Приказ основних података о документу

dc.creatorSupramani, Sugenendran
dc.creatorAhmad, Rahayu
dc.creatorIlham, Zul
dc.creatorAnnuar, Mohamad Suffian Mohamad
dc.creatorKlaus, Anita
dc.creatorWan-Mohtar, Wan Abd Al Qadr Imad
dc.date.accessioned2020-12-17T22:35:13Z
dc.date.available2020-12-17T22:35:13Z
dc.date.issued2019
dc.identifier.issn2471-1888
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/5018
dc.description.abstractWild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p lt 0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p lt 0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.en
dc.publisherAmer Inst Mathematical Sciences-Aims, Springfield
dc.relationUniversity of MalayaUniversiti Malaya [BK073-2017, RU003I-2017, FRGS: FP066-2018A]
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceAims Microbiology
dc.subjectGanoderma lucidumen
dc.subjectresponse surface methodologyen
dc.subjectsubmerged-liquid fermentationen
dc.subjectexopolysaccharideen
dc.subjectintracellular polysaccharideen
dc.titleOptimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodologyen
dc.typearticle
dc.rights.licenseBY
dc.citation.epage38
dc.citation.issue1
dc.citation.other5(1): 19-38
dc.citation.spage19
dc.citation.volume5
dc.identifier.doi10.3934/microbiol.2019.1.19
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/3530/5015.pdf
dc.identifier.scopus2-s2.0-85064084285
dc.identifier.pmid31384700
dc.identifier.wos000465000400002
dc.type.versionpublishedVersion


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Приказ основних података о документу