Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings
Authors
Stojanović, Sandra StamenkovićKarabegović, Ivana
Danilović, Bojana
Nedović, Viktor
Kalušević, Ana
Mančić, Stojan
Lazić, Miodrag
Article (Published version)
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Aim of the study: This study aimed to formulate a novel, commercially applicable biofertilizer, to optimize the microencapsulation procedure of Bacillus subtilis NCIM 2063 and examine the stability and phytostimulatory effects of obtained formulation. Area of the study: Southestern Serbia. Material and methods: Microbial powder formulations were prepared using spray drying with maltodextrin as a carrier. The spray drying conditions were set according to Box-Benkhen experimental desing. The effect of the formulation was tested on green pepper (Capsicum annuum) seeds in controled conditions. Main results: Response surface models were developed. All of the models were statistically significant, adequately fitted and reproducible. The maximum achieved values of viability and yield in a formulation were 1.99·109 CFU/g and 96.8%, respectively, whilst the driest formulation had 1.44% moisture. The following optimum conditions were proposed for the spray drying procedure: an inlet air temperat...ure of 133 °C, maltodextrin concentration of 50 g/L and a feed flow rate of 6.5 mL/min. The obtained microbial formulation had a high survival rate after being stored at room temperature over a 1--year period. Its application on green pepper seeds had beneficial effect on plant height, leaf dry weight and chlorophyll content of the seedlings. Research highlights: B. subtilis was successfully microencapsulated on maltodextrin as a carrier. Interaction effects between the process variables were fully explained and statistically significant models were developed. In addition to biocontrol properties formulation had a phytostimulatory effect, excellent stability and satisfactory physical properties. © 2022 CSIC.
Keywords:
Additional plant growth promotion / Bacillus subtilis / optimization / phytostimulationSource:
Spanish Journal of Agricultural Research, 2022, 20, 3Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200133 (Univeristy of Niš, Faculty of Technology, Leskovac) (RS-MESTD-inst-2020-200133)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
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Poljoprivredni fakultetTY - JOUR AU - Stojanović, Sandra Stamenković AU - Karabegović, Ivana AU - Danilović, Bojana AU - Nedović, Viktor AU - Kalušević, Ana AU - Mančić, Stojan AU - Lazić, Miodrag PY - 2022 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6178 AB - Aim of the study: This study aimed to formulate a novel, commercially applicable biofertilizer, to optimize the microencapsulation procedure of Bacillus subtilis NCIM 2063 and examine the stability and phytostimulatory effects of obtained formulation. Area of the study: Southestern Serbia. Material and methods: Microbial powder formulations were prepared using spray drying with maltodextrin as a carrier. The spray drying conditions were set according to Box-Benkhen experimental desing. The effect of the formulation was tested on green pepper (Capsicum annuum) seeds in controled conditions. Main results: Response surface models were developed. All of the models were statistically significant, adequately fitted and reproducible. The maximum achieved values of viability and yield in a formulation were 1.99·109 CFU/g and 96.8%, respectively, whilst the driest formulation had 1.44% moisture. The following optimum conditions were proposed for the spray drying procedure: an inlet air temperature of 133 °C, maltodextrin concentration of 50 g/L and a feed flow rate of 6.5 mL/min. The obtained microbial formulation had a high survival rate after being stored at room temperature over a 1--year period. Its application on green pepper seeds had beneficial effect on plant height, leaf dry weight and chlorophyll content of the seedlings. Research highlights: B. subtilis was successfully microencapsulated on maltodextrin as a carrier. Interaction effects between the process variables were fully explained and statistically significant models were developed. In addition to biocontrol properties formulation had a phytostimulatory effect, excellent stability and satisfactory physical properties. © 2022 CSIC. T2 - Spanish Journal of Agricultural Research T2 - Spanish Journal of Agricultural Research T1 - Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings IS - 3 VL - 20 DO - 10.5424/sjar/2022203-19062 ER -
@article{ author = "Stojanović, Sandra Stamenković and Karabegović, Ivana and Danilović, Bojana and Nedović, Viktor and Kalušević, Ana and Mančić, Stojan and Lazić, Miodrag", year = "2022", abstract = "Aim of the study: This study aimed to formulate a novel, commercially applicable biofertilizer, to optimize the microencapsulation procedure of Bacillus subtilis NCIM 2063 and examine the stability and phytostimulatory effects of obtained formulation. Area of the study: Southestern Serbia. Material and methods: Microbial powder formulations were prepared using spray drying with maltodextrin as a carrier. The spray drying conditions were set according to Box-Benkhen experimental desing. The effect of the formulation was tested on green pepper (Capsicum annuum) seeds in controled conditions. Main results: Response surface models were developed. All of the models were statistically significant, adequately fitted and reproducible. The maximum achieved values of viability and yield in a formulation were 1.99·109 CFU/g and 96.8%, respectively, whilst the driest formulation had 1.44% moisture. The following optimum conditions were proposed for the spray drying procedure: an inlet air temperature of 133 °C, maltodextrin concentration of 50 g/L and a feed flow rate of 6.5 mL/min. The obtained microbial formulation had a high survival rate after being stored at room temperature over a 1--year period. Its application on green pepper seeds had beneficial effect on plant height, leaf dry weight and chlorophyll content of the seedlings. Research highlights: B. subtilis was successfully microencapsulated on maltodextrin as a carrier. Interaction effects between the process variables were fully explained and statistically significant models were developed. In addition to biocontrol properties formulation had a phytostimulatory effect, excellent stability and satisfactory physical properties. © 2022 CSIC.", journal = "Spanish Journal of Agricultural Research, Spanish Journal of Agricultural Research", title = "Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings", number = "3", volume = "20", doi = "10.5424/sjar/2022203-19062" }
Stojanović, S. S., Karabegović, I., Danilović, B., Nedović, V., Kalušević, A., Mančić, S.,& Lazić, M.. (2022). Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings. in Spanish Journal of Agricultural Research, 20(3). https://doi.org/10.5424/sjar/2022203-19062
Stojanović SS, Karabegović I, Danilović B, Nedović V, Kalušević A, Mančić S, Lazić M. Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings. in Spanish Journal of Agricultural Research. 2022;20(3). doi:10.5424/sjar/2022203-19062 .
Stojanović, Sandra Stamenković, Karabegović, Ivana, Danilović, Bojana, Nedović, Viktor, Kalušević, Ana, Mančić, Stojan, Lazić, Miodrag, "Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings" in Spanish Journal of Agricultural Research, 20, no. 3 (2022), https://doi.org/10.5424/sjar/2022203-19062 . .