Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
Autori
Zlatković, NevenaGašić, Katarina
Kuzmanović, Nemanja
Prokić, Anđelka
Ivanović, Milan
Živković, Svetlana
Obradović, Aleksa
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also c...onfirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.
Ključne reči:
bacterial fruit blotch / Acidovorax citrulli / watermelon / identification / MLSAIzvor:
Agronomy, 2022, 12, 2, 235-Izdavač:
- MDPI
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200010 (Institut za zaštitu bilja i životnu sredinu, Beograd) (RS-200010)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200116 (Univerzitet u Beogradu, Poljoprivredni fakultet) (RS-200116)
- The work of N.K. was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project Number 429677233.
DOI: 10.3390/agronomy12020235
ISSN: 2073-4395
WoS: 000762479700001
Scopus: 2-s2.0-85123164577
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Zlatković, Nevena AU - Gašić, Katarina AU - Kuzmanović, Nemanja AU - Prokić, Anđelka AU - Ivanović, Milan AU - Živković, Svetlana AU - Obradović, Aleksa PY - 2022 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/6024 AB - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family. PB - MDPI T2 - Agronomy T1 - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia IS - 2 SP - 235 VL - 12 DO - 10.3390/agronomy12020235 ER -
@article{ author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Živković, Svetlana and Obradović, Aleksa", year = "2022", abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.", publisher = "MDPI", journal = "Agronomy", title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia", number = "2", pages = "235", volume = "12", doi = "10.3390/agronomy12020235" }
Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Živković, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy MDPI., 12(2), 235. https://doi.org/10.3390/agronomy12020235
Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Živković S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12(2):235. doi:10.3390/agronomy12020235 .
Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Živković, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12, no. 2 (2022):235, https://doi.org/10.3390/agronomy12020235 . .