Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research
Само за регистроване кориснике
2019
Аутори
Kaisarević, SonjaTenji, Dina
Mihajlović, Varja
Micić, Bojana
Francija, Ester
Periz-Stanacev, Jelena
Krnić-Skiljo, Bojana
Brkić, Dragica
Teodorović, Ivana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Physiological responses of bacterial, fish, rat and human hepatoma cells to the technical cypermethrin (AS), cypermethrin-based plant protection product (PPP), and the major co-formulant (solvent) were compared. The endpoints included: bioluminescence, total protein content, activity of mitochondrial dehydrogenase and cytochrome P450 (CYP) enzymes CYP1A and CYP1B, and expression of several genes encoding different CYP enzyme isoforms. Toxicity of PPP was compared with the toxicity predicted using concentration addition model. Cypermethrin disturbs the activity of mitochondrial dehydrogenase. Induction of CYP1A1-, CYP1A2- and CYP1B1-associated activity was more pronounced in PPP than in cypermethrin treatment. The predominant biotransformation pathway of cypermethrin is related to Cyp3a1 induction. Deviations between observed and predicted toxicity of PPP indicate synergistic effects of cypermethrin and a solvent. In vitro cellular assays may serve as rapid pre-screening tool and provid...e for a good indication of mixture effects and prompt further in vivo testing of PPPs when really needed.
Кључне речи:
Co-formulants / CYP induction / Cypermethrin / Hepatocytes / Cytotoxicity / Mode of actionИзвор:
Environmental Toxicology and Pharmacology, 2019, 65, 31-39Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Ксенобиотици са хормонском активношћу: репродуктивни, метаболички, развојни одговори и механизам дејства код одабраних модел организама и ћелијских линија (RS-MESTD-Basic Research (BR or ON)-173037)
DOI: 10.1016/j.etap.2018.11.007
ISSN: 1382-6689
PubMed: 30502548
WoS: 000457814500006
Scopus: 2-s2.0-85057214365
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Kaisarević, Sonja AU - Tenji, Dina AU - Mihajlović, Varja AU - Micić, Bojana AU - Francija, Ester AU - Periz-Stanacev, Jelena AU - Krnić-Skiljo, Bojana AU - Brkić, Dragica AU - Teodorović, Ivana PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5073 AB - Physiological responses of bacterial, fish, rat and human hepatoma cells to the technical cypermethrin (AS), cypermethrin-based plant protection product (PPP), and the major co-formulant (solvent) were compared. The endpoints included: bioluminescence, total protein content, activity of mitochondrial dehydrogenase and cytochrome P450 (CYP) enzymes CYP1A and CYP1B, and expression of several genes encoding different CYP enzyme isoforms. Toxicity of PPP was compared with the toxicity predicted using concentration addition model. Cypermethrin disturbs the activity of mitochondrial dehydrogenase. Induction of CYP1A1-, CYP1A2- and CYP1B1-associated activity was more pronounced in PPP than in cypermethrin treatment. The predominant biotransformation pathway of cypermethrin is related to Cyp3a1 induction. Deviations between observed and predicted toxicity of PPP indicate synergistic effects of cypermethrin and a solvent. In vitro cellular assays may serve as rapid pre-screening tool and provide for a good indication of mixture effects and prompt further in vivo testing of PPPs when really needed. PB - Elsevier, Amsterdam T2 - Environmental Toxicology and Pharmacology T1 - Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research EP - 39 SP - 31 VL - 65 DO - 10.1016/j.etap.2018.11.007 ER -
@article{ author = "Kaisarević, Sonja and Tenji, Dina and Mihajlović, Varja and Micić, Bojana and Francija, Ester and Periz-Stanacev, Jelena and Krnić-Skiljo, Bojana and Brkić, Dragica and Teodorović, Ivana", year = "2019", abstract = "Physiological responses of bacterial, fish, rat and human hepatoma cells to the technical cypermethrin (AS), cypermethrin-based plant protection product (PPP), and the major co-formulant (solvent) were compared. The endpoints included: bioluminescence, total protein content, activity of mitochondrial dehydrogenase and cytochrome P450 (CYP) enzymes CYP1A and CYP1B, and expression of several genes encoding different CYP enzyme isoforms. Toxicity of PPP was compared with the toxicity predicted using concentration addition model. Cypermethrin disturbs the activity of mitochondrial dehydrogenase. Induction of CYP1A1-, CYP1A2- and CYP1B1-associated activity was more pronounced in PPP than in cypermethrin treatment. The predominant biotransformation pathway of cypermethrin is related to Cyp3a1 induction. Deviations between observed and predicted toxicity of PPP indicate synergistic effects of cypermethrin and a solvent. In vitro cellular assays may serve as rapid pre-screening tool and provide for a good indication of mixture effects and prompt further in vivo testing of PPPs when really needed.", publisher = "Elsevier, Amsterdam", journal = "Environmental Toxicology and Pharmacology", title = "Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research", pages = "39-31", volume = "65", doi = "10.1016/j.etap.2018.11.007" }
Kaisarević, S., Tenji, D., Mihajlović, V., Micić, B., Francija, E., Periz-Stanacev, J., Krnić-Skiljo, B., Brkić, D.,& Teodorović, I.. (2019). Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research. in Environmental Toxicology and Pharmacology Elsevier, Amsterdam., 65, 31-39. https://doi.org/10.1016/j.etap.2018.11.007
Kaisarević S, Tenji D, Mihajlović V, Micić B, Francija E, Periz-Stanacev J, Krnić-Skiljo B, Brkić D, Teodorović I. Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research. in Environmental Toxicology and Pharmacology. 2019;65:31-39. doi:10.1016/j.etap.2018.11.007 .
Kaisarević, Sonja, Tenji, Dina, Mihajlović, Varja, Micić, Bojana, Francija, Ester, Periz-Stanacev, Jelena, Krnić-Skiljo, Bojana, Brkić, Dragica, Teodorović, Ivana, "Comparative analyses of cellular physiological responses of non-target species to cypermethrin and its formulated product: Contribution to mode of action research" in Environmental Toxicology and Pharmacology, 65 (2019):31-39, https://doi.org/10.1016/j.etap.2018.11.007 . .