Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones
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2019
Authors
Fotirić-Akšić, Milica
Guffa, B.
Gašić, Uroš M.

Dabić-Zagorac, Dragana

Natić, Maja

Meland, Mekjell
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The presence of pollinators in orchards is crucial to obtain high fruit set and yields of fruits. Despite the fact that sour cherry cultivars are mainly autogamous, insect visits are still of great importance for their propagation. In order to attract and reward pollinators, flowers have to provide adequate nourishment to them. Besides nectar, bees gather pollen, which are a prerequisite for normal colony growth and development of their broods. 'Oblačinska' sour cherry (Prunus cerasus L.), an autochthonous cultivar, is the most highly planted cultivar in Serbian commercial orchards. Since the cultivar is actually a mixture of different clones, variability in numerous traits and, particularly, its yields has been reported. Since phenolic compounds are considered to be fundamental pollen chemicals, the aim of this study was to determine the phenolic compounds profile in pollen collected from 15 'Oblačinska' sour cherry clones with varying productivity levels. Solid phase extraction (SPE)..., combined with ultra-high-performance liquid chromatography coupled with a diode array detector and a triple quadruple mass spectrometer (UHPLC DAD-MS/MS), was used to analyse the polyphenolic profile of pollen. Among 23 components quantified, rutin was the most abundant phenolic compound. It ranged from 98.49 (clone V/P) to 358.83 mg kg-1 (clone III/9) and was observed to contribute, on average, 56% of the total phenolic compounds in pollen as quantified in different 'Oblačinska' sour cherry clones. In addition to this compound, clones contained significant amounts of chlorogenic acid (12.92%), astragalin (8.19%), and hyperoside (5.59%) as well. Cluster analysis grouped pollen clones in four different clusters, which showed that clones III/9, IV/8, and V/P had unique phenolic profiles. Despite the significant differences among the studied clones, the contents of chlorogenic acid, rutin, naringin, hyperoside, astralgin, and phlorizin were distinguishable between the clusters.
Keywords:
Chlorogenic acid / Cluster analysis / Flower / Rutin / UHPLC DAD-MS/MSSource:
Acta Horticulturae, 2019, 1235, 387-394Publisher:
- International Society for Horticultural Science
Funding / projects:
- The application of new genotypes and technological innovations for the purpose of improvement of fruit-growing and viticultural production (RS-31063)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-172017)
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Poljoprivredni fakultetTY - CONF AU - Fotirić-Akšić, Milica AU - Guffa, B. AU - Gašić, Uroš M. AU - Dabić-Zagorac, Dragana AU - Natić, Maja AU - Meland, Mekjell PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5135 AB - The presence of pollinators in orchards is crucial to obtain high fruit set and yields of fruits. Despite the fact that sour cherry cultivars are mainly autogamous, insect visits are still of great importance for their propagation. In order to attract and reward pollinators, flowers have to provide adequate nourishment to them. Besides nectar, bees gather pollen, which are a prerequisite for normal colony growth and development of their broods. 'Oblačinska' sour cherry (Prunus cerasus L.), an autochthonous cultivar, is the most highly planted cultivar in Serbian commercial orchards. Since the cultivar is actually a mixture of different clones, variability in numerous traits and, particularly, its yields has been reported. Since phenolic compounds are considered to be fundamental pollen chemicals, the aim of this study was to determine the phenolic compounds profile in pollen collected from 15 'Oblačinska' sour cherry clones with varying productivity levels. Solid phase extraction (SPE), combined with ultra-high-performance liquid chromatography coupled with a diode array detector and a triple quadruple mass spectrometer (UHPLC DAD-MS/MS), was used to analyse the polyphenolic profile of pollen. Among 23 components quantified, rutin was the most abundant phenolic compound. It ranged from 98.49 (clone V/P) to 358.83 mg kg-1 (clone III/9) and was observed to contribute, on average, 56% of the total phenolic compounds in pollen as quantified in different 'Oblačinska' sour cherry clones. In addition to this compound, clones contained significant amounts of chlorogenic acid (12.92%), astragalin (8.19%), and hyperoside (5.59%) as well. Cluster analysis grouped pollen clones in four different clusters, which showed that clones III/9, IV/8, and V/P had unique phenolic profiles. Despite the significant differences among the studied clones, the contents of chlorogenic acid, rutin, naringin, hyperoside, astralgin, and phlorizin were distinguishable between the clusters. PB - International Society for Horticultural Science C3 - Acta Horticulturae T1 - Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones EP - 394 SP - 387 VL - 1235 DO - 10.17660/ActaHortic.2019.1235.53 ER -
@conference{ author = "Fotirić-Akšić, Milica and Guffa, B. and Gašić, Uroš M. and Dabić-Zagorac, Dragana and Natić, Maja and Meland, Mekjell", year = "2019", abstract = "The presence of pollinators in orchards is crucial to obtain high fruit set and yields of fruits. Despite the fact that sour cherry cultivars are mainly autogamous, insect visits are still of great importance for their propagation. In order to attract and reward pollinators, flowers have to provide adequate nourishment to them. Besides nectar, bees gather pollen, which are a prerequisite for normal colony growth and development of their broods. 'Oblačinska' sour cherry (Prunus cerasus L.), an autochthonous cultivar, is the most highly planted cultivar in Serbian commercial orchards. Since the cultivar is actually a mixture of different clones, variability in numerous traits and, particularly, its yields has been reported. Since phenolic compounds are considered to be fundamental pollen chemicals, the aim of this study was to determine the phenolic compounds profile in pollen collected from 15 'Oblačinska' sour cherry clones with varying productivity levels. Solid phase extraction (SPE), combined with ultra-high-performance liquid chromatography coupled with a diode array detector and a triple quadruple mass spectrometer (UHPLC DAD-MS/MS), was used to analyse the polyphenolic profile of pollen. Among 23 components quantified, rutin was the most abundant phenolic compound. It ranged from 98.49 (clone V/P) to 358.83 mg kg-1 (clone III/9) and was observed to contribute, on average, 56% of the total phenolic compounds in pollen as quantified in different 'Oblačinska' sour cherry clones. In addition to this compound, clones contained significant amounts of chlorogenic acid (12.92%), astragalin (8.19%), and hyperoside (5.59%) as well. Cluster analysis grouped pollen clones in four different clusters, which showed that clones III/9, IV/8, and V/P had unique phenolic profiles. Despite the significant differences among the studied clones, the contents of chlorogenic acid, rutin, naringin, hyperoside, astralgin, and phlorizin were distinguishable between the clusters.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones", pages = "394-387", volume = "1235", doi = "10.17660/ActaHortic.2019.1235.53" }
Fotirić-Akšić, M., Guffa, B., Gašić, U. M., Dabić-Zagorac, D., Natić, M.,& Meland, M.. (2019). Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones. in Acta Horticulturae International Society for Horticultural Science., 1235, 387-394. https://doi.org/10.17660/ActaHortic.2019.1235.53
Fotirić-Akšić M, Guffa B, Gašić UM, Dabić-Zagorac D, Natić M, Meland M. Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones. in Acta Horticulturae. 2019;1235:387-394. doi:10.17660/ActaHortic.2019.1235.53 .
Fotirić-Akšić, Milica, Guffa, B., Gašić, Uroš M., Dabić-Zagorac, Dragana, Natić, Maja, Meland, Mekjell, "Phenolic profile of pollen collected from different 'Oblačinska' sour cherry (Prunus cerasus L.) clones" in Acta Horticulturae, 1235 (2019):387-394, https://doi.org/10.17660/ActaHortic.2019.1235.53 . .