Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits
Authorized Users Only
2022
Authors
Krstić, Đurđa
Milinčić, Danijel D.

Kostić, Aleksandar Ž.

Fotirić-Akšić, Milica

Stanojević, Slađana P.

Milojković-Opsenica, Dušanka

Pešić, Mirjana B.

Trifkovića, Jelena
Article (Published version)

Metadata
Show full item recordAbstract
Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-...based products adulteration.
Keywords:
Berry seeds / Chemometrics / Image analysis / Polypeptide profiling / SDS-PAGE electrophoresisSource:
Food Chemistry, 2022, 383, 132583-Publisher:
- Elsevier Ltd
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
DOI: 10.1016/j.foodchem.2022.132583
ISSN: 0308-8146
WoS: 00077828990001
Scopus: 2-s2.0-85125484658
Collections
Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Krstić, Đurđa AU - Milinčić, Danijel D. AU - Kostić, Aleksandar Ž. AU - Fotirić-Akšić, Milica AU - Stanojević, Slađana P. AU - Milojković-Opsenica, Dušanka AU - Pešić, Mirjana B. AU - Trifkovića, Jelena PY - 2022 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6039 AB - Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-based products adulteration. PB - Elsevier Ltd T2 - Food Chemistry T1 - Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits SP - 132583 VL - 383 DO - 10.1016/j.foodchem.2022.132583 ER -
@article{ author = "Krstić, Đurđa and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Fotirić-Akšić, Milica and Stanojević, Slađana P. and Milojković-Opsenica, Dušanka and Pešić, Mirjana B. and Trifkovića, Jelena", year = "2022", abstract = "Product authentication is one of the most important food quality assurances. Considering the importance of consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars (strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4; 30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of berry-based products adulteration.", publisher = "Elsevier Ltd", journal = "Food Chemistry", title = "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits", pages = "132583", volume = "383", doi = "10.1016/j.foodchem.2022.132583" }
Krstić, Đ., Milinčić, D. D., Kostić, A. Ž., Fotirić-Akšić, M., Stanojević, S. P., Milojković-Opsenica, D., Pešić, M. B.,& Trifkovića, J.. (2022). Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry Elsevier Ltd., 383, 132583. https://doi.org/10.1016/j.foodchem.2022.132583
Krstić Đ, Milinčić DD, Kostić AŽ, Fotirić-Akšić M, Stanojević SP, Milojković-Opsenica D, Pešić MB, Trifkovića J. Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry. 2022;383:132583. doi:10.1016/j.foodchem.2022.132583 .
Krstić, Đurđa, Milinčić, Danijel D., Kostić, Aleksandar Ž., Fotirić-Akšić, Milica, Stanojević, Slađana P., Milojković-Opsenica, Dušanka, Pešić, Mirjana B., Trifkovića, Jelena, "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits" in Food Chemistry, 383 (2022):132583, https://doi.org/10.1016/j.foodchem.2022.132583 . .