Resistance‑breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia
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2021
Authors
Petrović, BrankaVučurović, Ana
Zečević, Katarina
Delibašić, Goran
Krstić, Branka
Stanković, Ivana
Article (Published version)
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Show full item recordAbstract
The most effective management strategy for tomato spotted wilt orthotospovirus (TSWV) has been the use of resistant tomato (Sw-5b+) cultivars. Unfortunately, resistance-breaking isolates (RB) have emerged in many countries. In 2019, TSWV-like symptoms were observed in protected crops of a tomato (Sw-5b+) cultivar in two separate locations in Serbia. To identify variations associated with Serbian TSWV isolates that are able to infect resistant tomato cultivars, 11 samples with TSWV-like symptoms collected from susceptible (Sw-5b−) cultivars from 2011 to 2015, and three collected from the resistant cultivar in 2019 were tested. The presence of TSWV was confirmed serologically in all tomato samples, and partial nucleotide sequences of the NSm and N genes of 14 selected isolates were obtained. The NSm amino acid sequence comparison revealed the presence of two amino acid substitutions (C118Y or T120N) in three isolates originating from tomato (Sw-5b+) indicating their RB phenotype and inde...pendent evolutionary events. Isolates originating from tomato (Sw-5b−) cultivars were determined as resistance-inducing isolates (RI). NSm and N gene phylogenetic analyses showed the clustering of Serbian RB and RI isolates regardless of their phenotype. The N gene tree also showed the clustering of Serbian TSWV isolates in different subpopulations within the European clade indicating two independent introduction events and gene flow between southern and northern regions of the country. Bioassay confirmed that three RB isolates had the ability to break the Sw-5b-mediated resistance in tomato, but were unable to overcome the Tsw resistance gene in pepper. This first detection of the Sw-5b resistance-breaking in Serbia shows the necessity of further monitoring to prevent the establishment of RB TSWV isolates.
Keywords:
Biological and molecular characterization / Resistance-breaking isolates / Tomato / Tomato spotted wilt orthotospovirusSource:
Journal of Plant Diseases and Protection, 2021Publisher:
- Springer Science and Business Media Deutschland GmbH
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
DOI: 10.1007/s41348-021-00493-4
ISSN: 1861-3829
WoS: 000662800000002
Scopus: 2-s2.0-85108102768
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Poljoprivredni fakultetTY - JOUR AU - Petrović, Branka AU - Vučurović, Ana AU - Zečević, Katarina AU - Delibašić, Goran AU - Krstić, Branka AU - Stanković, Ivana PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5878 AB - The most effective management strategy for tomato spotted wilt orthotospovirus (TSWV) has been the use of resistant tomato (Sw-5b+) cultivars. Unfortunately, resistance-breaking isolates (RB) have emerged in many countries. In 2019, TSWV-like symptoms were observed in protected crops of a tomato (Sw-5b+) cultivar in two separate locations in Serbia. To identify variations associated with Serbian TSWV isolates that are able to infect resistant tomato cultivars, 11 samples with TSWV-like symptoms collected from susceptible (Sw-5b−) cultivars from 2011 to 2015, and three collected from the resistant cultivar in 2019 were tested. The presence of TSWV was confirmed serologically in all tomato samples, and partial nucleotide sequences of the NSm and N genes of 14 selected isolates were obtained. The NSm amino acid sequence comparison revealed the presence of two amino acid substitutions (C118Y or T120N) in three isolates originating from tomato (Sw-5b+) indicating their RB phenotype and independent evolutionary events. Isolates originating from tomato (Sw-5b−) cultivars were determined as resistance-inducing isolates (RI). NSm and N gene phylogenetic analyses showed the clustering of Serbian RB and RI isolates regardless of their phenotype. The N gene tree also showed the clustering of Serbian TSWV isolates in different subpopulations within the European clade indicating two independent introduction events and gene flow between southern and northern regions of the country. Bioassay confirmed that three RB isolates had the ability to break the Sw-5b-mediated resistance in tomato, but were unable to overcome the Tsw resistance gene in pepper. This first detection of the Sw-5b resistance-breaking in Serbia shows the necessity of further monitoring to prevent the establishment of RB TSWV isolates. PB - Springer Science and Business Media Deutschland GmbH T2 - Journal of Plant Diseases and Protection T1 - Resistance‑breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia DO - 10.1007/s41348-021-00493-4 ER -
@article{ author = "Petrović, Branka and Vučurović, Ana and Zečević, Katarina and Delibašić, Goran and Krstić, Branka and Stanković, Ivana", year = "2021", abstract = "The most effective management strategy for tomato spotted wilt orthotospovirus (TSWV) has been the use of resistant tomato (Sw-5b+) cultivars. Unfortunately, resistance-breaking isolates (RB) have emerged in many countries. In 2019, TSWV-like symptoms were observed in protected crops of a tomato (Sw-5b+) cultivar in two separate locations in Serbia. To identify variations associated with Serbian TSWV isolates that are able to infect resistant tomato cultivars, 11 samples with TSWV-like symptoms collected from susceptible (Sw-5b−) cultivars from 2011 to 2015, and three collected from the resistant cultivar in 2019 were tested. The presence of TSWV was confirmed serologically in all tomato samples, and partial nucleotide sequences of the NSm and N genes of 14 selected isolates were obtained. The NSm amino acid sequence comparison revealed the presence of two amino acid substitutions (C118Y or T120N) in three isolates originating from tomato (Sw-5b+) indicating their RB phenotype and independent evolutionary events. Isolates originating from tomato (Sw-5b−) cultivars were determined as resistance-inducing isolates (RI). NSm and N gene phylogenetic analyses showed the clustering of Serbian RB and RI isolates regardless of their phenotype. The N gene tree also showed the clustering of Serbian TSWV isolates in different subpopulations within the European clade indicating two independent introduction events and gene flow between southern and northern regions of the country. Bioassay confirmed that three RB isolates had the ability to break the Sw-5b-mediated resistance in tomato, but were unable to overcome the Tsw resistance gene in pepper. This first detection of the Sw-5b resistance-breaking in Serbia shows the necessity of further monitoring to prevent the establishment of RB TSWV isolates.", publisher = "Springer Science and Business Media Deutschland GmbH", journal = "Journal of Plant Diseases and Protection", title = "Resistance‑breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia", doi = "10.1007/s41348-021-00493-4" }
Petrović, B., Vučurović, A., Zečević, K., Delibašić, G., Krstić, B.,& Stanković, I.. (2021). Resistance‑breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia. in Journal of Plant Diseases and Protection Springer Science and Business Media Deutschland GmbH.. https://doi.org/10.1007/s41348-021-00493-4
Petrović B, Vučurović A, Zečević K, Delibašić G, Krstić B, Stanković I. Resistance‑breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia. in Journal of Plant Diseases and Protection. 2021;. doi:10.1007/s41348-021-00493-4 .
Petrović, Branka, Vučurović, Ana, Zečević, Katarina, Delibašić, Goran, Krstić, Branka, Stanković, Ivana, "Resistance‑breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia" in Journal of Plant Diseases and Protection (2021), https://doi.org/10.1007/s41348-021-00493-4 . .