fruit quality characterization, with special attention to carotenoids and vitamin C
analysis.
An experiment was conducted in plant growth chamber in controlled conditions at the Faculty
of Agriculture, University of Belgrade. Fruits of wild-type tomato Ailsa Craig and its mutant
flacca were harvested in red-ripe stage and sliced with microtome blades. Slices were
analysed by Raman spectroscopy (Raman Horiba XploRA), with a laser excitation lines 532 and
785 nm and 1-5 s integration time. The filter was set to 100 % and we used 100 x LWD.
The results indicated that Raman spectroscopy has the great potential for fast, nondestructive analysis of fruit quality, especially of carotenoid content. Analysis of car...otenoids
resulted in several strong peaks which could be also used for screening different tomato
genotypes both in optimal and stress conditions. Thus, Raman spectroscopy could be a useful
tool for fruit phenotyping.
Keywords:
Raman spectroscopy / tomato / fruit phenotypingSource:
COST WG1/EPPN2020 workshop. Current and future applications of phenotyping for plant breeding, Novi Sad, Serbia, 29- 30. September, 2017, Abstract book, 2017, 52-Publisher:
- Institute of Field and Vegetable Crops, Novi Sad, Serbia
Funding / projects:
- Biotechnological approaches for overcoming effects of drought on agricultural production in Serbia (RS-MESTD-Technological Development (TD or TR)-31005)
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Poljoprivredni fakultetTY - CONF AU - Petrović, Ivana AU - Stikić, Radmila AU - Jovanović, Zorica PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6499 AB - Raman microscopy/spectroscopy is a method for non-destructive qualitative and quantitative sample analysis and have recently been used for analysing of compounds in plant materials. The aim of presenting study was to investigate potential of Raman spectroscopy in the tomato fruit quality characterization, with special attention to carotenoids and vitamin C analysis. An experiment was conducted in plant growth chamber in controlled conditions at the Faculty of Agriculture, University of Belgrade. Fruits of wild-type tomato Ailsa Craig and its mutant flacca were harvested in red-ripe stage and sliced with microtome blades. Slices were analysed by Raman spectroscopy (Raman Horiba XploRA), with a laser excitation lines 532 and 785 nm and 1-5 s integration time. The filter was set to 100 % and we used 100 x LWD. The results indicated that Raman spectroscopy has the great potential for fast, nondestructive analysis of fruit quality, especially of carotenoid content. Analysis of carotenoids resulted in several strong peaks which could be also used for screening different tomato genotypes both in optimal and stress conditions. Thus, Raman spectroscopy could be a useful tool for fruit phenotyping. PB - Institute of Field and Vegetable Crops, Novi Sad, Serbia C3 - COST WG1/EPPN2020 workshop. Current and future applications of phenotyping for plant breeding, Novi Sad, Serbia, 29- 30. September, 2017, Abstract book T1 - Raman microscopy/spectroscopy: non-destructive tool for the characterization of tomato fruit qualit SP - 52 UR - https://hdl.handle.net/21.15107/rcub_agrospace_6499 ER -
@conference{ author = "Petrović, Ivana and Stikić, Radmila and Jovanović, Zorica", year = "2017", abstract = "Raman microscopy/spectroscopy is a method for non-destructive qualitative and quantitative sample analysis and have recently been used for analysing of compounds in plant materials. The aim of presenting study was to investigate potential of Raman spectroscopy in the tomato fruit quality characterization, with special attention to carotenoids and vitamin C analysis. An experiment was conducted in plant growth chamber in controlled conditions at the Faculty of Agriculture, University of Belgrade. Fruits of wild-type tomato Ailsa Craig and its mutant flacca were harvested in red-ripe stage and sliced with microtome blades. Slices were analysed by Raman spectroscopy (Raman Horiba XploRA), with a laser excitation lines 532 and 785 nm and 1-5 s integration time. The filter was set to 100 % and we used 100 x LWD. The results indicated that Raman spectroscopy has the great potential for fast, nondestructive analysis of fruit quality, especially of carotenoid content. Analysis of carotenoids resulted in several strong peaks which could be also used for screening different tomato genotypes both in optimal and stress conditions. Thus, Raman spectroscopy could be a useful tool for fruit phenotyping.", publisher = "Institute of Field and Vegetable Crops, Novi Sad, Serbia", journal = "COST WG1/EPPN2020 workshop. Current and future applications of phenotyping for plant breeding, Novi Sad, Serbia, 29- 30. September, 2017, Abstract book", title = "Raman microscopy/spectroscopy: non-destructive tool for the characterization of tomato fruit qualit", pages = "52", url = "https://hdl.handle.net/21.15107/rcub_agrospace_6499" }
Petrović, I., Stikić, R.,& Jovanović, Z.. (2017). Raman microscopy/spectroscopy: non-destructive tool for the characterization of tomato fruit qualit. in COST WG1/EPPN2020 workshop. Current and future applications of phenotyping for plant breeding, Novi Sad, Serbia, 29- 30. September, 2017, Abstract book Institute of Field and Vegetable Crops, Novi Sad, Serbia., 52. https://hdl.handle.net/21.15107/rcub_agrospace_6499
Petrović I, Stikić R, Jovanović Z. Raman microscopy/spectroscopy: non-destructive tool for the characterization of tomato fruit qualit. in COST WG1/EPPN2020 workshop. Current and future applications of phenotyping for plant breeding, Novi Sad, Serbia, 29- 30. September, 2017, Abstract book. 2017;:52. https://hdl.handle.net/21.15107/rcub_agrospace_6499 .
Petrović, Ivana, Stikić, Radmila, Jovanović, Zorica, "Raman microscopy/spectroscopy: non-destructive tool for the characterization of tomato fruit qualit" in COST WG1/EPPN2020 workshop. Current and future applications of phenotyping for plant breeding, Novi Sad, Serbia, 29- 30. September, 2017, Abstract book (2017):52, https://hdl.handle.net/21.15107/rcub_agrospace_6499 .