Using scanning electron microscopy to characterise plum (Prunus domestica L.) genotypes
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Pollen grain micromorphological characterisation of four plum (Prunus domestica L.) genotypes from two different breeding programmes (Fruit Research Institute, Serbia, and Hohenheim, Germany) was performed using scanning electron microscopy (SEM). The studied characteristics were pollen size and shape, colpus length, mesocolpium width as well as exine characteristics. All genotypes had tricolpate pollen grains and striate exine ornamentation, with more parallel longitudinal ridges. The most significant differences among the analysed genotypes concerned the length of the pollen grain and exine sculpturing. The genotypes 'Pozna Plava', 'Presenta' and 'Čačanska najbolja' had the largest pollen grains (> 57 µm), whereas 'Hanita' showed a smaller pollen size, below 54 µm. 'Pozna Plava' had perprolate pollen in the equatorial view (L/W ratio 2.06), while the other three genotypes had prolate shaped pollen. By analysing the pollen morphological characteristics of the plum varieties in this pa...per, primarily the shape, size, length of colpi and, most importantly, the sculpture of the exine, each variety showed a specificity which can be used together with other morphological characteristics for description and characterisation.
Keywords:European plum / Exine characteristics / Morphology / Pollen grains / Sculpture / SEM
Source:Comptes Rendus de L'Academie Bulgare des Sciences, 2021, 73, 10, 1390-1397
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