Application of isoenzymes in selection of low vigour cherry genotypes

Abstract

This study was realized in order to evaluate usability of isoenzymes in selection of low vigour cherry genotypes. The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction. Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes. The results showed that isoenzyme activity could be used in the selection of low vigour cherry genotypes and rootstock for high density orchards.