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dc.creatorStojanović, Sandra Stamenković
dc.creatorKarabegović, Ivana
dc.creatorDanilović, Bojana
dc.creatorNedović, Viktor
dc.creatorKalušević, Ana
dc.creatorMančić, Stojan
dc.creatorLazić, Miodrag
dc.date.accessioned2022-10-03T11:53:57Z
dc.date.available2022-10-03T11:53:57Z
dc.date.issued2022
dc.identifier.issn1695-971X
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/6178
dc.description.abstractAim of the study: This study aimed to formulate a novel, commercially applicable biofertilizer, to optimize the microencapsulation procedure of Bacillus subtilis NCIM 2063 and examine the stability and phytostimulatory effects of obtained formulation. Area of the study: Southestern Serbia. Material and methods: Microbial powder formulations were prepared using spray drying with maltodextrin as a carrier. The spray drying conditions were set according to Box-Benkhen experimental desing. The effect of the formulation was tested on green pepper (Capsicum annuum) seeds in controled conditions. Main results: Response surface models were developed. All of the models were statistically significant, adequately fitted and reproducible. The maximum achieved values of viability and yield in a formulation were 1.99·109 CFU/g and 96.8%, respectively, whilst the driest formulation had 1.44% moisture. The following optimum conditions were proposed for the spray drying procedure: an inlet air temperature of 133 °C, maltodextrin concentration of 50 g/L and a feed flow rate of 6.5 mL/min. The obtained microbial formulation had a high survival rate after being stored at room temperature over a 1--year period. Its application on green pepper seeds had beneficial effect on plant height, leaf dry weight and chlorophyll content of the seedlings. Research highlights: B. subtilis was successfully microencapsulated on maltodextrin as a carrier. Interaction effects between the process variables were fully explained and statistically significant models were developed. In addition to biocontrol properties formulation had a phytostimulatory effect, excellent stability and satisfactory physical properties. © 2022 CSIC.
dc.languageEnglish
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200133/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200116/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceSpanish Journal of Agricultural Research
dc.sourceSpanish Journal of Agricultural Research
dc.subjectAdditional plant growth promotion
dc.subjectBacillus subtilis
dc.subjectoptimization
dc.subjectphytostimulation
dc.titleMicroencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings
dc.typearticleen
dc.rights.licenseBY
dc.citation.issue3
dc.citation.rankM23
dc.citation.volume20
dc.identifier.doi10.5424/sjar/2022203-19062
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/24043/Microencapsulated_biofertilizer_formulation_pub_2022.pdf
dc.type.versionpublishedVersion


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