Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities
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2021
Authors
Klaus, Anita
Wan‑Mohtar, Wan Abd Al Qadr Imad

Nikolić, Biljana

Cvetković, Stefana
Vunduk, Jovana

Article (Published version)

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Four types of mycelial extracts were derived from the airlift liquid fermentation (ALF) of Pleurotus flabellatus, namely
exopolysaccharide (EX), endopolysaccharide (EN), hot water (WE), and hot alkali (AE) extracts. Such extracts were screened
for their active components and biological potential. EN proved to be most effective in inhibition of lipid peroxidation
(
EC50 = 1.71 ± 0.02 mg/mL) and in Cupric ion reducing antioxidant capacity (CUPRAC) assay (
EC50 = 2.91 ± 0.01 mg
TE/g). AE exhibited most pronounced ability to chelate ferrous ions (
EC50 = 4.96 ± 0.08 mg/mL) and to scavenge ABTS
radicals (
EC50 = 3.36 ± 0.03 mg TE/g). β-glucans and total phenols contributed most to the chelating ability and quenching
of ABTS radicals. Inhibition of lipid peroxidation correlated best with total glucans, total proteins, and β-glucans. Total
proteins contributed most to CUPRAC antioxidant capacity. Antifungal effect was determined against Candida albicans
ATCC 10231 (MIC: 0.019–0.62...5 mg/mL; MFC: 0.039–2.5 mg/mL), and towards C. albicans clinical isolate (MIC and MFC:
10.0–20.0 mg/mL). Comparison of cytotoxicity against colorectal carcinoma HCT 116 cells (
IC50: 1.8 ± 0.3–24.6 ± 4.2 mg/
mL) and normal lung MRC-5 fibroblasts (
IC50: 17.0 ± 4.2–42.1 ± 6.1 mg/mL) showed that EN, and especially AE possess
selective anticancer activity (SI values 3.41 and 9.44, respectively). Slight genotoxicity was observed only for AE and EX,
indicating the low risk concerning this feature. Notable antioxidative and anticandidal activities, selective cytotoxicity against
colorectal carcinoma cells, and absence/low genotoxicity pointed out that ALF—cultivated P. flabellatus mycelium could
be considered as a valuable source of bioactive substances.
Keywords:
Antioxidative / Antifungal / Cytotoxicity / Genotoxicity / Pleurotus flabellatusSource:
World Journal of Microbiology and Biotechnology, 2021, 37, 1, 17-Publisher:
- Springer Science and Business Media B.V.
Funding / projects:
DOI: 10.1007/s11274-020-02980-6
ISSN: 0959-3993
WoS: 000606862700004
Scopus: 2-s2.0-85098548235
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Poljoprivredni fakultetTY - JOUR AU - Klaus, Anita AU - Wan‑Mohtar, Wan Abd Al Qadr Imad AU - Nikolić, Biljana AU - Cvetković, Stefana AU - Vunduk, Jovana PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5805 AB - Four types of mycelial extracts were derived from the airlift liquid fermentation (ALF) of Pleurotus flabellatus, namely exopolysaccharide (EX), endopolysaccharide (EN), hot water (WE), and hot alkali (AE) extracts. Such extracts were screened for their active components and biological potential. EN proved to be most effective in inhibition of lipid peroxidation ( EC50 = 1.71 ± 0.02 mg/mL) and in Cupric ion reducing antioxidant capacity (CUPRAC) assay ( EC50 = 2.91 ± 0.01 mg TE/g). AE exhibited most pronounced ability to chelate ferrous ions ( EC50 = 4.96 ± 0.08 mg/mL) and to scavenge ABTS radicals ( EC50 = 3.36 ± 0.03 mg TE/g). β-glucans and total phenols contributed most to the chelating ability and quenching of ABTS radicals. Inhibition of lipid peroxidation correlated best with total glucans, total proteins, and β-glucans. Total proteins contributed most to CUPRAC antioxidant capacity. Antifungal effect was determined against Candida albicans ATCC 10231 (MIC: 0.019–0.625 mg/mL; MFC: 0.039–2.5 mg/mL), and towards C. albicans clinical isolate (MIC and MFC: 10.0–20.0 mg/mL). Comparison of cytotoxicity against colorectal carcinoma HCT 116 cells ( IC50: 1.8 ± 0.3–24.6 ± 4.2 mg/ mL) and normal lung MRC-5 fibroblasts ( IC50: 17.0 ± 4.2–42.1 ± 6.1 mg/mL) showed that EN, and especially AE possess selective anticancer activity (SI values 3.41 and 9.44, respectively). Slight genotoxicity was observed only for AE and EX, indicating the low risk concerning this feature. Notable antioxidative and anticandidal activities, selective cytotoxicity against colorectal carcinoma cells, and absence/low genotoxicity pointed out that ALF—cultivated P. flabellatus mycelium could be considered as a valuable source of bioactive substances. PB - Springer Science and Business Media B.V. T2 - World Journal of Microbiology and Biotechnology T1 - Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities IS - 1 SP - 17 VL - 37 DO - 10.1007/s11274-020-02980-6 ER -
@article{ author = "Klaus, Anita and Wan‑Mohtar, Wan Abd Al Qadr Imad and Nikolić, Biljana and Cvetković, Stefana and Vunduk, Jovana", year = "2021", abstract = "Four types of mycelial extracts were derived from the airlift liquid fermentation (ALF) of Pleurotus flabellatus, namely exopolysaccharide (EX), endopolysaccharide (EN), hot water (WE), and hot alkali (AE) extracts. Such extracts were screened for their active components and biological potential. EN proved to be most effective in inhibition of lipid peroxidation ( EC50 = 1.71 ± 0.02 mg/mL) and in Cupric ion reducing antioxidant capacity (CUPRAC) assay ( EC50 = 2.91 ± 0.01 mg TE/g). AE exhibited most pronounced ability to chelate ferrous ions ( EC50 = 4.96 ± 0.08 mg/mL) and to scavenge ABTS radicals ( EC50 = 3.36 ± 0.03 mg TE/g). β-glucans and total phenols contributed most to the chelating ability and quenching of ABTS radicals. Inhibition of lipid peroxidation correlated best with total glucans, total proteins, and β-glucans. Total proteins contributed most to CUPRAC antioxidant capacity. Antifungal effect was determined against Candida albicans ATCC 10231 (MIC: 0.019–0.625 mg/mL; MFC: 0.039–2.5 mg/mL), and towards C. albicans clinical isolate (MIC and MFC: 10.0–20.0 mg/mL). Comparison of cytotoxicity against colorectal carcinoma HCT 116 cells ( IC50: 1.8 ± 0.3–24.6 ± 4.2 mg/ mL) and normal lung MRC-5 fibroblasts ( IC50: 17.0 ± 4.2–42.1 ± 6.1 mg/mL) showed that EN, and especially AE possess selective anticancer activity (SI values 3.41 and 9.44, respectively). Slight genotoxicity was observed only for AE and EX, indicating the low risk concerning this feature. Notable antioxidative and anticandidal activities, selective cytotoxicity against colorectal carcinoma cells, and absence/low genotoxicity pointed out that ALF—cultivated P. flabellatus mycelium could be considered as a valuable source of bioactive substances.", publisher = "Springer Science and Business Media B.V.", journal = "World Journal of Microbiology and Biotechnology", title = "Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities", number = "1", pages = "17", volume = "37", doi = "10.1007/s11274-020-02980-6" }
Klaus, A., Wan‑Mohtar, W. A. A. Q. I., Nikolić, B., Cvetković, S.,& Vunduk, J.. (2021). Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities. in World Journal of Microbiology and Biotechnology Springer Science and Business Media B.V.., 37(1), 17. https://doi.org/10.1007/s11274-020-02980-6
Klaus A, Wan‑Mohtar WAAQI, Nikolić B, Cvetković S, Vunduk J. Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities. in World Journal of Microbiology and Biotechnology. 2021;37(1):17. doi:10.1007/s11274-020-02980-6 .
Klaus, Anita, Wan‑Mohtar, Wan Abd Al Qadr Imad, Nikolić, Biljana, Cvetković, Stefana, Vunduk, Jovana, "Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor—the evidence of potent in vitro biological activities" in World Journal of Microbiology and Biotechnology, 37, no. 1 (2021):17, https://doi.org/10.1007/s11274-020-02980-6 . .