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Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji

dc.creatorMilošević, Dragana
dc.creatorIgnjatov, Maja
dc.creatorMarjanović-Jeromela, Ana
dc.creatorStanković, Ivana
dc.creatorNikolić, Zorica
dc.creatorTamindžić, Gordana
dc.creatorKrstić, Branka
dc.date.accessioned2020-12-17T22:48:08Z
dc.date.available2020-12-17T22:48:08Z
dc.date.issued2019
dc.identifier.issn1821-3944
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/5234
dc.description.abstractIn 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.en
dc.description.abstractTokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.sr
dc.publisherInstitut za ratarstvo i povrtarstvo, Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31025/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31030/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceRatarstvo i povrtarstvo
dc.subjectDAS-ELTSAen
dc.subjectmustardsen
dc.subjectphylogenetic analysisen
dc.subjectRT-PCRen
dc.subjectTurnip yellows virusen
dc.subjectDAS-ELISAsr
dc.subjectfilogenetska analizasr
dc.subjectRT-PCRsr
dc.subjectslačicasr
dc.subjectTurnip yellows virussr
dc.titleMolecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbiaen
dc.titleMolekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbijisr
dc.typearticle
dc.rights.licenseBY
dc.citation.epage87
dc.citation.issue3
dc.citation.other56(3): 82-87
dc.citation.rankM24
dc.citation.spage82
dc.citation.volume56
dc.identifier.doi10.5937/ratpov56-23514
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/3734/5231.pdf
dc.type.versionpublishedVersion


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