Application of isoenzymes in selection of low vigour cherry genotypes
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2020
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This study was realized in order to evaluate usability of isoenzymes in selection of low vigour cherry genotypes. The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction. Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes. The results showed that isoenzyme activity could be used i...n the selection of low vigour cherry genotypes and rootstock for high density orchards.
Ključne reči:
Cherry / Cluster analysis / Electrophoresis / Isoenzymes / Low vigour / Prunus sppIzvor:
Acta Horticulturae, 2020, 1289, 125-129Izdavač:
- International Society for Horticultural Science
Finansiranje / projekti:
- The authors gratefully acknowledge the financial support of the Ministry of Education, Science and Technological Development of the Republic of Serbia for this research, as a part of the project TR ? ? ? ? ?.
DOI: 10.17660/ActaHortic.2020.1289.18
ISSN: 0567-7572
WoS: 000706480000018
Scopus: 2-s2.0-85092161484
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Čolić, Slavica AU - Bakić, Ivana AU - Rakonjac, Vera AU - Nikolić, D. AU - Fotirić-Akšić, Milica PY - 2020 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5416 AB - This study was realized in order to evaluate usability of isoenzymes in selection of low vigour cherry genotypes. The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction. Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes. The results showed that isoenzyme activity could be used in the selection of low vigour cherry genotypes and rootstock for high density orchards. PB - International Society for Horticultural Science T2 - Acta Horticulturae T1 - Application of isoenzymes in selection of low vigour cherry genotypes EP - 129 SP - 125 VL - 1289 DO - 10.17660/ActaHortic.2020.1289.18 ER -
@article{ author = "Čolić, Slavica and Bakić, Ivana and Rakonjac, Vera and Nikolić, D. and Fotirić-Akšić, Milica", year = "2020", abstract = "This study was realized in order to evaluate usability of isoenzymes in selection of low vigour cherry genotypes. The correlation of isoenzyme profiles with plant/tree vigour of 20 genotypes belonging to the genus Prunus, subgenus Cerasus as well as to four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14) was investigated in this study. For this purpose, isoenzyme bands were identified by polyacrylamide gel electrophoresis (PAGE) for eight systems: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), shikimate dehydrogenase (SDH) and peroxidase (PRX). Inner bark of one-year-old shoots, in dormant stage was used for protein extraction. Cluster analysis was used to construct dendrogram for the classification of the cherry genotypes based on vigour traits and polymorphism of isoenzymes. The results showed that isoenzyme activity could be used in the selection of low vigour cherry genotypes and rootstock for high density orchards.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "Application of isoenzymes in selection of low vigour cherry genotypes", pages = "129-125", volume = "1289", doi = "10.17660/ActaHortic.2020.1289.18" }
Čolić, S., Bakić, I., Rakonjac, V., Nikolić, D.,& Fotirić-Akšić, M.. (2020). Application of isoenzymes in selection of low vigour cherry genotypes. in Acta Horticulturae International Society for Horticultural Science., 1289, 125-129. https://doi.org/10.17660/ActaHortic.2020.1289.18
Čolić S, Bakić I, Rakonjac V, Nikolić D, Fotirić-Akšić M. Application of isoenzymes in selection of low vigour cherry genotypes. in Acta Horticulturae. 2020;1289:125-129. doi:10.17660/ActaHortic.2020.1289.18 .
Čolić, Slavica, Bakić, Ivana, Rakonjac, Vera, Nikolić, D., Fotirić-Akšić, Milica, "Application of isoenzymes in selection of low vigour cherry genotypes" in Acta Horticulturae, 1289 (2020):125-129, https://doi.org/10.17660/ActaHortic.2020.1289.18 . .