Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor
Samo za registrovane korisnike
2021
Autori
Miletić, Dunja
Turło, Jadwiga
Podsadni, Piotr
Sknepnek, Aleksandra

Szczepanska, Agnieszka
Klimaszewska, Marzenna
Malinowska, Eliza
Lević, Steva

Nedović, Viktor

Nikšić, Miomir

Članak u časopisu (Objavljena verzija)

Metapodaci
Prikaz svih podataka o dokumentuApstrakt
During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. ...Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.
Ključne reči:
Antibacterial activity / Antioxidative activity / Exopolysaccharides / Mushrooms / Selenium / Submerged cultivationIzvor:
Food Bioscience, 2021, 42, 101046-Izdavač:
- Elsevier Ltd
Finansiranje / projekti:
DOI: 10.1016/j.fbio.2021.101046
ISSN: 2212-4292
WoS: 000687781400010
Scopus: 2-s2.0-85104050605
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Miletić, Dunja AU - Turło, Jadwiga AU - Podsadni, Piotr AU - Sknepnek, Aleksandra AU - Szczepanska, Agnieszka AU - Klimaszewska, Marzenna AU - Malinowska, Eliza AU - Lević, Steva AU - Nedović, Viktor AU - Nikšić, Miomir PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5836 AB - During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens. PB - Elsevier Ltd T2 - Food Bioscience T1 - Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor SP - 101046 VL - 42 DO - 10.1016/j.fbio.2021.101046 ER -
@article{ author = "Miletić, Dunja and Turło, Jadwiga and Podsadni, Piotr and Sknepnek, Aleksandra and Szczepanska, Agnieszka and Klimaszewska, Marzenna and Malinowska, Eliza and Lević, Steva and Nedović, Viktor and Nikšić, Miomir", year = "2021", abstract = "During a bioconversion to crude exopolysaccharides enriched with selenium from selenourea (SU-cEPS) and sodium selenite (SE-cEPS) in Trametes versicolor submerged culture, high selenium accumulation was achieved. The FTIR analysis detected two major fractions of selenium-cEPS samples: polysaccharides and proteins, indicating their proteoglycan character. Higher carbohydrate (595.98 ± 35.35 mg/g) and β-glucan (31.12 ± 1.76 mg/g) content of SE-cEPS compared to the SU-cEPS was established. Due to the partial dissolution of selenium-cEPS, the antibacterial and antioxidative potential of their soluble fractions (selenium-cEPSsol) were studied. Results indicated that selenium incorporation from different sources into mushroom's exopolysaccharides affected their biological activities. Analysis revealed that all tested Gram-positive and Gram-negative bacterial strains were susceptible to selenium-cEPSsol, with more pronounced microbicidal activity of SE-cEPSsol against Gram-positive bacteria. Although no differences between samples were observed regarding activity on Gram-negative bacteria, selenium incorporation into exopolysaccharides influenced their antibacterial activity against two Escherichia coli species. The HPLC amino acid analysis showed cationic character of selenium-cEPS protein fraction, indicating its potential participation in antimicrobial activities. Selenium-cEPSsol showed significant capacity to reduce the DPPH free radicals and a high ability to chelate Fe2+ ions as a consequence of its ligand characteristic and capability to coordinate with metal ions. Presence of D-rhamnose was confirmed in SE-cEPS, and it has contributed to its higher chelating ability compared to the SU-cEPSsol. The partial dissolution feature of selenium-cEPS samples, as well as established biological activities of the selenium-cEPSsol can be further investigated and applied in the food industry for elimination causatives of the oxidative stress and food-related pathogens.", publisher = "Elsevier Ltd", journal = "Food Bioscience", title = "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor", pages = "101046", volume = "42", doi = "10.1016/j.fbio.2021.101046" }
Miletić, D., Turło, J., Podsadni, P., Sknepnek, A., Szczepanska, A., Klimaszewska, M., Malinowska, E., Lević, S., Nedović, V.,& Nikšić, M.. (2021). Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience Elsevier Ltd., 42, 101046. https://doi.org/10.1016/j.fbio.2021.101046
Miletić D, Turło J, Podsadni P, Sknepnek A, Szczepanska A, Klimaszewska M, Malinowska E, Lević S, Nedović V, Nikšić M. Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor. in Food Bioscience. 2021;42:101046. doi:10.1016/j.fbio.2021.101046 .
Miletić, Dunja, Turło, Jadwiga, Podsadni, Piotr, Sknepnek, Aleksandra, Szczepanska, Agnieszka, Klimaszewska, Marzenna, Malinowska, Eliza, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Production of bioactive selenium enriched crude exopolysaccharides via selenourea and sodium selenite bioconversion using Trametes versicolor" in Food Bioscience, 42 (2021):101046, https://doi.org/10.1016/j.fbio.2021.101046 . .