Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers
Аутори
Gasi, FuadPojskić, Naris
Stroil, Belma Kalamuji´c
Frøynes, Oddmund
Fotirić Akšić, Milica
Meland, Mekjell
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In order to determine the pollinizer success rates between twelve apple cultivars in 2021 and 2022, 671 apple embryos were collected from 19 different orchards in Ullensvang (southwestern Norway) and Svelvik (southeastern Norway). Genomic DNA was extracted from the collected embryos and, afterward, a genetic characterization with 15 polymorphic microsatellite markers was conducted. An identical set of markers was also used on all twelve mother cultivars, as well as on six crabapple pollinizers, which were found in the investigated orchards. The obtained molecular data enabled paternity analyses to be performed with the objective of assigning a male parent to each embryo. The paternity analyses identified pollen donors for all, except for 3% of the embryos. In most cases, it was possible to identify the most successful pollinizers for each cultivar, with ‘Aroma’ and ‘Discovery’ being the most efficient pollen donors overall. Tree abundance seems to be a major factor in pollinizer succes...s, while semi-cross-compatible characteristics represent a hindrance. Only 7% of the analyzed embryos were determined to have been fertilized by pollinizers outside the orchard, confirming the significance of pollinizer proximity for efficient pollination. © 2023 by the authors.
Кључне речи:
cross compatibility / embryos / paternity analyses / pollinizers / SSR / tree abundanceИзвор:
Agronomy, 2023, 13, 4Институција/група
Poljoprivredni fakultetTY - JOUR AU - Gasi, Fuad AU - Pojskić, Naris AU - Stroil, Belma Kalamuji´c AU - Frøynes, Oddmund AU - Fotirić Akšić, Milica AU - Meland, Mekjell PY - 2023 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6341 AB - In order to determine the pollinizer success rates between twelve apple cultivars in 2021 and 2022, 671 apple embryos were collected from 19 different orchards in Ullensvang (southwestern Norway) and Svelvik (southeastern Norway). Genomic DNA was extracted from the collected embryos and, afterward, a genetic characterization with 15 polymorphic microsatellite markers was conducted. An identical set of markers was also used on all twelve mother cultivars, as well as on six crabapple pollinizers, which were found in the investigated orchards. The obtained molecular data enabled paternity analyses to be performed with the objective of assigning a male parent to each embryo. The paternity analyses identified pollen donors for all, except for 3% of the embryos. In most cases, it was possible to identify the most successful pollinizers for each cultivar, with ‘Aroma’ and ‘Discovery’ being the most efficient pollen donors overall. Tree abundance seems to be a major factor in pollinizer success, while semi-cross-compatible characteristics represent a hindrance. Only 7% of the analyzed embryos were determined to have been fertilized by pollinizers outside the orchard, confirming the significance of pollinizer proximity for efficient pollination. © 2023 by the authors. T2 - Agronomy T2 - Agronomy T1 - Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers IS - 4 VL - 13 DO - 10.3390/agronomy13041106 ER -
@article{ author = "Gasi, Fuad and Pojskić, Naris and Stroil, Belma Kalamuji´c and Frøynes, Oddmund and Fotirić Akšić, Milica and Meland, Mekjell", year = "2023", abstract = "In order to determine the pollinizer success rates between twelve apple cultivars in 2021 and 2022, 671 apple embryos were collected from 19 different orchards in Ullensvang (southwestern Norway) and Svelvik (southeastern Norway). Genomic DNA was extracted from the collected embryos and, afterward, a genetic characterization with 15 polymorphic microsatellite markers was conducted. An identical set of markers was also used on all twelve mother cultivars, as well as on six crabapple pollinizers, which were found in the investigated orchards. The obtained molecular data enabled paternity analyses to be performed with the objective of assigning a male parent to each embryo. The paternity analyses identified pollen donors for all, except for 3% of the embryos. In most cases, it was possible to identify the most successful pollinizers for each cultivar, with ‘Aroma’ and ‘Discovery’ being the most efficient pollen donors overall. Tree abundance seems to be a major factor in pollinizer success, while semi-cross-compatible characteristics represent a hindrance. Only 7% of the analyzed embryos were determined to have been fertilized by pollinizers outside the orchard, confirming the significance of pollinizer proximity for efficient pollination. © 2023 by the authors.", journal = "Agronomy, Agronomy", title = "Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers", number = "4", volume = "13", doi = "10.3390/agronomy13041106" }
Gasi, F., Pojskić, N., Stroil, B. K., Frøynes, O., Fotirić Akšić, M.,& Meland, M.. (2023). Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers. in Agronomy, 13(4). https://doi.org/10.3390/agronomy13041106
Gasi F, Pojskić N, Stroil BK, Frøynes O, Fotirić Akšić M, Meland M. Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers. in Agronomy. 2023;13(4). doi:10.3390/agronomy13041106 .
Gasi, Fuad, Pojskić, Naris, Stroil, Belma Kalamuji´c, Frøynes, Oddmund, Fotirić Akšić, Milica, Meland, Mekjell, "Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers" in Agronomy, 13, no. 4 (2023), https://doi.org/10.3390/agronomy13041106 . .