Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR
2020
Аутори
Machado-Moreira, BernardinoMonteiro, Silvia
Santos, Ricardo
Martinez-Murcia, Antonio
Rajković, Andreja
Šmigić, Nada
Richards, Karl G.
Abram, Florence
Burgess, Catherine M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.
Извор:
Journal of Microbiological Methods, 2020, 168Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Protecting the health of Europeans by improving methods for the detection of pathogens in drinking water and water used in food preparation (EU-FP7-311846)
- Teagasc Walsh Fellowship [2015034]
DOI: 10.1016/j.mimet.2019.105786
ISSN: 0167-7012
PubMed: 31770538
WoS: 000508748500008
Scopus: 2-s2.0-85075734123
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Machado-Moreira, Bernardino AU - Monteiro, Silvia AU - Santos, Ricardo AU - Martinez-Murcia, Antonio AU - Rajković, Andreja AU - Šmigić, Nada AU - Richards, Karl G. AU - Abram, Florence AU - Burgess, Catherine M. PY - 2020 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5356 AB - There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water. PB - Elsevier, Amsterdam T2 - Journal of Microbiological Methods T1 - Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR VL - 168 DO - 10.1016/j.mimet.2019.105786 ER -
@article{ author = "Machado-Moreira, Bernardino and Monteiro, Silvia and Santos, Ricardo and Martinez-Murcia, Antonio and Rajković, Andreja and Šmigić, Nada and Richards, Karl G. and Abram, Florence and Burgess, Catherine M.", year = "2020", abstract = "There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.", publisher = "Elsevier, Amsterdam", journal = "Journal of Microbiological Methods", title = "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR", volume = "168", doi = "10.1016/j.mimet.2019.105786" }
Machado-Moreira, B., Monteiro, S., Santos, R., Martinez-Murcia, A., Rajković, A., Šmigić, N., Richards, K. G., Abram, F.,& Burgess, C. M.. (2020). Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. in Journal of Microbiological Methods Elsevier, Amsterdam., 168. https://doi.org/10.1016/j.mimet.2019.105786
Machado-Moreira B, Monteiro S, Santos R, Martinez-Murcia A, Rajković A, Šmigić N, Richards KG, Abram F, Burgess CM. Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. in Journal of Microbiological Methods. 2020;168. doi:10.1016/j.mimet.2019.105786 .
Machado-Moreira, Bernardino, Monteiro, Silvia, Santos, Ricardo, Martinez-Murcia, Antonio, Rajković, Andreja, Šmigić, Nada, Richards, Karl G., Abram, Florence, Burgess, Catherine M., "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR" in Journal of Microbiological Methods, 168 (2020), https://doi.org/10.1016/j.mimet.2019.105786 . .