Application of molecular methods in weed science
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Molecular methods are useful tools for weed science, especially in the area of
weed resistance to herbicides and gene flow from herbicide tolerant crops to
their wild relatives. Also, genetic variability plays an important role in weed
susceptibility to herbicides and affect on strategies of control. For all of these
studies, DNA, as a starting material, could be extracted by various methods;
though, the easiest and the most suitable is extraction by using commercially
available kits. The most important part of molecular analysis is selection and
design of adequate primers for successful DNA amplification. Usually, primer
selection and designing are based on DNA sequences stored in GenBank. Analysis following selected DNA fragments will depend on type of research. For
weed resistance or gene flow studies, amplified fragments are sequenced and
obtained information compared with the GenBank sequence database, with the
aim to check for mutation(s) presence. For genetic diversit...y of weed species
analysis of amplified DNA fragments include Capillary Electrophoresis.
Извор:
2019, 15-22Финансирање / пројекти:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
URI
https://www.researchgate.net/publication/334640548_Application_of_molecular_methods_in_weed_sciencehttp://aspace.agrif.bg.ac.rs/handle/123456789/6436
Институција/група
Poljoprivredni fakultetBozić, D., Saulić, M.,& Vrbničanin, S.. (2019). Application of molecular methods in weed science. , 15-22. https://doi.org/10.5334/bbj.b/
Bozić D, Saulić M, Vrbničanin S. Application of molecular methods in weed science. 2019;:15-22. doi:10.5334/bbj.b/ .
Bozić, Dragana, Saulić, Markola, Vrbničanin, Sava, "Application of molecular methods in weed science" (2019):15-22, https://doi.org/10.5334/bbj.b/ . .