Clostridium botulinum spores in European honey bees from Serbia
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2019
Authors
Matović, KazimirMisić, Dušan
Karabasil, Nedjeljko
Nedić, Nebojša
Dmitrić, Marko
Jevtić, Goran
Ćirić, Jelena
Article (Published version)
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A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulin...um spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
Keywords:
Apis mellifera / Clostridium botulinum / microbiological methods / PCRSource:
Journal of Apicultural Research, 2019, 58, 3, 420-426Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Bacterial zoonoses-Development of molecular and immunological diagnostic methods and their standardization (RS-MESTD-Technological Development (TD or TR)-31088)
- Improvement of genetic potential and technologies in forage crops production in function of sustainable animal husbandry development (RS-MESTD-Technological Development (TD or TR)-31057)
DOI: 10.1080/00218839.2018.1560654
ISSN: 0021-8839
WoS: 000466809300004
Scopus: 2-s2.0-85061025323
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Poljoprivredni fakultetTY - JOUR AU - Matović, Kazimir AU - Misić, Dušan AU - Karabasil, Nedjeljko AU - Nedić, Nebojša AU - Dmitrić, Marko AU - Jevtić, Goran AU - Ćirić, Jelena PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5064 AB - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined. PB - Taylor & Francis Ltd, Abingdon T2 - Journal of Apicultural Research T1 - Clostridium botulinum spores in European honey bees from Serbia EP - 426 IS - 3 SP - 420 VL - 58 DO - 10.1080/00218839.2018.1560654 ER -
@article{ author = "Matović, Kazimir and Misić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena", year = "2019", abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Journal of Apicultural Research", title = "Clostridium botulinum spores in European honey bees from Serbia", pages = "426-420", number = "3", volume = "58", doi = "10.1080/00218839.2018.1560654" }
Matović, K., Misić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research Taylor & Francis Ltd, Abingdon., 58(3), 420-426. https://doi.org/10.1080/00218839.2018.1560654
Matović K, Misić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426. doi:10.1080/00218839.2018.1560654 .
Matović, Kazimir, Misić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426, https://doi.org/10.1080/00218839.2018.1560654 . .