Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research
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2019
Authors
Kaisarević, SonjaTenji, Dina
Mihajlović, Varja
Micić, Bojana
Francija, Ester
Periz-Stanacev, Jelena
Krnić-Skiljo, Bojana
Brkić, Dragica
Teodorović, Ivana
Article (Published version)
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Show full item recordAbstract
Physiological responses of bacterial, fish, rat and human hepatoma cells to the technical cypermethrin (AS), cypermethrin-based plant protection product (PPP), and the major co-formulant (solvent) were compared. The endpoints included: bioluminescence, total protein content, activity of mitochondrial dehydrogenase and cytochrome P450 (CYP) enzymes CYP1A and CYP1B, and expression of several genes encoding different CYP enzyme isoforms. Toxicity of PPP was compared with the toxicity predicted using concentration addition model. Cypermethrin disturbs the activity of mitochondrial dehydrogenase. Induction of CYP1A1-, CYP1A2- and CYP1B1-associated activity was more pronounced in PPP than in cypermethrin treatment. The predominant biotransformation pathway of cypermethrin is related to Cyp3a1 induction. Deviations between observed and predicted toxicity of PPP indicate synergistic effects of cypermethrin and a solvent. In vitro cellular assays may serve as rapid pre-screening tool and provid...e for a good indication of mixture effects and prompt further in vivo testing of PPPs when really needed.
Keywords:
Co-formulants / CYP induction / Cypermethrin / Hepatocytes / Cytotoxicity / Mode of actionSource:
Environmental Toxicology and Pharmacology, 2019, 65, 31-39Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Endocrine disrupting compounds: reproductive, metabolic, developmental responses and mechanisms of action in selected model organisms and cell lines (RS-MESTD-Basic Research (BR or ON)-173037)
DOI: 10.1016/j.etap.2018.11.007
ISSN: 1382-6689
PubMed: 30502548
WoS: 000457814500006
Scopus: 2-s2.0-85057214365
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Poljoprivredni fakultetTY - JOUR AU - Kaisarević, Sonja AU - Tenji, Dina AU - Mihajlović, Varja AU - Micić, Bojana AU - Francija, Ester AU - Periz-Stanacev, Jelena AU - Krnić-Skiljo, Bojana AU - Brkić, Dragica AU - Teodorović, Ivana PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5073 AB - Physiological responses of bacterial, fish, rat and human hepatoma cells to the technical cypermethrin (AS), cypermethrin-based plant protection product (PPP), and the major co-formulant (solvent) were compared. The endpoints included: bioluminescence, total protein content, activity of mitochondrial dehydrogenase and cytochrome P450 (CYP) enzymes CYP1A and CYP1B, and expression of several genes encoding different CYP enzyme isoforms. Toxicity of PPP was compared with the toxicity predicted using concentration addition model. Cypermethrin disturbs the activity of mitochondrial dehydrogenase. Induction of CYP1A1-, CYP1A2- and CYP1B1-associated activity was more pronounced in PPP than in cypermethrin treatment. The predominant biotransformation pathway of cypermethrin is related to Cyp3a1 induction. Deviations between observed and predicted toxicity of PPP indicate synergistic effects of cypermethrin and a solvent. In vitro cellular assays may serve as rapid pre-screening tool and provide for a good indication of mixture effects and prompt further in vivo testing of PPPs when really needed. PB - Elsevier, Amsterdam T2 - Environmental Toxicology and Pharmacology T1 - Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research EP - 39 SP - 31 VL - 65 DO - 10.1016/j.etap.2018.11.007 ER -
@article{ author = "Kaisarević, Sonja and Tenji, Dina and Mihajlović, Varja and Micić, Bojana and Francija, Ester and Periz-Stanacev, Jelena and Krnić-Skiljo, Bojana and Brkić, Dragica and Teodorović, Ivana", year = "2019", abstract = "Physiological responses of bacterial, fish, rat and human hepatoma cells to the technical cypermethrin (AS), cypermethrin-based plant protection product (PPP), and the major co-formulant (solvent) were compared. The endpoints included: bioluminescence, total protein content, activity of mitochondrial dehydrogenase and cytochrome P450 (CYP) enzymes CYP1A and CYP1B, and expression of several genes encoding different CYP enzyme isoforms. Toxicity of PPP was compared with the toxicity predicted using concentration addition model. Cypermethrin disturbs the activity of mitochondrial dehydrogenase. Induction of CYP1A1-, CYP1A2- and CYP1B1-associated activity was more pronounced in PPP than in cypermethrin treatment. The predominant biotransformation pathway of cypermethrin is related to Cyp3a1 induction. Deviations between observed and predicted toxicity of PPP indicate synergistic effects of cypermethrin and a solvent. In vitro cellular assays may serve as rapid pre-screening tool and provide for a good indication of mixture effects and prompt further in vivo testing of PPPs when really needed.", publisher = "Elsevier, Amsterdam", journal = "Environmental Toxicology and Pharmacology", title = "Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research", pages = "39-31", volume = "65", doi = "10.1016/j.etap.2018.11.007" }
Kaisarević, S., Tenji, D., Mihajlović, V., Micić, B., Francija, E., Periz-Stanacev, J., Krnić-Skiljo, B., Brkić, D.,& Teodorović, I.. (2019). Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research. in Environmental Toxicology and Pharmacology Elsevier, Amsterdam., 65, 31-39. https://doi.org/10.1016/j.etap.2018.11.007
Kaisarević S, Tenji D, Mihajlović V, Micić B, Francija E, Periz-Stanacev J, Krnić-Skiljo B, Brkić D, Teodorović I. Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research. in Environmental Toxicology and Pharmacology. 2019;65:31-39. doi:10.1016/j.etap.2018.11.007 .
Kaisarević, Sonja, Tenji, Dina, Mihajlović, Varja, Micić, Bojana, Francija, Ester, Periz-Stanacev, Jelena, Krnić-Skiljo, Bojana, Brkić, Dragica, Teodorović, Ivana, "Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research" in Environmental Toxicology and Pharmacology, 65 (2019):31-39, https://doi.org/10.1016/j.etap.2018.11.007 . .