Botrytis squamosa: The causal agent of onion leaf blight in Bosnia and Herzegovina
Botrytis squamosa - prouzrokovač lisne pegavosti luka u Bosni i Herzegovini

2019
Authors
Tanović, Brankica
Koščica, Milan
Hrustić, Jovana

Mihajlović, Milica
Trkulja, Vojislav
Delibašić, Goran

Article (Published version)
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Over the past several decades, necrotic spots, lesions and blight symptoms have been observed on onion leaves in several locations in Bosnia and Herzegovina, where the crop is grown intensively. The type of symptoms indicated a possible infection with Botrytis squamosa, a widespread pathogen of onion. As symptoms of leaf spots and necrotic lesions can also be caused by some other biotic and abiotic factors, our research focused on identifying the causal agent of the observed symptoms. The pathogen was isolated from diseased tissue using standard phytopathological procedure and identified based on pathogenic and morphological features. Identification was confirmed by amplification and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). The influence of temperature and growth medium on mycelial growth rate of the isolates was also studied.
U poslednjih nekoliko godina, na nekoliko lokaliteta u području intenzivne proizvodnje crnog luka u Bosni i Hercegovini uočena je pojava nekrotičnih pega i sušenja lišća. Simptomi oboljenja ukazali su na moguće prisustvo široko rasprostranjenog patogena luka, vrste Botrytis squamosa. S obzirom da pegavost i nekrotične lezije na listu luka mogu biti prouzrokovane različitim biotičkim i abiotičkim faktorima, cilj ovog istraživanja bio je da se precizno identifikuje prouzrokovač oboljenja. Patogen je izolovan primenom standardnih fitopatoloških metoda i identifikovan na osnovu proučenih patogenih i morfoloških karakteristika dobijenih izolata. Identifikacija je potvrđena amplifikacijom i sekvenciranjem ITS rDNA genomnog regiona. Takođe, proučen je uticaj temperature i hranljive podloge na porast izolata.
Keywords:
Botrytis squamosa / onion leaf blight / identification / Bosnia and Herzegovina / Botrytis squamosa / lisna pegavost luka / identifikacija / Bosna i HercegovinaSource:
Pesticidi i fitomedicina, 2019, 34, 1, 9-17Publisher:
- Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
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Poljoprivredni fakultetTY - JOUR AU - Tanović, Brankica AU - Koščica, Milan AU - Hrustić, Jovana AU - Mihajlović, Milica AU - Trkulja, Vojislav AU - Delibašić, Goran PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5226 AB - Over the past several decades, necrotic spots, lesions and blight symptoms have been observed on onion leaves in several locations in Bosnia and Herzegovina, where the crop is grown intensively. The type of symptoms indicated a possible infection with Botrytis squamosa, a widespread pathogen of onion. As symptoms of leaf spots and necrotic lesions can also be caused by some other biotic and abiotic factors, our research focused on identifying the causal agent of the observed symptoms. The pathogen was isolated from diseased tissue using standard phytopathological procedure and identified based on pathogenic and morphological features. Identification was confirmed by amplification and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). The influence of temperature and growth medium on mycelial growth rate of the isolates was also studied. AB - U poslednjih nekoliko godina, na nekoliko lokaliteta u području intenzivne proizvodnje crnog luka u Bosni i Hercegovini uočena je pojava nekrotičnih pega i sušenja lišća. Simptomi oboljenja ukazali su na moguće prisustvo široko rasprostranjenog patogena luka, vrste Botrytis squamosa. S obzirom da pegavost i nekrotične lezije na listu luka mogu biti prouzrokovane različitim biotičkim i abiotičkim faktorima, cilj ovog istraživanja bio je da se precizno identifikuje prouzrokovač oboljenja. Patogen je izolovan primenom standardnih fitopatoloških metoda i identifikovan na osnovu proučenih patogenih i morfoloških karakteristika dobijenih izolata. Identifikacija je potvrđena amplifikacijom i sekvenciranjem ITS rDNA genomnog regiona. Takođe, proučen je uticaj temperature i hranljive podloge na porast izolata. PB - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd T2 - Pesticidi i fitomedicina T1 - Botrytis squamosa: The causal agent of onion leaf blight in Bosnia and Herzegovina T1 - Botrytis squamosa - prouzrokovač lisne pegavosti luka u Bosni i Herzegovini EP - 17 IS - 1 SP - 9 VL - 34 DO - 10.2298/PIF1901009T ER -
@article{ author = "Tanović, Brankica and Koščica, Milan and Hrustić, Jovana and Mihajlović, Milica and Trkulja, Vojislav and Delibašić, Goran", year = "2019", abstract = "Over the past several decades, necrotic spots, lesions and blight symptoms have been observed on onion leaves in several locations in Bosnia and Herzegovina, where the crop is grown intensively. The type of symptoms indicated a possible infection with Botrytis squamosa, a widespread pathogen of onion. As symptoms of leaf spots and necrotic lesions can also be caused by some other biotic and abiotic factors, our research focused on identifying the causal agent of the observed symptoms. The pathogen was isolated from diseased tissue using standard phytopathological procedure and identified based on pathogenic and morphological features. Identification was confirmed by amplification and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). The influence of temperature and growth medium on mycelial growth rate of the isolates was also studied., U poslednjih nekoliko godina, na nekoliko lokaliteta u području intenzivne proizvodnje crnog luka u Bosni i Hercegovini uočena je pojava nekrotičnih pega i sušenja lišća. Simptomi oboljenja ukazali su na moguće prisustvo široko rasprostranjenog patogena luka, vrste Botrytis squamosa. S obzirom da pegavost i nekrotične lezije na listu luka mogu biti prouzrokovane različitim biotičkim i abiotičkim faktorima, cilj ovog istraživanja bio je da se precizno identifikuje prouzrokovač oboljenja. Patogen je izolovan primenom standardnih fitopatoloških metoda i identifikovan na osnovu proučenih patogenih i morfoloških karakteristika dobijenih izolata. Identifikacija je potvrđena amplifikacijom i sekvenciranjem ITS rDNA genomnog regiona. Takođe, proučen je uticaj temperature i hranljive podloge na porast izolata.", publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd", journal = "Pesticidi i fitomedicina", title = "Botrytis squamosa: The causal agent of onion leaf blight in Bosnia and Herzegovina, Botrytis squamosa - prouzrokovač lisne pegavosti luka u Bosni i Herzegovini", pages = "17-9", number = "1", volume = "34", doi = "10.2298/PIF1901009T" }
Tanović, B., Koščica, M., Hrustić, J., Mihajlović, M., Trkulja, V.,& Delibašić, G.. (2019). Botrytis squamosa: The causal agent of onion leaf blight in Bosnia and Herzegovina. in Pesticidi i fitomedicina Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 9-17. https://doi.org/10.2298/PIF1901009T
Tanović B, Koščica M, Hrustić J, Mihajlović M, Trkulja V, Delibašić G. Botrytis squamosa: The causal agent of onion leaf blight in Bosnia and Herzegovina. in Pesticidi i fitomedicina. 2019;34(1):9-17. doi:10.2298/PIF1901009T .
Tanović, Brankica, Koščica, Milan, Hrustić, Jovana, Mihajlović, Milica, Trkulja, Vojislav, Delibašić, Goran, "Botrytis squamosa: The causal agent of onion leaf blight in Bosnia and Herzegovina" in Pesticidi i fitomedicina, 34, no. 1 (2019):9-17, https://doi.org/10.2298/PIF1901009T . .