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Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits

Authorized Users Only
2022
Authors
Krstić, Đurđa
Milinčić, Danijel D.
Kostić, Aleksandar Ž.
Fotirić-Akšić, Milica
Stanojević, Slađana P.
Milojković-Opsenica, Dušanka
Pešić, Mirjana B.
Trifkovića, Jelena
Article (Published version)
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Abstract
Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-...based products adulteration.

Keywords:
Berry seeds / Chemometrics / Image analysis / Polypeptide profiling / SDS-PAGE electrophoresis
Source:
Food Chemistry, 2022, 383, 132583-
Publisher:
  • Elsevier Ltd
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)

DOI: 10.1016/j.foodchem.2022.132583

ISSN: 0308-8146

WoS: 00077828990001

Scopus: 2-s2.0-85125484658
[ Google Scholar ]
2
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/6039
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Poljoprivredni fakultet
TY  - JOUR
AU  - Krstić, Đurđa
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Fotirić-Akšić, Milica
AU  - Stanojević, Slađana P.
AU  - Milojković-Opsenica, Dušanka
AU  - Pešić, Mirjana B.
AU  - Trifkovića, Jelena
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6039
AB  - Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-based products adulteration.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits
SP  - 132583
VL  - 383
DO  - 10.1016/j.foodchem.2022.132583
ER  - 
@article{
author = "Krstić, Đurđa and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Fotirić-Akšić, Milica and Stanojević, Slađana P. and Milojković-Opsenica, Dušanka and Pešić, Mirjana B. and Trifkovića, Jelena",
year = "2022",
abstract = "Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-based products adulteration.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits",
pages = "132583",
volume = "383",
doi = "10.1016/j.foodchem.2022.132583"
}
Krstić, Đ., Milinčić, D. D., Kostić, A. Ž., Fotirić-Akšić, M., Stanojević, S. P., Milojković-Opsenica, D., Pešić, M. B.,& Trifkovića, J.. (2022). Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry
Elsevier Ltd., 383, 132583.
https://doi.org/10.1016/j.foodchem.2022.132583
Krstić Đ, Milinčić DD, Kostić AŽ, Fotirić-Akšić M, Stanojević SP, Milojković-Opsenica D, Pešić MB, Trifkovića J. Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry. 2022;383:132583.
doi:10.1016/j.foodchem.2022.132583 .
Krstić, Đurđa, Milinčić, Danijel D., Kostić, Aleksandar Ž., Fotirić-Akšić, Milica, Stanojević, Slađana P., Milojković-Opsenica, Dušanka, Pešić, Mirjana B., Trifkovića, Jelena, "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits" in Food Chemistry, 383 (2022):132583,
https://doi.org/10.1016/j.foodchem.2022.132583 . .

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